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Stepwise deactivation of gibberellins during rice internode elongation SCIE
期刊论文 | 2025 , 122 (23) | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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Abstract :

Bioactive gibberellins (GAs) are a class of plant hormones that regulate various aspects of plant growth and development, and several key GA deactivation enzymes have been identified. In rice, non- 13- hydroxylated GAs have been shown to be deactivated via 160x,17- epoxidation by a cytochrome P450 monooxygenase, ELONGATED UPPERMOST INTERNODE the product of EUI from bioactive GA4, has shown weak bioactivity on rice seedlings, how 160x,17- epoxyGAs are further deactivated remains elusive. Here, we identify the EUI2 gene, which regulates internode elongation in rice, using a map- based cloning strategy. EUI2 encodes an epoxide hydrolase that hydrolyzes 160x,17- epoxyGAs to 16,17- dihydro- 160x,17- dihydroxyGAs. The eui2 mutants are taller than wild- type plants but are shorter than the eui mutants. However, the levels of known bioactive GAs in the uppermost internodes are not significantly increased in the eui2 mutants. Instead, we show that the eui2 mutants accumulate 160x,17- epoxyGA4 to high levels. We also show that exogenously applied 160x,17- epoxyGA4 is significantly active in elongating the uppermost internode, although not as potent as GA4. Furthermore, we demonstrate that 160x,17- epoxyGA4 can directly interact with the rice GA receptor, GIBBERELLIN INSENSITIVE DWARF1, in vitro. Taken together, the sequential action of EUI and EUI2 results in the stepwise deactivation of GAs during internode elongation in rice. Our data also suggest that the accumulation of a weakly active GA contributes to the mildly tall phenotype of the eui2 mutants.

Keyword :

deactivation deactivation gibberellin gibberellin internode elongation internode elongation plant hormones plant hormones rice rice

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GB/T 7714 Ishida, Toshiaki , Zhang, Yingying , Zhu, Hongbo et al. Stepwise deactivation of gibberellins during rice internode elongation [J]. | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA , 2025 , 122 (23) .
MLA Ishida, Toshiaki et al. "Stepwise deactivation of gibberellins during rice internode elongation" . | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 122 . 23 (2025) .
APA Ishida, Toshiaki , Zhang, Yingying , Zhu, Hongbo , Fudano, Shoko , Peng, Yu , Seto, Yoshiya et al. Stepwise deactivation of gibberellins during rice internode elongation . | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA , 2025 , 122 (23) .
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OsPPG encodes a pseudouridine-5 0-phosphate glycosidase and regulates rice spikelet development SCIE
期刊论文 | 2024 , 12 (3) , 698-708 | CROP JOURNAL
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Abstract :

Florets are the basic structural units of spikelets, and their morphogenesis determines the yield and quality of rice grains. However, whether and how pseudouridine-5 0-phosphate glycosidase participates in rice spikelet development remains an open question. In this study, we identified a novel gene, OsPPG , which encodes a peroxisome-localized pseudouridine-5 0-phosphate glycosidase and regulates the development of rice spikelets. osppg mutants exhibited abnormal sterile lemma, lemma, palea, lodicule, stamens, and pistils; male sterility; shorter panicles; and reduced plant height. OsPPG was found to regulate several OsMADS genes, thereby affecting the morphogenesis of rice spikelets. Furthermore, metabolomics revealed that the OsPPG gene was involved in the decomposition of pseudouridine via the pyrimidine metabolism pathway and may affect the jasmonic acid signaling pathway. These results suggest that OsPPG is a key regulator of rice spikelet development. (c) 2024 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

Keyword :

OsPPG OsPPG Reproductive regulation Reproductive regulation Rice Rice Spikelet development Spikelet development

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GB/T 7714 Chen, Rui , Tian, Dagang , Lin, Yarong et al. OsPPG encodes a pseudouridine-5 0-phosphate glycosidase and regulates rice spikelet development [J]. | CROP JOURNAL , 2024 , 12 (3) : 698-708 .
MLA Chen, Rui et al. "OsPPG encodes a pseudouridine-5 0-phosphate glycosidase and regulates rice spikelet development" . | CROP JOURNAL 12 . 3 (2024) : 698-708 .
APA Chen, Rui , Tian, Dagang , Lin, Yarong , Huang, Jiaqing , Yang, Qinqin , Li, Yupei et al. OsPPG encodes a pseudouridine-5 0-phosphate glycosidase and regulates rice spikelet development . | CROP JOURNAL , 2024 , 12 (3) , 698-708 .
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优质抗病杂交水稻新组合野香优7008的选育
期刊论文 | 2024 , 39 (01) , 85-87 | 杂交水稻
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Abstract :

野香优7008是由福建农林大学作物遗传育种研究所、广西绿海种业有限公司和福建禾丰种业股份有限公司选育的优质抗病籼型三系杂交水稻新组合。该组合抗稻瘟病,米质达部颁1级优质食用稻品种品质标准,于2022年通过福建省主要农作物品种审定委员会的审定。

Keyword :

优质 优质 抗病 抗病 杂交水稻 杂交水稻 选育 选育 野香优7008 野香优7008

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GB/T 7714 马洪丽 , 刘情 , 叶馨 et al. 优质抗病杂交水稻新组合野香优7008的选育 [J]. | 杂交水稻 , 2024 , 39 (01) : 85-87 .
MLA 马洪丽 et al. "优质抗病杂交水稻新组合野香优7008的选育" . | 杂交水稻 39 . 01 (2024) : 85-87 .
APA 马洪丽 , 刘情 , 叶馨 , 姜晓童 , 周雨雪 , 陈舒龙 et al. 优质抗病杂交水稻新组合野香优7008的选育 . | 杂交水稻 , 2024 , 39 (01) , 85-87 .
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Genome-Wide Identification and Characterization of the HAK Gene Family in Quinoa (Chenopodium quinoa Willd.) and Their Expression Profiles under Saline and Alkaline Conditions SCIE
期刊论文 | 2023 , 12 (21) | PLANTS-BASEL
WoS CC Cited Count: 4
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Abstract :

The high-affinity K+ transporter (HAK) family, the most prominent potassium transporter family in plants, which involves K+ transport, plays crucial roles in plant responses to abiotic stresses. However, the HAK gene family remains to be characterized in quinoa (Chenopodium quinoa Willd.). We explored HAKs in quinoa, identifying 30 members (CqHAK1-CqHAK30) in four clusters phylogenetically. Uneven distribution was observed across 18 chromosomes. Furthermore, we investigated the proteins' evolutionary relationships, physicochemical properties, conserved domains and motifs, gene structure, and cis-regulatory elements of the CqHAKs family members. Transcription data analysis showed that CqHAKs have diverse expression patterns among different tissues and in response to abiotic stresses, including drought, heat, low phosphorus, and salt. The expressional changes of CqHAKs in roots were more sensitive in response to abiotic stress than that in shoot apices. Quantitative RT-PCR analysis revealed that under high saline condition, CqHAK1, CqHAK13, CqHAK19, and CqHAK20 were dramatically induced in leaves; under alkaline condition, CqHAK1, CqHAK13, CqHAK19, and CqHAK20 were dramatically induced in leaves, and CqHAK6, CqHAK9, CqHAK13, CqHAK23, and CqHAK29 were significantly induced in roots. Our results establish a foundation for further investigation of the functions of HAKs in quinoa. It is the first study to identify the HAK gene family in quinoa, which provides potential targets for further functional study and contributes to improving the salt and alkali tolerance in quinoa.

Keyword :

alkali stress alkali stress expression profile expression profile HAK gene family HAK gene family quinoa quinoa salt stress salt stress

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GB/T 7714 Chen, Yanqiong , Lin, Yingfeng , Zhang, Shubiao et al. Genome-Wide Identification and Characterization of the HAK Gene Family in Quinoa (Chenopodium quinoa Willd.) and Their Expression Profiles under Saline and Alkaline Conditions [J]. | PLANTS-BASEL , 2023 , 12 (21) .
MLA Chen, Yanqiong et al. "Genome-Wide Identification and Characterization of the HAK Gene Family in Quinoa (Chenopodium quinoa Willd.) and Their Expression Profiles under Saline and Alkaline Conditions" . | PLANTS-BASEL 12 . 21 (2023) .
APA Chen, Yanqiong , Lin, Yingfeng , Zhang, Shubiao , Lin, Zhongyuan , Chen, Songbiao , Wang, Zonghua . Genome-Wide Identification and Characterization of the HAK Gene Family in Quinoa (Chenopodium quinoa Willd.) and Their Expression Profiles under Saline and Alkaline Conditions . | PLANTS-BASEL , 2023 , 12 (21) .
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水稻耐盐分子机制与分子改良
期刊论文 | 2022 , 23 (03) , 644-653 | 植物遗传资源学报
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盐碱地是农业种植的重要后备耕地资源,但其高盐碱度特性严重制约了作物的种植。培育适宜盐碱地种植的作物,对保障粮食安全具有重要意义。近年来,耐盐水稻成为盐碱地改良与利用的首选粮食作物,因此,耐盐性遗传改良已成为水稻遗传育种的一个重要研究方向。高浓度盐胁迫对水稻各生育期的生长发育都会造成影响,导致水稻产量与品质显著下降。水稻耐盐分子机制的研究对培育耐盐水稻品种具有重要指导意义。本文从离子稳态调节、渗透调节、抗氧化调节、气孔调节以及信号分子调节等5个方面综述了水稻耐盐适应性分子机制研究的最新进展,进一步介绍了国内外水稻耐盐性遗传改良的情况,并重点总结了负调控水稻耐盐性功能基因的鉴定,以及基于基因组编辑技术的水稻耐盐性分子改良;同时还探讨了未来值得重点关注的方向及策略,以期为高效培育耐盐性强、综合性状优良的水稻品种提供参考。

Keyword :

分子改良 分子改良 基因组编辑 基因组编辑 水稻 水稻 盐胁迫 盐胁迫 耐盐机制 耐盐机制 负调控基因 负调控基因

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GB/T 7714 曲梦宇 , 许惠滨 , 陈静 et al. 水稻耐盐分子机制与分子改良 [J]. | 植物遗传资源学报 , 2022 , 23 (03) : 644-653 .
MLA 曲梦宇 et al. "水稻耐盐分子机制与分子改良" . | 植物遗传资源学报 23 . 03 (2022) : 644-653 .
APA 曲梦宇 , 许惠滨 , 陈静 , 梁廷敏 , 韩艺娟 , 陈燕琼 et al. 水稻耐盐分子机制与分子改良 . | 植物遗传资源学报 , 2022 , 23 (03) , 644-653 .
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Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice SCIE
期刊论文 | 2021 , 21 (1) | BMC PLANT BIOLOGY
WoS CC Cited Count: 11
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BackgroundMethyl-CpG-binding domain (MBD) proteins play important roles in epigenetic gene regulation, and have diverse molecular, cellular, and biological functions in plants. MBD proteins have been functionally characterized in various plant species, including Arabidopsis, wheat, maize, and tomato. In rice, 17 sequences were bioinformatically predicted as putative MBD proteins. However, very little is known regarding the function of MBD proteins in rice.ResultsWe explored the expression patterns of the rice OsMBD family genes and identified 13 OsMBDs with active expression in various rice tissues. We further characterized the function of a rice class I MBD protein OsMBD707, and demonstrated that OsMBD707 is constitutively expressed and localized in the nucleus. Transgenic rice overexpressing OsMBD707 displayed larger tiller angles and reduced photoperiod sensitivity-delayed flowering under short day (SD) and early flowering under long day (LD). RNA-seq analysis revealed that overexpression of OsMBD707 led to reduced photoperiod sensitivity in rice and to expression changes in flowering regulator genes in the Ehd1-Hd3a/RFT1 pathway.ConclusionThe results of this study suggested that OsMBD707 plays important roles in rice growth and development, and should lead to further studies on the functions of OsMBD proteins in growth, development, or other molecular, cellular, and biological processes in rice.

Keyword :

Flowering time Flowering time MBD MBD Overexpression Overexpression Photoperiod sensitivity Photoperiod sensitivity Rice Rice Tiller angle Tiller angle

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GB/T 7714 Qu, Mengyu , Zhang, Zhujian , Liang, Tingmin et al. Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice [J]. | BMC PLANT BIOLOGY , 2021 , 21 (1) .
MLA Qu, Mengyu et al. "Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice" . | BMC PLANT BIOLOGY 21 . 1 (2021) .
APA Qu, Mengyu , Zhang, Zhujian , Liang, Tingmin , Niu, Peipei , Wu, Mingji , Chi, Wenchao et al. Overexpression of a methyl-CpG-binding protein gene OsMBD707 leads to larger tiller angles and reduced photoperiod sensitivity in rice . | BMC PLANT BIOLOGY , 2021 , 21 (1) .
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Bulked Segregant Analysis Coupled with Whole-Genome Sequencing (BSA-Seq) Mapping Identifies a Novel pi21 Haplotype Conferring Basal Resistance to Rice Blast Disease SCIE
期刊论文 | 2020 , 21 (6) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
WoS CC Cited Count: 26
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Basal or partial resistance has been considered race-non-specific and broad-spectrum. Therefore, the identification of genes or quantitative trait loci (QTLs) conferring basal resistance and germplasm containing them is of significance in breeding crops with durable resistance. In this study, we performed a bulked segregant analysis coupled with whole-genome sequencing (BSA-seq) to identify QTLs controlling basal resistance to blast disease in an F-2 population derived from two rice varieties, 02428 and LiXinGeng (LXG), which differ significantly in basal resistance to rice blast. Four candidate QTLs, qBBR-4, qBBR-7, qBBR-8, and qBBR-11, were mapped on chromosomes 4, 7, 8, and 11, respectively. Allelic and genotypic association analyses identified a novel haplotype of the durable blast resistance gene pi21 carrying double deletions of 30 bp and 33 bp in 02428 (pi21-2428) as a candidate gene of qBBR-4. We further assessed haplotypes of Pi21 in 325 rice accessions, and identified 11 haplotypes among the accessions, of which eight were novel types. While the resistant pi21 gene was found only in japonica before, three Chinese indica varieties, ShuHui881, Yong4, and ZhengDa4Hao, were detected carrying the resistant pi21-2428 allele. The pi21-2428 allele and pi21-2428-containing rice germplasm, thus, provide valuable resources for breeding rice varieties, especially indica rice varieties, with durable resistance to blast disease. Our results also lay the foundation for further identification and functional characterization of the other three QTLs to better understand the molecular mechanisms underlying rice basal resistance to blast disease.

Keyword :

blast disease blast disease haplotype haplotype partial resistance partial resistance pi21 pi21 rice rice

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GB/T 7714 Liang, Tingmin , Chi, Wenchao , Huang, Likun et al. Bulked Segregant Analysis Coupled with Whole-Genome Sequencing (BSA-Seq) Mapping Identifies a Novel pi21 Haplotype Conferring Basal Resistance to Rice Blast Disease [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2020 , 21 (6) .
MLA Liang, Tingmin et al. "Bulked Segregant Analysis Coupled with Whole-Genome Sequencing (BSA-Seq) Mapping Identifies a Novel pi21 Haplotype Conferring Basal Resistance to Rice Blast Disease" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 21 . 6 (2020) .
APA Liang, Tingmin , Chi, Wenchao , Huang, Likun , Qu, Mengyu , Zhang, Shubiao , Chen, Zi-Qiang et al. Bulked Segregant Analysis Coupled with Whole-Genome Sequencing (BSA-Seq) Mapping Identifies a Novel pi21 Haplotype Conferring Basal Resistance to Rice Blast Disease . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2020 , 21 (6) .
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The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication SCIE
期刊论文 | 2019 , 51 (5) , 865-, | NATURE GENETICS
WoS CC Cited Count: 379
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High oil and protein content make tetraploid peanut a leading oil and food legume. Here we report a high-quality peanut genome sequence, comprising 2.54 Gb with 20 pseudomolecules and 83,709 protein-coding gene models. We characterize gene functional groups implicated in seed size evolution, seed oil content, disease resistance and symbiotic nitrogen fixation. The peanut B subgenome has more genes and general expression dominance, temporally associated with long-terminal-repeat expansion in the A subgenome that also raises questions about the A-genome progenitor. The polyploid genome provided insights into the evolution of Arachis hypogaea and other legume chromosomes. Resequencing of 52 accessions suggests that independent domestications formed peanut ecotypes. Whereas 0.42-0.47 million years ago (Ma) polyploidy constrained genetic variation, the peanut genome sequence aids mapping and candidate-gene discovery for traits such as seed size and color, foliar disease resistance and others, also providing a cornerstone for functional genomics and peanut improvement.

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GB/T 7714 Zhuang, Weijian , Chen, Hua , Yang, Meng et al. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication [J]. | NATURE GENETICS , 2019 , 51 (5) : 865-, .
MLA Zhuang, Weijian et al. "The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication" . | NATURE GENETICS 51 . 5 (2019) : 865-, .
APA Zhuang, Weijian , Chen, Hua , Yang, Meng , Wang, Jianping , Pandey, Manish K. , Zhang, Chong et al. The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication . | NATURE GENETICS , 2019 , 51 (5) , 865-, .
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水稻稀穗突变体的遗传分析及其基因的初步定位 CSCD PKU
期刊论文 | 2016 , 14 (08) , 2106-2112 | 分子植物育种
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水稻的穗形与其产量关系密切,也是研究的一大热点。利用60Co-γ射线辐射诱变水稻68902B,筛选到一个水稻稀穗突变体,暂命名为Oslp(Oryza sativa lax panicle)。研究了该突变体的主要农艺性状与稀穗的遗传方式,并对稀穗突变基因Oslp进行了分子定位。结果显示稀穗突变体Oslp的每穗粒数为104粒、二次枝梗数目为7个,它们都显著地少于原品种68902B每穗粒数的124粒和二次枝梗数目的 20个。遗传分析揭示突变体Oslp的稀穗性状受一对隐性核基因控制。将(籼稻品种261S×稀穗突变体Oslp)F2代中的稀穗个体作为定位群体,结合BSA和SSR分子标记技术,将基因Oslp定位在第7号染色体短臂的2个分子标记FR-3和FR-4之间,基因Oslp与FR-3和FR-4的遗传距离分别为0.6 c M和0.8 c M。

Keyword :

基因定位 基因定位 水稻 水稻 稀穗突变体Oslp 稀穗突变体Oslp 遗传分析 遗传分析

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GB/T 7714 马洪丽 , 温婵 , 吕巧香 et al. 水稻稀穗突变体的遗传分析及其基因的初步定位 [J]. | 分子植物育种 , 2016 , 14 (08) : 2106-2112 .
MLA 马洪丽 et al. "水稻稀穗突变体的遗传分析及其基因的初步定位" . | 分子植物育种 14 . 08 (2016) : 2106-2112 .
APA 马洪丽 , 温婵 , 吕巧香 , 张书标 . 水稻稀穗突变体的遗传分析及其基因的初步定位 . | 分子植物育种 , 2016 , 14 (08) , 2106-2112 .
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eui基因对水稻光温敏核不育系主要农艺性状的影响 CSCD
期刊论文 | 2014 , 35 (12) , 2328-2331 | 热带作物学报
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eui基因具有遗传解除水稻不育系包穗的功能,被誉为杂交稻种子生产的第四遗传因子,现已成功地应用于杂交稻的生产。研究不同eui基因对水稻光温敏核不育系主要农艺性状的影响。结果表明,eui基因具有遗传解除水稻光温敏核不育系包穗的功效,可育期解除不育系包穗的遗传效应显著地好于不育期,且eui1基因遗传解除包穗的效应强于eui2基因。eui基因的效应并未影响到光温敏核不育系的育性和花粉不育的类型。

Keyword :

eui基因 eui基因 光温敏核不育系 光温敏核不育系 农艺性状 农艺性状 水稻 水稻

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GB/T 7714 马洪丽 , 谢其男 , 张书标 . eui基因对水稻光温敏核不育系主要农艺性状的影响 [J]. | 热带作物学报 , 2014 , 35 (12) : 2328-2331 .
MLA 马洪丽 et al. "eui基因对水稻光温敏核不育系主要农艺性状的影响" . | 热带作物学报 35 . 12 (2014) : 2328-2331 .
APA 马洪丽 , 谢其男 , 张书标 . eui基因对水稻光温敏核不育系主要农艺性状的影响 . | 热带作物学报 , 2014 , 35 (12) , 2328-2331 .
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