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学者姓名：黄锦文
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Abstract ：
Rice straw return is a crucial practice for recycling organic matter in agricultural systems, but long-term incorporation can lead to recalcitrant carbon (C) accumulation, suppress nutrient release, and inhibit microbial metabolism, thereby reducing soil nutrient availability and crop productivity. Lime and decomposing agents are common supplementary measures, yet their rhizosphere microbiome-mediated mechanisms remain poorly understood. Here, we conducted a field study on a long-term tobacco-rice rotation straw return platform in Southern China (established 2003) during 2020-2021, including straw removal (RM), conventional straw return (SR), and ecological enhancement treatments (SR + L: lime; SR + A: decomposing agent). We evaluated crop productivity, soil nutrient availability, and microbial nutrient acquisition, and profiled rhizosphere microbial responses during the tobacco season using high-throughput sequencing. RM and SR showed no significant difference in yields, whereas SR suppressed soil C and nitrogen (N) availability and reduced tobacco dry matter and N accumulation. In contrast, SR + L and SR + A enhanced rhizosphere nutrient acquisition: SR + L increased Nacquiring enzyme activities by 29.6-35.7 %, and SR + A increased C-acquiring activities by 35.3-49.4 %. Both treatments improved soil carbon and nitrogen availability in the tobacco season by 5.6-8.4 % and 1.5-1.7 %, and in the rice season by 4.5-5.8 % and 1.6-1.9 %, while also enhancing phosphorus (P) availability, alleviating microbial carbon and nitrogen limitation, and increasing tobacco and rice yields by 16.5 % and 4.2 %, respectively. Under SR, key rhizosphere microbial communities exhibited inhibited tricarboxylic acid cycle, glycolysis, and N and P transformation pathways, explaining reduced nutrient availability and crop productivity. Ecological enhancement activated metabolic pathways, optimized fungal communities, promoted saprotrophic fungi, and suppressed pathogens, achieving multi-pathway, multi-element functional integration that improved soil ecological function and system stability, while identifying the core species Acidothermus, which was significantly positively correlated with nutrient availability. These findings reveal the coupled mechanisms of nutrient limitation and microbial functional impairment under long-term straw return and highlight the pivotal roles of keystone microbial communities and core species in ecological restoration and yield improvement, providing a theoretical basis and practical guidance for optimized straw return management and precise ecological interventions.
Keyword ：
Crop rotation pattern Crop rotation pattern Microbial function Microbial function Nutrient availability Nutrient availability Straw return Straw return
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| GB/T 7714 | Zhang, Bianhong , Li, Rikun , Chen, Zhicheng et al. Optimizing soil microbial functions under long-term straw return enhances nutrient acquisition, thereby improving nutrient availability and crop productivity in tobacco-rice rotation systems of southern China [J]. | AGRICULTURE ECOSYSTEMS & ENVIRONMENT , 2026 , 397 . |
| MLA | Zhang, Bianhong et al. "Optimizing soil microbial functions under long-term straw return enhances nutrient acquisition, thereby improving nutrient availability and crop productivity in tobacco-rice rotation systems of southern China" . | AGRICULTURE ECOSYSTEMS & ENVIRONMENT 397 (2026) . |
| APA | Zhang, Bianhong , Li, Rikun , Chen, Zhicheng , Zeng, Chunli , Liao, Songquan , Liu, Yazhou et al. Optimizing soil microbial functions under long-term straw return enhances nutrient acquisition, thereby improving nutrient availability and crop productivity in tobacco-rice rotation systems of southern China . | AGRICULTURE ECOSYSTEMS & ENVIRONMENT , 2026 , 397 . |
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Key message203 bZIP members were identified in each G. barbadence and G. hirsutum and GhbZIP017 from the A subfamily was a novel drought-related member in cotton.AbstractThe basic leucine zipper (bZIP) transcription factors are widely distributed in plants, but the evolutionary history is still unclear in allotetraploid Gossypium barbadense and Gossypium hirsutum. In this study, 203 bZIP members were identified in each G. barbadense and G. hirsutum. The bZIP members could be further divided into 13 subfamilies. A total of 227 gene duplication events were discovered in two allotetraploid cotton species and mainly occurred in the cotton ancestor. Furthermore, the bZIP family had a conserved evolutionary history in two allotetraploid cotton species. Meanwhile, the bZIP members had tissue-specific expression levels. Moreover, through the RNA-seq analysis, GhbZIP017 from the A subfamily played an important role in drought stress response. GhbZIP017 was localized in the nucleus and acted as a transcriptional repressor. The expression levels of GhbZIP017 in leaves and roots could be highly induced by drought stress. GhbZIP017 overexpression in Arabidopsis could enhance drought tolerance with a higher survival rate, lower membrane ion leakage, higher SOD and POD activity, lower MDA content, and higher expression levels of some drought-related genes. Overall, these results could help us uncover the evolutionary history of the bZIP members in cotton and provide a candidate gene GhbZIP017 for drought breeding in G. hirsutum.
Keyword ：
Cotton Cotton Drought stress response Drought stress response Evolutionary history Evolutionary history GhbZIP017 GhbZIP017 The bZIP family The bZIP family
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| GB/T 7714 | Ye, Fangting , He, Jing , Li, Zhaowei et al. Molecular evolution of the bZIP family in allotetraploid Gossypium and GhbZIP017 function under drought stress [J]. | PLANT CELL REPORTS , 2025 , 44 (10) . |
| MLA | Ye, Fangting et al. "Molecular evolution of the bZIP family in allotetraploid Gossypium and GhbZIP017 function under drought stress" . | PLANT CELL REPORTS 44 . 10 (2025) . |
| APA | Ye, Fangting , He, Jing , Li, Zhaowei , Wu, Huanyu , Zhang, Ziyou , Fu, Dan et al. Molecular evolution of the bZIP family in allotetraploid Gossypium and GhbZIP017 function under drought stress . | PLANT CELL REPORTS , 2025 , 44 (10) . |
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Introduction Lime and biochar are widely utilized to enhance nitrogen utilization in crops grown on acidic soils, though each has its own set of limitations. Understanding their combined effects is crucial for optimizing soil remediation strategies.Methods This study investigates the impact of lime and biochar on nitrogen utilization efficiency (NUE) in a tobacco monoculture system, which has been practiced for 20 years on acidified soils in Fuzhou, southeastern China, over the period from 2021 to 2022. Four treatments were applied: control (CK), lime alone (L), biochar alone (B), and a lime-biochar combination (L+B).Results The results indicated that all treatments significantly improved NUE, with increases ranging from 20.07% to 27.17% compared to CK. Biochar (B) was more effective than lime (L), and the combined treatment (L+B) showed comparable effects to biochar alone. Correlation analysis revealed that increases in soil pH and exchangeable base cations facilitated nitrogen transformation, thereby enhancing NUE. Lime treatments (L, L+B) promoted nitrification potential in rhizosphere soil, whereas biochar application (B, L+B) resulted in elevated nitrate nitrogen content. Microbial functional analysis indicated that lime (L, L+B) enhanced nitrification, while biochar (B, L+B) fostered dissimilatory nitrate reduction, thereby improving nitrogen retention. Pearson correlation analysis demonstrated a strong positive relationship between dissimilatory nitrate reduction and both soil alkali-hydrolyzable nitrogen and nitrate nitrogen contents.Conclusion These findings suggest that lime enhances nitrification, while biochar promotes nitrate retention, together increasing soil nitrogen availability. The combined application of lime and biochar integrates these benefits, yielding results comparable to biochar alone. This study offers valuable insights into the synergistic use of lime and biochar for mitigating soil acidification and optimizing nitrogen management in agricultural systems.
Keyword ：
acidic soil acidic soil biochar biochar lime lime nitrogen utilization efficiency nitrogen utilization efficiency tobacco tobacco
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| GB/T 7714 | Zhang, Bianhong , Tang, Lina , Chen, Zhicheng et al. Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping [J]. | FRONTIERS IN PLANT SCIENCE , 2025 , 16 . |
| MLA | Zhang, Bianhong et al. "Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping" . | FRONTIERS IN PLANT SCIENCE 16 (2025) . |
| APA | Zhang, Bianhong , Tang, Lina , Chen, Zhicheng , Chen, Xiaoyan , You, Lindong , Pan, Ruixin et al. Comparative and synergistic impacts of lime and biochar on soil properties, nitrogen transformation, and microbial function in acidic soils under tobacco cropping . | FRONTIERS IN PLANT SCIENCE , 2025 , 16 . |
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为探究生物炭用量对烟草青枯病发生及土壤细菌群落结构和功能的影响，以植烟病圃土壤为研究对象，设置5个炭/土比0、1.5%、3.0%、4.5%、6.0%（记为TS0、TS15、TS30、TS45、TS60）的盆栽试验，研究烤烟青枯病发病情况、土壤农化性状、细菌群落结构与功能。结果表明，生物炭可有效减轻烟草青枯病发病情况，以炭/土比3.0%效果最佳。生物炭处理均可显著增加土壤pH值及有机质含量、改善土壤养分比例。冗余分析（RDA）结果表明，土壤pH值及养分是影响烟草青枯病的重要因子，提升土壤pH值及养分含量可有效减轻青枯病发生程度。进一步分析发现，生物炭可提高土壤细菌Alpha多样性，与对照相比，炭/土比1.5%～6.0%处理Chaol指数提高了8.24%～26.50%，变形菌门丰度分别增加了2.28、9.70、5.40、5.28个百分点，而酸杆菌门丰度分别降低了5.48、7.05、8.09、7.06个百分点。表型预测结果表明，生物炭可通过提高变形菌门丰度及降低酸杆菌门丰度来减少潜在致病菌丰度；与对照相比，炭/土比3.0%处理的芽孢杆菌属、鞘氨醇单胞菌属、出芽单胞菌属丰度分别增加5.77、0.24、0.36个百分点，罗河杆菌属丰度降低1.70个百分点，且雷尔氏菌属丰度随着施炭量的增加而逐渐减小。此外，生物炭可提高土壤氨基酸代谢、细胞生长与死亡功能类群丰度，且土壤pH值、有机质、速效钾、全素养分含量与氨基酸代谢、细胞生长与死亡功能具有正向效应，而罗河杆菌属与其具有负向效应。综上，生物炭可显著改善土壤养分比例，进而改善土壤细菌群落结构和功能特性、激发细菌群落氨基酸代谢等功能，提升烟株抗病性，减少青枯病的发生程度，以炭/土比3.0%～4.5%防控烟株青枯病效果较好。本研究结果为烟草种植中的病害防控和土壤改良提供了技术参考。
Keyword ：
功能预测 功能预测 土壤农化性状 土壤农化性状 土壤细菌群落结构 土壤细菌群落结构 烟草青枯病 烟草青枯病 生物炭 生物炭
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| GB/T 7714 | 潘美清 , 唐莉娜 , 吴长征 et al. 生物炭用量对烟草青枯病及土壤细菌群落的影响 [J]. | 核农学报 , 2024 , 38 (06) : 1186-1195 . |
| MLA | 潘美清 et al. "生物炭用量对烟草青枯病及土壤细菌群落的影响" . | 核农学报 38 . 06 (2024) : 1186-1195 . |
| APA | 潘美清 , 唐莉娜 , 吴长征 , 杨铭榆 , 张汴泓 , 黄锦文 et al. 生物炭用量对烟草青枯病及土壤细菌群落的影响 . | 核农学报 , 2024 , 38 (06) , 1186-1195 . |
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Soil acidification represents a severe threat to tobacco cultivation regions in South China, exacerbating bacterial wilt caused by Ralstonia solanacearum. The comprehension of the underlying mechanisms that facilitate the restoration of rhizosphere microbial communities in "healthy soils" is imperative for ecologically managing tobacco bacterial wilt. This study focuses on acidified tobacco soils that have been subjected to continuous cultivation for 20 years. The experimental treatments included lime (L), biochar (B), and a combination of lime and biochar (L+B), in addition to a control group (CK). Utilizing rhizosphere biology and niche theory, we assessed disease suppression effects, changes in soil properties, and the co-evolution of the rhizosphere bacterial community. Each treatment significantly reduced tobacco bacterial wilt by 16.67% to 20.14% compared to the control group (CK) (p < 0.05) and increased yield by 7.86% to 27.46% (p < 0.05). The biochar treatment (B) proved to be the most effective, followed by the lime-biochar combination (L+B). The key factors controlling wilt were identified through random forest regression analysis as an increase in soil pH and exchangeable bases, along with a decrease in exchangeable acidity. However, lime treatment alone led to an increase in soil bulk density and a decrease in available nutrients, whereas both biochar and lime-biochar treatments significantly improved these parameters (p < 0.05). No significant correlation was found between the abundance of Ralstonia and wilt incidence. Nonetheless, all treatments significantly expanded the ecological niche breadth and average variation degree (AVD), enhanced positive interactions and cohesion within the community, and intensified negative interactions involving Ralstonia. This study suggests that optimizing community niches and enhancing pathogen antagonism are key mechanisms for mitigating tobacco wilt in acidified soils. It recommends using lime-biochar mixtures as soil amendments due to their potential ecological and economic benefits. This study offers valuable insights for disease control strategies and presents a novel perspective for research on Solanaceous crops.
Keyword ：
acidification amelioration acidification amelioration bacterial wilt bacterial wilt rhizosphere bacteria rhizosphere bacteria synergistic co-evolution synergistic co-evolution tobacco tobacco
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| GB/T 7714 | Zhang, Bian-hong , Tang, Li-na , Li, Ri-kun et al. Synergistic co-evolution of rhizosphere bacteria in response to acidification amelioration strategies: impacts on the alleviation of tobacco wilt and underlying mechanisms [J]. | FRONTIERS IN MICROBIOLOGY , 2024 , 15 . |
| MLA | Zhang, Bian-hong et al. "Synergistic co-evolution of rhizosphere bacteria in response to acidification amelioration strategies: impacts on the alleviation of tobacco wilt and underlying mechanisms" . | FRONTIERS IN MICROBIOLOGY 15 (2024) . |
| APA | Zhang, Bian-hong , Tang, Li-na , Li, Ri-kun , Pan, Rui-xin , You, Lin-dong , Chen, Xiao-yan et al. Synergistic co-evolution of rhizosphere bacteria in response to acidification amelioration strategies: impacts on the alleviation of tobacco wilt and underlying mechanisms . | FRONTIERS IN MICROBIOLOGY , 2024 , 15 . |
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Agronomic measures are the key to promote the sustainable development of ratoon rice by reducing the damage from mechanical crushing to the residual stubble of the main crop, thereby mitigating the impact on axillary bud sprouting and yield formation in ratoon rice. This study used widely recommended conventional rice Jiafuzhan and hybrid rice Yongyou 2640 as the test materials to conduct a four -factor block design field experiment in a greenhouse of the experimental farm of Fujian Agricultural and Forestry University, China from 2018 to 2019. The treatments included fertilization and no fertilization, alternate wetting and drying irrigation and continuous water flooding irrigation, and plots with and without artificial crushing damage on the rice stubble. At the same time, a 13 C stable isotope in -situ detection technology was used to fertilize the pot experiment. The results showed significant interactions among varieties, water management, nitrogen application and stubble status. Relative to the long-term water flooding treatment, the treatment with sequential application of nitrogen fertilizer coupled with moderate field drought for root -vigor and tiller promotion before and after harvesting of the main crop, significantly improved the effective tillers from low position nodes. This in turn increased the effective panicles per plant and grains per panicle by reducing the influence of artificial crushing damage on rice stubble and achieving a high yield of the regenerated rice. Furthermore, the partitioning of 13 C assimilates to the residual stubble and its axillary buds were significantly improved at the mature stage of the main crop, while the translocation rate to roots and rhizosphere soil was reduced at the later growth stage of ratooning season rice. This was triggered by the metabolism of hormones and polyamines at the stem base regulated by the interaction of water and fertilizer at this time. We therefore suggest that to achieve a high yield of ratoon rice with low stubble height under mechanized harvesting, the timely application of nitrogen fertilizer is fundamental, coupled with moderate field drying for root -vigor preservation and tiller promotion before and after the mechanical harvesting of the main crop.
Keyword ：
mechanized harvesting mechanized harvesting ratoon rice ratoon rice rice stubble rice stubble yield attributes yield attributes
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| GB/T 7714 | Zou, Jingnan , Pang, Ziqin , Li, Zhou et al. The underlying mechanism of variety-water-nitrogen-stubble damage interactions on yield formation in ratoon rice with low stubble height under mechanized harvesting [J]. | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (3) : 806-823 . |
| MLA | Zou, Jingnan et al. "The underlying mechanism of variety-water-nitrogen-stubble damage interactions on yield formation in ratoon rice with low stubble height under mechanized harvesting" . | JOURNAL OF INTEGRATIVE AGRICULTURE 23 . 3 (2024) : 806-823 . |
| APA | Zou, Jingnan , Pang, Ziqin , Li, Zhou , Guo, Chunlin , Lin, Hongmei , Li, Zheng et al. The underlying mechanism of variety-water-nitrogen-stubble damage interactions on yield formation in ratoon rice with low stubble height under mechanized harvesting . | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (3) , 806-823 . |
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Ratoon rice utilizes the axillary buds sprouting from the remaining stubble of the main crop after harvest to form panicles, enabling a second harvest. However, mechanized harvesting often resulted in damage to the rice stubbles in the rolling zone, potentially leading to reduced yield. Enhancing the number of tillers in the rolling zone through optimized agronomic measures was crucial for achieving higher yield. However, research on water and fertilizer management corresponding to low stubble ratoon rice under mechanized harvesting was limited. A two-factor experiment was conducted to assess the impacts of water regimes (flooded, FL; alternate wetting and drying, AWD) and nitrogen fertilizer management (0 kg N ha-1, CK; Tiller promotion fertilizer 90 kg N ha-1, N; Delayed nitrogen application: Tiller promotion fertilizer 60 kg N ha-1 + Booting stage fertilizer 30 kg N ha-1, SN) on the yield formation, greenhouse gas emissions, and carbon footprint of low stubble ratoon rice. The results indicated significant effects of water regimes and nitrogen fertilizer on yield. Compared to N-FL, SN-AWD significantly increased average annual yield by 25.39%. The increment in tillers in the rolling zone ranged from 36.58% to 72.72% under AWD compared to flooding, and SN-AWD significantly increased the ratoon ability of the basal first and second nodes compared to N-FL. Soil quality index and ecosystem multifunctionality under SN-AWD increased by 32.37% and 9.05 times, respectively, compared to N-FL, resulting in significant increases in root length, root surface area, and root volume, consequently enhancing pre-anthesis and post-anthesis dry matter accumulation and ultimately improving yield. Although N2O emissions increased under SN-AWD compared to N-FL, CH4 cumulative emissions decreased significantly by 37.86% on average over two years, leading to a 23.02% reduction in total greenhouse gas emissions and a 38.62% reduction in carbon footprint. SN-AWD attained maximum net ecosystem economic benefit (NEEB), increasing by 35.42% compared to N-FL. Overall, the comprehensive analysis suggested that SN-AWD was a sustainable water and fertilizer management approach beneficial for balancing ratoon season yields, environmental footprint, and economic benefits. © 2024, The Authors. All rights reserved.
Keyword ：
Carbon footprint Carbon footprint Ecosystems Ecosystems Emission control Emission control Floods Floods Gas emissions Gas emissions Greenhouse gases Greenhouse gases Harvesting Harvesting Nitrogen fertilizers Nitrogen fertilizers Sustainable development Sustainable development Water conservation Water conservation
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| GB/T 7714 | Lan, Chaojie , Zou, Jingnan , Xu, Hailong et al. Enhanced Strategies for Water and Fertilizer Management to Optimize Yields and Promote Environmental Sustainability in the Mechanized Harvesting of Ratoon Rice in Southeast China [J]. | SSRN , 2024 . |
| MLA | Lan, Chaojie et al. "Enhanced Strategies for Water and Fertilizer Management to Optimize Yields and Promote Environmental Sustainability in the Mechanized Harvesting of Ratoon Rice in Southeast China" . | SSRN (2024) . |
| APA | Lan, Chaojie , Zou, Jingnan , Xu, Hailong , Qin, Bin , Li, Jinying , Chen, Ting et al. Enhanced Strategies for Water and Fertilizer Management to Optimize Yields and Promote Environmental Sustainability in the Mechanized Harvesting of Ratoon Rice in Southeast China . | SSRN , 2024 . |
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Ratoon rice utilizes the axillary buds sprouting from the remaining stubble of the main crop after harvest to form panicles, enabling a second harvest. However, mechanized harvesting often resulted in damage to the rice stubbles in the rolling zone, potentially leading to reduced yield. Enhancing the number of tillers in the rolling zone through optimized agronomic measures was crucial for achieving higher yield. However, research on water and fertilizer management corresponding to low stubble ratoon rice under mechanized harvesting was limited. A two-factor experiment was conducted to assess the impacts of water regimes (flooded; alternate wetting and drying) and nitrogen fertilizer management (0 kg N ha(-1); Tiller promotion fertilizer 90 kg N ha(-1); Split nitrogen application: Tiller promotion fertilizer 60 kg N ha(-1) + Booting stage fertilizer 30 kg N ha(-1)) on the yield formation, greenhouse gas emissions, and carbon footprint of low stubble ratoon rice. The results indicated significant effects of water regimes and nitrogen fertilizer on yield. Compared to single application of tillerpromoting fertilizer coupled with continuous flooding (N-FL), Split application of tiller-promoting fertilizer coupled with alternate wetting and drying (SN-AWD) significantly increased average annual yield by 25.4%. SNAWD significantly increased the ratoon ability of the basal first and second nodes compared to N-FL. The soil quality index and ecosystem multifunctionality under SN-AWD increased by an average of 32.37% and 10.16 times, respectively, compared to N-FL. This increase resulted in significant enhancements in root length and root surface area, consequently improving pre-anthesis and post-anthesis dry matter accumulation and ultimately enhancing yield. Although N2O emissions increased under SN-AWD compared to N-FL, CH4 cumulative emissions decreased significantly by 37.86% on average over two years, leading to a 23.02% reduction in total greenhouse gas emissions and a 38.62% reduction in carbon footprint per unit grain. SN-AWD attained maximum net ecosystem economic benefit (NEEB), increasing by 35.42% compared to N-FL. Overall, the comprehensive analysis suggested that SN-AWD was a sustainable water and fertilizer management approach beneficial for balancing ratoon season yields, environmental footprint, and economic benefits.
Keyword ：
Axillary bud Axillary bud Carbon footprint Carbon footprint Greenhouse gas emissions Greenhouse gas emissions Ratoon rice Ratoon rice Yield Yield
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| GB/T 7714 | Lan, Chaojie , Zou, Jingnan , Xu, Hailong et al. Enhanced strategies for water and fertilizer management to optimize yields and promote environmental sustainability in the mechanized harvesting of ratoon rice in Southeast China [J]. | AGRICULTURAL WATER MANAGEMENT , 2024 , 302 . |
| MLA | Lan, Chaojie et al. "Enhanced strategies for water and fertilizer management to optimize yields and promote environmental sustainability in the mechanized harvesting of ratoon rice in Southeast China" . | AGRICULTURAL WATER MANAGEMENT 302 (2024) . |
| APA | Lan, Chaojie , Zou, Jingnan , Xu, Hailong , Qin, Bin , Li, Jinying , Chen, Ting et al. Enhanced strategies for water and fertilizer management to optimize yields and promote environmental sustainability in the mechanized harvesting of ratoon rice in Southeast China . | AGRICULTURAL WATER MANAGEMENT , 2024 , 302 . |
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【目的】在烟-稻复种连作系统中，探讨烟后作水稻茎鞘物质转运及其产量对减氮配施生物炭处理的响应，以期为复种连作土壤改良提供科学依据。【方法】以杂交水稻甬优1540为材料，常规施氮栽培为对照(T0)，在烤烟收获后全土层施用30 t/hm2生物炭的前提下，设置纯氮不减施(T1)、纯氮减施10%(T2)、纯氮减施20%(T3)和纯氮减施30%(T4)处理，分析了水稻产量变化与茎鞘同化物积累、转运的关系及其调控机制。【结果】结果表明，2年田间试验各处理产量均表现为T1>T2>T3>T0>T4，其中减氮10%～20%处理(T2和T3)与未减氮处理(T1)之间差异不显著，但均显著高于T0和T4,T2与T3处理2年平均实际产量分别比对照提高了13.94%和13.46%。收获指数方面，T2和T3处理均显著高于其他处理，各处理谷草比表现为T3>T2>T4>T1>T0。生物炭处理并适当减氮下抽穗期叶片净光合速率及SPAD值均显著高于对照。T1处理分蘖数峰值最高，但其分蘖成穗率却显著低于T2和T3处理。在茎鞘物质输出率和茎鞘物质转化率上，T3与T2处理差异不显著，但均显著高于其他处理；茎鞘非结构性碳水化合物(Non-Structural Carbohydrate,NSC)转运量、转运率及籽粒贡献率与茎鞘物质输出率和茎鞘物质转化率表现一致。茎鞘蔗糖相关酶活性分析表明，T3和T2处理水稻灌浆早中期α-淀粉酶、β-淀粉酶、蔗糖合成酶(SS)、蔗糖磷酸合成酶(SPS)活性均最高，但抽穗后20 d这些酶活性则以T1处理最高。【结论】生物炭可改善水稻灌浆期叶片光合性能，减施10%～20%氮肥有利于提高水稻抽穗期茎鞘蔗糖合成与转运酶活性，促进茎鞘物质的积累与转运，提高水稻收获指数，实现“减氮保产”的栽培目标。
Keyword ：
NSC NSC 减氮 减氮 水稻 水稻 烟-稻轮作 烟-稻轮作 生物炭 生物炭
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| GB/T 7714 | 杨铭榆 , 陈志诚 , 潘美清 et al. 烟-稻轮作下减氮配施生物炭对水稻茎鞘同化物转运和产量形成的影响 [J]. | 中国水稻科学 , 2024 , 38 (05) : 555-566 . |
| MLA | 杨铭榆 et al. "烟-稻轮作下减氮配施生物炭对水稻茎鞘同化物转运和产量形成的影响" . | 中国水稻科学 38 . 05 (2024) : 555-566 . |
| APA | 杨铭榆 , 陈志诚 , 潘美清 , 张汴泓 , 潘睿欣 , 尤林东 et al. 烟-稻轮作下减氮配施生物炭对水稻茎鞘同化物转运和产量形成的影响 . | 中国水稻科学 , 2024 , 38 (05) , 555-566 . |
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【目的】探讨深耕栽培对烤烟产量产值及根际土壤特性和微生态系统的影响，以期为福建烟区构建合理耕层及建立烤烟优质高效栽培体系提供科学依据。【方法】以福建烟区自然耕深15 cm+中耕培土为对照（CK），设耕深20 cm+中耕培土（D1）、耕深25 cm+中耕培土（D2）和耕深25 cm+免中耕培土（D3）3个深耕处理，对比分析各处理的烤烟产量产值及土壤理化性质差异，并采用Illumina MiSeq高通量测序技术，系统分析深耕处理下根际细菌结构差异与功能多样性变化，运用Spearman相关系数与随机森林模型评估根际细菌群落与土壤养分及烤烟产量的相关性。【结果】深耕可显著提高烤烟产量产值（P<0.05，下同），以D2处理增产增效效果最好，但与D3处理间差异不显著（P>0.05，下同）。土壤性质方面，各深耕处理均可较CK显著改善土壤总孔隙度和毛管孔隙度；土壤碱解氮含量及脲酶、蔗糖酶和过氧化氢酶活性随着耕深的加深而提高。适当深耕可显著提高细菌α多样性；深耕处理下绿弯菌门（Chloroflexi）与伯克氏菌属（Burkholderia）相对丰度显著上升，而罗河杆菌属（Rhodanobacter）相对丰度显著下降。Bugbase表型预测结果表明，深耕处理可显著提高根际土壤好氧菌与革兰氏阴性菌丰度，D2和D3处理较CK和D1处理显著提高氧化胁迫耐受菌相对丰度。FAPROTAX功能预测结果显示，各深耕处理均可较CK显著提升固氮作用（Nitrogen fixation）、纤维素分解（Cellulolsis）等养分循环方面功能相对丰度。随机森林模型预测结果显示，固氮作用是影响烤烟产量形成的最主要因素。【结论】在福建烟区耕层普遍变浅现状下，适度深耕（25 cm）对改善烤烟根际微生态环境有积极作用，但适度深耕下烤烟旺长前是否进行中耕培土对烤烟产量产值无显著影响。
Keyword ：
根际土壤 根际土壤 深耕 深耕 烤烟 烤烟 随机森林模型 随机森林模型 高通量测序 高通量测序
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| GB/T 7714 | 张汴泓 , 雷涵 , 李日坤 et al. 深耕对烤烟产量产值及根际土壤微环境的影响 [J]. | 南方农业学报 , 2023 , 54 (08) : 2195-2206 . |
| MLA | 张汴泓 et al. "深耕对烤烟产量产值及根际土壤微环境的影响" . | 南方农业学报 54 . 08 (2023) : 2195-2206 . |
| APA | 张汴泓 , 雷涵 , 李日坤 , 杨铭榆 , 潘睿欣 , 潘美清 et al. 深耕对烤烟产量产值及根际土壤微环境的影响 . | 南方农业学报 , 2023 , 54 (08) , 2195-2206 . |
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