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学者姓名：官德义
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Abstract ：
CaWRKY40 coordinately activates pepper immunity against Ralstonia solanacearum infection (RSI) and high temperature stress (HTS), forms positive feedback loops with other positive regulators and is promoted by CaWRKY27b/CaWRKY28 through physical interactions; however, whether and how it is regulated by negative regulators to function appropriately remain unclear. Herein, we provide evidence that CaWRKY40 is repressed by a SALT TOLERANCE HOMOLOG2 in pepper (CaSTH2). Our data from gene silencing and transient overexpression in pepper and epoptic overexpression in Nicotiana benthamiana plants showed that CaSTH2 acted as negative regulator in immunity against RSI and thermotolerance. Our data from BiFC, CoIP, pull down, and MST indicate that CaSTH2 interacted with CaWRKY40, by which CaWRKY40 was prevented from activating immunity or thermotolerance-related genes. It was also found that CaSTH2 repressed CaWRKY40 at least partially through blocking interaction of CaWRKY40 with CaWRKY27b/CaWRKY28, but not through directly repressing binding of CaWRKY40 to its target genes. The results of study provide new insight into the mechanisms underlying the coordination of pepper immunity and thermotolerance.
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| GB/T 7714 | Cheng, Xingge , Wan, Meiyun , Song, Yuqiu et al. CaSTH2 disables CaWRKY40 from activating pepper thermotolerance and immunity against Ralstonia solanacearum via physical interaction [J]. | HORTICULTURE RESEARCH , 2024 , 11 (5) . |
| MLA | Cheng, Xingge et al. "CaSTH2 disables CaWRKY40 from activating pepper thermotolerance and immunity against Ralstonia solanacearum via physical interaction" . | HORTICULTURE RESEARCH 11 . 5 (2024) . |
| APA | Cheng, Xingge , Wan, Meiyun , Song, Yuqiu , Liu, Qian , Hu, Xiaohui , Chen, Xiufang et al. CaSTH2 disables CaWRKY40 from activating pepper thermotolerance and immunity against Ralstonia solanacearum via physical interaction . | HORTICULTURE RESEARCH , 2024 , 11 (5) . |
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[目的]青枯病是辣椒生产中主要的土传病害，在高温高湿下发病尤为严重。研究CaRPT2在高温高湿下辣椒应答青枯菌侵染过程中的作用，为开展辣椒抗青枯病遗传改良提供依据。[方法]以中抗青枯病辣椒自交系植株(HN42)与本氏烟草为试验材料，从辣椒叶片cDNA文库中克隆出NRL蛋白家族成员CaRPT2,对其进行同源比对和进化树分析，并通过瞬时过表达、亚细胞定位以及荧光定量PCR等技术研究CaRPT2在高温高湿下辣椒应答青枯菌侵染过程中的作用。[结果]CaRPT2的氨基酸序列与其他物种中的直系同源基因有着较高的同源性，CaRPT2蛋白定位在本氏烟草叶片的细胞质与细胞膜中，CaRPT2转录水平受高温高湿条件下青枯菌侵染的诱导表达，且在辣椒叶片中瞬时过表达CaRPT2,可显著诱导高温高湿下特异性抗病基因CaMgst3的转录激活。[结论]CaRPT2在高温高湿下辣椒应答青枯菌侵染过程中起正调节作用，可为进一步阐明RPT2基因在植物抗病中的功能提供参考。
Keyword ：
CaRPT2 CaRPT2 辣椒 辣椒 青枯菌 青枯菌 高温高湿 高温高湿
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| GB/T 7714 | 卢巧玲 , 万美云 , 严志彬 et al. 高温高湿下CaRPT2在辣椒抗青枯病中的作用 [J]. | 亚热带农业研究 , 2023 , 19 (03) : 145-152 . |
| MLA | 卢巧玲 et al. "高温高湿下CaRPT2在辣椒抗青枯病中的作用" . | 亚热带农业研究 19 . 03 (2023) : 145-152 . |
| APA | 卢巧玲 , 万美云 , 严志彬 , 官德义 , 何水林 . 高温高湿下CaRPT2在辣椒抗青枯病中的作用 . | 亚热带农业研究 , 2023 , 19 (03) , 145-152 . |
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Plant diseases tend to be more serious under conditions of high-temperature/high-humidity (HTHH) than under moderate conditions, and hence disease resistance under HTHH is an important determinant for plant survival. However, how plants cope with diseases under HTHH remains poorly understood. In this study, we used the pathosystem consisting of pepper (Capsicum annuum) and Ralstonia solanacearum (bacterial wilt) as a model to examine the functions of the protein mildew resistance locus O 1 (CaMLO1) and U-box domain-containing protein 21 (CaPUB21) under conditions of 80% humidity and either 28 degrees C or 37 degrees C. Expression profiling, loss- and gain-of-function assays involving virus-induced gene-silencing and overexpression in pepper plants, and protein-protein interaction assays were conducted, and the results showed that CaMLO1 acted negatively in pepper immunity against R. solanacearum at 28 degrees C but positively at 37 degrees C. In contrast, CaPUB21 acted positively in immunity at 28 degrees C but negatively at 37 degrees C. Importantly, CaPUB21 interacted with CaMLO1 under all of the tested conditions, but only the interaction in response to R. solanacearum at 37 degrees C or to exposure to 37 degrees C alone led to CaMLO1 degradation, thereby turning off defence responses against R. solanacearum at 37 degrees C and under high-temperature stress to conserve resources. Thus, we show that CaMLO1 and CaPUB21 interact with each other and function distinctly in pepper immunity against R. solanacearum in an environment-dependent manner. Mildew resistance locus O 1 in pepper interacts with U-box domain-containing protein 21 to provide defence against mildew that is specific to high temperatures, and also confers thermotolerance.
Keyword ：
Capsicum annuum Capsicum annuum defence response defence response high-temperature/high humidity high-temperature/high humidity mildew mildew MLO MLO pathogen resistance pathogen resistance pepper pepper plant imuunity plant imuunity plant U-box E3 ubiquitin ligase plant U-box E3 ubiquitin ligase Ralstonia solanacearum Ralstonia solanacearum wilt wilt
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| GB/T 7714 | Huang, Xueying , Yang, Sheng , Zhang, Yapeng et al. Temperature-dependent action of pepper mildew resistance locus O 1 in inducing pathogen immunity and thermotolerance [J]. | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 75 (7) : 2064-2083 . |
| MLA | Huang, Xueying et al. "Temperature-dependent action of pepper mildew resistance locus O 1 in inducing pathogen immunity and thermotolerance" . | JOURNAL OF EXPERIMENTAL BOTANY 75 . 7 (2023) : 2064-2083 . |
| APA | Huang, Xueying , Yang, Sheng , Zhang, Yapeng , Shi, Yuanyuan , Shen, Lei , Zhang, Qixiong et al. Temperature-dependent action of pepper mildew resistance locus O 1 in inducing pathogen immunity and thermotolerance . | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 75 (7) , 2064-2083 . |
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High temperature stress (HTS), with growth and development impairment, is one of the most important abiotic stresses frequently encountered by plants, in particular solanacaes such as pepper, that mainly distribute in tropical and subtropical regions. Plants activate thermotolerance to cope with this stress; however, the underlying mechanism is currently not fully understood. SWC4, a shared component of SWR1- and NuA4 complexes implicated in chromatin remodeling, was previously found to be involved in the regulation of pepper thermotolerance, but the underlying mechanism remains poorly understood. Herein, PMT6, a putative methyltranferase was originally found to interact with SWC4 by co-immunoprecipitation (Co-IP)-combined LC/MS assay. This interaction was further confirmed by bimolecular fluorescent complimentary (BiFC) and Co-IP assay, and PMT6 was further found to confer SWC4 methylation. By virus-induced gene silencing, it was found that PMT6 silencing significantly reduced pepper basal thermotolerance and transcription of CaHSP24 and significantly reduced the enrichment of chromatin-activation-related H3K9ac, H4K5ac, and H3K4me3 in TSS of CaHSP24, which was previously found to be positively regulated by CaSWC4. By contrast, the overexpression of PMT6 significantly enhanced basal thermotolerance of pepper plants. All these data indicate that PMT6 acts as a positive regulator in pepper thermotolerance, likely by methylating SWC4.
Keyword ：
Capsicum annuum Capsicum annuum heat stress heat stress PMT6 PMT6 SWC4 SWC4 thermotolerance thermotolerance
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| GB/T 7714 | Huang, Yu , Cai, Weiwei , Lu, Qiaoling et al. PMT6 Is Required for SWC4 in Positively Modulating Pepper Thermotolerance [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (5) . |
| MLA | Huang, Yu et al. "PMT6 Is Required for SWC4 in Positively Modulating Pepper Thermotolerance" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 5 (2023) . |
| APA | Huang, Yu , Cai, Weiwei , Lu, Qiaoling , Lv, Jingang , Wan, Meiyun , Guan, Deyi et al. PMT6 Is Required for SWC4 in Positively Modulating Pepper Thermotolerance . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (5) . |
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The authors reveal a mechanism in understanding the responses to Ralstonia solanacearum in pepper under high temperature and high humidity conditions, that is differential associations between transcription factors mediate distinct immune and heat responses. High temperature and high humidity (HTHH) conditions increase plant susceptibility to a variety of diseases, including bacterial wilt in solanaceous plants. Some solanaceous plant cultivars have evolved mechanisms to activate HTHH-specific immunity to cope with bacterial wilt disease. However, the underlying mechanisms remain poorly understood. Here we find that CaKAN3 and CaHSF8 upregulate and physically interact with each other in nuclei under HTHH conditions without inoculation or early after inoculation with R. solanacearum in pepper. Consequently, CaKAN3 and CaHSF8 synergistically confer immunity against R. solanacearum via activating a subset of NLRs which initiates immune signaling upon perception of unidentified pathogen effectors. Intriguingly, when HTHH conditions are prolonged without pathogen attack or the temperature goes higher, CaHSF8 no longer interacts with CaKAN3. Instead, it directly upregulates a subset of HSP genes thus activating thermotolerance. Our findings highlight mechanisms controlling context-specific activation of high-temperature-specific pepper immunity and thermotolerance mediated by differential CaKAN3-CaHSF8 associations.
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| GB/T 7714 | Yang, Sheng , Cai, Weiwei , Wu, Ruijie et al. Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions [J]. | NATURE COMMUNICATIONS , 2023 , 14 (1) . |
| MLA | Yang, Sheng et al. "Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions" . | NATURE COMMUNICATIONS 14 . 1 (2023) . |
| APA | Yang, Sheng , Cai, Weiwei , Wu, Ruijie , Huang, Yu , Lu, Qiaoling , Huang, Xueying et al. Differential CaKAN3-CaHSF8 associations underlie distinct immune and heat responses under high temperature and high humidity conditions . | NATURE COMMUNICATIONS , 2023 , 14 (1) . |
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Pepper (Capsicum annuum) employs distinct defence responses against Ralstonia solanacearum infection (RSI); however, the mechanisms by which pepper activates these defence responses in a context-dependent manner is unclear. Here we study pepper plants defence response to RSI under room temperature-high humidity (RSRT, 28 degrees C / 90%) and high temperature-high humidity (RSHT, 37 degrees C / 90%) conditions, and non-infected plants under high temperature-high humidity (HTHH, 42 degrees C / 90%) stress. Herein, we found that the MADS-box transcription factor CaAGL8 was up-regulated by HTHH stress and RSRT or RSHT, and its silencing significantly reduced pepper thermotolerance and susceptibility to infection under both room and high temperature-high humidity (RSRT and RSHT). This was coupled with down-regulation of CaSTH2 and CaDEF1 upon RSRT, down-regulation of CaMgst3 and CaPRP1 upon RSHT, and down-regulation of CaHSP24 upon HTHH. In contrast, the ectopic overexpression of CaAGL8 significantly increased the resistance of Nicotiana benthamiana plants to RSRT, RSHT, and HTHH. In addition, CaAGL8 was found to interact with CaSWC4, which acted as a positive regulator of the pepper response to RSRT, RSHT, and HTHH. Silencing of either CaAGL8 or CaSWC4 blocked the hypersensitive response (HR) cell death and context-dependent up-regulation of defence-related genes triggered by the other. Importantly, enrichment of H4K5Ac, H3K9Ac, H3K4me3, and H3K9me2 on the tested defence-related genes was context- and gene-specifically regulated through synergistic interaction between CaSWC4 and CaAGL8. Our results indicate that pepper employs CaAGL8 to modulate chromatin remodelling by interacting with CaSWC4, thereby activating defence responses to RSRT, RSHT, and HTHH.
Keyword ：
AGL8 AGL8 Capsicum annuum Capsicum annuum MADS MADS Ralstonia solanacearum Ralstonia solanacearum SWC4 SWC4 thermotolerance thermotolerance
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| GB/T 7714 | Zhang, Yapeng , Cai, Weiwei , Wang, Aiwen et al. MADS-box protein AGL8 interacts with chromatin-remodelling component SWC4 to activate thermotolerance and environment-dependent immunity in pepper [J]. | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 74 (12) : 3667-3683 . |
| MLA | Zhang, Yapeng et al. "MADS-box protein AGL8 interacts with chromatin-remodelling component SWC4 to activate thermotolerance and environment-dependent immunity in pepper" . | JOURNAL OF EXPERIMENTAL BOTANY 74 . 12 (2023) : 3667-3683 . |
| APA | Zhang, Yapeng , Cai, Weiwei , Wang, Aiwen , Huang, Xueying , Zheng, Xiang , Liu, Qian et al. MADS-box protein AGL8 interacts with chromatin-remodelling component SWC4 to activate thermotolerance and environment-dependent immunity in pepper . | JOURNAL OF EXPERIMENTAL BOTANY , 2023 , 74 (12) , 3667-3683 . |
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Pepper (Capsicum annuum) responds differently to high temperature stress (HTS) and Ralstonia solanacearum infection (RSI) but employs some shared transcription factors (TFs), such as CabZIP63 and CaWRKY40, in both cases. How the plant activates and balances these distinct responses, however, was unclear. Here, we show that the protein CaSWC4 interacts with CaRUVBL2 and CaTAF14b and they all act positively in pepper response to RSI and thermotolerance. CaSWC4 activates chromatin of immunity or thermotolerance related target genes of CaWRKY40 or CabZIP63 by promoting deposition of H2A.Z, H3K9ac and H4K5ac, simultaneously recruits CabZIP63 and CaWRKY40 through physical interaction and brings them to their targets (immunity- or thermotolerance-related genes) via binding AT-rich DNA element. The above process relies on the recruitment of CaRUVBL2 and TAF14 by CaSWC4 via physical interaction, which occurs at loci of immunity related target genes only when the plants are challenged with RSI, and at loci of thermotolerance related target genes only upon HTS. Collectively, our data suggest that CaSWC4 regulates rapid, accurate responses to both RSI and HTS by modulating chromatin of specific target genes opening and recruiting the TFs, CaRUVBL2 and CaTAF14b to the specific target genes, thereby helping achieve the balance between immunity and thermotolerance.
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| GB/T 7714 | Cai, Weiwei , Yang, Sheng , Wu, Ruijie et al. CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper [J]. | PLOS GENETICS , 2022 , 18 (2) . |
| MLA | Cai, Weiwei et al. "CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper" . | PLOS GENETICS 18 . 2 (2022) . |
| APA | Cai, Weiwei , Yang, Sheng , Wu, Ruijie , Zheng, Yutong , He, Shicong , Shen, Lei et al. CaSWC4 regulates the immunity-thermotolerance tradeoff by recruiting CabZIP63/CaWRKY40 to target genes and activating chromatin in pepper . | PLOS GENETICS , 2022 , 18 (2) . |
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| GB/T 7714 | Zheng, Yutong , He, Shicong , Cai, Weiwei et al. CaAIL1 Acts Positively in Pepper Immunity against Ralstonia solanacearum by Repressing Negative Regulators (vol 62, pg 702, 2021) [J]. | PLANT AND CELL PHYSIOLOGY , 2022 , 63 (1) : 149-149 . |
| MLA | Zheng, Yutong et al. "CaAIL1 Acts Positively in Pepper Immunity against Ralstonia solanacearum by Repressing Negative Regulators (vol 62, pg 702, 2021)" . | PLANT AND CELL PHYSIOLOGY 63 . 1 (2022) : 149-149 . |
| APA | Zheng, Yutong , He, Shicong , Cai, Weiwei , Shen, Lei , Huang, Xueying , Yang, Sheng et al. CaAIL1 Acts Positively in Pepper Immunity against Ralstonia solanacearum by Repressing Negative Regulators (vol 62, pg 702, 2021) . | PLANT AND CELL PHYSIOLOGY , 2022 , 63 (1) , 149-149 . |
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CabZIP63 and CaWRKY40 were previously found to be shared in the pepper defense response to high temperature stress (HTS) and to Ralstonia solanacearum inoculation (RSI), forming a transcriptional cascade. However, how they activate the two distinct defense responses is not fully understood. Herein, using a revised genetic approach, we functionally characterized CabZIP23 in the CabZIP63-CaWRKY40 cascade and its context specific pepper immunity activation against RSI by interaction with CabZIP63. CabZIP23 was originally found by immunoprecipitation-mass spectrometry to be an interacting protein of CabZIP63-GFP; it was upregulated by RSI and acted positively in pepper immunity against RSI by virus induced gene silencing in pepper plants, and transient overexpression in Nicotiana benthamiana plants. By chromatin immunoprecipitation (ChIP)-qPCR and electrophoresis mobility shift assay (EMSA), CabZIP23 was found to be directly regulated by CaWRKY40, and CabZIP63 was directly regulated by CabZIP23, forming a positive feedback loop. CabZIP23-CabZIP63 interaction was confirmed by co-immunoprecipitation (CoIP) and bimolecular fluorescent complimentary (BiFC) assays, which promoted CabZIP63 binding immunity related target genes, including CaPR1, CaNPR1 and CaWRKY40, thereby enhancing pepper immunity against RSI, but not affecting the expression of thermotolerance related CaHSP24. All these data appear to show that CabZIP23 integrates in the CabZIP63-CaWRKY40 cascade and the context specifically turns it on mounting pepper immunity against RSI.
Keyword ：
CabZIP23 CabZIP23 CabZIP63 CabZIP63 Capsicum annuum Capsicum annuum immunity immunity Ralstonia solanacearum Ralstonia solanacearum
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| GB/T 7714 | Lu, Qiaoling , Huang, Yu , Wang, Hui et al. CabZIP23 Integrates in CabZIP63-CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (5) . |
| MLA | Lu, Qiaoling et al. "CabZIP23 Integrates in CabZIP63-CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 23 . 5 (2022) . |
| APA | Lu, Qiaoling , Huang, Yu , Wang, Hui , Wan, Meiyun , Lv, Jingang , Cheng, Xingge et al. CabZIP23 Integrates in CabZIP63-CaWRKY40 Cascade and Turns CabZIP63 on Mounting Pepper Immunity against Ralstonia solanacearum via Physical Interaction . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2022 , 23 (5) . |
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Bacterial wilt, a severe disease involving vascular system blockade, is caused by Ralstonia solanacearum. Although both plant immunity and dehydration tolerance might contribute to disease resistance, whether and how they are related remains unclear. Herein, we showed that immunity against R. solanacearum and dehydration tolerance are coupled and regulated by the CaPti1-CaERF3 module. CaPti1 and CaERF3 are members of the serine/threonine protein kinase and ethylene-responsive factor families, respectively. Expression profiling revealed that CaPti1 and CaERF3 were upregulated by R. solanacearum inoculation, dehydration stress, and exogenously applied abscisic acid (ABA). They in turn phenocopied each other in promoting resistance of pepper (Capsicum annuum) to bacterial wilt not only by activating salicylic acid-dependent CaPR1, but also by activating dehydration tolerance-related CaOSM1 and CaOSR1 and inducing stomatal closure to reduce water loss in an ABA signaling-dependent manner. Our yeast two hybrid assay showed that CaERF3 interacted with CaPti1, which was confirmed using co-immunoprecipitation, bimolecular fluorescence complementation, and pull-down assays. Chromatin immunoprecipitation and electrophoretic mobility shift assays showed that upon R. solanacearum inoculation, CaPR1, CaOSM1, and CaOSR1 were directly targeted and positively regulated by CaERF3 and potentiated by CaPti1. Additionally, our data indicated that the CaPti1-CaERF3 complex might act downstream of ABA signaling, as exogenously applied ABA did not alter regulation of stomatal aperture by the CaPti1-CaERF3 module. Importantly, the CaPti1-CaERF3 module positively affected pepper growth and the response to dehydration stress. Collectively, the results suggested that immunity and dehydration tolerance are coupled and positively regulated by CaPti1-CaERF3 in pepper plants to enhance resistance against R. solanacearum.
Keyword ：
kinase kinase pepper pepper Ralstonia solanacearum Ralstonia solanacearum trans-activation trans-activation transcription factor transcription factor
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| GB/T 7714 | Shi, Lanping , Li, Xia , Weng, Yahong et al. The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance [J]. | PLANT JOURNAL , 2022 , 111 (1) : 250-268 . |
| MLA | Shi, Lanping et al. "The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance" . | PLANT JOURNAL 111 . 1 (2022) : 250-268 . |
| APA | Shi, Lanping , Li, Xia , Weng, Yahong , Cai, Hanyang , Liu, Kaisheng , Xie, Baixue et al. The CaPti1-CaERF3 module positively regulates resistance of Capsicum annuum to bacterial wilt disease by coupling enhanced immunity and dehydration tolerance . | PLANT JOURNAL , 2022 , 111 (1) , 250-268 . |
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