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学者姓名：刘志钦
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Abstract ：
The co-contamination of arsenic (As) and cadmium (Cd) in paddy soils threatens rice safety, yet synergistic mitigation strategies using silicon (Si) and ferrous sulfate (FeSO4) remain underexplored. This study integrated hydroponic and soil pot experiments to evaluate Si-FeSO4 interactions on As/Cd accumulation and rice growth. Hydroponic trials employed 21-day-old rice seedlings exposed to 0.5 mg As(III)/Cd(II) L-1 with/without 70 mg Si L-1 and 30-70 mg Fe L-1, followed by sequential harvesting at 14 and 21 days. Soil experiments utilized co-contaminated paddy soil (50 mg As kg(-1) and 1.2 mg Cd kg(-1)) amended with Si (80 or 400 mg kg(-1)) and Fe (100 or 1000 mg kg(-1)), with pore water dynamics monitored over 120 days. Hydroponic results demonstrated that 70 mg Si L-1 combined with 30 or 70 mg Fe L-1 enhanced shoot biomass by 12-79% under As stress, while simultaneously reducing shoot As concentrations by 76-87% and Cd concentrations by 14-33%. Iron plaque induced by FeSO4 exhibited contrasting adsorption behaviors: hydroponic roots immobilized both As and Cd (p < 0.01), whereas roots in soil primarily retained Cd (p < 0.05). In soil experiments, the optimal treatment of 100 mg Fe kg(-1) and 400 mg Si kg(-1) (Fe-1 + Si-2) increased grain biomass by 54%, while reducing As and Cd concentrations by 37% and 42%, respectively. However, a higher Fe dosage (Fe-2: 1000 mg kg(-1) Fe) paradoxically increased grain Cd concentrations. Mechanistically, Si amendment elevated soil pH (Delta + 0.72), facilitating Cd immobilization, while FeSO4 lowered pH (Delta-0.07-0.53), increasing Cd mobility. A strong correlation between soluble Cd and plant uptake was observed (p < 0.01), while changes in As accumulation were unrelated to aqueous behavior. The optimized Si/Fe molar ratio of 7.95:1 effectively mitigated As and Cd co-accumulation, offering a dual-functional strategy for safe rice cultivation in contaminated soils.
Keyword ：
As-Cd co-contamination As-Cd co-contamination iron plaque iron plaque pore water pore water potentially toxic elements potentially toxic elements soil remediation soil remediation
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| GB/T 7714 | You, Yanlin , Guo, Xiaodong , Chen, Jianyu et al. Synergistic Effects of Silicon and Ferrous Sulfate on Reducing Arsenic and Cadmium Accumulation in Rice from Co-Contaminated Soil [J]. | AGRONOMY-BASEL , 2025 , 15 (6) . |
| MLA | You, Yanlin et al. "Synergistic Effects of Silicon and Ferrous Sulfate on Reducing Arsenic and Cadmium Accumulation in Rice from Co-Contaminated Soil" . | AGRONOMY-BASEL 15 . 6 (2025) . |
| APA | You, Yanlin , Guo, Xiaodong , Chen, Jianyu , Liu, Zhiqin , Cai, Qiuying , Yu, Jinyong et al. Synergistic Effects of Silicon and Ferrous Sulfate on Reducing Arsenic and Cadmium Accumulation in Rice from Co-Contaminated Soil . | AGRONOMY-BASEL , 2025 , 15 (6) . |
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Pepper (Capsicum annuum L.) suffers severe quality and yield loss from oomycete diseases caused by Phytophthora capsici. CaSGT1 was previously determined to positively regulate the immune response of pepper plants against P. capsici, but by which mechanism remains elusive. In the present study, the potential interacting proteins of CaSGT1 were isolated from pepper using a yeast two-hybrid system, among which CaARP1 was determined to interact with CaSGT1 via bimolecular fluorescence complementation (BiFC) and microscale thermophoresis (MST) assays. CaARP1 belongs to the auxin-repressed protein family, which is well-known to function in modulating plant growth. The transcriptional and protein levels of CaARP1 were both significantly induced by infection with P. capsici. Silencing of CaARP1 promotes the vegetative growth of pepper plants and attenuates its disease resistance to P. capsici, as well as compromising the hypersensitive response-like cell death in pepper leaves induced by PcINF1, a well-characterized typical PAMP from P. capsici. Chitin-induced transient expression of CaARP1 in pepper leaves enhanced its disease resistance to P. capsici, which is amplified by CaSGT1 co-expression as a positive regulator. Taken together, our result revealed that CaARP1 plays a dual role in the pepper, negatively regulating the vegetative growth and positively regulating plant immunity against P. capsici in a manner associated with CaSGT1.
Keyword ：
CaARP1 CaARP1 CaSGT1 CaSGT1 defense response defense response Phytophthora capsici Phytophthora capsici vegetable growth vegetable growth
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| GB/T 7714 | Li, Xia , Weng, Yahong , Chen, Yufeng et al. CaARP1/CaSGT1 Module Regulates Vegetative Growth and Defense Response of Pepper Plants against Phytophthora capsici [J]. | PLANTS-BASEL , 2024 , 13 (20) . |
| MLA | Li, Xia et al. "CaARP1/CaSGT1 Module Regulates Vegetative Growth and Defense Response of Pepper Plants against Phytophthora capsici" . | PLANTS-BASEL 13 . 20 (2024) . |
| APA | Li, Xia , Weng, Yahong , Chen, Yufeng , Liu, Kaisheng , Liu, Yanyan , Zhang, Kan et al. CaARP1/CaSGT1 Module Regulates Vegetative Growth and Defense Response of Pepper Plants against Phytophthora capsici . | PLANTS-BASEL , 2024 , 13 (20) . |
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为明确辣椒（Capsicum annuum）编码突触囊泡结合蛋白（Synaptotagmin,SYT）基因CaSYT1在辣椒应答疫霉侵染中可能发挥的作用，通过蛋白保守结构预测、亚细胞定位、疫霉及植物生长调节剂处理、瞬时表达、基因沉默等方法对CaSYT1的表达和功能进行了初步研究。结果表明：CaSYT1含有1个跨膜螺旋结构域和2个C2结构域，定位在细胞膜；CaSYT1的转录水平受疫霉侵染以及外源SA的诱导，且在疫霉侵染以及外源SA处理下CaSYT1启动子活性被激活并驱动了下游基因的转录表达；CaSYT1的瞬时表达能够诱发辣椒叶片内H_2O_2的大量积累，触发了明显的过敏性细胞坏死；CaSYT1沉默增强了辣椒应答疫霉侵染的抗病性，且伴随着CaPR1、NPR1、CaDEF1抗病相关基因的上调表达，暗示CaSYT1在辣椒应答疫霉侵染中起负调节作用，而其瞬时表达却能触发H_2O_2的积累和过敏性细胞坏死，表明CaSYT1以不依赖于细胞坏死和H_2O_2积累的方式在辣椒应答疫霉侵染中起着负调节作用。
Keyword ：
CaSYT1 CaSYT1 信号通路 信号通路 基因鉴定 基因鉴定 疫霉 疫霉 表达分析 表达分析 辣椒 辣椒
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| GB/T 7714 | 刘艳艳 , 丁颖 , 刘兴华 et al. 辣椒CaSYT1的鉴定及其在疫霉侵染过程中的功能初探 [J]. | 园艺学报 , 2024 , 51 (03) : 533-544 . |
| MLA | 刘艳艳 et al. "辣椒CaSYT1的鉴定及其在疫霉侵染过程中的功能初探" . | 园艺学报 51 . 03 (2024) : 533-544 . |
| APA | 刘艳艳 , 丁颖 , 刘兴华 , 郑佳秋 , 刘志钦 . 辣椒CaSYT1的鉴定及其在疫霉侵染过程中的功能初探 . | 园艺学报 , 2024 , 51 (03) , 533-544 . |
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As an important member of mitogen-activated protein kinase (MAPK) cascades, MAPKs play an important role in plant defense response against biotic and abiotic stresses; however, the involvement of the majority of the MAPK family members against Ralstonia solanacearum and heat stress (HS) remains poorly understood. In the present study, CaMAPK1 was identified from the genome of pepper and its function against R. solanacearum and HS was analyzed. The transcript accumulations of CaMAPK1 and the activities of its native promoter were both significantly induced by R. solanacearum inoculation, HS, and the application of exogenous hormones, including SA, MeJA, and ABA. Transient expression of CaMAPK1 showed that CaMAPK1 can be targeted throughout the whole cells in Nicotiana benthamiana and triggered chlorosis and hypersensitive response-like cell death in pepper leaves, accompanied by the accumulation of H2O2, and the up-regulations of hormones- and H2O2-associated marker genes. The knock-down of CaMAPK1 enhanced the susceptibility to R. solanacearum partially by down-regulating the expression of hormones- and H2O2-related genes and impairing the thermotolerance of pepper probably by attenuating CaHSFA2 and CaHSP70-1 transcripts. Taken together, our results revealed that CaMAPK1 is regulated by SA, JA, and ABA signaling and coordinates responses to R. solanacearum infection and HS in pepper.
Keyword ：
Capsicum annuum Capsicum annuum defense signaling defense signaling disease resistance disease resistance MAPK MAPK thermotolerance thermotolerance
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| GB/T 7714 | Shi, Lanping , Shi, Wei , Qiu, Zhengkun et al. CaMAPK1 Plays a Vital Role in the Regulation of Resistance to Ralstonia solanacearum Infection and Tolerance to Heat Stress [J]. | PLANTS-BASEL , 2024 , 13 (13) . |
| MLA | Shi, Lanping et al. "CaMAPK1 Plays a Vital Role in the Regulation of Resistance to Ralstonia solanacearum Infection and Tolerance to Heat Stress" . | PLANTS-BASEL 13 . 13 (2024) . |
| APA | Shi, Lanping , Shi, Wei , Qiu, Zhengkun , Yan, Shuangshuang , Liu, Zhiqin , Cao, Bihao . CaMAPK1 Plays a Vital Role in the Regulation of Resistance to Ralstonia solanacearum Infection and Tolerance to Heat Stress . | PLANTS-BASEL , 2024 , 13 (13) . |
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High temperature stress (HTS) is a serious threat to plant growth and development and to crop production in the context of global warming, and plant response to HTS is largely regulated at the transcriptional level by the actions of various transcription factors (TFs). However, whether and how homeodomain-leucine zipper (HD-Zip) TFs are involved in thermotolerance are unclear. Herein, we functionally characterized a pepper (Capsicum annuum) HD-Zip I TF CaHDZ15. CaHDZ15 expression was upregulated by HTS and abscisic acid in basal thermotolerance via loss- and gain-of-function assays by virus-induced gene silencing in pepper and overexpression in Nicotiana benthamiana plants. CaHDZ15 acted positively in pepper basal thermotolerance by directly targeting and activating HEAT SHOCK FACTORA6a (HSFA6a), which further activated CaHSFA2. In addition, CaHDZ15 interacted with HEAT SHOCK PROTEIN 70-2 (CaHsp70-2) and glyceraldehyde-3-phosphate dehydrogenase1 (CaGAPC1), both of which positively affected pepper thermotolerance. CaHsp70-2 and CaGAPC1 promoted CaHDZ15 binding to the promoter of CaHSFA6a, thus enhancing its transcription. Furthermore, CaHDZ15 and CaGAPC1 were protected from 26S proteasome-mediated degradation by CaHsp70-2 via physical interaction. These results collectively indicate that CaHDZ15, modulated by the interacting partners CaGAPC1 and CaHsp70-2, promotes basal thermotolerance by directly activating the transcript of CaHSFA6a. Thus, a molecular linkage is established among CaHsp70-2, CaGAPC1, and CaHDZ15 to transcriptionally modulate CaHSFA6a in pepper thermotolerance. The transcription factor CaHDZ15, modulated by cytoplasmic glyceraldehyde-3-phosphate dehydrogenase1 and heat shock protein 70-2, promotes pepper thermotolerance by activating HEAT SHOCK FACTORA6a.
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| GB/T 7714 | Mou, Shaoliang , He, Weihong , Jiang, Haitao et al. Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a [J]. | PLANT PHYSIOLOGY , 2024 , 195 (1) : 812-831 . |
| MLA | Mou, Shaoliang et al. "Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a" . | PLANT PHYSIOLOGY 195 . 1 (2024) : 812-831 . |
| APA | Mou, Shaoliang , He, Weihong , Jiang, Haitao , Meng, Qianqian , Zhang, Tingting , Liu, Zhiqin et al. Transcription factor CaHDZ15 promotes pepper basal thermotolerance by activating HEAT SHOCK FACTORA6a . | PLANT PHYSIOLOGY , 2024 , 195 (1) , 812-831 . |
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As important transcription factors, WRKYs play a vital role in the defense response of plants against the invasion of multiple pathogens. Though some WRKY members have been reported to participate in pepper immunity in response to Ralstonia solanacearum infection, the functions of the majority of WRKY members are still unknown. Herein, CaWRKY22b was cloned from the pepper genome and its function against R. solanacearum was analyzed. The transcript abundance of CaWRKY22b was significantly increased in response to the infection of R. solanacearum and the application of exogenous methyl jasmonate (MeJA). Subcellular localization assay in the leaves of Nicotiana benthamiana showed that CaWRKY22b protein was targeted to the nuclei. Agrobacterium-mediated transient expression in pepper leaves indicated that CaWRKY22b overexpression triggered intensive hypersensitive response-like cell death, H2O2 accumulation, and the up-regulation of defense- and JA-responsive genes, including CaHIR1, CaPO2, CaBPR1, and CaDEF1. Virus-induced gene silencing assay revealed that knock-down of CaWRKY22b attenuated pepper's resistance against R. solanacearum and the up-regulation of the tested defense- and jasmonic acid (JA)-responsive genes. We further assessed the role of CaWRKY22b in modulating the expression of JA-responsive CaDEF1, and the result demonstrated that CaWRKY22b trans-activated CaDEF1 expression by directly binding to its upstream promoter. Collectively, our results suggest that CaWRKY22b positively regulated pepper immunity against R. solanacearum in a manner associated with JA signaling, probably by modulating the expression of JA-responsive CaDEF1.
Keyword ：
immunity immunity JA signaling JA signaling pepper pepper Ralstonia solanacearum Ralstonia solanacearum WRKY transcription factor WRKY transcription factor
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| GB/T 7714 | Shi, Lanping , Fan, Yuemin , Yang, Yingjie et al. CaWRKY22b Plays a Positive Role in the Regulation of Pepper Resistance to Ralstonia solanacearum in a Manner Associated with Jasmonic Acid Signaling [J]. | PLANTS-BASEL , 2024 , 13 (15) . |
| MLA | Shi, Lanping et al. "CaWRKY22b Plays a Positive Role in the Regulation of Pepper Resistance to Ralstonia solanacearum in a Manner Associated with Jasmonic Acid Signaling" . | PLANTS-BASEL 13 . 15 (2024) . |
| APA | Shi, Lanping , Fan, Yuemin , Yang, Yingjie , Yan, Shuangshuang , Qiu, Zhengkun , Liu, Zhiqin et al. CaWRKY22b Plays a Positive Role in the Regulation of Pepper Resistance to Ralstonia solanacearum in a Manner Associated with Jasmonic Acid Signaling . | PLANTS-BASEL , 2024 , 13 (15) . |
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Plants have evolved a sophisticated immune system to defend against invasion by pathogens. In response, pathogens deploy copious effectors to evade the immune responses. However, the molecular mechanisms used by pathogen effectors to suppress plant immunity remain unclear. Herein, we report that an effector secreted by Ralstonia solanacearum, RipAK, modulates the transcriptional activity of the ethylene-responsive factor ERF098 to suppress immunity and dehydration tolerance, which causes bacterial wilt in pepper (Capsicum annuum L.) plants. Silencing ERF098 enhances the resistance of pepper plants to R. solanacearum infection not only by inhibiting the host colonization of R. solanacearum but also by increasing the immunity and tolerance of pepper plants to dehydration and including the closure of stomata to reduce the loss of water in an abscisic acid signal-dependent manner. In contrast, the ectopic expression of ERF098 in Nicotiana benthamiana enhances wilt disease. We also show that RipAK targets and inhibits the ERF098 homodimerization to repress the expression of salicylic acid-dependent PR1 and dehydration tolerance-related OSR1 and OSM1 by cis-elements in their promoters. Taken together, our study reveals a regulatory mechanism used by the R. solanacearum effector RipAK to increase virulence by specifically inhibiting the homodimerization of ERF098 and reprogramming the transcription of PR1, OSR1, and OSM1 to boost susceptibility and dehydration sensitivity. Thus, our study sheds light on a previously unidentified strategy by which a pathogen simultaneously suppresses plant immunity and tolerance to dehydration by secreting an effector to interfere with the activity of a transcription factor and manipulate plant transcriptional programs.
Keyword ：
dehydration dehydration effector effector immunity immunity pepper pepper Ralstonia solanacearum Ralstonia solanacearum transcription factor transcription factor
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| GB/T 7714 | Liu, Kaisheng , Shi, Lanping , Luo, Hongli et al. Ralstonia solanacearum effector RipAK suppresses homodimerization of the host transcription factor ERF098 to enhance susceptibility and the sensitivity of pepper plants to dehydration [J]. | PLANT JOURNAL , 2023 , 117 (1) : 121-144 . |
| MLA | Liu, Kaisheng et al. "Ralstonia solanacearum effector RipAK suppresses homodimerization of the host transcription factor ERF098 to enhance susceptibility and the sensitivity of pepper plants to dehydration" . | PLANT JOURNAL 117 . 1 (2023) : 121-144 . |
| APA | Liu, Kaisheng , Shi, Lanping , Luo, Hongli , Zhang, Kan , Liu, Jianxin , Qiu, Shanshan et al. Ralstonia solanacearum effector RipAK suppresses homodimerization of the host transcription factor ERF098 to enhance susceptibility and the sensitivity of pepper plants to dehydration . | PLANT JOURNAL , 2023 , 117 (1) , 121-144 . |
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Proteins with conserved SET domain play a critical role in plant immunity. However, the means of organization and functions of these proteins are unclear, particularly in non-model plants such as pepper (Capsicum annum L.). Herein, we functionally characterized CaASHH3, a member of class II (the ASH1 homologs H3K36) proteins in pepper immunity against Ralstonia solanacearum and Pseudomonas syringae pv tomato DC3000 (Pst DC3000). The CaASHH3 was localized in the nucleus, and its transcript levels were significantly enhanced by R. solanacearum inoculation (RSI) and exogenous application of salicylic acid (SA), methyl jasmonate (MeJA), ethephon (ETH), and abscisic acid (ABA). Knockdown of CaASHH3 by virus-induced gene silencing (VIGS) compromised peppers' resistance to RSI. Furthermore, silencing of CaASHH3 impaired hypersensitive-response (HR)-like cell death response due to RSI and downregulated defense-associated marker genes, including CaPR1, CaNPR1, and CaABR1. The CaASHH3 protein was revealed to affect the promoters of CaNPR1, CaPR1, and CaHSP24. Transiently over-expression of CaASHH3 in pepper leaves elicited HR-like cell death and upregulated immunity-related marker genes. To further study the role of CaASHH3 in plant defense in vivo, CaASHH3 transgenic plants were generated in Arabidopsis. Overexpression of CaASHH3 in transgenic Arabidopsis thaliana enhanced innate immunity against Pst DC3000. Furthermore, CaASHH3 over-expressing transgenic A. thaliana plants exhibited upregulated transcriptional levels of immunity-associated marker genes, such as AtNPR1, AtPR1, and AtPR2. These results collectively confirm the role of CaASHH3 as a positive regulator of plant cell death and pepper immunity against bacterial pathogens, which is regulated by signaling synergistically mediated by SA, JA, ET, and ABA.
Keyword ：
CaASHH3 CaASHH3 immunity immunity Pseudomonas syringae Pseudomonas syringae Ralstonia solanacearum Ralstonia solanacearum
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| GB/T 7714 | Hussain, Ansar , Liu Kaisheng , Noman, Ali et al. N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (12) . |
| MLA | Hussain, Ansar et al. "N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 . 12 (2022) . |
| APA | Hussain, Ansar , Liu Kaisheng , Noman, Ali , Ashraf, Muhammad Furqan , Albaqami, Mohammed , Khan, Muhammad Ifnan et al. N-Methyltransferase CaASHH3 Acts as a Positive Regulator of Immunity against Bacterial Pathogens in Pepper . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (12) . |
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Bacterial wilt, a severe disease involving vascular system blockade, is caused by Ralstonia solanacearum. Although both plant immunity and dehydration tolerance might contribute to disease resistance, whether and how they are related remains unclear. Herein, we showed that immunity against R. solanacearum and dehydration tolerance are coupled and regulated by the CaPti1-CaERF3 module. CaPti1 and CaERF3 are members of the serine/threonine protein kinase and ethylene-responsive factor families, respectively. Expression profiling revealed that CaPti1 and CaERF3 were upregulated by R. solanacearum inoculation, dehydration stress, and exogenously applied abscisic acid (ABA). They in turn phenocopied each other in promoting resistance of pepper (Capsicum annuum) to bacterial wilt not only by activating salicylic acid-dependent CaPR1, but also by activating dehydration tolerance-related CaOSM1 and CaOSR1 and inducing stomatal closure to reduce water loss in an ABA signaling-dependent manner. Our yeast two hybrid assay showed that CaERF3 interacted with CaPti1, which was confirmed using co-immunoprecipitation, bimolecular fluorescence complementation, and pull-down assays. Chromatin immunoprecipitation and electrophoretic mobility shift assays showed that upon R. solanacearum inoculation, CaPR1, CaOSM1, and CaOSR1 were directly targeted and positively regulated by CaERF3 and potentiated by CaPti1. Additionally, our data indicated that the CaPti1-CaERF3 complex might act downstream of ABA signaling, as exogenously applied ABA did not alter regulation of stomatal aperture by the CaPti1-CaERF3 module. Importantly, the CaPti1-CaERF3 module positively affected pepper growth and the response to dehydration stress. Collectively, the results suggested that immunity and dehydration tolerance are coupled and positively regulated by CaPti1-CaERF3 in pepper plants to enhance resistance against R. solanacearum.
Keyword ：
kinase kinase pepper pepper Ralstonia solanacearum Ralstonia solanacearum trans-activation trans-activation transcription factor transcription factor
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| GB/T 7714 | Shi, Lanping , Li, Xia , Weng, Yahong et al. The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance [J]. | PLANT JOURNAL , 2022 , 111 (1) : 250-268 . |
| MLA | Shi, Lanping et al. "The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance" . | PLANT JOURNAL 111 . 1 (2022) : 250-268 . |
| APA | Shi, Lanping , Li, Xia , Weng, Yahong , Cai, Hanyang , Liu, Kaisheng , Xie, Baixue et al. The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance . | PLANT JOURNAL , 2022 , 111 (1) , 250-268 . |
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【目的】探讨油柰（Prunus salicina lindley）WRKY33启动子在不同胁迫处理下的表达模式，为进一步深入研究油柰WRKY基因在油柰生长发育或抵御各种逆境胁迫中的作用机制等提供理论依据。【方法】利用MEGA6.06软件构建PsWRKY33的系统进化树。通过染色体步移技术克隆获得该基因启动子序列，利用PlantCARE数据库分析预测PsWRKY33 5′端上游启动子区域的顺式作用元件。利用拟南芥浸花法获得转基因植株。通过对不同胁迫处理下的各片段转基因幼苗进行组织化学染色和GUS酶活性测定。【结果】系统进化树分析表明PsWRKY蛋白与拟南芥WRKY33亲缘关系最近，故将该基因命名为PsWRKY33。获得PsWRKY33基因5′端上游启动子序列长度为1 872 bp，经预测分析发现该启动子区域含有ABRE、ARE、LTR、MYB和W-box等响应不同植物激素等顺式作用元件，基于此构建3个不同长度的缺失片段。对转基因植株不同组织GUS染色发现PsWRKY33启动子主要表达在叶、花瓣和花序梗上，且随着片段的缺失其表达越低。在不同胁迫处理下，其GUS活性表达程度不同。在低温胁迫条件下，不同长度的PsWRKY33启动子片段均受到不同程度的诱导上调表达；而在SA胁迫下，各片段呈现出不同程度的诱导下调表达。【结论】PsWRKY33基因启动子可能参与调节油柰应答低温胁迫及外源激素SA的响应。
Keyword ：
PsWRKY33 PsWRKY33 启动子 启动子 油柰 油柰 组织化学染色 组织化学染色 胁迫 胁迫
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| GB/T 7714 | 陈永萍 , 何水林 , 刘志钦 et al. 油柰PsWRKY33基因启动子的克隆与表达分析 [J]. | 福建农业学报 , 2022 , 37 (02) : 170-177 . |
| MLA | 陈永萍 et al. "油柰PsWRKY33基因启动子的克隆与表达分析" . | 福建农业学报 37 . 02 (2022) : 170-177 . |
| APA | 陈永萍 , 何水林 , 刘志钦 , 陈桂信 , 蒋际谋 . 油柰PsWRKY33基因启动子的克隆与表达分析 . | 福建农业学报 , 2022 , 37 (02) , 170-177 . |
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