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学者姓名：吴祖建
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Abstract ：
Transcription of segmented negative-sense RNA viruses (sNSVs) like rice stripe virus (RSV) is initiated by cap-snatching, where the viral RNA-dependent RNA polymerase cleaves host mRNAs similar to 10-20 nucleotides downstream of the 5 ' cap to generate capped RNA leaders (CRLs) that prime viral mRNA synthesis. We previously demonstrated that purified RSV ribonucleoproteins (RNPs) support transcription in vitro, establishing a host-factor-free system to investigate fundamental aspects of this process. In this study, synthetic capped RNAs (scRNAs) with defined sequences were introduced into this system as CRL donors. Analysis of the resultant RSV transcripts revealed that the minimal system accurately mimics key in vivo features of cap-snatching. Specifically, CRL priming and realignment occurred more frequently on the viral RNA template than on the cRNA template, providing strong evidence that these processes are inherent to the viral RNP complex. Quantitative competition assays further revealed that RSV RNPs exhibit sequence-specific selectivity in cap-snatching, preferentially targeting scRNAs with adenosine or cytidine at positions 11-14, with a discernable bias towards adenosine. Surprisingly, no preference was observed for scRNAs containing AC or CA dinucleotides at the cleavage site, despite their potential to generate CRLs capable of base-pairing over two nucleotides with the viral template. Collectively, these findings offer a deeper understanding of the mechanistic aspects of RSV cap-snatching, which may also inform the study of similar processes in other sNSVs.
Keyword ：
cap-snatching cap-snatching in vitro transcription in vitro transcription prime-and-realign prime-and-realign rice stripe virus rice stripe virus
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| GB/T 7714 | Tian, Liangheng , Lin, Wenzhong , Li, Shuangping et al. Intrinsic features of rice stripe virus cap-snatching revealed by a minimal RNP-based in vitro transcription system [J]. | JOURNAL OF GENERAL VIROLOGY , 2025 , 106 (9) . |
| MLA | Tian, Liangheng et al. "Intrinsic features of rice stripe virus cap-snatching revealed by a minimal RNP-based in vitro transcription system" . | JOURNAL OF GENERAL VIROLOGY 106 . 9 (2025) . |
| APA | Tian, Liangheng , Lin, Wenzhong , Li, Shuangping , Li, Guobin , Lu, Xiaoyu , Wu, Zujian et al. Intrinsic features of rice stripe virus cap-snatching revealed by a minimal RNP-based in vitro transcription system . | JOURNAL OF GENERAL VIROLOGY , 2025 , 106 (9) . |
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Rice viral diseases pose severe threats to global food security, with over 20 viruses identified in China alone. The advent of high-throughput sequencing has accelerated the discovery of novel viruses in cultivated and wild rice, unveiling previously undetected threats. This review systematically summarises newly discovered rice viruses over the past decade, analyzing their genomic characteristics, transmission modes, and pathogenic mechanisms. Key findings include the identification of rice stripe mosaic virus, rice tiller inhibition virus (RTIV), RTIV2 and rice curl dwarf-associated virus, among others, highlighting their interactions with host immunity and insect vectors. Notably, emerging viruses exhibit novel infection strategies, including interference with RNA silencing, hormone signalling, and autophagy pathways. Moreover, mixed infections and cross-species transmission raise concerns about evolving disease dynamics. Understanding these viral threats is crucial for developing integrated disease management strategies, including resistant cultivars and vector control measures. This review provides a comprehensive resource for advancing rice virology research and guiding future efforts in monitoring and mitigating viral diseases affecting global rice production.
Keyword ：
cultivated rice cultivated rice high-throughput sequencing high-throughput sequencing new viruses new viruses wild rice wild rice
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| GB/T 7714 | Ding, Xinlun , Wang, Feng , Liu, Pingping et al. Emerging Viral Threats in Rice: A Decade of Discovery and Implications for Crop Protection [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) : 8702-8711 . |
| MLA | Ding, Xinlun et al. "Emerging Viral Threats in Rice: A Decade of Discovery and Implications for Crop Protection" . | PLANT CELL AND ENVIRONMENT 48 . 12 (2025) : 8702-8711 . |
| APA | Ding, Xinlun , Wang, Feng , Liu, Pingping , Zhang, Jie , Wu, Zujian , Han, Yan-Hong et al. Emerging Viral Threats in Rice: A Decade of Discovery and Implications for Crop Protection . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) , 8702-8711 . |
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Plant viruses often suppress jasmonic acid (JA)-mediated defences through disturbing JA signalling or biosynthesis pathways to benefit their own infection. Few studies have examined how the precursors of JA biosynthesis are regulated by viral infection. In this study, we demonstrate that rice black-streaked dwarf virus (RBSDV) infection inhibits the production of alpha-linolenic acid (C18:3), a key JA biosynthesis precursor that is catalysed by a set of fatty acid desaturases (FADs). The viral capsid protein P10 directly interacts with OsFAD7, an omega-3 fatty acid desaturase, and promotes its autophagic degradation through an ATG8-interaction motif (AIM). This disrupts JA production and weakens antiviral defence against RBSDV infection. Genetic analysis reveals that overexpression of OsFAD7 enhances JA levels and resistance to virus. But OsFAD7-mediated antiviral resistance is attenuated if OsCOI1a, a JA receptor, is silenced, indicating that the enhancement of resistance to RBSDV infection conferred by OsFAD7 depends on the JA pathway. Our findings reveal a novel viral strategy that suppresses JA biosynthesis at its metabolic source, providing insights for developing viral protection strategies and virus-resistant crops.
Keyword ：
antiviral defence antiviral defence autophagy autophagy fatty acids fatty acids jasmonic acid pathway jasmonic acid pathway rice viruses rice viruses
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| GB/T 7714 | Liu, Jianjian , Jing, Xinxin , Wang, Pengyue et al. A fijivirus capsid protein hijacks autophagy degrading an ω-3 fatty acid desaturase to suppress jasmonate-mediated antiviral defence [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (7) : 2891-2907 . |
| MLA | Liu, Jianjian et al. "A fijivirus capsid protein hijacks autophagy degrading an ω-3 fatty acid desaturase to suppress jasmonate-mediated antiviral defence" . | PLANT BIOTECHNOLOGY JOURNAL 23 . 7 (2025) : 2891-2907 . |
| APA | Liu, Jianjian , Jing, Xinxin , Wang, Pengyue , Wang, Gaohua , Xiang, Meirong , Li, Pengbai et al. A fijivirus capsid protein hijacks autophagy degrading an ω-3 fatty acid desaturase to suppress jasmonate-mediated antiviral defence . | PLANT BIOTECHNOLOGY JOURNAL , 2025 , 23 (7) , 2891-2907 . |
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The begomoviral V2 protein is known to be multifunctional, including its interaction with and inhibition of CYP1, a papain-like cysteine protease (PLCP). However, the effect of this interaction on viral pathogenicity remains unclear. Cotton leaf curl Multan virus (CLCuMuV), a typical monopartite begomovirus associated with a betasatellite, is one of the main pathogens responsible for cotton leaf curl disease. This study verifies the interaction between CLCuMuV V2 and NbCP15, a PLCP homologue in Nicotiana benthamiana. The results show that V2 can be cleaved by NbCP15 in vitro, with the N-terminal cleavage site located between the second and third amino acids. Using an Agrobacterium-mediated inoculation method, we investigated the influence of cleavage sites on viral pathogenicity. The findings indicate that mutation of the third amino acid in V2 (V2D3A) reduced the pathogenicity of both heterologous PVX and CLCuMuV. Additionally, the NbCP15 gene mutation in N. benthamiana (nbcp15) also resulted in reduced CLCuMuV pathogenicity. These results suggest that CLCuMuV V2 may promote viral infection through its interaction with plant PLCPs.
Keyword ：
begomovirus begomovirus cotton leaf curl Multan virus cotton leaf curl Multan virus papain-like cysteine protease papain-like cysteine protease V2 protein V2 protein
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| GB/T 7714 | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences [J]. | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
| MLA | Zhang, Jie et al. "Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences" . | MOLECULAR PLANT PATHOLOGY 26 . 1 (2025) . |
| APA | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai , Li, Dingshan , Liu, Shunmin , Du, Zhenguo et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences . | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
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玉蜀黍平脐蠕孢Bipolaris maydis引起的玉米小斑病近年来在福建玉米种植区蔓延为害。为获得对该病害具有良好防效的生防菌株，本研究采用平板稀释法从玉米根际土壤分离拮抗细菌，采用平板对峙法测定不同分离菌株的抑菌活性，筛选拮抗效果良好的菌株进行种类鉴定及抑菌谱和盆栽防效测定。结果表明，从土壤分离获得25株单孢菌株，初筛出3株对玉蜀黍平脐蠕孢具有明显抑制作用的菌株，其中菌株BV-3的抑制带宽最大，达18.04 mm；经形态学、生理生化特征和16S rDNA基因系统发育分析，确认菌株BV-3为贝莱斯芽孢杆菌Bacillus velezensis。该菌株抑菌谱广，对玉米叶斑病5种病原真菌均有良好的抑制作用。盆栽试验结果表明，菌株BV-3发酵液对抗病品种‘新中玉801’和感病品种‘闽甜986’的玉米小斑病7～14 d防效分别达73.72%～92.00%和68.27%～84.62%。可见，贝莱斯芽孢杆菌BV-3可有效防治玉米小斑病，具有良好的生防应用前景。
Keyword ：
玉米小斑病 玉米小斑病 生物防治 生物防治 筛选鉴定 筛选鉴定 贝莱斯芽孢杆菌 贝莱斯芽孢杆菌
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| GB/T 7714 | 吴梦菁 , 黄鹏 , 张杰 et al. 贝莱斯芽孢杆菌BV-3的鉴定及其对玉米小斑病的防效 [J]. | 中国生物防治学报 , 2025 , 41 (04) : 887-894 . |
| MLA | 吴梦菁 et al. "贝莱斯芽孢杆菌BV-3的鉴定及其对玉米小斑病的防效" . | 中国生物防治学报 41 . 04 (2025) : 887-894 . |
| APA | 吴梦菁 , 黄鹏 , 张杰 , 郑璐平 , 余德亿 , 林胜 et al. 贝莱斯芽孢杆菌BV-3的鉴定及其对玉米小斑病的防效 . | 中国生物防治学报 , 2025 , 41 (04) , 887-894 . |
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The genome of a monopartite begomovirus, or the DNA-A component of a bipartite begomovirus, typically encodes six proteins: two on the viral strand (AV1/V1 and AV2/V2) and four on the complementary strand (AC1/ C1, AC2/C2, AC3/C3, AC4/C4). Recent studies, however, have identified additional begomoviral proteins with various functions. This paper reports that euphorbia leaf curl virus (EuLCV), a monopartite begomovirus, encodes a seventh protein, C5. Promoter activity of the upstream fragment of the EuLCV C5 gene was shown using a GUS expression vector. EuLCV C5 also enhanced the pathogenicity and accumulation of potato virus X (PVX) in Nicotiana benthamiana. Localization studies revealed that EuLCV C5 localizes to the cytoplasm and nucleus, forming granular structures on the cell membrane. Additionally, C5 acts as a post-transcriptional gene silencing (PTGS) suppressor. A C5 deletion mutant of EuLCV (EuLCV-Delta C5) exhibited reduced pathogenicity and viral accumulation compared to wild-type EuLCV in N. benthamiana.
Keyword ：
C5 protein C5 protein Euphorbia leaf curl virus Euphorbia leaf curl virus Pathogenicity determinant Pathogenicity determinant Post-transcriptional gene silencing Post-transcriptional gene silencing
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| GB/T 7714 | Zhang, Jie , Yuan, Linkai , Li, Dingshan et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor [J]. | VIROLOGY , 2024 , 600 . |
| MLA | Zhang, Jie et al. "The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor" . | VIROLOGY 600 (2024) . |
| APA | Zhang, Jie , Yuan, Linkai , Li, Dingshan , Yang, Xueying , Li, Jingyuan , Wu, Zujian et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor . | VIROLOGY , 2024 , 600 . |
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Passionfruit (Passiflora edulis) is a significant fruit crop in the commercial sector, owing to its high nutritional and medicinal value. The advent of high-throughput genomics sequencing technology has led to the publication of a vast amount of passionfruit omics data, encompassing complete genome sequences and transcriptome data under diverse stress conditions. To facilitate the efficient integration, storage, and analysis of these large-scale datasets, and to enable researchers to effectively utilize these omics data, we developed the first passionfruit genome database (PGD). The PGD platform comprises a diverse range of functional modules, including a genome browser, search function, heatmap, gene expression patterns, various tools, sequence alignment, and batch download, thereby providing a user-friendly interface. Additionally, supplementary practical tools have been developed for the PGD, such as gene family analysis tools, gene ontology (GO) terms, a pathway enrichment analysis, and other data analysis and mining tools, which enhance the data's utilization value. By leveraging the database's robust scalability, the intention is to continue to collect and integrate passionfruit omics data in the PGD, providing comprehensive and in-depth support for passionfruit research. The PGD is freely accessible via http://passionfruit.com.cn.
Keyword ：
Functional genomics Functional genomics Passiflora edulis Passiflora edulis Passionfruit genomic database Passionfruit genomic database Transcriptomics Transcriptomics Web server Web server
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| GB/T 7714 | Yu, Chaowei , Wang, Peng , Zhang, Shengjie et al. Passionfruit Genomic Database (PGD): a comprehensive resource for passionfruit genomics [J]. | BMC GENOMICS , 2024 , 25 (1) . |
| MLA | Yu, Chaowei et al. "Passionfruit Genomic Database (PGD): a comprehensive resource for passionfruit genomics" . | BMC GENOMICS 25 . 1 (2024) . |
| APA | Yu, Chaowei , Wang, Peng , Zhang, Shengjie , Liu, Jindian , Cheng, Yingyin , Zhang, Songbai et al. Passionfruit Genomic Database (PGD): a comprehensive resource for passionfruit genomics . | BMC GENOMICS , 2024 , 25 (1) . |
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Chinese bayberry (Myrica rubra) is an economically significant fruit tree native to eastern Asia and widely planted in south-central China. However, studies about the viruses infecting M. rubra remain largely lacking. In the present study, we employed the metatranscriptomic method to identify viruses in M. rubra leaves exhibiting yellowing and irregular margin symptoms collected in Fuzhou, a city located in China's Fujian province in the year 2022. As a consequence, a novel member of the genus Totivirus was identified and tentatively named "Myrica rubra associated totivirus 1" (MRaTV1). The genome sequencing of MRaTV1 was determined by overlapping reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The two deduced proteins encoded by MRaTV1 have the highest amino acid (aa) sequence identity to the coat protein (CP) and RNA-dependent RNA polymerase (RdRP) of Panax notoginseng virus A (PNVA), a member of the genus Totivirus within the family Totiviridae, at 49.7% and 61.7%, respectively. According to the results of the phylogenetic tree and the species demarcation criteria of the International Committee on Taxonomy of Viruses (ICTV) for the genus Totivirus, MRaTV1 is considered a new member of the genus Totivirus.
Keyword ：
Myrica rubra Myrica rubra totivirus totivirus virome virome
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| GB/T 7714 | Xu, Zhongtian , Gao, Yi'nan , Teng, Kun et al. Identification and Genome Characterization of a Novel Virus within the Genus Totivirus from Chinese Bayberry (Myrica rubra) [J]. | VIRUSES-BASEL , 2024 , 16 (2) . |
| MLA | Xu, Zhongtian et al. "Identification and Genome Characterization of a Novel Virus within the Genus Totivirus from Chinese Bayberry (Myrica rubra)" . | VIRUSES-BASEL 16 . 2 (2024) . |
| APA | Xu, Zhongtian , Gao, Yi'nan , Teng, Kun , Ge, Huoyang , Zhang, Xiaoqi , Wu, Mengjing et al. Identification and Genome Characterization of a Novel Virus within the Genus Totivirus from Chinese Bayberry (Myrica rubra) . | VIRUSES-BASEL , 2024 , 16 (2) . |
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【目的】建立柑橘黄化脉明病毒（citrus yellow vein clearing virus, CYVCV）、柑橘衰退病毒（citrus tristeza virus, CTV）和啤酒花矮化类病毒（hop stunt viroid, HSVd）的多重RT-PCR检测体系。【方法】设计多重RT-PCR引物，分析其特异性，确定其最佳浓度比、最适退火温度及灵敏度，在此基础上对福建地区的柑橘样品进行检测。【结果】确定了CYVCV-F/R、CTV-F/R和HSVd-F/R等3对引物的最佳浓度比例为1∶1∶2，最适退火温度为52.9℃，灵敏度结果显示该体系可检测模板稀释到10-2的阳性样品。应用该体系对采自福建部分地区的157份柑橘样品进行检测，结果发现，CYVCV、CTV和HSVd的检出率分别为47.1%、56.7%和22.9%。【结论】成功建立了柑橘CYVCV、CTV和HSVd病原的多重RT-PCR检测方法，为该类病害的检测提供准确、快速的检测方法。
Keyword ：
啤酒花矮化类病毒 啤酒花矮化类病毒 多重RT-PCR 多重RT-PCR 柑橘衰退病毒 柑橘衰退病毒 柑橘黄化脉明病毒 柑橘黄化脉明病毒
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| GB/T 7714 | 袁琳凯 , 马崇欢 , 李丁山 et al. 柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用 [J]. | 福建农业学报 , 2024 , 39 (03) : 339-344 . |
| MLA | 袁琳凯 et al. "柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用" . | 福建农业学报 39 . 03 (2024) : 339-344 . |
| APA | 袁琳凯 , 马崇欢 , 李丁山 , 陈志炜 , 江宵烽 , 丁新伦 et al. 柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用 . | 福建农业学报 , 2024 , 39 (03) , 339-344 . |
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Potassium (K+) plays a crucial role as a macronutrient in the growth and development of plants. Studies have definitely determined the vital roles of K+ in response to pathogen invasion. Our previous investigations revealed that rice plants infected with rice grassy stunt virus (RGSV) displayed a reduction in K+ content, but the mechanism by which RGSV infection subverts K+ uptake remains unknown. In this study, we found that overexpression of RGSV P1, a specific viral protein encoded by viral RNA1, results in enhanced sensitivity to low K+ stress and exhibits a significantly lower rate of K+ influx compared to wild-type rice plants. Further investigation revealed that RGSV P1 interacts with OsCIPK23, an upstream regulator of Shaker K+ channel OsAKT1. Moreover, we found that the P1 protein recruits the OsCIPK23 to the Cajal bodies (CBs). In vivo assays demonstrated that the P1 protein competitively binds to OsCIPK23 with both OsCBL1 and OsAKT1. In the nucleus, the P1 protein enhances the binding of OsCIPK23 to OsCoilin, a homologue of the signature protein of CBs in Arabidopsis, and facilitates their trafficking through these CB structures. Genetic analysis indicates that mutant in oscipk23 suppresses RGSV systemic infection. Conversely, osakt1 mutants exhibited increased sensitivity to RGSV infection. These findings suggest that RGSV P1 hinders the absorption of K+ in rice plants by recruiting the OsCIPK23 to the CB structures. This process potentially promotes virus systemic infection but comes at the expense of inhibiting OsAKT1 activity.
Keyword ：
Cajal bodies Cajal bodies OsCIPK23 OsCIPK23 potassium (K+) potassium (K+) RGSV P1 RGSV P1 rice grassy stunt virus (RGSV) rice grassy stunt virus (RGSV)
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| GB/T 7714 | Jing, Xinxin , Wang, Pengyue , Liu, Jianjian et al. A viral protein competitively bound to rice CIPK23 inhibits potassium absorption and facilitates virus systemic infection in rice [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2024 , 22 (8) : 2348-2363 . |
| MLA | Jing, Xinxin et al. "A viral protein competitively bound to rice CIPK23 inhibits potassium absorption and facilitates virus systemic infection in rice" . | PLANT BIOTECHNOLOGY JOURNAL 22 . 8 (2024) : 2348-2363 . |
| APA | Jing, Xinxin , Wang, Pengyue , Liu, Jianjian , Xiang, Meirong , Song, Xia , Wang, Chaonan et al. A viral protein competitively bound to rice CIPK23 inhibits potassium absorption and facilitates virus systemic infection in rice . | PLANT BIOTECHNOLOGY JOURNAL , 2024 , 22 (8) , 2348-2363 . |
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