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学者姓名：李毓
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A spontaneous barley mutant, young leaf chlorina (ylc), was identified in this study. Young leaves of ylc exhibited a yellow base and green tip, with reduced chlorophyll content, and altered chlorophyll fluorescence pattern, and underdeveloped grana in chloroplasts. The color of mutant leaves gradually transitioned to pale green and then became green in mature leaves. The ylc phenotype was found to be controlled by a recessive locus mapped to a 2.4 Mb interval on chromosome 7HS using bulked-segregant analysis with deep sequencing and further fine mapped to a 410 kb interval using polymorphic markers. The YLC locus co-segregated with a molecular marker that led to identification of HORVU7Hr1G011570 as the most likely candidate gene. As compared to the barley reference genome, the candidate ylc allele contained nucleotide changes that would lead to functional alterations of its protein product. The candidate YLC gene encodes a DYW-type pentatricopeptide repeat (PPR) protein, implicated in RNA cleavage and RNA editing in chloroplasts. Chlorophyll fluorescence analysis suggests that the PPR protein may regulate chloroplast development through the function of NAD(P)H dehydrogenase (NDH) complex and plays a pivotal role in mediating electron flow in thylakoid membranes during leaf growth in barley.
Keyword ：
Barley Barley BSA-seq BSA-seq Fine mapping Fine mapping PPR PPR RNA-seq RNA-seq Young leaf chlorina Young leaf chlorina
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| GB/T 7714 | Huang, Biguang , Huang, Daiqing , Zhang, Jianchun et al. Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves [J]. | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
| MLA | Huang, Biguang et al. "Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves" . | PLANT MOLECULAR BIOLOGY 115 . 2 (2025) . |
| APA | Huang, Biguang , Huang, Daiqing , Zhang, Jianchun , Xiong, Jiwei , Wu, Shiyu , Zheng, Xinrong et al. Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves . | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
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Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism, we reconstructed a mapping segregation population (BC1F1) of 12 000 plants. Using the population and polymorphism of simple sequence repeat (SSR) molecular markers, we finally mapped Rf(fa) between the two SSR molecular markers MM2000 and RM25658, within a 78.87 kb region. By de novo sequencing of a resto-ring line of CMS-FA hybrid rice, we obtained the genomic sequence of the mapping region, which provided the basis for the prediction of the candidate gene(s) of the target gene and for the comparison of genomic sequence differences between wild and cultivated rice. Within the mapping region, the genomic sequence of the wild rice was significantly different from that of cultivated rice. There were ten genes in the final mapping region. A pentatricopeptide repeat (PPR) protein gene was predicted as the candidate gene of Rf(fa). Our results laid a solid foundation for the final cloning and molecular mechanism analysis of the gene. The identified molecular markers tightly linked to Rf(fa) will facilitate the marker assisted selection in breeding of CMS-FA hybrid rice.
Keyword ：
fertility restorer fertility restorer gene fine mapping gene fine mapping prediction of candidate gene(s) prediction of candidate gene(s) rice rice
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| GB/T 7714 | Li, Yu , Chen, Xiaomi , Lan, Tao et al. Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice [J]. | CZECH JOURNAL OF GENETICS AND PLANT BREEDING , 2022 , 59 (1) : 43-53 . |
| MLA | Li, Yu et al. "Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice" . | CZECH JOURNAL OF GENETICS AND PLANT BREEDING 59 . 1 (2022) : 43-53 . |
| APA | Li, Yu , Chen, Xiaomi , Lan, Tao , Zhang, Jing , Chen, Ziheng , Yang, Wenting et al. Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice . | CZECH JOURNAL OF GENETICS AND PLANT BREEDING , 2022 , 59 (1) , 43-53 . |
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High oil and protein content make tetraploid peanut a leading oil and food legume. Here we report a high-quality peanut genome sequence, comprising 2.54 Gb with 20 pseudomolecules and 83,709 protein-coding gene models. We characterize gene functional groups implicated in seed size evolution, seed oil content, disease resistance and symbiotic nitrogen fixation. The peanut B subgenome has more genes and general expression dominance, temporally associated with long-terminal-repeat expansion in the A subgenome that also raises questions about the A-genome progenitor. The polyploid genome provided insights into the evolution of Arachis hypogaea and other legume chromosomes. Resequencing of 52 accessions suggests that independent domestications formed peanut ecotypes. Whereas 0.42-0.47 million years ago (Ma) polyploidy constrained genetic variation, the peanut genome sequence aids mapping and candidate-gene discovery for traits such as seed size and color, foliar disease resistance and others, also providing a cornerstone for functional genomics and peanut improvement.
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| GB/T 7714 | Zhuang, Weijian , Chen, Hua , Yang, Meng et al. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication [J]. | NATURE GENETICS , 2019 , 51 (5) : 865-, . |
| MLA | Zhuang, Weijian et al. "The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication" . | NATURE GENETICS 51 . 5 (2019) : 865-, . |
| APA | Zhuang, Weijian , Chen, Hua , Yang, Meng , Wang, Jianping , Pandey, Manish K. , Zhang, Chong et al. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication . | NATURE GENETICS , 2019 , 51 (5) , 865-, . |
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脱落酸(ABA)在植物生长发育和逆境胁迫响应等过程中具有重要作用。脱落酸8'-羟化酶是ABA分解及其代谢的关键酶,可降解内源ABA水平。本研究利用Reverse Transcription PCR(RT-PCR)的方法从花生品种闽花6号叶片中克隆了脱落酸8'-羟化酶基因AhCYP707A4的开放阅读框(Open Reading Frame,ORF)。AhCYP707A4基因ORF长1 446 base pairs(bp),编码481个氨基酸。生物信息学分析表明,该蛋白N端有一个长32个氨基酸残基的信号肽序列,其后为P450蛋白保守区,符合典型的P450蛋白的结构特征。氨基酸多序列比对显示AhCYP707A4具有较高的保守序列。实时荧光定量PCR分析表明,该基因在不同发育时期的果皮、种皮和胚中均有表达。在果皮和种皮中,该基因在花生果针入土后40 d(days after pegging,DAP)表达量最高,而胚中在60 d中表达量达到最高。AhCYP707A4基因可能在花生发育过程起重要的调控作用,是调控花生种子发育特别是胚发育的重要候选基因。
Keyword ：
基因克隆 基因克隆 胚发育 胚发育 脱落酸8'-羟化酶基因 脱落酸8'-羟化酶基因 花生 花生 表达分析 表达分析
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| GB/T 7714 | 康苗苗 , 张冲 , 庄瑞蓉 et al. 花生脱落酸8'-羟化酶基因AhCYP707A4的克隆及表达分析 [J]. | 分子植物育种 , 2016 , 14 (04) : 812-818 . |
| MLA | 康苗苗 et al. "花生脱落酸8'-羟化酶基因AhCYP707A4的克隆及表达分析" . | 分子植物育种 14 . 04 (2016) : 812-818 . |
| APA | 康苗苗 , 张冲 , 庄瑞蓉 , 李毓 , 陈华 . 花生脱落酸8'-羟化酶基因AhCYP707A4的克隆及表达分析 . | 分子植物育种 , 2016 , 14 (04) , 812-818 . |
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对农林院校《种子检验学》实验教学中关于种子质量检验实验所涉及系列问题,包括植物材料、分子标记、DNA分离、PCR条件、凝胶制备与电泳等方面问题进行了研究与探讨,提出了具体可行的实验方案,使分子标记技术适用于实验课堂教学操作应用,供从事相关实验的教师参考。
Keyword ：
SSR SSR 分子标记 分子标记 电泳 电泳 种子质量检验 种子质量检验
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| GB/T 7714 | 李毓 , 黄琴 , 寇贺玲 et al. SSR分子标记技术在农林院校《种子检验学》实验教学中的应用 [J]. | 教育现代化 , 2016 , 3 (36) : 141-143 . |
| MLA | 李毓 et al. "SSR分子标记技术在农林院校《种子检验学》实验教学中的应用" . | 教育现代化 3 . 36 (2016) : 141-143 . |
| APA | 李毓 , 黄琴 , 寇贺玲 , 兰涛 , 章清杞 , 赵春慧 . SSR分子标记技术在农林院校《种子检验学》实验教学中的应用 . | 教育现代化 , 2016 , 3 (36) , 141-143 . |
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The Fujian Abortion cytoplasmic male sterility (CMS-FA) system, a new type of sporophytic CMS system in indica rice (Oryza sativa L.), was developed using the cytoplasm and the corresponding fertility-restoring gene from a wild rice (O. rufipogon L.), which originated from Fujian Province, China. Previous studies in combination with several years of production practice demonstrated that CMS-FA hybrid rice was superior to CMS-WA hybrid rice, a prevailing hybrid rice worldwide, and that the male fertility restoration was controlled by a pair of dominant alleles. We tentatively designated the fertility restoration gene as Rf(fa). The analysis of the polymorphism between the fertile and sterile pool DNAs from a mapping segregation population (BC1F1) indicated that Rf(fa) was located on rice chromosome 10. We further delimited the Rf(fa) locus to a 121.1-kb region flanked by RM6100 and MM2023, which were approximately 0.26 cM and 0.18 cM away from Rf(fa), respectively, by simple sequence repeat molecular marker linkage genetic analysis. These results would facilitate the map-based cloning of Rf(fa), the elucidation of a novel molecular mechanism underlying cytoplasm-nucleus interaction in the CMS-FA system, and the production application of this hybrid rice.
Keyword ：
CMS-FA CMS-FA Fertility restoration gene Fertility restoration gene Fine mapping Fine mapping Linkage genetic analysis Linkage genetic analysis Rice Rice
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| GB/T 7714 | Li, Yu , Zhang, Min , Yang, Xiaofei et al. Fine mapping of a fertility restoring gene for a new CMS hybrid rice system [J]. | MOLECULAR BREEDING , 2016 , 36 (10) . |
| MLA | Li, Yu et al. "Fine mapping of a fertility restoring gene for a new CMS hybrid rice system" . | MOLECULAR BREEDING 36 . 10 (2016) . |
| APA | Li, Yu , Zhang, Min , Yang, Xiaofei , Lin, Chunfang , Duan, Yuanlin , Wang, Naiyuan . Fine mapping of a fertility restoring gene for a new CMS hybrid rice system . | MOLECULAR BREEDING , 2016 , 36 (10) . |
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Yellow leaf of sugarcane is caused by Sugarcane yellow leaf virus (SCYLV) and has been a threat to global sugar industry. Understanding the diversity and genetic structure of plant virus populations may help to develop strategies to control virus-induced diseases. In this study, demography, genetic differentiation and selection pressure were assessed based on 57 nucleotide sequences of the P0 (567 nt) and 54 nucleotide sequences of the P1 (1413 nt) genes of SCYLV isolates. Phylogenetic analysis revealed three phylogeographical groups: African (Clade I), Chinese/American (Clade II), and Asian (Clade III). Genetic distance values between groups (0.034-0.250) were higher than within-group values (0.011-0.033). The African group was in a state of expansion, with lower nucleotide sequence diversity. Except for those isolates within the Asian group, SCYLV showed infrequent gene flow within or between phylogenetic groups. Analysis of selection pressure suggested that these two genes have been subjected to negative (purifying) selection. However, some sites, especially in the P0 coding region of the Chinese/American group, have undergone positive selection. Overall, aside from recombination and founder effects, gene flow and purifying selection are two important factors driving SCYLV evolution.
Keyword ：
Gene flow Gene flow Genetic variability Genetic variability Population structure Population structure Selection pressure Selection pressure Sugarcane yellow leaf virus Sugarcane yellow leaf virus
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| GB/T 7714 | Zhao, Chun-Hui , Lin, Yi-Hua , Pan, Yong-Bao et al. Population structure and selection pressure analysis among Sugarcane yellow leaf virus isolates based on P0 and P1 sequences [J]. | TROPICAL PLANT PATHOLOGY , 2016 , 41 (4) : 237-245 . |
| MLA | Zhao, Chun-Hui et al. "Population structure and selection pressure analysis among Sugarcane yellow leaf virus isolates based on P0 and P1 sequences" . | TROPICAL PLANT PATHOLOGY 41 . 4 (2016) : 237-245 . |
| APA | Zhao, Chun-Hui , Lin, Yi-Hua , Pan, Yong-Bao , Fu, Hua-Ying , Chen, Ru-Kai , Grisham, Michael Paul et al. Population structure and selection pressure analysis among Sugarcane yellow leaf virus isolates based on P0 and P1 sequences . | TROPICAL PLANT PATHOLOGY , 2016 , 41 (4) , 237-245 . |
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育成新质源雄性不育系金农2A和恢复系金恢3号,组配成杂交稻金农2优3号,2007-2008年参加福建省晚稻区域试验,2009年参加生产试验,主要技术指标:(1)产量.福建省2007年晚稻区域试验,比对照汕优63增产9.07%.2008年继续试验比对照汕优63增产8.98%,两年平均比对照增产9.03%.2009年生产试验比对照汕优63增产16.49%.(2)稻米品质.稻米品质达到部颁三等优质食用米的品质标准,直链淀粉含量15.70%.(3)抗病性.中感稻瘟病.(4)全生育期.比汕优63迟熟2.3 d.
Keyword ：
创新育种 创新育种 新质源杂交稻 新质源杂交稻 选育 选育 金农2优3号 金农2优3号
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| GB/T 7714 | 王乃元 , 李毓 , 王颖 et al. 新质源(CMS-FA)杂交稻组合金农2优3号选育 [J]. | 福建农林大学学报(自然科学版) , 2014 , 43 (04) : 345-349 . |
| MLA | 王乃元 et al. "新质源(CMS-FA)杂交稻组合金农2优3号选育" . | 福建农林大学学报(自然科学版) 43 . 04 (2014) : 345-349 . |
| APA | 王乃元 , 李毓 , 王颖 , 陈爱媚 , 梁康迳 , 张敏 et al. 新质源(CMS-FA)杂交稻组合金农2优3号选育 . | 福建农林大学学报(自然科学版) , 2014 , 43 (04) , 345-349 . |
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利用4个CMS-FA胞质不育系、相应保持系和3个恢复系,研究不育细胞对11个产量相关性状的细胞质效应和核质互作效应.结果表明:CMS-FA胞质杂交稻不育细胞质对千粒重和生育期性状表现出显著正效应,对穗颈长性状表现出显著负效应,其他性状的不育细胞质效应不显著;单株穗数、千粒重、每穗总粒数、结实率、株高,穗长、穗颈长和生育期等性状的核质互作效应均达到了显著或极显著水平;不育胞质与核基因的互作使杂种F1的平均基因型预测值、群体平均优势和群体超亲优势在株高和穗颈长上得到明显增加;CMS-FA胞质杂交稻不育细胞质无不良细胞质效应,不育系可恢复性好,可作为杂交稻育种的优良不育胞质加以利用.
Keyword ：
CMS-FA CMS-FA 产量相关性状 产量相关性状 杂交稻 杂交稻 核质互作 核质互作 细胞质效应 细胞质效应
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| GB/T 7714 | 仇秀丽 , 王洪飞 , 王乃元 et al. CMS-FA胞质杂交稻不育细胞质和核质互作的遗传效应分析 [J]. | 福建农林大学学报(自然科学版) , 2013 , 42 (02) : 113-118 . |
| MLA | 仇秀丽 et al. "CMS-FA胞质杂交稻不育细胞质和核质互作的遗传效应分析" . | 福建农林大学学报(自然科学版) 42 . 02 (2013) : 113-118 . |
| APA | 仇秀丽 , 王洪飞 , 王乃元 , 李毓 , 梁康迳 . CMS-FA胞质杂交稻不育细胞质和核质互作的遗传效应分析 . | 福建农林大学学报(自然科学版) , 2013 , 42 (02) , 113-118 . |
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利用已公布的水稻Cry1a与Cry1b基因序列设计引物,PCR扩增基因部分片段,构建了一个包含2个隐花色素基因片段的ihpRNA表达载体pSC1301-347-Cry1aCry1b,并通过农杆菌介导转化水稻,以期同时导致这2个基因功能缺失.根据转基因植株主要农艺性状的表现,初步分析了Cry1a与Cry1b对水稻重要农艺性状的影响.结果表明,转基因水稻开花期推迟16 d,株高增加明显,粒型显著变长,而其他所观察的性状无明显改变.
Keyword ：
Cry1a/Cry1b基因 Cry1a/Cry1b基因 ihpRNA ihpRNA 农艺性状 农艺性状 水稻 水稻 隐花色素 隐花色素
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| GB/T 7714 | 李毓 , 庄伟建 , 庄春红 et al. 干扰隐花色素Cry1a与Cry1b基因的表达对水稻若干农艺性状的影响 [J]. | 福建农林大学学报(自然科学版) , 2012 , 41 (02) : 153-158 . |
| MLA | 李毓 et al. "干扰隐花色素Cry1a与Cry1b基因的表达对水稻若干农艺性状的影响" . | 福建农林大学学报(自然科学版) 41 . 02 (2012) : 153-158 . |
| APA | 李毓 , 庄伟建 , 庄春红 , 王乃元 , 戴飞 . 干扰隐花色素Cry1a与Cry1b基因的表达对水稻若干农艺性状的影响 . | 福建农林大学学报(自然科学版) , 2012 , 41 (02) , 153-158 . |
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