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学者姓名：张曌楠
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Abstract ：
Honey bees are important pollinators that enhance food safety and promote biodiversity. However, honey bees are increasingly threatened by the use of insecticides. Matrine, a plant-derived insecticide, has been used in plants such as pear trees, citrus, and cotton, whose flowers serve as a honey source. Despite matrine's economic significance, its impact on bees is rarely reported. In this study, newly emerged honey bee workers (Apis cerana) were randomly divided into four groups and reared to adulthood within a simulated colony environment with suitable temperatures, humidity, and food sources. The adult workers were exposed to four concentrations (0, 1.2, 10, and 40 mg/L) of matrine. Continuous matrine intake for more than 9 days caused the rapid death of honey bees but had no significant effect on food consumption and body weight of the honey bees. Oral exposure to matrine decreased the abundance of beneficial Gilliamella, Dorea, and Lachnoclostridium in gut microbiota. It also led to the up-regulation of argininosuccinic acid and spermidine, and the down-regulation of arachidonic acid and LOC107998471 (Aldh), which control the decomposition of spermidine. This finding underscored the role of these microbes, metabolites, and genes in honey bees under matrine stress. This study provides a comprehensive understanding of matrine toxicity to honey bees, revealing the crucial roles of microbiota, metabolites, and genes. These findings provide important references for honey bee poisoning and the associated detoxification mechanism.
Keyword ：
Bioinsecticide Bioinsecticide Eastern honey bee Eastern honey bee Honey bee health Honey bee health Matrine Matrine Multiomics Multiomics
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| GB/T 7714 | Yu, Yinglong , Zhang, Zhaonan , Fan, Yuanchan et al. Bioinsecticide matrine causes a cliff-like decline and disorder of gut microbiota, transcriptomics, and metabolomics profiles in honey bees (Apis cerana) [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 216 . |
| MLA | Yu, Yinglong et al. "Bioinsecticide matrine causes a cliff-like decline and disorder of gut microbiota, transcriptomics, and metabolomics profiles in honey bees (Apis cerana)" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 216 (2025) . |
| APA | Yu, Yinglong , Zhang, Zhaonan , Fan, Yuanchan , Zhou, Wencai , Yao, Dan , Zhan, Hongping et al. Bioinsecticide matrine causes a cliff-like decline and disorder of gut microbiota, transcriptomics, and metabolomics profiles in honey bees (Apis cerana) . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 216 . |
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Sacbrood virus (SBV) is a significant problem that impedes brood development in both eastern and western honeybees. Whole-genome sequencing has become an important tool in researching population genetic variations. Numerous studies have been conducted using multiple techniques to suppress SBV infection in honeybees, but the genetic markers and molecular mechanisms underlying SBV resistance have not been identified. To explore single nucleotide polymorphisms (SNPs), insertions, deletions (Indels), and genes at the DNA level related to SBV resistance, we conducted whole-genome resequencing on 90 Apis cerana cerana larvae raised in vitro and challenged with SBV. After filtering, a total of 337.47 gigabytes of clean data and 31,000,613 high-quality SNP loci were detected in three populations. We used ten databases to annotate 9359 predicted genes. By combining population differentiation index (F-ST) and nucleotide polymorphisms (p), we examined genome variants between resistant (R) and susceptible (S) larvae, focusing on site integrity (INT < 0.5) and minor allele frequency (MAF < 0.05). A selective sweep analysis with the top 1% and top 5% was used to identify significant regions. Two SNPs on the 15th chromosome with GenBank KZ288474.1_322717 (Guanine > Cytosine) and KZ288479.1_95621 (Cytosine > Thiamine) were found to be significantly associated with SBV resistance based on their associated allele frequencies after SNP validation. Each SNP was authenticated in 926 and 1022 samples, respectively. The enrichment and functional annotation pathways from significantly predicted genes to SBV resistance revealed immune response processes, signal transduction mechanisms, endocytosis, peroxisomes, phagosomes, and regulation of autophagy, which may be significant in SBV resistance. This study presents novel and useful SNP molecular markers that can be utilized as assisted molecular markers to select honeybees resistant to SBV for breeding and that can be used as a biocontrol technique to protect honeybees from SBV.
Keyword ：
Apis cerana cerana Apis cerana cerana larva larva nucleotide polymorphisms nucleotide polymorphisms population differentiation index population differentiation index sacbrood virus sacbrood virus sequencing sequencing single nucleotide polymorphism single nucleotide polymorphism variants variants
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| GB/T 7714 | Hassanyar, Aqai Kalan , Nie, Hongyi , Li, Zhiguo et al. Discovery of SNP Molecular Markers and Candidate Genes Associated with Sacbrood Virus Resistance in Apis cerana cerana Larvae by Whole-Genome Resequencing [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (7) . |
| MLA | Hassanyar, Aqai Kalan et al. "Discovery of SNP Molecular Markers and Candidate Genes Associated with Sacbrood Virus Resistance in Apis cerana cerana Larvae by Whole-Genome Resequencing" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 7 (2023) . |
| APA | Hassanyar, Aqai Kalan , Nie, Hongyi , Li, Zhiguo , Lin, Yan , Huang, Jingnan , Woldegiorgis, Samuel Tareke et al. Discovery of SNP Molecular Markers and Candidate Genes Associated with Sacbrood Virus Resistance in Apis cerana cerana Larvae by Whole-Genome Resequencing . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (7) . |
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MicroRNAs (miRNAs) play a vital role in the nerve regulation of honey bees (Apis mellifera). This study aims to investigate the differences in expression of miRNAs in a honey bee's brain for olfactory learning tasks and to explore their potential role in a honey bee's olfactory learning and memory. In this study, 12 day old honey bees with strong and weak olfactory performances were utilized to investigate the influence of miRNAs on olfactory learning behavior. The honey bee brains were dissected, and a small RNA-seq technique was used for high-throughput sequencing. The data analysis of the miRNA sequences revealed that 14 differentially expressed miRNAs (DEmiRNAs) between the two groups, strong (S) and weak (W), for olfactory performance in honey bees were identified, which included seven up-regulated and seven down-regulated. The qPCR verification results of the 14 miRNAs showed that four miRNAs (miR-184-3p, miR-276-3p, miR-87-3p, and miR-124-3p) were significantly associated with olfactory learning and memory. The target genes of these DEmiRNAs were subjected to the GO database annotation and KEGG pathway enrichment analyses. The functional annotation and pathway analysis showed that the neuroactive ligand-receptor interaction pathway, oxidative phosphorylation, biosynthesis of amino acids, pentose phosphate pathway, carbon metabolism, and terpenoid backbone biosynthesis may be a great important pathway related to olfactory learning and memory in honey bees. Our findings together further explained the relationship between olfactory performance and the brain function of honey bees at the molecular level and provides a basis for further study on miRNAs related to olfactory learning and memory in honey bees.
Keyword ：
Apis mellifera Apis mellifera brain brain miRNAs miRNAs olfactory performance olfactory performance proboscis extension response (PER) proboscis extension response (PER)
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| GB/T 7714 | Huang, Jingnan , Wang, Tianbao , Qiu, Yuanmei et al. Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera) [J]. | GENES , 2023 , 14 (5) . |
| MLA | Huang, Jingnan et al. "Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera)" . | GENES 14 . 5 (2023) . |
| APA | Huang, Jingnan , Wang, Tianbao , Qiu, Yuanmei , Hassanyar, Aqai Kalan , Zhang, Zhaonan , Sun, Qiaoling et al. Differential Brain Expression Patterns of microRNAs Related to Olfactory Performance in Honey Bees (Apis mellifera) . | GENES , 2023 , 14 (5) . |
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The honey bee dance communication system is one of the most intriguing animal communication signals. It allows foragers to share information related to food sources with nestmates using the waggle dance, which involves the transmission of location information through orientation and duration. Honey bee transcriptomes are dynamic with different stages, expressing an ensemble of differential genes that give rise to substantial behavior diversity. Here, we have provided a global view of mRNA expression profiles in three different stages of waggle dancers including dancing start (DS), dancing end (DE), and dancing cessation (DC). This study yielded 212 differentially expressed genes (DEGs). Among them, we identified 92 DEGs between DS and DC, 118 DEGs between DE and DC, and 2 DEGs between DS and DE. Further, gene ontology (GO) and KEGG analysis suggested that hormone-mediated signaling pathways and participation in pathways controlling the circadian rhythm, nicotinate, and nicotinamide metabolism were attributed to upregulated genes in DS. In addition, the D1 dopamine receptor in dopaminergic pathways, the peptide signaling involving apidaecin, and neprilysin-2 may play important roles in intricate behavioral processes.
Keyword ：
brain brain dance communication dance communication honey bees honey bees mRNA mRNA waggle dance waggle dance
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| GB/T 7714 | Yu, Kejun , Zhao, Yuanhong , Nie, Hongyi et al. Waggle dance-related gene expression profiles in the honey bee (Apis mellifera) brain [J]. | ANIMAL RESEARCH AND ONE HEALTH , 2023 , 1 (2) : 156-167 . |
| MLA | Yu, Kejun et al. "Waggle dance-related gene expression profiles in the honey bee (Apis mellifera) brain" . | ANIMAL RESEARCH AND ONE HEALTH 1 . 2 (2023) : 156-167 . |
| APA | Yu, Kejun , Zhao, Yuanhong , Nie, Hongyi , Wu, Zun , Geng, Long , Huang, Jingnan et al. Waggle dance-related gene expression profiles in the honey bee (Apis mellifera) brain . | ANIMAL RESEARCH AND ONE HEALTH , 2023 , 1 (2) , 156-167 . |
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Simple Summary Dance behaviour of honey bee (Apis mellifera) is highly sophisticated and a unique behavioural pattern that ensures effective and high-quality communication of food information. Honey bee dance behaviour has been discovered and elaborated for decades, but the regulatory mechanism underlying this behaviour is still unclear. In this study, by varying the food quality, such as the concentration of sucrose solution, we successfully manipulated the dance behaviour of honey bees. Then we investigated the effect of long non-coding RNAs (lncRNAs) and their target genes in honey bee brains on waggle dance. The results indicated that lncRNAs in brains of waggle dancers and non-dancing bees exhibited significant differences. Furthermore, lncRNA-mRNA association analysis showed that signal transduction in the brain may be participated in the modulation of waggle dance. Our findings suggested that neurotransmitters presumably served as messengers in the waggle dance. It is the first time that the waggle dance in the honey bee is studied from the perspective of long non-coding RNA. Taken together, this study is expected to provide a new pathway to explore the relationship between behaviour and brain. The ethological study of dance behaviour has yielded some findings since Karl Von Frisch discovered and interpreted the 'dance language' in the honey bee. However, the function and role of long non-coding RNAs on dance behaviour are hardly known until now. In this study, the differential expression patterns of lncRNAs in the brains of waggling dancers and non-dancing bees were analysed by RNA sequencing. Furthermore, lncRNA-mRNA association analysis was constructed to decipher the waggle dance. The results of RNA sequencing indicated that a total of 2877 lncRNAs and 9647 mRNAs were detected from honey bee brains. Further comparison analysis displayed that two lncRNAs, MSTRG.6803.3 and XR_003305156.1, may be involved in the waggle dance. The lncRNA-mRNA association analysis showed that target genes of differentially expressed lncRNAs in the brains between waggling dancers and non-dancing bees were mainly annotated in biological processes related to metabolic process, signalling and response to stimulus and in molecular function associated with signal transducer activity, molecular transducer activity and binding. Nitrogen metabolism was likely implicated in the modulation of the waggle dance. Our findings contribute to further understanding the occurrence and development of waggle dance.
Keyword ：
brain brain honey bee honey bee lncRNA-mRNA association analysis lncRNA-mRNA association analysis long non-coding RNA long non-coding RNA waggle dance waggle dance
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| GB/T 7714 | Feng, Wangjiang , Huang, Jingnan , Zhang, Zhaonan et al. Understanding of Waggle Dance in the Honey Bee (Apis mellifera) from the Perspective of Long Non-Coding RNA [J]. | INSECTS , 2022 , 13 (2) . |
| MLA | Feng, Wangjiang et al. "Understanding of Waggle Dance in the Honey Bee (Apis mellifera) from the Perspective of Long Non-Coding RNA" . | INSECTS 13 . 2 (2022) . |
| APA | Feng, Wangjiang , Huang, Jingnan , Zhang, Zhaonan , Nie, Hongyi , Lin, Yan , Li, Zhiguo et al. Understanding of Waggle Dance in the Honey Bee (Apis mellifera) from the Perspective of Long Non-Coding RNA . | INSECTS , 2022 , 13 (2) . |
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The biological bases of wanting have been characterized in mammals, but whether an equivalent wanting system exists in insects remains unknown. In this study, we focused on honey bees, which perform intensive foraging activities to satisfy colony needs, and sought to determine whether foragers leave the hive driven by specific expectations about reward and whether they recollect these expectations during their waggle dances. We monitored foraging and dance behavior and simultaneously quantified and interfered with biogenic amine signaling in the bee brain. We show that a dopamine-dependent wanting system is activated transiently in the bee brain by increased appetite and individual recollection of profitable food sources, both en route to the goal and during waggle dances. Our results show that insects share with mammals common neural mechanisms for encoding wanting of stimuli with positive hedonic value.
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| GB/T 7714 | Huang, Jingnan , Zhang, Zhaonan , Feng, Wangjiang et al. Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain [J]. | SCIENCE , 2022 , 376 (6592) : 508-, . |
| MLA | Huang, Jingnan et al. "Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain" . | SCIENCE 376 . 6592 (2022) : 508-, . |
| APA | Huang, Jingnan , Zhang, Zhaonan , Feng, Wangjiang , Zhao, Yuanhong , Aldanondo, Anna , Sanchez, Maria Gabriela de Brito et al. Food wanting is mediated by transient activation of dopaminergic signaling in the honey bee brain . | SCIENCE , 2022 , 376 (6592) , 508-, . |
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【目的】对不同行为表现的意大利蜜蜂Apis mellifera ligustica工蜂脑部多巴胺及其受体基因进行检测。【方法】从蜜蜂观察箱中采集不同行为表现的工蜂,解剖其脑部,用高效液相色谱-电化学检测器和实时荧光PCR仪分别检测多巴胺含量和受体基因相对表达量,并进行计算分析研究。【结果】建立了不同行为表现的蜜蜂脑部多巴胺及受体基因的研究方法。意大利蜜蜂哺育蜂脑部的多巴胺含量与新出房工蜂、采集蜂、休息状态下的工蜂存在显著差异。多巴胺受体1基因及多巴胺转运体基因在哺育蜂脑部处于高表达状态,4种不同分工的意大利蜜蜂脑部多巴胺受体2基因、受体3基因相对表达量差异不显著。【结论】多巴胺与蜜蜂不同行为表现密切相关,多巴胺受体1基因与转运体基因可能参与蜜蜂哺育行为。
Keyword ：
基因 基因 多巴胺 多巴胺 意大利蜜蜂 意大利蜜蜂 神经递质 神经递质 行为 行为 高效液相色谱-电化学检测 高效液相色谱-电化学检测
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| GB/T 7714 | 张曌楠 , 黄景南 , 封王江 et al. 不同行为表现的蜜蜂脑部多巴胺及其受体基因的检测分析 [J]. | 应用昆虫学报 , 2019 , 56 (06) : 1292-1298 . |
| MLA | 张曌楠 et al. "不同行为表现的蜜蜂脑部多巴胺及其受体基因的检测分析" . | 应用昆虫学报 56 . 06 (2019) : 1292-1298 . |
| APA | 张曌楠 , 黄景南 , 封王江 , 余岢骏 , 李志国 , 聂红毅 et al. 不同行为表现的蜜蜂脑部多巴胺及其受体基因的检测分析 . | 应用昆虫学报 , 2019 , 56 (06) , 1292-1298 . |
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<正>放蜂与放牧大同小异。同样是都需要转地,不同的是牛羊可以悠哉悠哉逐水草而居,蜜蜂不可以,必须争分夺秒,赶花夺蜜,与时间赛跑。正所谓四季转场,逐花而居。正是由于蜂行业的特殊性,催生了养蜂车这一特殊交通运输工具。如同运输油料的罐车,运输水泥的搅拌车,从某种程度上来说养蜂车上装载的蜜蜂也属于危险品。养蜂车本质
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| GB/T 7714 | 张曌楠 , 杨萌 . 养蜂车发展历史与现状 [J]. | 中国蜂业 , 2019 , 70 (11) : 41-42 . |
| MLA | 张曌楠 et al. "养蜂车发展历史与现状" . | 中国蜂业 70 . 11 (2019) : 41-42 . |
| APA | 张曌楠 , 杨萌 . 养蜂车发展历史与现状 . | 中国蜂业 , 2019 , 70 (11) , 41-42 . |
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对意蜂幼虫肠道组织基因进行深度RNA测序,并对高表达基因进行GO注释和KEGG分析,旨在揭示意蜂幼虫免疫抗病机制。分别取3、4、5、6日龄意蜂幼虫中肠组织抽提RNA,合成cDNA。得到的总读段数都在26 715 638条以上,有效数据达到97%以上,且测序饱和度良好,说明测序所得数据真实可信。将测序有效数据和意蜂公布DNA数据库进行比对,发现意蜂幼虫肠道中有13 789个基因有不同程度表达。选取各样本FPKM排序最前且表达水平最高的100个基因进行GO注释,这里主要涉及细胞组分、分子功能和生物学进程功能分析。利用KEGG分析显示这100个高表达基因分布在能量代谢、翻译、组装和降解以及信号转导等多条通路上。初步分析相应共有基因和特有基因在相关通路上的作用,并对意蜂幼虫肠道受球囊菌胁迫后的相关免疫机制进行一定分析。
Keyword ：
RNA seq RNA seq 幼虫肠道 幼虫肠道 意大利蜜蜂 意大利蜜蜂 高表达基因 高表达基因
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| GB/T 7714 | 熊翠玲 , 黄枳腱 , 梁勤 et al. 基于RNA-seq技术分析意大利蜜蜂幼虫肠道响应球囊菌胁迫的高表达基因 [J]. | 江苏农业科学 , 2018 , 46 (13) : 29-35 . |
| MLA | 熊翠玲 et al. "基于RNA-seq技术分析意大利蜜蜂幼虫肠道响应球囊菌胁迫的高表达基因" . | 江苏农业科学 46 . 13 (2018) : 29-35 . |
| APA | 熊翠玲 , 黄枳腱 , 梁勤 , 徐细建 , 张曌楠 , 骆群 et al. 基于RNA-seq技术分析意大利蜜蜂幼虫肠道响应球囊菌胁迫的高表达基因 . | 江苏农业科学 , 2018 , 46 (13) , 29-35 . |
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Abstract ：
蜜蜂中枢神经系统中的神经递质调控其复杂的生命活动。蜜蜂脑部生物胺类神经递质对生殖发育、生命节律、生活特性等方面都具有十分重要的调控作用。本文对多巴胺能神经系统在蜜蜂中的研究进展进行归纳梳理,包括蜜蜂脑部多巴胺、多巴胺转运体、多巴胺受体以及相关的基因表达等,为研究调控蜜蜂行为的多巴胺分子机制提供借鉴。
Keyword ：
受体 受体 多巴胺 多巴胺 神经递质 神经递质 蜜蜂 蜜蜂 转运体 转运体
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| GB/T 7714 | 黄景南 , 张曌楠 , 和静芳 et al. 蜜蜂脑部多巴胺神经通路研究进展 [J]. | 中国蜂业 , 2018 , 69 (04) : 69-72 . |
| MLA | 黄景南 et al. "蜜蜂脑部多巴胺神经通路研究进展" . | 中国蜂业 69 . 04 (2018) : 69-72 . |
| APA | 黄景南 , 张曌楠 , 和静芳 , 李志国 , 朱雅楠 , 余岢骏 et al. 蜜蜂脑部多巴胺神经通路研究进展 . | 中国蜂业 , 2018 , 69 (04) , 69-72 . |
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