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学者姓名：郑亚凤
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Abstract ：
Bioactive peptides derived from food proteins have attracted attention for their potential roles in functional foods and pharmaceuticals. Clams, renowned for their protein content and essential amino acids critical to human health, represent a promising source for bioactive peptide production. Recent studies have extensively explored the preparation methods, flavor profiles, and health benefits of clam peptides (CPs). However, there is still a lack of a comprehensive review on the current status of CPs development. This review revealed that enzymatic hydrolysis is the predominant methods for CPs production, which is a potential resource for discovering umami peptides. CPs exhibit diverse bioactivities, including antioxidative, antibacterial, ACE inhibitory, immunomodulatory, and anticancer activities. However, the potential presence of heavy metals, pathogenic bacteria, and allergens in raw materials underscores the need for stringent safety evaluations. In the future, production technology, in vivo fate, health efficacy mechanisms and safety will be interesting directions for CPs research.
Keyword ：
Clam peptide processing Clam peptide processing Clam peptides Clam peptides Health benefits Health benefits Safety risks Safety risks
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| GB/T 7714 | Luo, Xianliang , Pan, Rongbo , Xu, Liping et al. Clam peptides: Preparation, flavor properties, health benefits, and safety risks [J]. | FOOD RESEARCH INTERNATIONAL , 2025 , 207 . |
| MLA | Luo, Xianliang et al. "Clam peptides: Preparation, flavor properties, health benefits, and safety risks" . | FOOD RESEARCH INTERNATIONAL 207 (2025) . |
| APA | Luo, Xianliang , Pan, Rongbo , Xu, Liping , Zheng, Yafeng , Zheng, Baodong . Clam peptides: Preparation, flavor properties, health benefits, and safety risks . | FOOD RESEARCH INTERNATIONAL , 2025 , 207 . |
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This study presents a novel approach for enhancing the survivability of Lactiplantibacillus plantarum BXM2 using bamboo shoot-derived nanocellulose hydrogels. Nanocellulose hydrogels, composed of cellulose nanofibers (CNFs), cellulose nanocrystals (CNCs), and polyvinyl alcohol (PVA), were developed as protective matrices for probiotics. Fourier transform infrared spectroscopy (FT-IR) and X-ray diffraction (XRD) confirmed the successful formation of hydrogen-bonded networks between PVA and nanocelluloses, while scanning electron microscopy (SEM) revealed that the ternary PVA-CNF-CNC hydrogel exhibited a dense, hierarchical porous structure, effectively encapsulating probiotics with an encapsulation efficiency of 92.56 +/- 0.53%. Under simulated gastrointestinal digestion, the encapsulated probiotics maintained 8.04 log CFU/g viability, significantly higher than that of free bacteria (3.54 log CFU/mL). The hydrogel also enhanced heat tolerance (6.58 log CFU/mL at 70 degrees C) and freeze-drying survival (86.92% viability), outperforming binary systems. During 60-day storage at 4 degrees C and 25 degrees C, encapsulated probiotics retained viability above the critical threshold (>= 6 log CFU/unit), whereas free cells declined rapidly. These findings highlight the potential of PVA-CNF-CNC hydrogel as an efficient delivery system to improve probiotic stability in food applications.
Keyword ：
hydrogel hydrogel nanocellulose nanocellulose probiotics probiotics viability viability
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| GB/T 7714 | Huang, Yajuan , Guan, Qiao , Wu, Yirui et al. Microencapsulation of Lactiplantibacillus plantarum BXM2 in Bamboo Shoot-Derived Nanocellulose Hydrogel to Enhance Its Survivability [J]. | GELS , 2025 , 11 (6) . |
| MLA | Huang, Yajuan et al. "Microencapsulation of Lactiplantibacillus plantarum BXM2 in Bamboo Shoot-Derived Nanocellulose Hydrogel to Enhance Its Survivability" . | GELS 11 . 6 (2025) . |
| APA | Huang, Yajuan , Guan, Qiao , Wu, Yirui , Zheng, Chaoyang , Zhong, Lingyue , Xie, Wen et al. Microencapsulation of Lactiplantibacillus plantarum BXM2 in Bamboo Shoot-Derived Nanocellulose Hydrogel to Enhance Its Survivability . | GELS , 2025 , 11 (6) . |
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本发明涉及水产品加工技术领域，具体涉及一种抑制鱼糜贮藏过程中蛋白冷冻变性的方法，其包括如下步骤：向鱼糜中加入1‑5%质量百分数的纤维素纳米晶体得鱼糜样品；在0‑10°C环境中将鱼糜样品混合均匀，并加蒸馏水将所述鱼糜样品水分控制在质量百分数为75‑85%。本发明的有益效果在于：本发明以天然膳食纤维CNC为外源添加剂添加至鱼糜中，发现其对冰晶行为具有良好的调控作用，在鱼糜中使用可起到良好地抑制鱼糜结构破坏与持水能力下降。本发明制备过程简单，原料来源广泛、性能稳定，为开发新型绿色健康的鱼糜冷冻保护剂提供数据支撑，在食品领域具有广阔的应用空间。
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| GB/T 7714 | 郑亚凤 , 倪乾乾 , 杜艳瑜 et al. 一种抑制鱼糜贮藏过程中蛋白冷冻变性的方法 : CN202411802858.X[P]. | 2024-12-09 . |
| MLA | 郑亚凤 et al. "一种抑制鱼糜贮藏过程中蛋白冷冻变性的方法" : CN202411802858.X. | 2024-12-09 . |
| APA | 郑亚凤 , 倪乾乾 , 杜艳瑜 , 关乔 , 庄玮婧 , 黄雅涓 et al. 一种抑制鱼糜贮藏过程中蛋白冷冻变性的方法 : CN202411802858.X. | 2024-12-09 . |
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本发明涉及微生物技术领域，具体涉及一种益生菌微胶囊及其制备方法。所述制备方法包括如下步骤：制备益生菌的菌悬液；将聚乙烯醇溶液，纳米纤维素和纳米纤维素晶体混合水浴加热至完全溶解配制成PCC悬浮液；将所得PCC悬浮液高压灭菌，得纳米纤维素基水凝胶微胶囊；将所述纳米纤维素基水凝胶微胶囊与菌悬液混合，得到益生菌微胶囊。本发明的有益效果在于：纳米纤维素纤维、纳米纤维素晶体与聚乙烯醇相结合形成致密的网络结构可有效增强益生菌在恶劣环境下的稳定性和活性。进一步的，制备的纳米纤维素微胶囊包封益生菌，可提高益生菌在冷冻干燥和加热条件下的存活率。
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| GB/T 7714 | 郑亚凤 , 黄雅涓 , 关乔 et al. 一种益生菌微胶囊及其制备方法 : CN202411802857.5[P]. | 2024-12-09 . |
| MLA | 郑亚凤 et al. "一种益生菌微胶囊及其制备方法" : CN202411802857.5. | 2024-12-09 . |
| APA | 郑亚凤 , 黄雅涓 , 关乔 , 杜艳瑜 , 庄玮婧 , 余明雨 et al. 一种益生菌微胶囊及其制备方法 : CN202411802857.5. | 2024-12-09 . |
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Objective: Lactiplantibacillus plantarum BXM2 is a novel probiotic derived from fermented passion fruit (Passiflora edulis) juice that possesses promising probiotic potential. The aim of this study was to evaluate the beneficial effects of L. plantarum BXM2 supplementation in mice. Methods: L. plantarum BXM2 was orally administered to male SPF C57BL/6J mice fed a high-fat diet (HFD) to evaluate its anti-obesity potential, as well as the effects on intestinal health and microbiota. Results: Our results demonstrated that L. plantarum BXM2 significantly decreased the perirenal adipose index and improved intestinal health by increasing the ratio of villus height to crypt depth and the goblet cell number in the intestine. Furthermore, L. plantarum BXM2 treatment exhibited regulatory effects on intestinal chronic inflammation in mice by normalizing the mRNA expression of TNF-alpha and IL-6. Of note, L. plantarum BXM2 reversed HFD-induced gut dysbiosis, as evidenced by the decreased ratio of Bacillota (Firmicutes) to Bacteroidota, the decreased abundance of obesity-related genera Dubosiella, Romboutsia, and Lachnospiraceae_UCG006, and the increased abundance of beneficial genera Akkermansia and Lactobacillus. Conclusions: Our findings support the beneficial role of L. plantarum BXM2 supplementation in interventions targeting gut dysbiosis and obesity-related disorders.
Keyword ：
cytokines cytokines gut microbiota gut microbiota Lactiplantibacillus plantarum BXM2 Lactiplantibacillus plantarum BXM2 obesity obesity probiotic probiotic
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| GB/T 7714 | Cai, Xiaohui , Huang, Juqing , Yu, Tian et al. Lactiplantibacillus plantarum BXM2 Treatment Alleviates Disorders Induced by a High-Fat Diet in Mice by Improving Intestinal Health and Modulating the Gut Microbiota [J]. | NUTRIENTS , 2025 , 17 (3) . |
| MLA | Cai, Xiaohui et al. "Lactiplantibacillus plantarum BXM2 Treatment Alleviates Disorders Induced by a High-Fat Diet in Mice by Improving Intestinal Health and Modulating the Gut Microbiota" . | NUTRIENTS 17 . 3 (2025) . |
| APA | Cai, Xiaohui , Huang, Juqing , Yu, Tian , Guan, Xuefang , Sun, Meng , Zhao, Dazhou et al. Lactiplantibacillus plantarum BXM2 Treatment Alleviates Disorders Induced by a High-Fat Diet in Mice by Improving Intestinal Health and Modulating the Gut Microbiota . | NUTRIENTS , 2025 , 17 (3) . |
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This study was to investigate the fundamental composition and structure, hypoglycemic activity and fermentation characteristics of Porphyra haitanensis glycoprotein (PG) following in vitro simulate gastrointestinal digestion. After in vitro digestion, PG was degraded into peptides, amino acids, and reducing sugars, and the digested PG structure evolved into functional aggregates that were more favorable for regulating intestinal flora. Such aggregated structure might inhibit the accessibility of digestive enzymes and significantly enhanced its hypoglycemic activity. Compared with undigested PG, the inhibition rate of digested PG on alpha-amylase and alpha-glucosidase was increased by 4.70 % and 7.69 %, respectively (P < 0.05). Furthermore, digested PG enhanced the abundances of Lactobacillus and Bifidobacterium, etc., which was positive related with the increased secretion of butyric acid, thereby playing a role in regulating intestinal homeostasis and blood glucose homeostasis in the host. Our findings can offer novel perspectives on the regulation of the intestinal flora by glycoproteins during gastrointestinal digestion.
Keyword ：
Fermentation characteristics Fermentation characteristics Hypoglycemic activity Hypoglycemic activity In vitro digestion In vitro digestion Porphyra haitanensis glycoprotein Porphyra haitanensis glycoprotein Structural evolution Structural evolution
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| GB/T 7714 | Ou, Yujia , Xie, Wen , Wang, Haoyu et al. In vitro digestion of glycoproteins from Porphyra haitanensis: Effects on structure, hypoglycemic activity, and fermentation characteristics [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 289 . |
| MLA | Ou, Yujia et al. "In vitro digestion of glycoproteins from Porphyra haitanensis: Effects on structure, hypoglycemic activity, and fermentation characteristics" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 289 (2025) . |
| APA | Ou, Yujia , Xie, Wen , Wang, Haoyu , Zheng, Yafeng , Guo, Zebin , Zheng, Baodong . In vitro digestion of glycoproteins from Porphyra haitanensis: Effects on structure, hypoglycemic activity, and fermentation characteristics . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 289 . |
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To tackle the poor emulsibility of hydrophilic cellulose nanocrystals (CNCs), this study prepared octenyl succinic anhydride (OSA)-modified bamboo shoot CNC (OSNC) via acid hydrolysis and esterification, using microcrystalline cellulose (MCC) as a control. The degree of substitution (DS), chemical structure, crystalline structure, morphological characteristics, zeta potential, wettability, and thermal stability of OSNC and OSA-modified MCC (OSA-MCC) were characterized using multiple techniques. Results showed that at the optimal cellulose-to-OSA ratio (1:0.225), OSNC had a higher DS (0.029 +/- 0.01) than OSA-MCC (0.024 +/- 0.02). FTIR confirmed successful OSA grafting; XRD showed a preserved cellulose I crystal form with slightly reduced crystallinity; OSNC had improved dispersion stability (zeta potential: -44.0 mV), balanced amphiphilicity (contact angle: 61.8 degrees), and enhanced thermal stability. This work enables high-value utilization of bamboo shoot by-products and supports developing green food-grade cellulose-based nanomaterials for food emulsions.
Keyword ：
bamboo shoot by-products bamboo shoot by-products cellulose nanocrystals (CNCs) cellulose nanocrystals (CNCs) emulsion stabilizer emulsion stabilizer octenyl succinic anhydride (OSA) modification octenyl succinic anhydride (OSA) modification
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| GB/T 7714 | Huang, Maokun , Chen, Wen , Zhang, Zichen et al. Preparation, Structural Characterization of Octenyl Succinic Anhydride-Modified Bamboo Shoot-Derived Cellulose Nano-Crystals [J]. | FOODS , 2025 , 14 (22) . |
| MLA | Huang, Maokun et al. "Preparation, Structural Characterization of Octenyl Succinic Anhydride-Modified Bamboo Shoot-Derived Cellulose Nano-Crystals" . | FOODS 14 . 22 (2025) . |
| APA | Huang, Maokun , Chen, Wen , Zhang, Zichen , Wang, Qi , Li, Yunlong , Zheng, Yafeng . Preparation, Structural Characterization of Octenyl Succinic Anhydride-Modified Bamboo Shoot-Derived Cellulose Nano-Crystals . | FOODS , 2025 , 14 (22) . |
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The preparation of cellulose nanofiber (CNF) using traditional methods is currently facing challenges due to concerns regarding environmental pollution and safety. Herein, a novel CNF was obtained from bamboo shoot shell (BSS) by low-concentration acid and dynamic high-pressure microfluidization (DHPM) treatment. The resulting CNF was then characterized, followed by in vitro and in vivo safety assessments. Compared to insoluble dietary fiber (IDF), the diameters of HIDF (IDF after low-concentration acid hydrolysis) and CNF were significantly decreased to 167.13 nm and 70.97 nm, respectively. Meanwhile, HIDF and CNF showed a higher crystallinity index (71.32 % and 74.35 %). Structural analysis results indicated the successful removal of lignin and hemicellulose of HIDF and CNF, with CNF demonstrating improved thermostability. In vitro, a high dose of CNF (1500 mu g/mL) did not show any signs of cytotoxicity on Caco-2 cells. In vivo, no death was observed in the experimental mice, and there was no significant difference between CNF (1000 mg/kg & sdot;bw) and control group in hematological index and histopathological analysis. Overall, this study presents an environmentally friendly method for preparing CNF from BSS while providing evidence regarding its safety through in vitro and in vivo assessments, laying the foundation for its potential application in food.
Keyword ：
Bamboo shoot shell Bamboo shoot shell Cellulose nanofiber Cellulose nanofiber Characterization Characterization Toxicology Toxicology
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| GB/T 7714 | Luo, Xianliang , Wang, Qi , Liu, Wangxin et al. Characterization and safety assessment of bamboo shoot shell cellulose nanofiber: Prepared by acidolysis combined with dynamic high-pressure microfluidization [J]. | CARBOHYDRATE POLYMERS , 2024 , 335 . |
| MLA | Luo, Xianliang et al. "Characterization and safety assessment of bamboo shoot shell cellulose nanofiber: Prepared by acidolysis combined with dynamic high-pressure microfluidization" . | CARBOHYDRATE POLYMERS 335 (2024) . |
| APA | Luo, Xianliang , Wang, Qi , Liu, Wangxin , Wu, Yirui , Yang, Junyi , Chen, Peng et al. Characterization and safety assessment of bamboo shoot shell cellulose nanofiber: Prepared by acidolysis combined with dynamic high-pressure microfluidization . | CARBOHYDRATE POLYMERS , 2024 , 335 . |
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Immunomodulatory effects of lotus seed (Nelumbo nucifera Gaertn.) peptides (LSPs) were studied in this paper. The LSPs were prepared by trypsin enzymatic hydrolysis and were separated by ultrafiltration separation technology. The following four peptide fractions with different molecular weight were obtained: LSP-I (>10 kDa), LSP-II (5-10 kDa), LSP-III (3-5 kDa), and LSP-IV (<3 kDa). Macrophage phagocytosis capacity, NO production and cytokine levels were measured in healthy and immunosuppressed mice to explore the effects of immunomodulatory activity of LSPs. In vitro, among the four peptides, LSP-IV showed the greatest effect on RAW264.7 macrophage phagocytosis. In vivo, LSP-IV had no significant effect on the levels of nitric oxide (NO), interleukin 6 (IL-6) and tumor necrosis factor (TNF-alpha) in healthy mice in a dose-dependent manner, but significantly increased the levels of NO, IL -6 and TNF-alpha secreted by peritoneal macrophages (p < 0.01 and p < 0.001, respectively) in immunosuppressed mice. In particular, high doses of LSP-IV (0.10 g/kg body weight) showed a stronger immunomodulatory potential. Our findings shed light on the potential of low-molecular-weight peptides from lotus seeds as novel natural immunoregulatory dietary factors.
Keyword ：
Cytokines Cytokines Immunomodulatory effects Immunomodulatory effects Lotus seed(Nelumbo nuciferaGaertn.) peptides Lotus seed(Nelumbo nuciferaGaertn.) peptides Macrophages Macrophages
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| GB/T 7714 | Zhuang, Weijing , Lu, Xu , Zheng, Yafeng et al. Immunomodulatory effects of lotus seed (Nelumbo nucifera Gaertn.) peptides on macrophages in mice [J]. | FOOD BIOSCIENCE , 2024 , 57 . |
| MLA | Zhuang, Weijing et al. "Immunomodulatory effects of lotus seed (Nelumbo nucifera Gaertn.) peptides on macrophages in mice" . | FOOD BIOSCIENCE 57 (2024) . |
| APA | Zhuang, Weijing , Lu, Xu , Zheng, Yafeng , Song, Hongbo , Zheng, Baodong . Immunomodulatory effects of lotus seed (Nelumbo nucifera Gaertn.) peptides on macrophages in mice . | FOOD BIOSCIENCE , 2024 , 57 . |
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Phosphorylation is a key route to achieve varieties of biological activities for polysaccharides. Here, we report the phosphorylated surface deacetylated chitin nanofibers (PS-ChNFs) using the sodium tripolyphosphate/so-dium trimetaphosphate (STPP/STMP) method. Response surface methodology (RSM) was employed to optimize in this study. Under optimal conditions, a maximum degree of substitution (DS) of 0.13 was obtained. In addition, the structures of PS-ChNFs were investigated by Fourier transform infrared spectroscopy (FT-IR), Nuclear Magnetic Resonance spectra (NMR), X-ray photoelectron spectroscopy (XPS), Scanning electron mi-croscope (SEM) and (Energy Dispersive Spectroscopy-mapping) EDS-mapping. The findings revealed that the FT -IR spectroscopy and XPS analysis confirmed the appearance of phosphate groups in PS-ChNFs. The 31P NMR results indicate that the PS-ChNFs structure has characteristic peaks of P elements. SEM images showed that PS-ChNFs had a rough surface with many cavities, but the P elements on the surface of the EDS-mapping are uniformly distributed throughout the sample without any enrichment. Antioxidant and antibacterial test showed that PS-ChNFs had significant scavenging effect on free radicals and antibacterial effect. The above results indicate that the chemical modification of PS-ChNFs was successful.
Keyword ：
Antioxidant and antibacterial test Antioxidant and antibacterial test Phosphorylation Phosphorylation Structures Structures
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| GB/T 7714 | Yu, Shan , Duan, Mengxia , Zeng, Ronghuai et al. Preparation, characterization and biological activity of phosphorylated surface deacetylated chitin nanofibers [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2023 , 233 . |
| MLA | Yu, Shan et al. "Preparation, characterization and biological activity of phosphorylated surface deacetylated chitin nanofibers" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 233 (2023) . |
| APA | Yu, Shan , Duan, Mengxia , Zeng, Ronghuai , Chen, Fujie , Zhong, Weiquan , Sun, Jishuai et al. Preparation, characterization and biological activity of phosphorylated surface deacetylated chitin nanofibers . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2023 , 233 . |
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