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学者姓名：李明杰
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Abstract ：
Flavonoids are a major component of Artemisia argyi and play a crucial role in its pharmacological properties. However, the molecular mechanisms underlying flavonoid biosynthesis in A. argyi remain unclear. To address this, transcriptome and quantitative metabolome analyses were conducted across five developmental stages of A. argyi. In total, 85 flavonoid compounds were identified across these different stages. Differentially expressed candidate genes and metabolites involved in flavonoid biosynthesis were also identified. Differentially accumulated flavonoid metabolites (DFMs) were observed among the 10 comparison groups, with 29 DFMs identified from the five developmental stages of A. argyi leaves. The biosynthesis process identified 38 differentially expressed genes (DEGs) from seven gene families. Furthermore, 26 DEGs exhibited a significant correlation with the levels of seven active flavonoid metabolites, as revealed by weighted gene co-expression network analysis. These DEGs included eight HCT genes, six CHI genes, two CHS genes, three CCoAOMT genes, two F3 ' H genes, two C4H genes, two CYP98A genes, and one F3H gene. Based on preliminary analysis, HCT1 may be associated with accumulating hispidulin and jaceosidin. This study investigated the relationship between differential gene expression and flavonoid accumulation using an integrated transcriptomic and metabolomic approach, providing valuable insights into the mechanisms of flavonoid biosynthesis and quality formation in A. argyi.
Keyword ：
Artemisia argyi Artemisia argyi enzyme genes enzyme genes flavonoids flavonoids metabolome metabolome transcriptome transcriptome
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| GB/T 7714 | Miao, Chunyan , Li, Mengzhi , Li, Mingjie et al. Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages [J]. | PHYSIOLOGIA PLANTARUM , 2025 , 177 (4) . |
| MLA | Miao, Chunyan et al. "Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages" . | PHYSIOLOGIA PLANTARUM 177 . 4 (2025) . |
| APA | Miao, Chunyan , Li, Mengzhi , Li, Mingjie , Gu, Li , Zhang, Zhongyi , Gao, Li et al. Multi-Omics Landscape to Reveal the Discrepancy in the Accumulation of Flavonoids in Artemisia argyi Across Multiple Growth Stages . | PHYSIOLOGIA PLANTARUM , 2025 , 177 (4) . |
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本发明提供了一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法，属于药材鉴别技术领域。本发明包括以下步骤：采集不同太子参的高光谱图像数据；对采集的太子参高光谱图像数据进行黑白板校正，计算校正高光谱数据的相对反射率；对所述相对反射率数据进行阈值分割，提取目标感兴趣区域，计算感兴趣区域平均光谱值；剔除感兴趣区域平均光谱值的异常数据，获得原始全波段光谱数据；对原始全波段光谱数据进行特征波段筛选，将筛选获得的特征波段与种质信息利用粒子优化算法构建太子参种质鉴别模型。本发明利用高光谱成像技术构建的模型可用于太子参的分类鉴别，分辨率和灵敏度高，重复性好，简便快捷，具有广泛的应用价值。
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| GB/T 7714 | 张婷婷 , 童金鹏 , 古力 et al. 一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法 : CN202510280748.X[P]. | 2025-03-11 . |
| MLA | 张婷婷 et al. "一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法" : CN202510280748.X. | 2025-03-11 . |
| APA | 张婷婷 , 童金鹏 , 古力 , 李明杰 , 张重义 , 王衍坤 et al. 一种太子参种质鉴别模型的构建方法及太子参种质鉴别方法 : CN202510280748.X. | 2025-03-11 . |
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BACKGROUND Rehmannia glutinosa, a herb of significant medicinal and food value, is highly susceptible to pathogen infections. Fusarium oxysporum f.sp. R. glutinosa (FORg), a pathogenic strain isolated from R. glutinosa, induces root rot disease, leading to severe yield losses. Currently, resistant cultivars and effective control strategies for R. glutinosa root rot remain very limited. This study aims to investigate the feasibility and potential of SIGS technology in preventing and controlling R. glutinosa root rot caused by FORg.RESULTS This study demonstrates that FORg hyphae can capably take up dsRNA, confirming their suitability for SIGS-based disease control. dsRNA targeting SGE1 and CYP51, synthesized in vivo and extracted through low-cost ethanol method, significantly suppressed the expression of virulence-associated genes in both hyphae and conidia. Root disc assays further validated that exogenous dsRNA effectively attenuated FORg pathogenicity. Combined applications of SGE1 and CYP51S dsRNAs synergistically reduced disease severity compared to single-target treatments. Additionally, dsRNA encapsulated in layered double hydroxide (LDH) prolonged functional durability, with sustained plant protection observed for 15 days post-spraying, outperforming unencapsulated dsRNA.CONCLUSION This finding establishes the feasibility of SIGS for controlling FORg-induced root rot in R. glutinosa and highlights the potential of nanomaterial encapsulation in addressing persistent fungal epidemics in agricultural systems. (c) 2025 Society of Chemical Industry.
Keyword ：
disease controlling disease controlling fungal uptake fungal uptake nanocarriers nanocarriers spray-induced gene silencing spray-induced gene silencing
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| GB/T 7714 | Guo, Bingyang , Yang, Yanhui , Tan, Jiayi et al. NanoClay-enhanced spray-induced gene silencing as a biological control strategy for Rehmannia glutinosa root rot disease [J]. | PEST MANAGEMENT SCIENCE , 2025 . |
| MLA | Guo, Bingyang et al. "NanoClay-enhanced spray-induced gene silencing as a biological control strategy for Rehmannia glutinosa root rot disease" . | PEST MANAGEMENT SCIENCE (2025) . |
| APA | Guo, Bingyang , Yang, Yanhui , Tan, Jiayi , Wang, Rong , Xue, Tianrui , Li, Mingjie et al. NanoClay-enhanced spray-induced gene silencing as a biological control strategy for Rehmannia glutinosa root rot disease . | PEST MANAGEMENT SCIENCE , 2025 . |
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Accurate authentication of Pseudostellaria heterophylla (TZS) is essential for quality control in traditional Chinese medicine (TCM). This study pioneers a non-destructive authentication framework by systematically comparing machine vision and hyperspectral imaging (HSI) across visible-near-infrared and short-wave infrared (SWIR) spectral ranges. Multiple classification algorithms, including support vector machines, partial least squares discriminant analysis, extreme learning machines, and backpropagation neural networks (BPNN), were implemented. Wavelength selection was performed using competitive adaptive reweighted sampling (CARS), successive projections algorithm (SPA), and their hybrid approach, while model parameters were optimized through particle swarm optimization (PSO). Results revealed that the SWIR HSI system achieved 96.4 % classification accuracy using a CARS-SPA-PSO-BPNN model with only 16 effective wavelengths in 13.9 s, accomplishing 95.9 % spectral dimensionality reduction while maintaining optimal performance. Complementary untargeted metabolomic profiling via UHPLC-Q-TOF/MS identified 41 key differential metabolites, including fatty acids, lipids, cyclic peptides, and terpenoids, correlated with the spectral features, mechanistically validating the model's decisions. This work lays the foundation for a streamlined hyperspectral sorting system to detect the authenticity of TZS, advancing TCM standardization and industrialization.
Keyword ：
Hyperspectral imaging Hyperspectral imaging Non-destructive authentication Non-destructive authentication Pseudostellaria heterophylla Pseudostellaria heterophylla TCM quality control TCM quality control UHPLC-Q-TOF/MS UHPLC-Q-TOF/MS
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| GB/T 7714 | Zhang, Tingting , Tong, Jinpeng , Li, Long et al. Integration of SWIR hyperspectral imaging, UHPLC-Q-TOF/MS and optimized machine learning for non-destructive authentication of Pseudostellaria heterophylla [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 234 . |
| MLA | Zhang, Tingting et al. "Integration of SWIR hyperspectral imaging, UHPLC-Q-TOF/MS and optimized machine learning for non-destructive authentication of Pseudostellaria heterophylla" . | INDUSTRIAL CROPS AND PRODUCTS 234 (2025) . |
| APA | Zhang, Tingting , Tong, Jinpeng , Li, Long , Yuan, Jiduan , Yuan, Xiaotan , Yuan, Feiyue et al. Integration of SWIR hyperspectral imaging, UHPLC-Q-TOF/MS and optimized machine learning for non-destructive authentication of Pseudostellaria heterophylla . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 234 . |
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The obstacle of continuous cropping poses a key challenge limiting the sustainable and safe production of Chinese herbal medicines, with the dysregulation of plant immune responses being identified as the primary contributing factor. LRR-RLPs, as major members of plant pattern recognition receptors (PRRs), are extensively involved in mediating plant immune responses. However, these receptors remain unidentified in the medicinal plant Rehmannia glutinosa. In this study, we identified 14 RgRLP genes in R. glutinosa through bioinformatics approaches and systematically analyzed their differential expression patternas under potted cultivation versus controlled simulated continuous cropping conditions. Subsequently, we constructed overexpression systems for functional characterization of three pivotal RgRLP genes (RgRLP4, RgRLP10, and RgRLP12), which were identified as central regulators of continuous cropping stress responses. Notably, continuous cropping stress induced severe oxidative damage in root tip tissues and promoted rhizosphere proliferation of Fusarium oxysporum via exudate-mediated interactions, thereby exacerbating stress effects. Importantly, overexpression of RgRLP genes significantly alleviated continuous cropping-induced stress damage. Furthermore, transcriptomic analysis of transgenic lines revealed marked upregulation of three LRR-RLK genes (RgRLK19, RgRLK27, and RgRLK30), suggesting their potential roles in RgRLP-mediated stress signaling pathways. This study provides a good theoretical basis and technical support for in-depth study of the formation mechanism and reduction strategies of continuous cropping obstacles of R. glutinosa.
Keyword ：
Continuous cropping obstacles Continuous cropping obstacles F. oxysporum F. oxysporum LRR-RLP genes LRR-RLP genes Rehmannia glutinosa Rehmannia glutinosa
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| GB/T 7714 | Qiu, Fuxiang , Yu, Zhijian , Fan, Yongxi et al. Screening and functional characterization of Rehmannia glutinosa receptor proteins (RgRLPs) in response to continuous cropping stress [J]. | SCIENTIA HORTICULTURAE , 2025 , 344 . |
| MLA | Qiu, Fuxiang et al. "Screening and functional characterization of Rehmannia glutinosa receptor proteins (RgRLPs) in response to continuous cropping stress" . | SCIENTIA HORTICULTURAE 344 (2025) . |
| APA | Qiu, Fuxiang , Yu, Zhijian , Fan, Yongxi , Zhang, Bao , Zhang, Guojun , Lai, Yanlin et al. Screening and functional characterization of Rehmannia glutinosa receptor proteins (RgRLPs) in response to continuous cropping stress . | SCIENTIA HORTICULTURAE , 2025 , 344 . |
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Anoectochilus roxburghii (Wall.) Lindl. (A. roxburghii) is an increasingly popular medicinal herb. Arbuscular mycorrhiza (AM) fungi, known for their symbiotic relationships with plant roots, enhance nutrient uptake and disease resistance in host plants. However, their specific regulatory mechanisms in A. roxburghii are not fully understood. In this study, Fujian A. roxburghii was inoculated with the AM fungus Glomus intraradices, and successful root colonization was observed. Following AM fungal colonization, there was a significant upregulation of photosynthesis-related genes in the stems, accompanied by improved canopy phenotypes and root architecture. Consequently, AM-inoculated plants exhibited increased fresh and dry biomass, as well as elevated levels of polysaccharides and flavonoids. Additionally, the incidence of Fusarium oxysporum-induced stalk rot was reduced in AM-inoculated plants. Analysis of defense-related enzymes indicated that AM-inoculated plants exhibited a rapid and robust response to pathogen infection, mitigating oxidative stress. Transcriptomic analysis revealed significant upregulation of genes associated "Fatty acid degradation", "MAPK signaling pathway-plant", and "Plant-pathogen interaction", suggesting their involvement in enhanced disease resistance. A regulatory network centered on ACX1 and calmodulin, involving multiple transcription factors such as WRKY, bHLH, ERF, NAC, and HSF, was implicated in defense responses. These findings demonstrated the beneficial effects of AM fungi on yield, quality, and disease resistance in A. roxburghii, providing a theoretical foundation for its cultivation and genetic improvement.
Keyword ：
Anoectochilus roxburghii Anoectochilus roxburghii Arbuscular mycorrhiza fungus Arbuscular mycorrhiza fungus Bioactive compound Bioactive compound Biomass accumulation Biomass accumulation Disease resistance Disease resistance
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| GB/T 7714 | Gu, Li , Li, Shurong , Zhou, Lichun et al. Ecophysiological and transcriptional landscapes of arbuscular mycorrhiza fungi enhancing yield, quality, and stalk rot resistance in Anoectochilus roxburghii [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 . |
| MLA | Gu, Li et al. "Ecophysiological and transcriptional landscapes of arbuscular mycorrhiza fungi enhancing yield, quality, and stalk rot resistance in Anoectochilus roxburghii" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 223 (2025) . |
| APA | Gu, Li , Li, Shurong , Zhou, Lichun , Yuan, Feiyue , Zhang, Tingting , Wang, Yankun et al. Ecophysiological and transcriptional landscapes of arbuscular mycorrhiza fungi enhancing yield, quality, and stalk rot resistance in Anoectochilus roxburghii . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 . |
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喜阳植物感知群体临近或冠层荫蔽条件权衡生长和防御的变化已被深刻理解，但耐荫植物在长期进化中产生的适应性特征尚不清楚。该研究以典型的阴生中药材金线莲为材料，设置(1)不同的种植密度，包括8 cm(行距)×8 cm(株距)、6 cm×6 cm、4 cm×4 cm、2 cm×2 cm,以监测植株个体感知邻近遮荫的响应特征；(2)不同远红光的环境，包括蓝光-红光=3∶2(B3R2)、蓝光-红光-远红光=3∶2∶1(B3R2FR1)、蓝光-红光-远红光=3∶2∶2(B3R2FR2)和蓝光-红光-远红光=3∶2∶4(B3R2FR4),以监测植物响应实际遮荫的变化趋势。结果显示：(1)适度增加种植密度有助于金线莲茎粗和叶面积等形态指标的优化，小幅度提高生物量的同时显著提高叶、茎的超氧化物歧化酶(SOD)活性和茎部木质素含量，增强防御能力；(2)光线中远红光增加负调控金线莲株高，与喜阳植物中典型的避荫响应相反，但显著提高茎、叶中SOD和过氧化物酶(POD)活性以及茎部木质素含量，降低茎腐病的发病率和病情指数，实现了对生物胁迫的有效防御。因此，耐荫植物金线莲对荫蔽条件具有特异性适应策略，合理密植或调配光环境可协同改善金线莲的产量、品质和抗性，该研究为中药材生态种植的区域部署和栽培策略优化奠定理论基础与技术支撑。
Keyword ：
抗病性 抗病性 生物量 生物量 种植密度 种植密度 荫蔽条件 荫蔽条件 药用品质 药用品质 远红光 远红光 金线莲 金线莲
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| GB/T 7714 | 郭晓磊 , 周礼春 , 李明杰 et al. 耐荫植物金线莲对密植或光质调配形成荫蔽条件的形态学和生理学响应 [J]. | 中国中药杂志 , 2025 , 50 (10) : 2648-2657 . |
| MLA | 郭晓磊 et al. "耐荫植物金线莲对密植或光质调配形成荫蔽条件的形态学和生理学响应" . | 中国中药杂志 50 . 10 (2025) : 2648-2657 . |
| APA | 郭晓磊 , 周礼春 , 李明杰 , 张重义 , 古力 . 耐荫植物金线莲对密植或光质调配形成荫蔽条件的形态学和生理学响应 . | 中国中药杂志 , 2025 , 50 (10) , 2648-2657 . |
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Pseudostellaria heterophylla (Miq.) Pax (P. heterophylla) was a valued traditional Chinese herbal medicine. Previous studies have shown that P. heterophylla TuMV spreads during the vegetative propagation cycle using tuberous roots as carriers. However, the transmission mechanism of TuMV in P. heterophylla and its effects on host growth remain to be elucidated. In this study, virus-free P. heterophylla culture seedlings were infected with control, TuMV-ZR, and TuMV-ZR-EGFP, thereby resulting in the initial infection cycle of IF1 (TIF1, TEIF1) and control NIF1, and used these roots to propagate the subsequent infection cycle IF2 (TIF2, TEIF2) and control NIF2. The transmission of TuMV-ZR seedlings was tracked by EGFP signal, and their yield, quality, and resistance were analyzed simultaneously in the critical growth period of the plants. The results indicated that TuMV-ZR accumulated in the tuberous roots of IF1 plants, subsequently migrated to IF2 during seedling growth, and was re-stored in IF2 tuberous roots, thereby forming a simple virus transmission cycle. Meanwhile, the tuberous roots of IF1 and IF2 P. heterophylla showed lower fresh weight, dry weight, soluble sugar, and saponin levels compared to NIF1 and NIF2, respectively. TuMV caused a significant reduction in chlorophyll synthesis in IF1 and IF2 P. heterophylla, resulting in impairment to their photosynthetic organs and efficiency. The measurement of stress resistance in IF1 and IF2 P. heterophylla revealed that continuous viral infection disrupted antioxidant enzyme activity, increased the content of MDA, enhanced the activity of PAL, and elevated the levels of intracellular osmotic substances in both propagation cycles. The findings indicated that the accumulation of the TuMV-ZR virus during two successive vegetative propagation cycles induced physiological stress, impaired photosynthesis, and caused progressive yield and quality decline with each cycle. This study systematically examined the impact of TuMV-ZR persistence during vegetative propagation on key physiological and biochemical indices in P. heterophylla, providing critical data to clarify vegetative-propagation-mediated germplasm degradation.
Keyword ：
P. heterophylla P. heterophylla quality quality stress resistance stress resistance TuMV-ZR virus TuMV-ZR virus vegetative propagation cycles vegetative propagation cycles yield yield
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| GB/T 7714 | Gu, Li , Qian, Sheng , Yao, Shuting et al. Long-Term Effects of Vegetative-Propagation-Mediated TuMV-ZR Transmission on Yield, Quality, and Stress Resistance in Pseudostellaria heterophylla [J]. | PATHOGENS , 2025 , 14 (4) . |
| MLA | Gu, Li et al. "Long-Term Effects of Vegetative-Propagation-Mediated TuMV-ZR Transmission on Yield, Quality, and Stress Resistance in Pseudostellaria heterophylla" . | PATHOGENS 14 . 4 (2025) . |
| APA | Gu, Li , Qian, Sheng , Yao, Shuting , Wu, Jiaxin , Wang, Lianghong , Mu, Jing et al. Long-Term Effects of Vegetative-Propagation-Mediated TuMV-ZR Transmission on Yield, Quality, and Stress Resistance in Pseudostellaria heterophylla . | PATHOGENS , 2025 , 14 (4) . |
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Rehmannia glutinosa is an important medicinal herb; but its long-term cultivation often leads to continuous cropping problems. The underlying cause can be attributed to the accumulation of and alterations in root exudates; which interact with soil-borne pathogens; particularly Fusarium oxysporum; triggering disease outbreaks that severely affect its yield and quality. It is therefore crucial to elucidate the mechanisms by which root exudates induce F. oxysporum CCS043 outbreaks. In this study; the genome of F. oxysporum CCS043 from R. glutinosa's rhizosphere microbiota was sequenced and assembled de novo; resulting in a 47.67 Mb genome comprising 16,423 protein-coding genes. Evolutionary analysis suggests that different F. oxysporum strains may adapt to the host rhizosphere microecosystem by acquiring varying numbers of specific genes while maintaining a constant number of core genes.The allelopathic effects of ferulic acid; verbascoside; and catalpol on F. oxysporum CCS043 were examined at the physiological and transcriptomic levels. The application of ferulic acid was observed to primarily facilitate the proliferation and growth of F. oxysporum CCS043; whereas verbascoside notably enhanced the biosynthesis of infection-related enzymes such as pectinase and cellulase. Catalpol demonstrated a moderate level of allelopathic effects in comparison to the other two. Furthermore; 10 effectors were identified by combining the genomic data. Meanwhile; it was found that among the effector-protein-coding genes; ChiC; VRDA; csn; and chitinase exhibited upregulated expression across all treatments. The expression patterns of these key genes were validated using qRT-PCR. Transient overexpression of the two effector-encoding genes in detached R. glutinosa leaves provided further confirmation that ChiC (GME8876_g) and csn (GME9251_g) are key effector proteins responsible for the induction of hypersensitive reactions in R. glutinosa leaf cells. This study provides a preliminary indication that the use of allelochemicals by F. oxysporum CCS043 can promote its own growth and proliferation and enhance infection activity. This finding offers a solid theoretical basis and data support for elucidating the fundamental causes of fungal disease outbreaks in continuous cropping of R. glutinosa and for formulating effective mitigation strategies.
Keyword ：
allelopathy allelopathy effector protein effector protein F. oxysporum CCS043 F. oxysporum CCS043 Rehmannia glutinosa Rehmannia glutinosa root exudates root exudates
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| GB/T 7714 | Yuan, Feiyue , Qiu, Fuxiang , Xie, Jiawei et al. Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa [J]. | PLANTS-BASEL , 2025 , 14 (1) . |
| MLA | Yuan, Feiyue et al. "Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa" . | PLANTS-BASEL 14 . 1 (2025) . |
| APA | Yuan, Feiyue , Qiu, Fuxiang , Xie, Jiawei , Fan, Yongxi , Zhang, Bao , Zhang, Tingting et al. Mechanism of Action of Fusarium oxysporum CCS043 Utilizing Allelochemicals for Rhizosphere Colonization and Enhanced Infection Activity in Rehmannia glutinosa . | PLANTS-BASEL , 2025 , 14 (1) . |
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The Agrobacterium tumefaciens mediated transformation is the prevailing methodology for plant genetic manipulation; however, A. tumefaciens overgrowth is a common constraint in the process. Exploring auxotrophic A. tumefaciens could reduce overgrowth and enhance plant transformation efficiency. The ILVC gene, which encodes the ketol-acid isomeroreductase, is critical for Valine (Val) and isoleucine (Ile) biosynthesis in some microorganisms. However, its function in A. tumefaciens is unclear. To ascertain the function of ILVC and generate an auxotrophic A. tumefaciens, this study employed an allelic exchange to disrupt the ILVC in A. tumefaciens strain GV3101. This resulted in the loss of ketol-acid isomeroreductase activity and the prevention of Val and Ile biosynthesis, creating a dual-auxotrophic GV3101 triangle ILVC. Transient expression assays in Nicotiana benthamiana transformation demonstrated that the GV3101 triangle ILVC was capable of T-DNA transfer. Moreover, stable genetic transformation analysis in N. benthamiana indicated that the introduction of GV3101 triangle ILVC led to a reduction in overgrowth within infected plant tissues. Additionally, an enhancement in transformation efficiency was observed with the prolongation of the co-cultivation time of the explant-infected strain. This study revealed the function of ILVC and explored a dual-auxotrophic A. tumefaciens for Val and Ile, potentially broadening the utilization of auxotrophic strains in plant genetic transformation.
Keyword ：
Agrobacterium tumefaciens Agrobacterium tumefaciens Auxotrophy Auxotrophy ILVC gene ILVC gene Plant transformation Plant transformation Valine and isoleucine biosynthesis Valine and isoleucine biosynthesis
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| GB/T 7714 | Yang, Yanhui , Lu, Jun , Xue, Tianrui et al. Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation [J]. | TRANSGENIC RESEARCH , 2025 , 34 (1) . |
| MLA | Yang, Yanhui et al. "Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation" . | TRANSGENIC RESEARCH 34 . 1 (2025) . |
| APA | Yang, Yanhui , Lu, Jun , Xue, Tianrui , Cao, Zhenli , Li, Mingjie , Zhang, Zhongyi et al. Knockout of the Agrobacterium ILVC gene generates a valine-isoleucine auxotrophic strain for plant transformation . | TRANSGENIC RESEARCH , 2025 , 34 (1) . |
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