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学者姓名：陈选阳
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Abstract ：
China, the largest global producer of sweet potatoes, faces significant threats from viral diseases, particularly in Fujian Province, where sweet potatoes are the second most important food crop after rice. This study identified 11 viruses, including sweet potato feathery mottle virus (SPFMV) and sweet potato chlorotic stunt virus (SPCSV), infecting sweet potatoes in Fujian. Sequence comparisons revealed diverse strains from various sources. Virus prevalence varied across regions, with Quanzhou, Fuzhou, and Putian severely affected, detecting 10, 9, and 7 viruses, respectively, compared to only 3 in Sanming and Longyan. In particular, sweet potato virus disease (SPVD) caused the most severe damage during the seeding stages, resulting in dwarfing and leaf deformation, while the damage was lighter during the growth period, manifesting as the yellowing and brittleness of the leaves, ultimately reducing the yield. Compound infestations predominated, with between 0 and 6 viruses infecting different sweet potato varieties. Single-virus infections were observed for sweet potato virus 2 (SPV2), sweet potato symptomless virus 1 (SPSMV-1), and sweet potato pakakuy virus (SPPV), while others, particularly SPCSV, were frequently co-infected with SPFMV, leading to SPVD development. Further analysis showed that the RNase3 expression of SPCSV was correlated with the SPVD severity in sweet potato. These findings provide insights into the epidemiology of sweet potato viruses and serve as a reference for developing targeted disease management strategies.
Keyword ：
Fujian province Fujian province RNase3 RNase3 SPVD SPVD sweet potato sweet potato virus disease virus disease
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| GB/T 7714 | Zou, Weikun , Chen, Shi-Peng , Yang, Zhijian et al. Key Sweet Potato Viruses in Fujian Province and Their Distribution, Harmfulness, and Implications in China [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (4) . |
| MLA | Zou, Weikun et al. "Key Sweet Potato Viruses in Fujian Province and Their Distribution, Harmfulness, and Implications in China" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 47 . 4 (2025) . |
| APA | Zou, Weikun , Chen, Shi-Peng , Yang, Zhijian , Chen, Xuanyang . Key Sweet Potato Viruses in Fujian Province and Their Distribution, Harmfulness, and Implications in China . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2025 , 47 (4) . |
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Abstract ：
<正>甘薯作为中国重要的粮食作物，伴随着社会进步与人们生活方式及健康观念的转变，已逐渐成为当代的“宠儿”。随着甘薯产业的持续扩张与深化，其分工日趋精细，专业化程度亦在不断提升。特别是在福建省，甘薯作为首要的粮食与加工作物，其种植规模与产量均位列粮食作物前茅。因此，构建并完善福建省的甘薯产业社会化服务体系，对于推动甘薯产业的稳健发展，提升生产效益，促进农业结构的优化与调整，进而提升福建省农业的整体竞争力具有深远意义。社会化服务在农业领域具体表现在将生产、销售等各个环节外包给专业的社会化服务公司进行操作。农户只需将部分农业生产收益支付给这些公司，便可保留大部分收益。
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| GB/T 7714 | 黄尧烨 , 黄鸿文 , 乐奕 et al. 福建省甘薯产业社会化服务发展现状及探讨 [J]. | 世界热带农业信息 , 2025 , 7 (04) : 117-123 . |
| MLA | 黄尧烨 et al. "福建省甘薯产业社会化服务发展现状及探讨" . | 世界热带农业信息 7 . 04 (2025) : 117-123 . |
| APA | 黄尧烨 , 黄鸿文 , 乐奕 , 熊钒 , 陈选阳 , 杨志坚 . 福建省甘薯产业社会化服务发展现状及探讨 . | 世界热带农业信息 , 2025 , 7 (04) , 117-123 . |
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Researchers often need to synthesize genes of interest in this era of synthetic biology. Gene synthesis by PCR assembly of multiple DNA fragments is a quick and economical method that is widely applied. Up to now, there have been a few software solutions for designing fragments in gene synthesis. However, some of these software solutions use programming languages that are not popular now, other software products are commercial or require users to visit servers. In this study, we propose a Python program to design DNA fragments for gene synthesis. The algorithm is designed to meet the experimental needs. Also, the source code with detailed annotation is freely available for all users. Furthermore, the feasibility of the algorithm and the program is validated by experiments. Our program can be useful for the design of gene synthesis in the labs and help the study of gene structure and function.
Keyword ：
DNA fragment DNA fragment Gene synthesis Gene synthesis PCR PCR Python Python
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| GB/T 7714 | Hu, Yunzhuo , Pan, Danni , Xu, Fei et al. Gene synthesis design: a pythonic approach [J]. | PEERJ , 2024 , 12 . |
| MLA | Hu, Yunzhuo et al. "Gene synthesis design: a pythonic approach" . | PEERJ 12 (2024) . |
| APA | Hu, Yunzhuo , Pan, Danni , Xu, Fei , Huang, Bifang , Chen, Xuanyang , Lin, Shiqiang . Gene synthesis design: a pythonic approach . | PEERJ , 2024 , 12 . |
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Abstract ：
本实用新型涉及一种甘薯切条装置，包括机架，所述机架上设置有水平的作业台面，所述作业台面上设置有网格刀，所述网格刀的正上方设置有经电动机构驱动升降的压板，所述网格刀的外周部设置有甘薯的定位夹具。该甘薯切条装置设置有电动机构，电动机构能驱动压板升降。当把甘薯经定位夹具放置在网格刀上以后，电动机构会驱动压板下降，将甘薯向下压入网格刀，网格刀一次就能将甘薯切成条状。本实用新型采用电动推切的方式不仅省时省力，而且安全高效，使用方便。
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| GB/T 7714 | 黄尧烨 , 李珊 , 黄泽仪 et al. 一种甘薯切条装置 : CN202420508036.X[P]. | 2024-03-15 . |
| MLA | 黄尧烨 et al. "一种甘薯切条装置" : CN202420508036.X. | 2024-03-15 . |
| APA | 黄尧烨 , 李珊 , 黄泽仪 , 杨鸿 , 方浩宇 , 杨志坚 et al. 一种甘薯切条装置 : CN202420508036.X. | 2024-03-15 . |
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本发明公开了一种利用CRISPR‑Cas13a检测甘薯SPVD病毒病的方法，主要内容包括LwaCas13a基因的表达与蛋白纯化、guide RNA的获得、LwaCas13a‑sgRNA复合物组装、甘薯SPVD病毒病检测。主要原理是，构建LwaCas13a‑sgRNA复合物，利用其中的gRNA特异识别病毒RNA后，启动Cas13a剪切RNA的功能，释放荧光信号，经检测荧光信号，可快速检测低浓度的病毒RNA。该方法是继指示植物嫁接法、PCR法和ELISA三种甘薯病毒病检测方法之外的一种新方法，具有检测时间短、不需要逆转录、快速且高效的检测方法，检测的病毒浓度下限与PCR方法相当。
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| GB/T 7714 | 林世强 , 陈选阳 , 杨志坚 et al. 一种利用CRISPR-Cas13a检测甘薯SPVD病毒病的方法 : CN202411144569.5[P]. | 2024-08-20 . |
| MLA | 林世强 et al. "一种利用CRISPR-Cas13a检测甘薯SPVD病毒病的方法" : CN202411144569.5. | 2024-08-20 . |
| APA | 林世强 , 陈选阳 , 杨志坚 , 黄碧芳 , 许明 . 一种利用CRISPR-Cas13a检测甘薯SPVD病毒病的方法 : CN202411144569.5. | 2024-08-20 . |
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Gene cloning is an important step in investigating gene structure and function. To verify gene sequence, Sanger sequencing is used, which may produce several overlapping sequencing files that need to be merged before alignment to the target gene sequence is performed. Previously, we reported the Python program to Merge Sanger sequences (https://peerj.com/articles/11354/), which ran in command line and relied heavily on EMBOSS suite. In this updated version of the program, we have made several remarkable improvements. It provides a graphical user interface (GUI) written with tkinter, which is convenient and stable. It does not require users to rename the input sequences before performing merging. With regard to the implementation, the updated version utilizes Python function (Align.PairwiseAligner) to align adjacent sequences, which is more flexible (can adjust program parameter i.e., the number of first-time consecutive matching bases). The new version of the program makes merging Sanger sequences much more convenient and facilitates gene study.
Keyword ：
Gene cloning Gene cloning Merge Merge Python Python Sanger sequencing Sanger sequencing
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| GB/T 7714 | Lin, Shiming , Huang, Bifang , Zhao, Li-li et al. A Python program to merge Sanger sequences: an update [J]. | PEERJ , 2024 , 12 . |
| MLA | Lin, Shiming et al. "A Python program to merge Sanger sequences: an update" . | PEERJ 12 (2024) . |
| APA | Lin, Shiming , Huang, Bifang , Zhao, Li-li , Xu, Fei , Pan, Danni , Chen, Xuanyang et al. A Python program to merge Sanger sequences: an update . | PEERJ , 2024 , 12 . |
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本实用新型涉及一种操作简便的烤薯机，包括内部设有保温腔和烤制腔的箱体，保温腔与烤制腔之间通过甘薯输出口连通，烤制腔的内部上端设有若干个由旋转机构驱动旋转的烤筒，烤筒的底部设有由电动机构驱动上下摆转的密封盖；烤制腔的顶部设有若干个甘薯入口，若干个甘薯入口与若干个烤筒的位置相对应；烤制腔的内部下端设有用于呈接从若干个烤筒底部落下的甘薯的输送带，输送带的输出端与甘薯输出口相衔接，以将甘薯输送至保温腔。设计合理，甘薯受热均匀，烤制时间短，烤制结束后通过输送带将甘薯输送至保温腔，无需手动夹取，有效避免造成烫伤，操作方便；同时整体体积小，可放置在柜台上，能更好的适用于小型店铺，方便商家售卖，减少人与人之间的接触。
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| GB/T 7714 | 覃晓晖 , 杨志坚 , 陈选阳 et al. 一种操作简便的烤薯机 : CN202321349851.8[P]. | 2023-05-31 . |
| MLA | 覃晓晖 et al. "一种操作简便的烤薯机" : CN202321349851.8. | 2023-05-31 . |
| APA | 覃晓晖 , 杨志坚 , 陈选阳 , 陈义鑫 , 王楠 , 孙宜新 et al. 一种操作简便的烤薯机 : CN202321349851.8. | 2023-05-31 . |
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为推动甘薯薯圆的开发和利用，本文分析了薯圆的营养成分，并探究了其保健功能，研究表明薯圆中含有丰富的营养成分，具有抗氧化、调节血糖和血脂等功能，为薯圆的开发和利用提供借鉴。
Keyword ：
保健功能 保健功能 甘薯 甘薯 营养成分 营养成分 薯圆 薯圆
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| GB/T 7714 | 杨鸿 , 孔茗瑾 , 卢千柔 et al. 甘薯薯圆的营养成分及保健功效 [J]. | 安徽农学通报 , 2023 , 29 (22) : 122-124 . |
| MLA | 杨鸿 et al. "甘薯薯圆的营养成分及保健功效" . | 安徽农学通报 29 . 22 (2023) : 122-124 . |
| APA | 杨鸿 , 孔茗瑾 , 卢千柔 , 黄秋杰 , 杨志坚 , 陈选阳 . 甘薯薯圆的营养成分及保健功效 . | 安徽农学通报 , 2023 , 29 (22) , 122-124 . |
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本发明公开了一种富含花色苷的紫薯薯圆，其是以紫心甘薯新品种金薯20为主要原料，通过添加木薯粉、太白粉及甜叶菊制备获得。本发明从164个甘薯种质资源中筛选出适合制作薯圆的紫心甘薯品种金薯20，利用其为原料制备薯圆，并结合太白粉的使用，可在保持薯圆的口感及外观的同时，大大降低木薯粉的用量，从而提高薯圆食用的安全性及健康性。
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| GB/T 7714 | 杨志坚 , 陈选阳 , 陈凌华 . 一种富含花色苷的紫薯薯圆 : CN202310709392.8[P]. | 2023-06-15 . |
| MLA | 杨志坚 et al. "一种富含花色苷的紫薯薯圆" : CN202310709392.8. | 2023-06-15 . |
| APA | 杨志坚 , 陈选阳 , 陈凌华 . 一种富含花色苷的紫薯薯圆 : CN202310709392.8. | 2023-06-15 . |
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Red and blue light-emitting diodes (LEDs) affect the quality of sweet potato leaves and their nutritional profile. Vines cultivated under blue LEDs had higher soluble protein contents, total phenolic compounds, flavonoids, and total antioxidant activity. Conversely, chlorophyll, soluble sugar, protein, and vitamin C contents were higher in leaves grown under red LEDs. Red and blue light increased the accumulation of 77 and 18 metabolites, respectively. Alpha-linoleic and linolenic acid metabolism were the most significantly enriched pathways based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A total of 615 genes were differentially expressed between sweet potato leaves exposed to red and blue LEDs. Among these, 510 differentially expressed genes were upregulated in leaves grown under blue light compared with those grown under red light, while the remaining 105 genes were expressed at higher levels in the latter than in the former. Among the KEGG enrichment pathways, blue light significantly induced anthocyanin and carotenoid biosynthesis structural genes. This study provides a scientific reference basis for using light to alter metabolites to improve the quality of edible sweet potato leaves.
Keyword ：
anthocyanin anthocyanin illumination illumination leafy vegetable leafy vegetable metabolome profiling metabolome profiling nutrition nutrition
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| GB/T 7714 | Tadda, Shehu A. , Li, Chengyue , Ding, Jintao et al. Integrated metabolome and transcriptome analyses provide insight into the effect of red and blue LEDs on the quality of sweet potato leaves [J]. | FRONTIERS IN PLANT SCIENCE , 2023 , 14 . |
| MLA | Tadda, Shehu A. et al. "Integrated metabolome and transcriptome analyses provide insight into the effect of red and blue LEDs on the quality of sweet potato leaves" . | FRONTIERS IN PLANT SCIENCE 14 (2023) . |
| APA | Tadda, Shehu A. , Li, Chengyue , Ding, Jintao , Li, Jian'an , Wang, Jingjing , Huang, Huaxing et al. Integrated metabolome and transcriptome analyses provide insight into the effect of red and blue LEDs on the quality of sweet potato leaves . | FRONTIERS IN PLANT SCIENCE , 2023 , 14 . |
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