Query：
学者姓名：庄伟建
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
The TEOSINTE-BRANCHED1/CYCLOIDEA/PROLIFERATING-CELL-FACTOR (TCP) gene family, a plant-specific transcription factor family, plays pivotal roles in various processes such as plant growth and development regulation, hormone crosstalk, and stress responses. However, a comprehensive genome-wide identification and characterization of the TCP gene family in peanut has yet to be fully elucidated. In this study, we conducted a genome-wide search and identified 51 TCP genes (designated as AhTCPs) in peanut, unevenly distributed across 17 chromosomes. These AhTCPs were phylogenetically classified into three subclasses: PCF, CIN, and CYC/TB1. Gene structure analysis of the AhTCPs revealed that most AhTCPs within the same subclade exhibited conserved motifs and domains, as well as similar gene structures. Cis-acting element analysis demonstrated that the AhTCP genes harbored numerous cis-acting elements associated with stress response, plant growth and development, plant hormone response, and light response. Intraspecific collinearity analysis unveiled significant collinear relationships among 32 pairs of these genes. Further collinear evolutionary analysis found that peanuts share 30 pairs, 24 pairs, 33 pairs, and 100 pairs of homologous genes with A. duranensis, A. ipaensis, Arabidopsis thaliana, and Glycine max, respectively. Moreover, we conducted a thorough analysis of the transcriptome expression profiles in peanuts across various tissues, under different hormone treatment conditions, in response to low- and high-calcium treatments, and under low-temperature and drought stress scenarios. The qRT-PCR results were in accordance with the transcriptome expression data. Collectively, these studies have established a solid theoretical foundation for further exploring the biological functions of the TCP gene family in peanuts, providing valuable insights into the regulatory mechanisms of plant growth, development, and stress responses.
Keyword ：
bioinformatics bioinformatics expression pattern analysis expression pattern analysis genome-wide identification genome-wide identification peanut peanut TCP gene TCP gene
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhu, Yanting , Niu, Sijie , Lin, Jingyi et al. Genome-Wide Identification and Expression Analysis of TCP Transcription Factors Responding to Multiple Stresses in Arachis hypogaea L. [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (3) . |
| MLA | Zhu, Yanting et al. "Genome-Wide Identification and Expression Analysis of TCP Transcription Factors Responding to Multiple Stresses in Arachis hypogaea L." . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 3 (2025) . |
| APA | Zhu, Yanting , Niu, Sijie , Lin, Jingyi , Yang, Hua , Zhou, Xun , Wang, Siwei et al. Genome-Wide Identification and Expression Analysis of TCP Transcription Factors Responding to Multiple Stresses in Arachis hypogaea L. . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明属于植物基因工程技术领域，具体公开了一种花生糖转运蛋白基因AhSWEET42及其应用。所述基因AhSWEET42的核苷酸序列如SEQ ID No.1所示，其编码蛋白的氨基酸序列如SEQ ID No.2所示。通过Gateway系统构建CaMV 35S启动子驱动的过量表达载体经农杆菌介导转化野生型拟南芥哥伦比亚零，通过对转基因植株进行分子检测和可溶性糖含量鉴定，其在拟南芥中超量表达可显著提高转基因拟南芥可溶性糖含量，表明AhSWEET42可能参与了植物甜卸载和糖积累反应。本发明为利用基因工程手段培育高甜花生等新品种提供了重要的基因资源，具有重要的应用前景。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 蔡铁城 , 潘益惊 , 熊发前 et al. 一种花生糖转运蛋白基因AhSWEET42及其应用 : CN202510550044.X[P]. | 2025-04-29 . |
| MLA | 蔡铁城 et al. "一种花生糖转运蛋白基因AhSWEET42及其应用" : CN202510550044.X. | 2025-04-29 . |
| APA | 蔡铁城 , 潘益惊 , 熊发前 , 庄伟建 , 陈浪 , 张冲 et al. 一种花生糖转运蛋白基因AhSWEET42及其应用 : CN202510550044.X. | 2025-04-29 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明属于植物基因工程技术领域，具体公开了一种花生糖转运蛋白基因AhSUT4及其在改善花生的味道和质量中的应用。所述基因AhSUT4的核苷酸序列如SEQ ID No.1所示，其编码蛋白的氨基酸序列如SEQ ID No.2所示。通过Gateway系统构建CaMV 35S启动子驱动的过量表达载体经农杆菌介导转化野生型拟南芥哥伦比亚零，通过对转基因植株进行分子检测和可溶性糖含量鉴定，其在拟南芥中超量表达可显著提高转基因拟南芥可溶性糖含量，表明AhSUT4可能参与了植物甜卸载和糖积累反应。本发明为利用基因工程手段培育高甜花生等新品种提供了重要的基因资源，具有重要的应用前景。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 蔡铁城 , 潘益惊 , 庄伟建 et al. 一种花生糖转运蛋白基因AhSUT4及其应用 : CN202510550148.0[P]. | 2025-04-29 . |
| MLA | 蔡铁城 et al. "一种花生糖转运蛋白基因AhSUT4及其应用" : CN202510550148.0. | 2025-04-29 . |
| APA | 蔡铁城 , 潘益惊 , 庄伟建 , 张冲 , 熊发前 , 陈浪 et al. 一种花生糖转运蛋白基因AhSUT4及其应用 : CN202510550148.0. | 2025-04-29 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明提供一种花生抗青枯病NBS‑LRR编码基因AhRRS6及其应用，所述基因AhRRS6的核苷酸序列如SEQ ID NO.1（来自抗病品种YY92的基因组）和SEQ ID NO.2（来自感病品种XHXL的基因组）所示。所述基因AhRRS6（SEQ ID NO.1）异源超表达烟草显著提高烟草对青枯病的抗性。通过构建AhRRS6的超表达载体转基因本氏烟草，在青枯菌侵染下，来自抗病品种的AhRRS6y与感病品种的AhRRS6x基因及非转基因植株相比，抗病品种转基因植株表现明显抗病性，而感病品种的等位基因和非转基因对照表现感病。此发明为植物抗青枯病的基因工程育种提供了基因资源和理论基础。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 庄伟建 , 付辉文 , 庄宇慧 et al. 一种花生抗青枯病NBS-LRR编码基因AhRRS6及其应用 : CN202510305464.1[P]. | 2025-03-14 . |
| MLA | 庄伟建 et al. "一种花生抗青枯病NBS-LRR编码基因AhRRS6及其应用" : CN202510305464.1. | 2025-03-14 . |
| APA | 庄伟建 , 付辉文 , 庄宇慧 , 张冲 , 陈华 , 蔡铁城 et al. 一种花生抗青枯病NBS-LRR编码基因AhRRS6及其应用 : CN202510305464.1. | 2025-03-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The SWEET (sugars will eventually be exported transporter) gene family represents a novel class of sugar transporters capable of bidirectionally transporting sugars along the concentration gradient. In this study, we identified 50 SWEET genes from the peanut cultivar Shitouqi, which were phylogenetically classified into four clades. Promoter analysis revealed that the AhSWEET genes contain multiple cis-acting elements associated with stress responses, growth regulation, and hormone signaling, suggesting their potential roles in plant development and adaptation to environmental challenges. Transcriptome profiling highlighted AhSWEET50 as the most highly expressed member during early seed development stages in both low- and high-sucrose peanut cultivars and also highly expressed at the mature stage. Subcellular localization confirmed the presence of AhSWEET50 in both the plasma membrane and cytoplasm, with predominant expression observed in embryos. The heterologous overexpression of AhSWEET50 in Arabidopsis significantly increased soluble sugar accumulation when compared to wild-type plants. These results validate the functional role of AhSWEET50 in sugar transport and provide a foundation for understanding the mechanisms of sugar allocation in peanuts, which has implications for improving seed quality through metabolic engineering.
Keyword ：
expression analysis expression analysis functional identification functional identification peanut peanut sweet sweet
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Cai, Tiecheng , Pan, Yijing , Zhang, Chong et al. Identification and Characterization of SWEET Gene Family in Peanuts and the Role of AhSWEET50 in Sugar Accumulation [J]. | AGRONOMY-BASEL , 2025 , 15 (5) . |
| MLA | Cai, Tiecheng et al. "Identification and Characterization of SWEET Gene Family in Peanuts and the Role of AhSWEET50 in Sugar Accumulation" . | AGRONOMY-BASEL 15 . 5 (2025) . |
| APA | Cai, Tiecheng , Pan, Yijing , Zhang, Chong , Chen, Lang , Ji, Biaojun , Yang, Qiang et al. Identification and Characterization of SWEET Gene Family in Peanuts and the Role of AhSWEET50 in Sugar Accumulation . | AGRONOMY-BASEL , 2025 , 15 (5) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Bacterial wilt caused by Ralstonia solanacearum is a devastating disease affecting a great many crops including peanut. The pathogen damages plants via secreting type & SHcy; effector proteins (T3Es) into hosts for pathogenicity. Here, we characterized RipAU was among the most toxic effectors as Delta RipAU completely lost its pathogenicity to peanuts. A serine residue of RipAU is the critical site for cell death. The RipAU targeted a subtilisin-like protease (AhSBT1.7) in peanut and both protein moved into nucleus. Heterotic expression of AhSBT1.7 in transgenic tobacco and Arabidopsis thaliana significantly improved the resistance to R. solanacearum. The enhanced resistance was linked with the upregulating ERF1 defense marker genes and decreasing pectin methylesterase (PME) activity like PME2&4 in cell wall pathways. The RipAU played toxic effect by repressing R-gene, defense hormone signaling, and AhSBTs metabolic pathways but increasing PMEs expressions. Furthermore, we discovered AhSBT1.7 interacted with AhPME4 and was colocalized at nucleus. The AhPME speeded plants susceptibility to pathogen via mediated cell wall degradation, which inhibited by AhSBT1.7 but upregulated by RipAU. Collectively, RipAU impaired AhSBT1.7 defense for pathogenicity by using PME-mediated cell wall degradation. This study reveals the mechanism of RipAU pathogenicity and AhSBT1.7 resistance, highlighting peanut immunity to bacterial wilt for future improvement.
Keyword ：
AhPME4 AhPME4 AhSBT1.7 AhSBT1.7 pathogenicity pathogenicity peanut peanut Ralstonia solanacearum Ralstonia solanacearum RipAU RipAU
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Kun , Zhuang, Yuhui , Chen, Hua et al. A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation [J]. | PLANT JOURNAL , 2025 , 121 (2) . |
| MLA | Chen, Kun et al. "A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation" . | PLANT JOURNAL 121 . 2 (2025) . |
| APA | Chen, Kun , Zhuang, Yuhui , Chen, Hua , Lei, Taijie , Li, Mengke , Wang, Shanshan et al. A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation . | PLANT JOURNAL , 2025 , 121 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了一种烟草抗青枯病基因NtRRS4及其在烟草抗青枯病中的应用，本方案基于前期烟草基因芯片数据筛选出的受青枯菌诱导上调表达的NBS‑LRR类抗病基因NtRRS4，经过PCR克隆获得编码该蛋白的cDNA序列，通过基于Gateway系统的BP和LR反应构建CaMV 35S启动子驱动NtRRS4基因植物表达载体p35S::NtRRS4‑TAP，将其转化GV3101农杆菌，通过叶盘法将其导入感病烟草品种红花大金元上，对转基因烟草进行分子鉴定和青枯菌接种抗性鉴定，结果证明NtRRS4可以正向调控烟草对青枯菌的抗性。本发明为利用基因工程手段培育抗青枯病烟草新品种提供了基因资源，具有重要的应用价值。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈华 , 陈顺辉 , 庄伟建 et al. 一种烟草抗青枯病基因NtRRS4及其在烟草抗青枯病中的应用 : CN202311770729.2[P]. | 2023-12-20 . |
| MLA | 陈华 et al. "一种烟草抗青枯病基因NtRRS4及其在烟草抗青枯病中的应用" : CN202311770729.2. | 2023-12-20 . |
| APA | 陈华 , 陈顺辉 , 庄伟建 , 王帅印 , 蔡铁城 , 张冲 et al. 一种烟草抗青枯病基因NtRRS4及其在烟草抗青枯病中的应用 : CN202311770729.2. | 2023-12-20 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Key messageIntegrating GAB methods with high-throughput phenotyping, genome editing, and speed breeding hold great potential in designing future smart peanut cultivars to meet market and food supply demands.AbstractCultivated peanut (Arachis hypogaea L.), a legume crop greatly valued for its nourishing food, cooking oil, and fodder, is extensively grown worldwide. Despite decades of classical breeding efforts, the actual on-farm yield of peanut remains below its potential productivity due to the complicated interplay of genotype, environment, and management factors, as well as their intricate interactions. Integrating modern genomics tools into crop breeding is necessary to fast-track breeding efficiency and rapid progress. When combined with speed breeding methods, this integration can substantially accelerate the breeding process, leading to faster access of improved varieties to farmers. Availability of high-quality reference genomes for wild diploid progenitors and cultivated peanuts has accelerated the process of gene/quantitative locus discovery, developing markers and genotyping assays as well as a few molecular breeding products with improved resistance and oil quality. The use of new breeding tools, e.g., genomic selection, haplotype-based breeding, speed breeding, high-throughput phenotyping, and genome editing, is probable to boost genetic gains in peanut. Moreover, renewed attention to efficient selection and exploitation of targeted genetic resources is also needed to design high-quality and high-yielding peanut cultivars with main adaptation attributes. In this context, the combination of genomics-assisted breeding (GAB), genome editing, and speed breeding hold great potential in designing future improved peanut cultivars to meet market and food supply demands.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Raza, Ali , Chen, Hua , Zhang, Chong et al. Designing future peanut: the power of genomics-assisted breeding [J]. | THEORETICAL AND APPLIED GENETICS , 2024 , 137 (3) . |
| MLA | Raza, Ali et al. "Designing future peanut: the power of genomics-assisted breeding" . | THEORETICAL AND APPLIED GENETICS 137 . 3 (2024) . |
| APA | Raza, Ali , Chen, Hua , Zhang, Chong , Zhuang, Yuhui , Sharif, Yasir , Cai, Tiecheng et al. Designing future peanut: the power of genomics-assisted breeding . | THEORETICAL AND APPLIED GENETICS , 2024 , 137 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Alternative splicing (AS), an important post-transcriptional regulation mechanism in eukaryotes, can significantly increase transcript diversity and contribute to gene expression regulation and many other complicated developmental processes. While plant gene AS events are well described, few studies have investigated the comprehensive regulation machinery of plant AS. Here, we use multi-omics to analyse peanut AS events. Using long-read isoform sequencing, 146 464 full-length non-chimeric transcripts were obtained, resulting in annotation corrections for 1782 genes and the identification of 4653 new loci. Using Iso-Seq RNA sequences, 271 776 unique splice junctions were identified, 82.49% of which were supported by transcriptome data. We characterized 50 977 polyadenylation sites for 23 262 genes, 12 369 of which had alternative polyadenylation sites. AS allows differential regulation of the same gene by miRNAs at the isoform level coupled with polyadenylation. In addition, we identified many long non-coding RNAs and fusion transcripts. There is a suppressed effect of 6mA on AS and gene expression. By analysis of chromatin structures, the genes located in the boundaries of topologically associated domains, proximal chromosomal telomere regions, inter- or intra-chromosomal loops were found to have more unique splice isoforms, higher expression, lower 6mA and more transposable elements (TEs) in their gene bodies than the other genes, indicating that chromatin interaction, 6mA and TEs play important roles in AS and gene expression. These results greatly refine the peanut genome annotation and contribute to the study of gene expression and regulation in peanuts. This work also showed AS is associated with multiple strategies for gene regulation.
Keyword ：
6mA 6mA alternative splicing alternative splicing chromatin structures chromatin structures Iso-Seq Iso-Seq peanut peanut
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wang, Lihui , Chen, Hua , Zhuang, Yuhui et al. Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut [J]. | PLANT BIOTECHNOLOGY JOURNAL , 2024 , 22 (6) : 1681-1702 . |
| MLA | Wang, Lihui et al. "Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut" . | PLANT BIOTECHNOLOGY JOURNAL 22 . 6 (2024) : 1681-1702 . |
| APA | Wang, Lihui , Chen, Hua , Zhuang, Yuhui , Chen, Kun , Zhang, Chong , Cai, Tiecheng et al. Multiple strategies, including 6mA methylation, affecting plant alternative splicing in allopolyploid peanut . | PLANT BIOTECHNOLOGY JOURNAL , 2024 , 22 (6) , 1681-1702 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The conidia produced by Fusarium oxysporum f. sp. cubense (Foc), the causative agent of Fusarium Wilt of Banana (FWB), play central roles in the disease cycle, as the pathogen lacks a sexual reproduction process. Until now, the molecular regulation network of asexual sporogenesis has not been clearly understood in Foc. Herein, we identified and functionally characterized thirteen (13) putative sporulation-responsive genes in Foc, namely FocmedA(a), FocmedA(b), abaA-L, FocflbA, FocflbB, FocflbC, FocflbD, FocstuA, FocveA, FocvelB, wetA-L, FocfluG and Foclae1. We demonstrated that FocmedA(a), abaA-L, wetA-L, FocflbA, FocflbD, FocstuA, FocveA and Foclae1 mediate conidiophore formation, whereas FocmedA(a) and abaA-L are important for phialide formation and conidiophore formation. The expression level of abaA-L was significantly decreased in the Delta FocmedA(a) mutant, and yeast one-hybrid and ChIP-qPCR analyses further confirmed that FocMedA(a) could bind to the promoter of abaA-L during micro- and macroconidiation. Moreover, the transcript abundance of the wetA-L gene was significantly reduced in the Delta abaA-L mutant, and it not only was found to function as an activator of micro- and macroconidium formation but also served as a repressor of chlamydospore production. In addition, the deletions of FocflbB, FocflbC, FocstuA and Foclae1 resulted in increased chlamydosporulation, whereas FocflbD and FocvelB gene deletions reduced chlamydosporulation. Furthermore, FocflbC, FocflbD, Foclae1 and FocmedA(a) were found to be important regulators for pathogenicity and fusaric acid synthesis in Foc. The present study therefore advances our understanding of the regulation pathways of the asexual development and functional interdependence of sporulation-responsive genes in Foc.
Keyword ：
asexual reproduction asexual reproduction Fusarium oxysporum f. sp. cubense Fusarium oxysporum f. sp. cubense genes genes sporogenesis regulation sporogenesis regulation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lu, Songmao , Deng, Huobing , Lin, Yaqi et al. A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense [J]. | JOURNAL OF FUNGI , 2024 , 10 (1) . |
| MLA | Lu, Songmao et al. "A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense" . | JOURNAL OF FUNGI 10 . 1 (2024) . |
| APA | Lu, Songmao , Deng, Huobing , Lin, Yaqi , Huang, Meimei , You, Haixia , Zhang, Yan et al. A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense . | JOURNAL OF FUNGI , 2024 , 10 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
