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学者姓名：张重义
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Abstract ：
本发明涉及药用植物栽培技术领域，尤其涉及一种协同改良金线莲抗性、产量和品质的LED光配方及其应用；本发明利用LED蓝光、红光、远红光组配对移栽后金线莲进行处理，处理组植株的抗氧化酶活性和木质素含量较对照组显著提高，茎腐病发病进程延缓、发病率和病情指数明显下降；本发明提出的LED光配方在金线莲生产中具有稳产效应，且有助于增加金线莲干品产量；本发明提出的LED光配方有助于金线莲中有效活性成分多糖的积累，可促进金线莲多糖产品的深度开发。本发明可实现金线莲抗病性、生物量积累和多糖含量的协同改良。
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| GB/T 7714 | 郭晓磊 , 古力 , 李明杰 et al. 协同改良金线莲抗性、产量和品质的LED光配方及其应用 : CN202411531281.3[P]. | 2024-10-30 . |
| MLA | 郭晓磊 et al. "协同改良金线莲抗性、产量和品质的LED光配方及其应用" : CN202411531281.3. | 2024-10-30 . |
| APA | 郭晓磊 , 古力 , 李明杰 , 王建明 , 刘铁东 , 王衍坤 et al. 协同改良金线莲抗性、产量和品质的LED光配方及其应用 : CN202411531281.3. | 2024-10-30 . |
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本发明提供了一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法，属于药材鉴别技术领域。本发明包括以下步骤：采集不同太子参的高光谱图像数据；对采集的太子参高光谱图像数据进行黑白板校正，计算校正高光谱数据的相对反射率；对所述相对反射率数据进行阈值分割，提取目标感兴趣区域，计算感兴趣区域平均光谱值；剔除感兴趣区域平均光谱值的异常数据，获得原始全波段光谱数据；对原始全波段光谱数据进行特征波段筛选，将筛选获得的特征波段与种质信息利用粒子优化算法构建太子参种质鉴别模型。本发明利用高光谱成像技术构建的模型可用于太子参的分类鉴别，分辨率和灵敏度高，重复性好，简便快捷，具有广泛的应用价值。
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| GB/T 7714 | 张婷婷 , 童金鹏 , 古力 et al. 一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法 : CN202510280748.X[P]. | 2025-03-11 . |
| MLA | 张婷婷 et al. "一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法" : CN202510280748.X. | 2025-03-11 . |
| APA | 张婷婷 , 童金鹏 , 古力 , 李明杰 , 张重义 , 王衍坤 et al. 一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法 : CN202510280748.X. | 2025-03-11 . |
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The timing of transplanting and topping significantly affects transcriptional expression, thereby influencing the metabolic and developmental processes of tobacco. The glycolysis pathway plays a central role in driving internal chemical changes that shape alterations in the tobacco's metabolic pathways. Transcriptome analysis revealed that early transplanting and topping treatments resulted in 4,534 differentially expressed genes. KEGG annotation identified 32 differentially expressed genes associated with glycolysis metabolism. Specifically, the aldehyde dehydrogenase (NAD(+)) (EC 1.2.1.3) pathway was significantly upregulated, whereas the fructose-bisphosphate aldolase (EC 4.1.2.13) was significantly downregulated. KEGG-GSEA gene set analysis indicated that early transplanting and topping enhanced expression of gene sets involved in glycolysis metabolism. Additionally, two core enrichment genes, KPYC-l and Gal-l, were identified as being involved in glycolysis metabolism. Across the glycolysis metabolic pathway, enzyme activities were predominantly downregulated before phosphoenolpyruvate and upregulated thereafter. This suggests that tobacco likely enhanced phosphoenolpyruvate production through increased activity in the pentose phosphate pathway and gluconeogenesis. These alterations promoted heightened metabolic pathways involving pyruvate, acetate, and acetyl-CoA. A newly identified gene, LOC107803480, belonging to the SCF family, was found to be enriched in glycolysis metabolism according to the KEGG database. This gene contains three conserved motifs: PS00184 (GARS), PS00337 (BETA_LACTAMASE_D), and PS10049 (PSI_RSU). In conclusion, transcriptional analysis and the discovery of novel genes suggest that early transplanting and topping significantly impacts the glycolysis pathway, underscoring its importance in carbohydrate metabolism in tobacco. (c) 2025 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
Keyword ：
Glycolysis Glycolysis Tobacco Tobacco Topping Topping Transcriptome Transcriptome Transplanting Transplanting
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| GB/T 7714 | Zhao, Xianbo , Yuan, Feiyue , Chen, Yiqiang et al. Effects of early transplanting and topping on glycolysis in the upper leaves of Nicotiana tabacum [J]. | SOUTH AFRICAN JOURNAL OF BOTANY , 2025 , 178 : 217-225 . |
| MLA | Zhao, Xianbo et al. "Effects of early transplanting and topping on glycolysis in the upper leaves of Nicotiana tabacum" . | SOUTH AFRICAN JOURNAL OF BOTANY 178 (2025) : 217-225 . |
| APA | Zhao, Xianbo , Yuan, Feiyue , Chen, Yiqiang , Zhang, Yiqi , Zhang, Ye , Zhang, Zhuangyi et al. Effects of early transplanting and topping on glycolysis in the upper leaves of Nicotiana tabacum . | SOUTH AFRICAN JOURNAL OF BOTANY , 2025 , 178 , 217-225 . |
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The Agrobacterium tumefaciens mediated transformation is the prevailing methodology for plant genetic manipulation; however, A. tumefaciens overgrowth is a common constraint in the process. Exploring auxotrophic A. tumefaciens could reduce overgrowth and enhance plant transformation efficiency. The ILVC gene, which encodes the ketol-acid isomeroreductase, is critical for Valine (Val) and isoleucine (Ile) biosynthesis in some microorganisms. However, its function in A. tumefaciens is unclear. To ascertain the function of ILVC and generate an auxotrophic A. tumefaciens, this study employed an allelic exchange to disrupt the ILVC in A. tumefaciens strain GV3101. This resulted in the loss of ketol-acid isomeroreductase activity and the prevention of Val and Ile biosynthesis, creating a dual-auxotrophic GV3101 triangle ILVC. Transient expression assays in Nicotiana benthamiana transformation demonstrated that the GV3101 triangle ILVC was capable of T-DNA transfer. Moreover, stable genetic transformation analysis in N. benthamiana indicated that the introduction of GV3101 triangle ILVC led to a reduction in overgrowth within infected plant tissues. Additionally, an enhancement in transformation efficiency was observed with the prolongation of the co-cultivation time of the explant-infected strain. This study revealed the function of ILVC and explored a dual-auxotrophic A. tumefaciens for Val and Ile, potentially broadening the utilization of auxotrophic strains in plant genetic transformation.
Keyword ：
Agrobacterium tumefaciens Agrobacterium tumefaciens Auxotrophy Auxotrophy ILVC gene ILVC gene Plant transformation Plant transformation Valine and isoleucine biosynthesis Valine and isoleucine biosynthesis
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| GB/T 7714 | Yang, Yanhui , Lu, Jun , Xue, Tianrui et al. Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation [J]. | TRANSGENIC RESEARCH , 2025 , 34 (1) . |
| MLA | Yang, Yanhui et al. "Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation" . | TRANSGENIC RESEARCH 34 . 1 (2025) . |
| APA | Yang, Yanhui , Lu, Jun , Xue, Tianrui , Cao, Zhenli , Li, Mingjie , Zhang, Zhongyi et al. Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation . | TRANSGENIC RESEARCH , 2025 , 34 (1) . |
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Anoectochilus roxburghii (Wall.) Lindl. (A. roxburghii) is an increasingly popular medicinal herb. Arbuscular mycorrhiza (AM) fungi, known for their symbiotic relationships with plant roots, enhance nutrient uptake and disease resistance in host plants. However, their specific regulatory mechanisms in A. roxburghii are not fully understood. In this study, Fujian A. roxburghii was inoculated with the AM fungus Glomus intraradices, and successful root colonization was observed. Following AM fungal colonization, there was a significant upregulation of photosynthesis-related genes in the stems, accompanied by improved canopy phenotypes and root architecture. Consequently, AM-inoculated plants exhibited increased fresh and dry biomass, as well as elevated levels of polysaccharides and flavonoids. Additionally, the incidence of Fusarium oxysporum-induced stalk rot was reduced in AM-inoculated plants. Analysis of defense-related enzymes indicated that AM-inoculated plants exhibited a rapid and robust response to pathogen infection, mitigating oxidative stress. Transcriptomic analysis revealed significant upregulation of genes associated "Fatty acid degradation", "MAPK signaling pathway-plant", and "Plant-pathogen interaction", suggesting their involvement in enhanced disease resistance. A regulatory network centered on ACX1 and calmodulin, involving multiple transcription factors such as WRKY, bHLH, ERF, NAC, and HSF, was implicated in defense responses. These findings demonstrated the beneficial effects of AM fungi on yield, quality, and disease resistance in A. roxburghii, providing a theoretical foundation for its cultivation and genetic improvement.
Keyword ：
Anoectochilus roxburghii Anoectochilus roxburghii Arbuscular mycorrhiza fungus Arbuscular mycorrhiza fungus Bioactive compound Bioactive compound Biomass accumulation Biomass accumulation Disease resistance Disease resistance
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| GB/T 7714 | Gu, Li , Li, Shurong , Zhou, Lichun et al. Ecophysiological and transcriptional landscapes of arbuscular mycorrhiza fungi enhancing yield, quality, and stalk rot resistance in Anoectochilus roxburghii [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 . |
| MLA | Gu, Li et al. "Ecophysiological and transcriptional landscapes of arbuscular mycorrhiza fungi enhancing yield, quality, and stalk rot resistance in Anoectochilus roxburghii" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 223 (2025) . |
| APA | Gu, Li , Li, Shurong , Zhou, Lichun , Yuan, Feiyue , Zhang, Tingting , Wang, Yankun et al. Ecophysiological and transcriptional landscapes of arbuscular mycorrhiza fungi enhancing yield, quality, and stalk rot resistance in Anoectochilus roxburghii . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 . |
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为探究枯草芽孢杆菌与生物炭配施对烟稻轮作区土壤微生物及烟叶质量的影响，为南方烟区烟稻轮作土壤保育、微生物群落调控及提高烟叶质量提供理论依据，采用大田试验设计，设不施肥（CK1）、常规施肥（CK2）、常规施肥+900 kg/hm
Keyword ：
土壤微生物 土壤微生物 枯草芽孢杆菌 枯草芽孢杆菌 烟叶质量 烟叶质量 烤烟 烤烟 生物炭 生物炭
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| GB/T 7714 | 闫伸 , 王鹏 , 陈承亮 et al. 生物炭配施芽孢杆菌对植烟土壤微生物群落结构及烟叶质量的影响 [J]. | 江苏农业科学 , 2025 , 53 (03) : 222-228 . |
| MLA | 闫伸 et al. "生物炭配施芽孢杆菌对植烟土壤微生物群落结构及烟叶质量的影响" . | 江苏农业科学 53 . 03 (2025) : 222-228 . |
| APA | 闫伸 , 王鹏 , 陈承亮 , 廖珠珠 , 汪立刚 , 王飞 et al. 生物炭配施芽孢杆菌对植烟土壤微生物群落结构及烟叶质量的影响 . | 江苏农业科学 , 2025 , 53 (03) , 222-228 . |
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Flavonoids are a major component of Artemisia argyi and play a crucial role in its pharmacological properties. However, the molecular mechanisms underlying flavonoid biosynthesis in A. argyi remain unclear. To address this, transcriptome and quantitative metabolome analyses were conducted across five developmental stages of A. argyi. In total, 85 flavonoid compounds were identified across these different stages. Differentially expressed candidate genes and metabolites involved in flavonoid biosynthesis were also identified. Differentially accumulated flavonoid metabolites (DFMs) were observed among the 10 comparison groups, with 29 DFMs identified from the five developmental stages of A. argyi leaves. The biosynthesis process identified 38 differentially expressed genes (DEGs) from seven gene families. Furthermore, 26 DEGs exhibited a significant correlation with the levels of seven active flavonoid metabolites, as revealed by weighted gene co-expression network analysis. These DEGs included eight HCT genes, six CHI genes, two CHS genes, three CCoAOMT genes, two F3 ' H genes, two C4H genes, two CYP98A genes, and one F3H gene. Based on preliminary analysis, HCT1 may be associated with accumulating hispidulin and jaceosidin. This study investigated the relationship between differential gene expression and flavonoid accumulation using an integrated transcriptomic and metabolomic approach, providing valuable insights into the mechanisms of flavonoid biosynthesis and quality formation in A. argyi.
Keyword ：
Artemisia argyi Artemisia argyi enzyme genes enzyme genes flavonoids flavonoids metabolome metabolome transcriptome transcriptome
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| GB/T 7714 | Miao, Chunyan , Li, Mengzhi , Li, Mingjie et al. Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages [J]. | PHYSIOLOGIA PLANTARUM , 2025 , 177 (4) . |
| MLA | Miao, Chunyan et al. "Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages" . | PHYSIOLOGIA PLANTARUM 177 . 4 (2025) . |
| APA | Miao, Chunyan , Li, Mengzhi , Li, Mingjie , Gu, Li , Zhang, Zhongyi , Gao, Li et al. Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages . | PHYSIOLOGIA PLANTARUM , 2025 , 177 (4) . |
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The homeodomain-leucine zipper (HD-Zip) family of transcription factor is a unique and vital gene family in plants, involved in various processes including organ development, stress responses, and regulation of secondary metabolite biosynthesis. This study aimed to investigate the role of HD-Zip family transcription factors in the development of Artemisia argyi trichomes. By utilizing the whole genome data of A. argyi, we identified 69 AaHDZ transcription factors and systematically characterized their structural and physicochemical properties. The predicted amino acid sequence lengths range from 188 to 854 residues, with relative molecular masses of 22.2-94.6 kDa, theoretical isoelectric points of 4.65-9.31, and lipid solubility indices of 52.08-89.14. All of these proteins were hydrophilic. The results of subcellular localization predictions indicated that most AaHDZ proteins are localized in the nucleus, while a few are located in the cytoplasm and chloroplasts. Based on the classification of the HD-Zip family in Arabidopsis thaliana, the 69 AaHDZ proteins were divided into four subfamilies, with similar gene structures observed within each subfamily. Heterologous overexpression of AaHDZ64 gene significantly increased trichome density in A. thaliana. The results confirmed that AaHDZ64 is a positive regulatory factor for the growth and development of trichomes. This study provides a theoretical foundation for understanding the molecular regulatory mechanisms underlying trichome development in A. argyi leaves and offers insights for the precision breeding of A. argyi.
Keyword ：
AaHDZ64 AaHDZ64 Artemisia argyi Artemisia argyi Expression analysis Expression analysis HD-Zip transcription factor HD-Zip transcription factor Trichome development Trichome development
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| GB/T 7714 | Cui, Zhanhu , Miao, Chunyan , Shang, Heyang et al. Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development [J]. | PLANT GENE , 2025 , 43 . |
| MLA | Cui, Zhanhu et al. "Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development" . | PLANT GENE 43 (2025) . |
| APA | Cui, Zhanhu , Miao, Chunyan , Shang, Heyang , Zhao, Qian , Li, Shujiao , Zhang, Zhongyi et al. Genome-wide identification of HD-Zip transcription factor family in Artemisia argyi and functional analysis of AaHDZ64 in trichome development . | PLANT GENE , 2025 , 43 . |
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Rehmannia glutinosa is an important medicinal herb; but its long-term cultivation often leads to continuous cropping problems. The underlying cause can be attributed to the accumulation of and alterations in root exudates; which interact with soil-borne pathogens; particularly Fusarium oxysporum; triggering disease outbreaks that severely affect its yield and quality. It is therefore crucial to elucidate the mechanisms by which root exudates induce F. oxysporum CCS043 outbreaks. In this study; the genome of F. oxysporum CCS043 from R. glutinosa's rhizosphere microbiota was sequenced and assembled de novo; resulting in a 47.67 Mb genome comprising 16,423 protein-coding genes. Evolutionary analysis suggests that different F. oxysporum strains may adapt to the host rhizosphere microecosystem by acquiring varying numbers of specific genes while maintaining a constant number of core genes.The allelopathic effects of ferulic acid; verbascoside; and catalpol on F. oxysporum CCS043 were examined at the physiological and transcriptomic levels. The application of ferulic acid was observed to primarily facilitate the proliferation and growth of F. oxysporum CCS043; whereas verbascoside notably enhanced the biosynthesis of infection-related enzymes such as pectinase and cellulase. Catalpol demonstrated a moderate level of allelopathic effects in comparison to the other two. Furthermore; 10 effectors were identified by combining the genomic data. Meanwhile; it was found that among the effector-protein-coding genes; ChiC; VRDA; csn; and chitinase exhibited upregulated expression across all treatments. The expression patterns of these key genes were validated using qRT-PCR. Transient overexpression of the two effector-encoding genes in detached R. glutinosa leaves provided further confirmation that ChiC (GME8876_g) and csn (GME9251_g) are key effector proteins responsible for the induction of hypersensitive reactions in R. glutinosa leaf cells. This study provides a preliminary indication that the use of allelochemicals by F. oxysporum CCS043 can promote its own growth and proliferation and enhance infection activity. This finding offers a solid theoretical basis and data support for elucidating the fundamental causes of fungal disease outbreaks in continuous cropping of R. glutinosa and for formulating effective mitigation strategies.
Keyword ：
allelopathy allelopathy effector protein effector protein F. oxysporum CCS043 F. oxysporum CCS043 Rehmannia glutinosa Rehmannia glutinosa root exudates root exudates
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| GB/T 7714 | Yuan, Feiyue , Qiu, Fuxiang , Xie, Jiawei et al. Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa [J]. | PLANTS-BASEL , 2025 , 14 (1) . |
| MLA | Yuan, Feiyue et al. "Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa" . | PLANTS-BASEL 14 . 1 (2025) . |
| APA | Yuan, Feiyue , Qiu, Fuxiang , Xie, Jiawei , Fan, Yongxi , Zhang, Bao , Zhang, Tingting et al. Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa . | PLANTS-BASEL , 2025 , 14 (1) . |
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The obstacle of continuous cropping poses a key challenge limiting the sustainable and safe production of Chinese herbal medicines, with the dysregulation of plant immune responses being identified as the primary contributing factor. LRR-RLPs, as major members of plant pattern recognition receptors (PRRs), are extensively involved in mediating plant immune responses. However, these receptors remain unidentified in the medicinal plant Rehmannia glutinosa. In this study, we identified 14 RgRLP genes in R. glutinosa through bioinformatics approaches and systematically analyzed their differential expression patternas under potted cultivation versus controlled simulated continuous cropping conditions. Subsequently, we constructed overexpression systems for functional characterization of three pivotal RgRLP genes (RgRLP4, RgRLP10, and RgRLP12), which were identified as central regulators of continuous cropping stress responses. Notably, continuous cropping stress induced severe oxidative damage in root tip tissues and promoted rhizosphere proliferation of Fusarium oxysporum via exudate-mediated interactions, thereby exacerbating stress effects. Importantly, overexpression of RgRLP genes significantly alleviated continuous cropping-induced stress damage. Furthermore, transcriptomic analysis of transgenic lines revealed marked upregulation of three LRR-RLK genes (RgRLK19, RgRLK27, and RgRLK30), suggesting their potential roles in RgRLP-mediated stress signaling pathways. This study provides a good theoretical basis and technical support for in-depth study of the formation mechanism and reduction strategies of continuous cropping obstacles of R. glutinosa.
Keyword ：
Continuous cropping obstacles Continuous cropping obstacles F. oxysporum F. oxysporum LRR-RLP genes LRR-RLP genes Rehmannia glutinosa Rehmannia glutinosa
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| GB/T 7714 | Qiu, Fuxiang , Yu, Zhijian , Fan, Yongxi et al. Screening and functional characterization of Rehmannia glutinosa receptor proteins (RgRLPs) in response to continuous cropping stress [J]. | SCIENTIA HORTICULTURAE , 2025 , 344 . |
| MLA | Qiu, Fuxiang et al. "Screening and functional characterization of Rehmannia glutinosa receptor proteins (RgRLPs) in response to continuous cropping stress" . | SCIENTIA HORTICULTURAE 344 (2025) . |
| APA | Qiu, Fuxiang , Yu, Zhijian , Fan, Yongxi , Zhang, Bao , Zhang, Guojun , Lai, Yanlin et al. Screening and functional characterization of Rehmannia glutinosa receptor proteins (RgRLPs) in response to continuous cropping stress . | SCIENTIA HORTICULTURAE , 2025 , 344 . |
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