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学者姓名：兰涛
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Abstract ：
BackgroundDNA demethylases regulate the levels of genomic DNA methylation in plants. The demethylase REPRESSOR OF SILENCING 1 (ROS1) is a crucial factor for modulating gene expression in plant disease responses. Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. Oryzicola (Xoc), is a highly destructive disease in rice. BLS resistance in rice is known to be quantitatively inherited, but the mechanisms by which DNA methylation controls BLS resistance remain poorly understood.ResultsIn this study, we knocked down OsROS1a expression in the rice variety Nipponbare using RNA interference (RNAi). The average BLS lesion length in the transgenic (T2) OsROS1a-RNAi (RS) lines was significantly reduced compared to that in wild-type Nipponbare plants (NP). Using whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq), we analyzed the DNA methylations and transcriptomes of RS lines in comparison with NP at 0 (control), 5, 10, and 24 h post-inoculation with Xoc. A total of 1080 differentially expressed genes (DEGs) related to Xoc infection between the NP and RS lines were identified, which could be grouped into 8 clusters by K-means analysis. The DEGs in cluster 1 were enriched in the biological process related to defense response, response to stress, oxidation-reduction, etc. Integration of the methylome and transcriptome data revealed 112 overlapping differentially methylated and expressed genes (DMEGs). Gene Ontology (GO) analysis showed that the DMEGs were mainly involved in biological processes, such as metabolic process, cellular process, responses to stimulus, signaling, and immune system processes. KEGG pathway enrichment analysis revealed that these DMEGs were enriched in pathways related to glutathione metabolism, plant-pathogen interaction, cysteine and methionine, diterpenoid biosynthesis, photosynthesis, and starch and sucrose. Additionally, LOC_Os09g12660, encoding the glucose-1-phosphate adenylyl transferase large subunit, a chloroplast precursor involved in synthesizing activated glycosyl donor, showed strong potential to contribute to BLS resistance.ConclusionOsROS1a plays a crucial role in modulating rice resistance to bacterial leaf streak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc). These findings provide valuable insights into the role of OsROS1a in BLS resistance.
Keyword ：
Bacterial leaf streak Bacterial leaf streak Methylation Methylation OsROS1a OsROS1a Rice Rice Transcriptome Transcriptome
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| GB/T 7714 | Xie, Xiaofang , Yang, Xuansong , Lu, Libin et al. Exploring the molecular mechanism of OsROS1a in regulating resistance to bacterial leaf streak through transcriptome and DNA methylation profiling in rice (Oryza sativa L.) [J]. | BMC GENOMICS , 2025 , 26 (1) . |
| MLA | Xie, Xiaofang et al. "Exploring the molecular mechanism of OsROS1a in regulating resistance to bacterial leaf streak through transcriptome and DNA methylation profiling in rice (Oryza sativa L.)" . | BMC GENOMICS 26 . 1 (2025) . |
| APA | Xie, Xiaofang , Yang, Xuansong , Lu, Libin , Li, Tong , Qin, Mingyue , Guan, Huazhong et al. Exploring the molecular mechanism of OsROS1a in regulating resistance to bacterial leaf streak through transcriptome and DNA methylation profiling in rice (Oryza sativa L.) . | BMC GENOMICS , 2025 , 26 (1) . |
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Soil salinity severely inhibits rice growth. While the SBP-box gene OsSPL10 is known to negatively regulate salt tolerance, its mechanism remains unclear. Since jasmonate (JA) mediates plant stress responses, we investigated the link between OsSPL10 and JA in rice salt tolerance. Disrupting OsSPL10 reduced the shoot Na+/K+ ratio under salt stress, likely explaining the enhanced tolerance in Osspl10 mutants. As leaves are highly sensitive to Na+ toxicity, maintaining a low Na+/K+ ratio is critical for survival. Notably, ten OsJAZ genes (JA repressors) were upregulated in Osspl10 mutants. We found that OsSPL10 directly suppresses certain OsJAZ genes (including OsJAZ9, known to regulate certain ion transporters), by binding to their promoters. These findings suggest that OsSPL10 negatively modulate salt tolerance via the OsJAZ-mediated JA pathway, maintaining ion homeostasis. This study reveals new molecular mechanisms of rice salinity response, emphasizing the interplay between transcription factors and hormonal signaling.
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| GB/T 7714 | Chen, Jilin , Lan, Tong , Dong, Tongtong et al. OsSPL10 negatively regulates seedling salt tolerance through OsJAZ-mediated jasmonate pathway in rice (Oryza sativa L.) [J]. | THEORETICAL AND APPLIED GENETICS , 2025 , 138 (11) . |
| MLA | Chen, Jilin et al. "OsSPL10 negatively regulates seedling salt tolerance through OsJAZ-mediated jasmonate pathway in rice (Oryza sativa L.)" . | THEORETICAL AND APPLIED GENETICS 138 . 11 (2025) . |
| APA | Chen, Jilin , Lan, Tong , Dong, Tongtong , Liao, Qiaoling , An, Xiaoxiao , Fang, Chao et al. OsSPL10 negatively regulates seedling salt tolerance through OsJAZ-mediated jasmonate pathway in rice (Oryza sativa L.) . | THEORETICAL AND APPLIED GENETICS , 2025 , 138 (11) . |
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Drought tolerance and plant growth are critical factors affecting rice yield, and identifying genes that can enhance these traits is essential for improving crop resilience and productivity. Using a growth-depressed and drought-tolerant (gddt) mutant of the indica rice variety Huanghuazhan (HHZ) generated by radiation mutagenesis, we discovered a novel gene, GDDT, which plays a dual role in plant biology: it acts as a positive regulator of growth and development, but as a negative regulator of drought resistance. The gddt mutant displayed a marked reduction in plant growth and seed setting rate, yet exhibited an unexpected advantage in terms of drought tolerance. Our research revealed that the enhanced drought tolerance of the gddt mutant is primarily due to a decrease in stomatal size, density, and aperture, which reduces water loss, and an activation of the reactive oxygen species (ROS) scavenging system, which helps protect the plant from oxidative stress. These physiological changes are observed both under drought conditions and in normal growth conditions. This discovery highlights the importance of GDDT as a pleiotropic gene with significant implications for both plant growth and drought resistance. Through map-based cloning, we determined that the protein disulfide isomerase-like (PDIL) gene OsPDIL1-5 is the GDDT gene. The protein encoded by this gene was localized to the endoplasmic reticulum, consistent with its predicted function. Our findings provide new insights into the role of PDIL genes in rice and suggest that further study of GDDT could lead to a better understanding of how these genes contribute to the complex interplay between plant growth, development, and stress responses. This knowledge could pave the way for the development of rice varieties that are more resilient to drought, thereby increasing crop yields and ensuring food security in water-limited environments.
Keyword ：
drought tolerance drought tolerance GDDT GDDT growth and development growth and development OsPDIL1-5 OsPDIL1-5 rice rice
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| GB/T 7714 | Chen, Jilin , Zhang, Jin , Fang, Chao et al. OsPDIL1-5: dual role in promoting growth and development while modulating drought stress tolerance in rice (Oryza sativa L.) [J]. | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
| MLA | Chen, Jilin et al. "OsPDIL1-5: dual role in promoting growth and development while modulating drought stress tolerance in rice (Oryza sativa L.)" . | FRONTIERS IN PLANT SCIENCE 15 (2024) . |
| APA | Chen, Jilin , Zhang, Jin , Fang, Chao , Ren, Lijun , Lan, Tong , Wu, Weiren et al. OsPDIL1-5: dual role in promoting growth and development while modulating drought stress tolerance in rice (Oryza sativa L.) . | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
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Transcription factors (TFs) play essential roles in transcriptional reprogramming during activation of plant immune responses to pathogens. OsSPL10 (SQUAMOSA promoter binding protein-like10) is an important TF regulating trichome development and salt tolerance in rice. Here we report that knockout of OsSPL10 reduces whereas its overexpression enhances rice resistance to blast disease. OsSPL10 positively regulates chitin-induced immune responses including reactive oxygen species (ROS) burst and callose deposition. We show that OsSPL10 physically associates with OsJAmyb, an important TF involved in jasmonic acid (JA) signaling, and positively regulates its protein stability. We then prove that OsJAmyb positively regulates resistance to blast. Our results reveal a molecular module consisting of OsSPL10 and OsJAmyb that positively regulates blast resistance. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC
Keyword ：
Immunity Immunity Jasmonate Jasmonate Oryza sativa Oryza sativa OsSPL10 OsSPL10 Transcription factor Transcription factor
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| GB/T 7714 | Zhong, Zaofa , Zhong, Lijing , Zhu, Xiang et al. Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice [J]. | CROP JOURNAL , 2024 , 12 (1) : 301-307 . |
| MLA | Zhong, Zaofa et al. "Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice" . | CROP JOURNAL 12 . 1 (2024) : 301-307 . |
| APA | Zhong, Zaofa , Zhong, Lijing , Zhu, Xiang , Jiang, Yimin , Zheng, Yihong , Lan, Tao et al. Transcription factor OsSPL10 interacts with OsJAmyb to regulate blast resistance in rice . | CROP JOURNAL , 2024 , 12 (1) , 301-307 . |
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The small heat shock proteins (sHSPs) are important components in plant growth and development, and stress response. However, a systematical understanding of the sHSP family is yet to be reported in five diploid Gossypium species. In this study, 34 GlsHSPs, 36 GrsHSPs, 37 GtsHSPs, 37 GasHSPs, and 38 GhesHSPs were identified in Gossypium longicalyx, Gossypium raimondii, Gossypium turneri, Gossypium arboreum, and Gossypium herbaceum, respectively. These sHSP members can be clustered into 10 subfamilies. Different subfamilies had different member numbers, motif distributions, gene structures, gene duplication events, gene loss numbers, and cis-regulatory elements. Besides, the paleohexaploidization event in cotton ancestor led to expanding the sHSP members and it was also inherited by five diploid Gossypium species. After the cotton ancestor divergence, the sHSP members had the relatively conserved evolution in five diploid Gossypium species. The comprehensive evolutionary history of the sHSP family was revealed in five diploid Gossypium species. Furthermore, several GasHSPs and GhesHSPs were important candidates in plant growth and development, and stress response. These current findings can provide valuable information for the molecular evolution and further functional research of the sHSP family in cotton.
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| GB/T 7714 | Fan, Kai , Qian, Zhengyi , He, Yuxi et al. Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species [J]. | PLANT GENOME , 2024 , 17 (3) . |
| MLA | Fan, Kai et al. "Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species" . | PLANT GENOME 17 . 3 (2024) . |
| APA | Fan, Kai , Qian, Zhengyi , He, Yuxi , Chen, Jiayuan , Ye, Fangting , Zhu, Xiaogang et al. Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species . | PLANT GENOME , 2024 , 17 (3) . |
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Key messageOsSPL10 is a negative regulator of rice defense against BPH, knockout of OsSPL10 enhances BPH resistance through upregulation of defense-related genes and accumulation of secondary metabolites.Abstract Rice (Oryza sativa L.), one of the most important staple foods worldwide, is frequently attacked by various herbivores, including brown planthopper (BPH, Nilaparvata lugens). BPH is a typical monophagous, phloem-sucking herbivore that has been a substantial threat to rice production and global food security. Understanding the regulatory mechanism of defense responses to BPH is essential for improving BPH resistance in rice. In this study, a SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE 10 (OsSPL10) transcription factor was found to play a negative role in the defenses of rice against BPH. To gain insights into the molecular and biochemical mechanisms of OsSPL10, we performed combined analyses of transcriptome and metabolome, and revealed that knockout of OsSPL10 gene improved rice resistance against BPH by enhancing the direct and indirect defenses. Genes involved in plant hormone signal transduction, MAPK signaling pathway, phenylpropanoid biosynthesis, and plant-pathogen interaction pathway were significantly upregulated in spl10 mutant. Moreover, spl10 mutant exhibited increased accumulation of defense-related secondary metabolites in the phenylpropanoid and terpenoid pathways. Our findings reveal a novel role for OsSPL10 gene in regulating the rice defense responses, which can be used as a potential target for genetic improvement of BPH resistance in rice.
Keyword ：
BPH resistance BPH resistance Plant defense response Plant defense response Rice Rice Secondary metabolites Secondary metabolites SPL gene SPL gene
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| GB/T 7714 | Lu, Long , Sun, Zhongxiang , Wang, Rumeng et al. Integration of transcriptome and metabolome analyses reveals the role of OsSPL10 in rice defense against brown planthopper [J]. | PLANT CELL REPORTS , 2023 , 42 (12) : 2023-2038 . |
| MLA | Lu, Long et al. "Integration of transcriptome and metabolome analyses reveals the role of OsSPL10 in rice defense against brown planthopper" . | PLANT CELL REPORTS 42 . 12 (2023) : 2023-2038 . |
| APA | Lu, Long , Sun, Zhongxiang , Wang, Rumeng , Du, Yifei , Zhang, Zaoli , Lan, Tao et al. Integration of transcriptome and metabolome analyses reveals the role of OsSPL10 in rice defense against brown planthopper . | PLANT CELL REPORTS , 2023 , 42 (12) , 2023-2038 . |
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Plant trichomes are a specialized cellular tissue that functions in resistance to biotic , abiotic stresses. In rice, three transcription-factor genes: OsWOX3B, HL6, OsSPL10, have been found to control tri-chome development. Although studies have shown interactions between the three genes, their full rela-tionship in trichome development is unclear. We found that the expression levels of OsWOX3B and HL6 were both reduced in OsSPL10-knockout plants but increased in OsSPL10-overexpression plants, suggest-ing that OsSPL10 positively regulates their expression. Physical interaction between OsSPL10 and OsWOX3B was found both in vivo and in vitro and attenuated their abilities to bind to the promoter of HL6 to activate its transcription. This mechanism may regulate trichome length by adjusting the expres-sion of HL6. A rice gene network regulating trichome development is proposed. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keyword ：
HL6 HL6 Interaction Interaction OsSPL10 OsSPL10 OsWOX3B OsWOX3B Rice Rice Trichome Trichome
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| GB/T 7714 | Liao, Qiaoling , Cheng, Xinle , Lan, Tong et al. OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.) [J]. | CROP JOURNAL , 2023 , 11 (6) : 1711-1718 . |
| MLA | Liao, Qiaoling et al. "OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.)" . | CROP JOURNAL 11 . 6 (2023) : 1711-1718 . |
| APA | Liao, Qiaoling , Cheng, Xinle , Lan, Tong , Guo, Xiaokuan , Su, Zilong , An, Xiaoxiao et al. OsSPL10 controls trichome development by interacting with OsWOX3B at both transcription and protein levels in rice (Oryza sativa L.) . | CROP JOURNAL , 2023 , 11 (6) , 1711-1718 . |
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Rf(fa), a new restoring fertility gene in rice, was previously located to a large region on Chromosome 10. The large number of genes within the region made cloning of Rf(fa) difficult. To perform the cloning and further elucidate the molecular mechanism, we reconstructed a mapping segregation population (BC1F1) of 12 000 plants. Using the population and polymorphism of simple sequence repeat (SSR) molecular markers, we finally mapped Rf(fa) between the two SSR molecular markers MM2000 and RM25658, within a 78.87 kb region. By de novo sequencing of a resto-ring line of CMS-FA hybrid rice, we obtained the genomic sequence of the mapping region, which provided the basis for the prediction of the candidate gene(s) of the target gene and for the comparison of genomic sequence differences between wild and cultivated rice. Within the mapping region, the genomic sequence of the wild rice was significantly different from that of cultivated rice. There were ten genes in the final mapping region. A pentatricopeptide repeat (PPR) protein gene was predicted as the candidate gene of Rf(fa). Our results laid a solid foundation for the final cloning and molecular mechanism analysis of the gene. The identified molecular markers tightly linked to Rf(fa) will facilitate the marker assisted selection in breeding of CMS-FA hybrid rice.
Keyword ：
fertility restorer fertility restorer gene fine mapping gene fine mapping prediction of candidate gene(s) prediction of candidate gene(s) rice rice
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| GB/T 7714 | Li, Yu , Chen, Xiaomi , Lan, Tao et al. Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice [J]. | CZECH JOURNAL OF GENETICS AND PLANT BREEDING , 2022 , 59 (1) : 43-53 . |
| MLA | Li, Yu et al. "Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice" . | CZECH JOURNAL OF GENETICS AND PLANT BREEDING 59 . 1 (2022) : 43-53 . |
| APA | Li, Yu , Chen, Xiaomi , Lan, Tao , Zhang, Jing , Chen, Ziheng , Yang, Wenting et al. Further fine mapping and candidate gene prediction for a new restoring fertility gene Rf(fa) in rice . | CZECH JOURNAL OF GENETICS AND PLANT BREEDING , 2022 , 59 (1) , 43-53 . |
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[目的]通过观察盐胁迫对水稻OsSPL10突变形成的苗期耐盐突变体sst种子萌发及幼苗生长的影响，探究突变体sst在芽期是否具有耐盐优势，以期为水稻耐盐育种提供依据。[方法]对籼稻品种R401的野生型和突变体sst进行盐胁迫下的发芽试验，统计发芽势和发芽率，并比较种子萌发后芽苗期的生长状况(根长、根数、地上部长和苗鲜重)。[结果]OsSPL10突变降低了非胁迫条件下水稻种子的发芽势和发芽率，增强了芽苗期水稻侧根对盐胁迫的敏感度。盐胁迫对野生型与突变体的各项指标均有抑制作用，且突变体sst在生长初期比野生型对盐胁迫更敏感，突变体耐盐性随着生长发育逐渐提高。[结论]水稻苗期耐盐突变体sst的苗期耐盐性与芽期没有相关性，在水稻耐盐性分子机理研究和耐盐育种实践中应加以区别。
Keyword ：
OsSPL10 OsSPL10 水稻 水稻 盐胁迫 盐胁迫 芽苗期 芽苗期 苗期耐盐突变体 苗期耐盐突变体
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| GB/T 7714 | 朱欢欢 , 廖巧玲 , 兰涛 . 盐胁迫对水稻耐盐突变体sst芽苗期生长的影响 [J]. | 亚热带农业研究 , 2022 , 18 (01) : 53-58 . |
| MLA | 朱欢欢 et al. "盐胁迫对水稻耐盐突变体sst芽苗期生长的影响" . | 亚热带农业研究 18 . 01 (2022) : 53-58 . |
| APA | 朱欢欢 , 廖巧玲 , 兰涛 . 盐胁迫对水稻耐盐突变体sst芽苗期生长的影响 . | 亚热带农业研究 , 2022 , 18 (01) , 53-58 . |
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【目的】水稻OsWOX3B基因调控叶片形态和表皮毛发育,根据表型被命名为LSY1、DEP、NUDA和GLR1等。深入了解OsWOX3B基因对水稻发育调控的功能具有重要意义。【方法】利用CRISPR/Cas9基因编辑技术对籼稻品种R401的OsWOX3B进行基因敲除。对所获材料进行突变位点分析和表型分析,同时进行相关基因的表达分析。【结果】所获材料的OsWOX3B基因的编码区第341位碱基由T变为C,第395-397位碱基缺失,其叶片和颖壳光滑,与突变体dep、nuda、glr1的表型相同,可确认其为Oswox3b突变体。除了与已报道的OsWOX3B的功能缺失突变表型相关外,Oswox3b突变体也有新表型出现。与野生型R401相比,突变体Oswox3b表现为生育期延长、分蘖数减少、叶片变宽、稻穗变长和每穗粒数增多。同时,突变体Oswox3b的剑叶维管束增多,小维管束间距增大,2个水稻侧生器官发育相关基因在突变体Oswox3b中的表达变化也与其表型变化一致。【结论】鉴定了一个新的水稻OsWOX3B基因突变体,其影响水稻侧生器官发育。
Keyword ：
侧生器官 侧生器官 水稻 水稻 生育期 生育期 维管束 维管束
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| GB/T 7714 | 郑亚莉 , 余林闯 , 安肖肖 et al. 一份水稻OsWOX3B基因敲除突变体的鉴定 [J]. | 中国水稻科学 , 2021 , 35 (02) : 112-120 . |
| MLA | 郑亚莉 et al. "一份水稻OsWOX3B基因敲除突变体的鉴定" . | 中国水稻科学 35 . 02 (2021) : 112-120 . |
| APA | 郑亚莉 , 余林闯 , 安肖肖 , 程心乐 , 任丽君 , 苏子龙 et al. 一份水稻OsWOX3B基因敲除突变体的鉴定 . | 中国水稻科学 , 2021 , 35 (02) , 112-120 . |
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