Query：
学者姓名：孙新立
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
Rice plants synthesize a unique group of diterpenoid phytoalexins (DPs) that exhibit broad-spectrum antimicrobial activities and are biosynthesized by enzymes encoded by three biosynthetic gene clusters. However, the regulatory mechanisms of their biosynthesis remain unclear. Here, the regulatory roles of the transcription factor OsWRKY10 and its interacting VQ motif-containing protein OsVQ8 in DPs biosynthesis and disease resistance were investigated via genetic and biochemical analyses. Their CRISPR/Cas9-mediated knockout and over-expressing (OE) lines, as well as crossed lines WRKY10OE/vq8, were generated. OsVQ8 phosphorylation by mitogen-activated protein kinase (MAPK) cascades was examined. We found that OsWRKY10 co-expresses with and activates a specific set of genes involved in DPs biosynthesis, thereby enhancing DPs accumulation and disease resistance against both fungal blast and bacterial blight. We demonstrate that OsWRKY10 interacts with the VQ motif-containing protein OsVQ8, modulating DPs biosynthesis through OsVQ8 phosphorylation by the activated OsMKK4-OsMPK6 cascade upon perception of pathogen-associated molecular patterns. Our findings highlight how the interaction between OsVQ8 and OsWRKY10 serves as a molecular switch to regulate gene clusters and the entire pathway of DPs biosynthesis in rice and provides valuable insights for genetic engineering aimed at enhancing phytoalexin production and broad-spectrum disease resistance in staple food crops.
Keyword ：
disease resistance disease resistance diterpenoid phytoalexins diterpenoid phytoalexins Oryza sativa L. Oryza sativa L. OsMKK4 OsMKK4 OsMPK6 OsMPK6 OsVQ8 OsVQ8 OsWRKY10 OsWRKY10
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Lin, Xianhui , Ding, Chaohui , Xiao, Wei et al. A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance [J]. | NEW PHYTOLOGIST , 2025 , 246 (5) : 2243-2262 . |
| MLA | Lin, Xianhui et al. "A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance" . | NEW PHYTOLOGIST 246 . 5 (2025) : 2243-2262 . |
| APA | Lin, Xianhui , Ding, Chaohui , Xiao, Wei , Wang, Jinhao , Lin, Zhuo , Sun, Xinli et al. A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance . | NEW PHYTOLOGIST , 2025 , 246 (5) , 2243-2262 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
植物F-box蛋白MAX2是独角金内酯(strigolactones, SLs)和烟素(karrinkins, KARs)信号传导的关键作用因子，作为SKP1-CUL1-FBX(SCF)复合体型泛素连接酶E3的主要成分，负责特异性识别并介导靶蛋白SMAX1/SMXLs的泛素化降解，从而调控植物形态建成和抗逆反应。重要粮油作物大豆(Glycine max)中亦存在MAX2的同源基因，然而其在植物防御反应中的功能却尚未被解析。为研究大豆中MAX2的同源基因的功能，本研究在生长受胁迫的大豆中成功克隆得到MAX2的同源基因GmMAX2b。生物信息学分析表明大豆存在2个MAX2同源基因GmMAX2a和GmMAX2b，相似度达96.2%,F-box区段高度保守。植物MAX2同源物蛋白序列比对及聚类分析基本反应了植物进化的亲缘关系，也提示大豆MAX2可能是多功能蛋白。表达分析表明，与拟南芥MAX2类似，植物病原菌侵染和水杨酸处理均能诱导大豆GmMAX2b表达。异位表达GmMAX2b能够回补max2-2突变体的感病性，说明GmMAX2b对植物抗病反应有正调节功能。此外，采用酵母双杂技术探寻GmMAX2b潜在的互作靶蛋白，发现其与SMXL6互作，与SMXL2存在微弱互作。这表明GmMAX2b是防御反应中正调控因子，受病原菌侵染和水杨酸处理诱导表达，其可能通过与SMXL6和SMXL2互作而发挥作用。本研究拓展了对大豆抗病F-box的理论认知，为今后大豆抗病育种提供了科学依据。
Keyword ：
GmMAX2b GmMAX2b 基因克隆 基因克隆 大豆 大豆 植物抗病反应 植物抗病反应
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 傅嘉辉 , 左林 , 黄伟群 et al. 大豆GmMAX2b基因克隆及抗病功能分析 [J]. | 生物工程学报 , 2025 , 41 (07) : 2803-2817 . |
| MLA | 傅嘉辉 et al. "大豆GmMAX2b基因克隆及抗病功能分析" . | 生物工程学报 41 . 07 (2025) : 2803-2817 . |
| APA | 傅嘉辉 , 左林 , 黄伟群 , 孙松 , 郭良雨 , 胡闽 et al. 大豆GmMAX2b基因克隆及抗病功能分析 . | 生物工程学报 , 2025 , 41 (07) , 2803-2817 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Transcription-factors (s) play key roles-in-the-regulatory-network-of-leaf senescence. However, many nodes in this network remain unclear. To elucidate the mechanism of leaf senescence mediated by a rice TF, WRKY10, the expression of multiple senescence-related genes and physiological phenotypes were monitored in WRKYO- and VQ MOTIF-CONTAINING PROTEINS (VQ8)-overexpressing plants and the wrky10 and vq8 mulants. Our results showed that WRKY10 positively regulates abscisic acid (ABA)-and dark-induced senescence (DIS) by directly regulating the expression of multiple senescence-related genes. The VOS protein, a repressor of WKKY10, negatively regulates WRKY10-mediated DIS. The WRKY10-VQ8 medule fine-tunes the progression of DIS. ABA, methyl jasmonate, and H2O, accelerate WRKY10-mediated DIS, whereas ammonium nitrate and dithiothreitol delay WRKY10-mediated DIS. Further analysis revealed that WRKY10 and VQ8 interact with ABA RESPONSIVE ELEMENT BINDING FACTOR1 (ABF1) or ABF2. VQ8 represses the transcriptional activity of ABF1 and ABF2. Overexpression of ABF1 or ABF2 accelerates ABA-and dark-induced senescence and H2O2 accumulation in N. benthamiana leaves, and WRKY10 and VQ8 can inhibit either ABF1- or ABF2-induced cell necrosis. Taken together, WRKY10 integrates hultiple senescence signals to establish an orderly progression of leaf senescence. The VQ8 protein actsths a brake on WRKY10-induced senescence and ABF1/2-induced cell death, prevent-ing uncontrolled cell death. 2025 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC- ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
Keyword ：
ABA ABA Darkness Darkness Leaf senescence Leaf senescence Oryza sativa Oryza sativa WRKY10-VQ8 module WRKY10-VQ8 module
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Sique , Yang, Xianfeng , Cao, Hongrui et al. WRKY10 and ABF1/2 bind to VQ8 to form an accelerator-brake module for the regulation of dark- and ABA-induced leaf senescence in rice [J]. | CROP JOURNAL , 2025 , 13 (1) : 145-157 . |
| MLA | Chen, Sique et al. "WRKY10 and ABF1/2 bind to VQ8 to form an accelerator-brake module for the regulation of dark- and ABA-induced leaf senescence in rice" . | CROP JOURNAL 13 . 1 (2025) : 145-157 . |
| APA | Chen, Sique , Yang, Xianfeng , Cao, Hongrui , Huang, Baolin , Zheng, Xiujuan , Xie, Wenjia et al. WRKY10 and ABF1/2 bind to VQ8 to form an accelerator-brake module for the regulation of dark- and ABA-induced leaf senescence in rice . | CROP JOURNAL , 2025 , 13 (1) , 145-157 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Arabidopsis MORE AXILLARY GROWTH2 (MAX2) is a key component in the strigolactone (SL) and karrikin (KAR) signaling pathways and regulates the degradation of SUPPRESSOR OF MAX2 1/SMAX1-like (SMAX1/SMXL) proteins, which are transcriptional co-repressors that regulate plant architecture, as well as abiotic and biotic stress responses. The max2 mutation reduces resistance against Pseudomonas syringae pv. tomato (Pst). To uncover the mechanism of MAX2-mediated resistance, we evaluated the resistance of various SL and KAR signaling pathway mutants. The resistance of SL-deficient mutants and of dwarf 14 (d14) was similar to that of the wild-type, whereas the resistance of the karrikin insensitive 2 (kai2) mutant was compromised, demonstrating that the KAR signaling pathway, not the SL signaling pathway, positively regulates the immune response. We measured the resistance of smax1 and smxl mutants, as well as the double, triple, and quadruple mutants with max2, which revealed that both the smax1 mutant and smxl6/7/8 triple mutant rescue the low resistance phenotype of max2 and that SMAX1 accumulation diminishes resistance. The susceptibility of smax1D, containing a degradation-insensitive form of SMAX1, further confirmed the SMAX1 function in the resistance. The relationship between the accumulation of SMAX1/SMXLs and disease resistance suggested that the inhibitory activity of SMAX1 to resistance requires SMXL6/7/8. Moreover, the exogenous application of KAR2 enhanced resistance against Pst, but KAR-induced resistance depended on salicylic acid (SA) signaling. Inhibition of karrikin signaling delayed SA-mediated defense responses and inhibited pathogen-induced protein biosynthesis. Together, we propose that the MAX2-KAI2-SMAX1 complex regulates resistance with the assistance of SMXL6/7/8 and SA signaling and that SMAX1/SMXLs possibly form a multimeric complex with their target transcription factors to fine tune immune responses.
Keyword ：
degradation degradation immunity immunity karrikin karrikin pathway pathway SMAX1 SMAX1 SMXLs SMXLs strigolactone strigolactone transcription factor transcription factor
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zheng, Xiujuan , Liu, Fangqian , Yang, Xianfeng et al. The MAX2-KAI2 module promotes salicylic acid-mediated immune responses in Arabidopsis [J]. | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2023 , 65 (6) : 1566-1584 . |
| MLA | Zheng, Xiujuan et al. "The MAX2-KAI2 module promotes salicylic acid-mediated immune responses in Arabidopsis" . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY 65 . 6 (2023) : 1566-1584 . |
| APA | Zheng, Xiujuan , Liu, Fangqian , Yang, Xianfeng , Li, Weiqiang , Chen, Sique , Yue, Xinwu et al. The MAX2-KAI2 module promotes salicylic acid-mediated immune responses in Arabidopsis . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2023 , 65 (6) , 1566-1584 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
WRKY transcription factors (TFs) play crucial roles in biotic and abiotic stress responses. However, their roles in thermal response are still largely elusive, especially in rice. In this study, we revealed the functions of WRKY10 TF and VQ8 protein containing VQ motif in rice thermotolerance. Overexpression of WRKY10 or loss of VQ8 function increases thermosensitivity, whereas conversely, overexpression of VQ8 or loss of WRKY10 function enhances thermotolerance. Overexpression of WRKY10 accelerates reactive oxygen species (ROS) accumulation in chloroplasts and apoplasts, and it also induces the expression of heat shock TF and protein genes. We also found that WRKY10 regulates nuclear DNA fragmentation and hypersensitive response by modulating NAC4 TF expression. The balance between destructive and protective responses in WRKY10-overexpression plant is more fragile and more easily broken by heat stress compared with wild type. In vitro and in vivo assays revealed that VQ8 interacts with WRKY10 and inhibits the transcription activity via repressing its DNA-binding activity. Our study demonstrates that WRKY10 negatively regulates thermotolerance by modulating the ROS balance and the hypersensitive response and that VQ8 functions antagonistically to positively regulate thermotolerance. The functional module of WRKY10-VQ8 provides safe and effective regulatory mechanisms in the heat stress response.
Keyword ：
chloroplast chloroplast heat stress heat stress interaction interaction mutant mutant NAC4 NAC4 overexpression overexpression ROS ROS
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Chen, Sique , Cao, Hongrui , Huang, Baolin et al. The WRKY10-VQ8 module safely and effectively regulates rice thermotolerance [J]. | PLANT CELL AND ENVIRONMENT , 2022 , 45 (7) : 2126-2144 . |
| MLA | Chen, Sique et al. "The WRKY10-VQ8 module safely and effectively regulates rice thermotolerance" . | PLANT CELL AND ENVIRONMENT 45 . 7 (2022) : 2126-2144 . |
| APA | Chen, Sique , Cao, Hongrui , Huang, Baolin , Zheng, Xiujuan , Liang, Kangjing , Wang, Guo-Liang et al. The WRKY10-VQ8 module safely and effectively regulates rice thermotolerance . | PLANT CELL AND ENVIRONMENT , 2022 , 45 (7) , 2126-2144 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Arabidopsis Casparian strip membrane domain proteins (CASPs) form a transmembrane scaffold to recruit lignin biosynthetic enzymes for Casparian strip (CS) formation. Rice is a semi-aquatic plant with a more complex root structure than Arabidopsis to adapt its growing conditions, where the different deposition of lignin and suberin is crucial for adaptive responses. Here, we observed the structure of rice primary and small lateral roots (SLRs), particularly the deposition patterns of lignin and suberin in wild type and Oscasp1 mutants. We found that the appearance time and structure of CS in the roots of rice are different from those of Arabidopsis and observed suberin deposition in the sclerenchyma in wild type roots. Rice CASP1 is highly similar to AtCASPs, but its expression is concentrated in SLR tips and can be induced by salt stress especially in the steles. The loss of OsCASP1 function alters the expression of the genes involved in suberin biosynthesis and the deposition of suberin in the endodermis and sclerenchyma and leads to delayed CS formation and uneven lignin deposition in SLRs. These different depositions may alter nutrient uptake, resulting in ion imbalance in plant, withered leaves, fewer tillers, and reduced tolerance to salt stress. Our findings suggest that OsCASP1 could play an important role in nutrient homeostasis and adaptation to the growth environment.
Keyword ：
CASP CASP endodermis endodermis exodermis exodermis ion ion leaf senescence leaf senescence salt stress salt stress sclerenchyma sclerenchyma
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Xianfeng , Xie, Huifang , Weng, Qunqing et al. Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis [J]. | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
| MLA | Yang, Xianfeng et al. "Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis" . | FRONTIERS IN PLANT SCIENCE 13 (2022) . |
| APA | Yang, Xianfeng , Xie, Huifang , Weng, Qunqing , Liang, Kangjing , Zheng, Xiujuan , Guo, Yuchun et al. Rice OsCASP1 orchestrates Casparian strip formation and suberin deposition in small lateral roots to maintain nutrient homeostasis . | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Arabidopsis: SMAX1/SMXL (SUPPRESSOR OF MAX2 1/SMAX1-LIKE) proteins function as transcriptional repressors in karrikin and strigolactone (SL) signaling pathways and regulate plant architecture. MAX2 is a common factor in the two signaling pathways and a component of the SCF complex that modulates the proteasome-mediated degradation of SMAX1/SMXLs. SMXL6, 7, and 8 proteins promote shoot branching and inhibit petiole elongation. Our study found that the accumulation of SMAX1 suppresses rosette shoot branching and increases cauline branches on the primary inflorescence stem, plant height, petiole length, and leaf length/width ratio. The SMAX1 accumulation enhances the expression of BRC1, HB53, HB40, and HB21 that modulate shoot branching. SMAX1 also regulates the expression of the genes involved in auxin transport, cytokinin signaling pathway, and SL biosynthesis. The expression analyses of these genes suggest that excessive SMAX1 should accelerate the transport of auxin and the biosynthesis of SL in plants. High SL concentration suppresses the bud development in smax1D mutant that accumulates SMAX1 protein in plant. However, the effects of cytokinin and auxin on shoot branching remain elusive in the mutant with excessive SMAX1. SMAX1 regulates leaf shape and petiole length via modulating TCP1 expression. Our findings reveal a novel function of SMAX1 and new mechanism of shoot branching.
Keyword ：
Branches Branches Leaf shape Leaf shape SMAX1 SMAX1
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zheng, Xiujuan , Yang, Xianfeng , Chen, Zheng et al. Arabidopsis SMAX1 overaccumulation suppresses rosette shoot branching and promotes leaf and petiole elongation [J]. | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 553 : 44-50 . |
| MLA | Zheng, Xiujuan et al. "Arabidopsis SMAX1 overaccumulation suppresses rosette shoot branching and promotes leaf and petiole elongation" . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 553 (2021) : 44-50 . |
| APA | Zheng, Xiujuan , Yang, Xianfeng , Chen, Zheng , Xie, Wenjia , Yue, Xinwu , Zhu, Haipeng et al. Arabidopsis SMAX1 overaccumulation suppresses rosette shoot branching and promotes leaf and petiole elongation . | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS , 2021 , 553 , 44-50 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
植物生物技术的应用为人类创造了巨大的经济效益,鉴于此,很多农业院校多年前就已经开设了植物生物技术课程。作者在这几年的教学实践中,深刻地感受到一些教学中的问题严重影响了学生的学习积极性和学习效果。本文通过对这些问题的分析,提出了相应的改进措施。
Keyword ：
学习积极性 学习积极性 实验 实验 植物生物技术 植物生物技术
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 孙新立 . 农科专业植物生物技术教学中的问题与思考 [J]. | 教育教学论坛 , 2018 , (04) : 218-219 . |
| MLA | 孙新立 . "农科专业植物生物技术教学中的问题与思考" . | 教育教学论坛 04 (2018) : 218-219 . |
| APA | 孙新立 . 农科专业植物生物技术教学中的问题与思考 . | 教育教学论坛 , 2018 , (04) , 218-219 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
LPBG08是本研究室培育的一个特大粒水稻种质,其平均粒长达15 mm,平均粒宽达4.2 mm,平均千粒重达66 g。为了解析该特异种质粒形性状形成的遗传机理,本研究以其为供试材料,利用目的基因扩增和测序手段,对2个重要粒长基因(GS3,qGL3/GL3.1)和4个重要粒宽基因(GW2,GW8,GS5和GW5)进行检测。检测发现,6个粒形相关基因中有5个在LPBG08中发生了正调控突变。其中,GS3和qGL3/GL3.1 2个粒长基因全都发生突变,4个控制粒宽基因中的GW2、GW8和GW5等3个发生突变。研究结果表明,LPBG08的特大粒粒形性状的形成可能是由于多个正向调控突变的粒形相关基因聚合所引起的累加效应。
Keyword ：
水稻 水稻 粒宽 粒宽 粒形 粒形 粒长 粒长
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 陈杰 , 朱业宝 , 孙新立 et al. 一个特大粒水稻种质粒形相关基因的检测 [J]. | 分子植物育种 , 2017 , 15 (02) : 576-581 . |
| MLA | 陈杰 et al. "一个特大粒水稻种质粒形相关基因的检测" . | 分子植物育种 15 . 02 (2017) : 576-581 . |
| APA | 陈杰 , 朱业宝 , 孙新立 , 翁群清 , 张国杰 , 梁康迳 . 一个特大粒水稻种质粒形相关基因的检测 . | 分子植物育种 , 2017 , 15 (02) , 576-581 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
ASK1-D3-D14复合体作为E3泛素连接酶的一部分,调控水稻的形态发生等生理反应.为了揭示该复合体互作区的结构特征和演化关系,通过对不同物种中D3直系蛋白间的系统进化进行分析,发现D3基因在不同物种中表现出不同的演化进程;而对ASK1-D3和D3-D14互作面的序列和空间结构分析,揭示D3直系同源蛋白质以及已知的F-box-like结构域间的序列同源性较低,而空间结构相似性较高,暗示F-box-like与ASK1类蛋白质之间的互作,空间结构的相似性重于序列的相似性.涉及D3和D14互作面的氨基酸在直系同源蛋白序列间的同源性很高,而形成互作面疏水内核的氨基酸保守性更高.这为D3复合体的进一步研究提供了依据.
Keyword ：
ASK1-D3-D14 ASK1-D3-D14 互作 互作 演化 演化 蛋白复合体 蛋白复合体
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 郑秀娟 , 翁群清 , 陈思雀 et al. 植物ASK1-D3(MAX2)-D14蛋白复合体互作区特征与演化分析 [J]. | 福建农林大学学报(自然科学版) , 2017 , 46 (05) : 539-545 . |
| MLA | 郑秀娟 et al. "植物ASK1-D3(MAX2)-D14蛋白复合体互作区特征与演化分析" . | 福建农林大学学报(自然科学版) 46 . 05 (2017) : 539-545 . |
| APA | 郑秀娟 , 翁群清 , 陈思雀 , 孙新立 . 植物ASK1-D3(MAX2)-D14蛋白复合体互作区特征与演化分析 . | 福建农林大学学报(自然科学版) , 2017 , 46 (05) , 539-545 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
