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学者姓名：黄碧光
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Abstract ：
A spontaneous barley mutant, young leaf chlorina (ylc), was identified in this study. Young leaves of ylc exhibited a yellow base and green tip, with reduced chlorophyll content, and altered chlorophyll fluorescence pattern, and underdeveloped grana in chloroplasts. The color of mutant leaves gradually transitioned to pale green and then became green in mature leaves. The ylc phenotype was found to be controlled by a recessive locus mapped to a 2.4 Mb interval on chromosome 7HS using bulked-segregant analysis with deep sequencing and further fine mapped to a 410 kb interval using polymorphic markers. The YLC locus co-segregated with a molecular marker that led to identification of HORVU7Hr1G011570 as the most likely candidate gene. As compared to the barley reference genome, the candidate ylc allele contained nucleotide changes that would lead to functional alterations of its protein product. The candidate YLC gene encodes a DYW-type pentatricopeptide repeat (PPR) protein, implicated in RNA cleavage and RNA editing in chloroplasts. Chlorophyll fluorescence analysis suggests that the PPR protein may regulate chloroplast development through the function of NAD(P)H dehydrogenase (NDH) complex and plays a pivotal role in mediating electron flow in thylakoid membranes during leaf growth in barley.
Keyword ：
Barley Barley BSA-seq BSA-seq Fine mapping Fine mapping PPR PPR RNA-seq RNA-seq Young leaf chlorina Young leaf chlorina
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| GB/T 7714 | Huang, Biguang , Huang, Daiqing , Zhang, Jianchun et al. Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves [J]. | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
| MLA | Huang, Biguang et al. "Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves" . | PLANT MOLECULAR BIOLOGY 115 . 2 (2025) . |
| APA | Huang, Biguang , Huang, Daiqing , Zhang, Jianchun , Xiong, Jiwei , Wu, Shiyu , Zheng, Xinrong et al. Barley young leaf chlorina, a putative pentatricopeptide repeat gene, is essential for chloroplast development in young leaves . | PLANT MOLECULAR BIOLOGY , 2025 , 115 (2) . |
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Objectives Hygroryza aristata, an aquatic plant native to Southeast Asia, shows a high degree of adaptability to aquatic environments. H. aristata, which belongs to the Oryzeae tribe and is closely related to rice (Oryza sativa), holds potential for crop improvement, particularly in flood tolerance. This study aimed to sequence and assemble the genome of H. aristata. Data description We assembled the genome of H. aristata using 31.91 Gb of Pacific Biosciences (PacBio) High-fidelity (HiFi) data and 22.36 Gb of ultra long Oxford Nanopore Technology (ONT) data, resulting in two gap-free haplotype genomes, hap1 (349.74 Mb) and hap2 (347.98 Mb), each with 12 chromosomes and 23 telomeres. The continuity of chromosomes was supported by High-throughput chromosome conformation capture (Hi-C) data. The assemblies demonstrated high completeness, with > 99.8% of coverage rates, 98.4% of Benchmarking Universal Single-Copy Orthologs (BUSCO) scores, and > 11.0 of Long Terminal Repeat Assembly Index (LAI) scores per haplotype. RNA sequencing (RNA-seq) data (176.06 Gb) of six tissues was generated for genome annotation, identifying 39,139 and 38,746 protein-coding genes in hap1 and hap2, respectively.
Keyword ：
Gap-free Gap-free Genome annotation Genome annotation Genome assembly Genome assembly Haplotype-resolved Haplotype-resolved Hi-C Hi-C Hygroryza aristata Hygroryza aristata PacBio HiFi PacBio HiFi RNA-seq RNA-seq Ultra long ONT Ultra long ONT
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| GB/T 7714 | Yang, Li-Yao , Huang, Li-Kun , Lin, Jin-Bin et al. Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata [J]. | BMC GENOMIC DATA , 2025 , 26 (1) . |
| MLA | Yang, Li-Yao et al. "Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata" . | BMC GENOMIC DATA 26 . 1 (2025) . |
| APA | Yang, Li-Yao , Huang, Li-Kun , Lin, Jin-Bin , Xu, Cun-Jing , Tang, Wei-Qi , Huang, Bi-Guang . Haplotype-resolved and gap-free genome of a floating aquatic plant from the Oryzeae tribe, Hygroryza aristata . | BMC GENOMIC DATA , 2025 , 26 (1) . |
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信息技术与教育深度融合为教学改革提供了契机，从而产生各种混合式教学模式。慕课是最为常见的线上教学平台，而慕课堂则是与慕课相配套的线下教学平台。BOPPPS是作为学生全方位参与的线下课堂教学模式。让多种教学模式在“青草药识别”生物类课程中有机结合，可形成特有的混合式教学模式。采用“三合一”混合式教学模式，以“青草药识别”在线开放课程为载体，利用慕课堂为工具，按照BOPPPS模式来开展实体课堂教学，并秉持以学生为主体的教学理念，实现了课前、课中和课后的紧密联系，提高了教学效果。同时，在教学过程中，对课程教学内容和课程体系进行改革，与课程思政有机结合，在潜移默化中培养学生植物分类科学素养，传承中医理念及爱国主义情怀。
Keyword ：
BOPPPS BOPPPS SPOC SPOC 慕课 慕课 慕课堂 慕课堂 混合式教学 混合式教学 青草药识别 青草药识别
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| GB/T 7714 | 黄碧光 , 叶炎芳 . 生物类课程“三合一”混合式教学模式探析 [J]. | 现代园艺 , 2023 , 46 (07) : 180-182 . |
| MLA | 黄碧光 et al. "生物类课程“三合一”混合式教学模式探析" . | 现代园艺 46 . 07 (2023) : 180-182 . |
| APA | 黄碧光 , 叶炎芳 . 生物类课程“三合一”混合式教学模式探析 . | 现代园艺 , 2023 , 46 (07) , 180-182 . |
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Barley awns are highly active in photosynthesis and account for 30-50% of grain weight in barley. They are diverse in length, ranging from long to awnless, and in shape from straight to hooded or crooked. Their diversity and importance have intrigued geneticists for several decades. A large collection of awnness mutants are available-over a dozen of them have been mapped on chromosomes and a few recently cloned. Different awnness genes interact with each other to produce diverse awn phenotypes. With the availability of the sequenced barley genome and application of new mapping and gene cloning strategies, it will now be possible to identify and clone more awnness genes. A better understanding of the genetic basis of awn diversity will greatly facilitate development of new barley cultivars with improved yield, adaptability and sustainability.
Keyword ：
awn awn barley barley gene mapping gene mapping genetic epistasis genetic epistasis morphology morphology pleiotropism pleiotropism
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| GB/T 7714 | Huang, Biguang , Wu, Weiren , Hong, Zonglie . Genetic Loci Underlying Awn Morphology in Barley [J]. | GENES , 2021 , 12 (10) . |
| MLA | Huang, Biguang et al. "Genetic Loci Underlying Awn Morphology in Barley" . | GENES 12 . 10 (2021) . |
| APA | Huang, Biguang , Wu, Weiren , Hong, Zonglie . Genetic Loci Underlying Awn Morphology in Barley . | GENES , 2021 , 12 (10) . |
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Awns are extending structures from lemmas in grasses and are very active in photosynthesis, contributing directly to the filling of the developing grain. Barley (Hordeum vulgare L.) awns are highly diverse in shape and length and are known to be controlled by multiple awn-related genes. The genetic effects of these genes on awn diversity and development in barley are multiplexed and include complementary effect, cumulative effect, duplicate effect, recessive epistasis, dominant epistasis, and inhibiting effect, each giving a unique modified Mendelian ratio of segregation. The complexity of gene interactions contributes to the awn diversity in barley. Excessive gene interactions create a challenging task for genetic mapping and specific strategies have to be developed for mapping genes with specific interactive effects. Awn gene interactions can occur at different levels of gene expression, from the transcription factor-mediated gene transcription to the regulation of enzymes and metabolic pathways. A better understanding of gene interactions will greatly facilitate deciphering the genetic mechanisms underlying barley awn diversity and development.
Keyword ：
awn awn barley barley gene interaction gene interaction gene mapping gene mapping transcription factor transcription factor
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| GB/T 7714 | Huang, Biguang , Wu, Weiren , Hong, Zonglie . Genetic Interactions of Awnness Genes in Barley [J]. | GENES , 2021 , 12 (4) . |
| MLA | Huang, Biguang et al. "Genetic Interactions of Awnness Genes in Barley" . | GENES 12 . 4 (2021) . |
| APA | Huang, Biguang , Wu, Weiren , Hong, Zonglie . Genetic Interactions of Awnness Genes in Barley . | GENES , 2021 , 12 (4) . |
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本发明提供了一种大麦发芽方法，属于农业种植技术领域。该方法包括以下步骤：(1)准备96孔板，用剪刀剪开每个孔的底部，大麦种子按顺序竖直播于孔内，种子胚端朝下放置；(2)将放好种子的96孔板放置于垫有滤纸的托盘内，滤纸下方浇水，至滤纸有水析出，从培养开始每两天换一次滤纸；(3)培养7天整齐的发芽出苗，能一棵棵苗分开，之后30天内随时移栽到大田。
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| GB/T 7714 | 黄碧光 , 吴明霞 . 一种大麦发芽方法 : CN202010319782.0[P]. | 2020-04-22 . |
| MLA | 黄碧光 et al. "一种大麦发芽方法" : CN202010319782.0. | 2020-04-22 . |
| APA | 黄碧光 , 吴明霞 . 一种大麦发芽方法 : CN202010319782.0. | 2020-04-22 . |
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本发明公开了一种缩短大麦生育期的方法，属于农业种植技术领域。该方法，包括以下步骤：(1)大麦种子放在培养皿湿润滤纸上置于培养箱内5℃发芽培养7天，光照周期为：19小时光照培养，光照强度3500 lux，5小时暗培养；发芽之后移植于72孔培养盘培养土中，每天于14℃光照培养19小时，光照强度3500 lux，10℃暗培养5小时；(2)在培养箱内共培养22‑33天，麦苗达到2叶期后移栽到大田；(3)经过50‑54天大田生长，达到抽穗阶段，抽穗后再经过34天，种子成熟。本发明的方法大麦总生育期117~124天，比对照组生育期明显缩短。
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| GB/T 7714 | 黄碧光 , 吴明霞 . 一种缩短大麦生育期的方法 : CN202010608746.6[P]. | 2020-06-30 . |
| MLA | 黄碧光 et al. "一种缩短大麦生育期的方法" : CN202010608746.6. | 2020-06-30 . |
| APA | 黄碧光 , 吴明霞 . 一种缩短大麦生育期的方法 : CN202010608746.6. | 2020-06-30 . |
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本发明提供一种大麦防杂草播种新方法，属于农业种植技术领域。该方法，包括以下步骤; (1)准备72孔培养盘置于接盘中；用细培养土铺满培养盘孔洞，用镊子或铅笔戳个小孔，然后将大麦种子或预先发芽的种子放进在72孔的小孔中，覆土，正常浇水保湿，先置于培养箱内培养10天发芽，每天于14℃光照培养19小时，光照强度3500Lux，于10℃暗培养5小时；(2)培养10天后麦苗达到1叶期，去掉接盘，将72孔培养盘放进整理好的有潮湿蓬松土壤的畦面上，苗的根系充分接触土壤，在苗的表面撒一层蓬松土，平时正常浇水保湿，至抽穗结实。
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| GB/T 7714 | 黄碧光 , 范永立 , 黄锦文 et al. 一种大麦防杂草播种方法 : CN202010319766.1[P]. | 2020-04-22 . |
| MLA | 黄碧光 et al. "一种大麦防杂草播种方法" : CN202010319766.1. | 2020-04-22 . |
| APA | 黄碧光 , 范永立 , 黄锦文 , 吴明霞 . 一种大麦防杂草播种方法 : CN202010319766.1. | 2020-04-22 . |
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Barley (Hordeum vulgare) awns contribute to grain yield, but the genetic basis of awn development remains largely unclear. Five barley lines differing in awn traits and row types were used to create four F-2 populations. Genetic analyses revealed that four pairs of genes were involved in awn development: A/a (awnless/awned), B/b (awnless/awned), H/h (hooded/straight), and L/l (long/short). Of these four loci, A, H and L functioned on both central rows (CR) and lateral rows (LR) of the barley spikes, while B exhibited effect only on LR. A and B had duplicate effects on LR, and both showed dominant epistasis to loci H and L, whereas H was epistatic to L. Meanwhile, A and B were found to be genetically linked, with a row-type locus V located between them. The genetic distances of A-V and B-V were estimated to be 9.6 and 7.7 cM, respectively. Literature search suggested that A, H and V may correspond to the reported Lks1, Kap1 and Vrs1, respectively, whereas B is a novel gene specifically controlling awn development on LR, designated as Lsa1 for lateral spikelet awnless 1. The only barley homolog of wheat awn inhibitor gene B1, HORVU2Hr1G077570, is a potential candidate of Lsa1.
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| GB/T 7714 | Huang, Biguang , Huang, Daiqing , Hong, Zonglie et al. Genetic analysis reveals four interacting loci underlying awn trait diversity in barley (Hordeum vulgare) [J]. | SCIENTIFIC REPORTS , 2020 , 10 (1) . |
| MLA | Huang, Biguang et al. "Genetic analysis reveals four interacting loci underlying awn trait diversity in barley (Hordeum vulgare)" . | SCIENTIFIC REPORTS 10 . 1 (2020) . |
| APA | Huang, Biguang , Huang, Daiqing , Hong, Zonglie , Owie, Swithin Omosuwa , Wu, Weiren . Genetic analysis reveals four interacting loci underlying awn trait diversity in barley (Hordeum vulgare) . | SCIENTIFIC REPORTS , 2020 , 10 (1) . |
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摘 要：为提高教学效果，微助教被探索应用于《青草药识别》课程混合式教学中，实现了课前、课中和课后的紧密联系。学生既能够对课程知识点进行课前预习，又能在课堂中将知识点系统化，再通过课后的单元小测等巩固课堂知识。同时，利用微助教课堂现场的互评功能，对学生的专题汇报进行互相评价，打通了学生以评促学的通道，体现了以学习为中心的教学理念。微助教的低门槛和强大的功能为该课程的混合式教学提供了便利。
Keyword ：
SPOC SPOC 关键词混合式教学模式 关键词混合式教学模式 微助教 微助教 青草药识别 青草药识别
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| GB/T 7714 | 黄碧光 , 范永立 , 黄锦文 et al. 微助教在混合式教学中的应用——以《青草药识别》SPOC课程为例 [J]. | 教育现代化 , 2020 , 7 (79) : 1-4 . |
| MLA | 黄碧光 et al. "微助教在混合式教学中的应用——以《青草药识别》SPOC课程为例" . | 教育现代化 7 . 79 (2020) : 1-4 . |
| APA | 黄碧光 , 范永立 , 黄锦文 , 陈冬梅 . 微助教在混合式教学中的应用——以《青草药识别》SPOC课程为例 . | 教育现代化 , 2020 , 7 (79) , 1-4 . |
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