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学者姓名：郭玉春
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Abstract ：
马铃薯(Solanum tuberoum L.)作为全球第四大粮食作物,其高产稳产对保障粮食安全至关重要。近年来,耕地资源有限与病虫害压力促使生产者探索创新栽培技术。马铃薯/番茄嫁接体系因其可同步提高土地利用率与经济效益,成为研究热点。供试材料为紫色马铃薯"华颂66",番茄"金珠",试验设置自接[接穗和砧木均为紫色马铃薯"华颂66",即Potato/Potato(P/P)],嫁接[接穗为番茄"金珠",砧木为紫色马铃薯"华颂66",即Tomato/Potato(T/P)]两组处理,分别于嫁接后40 d(T1)、60 d(T2)和80 d(T3)采集块茎样本,结合Illumina NovaSeq 6000测序平台和Differential Expression analysis of Sequence data 2 (DESeq2)生物信息学分析,筛选差异基因,False discovery rate(FDR)<0.05,结果为:(1)无论是嫁接还是自接的情况,均是在生长80 d左右,块茎数目最多。嫁接后40 d,茎粗生长直径、叶片叶绿素含量和块茎数目上差异不明显,随着时间推移和植株生长,嫁接影响作用逐渐反馈在块茎生成上,嫁接后在相同的时间里,番茄嫁接马铃薯的单株块茎数明显增加,差异达到极显著水平,而叶绿素含量、块茎重差异不显著。(2)转组学分析结果表明,T1时差异表达基因(Differential expressed genes,DEGs)显著富集于微管结合(32基因,FDR=0.002)、液泡膜功能(45基因,FDR=0.005)及DNA复制(28基因,FDR=0.01)。糖酵解/糖异生(map00010)下调抑制淀粉合成。内质网蛋白质加工(map04141)上调增强蛋白折叠能力。植物激素信号转导(map04075),生长素响应因子ARF4(log2FC=-1.6)下调限制块茎膨大。嫁接通过多通路协同调控块茎发育:微管动态性降低:TUA3下调限制光合产物运输。糖酵解通量减少释放的ATP优先支持分生组织增长。HSP70上调缓解内质网应激,维持细胞稳态。通过多时间点转录组分析,揭示了嫁接紫色马铃薯块茎发育的分子调控框架:早期基因抑制主导(T1),中期代谢趋于稳定(T2),晚期分生组织效应显现(T3)。嫁接通过调控微管动态性、糖代谢重编程及胁迫响应通路,优先促进块茎分化而非膨大。未来将结合代谢组学与基因编辑技术,进一步...
Keyword ：
块茎生长 块茎生长 嫁接 嫁接 紫色马铃薯 紫色马铃薯 转录组学分析 转录组学分析
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| GB/T 7714 | 郭怡伶 , 符昌霖 , 何水林 et al. 转录组分析番茄嫁接紫色马铃薯对块茎生长的影响 [C] //第二十六届中国马铃薯大会 . 2025 . |
| MLA | 郭怡伶 et al. "转录组分析番茄嫁接紫色马铃薯对块茎生长的影响" 第二十六届中国马铃薯大会 . (2025) . |
| APA | 郭怡伶 , 符昌霖 , 何水林 , 郭玉春 , 张招娟 . 转录组分析番茄嫁接紫色马铃薯对块茎生长的影响 第二十六届中国马铃薯大会 . (2025) . |
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Abstract ：
马铃薯(Solanum tuberosum L.)作为全球第四大粮食作物，其高产稳产对保障粮食安全至关重要.近年来，耕地资源有限与病虫害压力促使生产者探索创新栽培技术.马铃薯/番茄嫁接体系因其可同步提高土地利用率与经济效益，成为研究热点.
Keyword ：
块茎生长 块茎生长 嫁接 嫁接 紫色马铃薯 紫色马铃薯 转录组学分析 转录组学分析
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| GB/T 7714 | 郭怡伶 , 符昌霖 , 何水林 et al. 转录组分析番茄嫁接紫色马铃薯对块茎生长的影响 [C] //2025年第二十六届中国马铃薯大会论文集 . 2025 : 272-272 . |
| MLA | 郭怡伶 et al. "转录组分析番茄嫁接紫色马铃薯对块茎生长的影响" 2025年第二十六届中国马铃薯大会论文集 . (2025) : 272-272 . |
| APA | 郭怡伶 , 符昌霖 , 何水林 , 郭玉春 , 张招娟 . 转录组分析番茄嫁接紫色马铃薯对块茎生长的影响 2025年第二十六届中国马铃薯大会论文集 . (2025) : 272-272 . |
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The normal metabolism of transient starch in leaves plays a vital role in determining photosynthesis and final crop yield. However, the molecular mechanisms linking abnormal transient starch metabolism to premature leaf senescence remain unclear. Here, we isolate a rice mutant, lses1, with leaf yellowing and premature senescence, as well as excessive accumulation of starch granules in chloroplasts. Genetic analysis revealed that this trait is controlled by a single recessive nuclear gene. Through BSA-seq preliminary gene mapping, map-based cloning, and sequencing alignment, the candidate gene was pinpointed to LOC_Os02g40860 on chromosome 2, which encodes OsCKI1, a casein kinase I family member. The identity of LSES1 was confirmed functionally: genetic complementation with the native genomic sequence rescued the wild-type phenotype, while CRISPR/Cas9 knockout of the gene in wild-type plants recapitulated the premature senescence. This confirmed that LSES1/OsCKI1 is involved in the regulation of leaf senescence. Notably, one improved knockout line, KO-2, displayed significant agronomic improvements in grain length, grain width, number of productive ears, and number of filled grains per panicle, along with a significant increase in grain yield per plant, highlighting its potential breeding value. Subcellular localization and tissue-specific expression analysis showed that LSES1 is primarily nuclear-localized and constitutively expressed.
Keyword ：
BSA-seq BSA-seq map-based cloning map-based cloning rice rice transitory starch transitory starch
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| GB/T 7714 | Chen, Fangyu , Zhang, Qishen , Wei, Xinyu et al. LSES1, Encoding a Member of the Casein Kinase 1 Family, Is Involved in the Regulation of Leaf Senescence in Rice [J]. | AGRONOMY-BASEL , 2025 , 15 (11) . |
| MLA | Chen, Fangyu et al. "LSES1, Encoding a Member of the Casein Kinase 1 Family, Is Involved in the Regulation of Leaf Senescence in Rice" . | AGRONOMY-BASEL 15 . 11 (2025) . |
| APA | Chen, Fangyu , Zhang, Qishen , Wei, Xinyu , Chen, Zhiming , Xu, Ming , Zhuang, Mancheng et al. LSES1, Encoding a Member of the Casein Kinase 1 Family, Is Involved in the Regulation of Leaf Senescence in Rice . | AGRONOMY-BASEL , 2025 , 15 (11) . |
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The biosynthesis of anthocyanins is influenced by external environmental conditions such as light, temperature, and nitrogen level, with nitrogen level being a key factor in anthocyanin synthesis and accumulation. Nitrogen level regulates the transcription factors involved in the anthocyanin synthesis pathway, with low nitrogen levels promoting anthocyanin accumulation, while high nitrogen levels have the opposite effect. Purple potatoes are a type of cultivated crop that is rich in anthocyanins and has unique economic value. Nitrogen fertilizer is crucial to improve the agronomic traits, yield, quality, and anthocyanin content of purple potatoes. In this study, the impact of four different nitrogen concentrations-0 kg/hm2 (N0), 90 kg/hm2 (N1), 225 kg/hm2 (N2) and 360 kg/hm2 (N3)-on the agronomic traits, yield, quality, and anthocyanin content of purple potatoes, 'Huasong 66', at different stages were investigated by using physiological index measurement and RNA-seq technology. It was found that the purple potato 'Huasong 66' was more sensitive to low nitrogen (N1). Under N1 level of nitrogen fertilization, 'Huasong 66' possessed the finest agronomic traits, yield, and quality, and the total anthocyanins in the tubers were significantly increased. Furthermore, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that nitrogen levels in purple potato tubers primarily affect genes related to nutrient transport and metabolism by regulating carbon and nitrogen metabolism, enzyme catalysis and binding, and signal transduction. In addition, nine candidate genes related to the anthocyanin synthesis pathway had been preliminarily screened. These results provide a basis to understand the impact of different nitrogen levels on the tuber yield and anthocyanin synthesis of purple potatoes.
Keyword ：
anthocyanin anthocyanin nitrogen fertilizer nitrogen fertilizer purple potato purple potato RNA-seq RNA-seq
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| GB/T 7714 | Zhang, Zhaojuan , Cai, Binbin , Guo, Yiling et al. The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes [J]. | AGRICULTURE-BASEL , 2024 , 14 (1) . |
| MLA | Zhang, Zhaojuan et al. "The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes" . | AGRICULTURE-BASEL 14 . 1 (2024) . |
| APA | Zhang, Zhaojuan , Cai, Binbin , Guo, Yiling , Na, Tiancang , Guo, Yuchun . The Impact of Different Nitrogen Levels on the Tuber Yield and Anthocyanin Synthesis of Purple Potatoes . | AGRICULTURE-BASEL , 2024 , 14 (1) . |
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分析三叶青叶片蓝光补光下酚类成分及基因表达谱的变化情况,筛选酚类代谢相关差异表达基因,为提高三叶青叶品质提供依据。以蓝光补光和可见光的三叶青叶片为研究材料,蓝光补光可以显著提高新绿原酸、绿原酸和3-O-香豆酰奎宁酸等酚类成分含量。转录组共获得102 949条unigenes,其中有14 564条差异表达unigenes。通过GO功能可分为52个亚类,而KEGG富集分析显示差异表达基因显著富集在苯丙烷生物合成、黄酮类生物合成和异黄酮生物合成等酚类代谢途径中。在三叶青酚类成分生物合成相关通路中共鉴别出63条差异表达基因,包含苯丙氨酸氨裂解酶(phenylalanine ammonia-lyase, PAL)、肉桂醇脱氢酶(cinnamyl-alcohol dehydrogenase, CAD)和黄酮醇合成酶(flavonol synthase, FLS)等19种关键酶。本研究丰富了三叶青的遗传数据信息,为解析蓝光促进三叶青酚类成分生物合成机制、开展三叶青分子育种奠定基础。
Keyword ：
三叶青 三叶青 差异表达 差异表达 蓝光补光 蓝光补光 转录组 转录组 酚类合成 酚类合成
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| GB/T 7714 | 徐惠龙 , 杨楠 , 洪玉燕 et al. 基于转录组测序的蓝光补光调控三叶青叶片酚类成分生物合成途径分析 [J]. | 药学学报 , 2024 , 59 (10) : 2864-2870 . |
| MLA | 徐惠龙 et al. "基于转录组测序的蓝光补光调控三叶青叶片酚类成分生物合成途径分析" . | 药学学报 59 . 10 (2024) : 2864-2870 . |
| APA | 徐惠龙 , 杨楠 , 洪玉燕 , 潘梦婷 , 郭玉春 , 范世明 et al. 基于转录组测序的蓝光补光调控三叶青叶片酚类成分生物合成途径分析 . | 药学学报 , 2024 , 59 (10) , 2864-2870 . |
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Background The normal metabolism of transitory starch in leaves plays an important role in ensuring photosynthesis, delaying senescence and maintaining high yield in crops. OsCKI1 (casein kinase I1) plays crucial regulatory roles in multiple important physiological processes, including root development, hormonal signaling and low temperature-treatment adaptive growth in rice; however, its potential role in regulating temporary starch metabolism or premature leaf senescence remains unclear. To reveal the molecular regulatory mechanism of OsCKI1 in rice leaves, physiological, transcriptomic and proteomic analyses of leaves of osckI1 allele mutant lses1 (leaf starch excess and senescence 1) and its wild-type varieties (WT) were performed. Results Phenotypic identification and physiological measurements showed that the lses1 mutant exhibited starch excess in the leaves and an obvious leaf tip withering phenotype as well as high ROS and MDA contents, low chlorophyll content and protective enzyme activities compared to WT. The correlation analyses between protein and mRNA abundance are weak or limited. However, the changes of several important genes related to carbohydrate metabolism and apoptosis at the mRNA and protein levels were consistent. The protein-protein interaction (PPI) network might play accessory roles in promoting premature senescence of lses1 leaves. Comprehensive transcriptomic and proteomic analysis indicated that multiple key genes/proteins related to starch and sugar metabolism, apoptosis and ABA signaling exhibited significant differential expression. Abnormal increase in temporary starch was highly correlated with the expression of starch biosynthesis-related genes, which might be the main factor that causes premature leaf senescence and changes in multiple metabolic levels in leaves of lses1. In addition, four proteins associated with ABA accumulation and signaling, and three CKI potential target proteins related to starch biosynthesis were up-regulated in the lses1 mutant, suggesting that LSES1 may affect temporary starch accumulation and premature leaf senescence through phosphorylation crosstalk ABA signaling and starch anabolic pathways. Conclusion The current study established the high correlation between the changes in physiological characteristics and mRNA and protein expression profiles in lses1 leaves, and emphasized the positive effect of excessive starch on accelerating premature leaf senescence. The expression patterns of genes/proteins related to starch biosynthesis and ABA signaling were analyzed via transcriptomes and proteomes, which provided a novel direction and research basis for the subsequent exploration of the regulation mechanism of temporary starch and apoptosis via LSES1/OsCKI1 in rice.
Keyword ：
) ) Leaf senescence Leaf senescence lses1 lses1 Proteome Proteome Rice (Oryza sativa L Rice (Oryza sativa L Transcriptome Transcriptome Transitory starch Transitory starch
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| GB/T 7714 | Chen, Zhiming , Wang, Yongsheng , Huang, Rongyu et al. Integration of transcriptomic and proteomic analyses reveals several levels of metabolic regulation in the excess starch and early senescent leaf mutant lses1 in rice [J]. | BMC PLANT BIOLOGY , 2022 , 22 (1) . |
| MLA | Chen, Zhiming et al. "Integration of transcriptomic and proteomic analyses reveals several levels of metabolic regulation in the excess starch and early senescent leaf mutant lses1 in rice" . | BMC PLANT BIOLOGY 22 . 1 (2022) . |
| APA | Chen, Zhiming , Wang, Yongsheng , Huang, Rongyu , Zhang, Zesen , Huang, Jinpeng , Yu, Feng et al. Integration of transcriptomic and proteomic analyses reveals several levels of metabolic regulation in the excess starch and early senescent leaf mutant lses1 in rice . | BMC PLANT BIOLOGY , 2022 , 22 (1) . |
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Arabidopsis Casparian strip membrane domain proteins (CASPs) form a transmembrane scaffold to recruit lignin biosynthetic enzymes for Casparian strip (CS) formation. Rice is a semi-aquatic plant with a more complex root structure than Arabidopsis to adapt its growing conditions, where the different deposition of lignin and suberin is crucial for adaptive responses. Here, we observed the structure of rice primary and small lateral roots (SLRs), particularly the deposition patterns of lignin and suberin in wild type and Oscasp1 mutants. We found that the appearance time and structure of CS in the roots of rice are different from those of Arabidopsis and observed suberin deposition in the sclerenchyma in wild type roots. Rice CASP1 is highly similar to AtCASPs, but its expression is concentrated in SLR tips and can be induced by salt stress especially in the steles. The loss of OsCASP1 function alters the expression of the genes involved in suberin biosynthesis and the deposition of suberin in the endodermis and sclerenchyma and leads to delayed CS formation and uneven lignin deposition in SLRs. These different depositions may alter nutrient uptake, resulting in ion imbalance in plant, withered leaves, fewer tillers, and reduced tolerance to salt stress. Our findings suggest that OsCASP1 could play an important role in nutrient homeostasis and adaptation to the growth environment.
Keyword ：
CASP CASP endodermis endodermis exodermis exodermis ion ion leaf senescence leaf senescence salt stress salt stress sclerenchyma sclerenchyma
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| GB/T 7714 | Yang, Xianfeng , Xie, Huifang , Weng, Qunqing et al. Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis [J]. | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
| MLA | Yang, Xianfeng et al. "Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis" . | FRONTIERS IN PLANT SCIENCE 13 (2022) . |
| APA | Yang, Xianfeng , Xie, Huifang , Weng, Qunqing , Liang, Kangjing , Zheng, Xiujuan , Guo, Yuchun et al. Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis . | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
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研究复合肥、缓释肥和土壤调节剂加复合肥3种不同肥料对紫色马铃薯生长特性及花青素含量的影响。以紫色马铃薯华颂66为供试材料,施用复合肥为对照,采用随机区组排列设计,利用HPLC法定性定量分析紫色马铃薯花青素含量。结果表明,施用缓释肥对植株高度、叶面积指数和产量具有显著的促进作用;同时显著提高维生素C和淀粉含量;但显著降低还原糖含量。花青素含量在土壤调节剂加复合肥的施用中最高,在缓释肥施用中含量最低。高效液相色谱分析表明,施用缓释肥时,总花青素组分中缺少天竺葵色素,推测该施肥种类对天竺葵色素合成具有抑制作用。因此,在生产实践上应根据不同目标需求,合理选用不同种类肥料。
Keyword ：
品质 品质 生长特性 生长特性 紫色马铃薯 紫色马铃薯 肥料 肥料 花青素 花青素
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| GB/T 7714 | 张招娟 , 黄家伟 , 黄泷健 et al. 施用不同种类肥料对紫色马铃薯生长、产量品质及花青素的影响 [J]. | 江苏农业科学 , 2021 , 49 (21) : 94-99 . |
| MLA | 张招娟 et al. "施用不同种类肥料对紫色马铃薯生长、产量品质及花青素的影响" . | 江苏农业科学 49 . 21 (2021) : 94-99 . |
| APA | 张招娟 , 黄家伟 , 黄泷健 , 郭玉春 . 施用不同种类肥料对紫色马铃薯生长、产量品质及花青素的影响 . | 江苏农业科学 , 2021 , 49 (21) , 94-99 . |
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本发明公开了一种高维生素C马铃薯酵素粉，属于食品加工技术领域。以提取马铃薯淀粉副产物马铃薯渣为主料，糯米、虫草粉、沙棘粉、混合酶菌种、蜂蜜为辅料，经过发酵得到高维生素C马铃薯酵素原液，再将原液制成固体粉末。本发明的高维生素C马铃薯酵素粉的制备方法简便快捷，成本低，其维生素C含量高，香气浓郁，令食欲增加，能够被人体迅速吸收，具有促进人体排毒、补气、恢复体力的作用，营养丰富，食用方便，没有任何副作用，是一种纯天然极高营养的健康食品。本发明为充分利用提取马铃薯淀粉后的副产物马铃薯渣的高附加值化利用提供新途径，亦为马铃薯深加工提供新思路，为国家推行马铃薯主食化找到了一条简便易行又物美价廉的理想途径。
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| GB/T 7714 | 张招娟 , 郭玉春 , 郭怡伶 et al. 一种高维生素C马铃薯酵素粉 : CN201911361816.6[P]. | 2019-12-26 . |
| MLA | 张招娟 et al. "一种高维生素C马铃薯酵素粉" : CN201911361816.6. | 2019-12-26 . |
| APA | 张招娟 , 郭玉春 , 郭怡伶 , 黄家伟 . 一种高维生素C马铃薯酵素粉 : CN201911361816.6. | 2019-12-26 . |
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福建省是中国南方重要的马铃薯冬作区,为明确福建省马铃薯主栽品种间的遗传差异,本试验利用SSR分子标记技术对19份主栽品种进行遗传多样性分析。结果表明,从分布于12对染色体的99对SSR引物中筛选出22对多态性高且条带清晰的引物,共扩增出207个等位位点,多态性比率为96.6%。每对SSR引物扩增的多态性位点数为7～22个,平均9个。19份主栽品种的遗传距离范围为0.338～0.925,平均值为0.594。聚类分析表明,在遗传距离0.724处可将所有供试材料分为A、B、C、D和E等5个类群,分别包含7份、5份、4份、1份和2份供试材料,说明供试材料具有较宽的遗传基础。研究表明,供试材料之间遗传多样性较高,为马铃薯品种知识产权保护和杂交育种提供理论依据。
Keyword ：
SSR SSR 指纹图谱 指纹图谱 遗传分析 遗传分析 马铃薯(Solanum tubersum L.) 马铃薯(Solanum tubersum L.)
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| GB/T 7714 | 张招娟 , 邓跃 , 黄泷健 et al. 福建省马铃薯主栽品种SSR遗传多样性分析 [J]. | 分子植物育种 , 2019 , 17 (22) : 7420-7427 . |
| MLA | 张招娟 et al. "福建省马铃薯主栽品种SSR遗传多样性分析" . | 分子植物育种 17 . 22 (2019) : 7420-7427 . |
| APA | 张招娟 , 邓跃 , 黄泷健 , 郭玉春 , 陈芳育 . 福建省马铃薯主栽品种SSR遗传多样性分析 . | 分子植物育种 , 2019 , 17 (22) , 7420-7427 . |
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