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Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress SCIE
期刊论文 | 2025 , 14 (5) | PLANTS-BASEL
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The tea plant (Camellia sinensis) is an economically important crop that plays an important role not only in the beverage industry but also in the pharmaceutical industry. The environment has a great influence on the quality of the tea plant. Heat shock factors (Hsfs) are transcriptional regulators that control the plant response to adversity. However, only a limited number of studies have reported the Hsf gene in Camellia sinensis, and most of these reports involve high-temperature, drought, and salt stress. Research on light, dark, and cold stress is limited. In this study, 22 CsHsf genes were obtained by whole genome sequencing and found to be located on 11 chromosomes. In addition, the gene structure, protein motif, and phylogeny were studied. We classified the genes into three major subfamilies: CsHsfA, CsHsfB, and CsHsfC. Interestingly, we found that there was more alignment between CsHsf and Hsf genes in dicotyledons, including Arabidopsis thaliana and Solanum lycopersicum, than in the monocotyledon Oryza sativa. The expression of many CsHsf genes was affected by low-temperature, light, and dark abiotic stresses. Notably, CsHsf15 and CsHsf16 showed high induction rates under both light and cold stress, and both genes carried cis-acting elements associated with light and low-temperature responses. These results lay a solid groundwork for further investigations into the involvement of CsHsf genes in the response of Camellia sinensis to abiotic stresses.

Keyword :

abiotic stress abiotic stress gene family gene family Hsf Hsf tea tea

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GB/T 7714 Li, Guimin , Shi, Xinying , Lin, Qinmin et al. Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress [J]. | PLANTS-BASEL , 2025 , 14 (5) .
MLA Li, Guimin et al. "Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress" . | PLANTS-BASEL 14 . 5 (2025) .
APA Li, Guimin , Shi, Xinying , Lin, Qinmin , Lv, Mengmeng , Chen, Jing , Wen, Yingxin et al. Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factors in Camellia sinensis Under Abiotic Stress . | PLANTS-BASEL , 2025 , 14 (5) .
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Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca SCIE
期刊论文 | 2025 , 14 (8) | BIOLOGY-BASEL
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Nitrogen (N) is crucial for plant growth and stress resistance and is primarily absorbed and transported by nitrate transporters (NRT). Suaeda glauca, known for its strong salt-alkali stress resistance, and SgNRT genes have rarely been reported. This study aims to identify and analyze the SgNRT gene family to understand its composition, evolutionary patterns, and roles in salt stress responses. We identified 212 SgNRTs, which were categorized into three branches, with SgNRT1/SgNPF and SgNRT2 as the major families. Structural analysis, conserved domains, chromosomal localization, and collinearity were also examined. Spatiotemporal expression characteristics of SgNRT genes were analyzed, revealing specific expression across 13 organs or tissues and dynamic responses to salt treatment over 48 h. Notably, SgNRT1.185, SgNRT2.25, and SgNRT2.2 exhibited rapid salt induction in leaves (activated within 0.5 h, peaking at 2 h), with SgNRT1.185 showing relatively high upregulation. SgNRT1.185 and SgNRT2.35 were induced by high salt concentrations (200 mM) in both roots and leaves. SgNRT2.35 exhibited higher basal and stress-induced levels than the other genes. Bioinformatics analysis suggests spatially specific expression of SgNRT genes, potentially involved in nitrogen absorption and transport across various developmental stages and organs/tissues of Suaeda glauca. These findings offer a theoretical basis for understanding the adaptive strategies of Suaeda glauca under saline-alkali stress and provide insights into the functional evolution of plant NRT genes, aiding in the development of stress-resistant crops.

Keyword :

bioinformatics bioinformatics gene expression analysis gene expression analysis NRT gene family NRT gene family soil salinization soil salinization Suaeda glauca Suaeda glauca

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GB/T 7714 Ou, Zitong , Sun, Jin , Li, Xueli et al. Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca [J]. | BIOLOGY-BASEL , 2025 , 14 (8) .
MLA Ou, Zitong et al. "Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca" . | BIOLOGY-BASEL 14 . 8 (2025) .
APA Ou, Zitong , Sun, Jin , Li, Xueli , Feng, Haoran , Chen, Xingguang , Liang, Sisi et al. Genome-Wide Identification, Characterization, and Expression Analysis of NRT Gene Family in Suaeda glauca . | BIOLOGY-BASEL , 2025 , 14 (8) .
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Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution SCIE
期刊论文 | 2025 , 177 (5) | PHYSIOLOGIA PLANTARUM
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Abstract :

The cell cycle is a fundamental process of plant growth, development, and reproduction, in which cyclin-dependent kinases (CDKs) and cyclins (CYCs) play central roles in regulating the progression through various stages. These proteins are coordinated with multiple interacting partners to ensure the accurate execution of essential biological events such as DNA replication, chromosome segregation, and cell division. Marchantia polymorpha, one of the earliest diverging land plant species, has emerged as a key model for exploring fundamental mechanisms in plant biology and evolution. However, compared with other model plants, such as Arabidopsis thaliana and Oryza sativa, the core cell cycle genes in M. polymorpha remain relatively uncharacterized. In this study, we identified 31 core cell cycle genes in M. polymorpha through genome-wide analysis, including 13 CDKs, 8 CYCs, 5 E2F/DPs, 1 ICK, 1 RB, 1 CKS, and 2 Wee1 genes. We further analyzed their physicochemical properties, gene structures, and conserved domains, along with evolutionary pressures assessed via Ka/Ks and 4DTv analyses. Comparative genomic analysis revealed patterns of gene contraction and expansion. Additionally, we predicted cis-acting regulatory elements and performed differential expression analysis under various stress conditions to explore their potential functions and expression profiles. Finally, a protein-protein interaction (PPI) network was constructed, and key genes were experimentally validated. These findings provide valuable insights into the core cell cycle gene family in M. polymorpha, contributing to an enhanced understanding of cell cycle regulation and its evolutionary significance in plants.

Keyword :

core cell cycle genes core cell cycle genes evolutionary analysis evolutionary analysis gene redundancy gene redundancy genome analysis genome analysis Marchantia polymorpha Marchantia polymorpha

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GB/T 7714 Chen, Xingguang , Feng, Haoran , Liu, Mengjuan et al. Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution [J]. | PHYSIOLOGIA PLANTARUM , 2025 , 177 (5) .
MLA Chen, Xingguang et al. "Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution" . | PHYSIOLOGIA PLANTARUM 177 . 5 (2025) .
APA Chen, Xingguang , Feng, Haoran , Liu, Mengjuan , Cai, Jiahao , Sarwar, Rabia , Li, Xueli et al. Genome-Wide Identification and Evolution of Core Cell Cycle Genes in Marchantia polymorpha: Insights Into Redundancy, Stress, and Functional Evolution . | PHYSIOLOGIA PLANTARUM , 2025 , 177 (5) .
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Heterologous overexpression of the Suaeda glauca stress-associated protein (SAP) family genes enhanced salt tolerance in Arabidopsis transgenic lines SCIE
期刊论文 | 2025 , 224 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
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Stress-associated proteins (SAPs), characterized by zinc finger domains, play a crucial role in regulating plant responses to various stresses. These proteins modulate stress-related gene expression and are integral to enhancing plant immunity, development, cell proliferation, and hormone regulation. In this study, we conducted a genome-wide analysis of the SAP gene family in Suaeda glauca (S. glauca), identifying 15 SAP genes encoding A20/AN1 zinc finger proteins. Functional analyses of three candidate genes under salinity stress were performed, examining phenotypic and physiological responses to better understand their role in stress tolerance. Sequence alignment, conserved domain analysis, and gene structure analysis revealed high conservation among S. glauca SAPs. Phylogenetic analysis identified two major groups within the gene family, providing insights into their evolutionary relationships. Transcription profiling analysis demonstrated significant expression of most SAP genes in response to salt stress, with qPCR validation confirming the upregulation of specific genes. Notably, transgenic Arabidopsis lines heterologously overexpressing the candidate genes SgSAP4, SgSAP5, and SgSAP7 demonstrated enhanced tolerance to salinity stress. This was evident from improved seed germination, root elongation, and reduced levels of stress markers, including malondialdehyde and free proline, compared to wildtype plants. These findings highlight the potential of these SAP genes in breeding programs aimed at improving salinity tolerance in crops.

Keyword :

Salinity stress Salinity stress SAP family SAP family Suaeda glauca Suaeda glauca Transgenic lines Transgenic lines

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GB/T 7714 Mohammadi, Mohammad Aqa , Wang, Yining , Zhang, Chunyin et al. Heterologous overexpression of the Suaeda glauca stress-associated protein (SAP) family genes enhanced salt tolerance in Arabidopsis transgenic lines [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 224 .
MLA Mohammadi, Mohammad Aqa et al. "Heterologous overexpression of the Suaeda glauca stress-associated protein (SAP) family genes enhanced salt tolerance in Arabidopsis transgenic lines" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 224 (2025) .
APA Mohammadi, Mohammad Aqa , Wang, Yining , Zhang, Chunyin , Ma, Haifeng , Sun, Jin , Wang, Lulu et al. Heterologous overexpression of the Suaeda glauca stress-associated protein (SAP) family genes enhanced salt tolerance in Arabidopsis transgenic lines . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 224 .
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Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues SCIE
期刊论文 | 2025 , 26 (6) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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Clinacanthus nutans is a valuable traditional medicinal plant that contains enriched active compounds such as triterpenoids and flavonoids. Understanding the accuulation process of these secondary metabolites in C. nutans requires exploring gene expression regulation under abiotic stresses and hormonal stimuli. qRT-PCR is a powerful method for gene expression analysis, with the selection of suitable reference genes being paramount. However, reports on stably expressed reference genes in C. nutans and even across the entire family Acanthaceae are limited. In this study, we evaluated the expression stability of 12 candidate reference genes (CnUBQ, CnRPL, CnRPS, CnPTB1, CnTIP41, CnACT, CnUBC, CnGAPDH, Cn18S, CnCYP, CnEF1 alpha, and CnTUB) in C. nutans across different tissues and under abiotic stresses and MeJA treatment using three programs (geNorm, NormFinder, and BestKeeper). The integrated ranking results indicated that CnUBC, CnRPL, and CnCYP were the most stably expressed genes across different tissues. Under abiotic stress conditions, CnUBC, CnRPL, and CnEF1 alpha were the most stable, while under MeJA treatment, CnRPL, CnEF1 alpha, and CnGAPDH exhibited the highest stability. Additionally, CnRPL, CnUBC, and CnEF1 alpha were the most stable reference genes across all tested samples, whereas CnGAPDH was the least stable. CnRPL, consistently ranking among the top three most stable genes, may therefore serve as an ideal reference gene for qRT-PCR analysis in C. nutans. To further validate the selected reference genes, we assessed the expression of two key biosynthetic genes, CnPAL and CnHMGR. The results confirmed that using the most stable reference genes yielded expression patterns consistent with biological expectations, while using unstable reference genes led to significant deviations. These findings offer valuable insights for accurately quantifying target genes via qRT-PCR in C. nutans, facilitating investigations into the mechanisms underlying active compound accumulation.

Keyword :

Clinacanthus nutans Clinacanthus nutans molecular biology techniques molecular biology techniques plant stress response plant stress response quantitative real-time PCR quantitative real-time PCR reference genes reference genes

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GB/T 7714 An, Chang , Lu, Lin , Yao, Yixin et al. Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) .
MLA An, Chang et al. "Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 6 (2025) .
APA An, Chang , Lu, Lin , Yao, Yixin , Liu, Ruoyu , Cheng, Yan , Lin, Yanxiang et al. Selection and Validation of Reference Genes in Clinacanthus nutans Under Abiotic Stresses, MeJA Treatment, and in Different Tissues . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (6) .
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Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family SCIE
期刊论文 | 2025 , 16 | FRONTIERS IN PLANT SCIENCE
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The HKT protein family plays a vital role in plant responses to salt stress by mediating sodium (Na+) and potassium (K+) transport and maintaining Na+-K+ balance. Ipomoea pes-caprae (IPC), a pantropical creeping plant distributed along coastal regions in tropical and subtropical zones, exhibits exceptional salt tolerance. Understanding its salt tolerance mechanisms provides valuable insights for developing salt-tolerant crops and identifying candidate genes for genetic engineering. In this study, we identified two HKT genes, IpcHKT1;1 and IpcHKT1;2, in IPC. Phylogenetic analysis with HKT genes from other Ipomoea species revealed that all analyzed species contain two HKT genes located adjacently on the same chromosome. Comparative analysis of conserved motifs and intron-exon structures indicated that, despite their close evolutionary relationship, the HKT genes in IPC may exhibit functional divergence. Promoter analysis showed that their regulatory regions are enriched with cis-elements associated with responses to biotic and abiotic stresses, hormonal signaling, and growth, highlighting functional diversity within the HKT family. Subcellular localization experiments demonstrated that IpcHKT1;1 and IpcHKT1;2 are ion transporters localized to the plasma membrane. Heterologous expression in yeast confirmed their role in Na+/K+ symporter. Furthermore, RT-qPCR analysis revealed distinct expression patterns under salt stress: IpcHKT1;2 was significantly upregulated in roots, while IpcHKT1;1 expression was transitionally downregulated at 400 mM NaCl treatment. Prolonged high expression of IpcHKT1;2 in roots suggests its critical role in sustained salt stress tolerance. These findings provide new insights into the molecular mechanisms of salt tolerance in IPC. The identification of IpcHKT1;1 and IpcHKT1;2 as key players in salt stress responses offers promising genetic resources for enhancing crop resilience to soil salinity, addressing challenges associated with global salinization.

Keyword :

halophytes halophytes HKT HKT Ipomoea pes-caprae Ipomoea pes-caprae salt stress salt stress sodium-potassium transport sodium-potassium transport

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GB/T 7714 Guo, Zhonghua , Sun, Jin , Chen, Xingguang et al. Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family [J]. | FRONTIERS IN PLANT SCIENCE , 2025 , 16 .
MLA Guo, Zhonghua et al. "Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family" . | FRONTIERS IN PLANT SCIENCE 16 (2025) .
APA Guo, Zhonghua , Sun, Jin , Chen, Xingguang , Li, Hui , Liang, Sisi , Liu, Fengying et al. Comparative analysis of HKT genes in Ipomoea pes-caprae unveils conserved Na+/K+ symporter functions within the gene family . | FRONTIERS IN PLANT SCIENCE , 2025 , 16 .
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Genome-Wide Identification and Analysis of the WUSCHEL-Related Homeobox (WOX) Gene Family in Passion Fruit (Passiflora edulis) SCIE
期刊论文 | 2025 , 15 (12) | AGRONOMY-BASEL
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The WUSCHEL-related homeobox (WOX) transcription factors (TF) regulate critical developmental processes in plants, including organ formation and stem cell maintenance. Although characterized in model species, the WOX family remains unexplored in passion fruit (Passiflora edulis). In this study, 10 WOX genes were identified in passion fruit, which are distributed across six chromosomes. We analyzed the phylogenetic relationships, gene structure, conserved motifs, and syntenic relationships of the PeWOX genes. Multiple sequence alignment analysis revealed strong conservation of the homeodomain region among WOX TF family members. Phylogenetic reconstruction further demonstrated that the 10 identified PeWOX genes in passion fruit could be classified into three distinct evolutionary clades: the WUS clade, the Intermediate clade, and the Ancient clade. The conserved motif and gene structure of WOX TF family members in the same evolutionary clade were highly consistent. Expression analysis based on RNA-seq and RT-qPCR showed that most PeWOX genes were expressed during ovule development. The expression level of PeWOX genes varies with different stress conditions. Subcellular localization analysis of tobacco leaf epidermal cells showed that PeWOX3/7/10 proteins were localized in the nucleus and cell membrane. Collectively, this study lays a foundation for future functional studies of passion fruit WOX genes.

Keyword :

developmental analysis developmental analysis homeobox genes homeobox genes passion fruit passion fruit WOX transcription factor WOX transcription factor

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GB/T 7714 Gao, Jingai , Zhang, Dan , Xu, Lixin et al. Genome-Wide Identification and Analysis of the WUSCHEL-Related Homeobox (WOX) Gene Family in Passion Fruit (Passiflora edulis) [J]. | AGRONOMY-BASEL , 2025 , 15 (12) .
MLA Gao, Jingai et al. "Genome-Wide Identification and Analysis of the WUSCHEL-Related Homeobox (WOX) Gene Family in Passion Fruit (Passiflora edulis)" . | AGRONOMY-BASEL 15 . 12 (2025) .
APA Gao, Jingai , Zhang, Dan , Xu, Lixin , Wu, Ting , Olunuga, Omotola Adebayo , Arabzai, Mohammad Gul et al. Genome-Wide Identification and Analysis of the WUSCHEL-Related Homeobox (WOX) Gene Family in Passion Fruit (Passiflora edulis) . | AGRONOMY-BASEL , 2025 , 15 (12) .
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Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response SCIE
期刊论文 | 2025 , 25 (1) | BMC PLANT BIOLOGY
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BackgroundPineapple (Ananas comosus L.), an economically significant tropical fruit crop, is highly susceptible to low-temperature during cultivation. The abscisic acid (ABA) and stress-inducible HVA22 gene family is known to play important roles in growth regulation and abiotic stress response, but its functions in pineapple remain unclear.ResultsWe identified 11 HVA22 genes (AcHVA22A - AcHVA22K) in the pineapple genome and grouped them into four phylogenetic clades. Expression analysis showed that most AcHVA22 genes displayed tissue- or developmental stage-specific expression patterns, suggesting their diverse functions in pineapple growth and development. Subcellular localization analyses revealed diverse localizations of AcHVA22 proteins, including plasma membrane, cytoplasm, and nucleus. And regulatory predictions indicated control by multiple cis-elements, transcription factors, and miRNAs, which may contribute to their functional diversification. Most AcHVA22 genes responded consistently to ABA, GA, and drought treatments, but their responses to high and low-temperatures varied. Notably, AcHVA22C/D/E/G/I/K were up-regulated under both short- and long-term cold treatments in two different pineapple varieties, highlighting their potential key roles in cold stress tolerance.ConclusionsThis study provided the first genome-wide characterization of the HVA22 gene family in pineapple. The identification of candidate genes involved in cold stress response offers new insights into HVA22 functions in tropical fruits and provides valuable resources for improving cold resistance in pineapple breeding.

Keyword :

Cold response Cold response Expression profiling Expression profiling HVA22 genes HVA22 genes Pineapple Pineapple Tissue-specific Tissue-specific

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GB/T 7714 Hou, Zhimin , Cai, Xinkai , Wu, Jiahao et al. Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response [J]. | BMC PLANT BIOLOGY , 2025 , 25 (1) .
MLA Hou, Zhimin et al. "Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response" . | BMC PLANT BIOLOGY 25 . 1 (2025) .
APA Hou, Zhimin , Cai, Xinkai , Wu, Jiahao , Lu, Lin , Liu, Chaojia , Zhang, Yangmei et al. Identification and characterization of HVA22 genes in pineapple (Ananas comosus L.) revealed their potential roles in development regulation and cold stress response . | BMC PLANT BIOLOGY , 2025 , 25 (1) .
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Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis SCIE
期刊论文 | 2025 , 177 (6) | PHYSIOLOGIA PLANTARUM
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Environmental constraints such as drought and salt stress severely limit crop production worldwide. The WRKY TFs are very important in regulating plant growth and stress responses. Pineapple (Ananas comosus) is widely grown due to its unique flavor, high vitamins, and dietary fiber; however, the functional characterization of WRKY TFs in pineapple remains largely unexplored. In our study, we amplified and explored the molecular function of pineapple AcWRKY27, including its conserved domain, protein localization, transcriptional activity, and expression profiles in different tissues and stress treatments. Overexpression of AcWRKY27 in both rice and Arabidopsis thaliana (A. thaliana) resulted in growth inhibition, a decrease in primary root elongation, and a reduction in fresh weight under salt, drought, and ABA stress. RNA-Seq analysis and quantitative PCR (RT-qPCR) revealed that AcWRKY27 overexpression resulted in decreased expression levels of stress-responsive and ABA signaling pathway genes. These findings provide new perspectives on pineapple WRKY TFs and lay a foundation for improving pineapple stress tolerance through molecular breeding in the future.

Keyword :

ABA ABA AcWRKY AcWRKY salt and drought stress salt and drought stress transcription factor transcription factor

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GB/T 7714 Chai, Gaifeng , Huang, Dongping , Gao, Jingai et al. Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis [J]. | PHYSIOLOGIA PLANTARUM , 2025 , 177 (6) .
MLA Chai, Gaifeng et al. "Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis" . | PHYSIOLOGIA PLANTARUM 177 . 6 (2025) .
APA Chai, Gaifeng , Huang, Dongping , Gao, Jingai , Wu, Ting , Xu, Lixin , Arabzai, Mohammad Gul et al. Ectopic Expression of Pineapple AcWRKY27 Increases Sensitivity to Abiotic Stress in Rice and Arabidopsis . | PHYSIOLOGIA PLANTARUM , 2025 , 177 (6) .
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A highly efficient organogenesis system based on 6-benzylaminopurine and indole-6-butyric acid in Suaeda glauca- a medicinal halophyte under varying photoperiods SCIE
期刊论文 | 2024 , 216 | INDUSTRIAL CROPS AND PRODUCTS
WoS CC Cited Count: 2
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Suaeda glauca (Bunge) is a pivotal halophyte species, well-known for its rich nutrient content and its importance as a superior vegetable and oil crop, offering significant health benefits for both human and animal consumption. However, the absence of an efficient regeneration technique has severely hindered its genetic development. Consequently, there is an urgent need to establish an effective in vitro organogenesis initiation method for this valuable plant. In this study, our primary objective was to develop a comprehensive protocol for micropropagating S. glauca , primarily capitalizing on the synergistic effects of 6-benzylaminopurine (6-BAP) and indole-6-butyric acid (IBA). We explored the impact of different combinations of 6-benzylaminopurine (6-BAP), 2,4-dichloroacetic acid (2,4-D), and IBA on callus induction, organogenesis, and rooting using various explants (cotyledons, hypocotyls, and leaves) under two different photoperiod regimes during in vitro culture. Our results unveiled noteworthy variations in several variables related to organogenesis. The optimal combination of plant growth regulators (PGRs) for inducing embryonic callus from cotyledon (COT) was pinpointed at 4.44 mu M 6-BAP and 2.71 mu M 2,4-D. By employing 6-BAP concentrations ranging from 8.88 mu M to 13.33 mu M, alongside 0.49 mu M IBA, 3% sucrose, and 0.3% phytagel in full MS media under light conditions, we achieved a remarkable 100% regeneration rate through the direct organogenesis pathway. Our findings underscore the pivotal role of 6-BAP in triggering multiple shoots and attaining a 3.6% callus differentiation rate, while 0.98 mu M IBA resulted in a 65.28% root induction rate, facilitating the successful acclimatization of plants. Based on the synergistic effects of 6-BAP and IBA, we propose a robust protocol for S. glauca organogenesis, which holds great promise for streamlining micropropagation in S. glauca tissue culture. This standardized protocol has the potential to advance genetic improvement and promote the commercial cultivation of S. glauca as a valuable halophyte species.

Keyword :

Callus Callus Organogenesis Organogenesis Photoperiods Photoperiods Plant growth regulators Plant growth regulators Suaeda glauca Suaeda glauca

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GB/T 7714 Mohammadi, Mohammad Aqa , Xu, Mengjie , Wang, Yining et al. A highly efficient organogenesis system based on 6-benzylaminopurine and indole-6-butyric acid in Suaeda glauca- a medicinal halophyte under varying photoperiods [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2024 , 216 .
MLA Mohammadi, Mohammad Aqa et al. "A highly efficient organogenesis system based on 6-benzylaminopurine and indole-6-butyric acid in Suaeda glauca- a medicinal halophyte under varying photoperiods" . | INDUSTRIAL CROPS AND PRODUCTS 216 (2024) .
APA Mohammadi, Mohammad Aqa , Xu, Mengjie , Wang, Yining , Zhang, Zihao , Wai, Myat Hnin , Rizwan, Hafiz Muhammad et al. A highly efficient organogenesis system based on 6-benzylaminopurine and indole-6-butyric acid in Suaeda glauca- a medicinal halophyte under varying photoperiods . | INDUSTRIAL CROPS AND PRODUCTS , 2024 , 216 .
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