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Characterization of Highbush Blueberry (Vaccinium corymbosum L.) Anthocyanin Biosynthesis Related MYBs and Functional Analysis of VcMYB Gene SCIE
期刊论文 | 2023 , 45 (1) , 379-399 | CURRENT ISSUES IN MOLECULAR BIOLOGY
WoS CC Cited Count: 6
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Abstract :

As one of the most important transcription factors regulating plant anthocyanin biosynthesis, MYB has attracted great attentions. In this study, we identified fifteen candidate anthocyanin biosynthesis related MYB (ABRM) proteins, including twelve R2R3-MYBs and three 1R-MYBs, from highbush blueberry. The subcellular localization prediction results showed that, with the exception of VcRVE8 (localized in chloroplast and nucleus), all of the blueberry ABRMs were nucleus-localized. The gene structure analysis revealed that the exon numbers of the blueberry ABRM genes varied greatly, ranging between one and eight. There are many light-responsive, phytohormone-responsive, abiotic stress-responsive and plant growth and development related cis-acting elements in the promoters of the blueberry ABRM genes. It is noteworthy that almost all of their promoters contain light-, ABA- and MeJA-responsive elements, which is consistent with the well-established results that anthocyanin accumulation and the expression of MYBs are influenced significantly by many factors, such as light, ABA and JA. The gene expression analysis revealed that VcMYB, VcMYB6, VcMYB23, VcMYBL2 and VcPH4 are expressed abundantly in blueberry fruits, and VcMYB is expressed the highest in the red, purple and blue fruits among all blueberry ABRMs. VcMYB shared high similarity with functionally proven ABRMs from many other plant species. The gene cloning results showed that VcMYB had three variable transcripts, but only the transient overexpression of VcMYB-1 promoted anthocyanin accumulation in the green fruits. Our study can provide a basis for future research on the anthocyanin biosynthesis related MYBs in blueberry.

Keyword :

anthocyanin anthocyanin blueberry blueberry expression regulation expression regulation gene expression gene expression MYB MYB

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GB/T 7714 Zhang, Yongyan , Huang, Dingquan , Wang, Bin et al. Characterization of Highbush Blueberry (Vaccinium corymbosum L.) Anthocyanin Biosynthesis Related MYBs and Functional Analysis of VcMYB Gene [J]. | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2023 , 45 (1) : 379-399 .
MLA Zhang, Yongyan et al. "Characterization of Highbush Blueberry (Vaccinium corymbosum L.) Anthocyanin Biosynthesis Related MYBs and Functional Analysis of VcMYB Gene" . | CURRENT ISSUES IN MOLECULAR BIOLOGY 45 . 1 (2023) : 379-399 .
APA Zhang, Yongyan , Huang, Dingquan , Wang, Bin , Yang, Xuelian , Wu, Huan , Qu, Pengyan et al. Characterization of Highbush Blueberry (Vaccinium corymbosum L.) Anthocyanin Biosynthesis Related MYBs and Functional Analysis of VcMYB Gene . | CURRENT ISSUES IN MOLECULAR BIOLOGY , 2023 , 45 (1) , 379-399 .
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The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis SCIE
期刊论文 | 2022 , 236 (4) , 1310-1325 | NEW PHYTOLOGIST
WoS CC Cited Count: 9
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Plant cells continuously experience mechanical stress resulting from the cell wall that bears internal turgor pressure. Cortical microtubules align with the predicted maximal tensile stress direction to guide cellulose biosynthesis and therefore results in cell wall reinforcement. We have previously identified Increased Petal Growth Anisotropy (IPGA1) as a putative microtubule-associated protein in Arabidopsis, but the function of IPGA1 remains unclear. Here, using the Arabidopsis cotyledon pavement cell as a model, we demonstrated that IPGA1 forms protein granules and interacts with ANGUSTIFOLIA (AN) to cooperatively regulate microtubule organisation in response to stress. Application of mechanical perturbations, such as cell ablation, led to microtubule reorganisation into aligned arrays in wild-type cells. This microtubule response to stress was enhanced in the IPGA1 loss-of-function mutant. Mechanical perturbations promoted the formation of IPGA1 granules on microtubules. We further showed that IPGA1 physically interacted with AN both in vitro and on microtubules. The ipga1 mutant alleles exhibited reduced interdigitated growth of pavement cells, with smooth shape. IPGA1 and AN had a genetic interaction in regulating pavement cell shape. Furthermore, IPGA1 genetically and physically interacted with the microtubule-severing enzyme KATANIN. We propose that the IPGA1-AN module regulates microtubule organisation and pavement cell shape.

Keyword :

Arabidopsis Arabidopsis cortical microtubule cortical microtubule mechanical stress mechanical stress microtubule organisation microtubule organisation pavement cells pavement cells

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GB/T 7714 Chen, Binqing , Dang, Xie , Bai, Wenting et al. The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis [J]. | NEW PHYTOLOGIST , 2022 , 236 (4) : 1310-1325 .
MLA Chen, Binqing et al. "The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis" . | NEW PHYTOLOGIST 236 . 4 (2022) : 1310-1325 .
APA Chen, Binqing , Dang, Xie , Bai, Wenting , Liu, Min , Li, Ying , Zhu, Lilan et al. The IPGA1-ANGUSTIFOLIA module regulates microtubule organisation and pavement cell shape in Arabidopsis . | NEW PHYTOLOGIST , 2022 , 236 (4) , 1310-1325 .
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大豆PIN-Like(PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能
期刊论文 | 2022 , 48 (02) , 353-366 | 作物学报
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植物激素生长素在植物的生长发育过程中发挥了至关重要的作用,它的稳态和浓度梯度建立控制了几乎所有器官的极性建成。生长素在特定细胞中合成、运输、感知以及代谢降解建立了符合器官发育的生长素浓度梯度。在豆科植物中,根与土壤微生物互作形成了根瘤这一特殊的器官,进行生物固氮。然而,生长素稳态控制生物固氮的功能还未知。拟南芥中的研究表明, PIN-Like (PILS)蛋白协助调节的细胞内生长素稳态,并介导下游细胞核内的生长素信号传递。本研究以大豆作为研究模型,在大豆基因组中鉴定获得19个PILS家族基因(GmPILS),不均匀分布于大豆10条染色体上。GmPILS在大豆9种组织部位中表现出多种表达模式,且具有明显的组织表达特异性。GmPILS1e和GmPILS1f在根瘤菌体区域富集表达,使用人工微RNA沉默(artificialmicro RNA interference,amiRNAi)下调GmPILS1e和GmPILS1f在根瘤的表达,导致根瘤的固氮酶活性上升,而过量表达GmPILS1f导致根瘤的固氮酶活性下降,因此GmPILS1e和GmPILS1f可能参与大豆固氮酶活性的调节。这些结果为进一步解析大豆GmPILS家族基因的功能和作用机制奠定了基础,同时也为结瘤固氮在农业育种中的应用提供了有价值的基因资源。

Keyword :

PIN-Like (PILS)基因家族 PIN-Like (PILS)基因家族 共生固氮 共生固氮 大豆 大豆 根瘤 根瘤

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GB/T 7714 董衍坤 , 黄定全 , 高震 et al. 大豆PIN-Like(PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能 [J]. | 作物学报 , 2022 , 48 (02) : 353-366 .
MLA 董衍坤 et al. "大豆PIN-Like(PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能" . | 作物学报 48 . 02 (2022) : 353-366 .
APA 董衍坤 , 黄定全 , 高震 , 陈栩 . 大豆PIN-Like(PILS)基因家族的鉴定、表达分析及在根瘤共生固氮过程中的功能 . | 作物学报 , 2022 , 48 (02) , 353-366 .
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GmPIN-dependent polar auxin transport is involved in soybean nodule development SCIE
期刊论文 | 2021 , 33 (9) , 2981-3003 | PLANT CELL
WoS CC Cited Count: 39
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To overcome nitrogen deficiency, legume roots establish symbiotic interactions with nitrogen-fixing rhizobia that are fostered in specialized organs (nodules). Similar to other organs, nodule formation is determined by a local maximum of the phytohormone auxin at the primordium site. However, how auxin regulates nodule development remains poorly understood. Here, we found that in soybean, (Glycine max), dynamic auxin transport driven by PIN-FORMED (PIN) transporter GmPIN1 is involved in nodule primordium formation. GmPIN1 was specifically expressed in nodule primordium cells and GmPIN1 was polarly localized in these cells. Two nodulation regulators, (iso)flavonoids trigger expanded distribution of GmPIN1b to root cortical cells, and cytokinin rearranges GmPIN1b polarity. Gmpin1abc triple mutants generated with CRISPR-Cas9 showed the impaired establishment of auxin maxima in nodule meristems and aberrant divisions in the nodule primordium cells. Moreover, overexpression of GmPIN1 suppressed nodule primordium initiation. GmPIN9d, an ortholog of Arabidopsis thaliana PIN2, acts together with GmPIN1 later in nodule development to acropetally transport auxin in vascular bundles, fine-tuning the auxin supply for nodule enlargement. Our findings reveal how PIN-dependent auxin transport modulates different aspects of soybean nodule development and suggest that the establishment of auxin gradient is a prerequisite for the proper interaction between legumes and rhizobia.

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GB/T 7714 Gao, Zhen , Chen, Zhiwei , Cui, Yuanyuan et al. GmPIN-dependent polar auxin transport is involved in soybean nodule development [J]. | PLANT CELL , 2021 , 33 (9) : 2981-3003 .
MLA Gao, Zhen et al. "GmPIN-dependent polar auxin transport is involved in soybean nodule development" . | PLANT CELL 33 . 9 (2021) : 2981-3003 .
APA Gao, Zhen , Chen, Zhiwei , Cui, Yuanyuan , Ke, Meiyu , Xu, Huifang , Xu, Qinzhen et al. GmPIN-dependent polar auxin transport is involved in soybean nodule development . | PLANT CELL , 2021 , 33 (9) , 2981-3003 .
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Salicylic acid regulatesPIN2auxin transporter hyperclustering and root gravitropic growth viaRemorin-dependent lipid nanodomain organisation inArabidopsis thaliana SCIE
期刊论文 | 2020 , 229 (2) , 963-978 | NEW PHYTOLOGIST
WoS CC Cited Count: 48
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To adapt to the diverse array of biotic and abiotic cues, plants have evolved sophisticated mechanisms to sense changes in environmental conditions and modulate their growth. Growth-promoting hormones and defence signalling fine tune plant development antagonistically. During host-pathogen interactions, this defence-growth trade-off is mediated by the counteractive effects of the defence hormone salicylic acid (SA) and the growth hormone auxin. Here we revealed an underlying mechanism of SA regulating auxin signalling by constraining the plasma membrane dynamics of PIN2 auxin efflux transporter inArabidopsis thalianaroots. The lateral diffusion of PIN2 proteins is constrained by SA signalling, during which PIN2 proteins are condensed into hyperclusters depending on REM1.2-mediated nanodomain compartmentalisation. Furthermore, membrane nanodomain compartmentalisation by SA or Remorin (REM) assembly significantly suppressed clathrin-mediated endocytosis. Consequently, SA-induced heterogeneous surface condensation disrupted asymmetric auxin distribution and the resultant gravitropic response. Our results demonstrated a defence-growth trade-off mechanism by which SA signalling crosstalked with auxin transport by concentrating membrane-resident PIN2 into heterogeneous compartments.

Keyword :

Arabidopsis Arabidopsis auxin transport auxin transport gravitropic growth gravitropic growth nanodomain nanodomain PIN2 hyperclustering PIN2 hyperclustering remorin remorin salicylic acid salicylic acid

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GB/T 7714 Ke, Meiyu , Ma, Zhiming , Wang, Deyan et al. Salicylic acid regulatesPIN2auxin transporter hyperclustering and root gravitropic growth viaRemorin-dependent lipid nanodomain organisation inArabidopsis thaliana [J]. | NEW PHYTOLOGIST , 2020 , 229 (2) : 963-978 .
MLA Ke, Meiyu et al. "Salicylic acid regulatesPIN2auxin transporter hyperclustering and root gravitropic growth viaRemorin-dependent lipid nanodomain organisation inArabidopsis thaliana" . | NEW PHYTOLOGIST 229 . 2 (2020) : 963-978 .
APA Ke, Meiyu , Ma, Zhiming , Wang, Deyan , Sun, Yanbiao , Wen, Chenjin , Huang, Dingquan et al. Salicylic acid regulatesPIN2auxin transporter hyperclustering and root gravitropic growth viaRemorin-dependent lipid nanodomain organisation inArabidopsis thaliana . | NEW PHYTOLOGIST , 2020 , 229 (2) , 963-978 .
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Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization (vol 116, pg 21274, 2019) SCIE
期刊论文 | 2020 , 117 (15) , 8659-8659 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
WoS CC Cited Count: 4
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GB/T 7714 Huang, Dingquan , Sun, Yanbiao , Ma, Zhiming et al. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization (vol 116, pg 21274, 2019) [J]. | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA , 2020 , 117 (15) : 8659-8659 .
MLA Huang, Dingquan et al. "Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization (vol 116, pg 21274, 2019)" . | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117 . 15 (2020) : 8659-8659 .
APA Huang, Dingquan , Sun, Yanbiao , Ma, Zhiming , Ke, Meiyu , Cui, Yong , Chen, Zichen et al. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization (vol 116, pg 21274, 2019) . | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA , 2020 , 117 (15) , 8659-8659 .
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Dynamic regulation of plasmodesmatal permeability and its application to horticultural research SCIE
期刊论文 | 2019 , 6 | HORTICULTURE RESEARCH
WoS CC Cited Count: 26
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Effective cell-to-cell communication allows plants to fine-tune their developmental processes in accordance with the prevailing environmental stimuli. Plasmodesmata (PD) are intercellular channels that span the plant cell wall and serve as cytoplasmic bridges to facilitate efficient exchange of signaling molecules between neighboring cells. The identification of PD-associated proteins and the subsequent elucidation of the regulation of PD structure have provided vital insights into the role of PD architecture in enforcing crucial cellular processes, including callose deposition, ER-Golgi-based secretion, cytoskeleton dynamics, membrane lipid raft organization, chloroplast metabolism, and cell wall formation. In this review, we summarize the emerging discoveries from recent studies that elucidated the regulatory mechanisms involved in PD biogenesis and the dynamics of PD opening-closure. Retrospectively, PD-mediated cell-to-cell communication has been implicated in diverse cellular and physiological processes that are fundamental for the development of horticultural plants. The potential application of PD biotechnological engineering represents a powerful approach for improving agronomic traits in horticultural crops in the future.

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GB/T 7714 Sun, Yanbiao , Huang, Dingquan , Chen, Xu . Dynamic regulation of plasmodesmatal permeability and its application to horticultural research [J]. | HORTICULTURE RESEARCH , 2019 , 6 .
MLA Sun, Yanbiao et al. "Dynamic regulation of plasmodesmatal permeability and its application to horticultural research" . | HORTICULTURE RESEARCH 6 (2019) .
APA Sun, Yanbiao , Huang, Dingquan , Chen, Xu . Dynamic regulation of plasmodesmatal permeability and its application to horticultural research . | HORTICULTURE RESEARCH , 2019 , 6 .
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Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization SCIE
期刊论文 | 2019 , 116 (42) , 21274-21284 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
WoS CC Cited Count: 107
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Plasmodesmata (PD) are plant-specific membrane-lined channels that create cytoplasmic and membrane continuities between adjacent cells, thereby facilitating cell-cell communication and virus movement. Plant cells have evolved diverse mechanisms to regulate PD plasticity in response to numerous environmental stimuli. In particular, during defense against plant pathogens, the defense hormone, salicylic acid (SA), plays a crucial role in the regulation of PD permeability in a callose-dependent manner. Here, we uncover a mechanism by which plants restrict the spreading of virus and PD cargoes using SA signaling by increasing lipid order and closure of PD. We showed that exogenous SA application triggered the compartmentalization of lipid raft nanodomains through a modulation of the lipid raft-regulatory protein, Remorin (REM). Genetic studies, superresolution imaging, and transmission electron microscopy observation together demonstrated that Arabidopsis REM1.2 and REM1.3 are crucial for plasma membrane nanodomain assembly to control PD aperture and functionality. In addition, we also found that a 14-3-3 epsilon protein modulates REM clustering and membrane nanodomain compartmentalization through its direct interaction with REM proteins. This study unveils a molecular mechanism by which the key plant defense hormone, SA, triggers membrane lipid nanodomain reorganization, thereby regulating PD closure to impede virus spreading.

Keyword :

membrane nanodomain compartmentalization membrane nanodomain compartmentalization plasmodesmata closure plasmodesmata closure Remorin Remorin SA-controlled lipid order SA-controlled lipid order

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GB/T 7714 Huang, Dingquan , Sun, Yanbiao , Ma, Zhiming et al. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization [J]. | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA , 2019 , 116 (42) : 21274-21284 .
MLA Huang, Dingquan et al. "Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization" . | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116 . 42 (2019) : 21274-21284 .
APA Huang, Dingquan , Sun, Yanbiao , Ma, Zhiming , Ke, Meiyu , Cui, Yong , Chen, Zichen et al. Salicylic acid-mediated plasmodesmal closure via Remorin-dependent lipid organization . | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA , 2019 , 116 (42) , 21274-21284 .
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水杨酸依赖Remorin调控脂质排布介导胞间连丝关闭
会议论文 | 2018 , 208 | 2018全国植物生物学大会
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<正>胞间连丝是脂阀富集的特化超微细胞结构,也是病毒入侵的一条主要途径。病原菌与宿主细胞互作,通过增加胞间连丝孔径,完成入侵扩散;而宿主则合成高水平的水杨酸,启动系统性防御反应。虽然水杨酸介导的下游信号已被阐明的相对清晰,然而胞间连丝的结构和功能如何参与水杨酸信号介导的植物免疫尚不清楚。本课题初步证明高水平水杨酸能够刺激细胞膜甾醇及脂阀特异定位的Remorin蛋白,使脂筏重新排布,同时关闭胞间连丝。冷冻透射电镜结合三维断层显像技术证明Remorin1.2,Remorin1.3突变体显著增加胞间连丝孔径而

Keyword :

Remorin Remorin 水杨酸 水杨酸 胞间连丝 胞间连丝

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GB/T 7714 黄定全 , 孙延彪 , 陈栩 . 水杨酸依赖Remorin调控脂质排布介导胞间连丝关闭 [C] //2018全国植物生物学大会论文集 . 2018 : 208 .
MLA 黄定全 et al. "水杨酸依赖Remorin调控脂质排布介导胞间连丝关闭" 2018全国植物生物学大会论文集 . (2018) : 208 .
APA 黄定全 , 孙延彪 , 陈栩 . 水杨酸依赖Remorin调控脂质排布介导胞间连丝关闭 2018全国植物生物学大会论文集 . (2018) : 208 .
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SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape SCIE
期刊论文 | 2016 , 172 (1) , 358-371 | PLANT PHYSIOLOGY
WoS CC Cited Count: 38
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Plant organ growth and final shape rely on cell proliferation and, particularly, on cell expansion that largely determines the visible growth of plant organs. Arabidopsis (Arabidopsis thaliana) petals serve as an excellent model for dissecting the coordinated regulation of patterns of cell expansion and organ growth, but the molecular signaling mechanisms underlying this regulation remain largely unknown. Here, we demonstrate that during the late petal development stages, SPIKE1 (SPK1), encoding a guanine nucleotide exchange factor, activates Rho of Plants (ROP) GTPase proteins (ROP2, ROP4, and ROP6) to affect anisotropic expansion of epidermal cells in both petal blades and claws, thereby affecting anisotropic growth of the petal and the final characteristic organ shape. The petals of SPK1 knockdown mutants were significantly longer but narrower than those of the wild type, associated with increased anisotropic expansion of epidermal cells at late development stages. In addition, ROP2, ROP4, and ROP6 are activated by SPK1 to promote the isotropic organization of cortical microtubule arrays and thus inhibit anisotropic growth in the petal. Both knockdown of SPK1 and multiple rop mutants caused highly ordered cortical microtubule arrays that were transversely oriented relative to the axis of cell elongation after development stage 11. Taken together, our results suggest a SPK1-ROP-dependent signaling module that influences anisotropic growth in the petal and defines the final organ shape.

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GB/T 7714 Ren, Huibo , Dang, Xie , Yang, Yanqiu et al. SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape [J]. | PLANT PHYSIOLOGY , 2016 , 172 (1) : 358-371 .
MLA Ren, Huibo et al. "SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape" . | PLANT PHYSIOLOGY 172 . 1 (2016) : 358-371 .
APA Ren, Huibo , Dang, Xie , Yang, Yanqiu , Huang, Dingquan , Liu, Mengting , Gao, Xiaowei et al. SPIKE1 Activates ROP GTPase to Modulate Petal Growth and Shape . | PLANT PHYSIOLOGY , 2016 , 172 (1) , 358-371 .
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