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学者姓名:宋圆圆

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< Page ,Total 12 >
A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance SCIE
期刊论文 | 2025 , 246 (5) , 2243-2262 | NEW PHYTOLOGIST
WoS CC Cited Count: 4
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Abstract :

Rice plants synthesize a unique group of diterpenoid phytoalexins (DPs) that exhibit broad-spectrum antimicrobial activities and are biosynthesized by enzymes encoded by three biosynthetic gene clusters. However, the regulatory mechanisms of their biosynthesis remain unclear. Here, the regulatory roles of the transcription factor OsWRKY10 and its interacting VQ motif-containing protein OsVQ8 in DPs biosynthesis and disease resistance were investigated via genetic and biochemical analyses. Their CRISPR/Cas9-mediated knockout and over-expressing (OE) lines, as well as crossed lines WRKY10OE/vq8, were generated. OsVQ8 phosphorylation by mitogen-activated protein kinase (MAPK) cascades was examined. We found that OsWRKY10 co-expresses with and activates a specific set of genes involved in DPs biosynthesis, thereby enhancing DPs accumulation and disease resistance against both fungal blast and bacterial blight. We demonstrate that OsWRKY10 interacts with the VQ motif-containing protein OsVQ8, modulating DPs biosynthesis through OsVQ8 phosphorylation by the activated OsMKK4-OsMPK6 cascade upon perception of pathogen-associated molecular patterns. Our findings highlight how the interaction between OsVQ8 and OsWRKY10 serves as a molecular switch to regulate gene clusters and the entire pathway of DPs biosynthesis in rice and provides valuable insights for genetic engineering aimed at enhancing phytoalexin production and broad-spectrum disease resistance in staple food crops.

Keyword :

disease resistance disease resistance diterpenoid phytoalexins diterpenoid phytoalexins Oryza sativa L. Oryza sativa L. OsMKK4 OsMKK4 OsMPK6 OsMPK6 OsVQ8 OsVQ8 OsWRKY10 OsWRKY10

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GB/T 7714 Lin, Xianhui , Ding, Chaohui , Xiao, Wei et al. A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance [J]. | NEW PHYTOLOGIST , 2025 , 246 (5) : 2243-2262 .
MLA Lin, Xianhui et al. "A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance" . | NEW PHYTOLOGIST 246 . 5 (2025) : 2243-2262 .
APA Lin, Xianhui , Ding, Chaohui , Xiao, Wei , Wang, Jinhao , Lin, Zhuo , Sun, Xinli et al. A molecular switch OsWRKY10-OsVQ8 orchestrates rice diterpenoid phytoalexin biosynthesis for broad-spectrum disease resistance . | NEW PHYTOLOGIST , 2025 , 246 (5) , 2243-2262 .
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A simple and efficient TALEN system for genome editing in plants SCIE
期刊论文 | 2025 , 115 (1) | PLANT MOLECULAR BIOLOGY
WoS CC Cited Count: 1
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Abstract :

TALEN-mediated plant genome editing has the advantages of high specificity, independence from epigenetic modification, lack of PAM limitation, and feasibility for organelle editing due to no need for RNA binding. However, the assembly of TALEN vectors was difficult and laborious, affecting the popularity of TALEN genome editing in plants. In this study, a novel TALEN-based plant genome editing tool, ZQTALEN, was developed. This system comprises a total of nine plasmids categorized into three types. The TALE repeat units are obtained through PCR utilizing the template vector as the amplification template. These units are then assembled sequentially: first into donor vectors to form entry vectors, and subsequently transferred to destination vectors, resulting in the final binary vector. Characterized by the optimization of codon usage, assembly method of TALE repeat array, and vector backbone components, ZQTALEN has the advantages of easy, flexible and efficient assembly and less repeated sequences in the final vector. Using the ZQTALEN system, a TALEN binary vector was successfully constructed to target the endogenous Nramp5 gene in rice, resulting in the high-frequency acquisition of rice mutants. The ZQTALEN system has provided a versatile tool for genetic research in plants.

Keyword :

Gateway recombination Gateway recombination Genome editing Genome editing Golden gate cloning Golden gate cloning RVD (Repeat variant di-residue) RVD (Repeat variant di-residue) TALEN (Transcription activator-like effector nuclease) TALEN (Transcription activator-like effector nuclease)

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GB/T 7714 Liu, Qing , Lin, Lizhou , He, Shengjian et al. A simple and efficient TALEN system for genome editing in plants [J]. | PLANT MOLECULAR BIOLOGY , 2025 , 115 (1) .
MLA Liu, Qing et al. "A simple and efficient TALEN system for genome editing in plants" . | PLANT MOLECULAR BIOLOGY 115 . 1 (2025) .
APA Liu, Qing , Lin, Lizhou , He, Shengjian , Yu, Jianghui , Xie, Caili , Gai, Chaoyue et al. A simple and efficient TALEN system for genome editing in plants . | PLANT MOLECULAR BIOLOGY , 2025 , 115 (1) .
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BAHD acyltransferase OsSLG mediates rice cadmium tolerance by integrating the brassinosteroid and salicylic acid pathway SCIE
期刊论文 | 2025 , 356 | PLANT SCIENCE
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Cadmium (Cd) is a highly toxic element that significantly threatens plant growth and human health. Brassinosteroids (BRs) and salicylic acid (SA) are crucial phytohormones involved in plant growth and defense. While the mechanisms by which BRs and SA individually regulate various plant biological processes have been extensively studied, their interaction with Cd in rice (Oryza sativa L.) remains poorly understood. In this study, we demonstrated that SLENDER GRAIN (OsSLG), a BR biosynthesis-related gene, plays a critical role in regulating in rice. Overexpression of OsSLG enhanced Cd tolerance, whereas OsSLG RNA interference (RNAi) lines (OsSLG-Ri) exhibited hypersensitivity to Cd stress. Exogenous BR treatment improved the Cd tolerance of the wild type and rescued the Cd-sensitive phenotype of OsSLG-Ri. Furthermore, OsSLG overexpression significantly reduced reactive oxygen species (ROS) and Cd accumulation, this reduction was attributed to the downregulation of genes involved in Cd absorption and transport, as well as the upregulation of genes associated with Cd detoxification and ROS scavenging. In addition, OsSLG enhanced the photosynthetic capacity and mineral element content in rice plants, improving their ability to cope with Cd stress. Gene expression analysis showed that OsSLG promoted the expression of the SA pathway genes, and phenotypic analysis confirmed that SA positively regulates Cd tolerance in rice. Notably, BR-induced Cd tolerance was diminished in SA biosynthesis-deficient rice plants overexpressing SA hydroxylase genes OsS5H1 and OsS5H2, suggesting that the SA pathway is necessary for BR-mediated Cd tolerance. In conclusion, our findings highlight OsSLG as a key player in elucidating the interplay between BR and SA under Cd stress.

Keyword :

Brassinosteroids Brassinosteroids Cd tolerance Cd tolerance OsSLG OsSLG ROS scavenging ROS scavenging Salicylic acid Salicylic acid

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GB/T 7714 Zhang, Zaoli , Li, Long , Qiu, Shunjiao et al. BAHD acyltransferase OsSLG mediates rice cadmium tolerance by integrating the brassinosteroid and salicylic acid pathway [J]. | PLANT SCIENCE , 2025 , 356 .
MLA Zhang, Zaoli et al. "BAHD acyltransferase OsSLG mediates rice cadmium tolerance by integrating the brassinosteroid and salicylic acid pathway" . | PLANT SCIENCE 356 (2025) .
APA Zhang, Zaoli , Li, Long , Qiu, Shunjiao , Sun, Yanyan , Zhang, Rongjun , Chen, Dongmei et al. BAHD acyltransferase OsSLG mediates rice cadmium tolerance by integrating the brassinosteroid and salicylic acid pathway . | PLANT SCIENCE , 2025 , 356 .
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Maize Herbivore-Induced Volatiles Enhance Xenobiotic Detoxification in Larvae of Spodoptera frugiperda and S. litura SCIE
期刊论文 | 2025 , 14 (1) | PLANTS-BASEL
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Abstract :

The release of herbivore-induced plant volatiles (HIPVs) has been recognized to be an important strategy for plant adaptation to herbivore attack. However, whether these induced volatiles are beneficial to insect herbivores, particularly insect larvae, is largely unknown. We used the two important highly polyphagous lepidopteran pests Spodoptera frugiperda and S. litura to evaluate the benefit on xenobiotic detoxification of larval exposure to HIPVs released by the host plant maize (Zea mays). Larval exposure of the invasive alien species S. frugiperda to maize HIPVs significantly enhanced their tolerance to all three of the well-known defensive compounds 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA), chlorogenic acid, and tannic acid in maize and the two commonly used insecticides methomyl and chlorpyrifos. HIPV exposure also improved the larval tolerance of S. litura third instars to chlorogenic and tannic acids. Furthermore, larval exposure to either maize HIPVs or DIMBOA induced the activities of cytochrome P450 enzymes (P450s), glutathione-s-transferase (GST), and carboxylesterase (CarE) in the midguts and fat bodies of the two insects, while the induction was significantly higher by the two components together. In addition, the expression of four genes encoding uridine diphosphate (UDP)-glycosyltransferases (UGT33F28, UGT40L8) and P450s (CYP4d8, CYP4V2) showed similar induction patterns in S. frugiperda. Cis-3-hexen-1-ol, an important component in maize HIPVs, also showed the same functions as maize HIPVs, and its exposure increased larval xenobiotic tolerance and induced the detoxification enzymes and gene expression. Our findings demonstrate that HIPVs released by the pest-infested host plants are conductive to the xenobiotic tolerance of lepidopteran insect larvae. Hijacking the host plant HIPVs is an important strategy of the invasive alien polyphagous lepidopteran pest to counter-defend against the host plant's chemical defense.

Keyword :

cytochrome P450 cytochrome P450 DIMBOA DIMBOA herbivore-induced plant volatiles herbivore-induced plant volatiles Spodoptera frugiperda Spodoptera frugiperda xenobiotic xenobiotic

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GB/T 7714 Wang, Peng , Zeng, Qiyue , Zhao, Yi et al. Maize Herbivore-Induced Volatiles Enhance Xenobiotic Detoxification in Larvae of Spodoptera frugiperda and S. litura [J]. | PLANTS-BASEL , 2025 , 14 (1) .
MLA Wang, Peng et al. "Maize Herbivore-Induced Volatiles Enhance Xenobiotic Detoxification in Larvae of Spodoptera frugiperda and S. litura" . | PLANTS-BASEL 14 . 1 (2025) .
APA Wang, Peng , Zeng, Qiyue , Zhao, Yi , Sun, Xiaomin , Han, Yongqiang , Zeng, Rensen et al. Maize Herbivore-Induced Volatiles Enhance Xenobiotic Detoxification in Larvae of Spodoptera frugiperda and S. litura . | PLANTS-BASEL , 2025 , 14 (1) .
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Dual Localization and Functional Divergence of V-ATPase Subunit A: Nuclear Shuttling Mediates Distinct Roles in Dark- and MeJA-Induced Leaf Senescence SCIE
期刊论文 | 2025 , 73 (17) , 10157-10171 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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Abstract :

Retrograde signaling regulates plant senescence, but the role of vacuoles in this process remains unclear. Here, we demonstrate that rice vacuolar H+-ATPase subunit A (OsVHA-A) localizes to both the cytoplasm and nucleus. Sucrose treatment increased OsVHA-A expression and nuclear accumulation, while darkness reduced it. Methyl jasmonate (MeJA) initially promoted OsVHA-A nuclear translocation but decreased it upon prolonged exposure. Downregulation of OsVHA-A expression accelerated MeJA-induced rice leaf senescence but delayed darkness-induced senescence. MeJA treatment also significantly upregulated the expression of OsMYC2 and OsMAPK6 in OsVHA-A-RNAi plants compared to wild-type plants. Moreover, OsVHA-A downregulation notably increased the level of expression of genes associated with sugar signaling and transport under dark conditions. Immunoprecipitation-mass spectrometry and molecular docking analyses identified interactions between OsVHA-A and OsTPR1, OsMed14, sucrose transporters, and enzymes involved in sucrose metabolism. The binding of OsVHA-A with OsTPR1 and OsSUS1 was confirmed by BiFC. These findings highlight the multifunctional role of OsVHA-A in coordinating organelles and nuclear signaling, providing new insights and potential strategies for manipulating senescence to improve rice yield and quality.

Keyword :

jasmonic acid jasmonic acid leaf senescence leaf senescence OsVHA-A OsVHA-A retrograde signal retrograde signal vacuole vacuole

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GB/T 7714 Wang, Yifan , Lei, Jianhui , Liu, Jian et al. Dual Localization and Functional Divergence of V-ATPase Subunit A: Nuclear Shuttling Mediates Distinct Roles in Dark- and MeJA-Induced Leaf Senescence [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (17) : 10157-10171 .
MLA Wang, Yifan et al. "Dual Localization and Functional Divergence of V-ATPase Subunit A: Nuclear Shuttling Mediates Distinct Roles in Dark- and MeJA-Induced Leaf Senescence" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 17 (2025) : 10157-10171 .
APA Wang, Yifan , Lei, Jianhui , Liu, Jian , Gao, Mengyu , Cheng, Junyi , Chang, Jun et al. Dual Localization and Functional Divergence of V-ATPase Subunit A: Nuclear Shuttling Mediates Distinct Roles in Dark- and MeJA-Induced Leaf Senescence . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (17) , 10157-10171 .
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Metabolomic profiling reveals the anti-herbivore mechanism of rice (Oryza sativa) induced by silicon SCIE
期刊论文 | 2025 , 19 (1) | ARTHROPOD-PLANT INTERACTIONS
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Abstract :

Annual loss of rice caused by insect pests accounts for about 30% of total production, and the use of traditional pesticides has brought about environmental pollution, food safety, and other problems. The use of secondary metabolites of rice to control pests has become a research hotspot, but little is known about the mechanism of rice self-resistance. Rice is a typical silicon accumulating crop. Previous study showed that silicon can significantly enhance the resistance of rice to Chilo suppressalis, but anti-insect active substances in silicon-treated rice were unknown. In current study, metabolomics analysis has been performed on two groups of rice (T1, treated with silicon and insect; T3, treated with only insect). A total of 151 significantly different metabolites were obtained, compared with T3 group, 23 metabolites in T1 were significantly up-regulated and 128 metabolites were significantly down-regulated. Different metabolites were mainly enriched to tryptophan metabolism, lipoic acid metabolism, linoleic acid metabolism, isoflavone biosynthesis, and indole alkaloid biosynthesis. The different metabolites (p < 0.1) enriched to lipoic acid metabolism and fatty acid biosynthesis were all significantly up-regulated. Ten significantly up-regulated different metabolites were selected from T1. These were 3-hydroxy-palmitic acid methyl ester (1), octanoic acid (2), 3-hydroxyoctadecanoic acid (3), 12-hydroxyoctadecanoic acid (4), 2-linoleoylglycerol (5), methyleugenol (6), alpha-asarone (7), 2,4,5-trimethoxybenzaldehyde (8), acitretin (9), and menatetrenone (10), and their anti-insect activity was evaluated. Compounds 1-5 and 7-10 could significantly inhibit the growth of Chilo suppressalis. Compounds 2 and 3 inhibited growth of the insect by 35.5 and 64.5%, respectively. Compound 2 belongs to lipoic acid metabolism and fatty acid biosynthesis. We speculate that silicon positively regulated the metabolic pathway of lipoic acid and fatty acid to enhance the resistance of rice to insects.

Keyword :

Anti-insect active substances Anti-insect active substances Metabolomics Metabolomics Oryza sativa Oryza sativa Silicon induce Silicon induce

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GB/T 7714 Gu, Chengzhen , Wang, Mengmeng , Lin, Yangzheng et al. Metabolomic profiling reveals the anti-herbivore mechanism of rice (Oryza sativa) induced by silicon [J]. | ARTHROPOD-PLANT INTERACTIONS , 2025 , 19 (1) .
MLA Gu, Chengzhen et al. "Metabolomic profiling reveals the anti-herbivore mechanism of rice (Oryza sativa) induced by silicon" . | ARTHROPOD-PLANT INTERACTIONS 19 . 1 (2025) .
APA Gu, Chengzhen , Wang, Mengmeng , Lin, Yangzheng , Zhang, Yujia , Khan, Afsar , Song, Yuanyuan et al. Metabolomic profiling reveals the anti-herbivore mechanism of rice (Oryza sativa) induced by silicon . | ARTHROPOD-PLANT INTERACTIONS , 2025 , 19 (1) .
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A small peptide miPEP172b encoded by primary transcript of miR172b regulates salt tolerance in rice SCIE
期刊论文 | 2025 , 219 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
WoS CC Cited Count: 1
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Abstract :

Recent studies have demonstrated that the primary transcript of miRNAs (pri-miRNAs) are able to encode small peptides influencing plant growth and development, as well as responses to various environmental cues. However, their role in plant responses to salt stress is not fully comprehended. Here, we characterized a short peptide encoded by miR172b (miPEP172b) in rice (Oryza sativa L.). By applying synthetic miPEP172b, we observed a significant increase in miR172b abundance and a decrease in the expression of its target gene IDS1. Consequently, plants treated with miPEP172b exhibited enhanced tolerance to salinity stress. Furthermore, we found that miPEP172b was efficiently absorbed by roots and transported to the aerial parts of the plant, thus conferring salt tolerance in the aboveground organs. Overexpression of miPEP172b resulted in reduced levels of reactive oxygen species (ROS), leading to improved performance of rice seedlings under salinity conditions. This was consistent with the observations in miR172-overexpressing plants. Conversely, miPEP172b mutants showed increased sensitivity to salt stress. Further analysis revealed that miPEP172b-miR172-IDS1 improved rice salt tolerance by integrating the ROS scavenging pathway and plant hormone signaling. Our findings highlight the significant role of miPEP172b in regulating miR172 activity and salt tolerance, providing a useful agent for improving crop salt tolerance.

Keyword :

miR172 miR172 miRNA-encoded peptide miRNA-encoded peptide Rice Rice ROS ROS Salt stress Salt stress

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GB/T 7714 Lu, Long , Wang, Yuan , Huang, Zecong et al. A small peptide miPEP172b encoded by primary transcript of miR172b regulates salt tolerance in rice [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 219 .
MLA Lu, Long et al. "A small peptide miPEP172b encoded by primary transcript of miR172b regulates salt tolerance in rice" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 219 (2025) .
APA Lu, Long , Wang, Yuan , Huang, Zecong , Qiu, Shunjiao , Lin, Jie , Feng, Yiran et al. A small peptide miPEP172b encoded by primary transcript of miR172b regulates salt tolerance in rice . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 219 .
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Jasmonic Acid Enhances Rice Cadmium Tolerance by Suppressing Cadmium Uptake and Translocation SCIE
期刊论文 | 2025 , 14 (7) | PLANTS-BASEL
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Worldwide, cadmium (Cd) contamination severely threatens rice production and public health. Jasmonic acid (JA) is recognized to be involved in rice Cd stress responses, but the underlying mechanism remains unclear. In this study, we show that JA positively regulates Cd tolerance in rice by repressing Cd uptake and root-to-shoot translocation. Cd exposure rapidly elevated the endogenous JA in rice roots, which was associated with increased expression of JA synthesis and JA-responsive genes. Moreover, silencing the expression of either allene oxide synthase (OsAOS; active in JA biosynthesis) or CORONATINE INSENSITIVE1 (OsCOI1; active in JA perception) resulted in aggravated Cd toxicity and increased Cd accumulation in both the roots and shoots, as well as increased translocation from the root to the shoots. A short-term uptake experiment revealed that silencing of OsAOS and OsCOI1 enhanced root Cd uptake ability. Furthermore, the elevated transcript levels of genes for Cd uptake (OsNramp5, OsNramp1, and OsIRT1) and root-to-shoot translocation (OsHMA2) were observed in OsAOS and OsCOI1 RNAi plants in comparison with wild-type plants. Taken together, our findings suggest that JA enhances rice cadmium tolerance by suppressing Cd uptake and translocation.

Keyword :

Cd tolerance Cd tolerance Cd transporter Cd transporter jasmonic acid jasmonic acid rice (Oryza sativa L.) rice (Oryza sativa L.)

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GB/T 7714 Zhang, Hao , Liu, Zhengkai , Li, Xinyu et al. Jasmonic Acid Enhances Rice Cadmium Tolerance by Suppressing Cadmium Uptake and Translocation [J]. | PLANTS-BASEL , 2025 , 14 (7) .
MLA Zhang, Hao et al. "Jasmonic Acid Enhances Rice Cadmium Tolerance by Suppressing Cadmium Uptake and Translocation" . | PLANTS-BASEL 14 . 7 (2025) .
APA Zhang, Hao , Liu, Zhengkai , Li, Xinyu , Liu, Xiaodong , Fang, Linzhi , Zeng, Rensen et al. Jasmonic Acid Enhances Rice Cadmium Tolerance by Suppressing Cadmium Uptake and Translocation . | PLANTS-BASEL , 2025 , 14 (7) .
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Ethylene-Mediated Production and Emission of Limonene Influence Brown Planthopper Preference for Rice Plants SCIE
期刊论文 | 2025 , 73 (5) , 2890-2901 | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
WoS CC Cited Count: 2
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Volatile organic compounds (VOCs) play a key role in plant communication with other organisms in the natural environment. However, the regulatory role of the phytohormone ethylene in volatile production in plants remains unclear. In this study, we demonstrated that the application of an ethylene precursor and amplification of ethylene signaling make rice plants more attractive to brown planthopper (BPH) females for feeding and oviposition. A combination of transcriptome and VOCs analyses indicated that overexpression of OsEIL1, a master transcription factor in the ethylene pathway, influences the transcript levels of several terpene synthase genes as well as the production of volatile terpenes. Further investigation revealed that the expression of the limonene synthase gene, OsTPS19, was down-regulated in OsEIL1-overexpressing rice plants, leading to a decrease in limonene production and release. Genetic analysis confirmed the essential role of limonene in the OsEIL1-mediated attractiveness of rice plants to BPH. Our findings provide new perspectives for understanding the role of ethylene signaling in volatile-mediated plant-insect interactions.

Keyword :

ethylene signaling ethylene signaling limonene limonene Oryza sativa Oryza sativa plant-insect interaction plant-insect interaction

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GB/T 7714 Lu, Long , Zhang, Rongjun , Zhang, Zaoli et al. Ethylene-Mediated Production and Emission of Limonene Influence Brown Planthopper Preference for Rice Plants [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (5) : 2890-2901 .
MLA Lu, Long et al. "Ethylene-Mediated Production and Emission of Limonene Influence Brown Planthopper Preference for Rice Plants" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 5 (2025) : 2890-2901 .
APA Lu, Long , Zhang, Rongjun , Zhang, Zaoli , Hou, Mengjiao , Zeng, Qiyue , Li, Long et al. Ethylene-Mediated Production and Emission of Limonene Influence Brown Planthopper Preference for Rice Plants . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (5) , 2890-2901 .
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sly-miR408b Targets a Plastocyanin-Like Protein to Regulate Mycorrhizal Symbiosis in Tomato SCIE
期刊论文 | 2025 , 48 (5) , 3590-3602 | PLANT CELL AND ENVIRONMENT
WoS CC Cited Count: 1
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Symbiosis between arbuscular mycorrhizal fungi and plants plays a crucial role in nutrient acquisition and stress resistance for terrestrial plants. microRNAs have been reported to participate in the regulation of mycorrhizal symbiosis by controlling the expression of their target genes. Herein, we found that sly-miR408b was significantly downregulated in response to mycorrhizal colonisation. Overexpression of sly-miR408b compromised mycorrhizal colonisation by Rhizophagus irregularis in tomato (Solanum lycopersicum) roots. A basic blue protein gene (SlBBP) was then identified as the new target gene of miR408b in tomato. The expression of membrane-located SlBBP was induced in a copper-dependent manner. Importantly, the loss function of SlBBP decreased the root mycorrhizal colonisation. Overexpression of SlBBP decreased SOD activity, which may interfere with the process of scavenging excessive reactive oxygen species (ROS). Mutation of RBOH1, which encodes ROS-producing enzymes NADPH oxidases, obviously reduced the arbuscule abundance in the mutant roots. Overall, our results provide evidence that sly-miR408b and its target gene SlBBP regulate mycorrhizal symbiosis in tomato through mediating ROS production.

Keyword :

arbuscular mycorrhiza arbuscular mycorrhiza microRNAs microRNAs ROS ROS SlBBP SlBBP sly-miR408b sly-miR408b tomato tomato

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GB/T 7714 Lin, Yibin , He, Chenling , Li, Zhenfang et al. sly-miR408b Targets a Plastocyanin-Like Protein to Regulate Mycorrhizal Symbiosis in Tomato [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (5) : 3590-3602 .
MLA Lin, Yibin et al. "sly-miR408b Targets a Plastocyanin-Like Protein to Regulate Mycorrhizal Symbiosis in Tomato" . | PLANT CELL AND ENVIRONMENT 48 . 5 (2025) : 3590-3602 .
APA Lin, Yibin , He, Chenling , Li, Zhenfang , Sun, Yingying , Tong, Lu , Chen, Xinyu et al. sly-miR408b Targets a Plastocyanin-Like Protein to Regulate Mycorrhizal Symbiosis in Tomato . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (5) , 3590-3602 .
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