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学者姓名：季彪俊
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Abstract ：
Rapeseed (Brassica napus) is a globally important oilseed crop whose yield and quality are frequently limited by weed competition. In recent years, there have been significant advances in our understanding of herbicide-resistance mechanisms in rapeseed and in the development of herbicide-resistant rapeseed germplasm. Here, we summarize the molecular mechanisms of resistance to three herbicides: glyphosate, glufosinate, and acetolactate synthase (ALS) inhibitors. We discuss progress in the identification of new resistance genes and the development of herbicide-resistant rapeseed germplasm, from the initial identification of natural mutants to artificial mutagenesis screening, introduction of exogenous resistance genes, and gene editing. In addition, we describe how synthetic biology and directed protein evolution will contribute to precision-breeding efforts in the near future. This is the first review to systematically integrate non-target resistance mechanisms and the potential applications of multi-omics and AI technologies for breeding of herbicide-resistant rapeseed, together with strategies for managing the risks associated with gene flow, the evolution of herbicide-resistant weeds, and the occurrence of volunteer plants resulting from deployment of herbicide-resistant rapeseed. By synthesizing current knowledge and future trends, this review provides guidance for safe, effective, and innovative approaches to the sustainable development of herbicide-resistant rapeseed.
Keyword ：
germplasm development germplasm development herbicide resistance herbicide resistance molecular mechanism molecular mechanism rapeseed rapeseed
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| GB/T 7714 | Liu, Decai , Yu, Shicheng , Ji, Biaojun et al. Molecular Mechanisms of Herbicide Resistance in Rapeseed: Current Status and Future Prospects for Resistant Germplasm Development [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (17) . |
| MLA | Liu, Decai et al. "Molecular Mechanisms of Herbicide Resistance in Rapeseed: Current Status and Future Prospects for Resistant Germplasm Development" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 17 (2025) . |
| APA | Liu, Decai , Yu, Shicheng , Ji, Biaojun , Peng, Qi , Gao, Jianqin , Zhang, Jiefu et al. Molecular Mechanisms of Herbicide Resistance in Rapeseed: Current Status and Future Prospects for Resistant Germplasm Development . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (17) . |
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为了解甲基磺酸乙酯(EMS)对油菜田间性状的影响，寻找EMS诱变的最佳处理条件，并研究油菜突变体的性状表现。以江西省农业科学院品种赣油杂8号(赣油杂8号)为试材，比较其在不同EMS浓度(0.50%、0.75%、1.00%、1.25%、1.50%)处理后大田的出苗率，并观察分析萌发后的农艺性状。结果发现，油菜种子萌发率随EMS浓度的增加而下降，在1.00%的EMS浓度下接近半致死率，可为以后的诱变提供借鉴。经EMS处理后的油菜，其田间的农艺性状存在丰富的变异，株高、分枝高度、角果长度等8个指标的变异系数为21.11%～47.04%,均值为32.82%,其中主花序有效角果数变异系数最高。建议选用具有植株较高、枝条高度较高、主花序的有效长度较长、主花序有效角果数较多、每角粒数数量较大这类影响全株产量的性状的油菜品系。
Keyword ：
农艺性状 农艺性状 油菜 油菜 甲基磺酸乙酯 甲基磺酸乙酯 诱变 诱变
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| GB/T 7714 | 刘猛 , 钟玉芳 , 邵晓琪 et al. 油菜EMS处理后田间表现多样性分析 [J]. | 安徽农业科学 , 2025 , 53 (12) : 16-19 . |
| MLA | 刘猛 et al. "油菜EMS处理后田间表现多样性分析" . | 安徽农业科学 53 . 12 (2025) : 16-19 . |
| APA | 刘猛 , 钟玉芳 , 邵晓琪 , 程思潜 , 陈源 , 叶绍飞 et al. 油菜EMS处理后田间表现多样性分析 . | 安徽农业科学 , 2025 , 53 (12) , 16-19 . |
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为了解PEG处理对福参种子萌发的影响，以福建野生福参为材料，采取0%(CK)、20%、40%、60%这4个体积分数，处理种子1d和3d。结果表明，随着PEG溶液体积分数增高，处理时间延长，对福参种子发芽生长有着明显的抑制作用，表现为发芽势、发芽率显著下降，根长、芽长缩短；当PEG体积分数为20%、处理时间为1 d时，种子发芽效果最好。
Keyword ：
发芽势 发芽势 发芽率 发芽率 福参 福参 聚乙二醇PEG 聚乙二醇PEG
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| GB/T 7714 | 李如焱 , 邵晓琪 , 叶绍飞 et al. 聚乙二醇PEG引发对福参种子发芽的影响 [J]. | 种子科技 , 2025 , 43 (04) : 48-50 . |
| MLA | 李如焱 et al. "聚乙二醇PEG引发对福参种子发芽的影响" . | 种子科技 43 . 04 (2025) : 48-50 . |
| APA | 李如焱 , 邵晓琪 , 叶绍飞 , 季彪俊 . 聚乙二醇PEG引发对福参种子发芽的影响 . | 种子科技 , 2025 , 43 (04) , 48-50 . |
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The SWEET (sugars will eventually be exported transporter) gene family represents a novel class of sugar transporters capable of bidirectionally transporting sugars along the concentration gradient. In this study, we identified 50 SWEET genes from the peanut cultivar Shitouqi, which were phylogenetically classified into four clades. Promoter analysis revealed that the AhSWEET genes contain multiple cis-acting elements associated with stress responses, growth regulation, and hormone signaling, suggesting their potential roles in plant development and adaptation to environmental challenges. Transcriptome profiling highlighted AhSWEET50 as the most highly expressed member during early seed development stages in both low- and high-sucrose peanut cultivars and also highly expressed at the mature stage. Subcellular localization confirmed the presence of AhSWEET50 in both the plasma membrane and cytoplasm, with predominant expression observed in embryos. The heterologous overexpression of AhSWEET50 in Arabidopsis significantly increased soluble sugar accumulation when compared to wild-type plants. These results validate the functional role of AhSWEET50 in sugar transport and provide a foundation for understanding the mechanisms of sugar allocation in peanuts, which has implications for improving seed quality through metabolic engineering.
Keyword ：
expression analysis expression analysis functional identification functional identification peanut peanut sweet sweet
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| GB/T 7714 | Cai, Tiecheng , Pan, Yijing , Zhang, Chong et al. Identification and Characterization of SWEET Gene Family in Peanuts and the Role of AhSWEET50 in Sugar Accumulation [J]. | AGRONOMY-BASEL , 2025 , 15 (5) . |
| MLA | Cai, Tiecheng et al. "Identification and Characterization of SWEET Gene Family in Peanuts and the Role of AhSWEET50 in Sugar Accumulation" . | AGRONOMY-BASEL 15 . 5 (2025) . |
| APA | Cai, Tiecheng , Pan, Yijing , Zhang, Chong , Chen, Lang , Ji, Biaojun , Yang, Qiang et al. Identification and Characterization of SWEET Gene Family in Peanuts and the Role of AhSWEET50 in Sugar Accumulation . | AGRONOMY-BASEL , 2025 , 15 (5) . |
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为改良浦城县酸性土壤、促进作物生长，以浦城长秆白菜为材料，研究施用3种不同的土壤调理剂对土壤的改善作用，以及对白菜产量和品质的影响。结果表明，在pH 4.8的酸性土壤中，3种土壤调理剂在3000 kg/hm
Keyword ：
pH pH 产量 产量 土壤调理剂 土壤调理剂 浦城县 浦城县 白菜 白菜 质量 质量 酸性土壤 酸性土壤
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| GB/T 7714 | 叶绍飞 , 刘猛 , 钟玉芳 et al. 不同土壤调理剂对浦城长秆白菜产量与品质的影响 [J]. | 中国农学通报 , 2025 , 41 (13) : 97-100 . |
| MLA | 叶绍飞 et al. "不同土壤调理剂对浦城长秆白菜产量与品质的影响" . | 中国农学通报 41 . 13 (2025) : 97-100 . |
| APA | 叶绍飞 , 刘猛 , 钟玉芳 , 程思潜 , 陈源 , 季彪俊 . 不同土壤调理剂对浦城长秆白菜产量与品质的影响 . | 中国农学通报 , 2025 , 41 (13) , 97-100 . |
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Bacterial wilt caused by Ralstonia solanacearum is a devastating disease affecting a great many crops including peanut. The pathogen damages plants via secreting type & SHcy; effector proteins (T3Es) into hosts for pathogenicity. Here, we characterized RipAU was among the most toxic effectors as Delta RipAU completely lost its pathogenicity to peanuts. A serine residue of RipAU is the critical site for cell death. The RipAU targeted a subtilisin-like protease (AhSBT1.7) in peanut and both protein moved into nucleus. Heterotic expression of AhSBT1.7 in transgenic tobacco and Arabidopsis thaliana significantly improved the resistance to R. solanacearum. The enhanced resistance was linked with the upregulating ERF1 defense marker genes and decreasing pectin methylesterase (PME) activity like PME2&4 in cell wall pathways. The RipAU played toxic effect by repressing R-gene, defense hormone signaling, and AhSBTs metabolic pathways but increasing PMEs expressions. Furthermore, we discovered AhSBT1.7 interacted with AhPME4 and was colocalized at nucleus. The AhPME speeded plants susceptibility to pathogen via mediated cell wall degradation, which inhibited by AhSBT1.7 but upregulated by RipAU. Collectively, RipAU impaired AhSBT1.7 defense for pathogenicity by using PME-mediated cell wall degradation. This study reveals the mechanism of RipAU pathogenicity and AhSBT1.7 resistance, highlighting peanut immunity to bacterial wilt for future improvement.
Keyword ：
AhPME4 AhPME4 AhSBT1.7 AhSBT1.7 pathogenicity pathogenicity peanut peanut Ralstonia solanacearum Ralstonia solanacearum RipAU RipAU
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| GB/T 7714 | Chen, Kun , Zhuang, Yuhui , Chen, Hua et al. A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation [J]. | PLANT JOURNAL , 2025 , 121 (2) . |
| MLA | Chen, Kun et al. "A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation" . | PLANT JOURNAL 121 . 2 (2025) . |
| APA | Chen, Kun , Zhuang, Yuhui , Chen, Hua , Lei, Taijie , Li, Mengke , Wang, Shanshan et al. A Ralstonia effector RipAU impairs peanut AhSBT1.7 immunity for pathogenicity via AhPME-mediated cell wall degradation . | PLANT JOURNAL , 2025 , 121 (2) . |
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本发明提供了一种蝉花虫草孢子粉的生产方法，属于菌类栽培技术领域。本发明提供的一种蝉花虫草孢子粉的生产方法，包括如下步骤：(1)在PDA基础培养基的基础上添加大豆蛋白胨和饴糖，得到改良的PDA培养基；(2)在所述改良的PDA培养基中接种蝉花虫草菌种，进行培养；(3)培养结束后，收集蝉花虫草孢子粉。本发明提供的蝉花虫草孢子粉的生产方法，以改良PDA培养基为栽培基质，提高了蝉花虫草孢子粉的产量，且生产工艺简单，操作方便，周期短，经济效益高，具有广阔的应用价值。
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| GB/T 7714 | 季彪俊 , 邵晓琪 , 季宸羽 et al. 一种蝉花虫草孢子粉的生产方法 : CN202411659706.9[P]. | 2024-11-20 . |
| MLA | 季彪俊 et al. "一种蝉花虫草孢子粉的生产方法" : CN202411659706.9. | 2024-11-20 . |
| APA | 季彪俊 , 邵晓琪 , 季宸羽 , 张绪璋 , 胡佳仪 , 何可欣 et al. 一种蝉花虫草孢子粉的生产方法 : CN202411659706.9. | 2024-11-20 . |
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甘蓝型油菜是中国重要的油料作物，籽粒质量是油菜产量构成的重要因素。本研究利用A-D Test模型对496份油菜的籽粒质量进行全基因组关联分析，共检测到19个显著位点，联合解释34.1%的表型变异。整合A-D Test及前期混合线性模型和一般线性模型结果后得到71个位点，联合解释50.1%的表型变异。在22个位点置信区间内找到ARF2、NPC6、TTG2和WRI1等已被报道的拟南芥中的籽粒质量基因的同源基因。同时，利用大粒品种中双11和中小粒品种中油821的籽粒和角果皮转录组数据进行加权基因共表达网络分析，构建了13个共表达模块，其中紫色(purple)和洋红色(magenta)模块与籽粒质量表型显著相关。GO富集分析结果表明，2个模块在L-苯丙氨酸氨基转移酶活性、硫双加氧酶活性、焦磷酸酶活性和RNA解旋酶活性等显著富集。2个模块中的枢纽基因BnaA06g00850D、BnaA01g00990D、BnaC06g10000D和BnaC02g44260D等的同源基因为ETHE1、DAR1、GLN1;1和SMG7等拟南芥籽粒质量已知基因。整合全基因组关联分析和加权基因共表达网络分析的分析结果，在42个显著位点的置信区间内找到90个属于purple和magenta模块的基因，其中BnaA01g06210D、BnaA07g14990D和BnaA07g03030D等拟南芥同源基因已被报道参与种子发育进程调控。本研究整合GWAS与WGCNA 2种分析方法，挖掘甘蓝型油菜的籽粒质量候选基因，为研究籽粒质量的调控机制、指导籽粒质量的遗传改良提供参考。
Keyword ：
产量 产量 全基因组关联分析 全基因组关联分析 共表达网络分析 共表达网络分析 甘蓝型油菜 甘蓝型油菜 籽粒质量 籽粒质量
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| GB/T 7714 | 陈元军 , 马娟娟 , 史睿 et al. 整合关联分析和共表达网络分析挖掘甘蓝型油菜籽粒质量候选基因 [J]. | 江苏农业学报 , 2023 , 39 (04) : 913-930 . |
| MLA | 陈元军 et al. "整合关联分析和共表达网络分析挖掘甘蓝型油菜籽粒质量候选基因" . | 江苏农业学报 39 . 04 (2023) : 913-930 . |
| APA | 陈元军 , 马娟娟 , 史睿 , 李伟龙 , 王婷 , 彭琦 et al. 整合关联分析和共表达网络分析挖掘甘蓝型油菜籽粒质量候选基因 . | 江苏农业学报 , 2023 , 39 (04) , 913-930 . |
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甘蓝型油菜（Brassica napus L.）是我国重要的油料作物之一，提高甘蓝型油菜种子的含油量是育种工作的目标。利用转录组测序技术（RNA-seq）对甘蓝型油菜“中9”不同发育时期的种子样品进行表达分析，重点关注进行油脂合成的相关基因的动态表达分析，明确这些基因在种子发育不同时期的表达情况，为进一步阐释油脂合成相关基因代谢调控机理提供素材。
Keyword ：
油脂合成 油脂合成 甘蓝型油菜 甘蓝型油菜 种子发育 种子发育 转录组 转录组
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| GB/T 7714 | 王衍坤 , 沈怡 , 范佳琪 et al. 甘蓝型油菜油脂合成相关基因的动态表达分析 [J]. | 种子科技 , 2022 , 40 (12) : 1-3,39 . |
| MLA | 王衍坤 et al. "甘蓝型油菜油脂合成相关基因的动态表达分析" . | 种子科技 40 . 12 (2022) : 1-3,39 . |
| APA | 王衍坤 , 沈怡 , 范佳琪 , 陈思旭 , 夏法刚 , 季彪俊 . 甘蓝型油菜油脂合成相关基因的动态表达分析 . | 种子科技 , 2022 , 40 (12) , 1-3,39 . |
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[目的]了解12份水稻恢复系之间的遗传亲缘关系，以期为水稻恢复系选育和杂交组合选配提供分子依据。[方法]利用40对SSR引物对8份‘明恢’系列恢复系和4份种植广泛的恢复系进行遗传多样性研究。构建该12份恢复系的指纹图谱，并以标记数据为基础计算遗传距离和生成聚类图。[结果](1)40对SSR引物共检测出75个等位位点，平均每对引物产生1.875个；以其中多态性良好的11对标记对12份恢复系进行指纹图谱构建，确定了每份材料的唯一分子身份信息。(2)12份恢复系之间的遗传相似系数变异范围在0.62～0.93之间。在遗传相似系数0.74处可以将12份材料分为6个类群，在0.79处可以将第Ⅰ类群的6份材料分为3个亚类。[结论]12份供试恢复系可以分成6个亚群，明确了相互之间的亲缘关系，从分子层面为恢复系选育和亲本组配提供了较好的遗传信息。
Keyword ：
SSR分子标记 SSR分子标记 恢复系 恢复系 指纹图谱 指纹图谱 水稻 水稻 遗传多样性 遗传多样性
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| GB/T 7714 | 王衍坤 , 韦新宇 , 胡杰 et al. 12份水稻恢复系的遗传多样性分析及指纹图谱构建 [J]. | 亚热带农业研究 , 2022 , 18 (02) : 73-78 . |
| MLA | 王衍坤 et al. "12份水稻恢复系的遗传多样性分析及指纹图谱构建" . | 亚热带农业研究 18 . 02 (2022) : 73-78 . |
| APA | 王衍坤 , 韦新宇 , 胡杰 , 夏法刚 , 周元昌 , 季彪俊 . 12份水稻恢复系的遗传多样性分析及指纹图谱构建 . | 亚热带农业研究 , 2022 , 18 (02) , 73-78 . |
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