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学者姓名：兰思仁
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Genes that introgress between species can influence the evolutionary and ecological fate of recipients exposed to novel environments. However, key questions on the patterns and molecular mechanisms of introgression in perennial herbaceous plants, which enable distantly related invasive species to thrive in extreme habitats, remain largely unanswered. Here, we report unidirectional introgression from the local species Dendrobium huoshanense to the distantly related invasive species Dendrobium catenatum (Den-drobium officinale) in lithophytic habitats of eastern China. The introgressed regions, which comprise approximately 1% of the genome, contain genes that regulate responses to drought, cold, and metal-ion stresses. Notably, introgressed loci such as CDPK, HHP, PIF, BRI1, and FYshow distinct selection signatures and differential expression compared with their paralogs, each playing a distinct role in drought and cold-stress responses. In addition, CIPK23, PDR9, and HAM demonstrate differential expression relative to their paralogous genes and alleles within introgressed loci, indicating their potential involvement in responses to metal-ion stress. Introgression thus facilitates the colonization of arid, metal-enriched sedimentary habitats by D. catenatum. These findings enhance our understanding of Orchidaceae evolution and reveal the evolutionary role of unidirectional introgression in the adaptation of perennial herbaceous plants to extreme environments.
Keyword ：
Dendrobium Dendrobium distantly related species distantly related species introgression introgression invasive species invasive species metal-ion stress metal-ion stress
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| GB/T 7714 | Jiao Zhenbin , Ren Zhiyao , Hu Chao et al. Unidirectional genomic introgression facilitates the colonization of an invasive orchid in arid, metal-enriched sedimentary habitats [J]. | PLANT COMMUNICATIONS , 2026 , 7 (1) . |
| MLA | Jiao Zhenbin et al. "Unidirectional genomic introgression facilitates the colonization of an invasive orchid in arid, metal-enriched sedimentary habitats" . | PLANT COMMUNICATIONS 7 . 1 (2026) . |
| APA | Jiao Zhenbin , Ren Zhiyao , Hu Chao , Ma Xiaokai , Zhang Guo-Qiang , Chen Li-Jun et al. Unidirectional genomic introgression facilitates the colonization of an invasive orchid in arid, metal-enriched sedimentary habitats . | PLANT COMMUNICATIONS , 2026 , 7 (1) . |
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BackgroundOrchids are well-known for their rich diversity of species as well as wide range habitats. Their floral structures are so unique in angiosperms that many of orchids are economically and culturally important in human society. Orchids pollination strategy and evolutionary trajectory are also fantastic human for centuries. Previously, OrchidBase was created not only for storage and management of orchid genomic and transcriptomic information including Apostasia shenzhenica, Dendrobium catenatum, Phalaenopsis equestris, and two species of Platanthera that belong to three different subfamilies of Orchidaceae, but explored orchid genetic sequences for their function. The OrchidBase offers an opportunity for the plant science community to compare orchid genomes and transcriptomes, and retrieve orchid sequences for further study.DescriptionRecently, three whole-genome sequences of the Epidendroideae species, Cymbidium sinense, C. ensifolium and C. goeringii, were sequenced de novo, assembled, and analyzed. In addition, the systemic transcriptomes of these three species have been established. We included these datasets to develop a new version of OrchidBase 6.0. Furthermore, four new analytical methods, namely regulation, updated transcriptome, advanced BLAST, and domain search, were developed for orchid genome analyses.ConclusionOrchidBase 6.0 extended genetic information to that of eight orchid species and created new tools for an expanded community curation in response to the ever-increasing volume and complexity of data.
Keyword ：
Cymbidium Cymbidium Genome Genome Orchid Orchid OrchidBase OrchidBase Transcriptome Transcriptome Whole-genome sequencing Whole-genome sequencing
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| GB/T 7714 | Chen, You-Yi , Sun, Ye , Li, Chung-, I et al. OrchidBase 6.0: increasing the number of Cymbidium (Orchidaceae) genomes and new bioinformatic tools for orchid genome analysis [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
| MLA | Chen, You-Yi et al. "OrchidBase 6.0: increasing the number of Cymbidium (Orchidaceae) genomes and new bioinformatic tools for orchid genome analysis" . | BMC PLANT BIOLOGY 25 . 1 (2025) . |
| APA | Chen, You-Yi , Sun, Ye , Li, Chung-, I , Lin, Shao-Ting , Zheng, Hao-Chen , Zhang, Zhe-Bin et al. OrchidBase 6.0: increasing the number of Cymbidium (Orchidaceae) genomes and new bioinformatic tools for orchid genome analysis . | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
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Populations of Bletilla striata, an essential traditional Chinese medicinal plant, are undergoing drastic declines mainly driven by anthropogenic pressures and climate change. Such declines have important demographic effects, potentially reducing the genetic diversity and gene flow while pushing this species into an extinction vortex. To develop comprehensive conservation and utilization strategies for B. striata, it is fundamental to understand its genetic landscape first. This study aims to assess the genetic diversity and population structure of B. striata to inform conservation strategies using eight nuclear microsatellites (nrSSR) loci and three chloroplast (cpDNA) loci. Results from nrSSR revealed that western populations exhibited 35.6 % higher allelic richness compared to southern populations, while cpDNA analysis showed southern populations exhibited three times higher haplotype diversity compared to western populations. Allelic richness is not strongly influenced by latitude (P = 0.737), but haplotype diversity decreased by 71.4 % with increasing latitude (P = 0.041), indicating that haplotype diversity is strongly influenced by limited dispersal northward. Considering the historical migration and cultivation practices in China, we speculated that artificial planting and breeding have increased allelic richness in western populations but not haplotype diversity, possibly due to strong pollen flow between cultivated and natural populations. This study highlights the need for targeted conservation efforts in the lower ranges of the Yangtze River and southern China, focusing on preserving the high haplotype diversity of B. striata.
Keyword ：
Bletilla striata Bletilla striata Genetic diversity Genetic diversity Medicinal orchid Medicinal orchid Molecular markers Molecular markers Prioritize protection Prioritize protection
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| GB/T 7714 | Huang, Weichang , Hu, Chao , Zeng, Xinhua et al. Unveiling the genetic landscape of Bletilla striata: conservation challenges in a medicinal orchid under threat [J]. | GLOBAL ECOLOGY AND CONSERVATION , 2025 , 59 . |
| MLA | Huang, Weichang et al. "Unveiling the genetic landscape of Bletilla striata: conservation challenges in a medicinal orchid under threat" . | GLOBAL ECOLOGY AND CONSERVATION 59 (2025) . |
| APA | Huang, Weichang , Hu, Chao , Zeng, Xinhua , Gaitan-Espitia, Juan Diego , Jiang, Kai , Lan, Siren . Unveiling the genetic landscape of Bletilla striata: conservation challenges in a medicinal orchid under threat . | GLOBAL ECOLOGY AND CONSERVATION , 2025 , 59 . |
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Rural cultural ecosystem services (CES) play a pivotal role in enhancing rural well-being. This paper examines the spatial distribution patterns and influencing factors of rural CES in Fujian Province using the biterm topic model and geodetector. The results reveal that rural CES in Fujian Province comprise five categories: culture, leisure, aesthetic, spirit, and science, among which leisure, culture, and aesthetic categories are the most easily perceived by the public. In terms of spatial distribution, the northwestern regions of Fujian Province exhibit better than the southeastern regions in terms of culture, while the latter excel in terms of leisure. Regarding aesthetics, there is an overall trend of extensive dispersion with localized concentrations, with high-value areas primarily located in the southwestern region and the eastern coastal region of Ningde City. The high value areas in the spirit are concentrated in the central and northern regions of Fujian Province. The spatial distribution of science is relatively scattered, with more high-value areas observed in Sanming City and Fuzhou City. Road density, light index, and per capita disposable income demonstrate strong explanatory power in rural CES. These findings can guide targeted development of regional cultural services and address resource mismatches in rural areas of Fujian Province, thereby enhancing the well-being of rural residents.
Keyword ：
BTM BTM Cultural ecosystem service Cultural ecosystem service Geodetector Geodetector Influencing factors Influencing factors Rural ecosystem Rural ecosystem Spatial distribution Spatial distribution
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| GB/T 7714 | Li, Congzhi , Pan, Ying , Yin, Weilun et al. Spatial distribution and influencing factors of rural cultural ecosystem services: a case study of Fujian, China [J]. | SCIENTIFIC REPORTS , 2025 , 15 (1) . |
| MLA | Li, Congzhi et al. "Spatial distribution and influencing factors of rural cultural ecosystem services: a case study of Fujian, China" . | SCIENTIFIC REPORTS 15 . 1 (2025) . |
| APA | Li, Congzhi , Pan, Ying , Yin, Weilun , Lan, Siren . Spatial distribution and influencing factors of rural cultural ecosystem services: a case study of Fujian, China . | SCIENTIFIC REPORTS , 2025 , 15 (1) . |
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A new species of Cymbidium (Orchidaceae) from Hubei Province, China, is described as C. huikanran, which is like C. kanran and C. faberi. Morphologically, it can be distinguished from the former by its basally non-articulate leaves, and arching, short scape; from the latter by its narrowly oblong-lanceolate sepals and narrowly ovate-lanceolate petals. Molecular analysis based on nuclear ribosomal ITS and the plastid sequences (matK and rbcL) clearly indicated that is a new species as a sister to C. faberi in C. subgenus Jensoa.
Keyword ：
Chinese orchids Chinese orchids Cymbidiinae Cymbidiinae Cymbidium subgenus Jensoa Cymbidium subgenus Jensoa new species new species
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| GB/T 7714 | Li, Jun , Zeng, Meng-yao , Chen, Gui-zhen et al. Cymbidium huikanran (Orchidaceae; Epidendroideae), a new species from China: evidence from morphology and molecular analyses [J]. | PHYTOTAXA , 2025 , 683 (2) : 177-183 . |
| MLA | Li, Jun et al. "Cymbidium huikanran (Orchidaceae; Epidendroideae), a new species from China: evidence from morphology and molecular analyses" . | PHYTOTAXA 683 . 2 (2025) : 177-183 . |
| APA | Li, Jun , Zeng, Meng-yao , Chen, Gui-zhen , Li, Yuan-yuan , Liu, Zhong-jian , Lan, Siren . Cymbidium huikanran (Orchidaceae; Epidendroideae), a new species from China: evidence from morphology and molecular analyses . | PHYTOTAXA , 2025 , 683 (2) , 177-183 . |
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Angraecum sesquipedale, also known as Darwin's orchid, possesses an exceptionally long nectar spur. Charles Darwin predicted the orchid to be pollinated by a hawkmoth with a correspondingly long proboscis, later identified as Xanthopan praedicta. In this plant-pollinator interaction, the A. sesquipedale flower emits a complex blend of scent compounds dominated by diurnally regulated oximes (R1R2C = N-OH) to attract crepuscular and nocturnal pollinators. The molecular mechanism of oxime biosynthesis remains unclear in orchids. Here, we present the chromosome-level genome of A. sesquipedale. The haploid genome size is 2.10 Gb and represents 19 pseudochromosomes. Cytochrome P450 encoding genes of the CYP79 family known to be involved in oxime biosynthesis in seed plants are not present in the A. sesquipedale genome nor the genomes of other members of the orchid family. Metabolomic analysis of the A. sesquipedale flower revealed a substantial release of oximes at dusk during the blooming stage. By integrating metabolomic and transcriptomic correlation approaches, flavin-containing monooxygenases (FMOs) encoded by six tandem-repeat genes in the A. sesquipedale genome are identified as catalyzing the formation of oximes present. Further in vitro and in vivo assays confirm the function of FMOs in the oxime biosynthesis. We designate these FMOs as orchid oxime synthases 1-6. The evolutionary aspects related to the CYP79 gene losses and neofunctionalization of FMO-catalyzed biosynthesis of oximes in Darwin's orchid provide new insights into the convergent evolution of biosynthetic pathways.
Keyword ：
Angraecum sesquipedale Angraecum sesquipedale convergent evolution convergent evolution CYP79 absence CYP79 absence flavin monooxygenase flavin monooxygenase white floral scent white floral scent whole genome sequence whole genome sequence
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| GB/T 7714 | Jiang, Kai , Moller, Birger Lindberg , Luo, Shaofan et al. Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid [J]. | MOLECULAR PLANT , 2025 , 18 (3) : 392-415 . |
| MLA | Jiang, Kai et al. "Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid" . | MOLECULAR PLANT 18 . 3 (2025) : 392-415 . |
| APA | Jiang, Kai , Moller, Birger Lindberg , Luo, Shaofan , Yang, Yu , Nelson, David R. , Neilson, Elizabeth Heather Jakobsen et al. Genomic, transcriptomic, and metabolomic analyses reveal convergent evolution of oxime biosynthesis in Darwin's orchid . | MOLECULAR PLANT , 2025 , 18 (3) , 392-415 . |
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The fully mycoheterotrophic orchid Gastrodia Brown (1810: 330; Gastrodieae, Epidendroideae) comprises 103 species (POWO, 2024) and is widespread from India, southern China throughout Southeast Asia to Japan, eastern Australia, New Zealand, Pacific islands, Madagascar-Mascarene Islands and tropical Africa (Pridgeon et al. 2005, Chase et al. 2015, Chen et al. 2023). In China the members of this genus are distributed mainly in southern China, including Fujian, Guangxi, Guangdong, Hong Kong, Hainan, Yunnan and Taiwan (Liu et al. 2021, Zhou et al. 2021, Huang et al. 2021). On Hainan Island, four species, namely Gastrodia longitubularis Meng, Song & Luo (2007: 23), G. punctata Aver. (2006: 21, Lu et al. 2017), G. menghaiensis Tsi & Chen (2021: 703), and G. bawanglingensis Z.H.Chen, Z.Y.Zhang & X.Q.Song in Chen et al. (2023: 39) have been reported from the tropical rainforest. Here, we add another new species to this genus. This new species was found during our field investigation in the karst forests of Hainan Island. After undertaking a review of the literature (Meng et al. 2008, Chen et al. 2009, Chen et al. 2023, POWO 2024), we confirmed that it is a new species, described below.
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| GB/T 7714 | Huang, Ming-zhong , Wei, Chunyi , Li, Ruyi et al. Gastrodia exianlingensis (Gastrodieae, Epidendroideae), a new species of Orchidaceae from Hainan, China [J]. | PHYTOTAXA , 2025 , 689 (1) : 158-162 . |
| MLA | Huang, Ming-zhong et al. "Gastrodia exianlingensis (Gastrodieae, Epidendroideae), a new species of Orchidaceae from Hainan, China" . | PHYTOTAXA 689 . 1 (2025) : 158-162 . |
| APA | Huang, Ming-zhong , Wei, Chunyi , Li, Ruyi , Tu, Xiongde , Zhao, Xuewei , Lan, Siren et al. Gastrodia exianlingensis (Gastrodieae, Epidendroideae), a new species of Orchidaceae from Hainan, China . | PHYTOTAXA , 2025 , 689 (1) , 158-162 . |
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Plant root-associated fungal communities play a pivotal role in enhancing plant growth, nutrient absorption, disease resistance and environmental stress adaptation. Despite their importance, the assembly processes of these communities remain inadequately explored. In this study, we utilizzzed high-throughput sequencing, co-occurrence network analysis and null models to examine the diversity, composition, interaction patterns and assembly mechanisms of the root-associated fungal communities of Mussaenda pubescens, a drought-tolerant shrub that thrives in stressful environments and is widely used for Chinese medicine. Our findings revealed pronounced regional and ecological niche-based variations in the diversity and assembly of total fungi and essential functional guilds, including saprotrophs, symbiotrophs and plant pathogens. Significantly, the fungal diversity of plant pathogens decreased with elevation, whereas total fungi, saprotrophs and symbiotrophs were minimally affected. Stochastic processes, such as dispersal limitation, played a significant role in fungal assembly. Furthermore, soil physicochemical properties, climatic conditions and spatial variables emerged as critical determinants of fungal community structure. This study enriches our understanding of the dynamics governing root-associated fungal community assemblies and underscores the factors essential for sustaining fungal diversity. (sic)(sic) (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic) (sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(Mussaenda pubescens)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)((sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic))(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)((sic)(sic)(sic)(sic)(sic))(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic),(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic)(sic).
Keyword ：
community assembly community assembly co-occurrence network co-occurrence network ecological niches ecological niches fungal communities fungal communities fungal diversity fungal diversity (sic)(sic)(sic)(sic) (sic)(sic)(sic)(sic) (sic)(sic)(sic)(sic)(sic) (sic)(sic)(sic)(sic)(sic) (sic)(sic)(sic)(sic),(sic)(sic)(sic) (sic)(sic)(sic)(sic),(sic)(sic)(sic)
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| GB/T 7714 | Chen, Deqiang , Yang, Juanjuan , Wu, Chunping et al. Drivers of root-associated fungal community structure in a common drought-tolerant shrub at a regional scale [J]. | JOURNAL OF PLANT ECOLOGY , 2025 , 18 (2) . |
| MLA | Chen, Deqiang et al. "Drivers of root-associated fungal community structure in a common drought-tolerant shrub at a regional scale" . | JOURNAL OF PLANT ECOLOGY 18 . 2 (2025) . |
| APA | Chen, Deqiang , Yang, Juanjuan , Wu, Chunping , Li, Linyi , Lan, Siren , Zhang, Dianxiang et al. Drivers of root-associated fungal community structure in a common drought-tolerant shrub at a regional scale . | JOURNAL OF PLANT ECOLOGY , 2025 , 18 (2) . |
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BackgroundPhalaenopsis orchids, belonging to the Orchidaceae family, one of the largest groups of angiosperms, possess significant commercial value due to their fascinating flowers. Petal size is a vital trait that directly determines flower size and shape of Phalaenopsis. However, the genetic and developmental regulation of petal size in Phalaenopsis remains unexplored.ResultsIn this study, we tracked the petal growth pattern through five stages of flower opening, discovering that cell division stops at stage 2 and stages 3 to 5 are the critical periods of rapid petal expansion. RNA-seq was then conducted to further reveal the molecular mechanisms underlying petal size regulation. Gene ontology (GO) analysis indicated that the differentially expressed genes (DEGs) from the four comparable groups were both enriched in terms related to cell expansion. Endogenous hormone assays showed that auxin, cytokinin, and gibberellin were implicated in the petal growth of Phalaenopsis. Moreover, six auxin signaling pathway genes, 11 cell expansion-related genes, and 30 transcription factors (TFs) identified through trend analysis were abundantly expressed during the critical period of petal expansion, suggesting that they may influence petal size by regulating cell expansion. In contrast, 18 TFs exhibited the highest expression levels at the S1 stage, indicating their potential role in petal cell proliferation. Based on weighted gene co-expression network analysis (WGCNA), six hub genes (PaLOF2, PaSWEET11, PaVNI2, PaHDA3, PaPMEI3, PaXTH30) were screened from the green and yellow module which was highly associated with the petal size.ConclusionOur results lay a foundation for further exploration of the molecular mechanisms regulating petal size development and are significant for molecular breeding programs aimed at generating novel Phalaenopsis with desirable traits.
Keyword ：
Cell division Cell division Cell expansion Cell expansion Differentially expressed genes Differentially expressed genes Petal growth Petal growth Phalaenopsis Phalaenopsis RNA-seq RNA-seq
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| GB/T 7714 | Guan, Yunxiao , Zhang, Qiaoyu , Zhang, Ting et al. Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
| MLA | Guan, Yunxiao et al. "Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids" . | BMC PLANT BIOLOGY 25 . 1 (2025) . |
| APA | Guan, Yunxiao , Zhang, Qiaoyu , Zhang, Ting , Li, Minghe , Ai, Ye , Zhai, Junwen et al. Transcriptome analysis reveals the mechanisms underlying petal growth during the flower opening process in Phalaenopsis orchids . | BMC PLANT BIOLOGY , 2025 , 25 (1) . |
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在城市提质建设中，街道的功能性和社会性是其品质优化的关键考量。以福州市鼓楼区为例，综合使用路网、用地功能和街景等数据对街道进行功能分类，并通过百度热力图实现了动态活力模式的划分，基于所得数据，明晰了各类街道的动态活力状态差异。研究发现：鼓楼区以单一型街道为主，其中生活性街道数量最多；鼓楼区街道整体动态活力水平较高，但空间上呈现“东南高，西北低”的分异格局；交通和商业属性是影响居民出行与停留的主要功能因素，生活性街道作为基础型街道很大程度上影响着整体的动态活力水平。同时基于研究结果针对不同类型街道动态活力提升提出了优化策略。
Keyword ：
多源数据 多源数据 精细化设计 精细化设计 街道分类 街道分类 街道动态活力 街道动态活力
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| GB/T 7714 | 许诺 , 兰思仁 . 基于功能分类和动态活力的街道优化策略研究 [J]. | 建材技术与应用 , 2025 , 6 (01) : 84-89 . |
| MLA | 许诺 et al. "基于功能分类和动态活力的街道优化策略研究" . | 建材技术与应用 6 . 01 (2025) : 84-89 . |
| APA | 许诺 , 兰思仁 . 基于功能分类和动态活力的街道优化策略研究 . | 建材技术与应用 , 2025 , 6 (01) , 84-89 . |
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