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学者姓名：彭露
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Abstract ：
Insect seminal fluid proteins (SFPs) are primary factors affecting physiology and behavior in both sexes, making them valuable targets for pest control. However, SFPs have not been fully characterized in the Plutella xylostella, a global pest that attacks cruciferous crops. Here, 75 putative SFPs were identified in P. xylostella, compared to 10 orthologs in Drosophila melanogaster, 10 in Nilaparvata lugens, 5 in Apis mellifera, and 43 in Heliconius melpomene. Analyses of Ka/Ks suggested that SFPs had high evolution rates. Proteases (22/75, 29.3 %) accounted for the highest proportion of P. xylostella SFPs, including 16 trypsins. The phylogenetic analysis showed that most trypsins from P. xylostella and H. melpomene belonged to the same cluster. SFP04 (trypsin-1) was orthologous to the SFP ADJ58550.1 in H. melpomene. PxTry1 was specifically expressed in adult males and their accessory glands but was also detected in females after mating. A CRISPR/Cas9-induced PxTry1 homozygous mutant strain with a 22-base pair nucleotides insertion was generated. PxTry1 deletion resulted in swollen testes, smaller spermatophores, and abnormal sperm, thus reducing the P. xylostella egg production and hatching. These results clarify the role of insect SFPs in evolution and reproduction and identify a promising target for pest control based on genetic regulation.
Keyword ：
Genetic control Genetic control Plutella xylostella Plutella xylostella Seminal fluid protein Seminal fluid protein Spermatogenesis Spermatogenesis Trypsin Trypsin
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| GB/T 7714 | Peng, Lu , Zheng, Jun-Hao , Liu, Li-Li et al. Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 306 . |
| MLA | Peng, Lu et al. "Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 306 (2025) . |
| APA | Peng, Lu , Zheng, Jun-Hao , Liu, Li-Li , Huang, Meng-Qi , Cao, Min-Hui , Cui, Jin-Dong et al. Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 306 . |
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Mating induces post-mating responses in female insects, causing molecular, physiological, and behavioral changes. Spermatophore is a unique structure in females for seminal fluid storage in the bursa copulatrix, significant in sperm transfer and activation, and its digestion represents a crucial signal for female re-mating. Plutella xylostella, a lepidopteran pest that attacks cruciferous crops and wild plants, is a promiscuous insect that digests spermatophores rapidly. Molecular mechanisms underlying spermatophore digestion in P. xylostella are poorly understood. This study aimed to identify post-mating response proteins by using isobaric tags for relative and absolute quantification proteomics. A total of 843 proteins had significant differential expression between the reproductive glands of virgin and mated females, 720 of which were significantly upregulated after mating, including a cysteine protease family gene, cysteine protease ervata-min-B-like (PxCPEB), which is potentially related to spermatophore digestion. PxCPEB was specifically expressed in P. xylostella females, mostly in the female lower reproductive gland, but only in the bursa copulatrix and spermatheca, and PxCPEB transcription was significantly increased after mating. CRISPR/Cas9-induced PxCPEB knockout successfully produced a homozygous mutant strain with a 22-bp deletion. PxCPEB loss did not affect spermatogenesis but significantly prolonged the rate of spermatophore digestion, increased the apoptosis level of the bursa copulatrix, and inhibited sperm release and re-mating in females, significantly decreasing female fertility. PxCPEB, as a regulator of P. xylostella post-mating responses, is critical in spermatophore digestion, sperm release, re-mating, and female fertility and its potential as a promising target for genetic regulation-based pest control.
Keyword ：
bursa copulatrix bursa copulatrix Plutellidae Plutellidae post-mating responses post-mating responses reproductive regulation reproductive regulation spermatheca spermatheca
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| GB/T 7714 | Zheng, Junhao , Zou, Mingmin , Qian, Yurui et al. Cysteine protease ervatamin-B-like-mediated spermatophore digestion and sperm release impair fertility of Plutella xylostella females [J]. | ENTOMOLOGIA GENERALIS , 2025 . |
| MLA | Zheng, Junhao et al. "Cysteine protease ervatamin-B-like-mediated spermatophore digestion and sperm release impair fertility of Plutella xylostella females" . | ENTOMOLOGIA GENERALIS (2025) . |
| APA | Zheng, Junhao , Zou, Mingmin , Qian, Yurui , Li, Ruonan , Bian, Huimin , Cao, Minhui et al. Cysteine protease ervatamin-B-like-mediated spermatophore digestion and sperm release impair fertility of Plutella xylostella females . | ENTOMOLOGIA GENERALIS , 2025 . |
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During oogenesis, the cell cycle becomes arrested during the DNA replication phase and resumes after mating facilitating crucial processes, including vitellogenesis and chorion formation. However, the molecular mechanisms regulating oogenesis in Plutella xylostella (L.) remain largely unexplored. Here, comparative transcriptomics analysis identified 1200 differentially expressed genes induced by mating. Among the 769 upregulated genes, minichromosome maintenance protein complex 10 (MCM10) may be a potential target for post-mating DNA replication initiation. The P. xylostella genome encodes 11 MCM genes. Phylogenetic analysis indicated that MCM proteins are relatively conserved across species but form distinct clades. PxMCM10 exhibited expression specificity in adult females and eggs, particularly in the ovaries. Furthermore, PxMCM10 knockdown impaired DNA replication initiation and oocyte development while increasing apoptosis in follicle cells. This downregulated vitellogenin, vitellogenin receptor, and chorion gene transcription, resulted in abnormal ovarian development, defective vitellogenesis, and impaired chorion formation. Hence, PxMCM10 may regulate DNA replication reinitiation post-mating by influencing follicle cell function to control chorion formation. We offer insights into the molecular mechanisms governing oogenesis in P. xylostella and other lepidopteran pests. Furthermore, PxMCM10 could be a potential target for innovative genetic strategies in pest management.
Keyword ：
DNA replication DNA replication Minichromosome maintenance protein Minichromosome maintenance protein Oogenesis Oogenesis Pest control Pest control Plutella xylostella Plutella xylostella
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| GB/T 7714 | Peng, Lu , Tun, Laiwai , Qian, Yurui et al. Mating-induced DNA replication factor minichromosome maintenance protein 10 promotes oogenesis in Plutella xylostella [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 320 . |
| MLA | Peng, Lu et al. "Mating-induced DNA replication factor minichromosome maintenance protein 10 promotes oogenesis in Plutella xylostella" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 320 (2025) . |
| APA | Peng, Lu , Tun, Laiwai , Qian, Yurui , Zhang, Guofeng , Cui, Jindong , Cao, Minhui et al. Mating-induced DNA replication factor minichromosome maintenance protein 10 promotes oogenesis in Plutella xylostella . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 320 . |
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本发明公开了十一醛在引诱红火蚁中的应用。本发明发现红火蚁尸堆中的挥发物十一醛对红火蚁具有引诱作用，结果表明：0.1μg/μL‑10μg/μL的十一醛能够明显引诱红火蚁，使其发生堆尸行为。因此，利用十一醛开发红火蚁引诱剂并施用于红火蚁分布密集的区域，能够诱集红火蚁，用于红火蚁的高效监测与防控。
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| GB/T 7714 | 彭露 , 刘峰豪 , 徐冯超 . 十一醛在引诱红火蚁中的应用 : CN202411355534.6[P]. | 2024-09-27 . |
| MLA | 彭露 et al. "十一醛在引诱红火蚁中的应用" : CN202411355534.6. | 2024-09-27 . |
| APA | 彭露 , 刘峰豪 , 徐冯超 . 十一醛在引诱红火蚁中的应用 : CN202411355534.6. | 2024-09-27 . |
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为明确不同环境中小叶女贞上烟粉虱种群的发生消长动态、空间分布规律，系统调查了云南省昆明市3种环境（盘龙江边、小康大道旁、云南农大校园）中小叶女贞上的烟粉虱成虫、若虫和卵的种群动态。在烟粉虱各发生时期，盘龙江边小叶女贞上烟粉虱若虫和卵的种群数量均为最多。在盛发期，盘龙江边烟粉虱成虫数为36.37头/叶，分别是小康大道旁和云南农大校园的1.27倍和2.54倍，各调查点的雌成虫发生数量约为雄成虫的2倍；盘龙江边烟粉虱若虫数为149.08头/叶，分别是小康大道旁和云南农大校园的1.78倍和1.99倍。在整个发生期的3种环境中，烟粉虱成虫和卵以在小叶女贞上部叶片分布最多、下部最少，若虫则在中部叶片分布最多、上部和下部叶片最少。研究结果可为不同环境园林植物上烟粉虱种群的预测预报和综合治理提供依据。
Keyword ：
发生规律 发生规律 小叶女贞 小叶女贞 烟粉虱 烟粉虱 种植环境 种植环境 空间分布 空间分布
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| GB/T 7714 | 孔娇 , 张薄 , 张金龙 et al. 云南昆明不同环境小叶女贞上烟粉虱种群动态 [J]. | 中国植保导刊 , 2025 , 45 (05) : 47-52 . |
| MLA | 孔娇 et al. "云南昆明不同环境小叶女贞上烟粉虱种群动态" . | 中国植保导刊 45 . 05 (2025) : 47-52 . |
| APA | 孔娇 , 张薄 , 张金龙 , 杨念 , 熊培炜 , 唐敏 et al. 云南昆明不同环境小叶女贞上烟粉虱种群动态 . | 中国植保导刊 , 2025 , 45 (05) , 47-52 . |
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BACKGROUN DPlutella xylostella (Linnaeus) is a destructive pest of cruciferous crops due to its strong reproductive capacity and extensive resistance to pesticides. Seminal fluid proteins (SFPs) are the main effective factors that determine the reproductive physiology and behaviour of both sexes. Although an increasing number of SFPs have been identified, the effects of astacins in SFPs on agricultural pests have not yet been reported. Here, we elucidated the mechanisms by which Sast1 (seminal astacin 1) regulates the fertility of Plutella xylostella (L.). RESULTS PxSast1 was specifically expressed in the testis and accesssory gland. CRISPR/Cas9-induced PxSast1 knockout successfully constructed two homozygous mutant strains. Sast1 impaired the fertility of P. xylostella by separately regulating the reproductive capacity of males and females. Loss of PxSast1, on the one hand, significantly decreased the ability of males to mate and fertilize, mainly manifested as shortened mating duration, reduced mating competitiveness and decreased eupyrene sperm production; on the other hand, it significantly inhibited the expression of chorion genes in females, resulting in oogenesis deficits. Simultaneously, for mated females, the differentially expressed genes in signalling pathways related to oogenesis and chorion formation were significantly enriched after PxSast1 knockout. CONCLUSION These analyses of the functions of PxSast1 as the regulator of spermatogenesis and oogenesis establish its importance in the fertility process of P. xylostella, as well as its potential as a promising target for genetic regulation-based pest control. (c) 2024 Society of Chemical Industry.
Keyword ：
astacin astacin chorion formation chorion formation eupyrene sperm eupyrene sperm fertility fertility seminal fluid proteins seminal fluid proteins
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| GB/T 7714 | Cao, Min-Hui , Zou, Ming-Min , Liu, Li-Li et al. Sast1-mediated manifold effects inhibit Plutella xylostella fertility [J]. | PEST MANAGEMENT SCIENCE , 2024 , 80 (6) : 2596-2609 . |
| MLA | Cao, Min-Hui et al. "Sast1-mediated manifold effects inhibit Plutella xylostella fertility" . | PEST MANAGEMENT SCIENCE 80 . 6 (2024) : 2596-2609 . |
| APA | Cao, Min-Hui , Zou, Ming-Min , Liu, Li-Li , Dong, Shi-Jie , Huang, Meng-Qi , Zheng, Jun-Hao et al. Sast1-mediated manifold effects inhibit Plutella xylostella fertility . | PEST MANAGEMENT SCIENCE , 2024 , 80 (6) , 2596-2609 . |
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The excessive and frequent use of insecticides has led to serious problems with insecticide residues, impacting nontarget organisms such as the parasitoid Encarsia formosa. This study examined the growth, development, and enzyme activity of E. formosa exposed to spirotetramat at LC10, LC30, and LC50. The regression equation for the toxicity of spirotetramat toward E. formosa was Y = 5.25X-11.07. After exposure to spirotetramat, the survival rates of E. formosa sharply decreased, which occurred earlier than those in the control batch. Although the maximum daily parasitism quantity of E. formosa increased and the average parasitism number, enumerated from the 1st to the 5th day, was 53.97 after being exposed to spirotetramat at LC10, the life span of its F1 generation adults was only 8.47 days, which was significantly shorter than that in the control batch. After being exposed to spirotetramat at LC50, the average parasitism number of E. formosa was 63.30, and the developmental time of its F1 generation, enumerated from the 1st to the 5th day after exposure to spirotetramat, was significantly longer than that of the control batch. The activities of mixed function oxidase, acetylcholinesterase, carboxylesterase, and catalase increased significantly, and the rate of increase in enzyme activity was directly proportional to the increase in the concentration of spirotetramat. These results revealed that the parasitic ability of E. formosa decreased after exposure to spirotetramat at LC10, 10 , LC30, 30 , and LC50. 50 . This leads to a change in parasitoid control of pests, revealing the potential environmental threat of insecticide residues to nontarget organisms.
Keyword ：
Detoxification enzymes Detoxification enzymes Ecological security Ecological security Parasitic ability Parasitic ability Parasitoid Parasitoid Protective enzymes Protective enzymes Spirotetramat Spirotetramat
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| GB/T 7714 | Yang, Shao-Wu , Zhang, Bo , Tian, Ye et al. Effects of injudicious use of spirotetramat on Encarsia formosa's ability to control Bemisia tabaci [J]. | SCIENCE OF THE TOTAL ENVIRONMENT , 2024 , 950 . |
| MLA | Yang, Shao-Wu et al. "Effects of injudicious use of spirotetramat on Encarsia formosa's ability to control Bemisia tabaci" . | SCIENCE OF THE TOTAL ENVIRONMENT 950 (2024) . |
| APA | Yang, Shao-Wu , Zhang, Bo , Tian, Ye , Peng, Lu , Yuan, Guo-Ting , Chen, Guo-Hua et al. Effects of injudicious use of spirotetramat on Encarsia formosa's ability to control Bemisia tabaci . | SCIENCE OF THE TOTAL ENVIRONMENT , 2024 , 950 . |
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The red palm weevil (RPW) is an invasive pest that causes devastating damage to a variety of palm plants, which exhibit specific immune priming to Bacillus thuringiensis (Bt). However, immune priming in RPW may incur a high fitness cost, and its molecular signaling pathways have not yet been reported. Here, we investigated the effect of Bt priming on RPW development and subsequently analyzed the hormonal and immune-related molecular pathways influencing the fitness cost induced by Bt priming. Bt priming delayed the body weight gain of fifth-instar larvae and prolonged their developmental duration. Bt priming significantly reduced the 20-hydroxyecdysone (20E) content in RPW hemolymph, and the expression levels of the 20E biosynthesis-related genes SHADOW and SHADE were significantly downregulated. Furthermore, we analyzed Toll pathway genes influencing Bt priming and found that only Sp & auml;tzle (SPZ) transcription was significantly activated under Bt priming. After silencing SPZ expression, the negative effects of Bt priming on development, SHADOW expression, and 20E synthesis were eliminated, thereby suggesting that SPZ is a key molecular signal mediating developmental and immune trade-offs induced by Bt priming. Our results elucidate the molecular cascade pathway of immune priming and provide new targets for improving the efficiency of RPW biological controls.
Keyword ：
Bacillus thuringiensis Bacillus thuringiensis fitness cost fitness cost immune priming immune priming red palm weevil red palm weevil Sp & auml;tzle Sp & auml;tzle
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| GB/T 7714 | Li, Pengju , Zhang, He , Tan, Anran et al. Spätzle Regulates Developmental and Immune Trade-Offs Induced by Bacillus thuringiensis Priming in Rhynchophorus ferrugineus [J]. | INSECTS , 2024 , 15 (12) . |
| MLA | Li, Pengju et al. "Spätzle Regulates Developmental and Immune Trade-Offs Induced by Bacillus thuringiensis Priming in Rhynchophorus ferrugineus" . | INSECTS 15 . 12 (2024) . |
| APA | Li, Pengju , Zhang, He , Tan, Anran , Hu, Zhuolin , Peng, Lu , Hou, Youming . Spätzle Regulates Developmental and Immune Trade-Offs Induced by Bacillus thuringiensis Priming in Rhynchophorus ferrugineus . | INSECTS , 2024 , 15 (12) . |
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The red imported fire ant (Solenopsis invicta Buren) is a social pest species with a robust reproductive ability that causes extensive damage. Identification of the genes involved in queen fertility is critical in order to better understand the reproductive biology and screening for the potential molecular targets in S. invicta. Here, we used the mRNA deep sequencing (RNA-seq) approach to identify differentially expressed genes (DEGs) in the transcriptomes of three reproductive caste types of S. invicta, including queen (QA) and winged female (FA) and male (MA) ants. The genes that were specific to and highly expressed in the queens were then screened, and the Vg2 and Vg3 genes were chosen as targets to explore their functions in oogenesis and fertility. A minimum of 6.08 giga bases (Gb) of clean reads was obtained from all samples, with a mapping rate > 89.78%. There were 7524, 7133, and 977 DEGs identified in the MA vs. QA, MA vs. FA, and FA vs. QA comparisons, respectively. qRT-PCR was used to validate 10 randomly selected DEGs, including vitellogenin 2 (Vg2) and 3 (Vg3), and their expression patterns were mostly consistent with the RNA-seq data. The S. invicta Vgs included conserved domains and motifs that are commonly found in most insect Vgs. SiVg2 and SiVg3 were highly expressed in queens and winged females and were most highly expressed in the thorax, followed by the fat body, head, and epidermis. Evaluation based on a loss-of-function-based knockdown analysis showed that the downregulation of either or both of these genes resulted in smaller ovaries, less oogenesis, and less egg production. The results of transcriptional sequencing provide a foundation for clarifying the regulators of queen fertility in S. invicta. The functions of SiVg2 and SiVg3 as regulators of oogenesis highlight their importance in queen fecundity and their potential as targets of reproductive disruption in S. invicta control.
Keyword ：
fertility fertility oogenesis oogenesis Solenopsis invicta Solenopsis invicta transcriptome transcriptome Vgs Vgs
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| GB/T 7714 | Liu, Fenghao , Xu, Fengchao , Zhang, Yikun et al. Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (24) . |
| MLA | Liu, Fenghao et al. "Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 . 24 (2023) . |
| APA | Liu, Fenghao , Xu, Fengchao , Zhang, Yikun , Qian, Yurui , Zhang, Guofeng , Shi, Longqing et al. Comparative Analyses of Reproductive Caste Types Reveal Vitellogenin Genes Involved in Queen Fertility in Solenopsis invicta . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2023 , 24 (24) . |
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Simple Summary The red imported fire ant (Solenopsis invicta Buren) is an invasive species with a wide distribution range in China. Red imported fire ants nest in soil, have strong territorial behavior and aggression, and can cause harm to humans and livestock through their stinging and venom. This study examined the effects of red imported fire ant nests on soil properties and microbial communities in five different habitats. In each habitat, compared to the non-red imported fire ant nest control soils, pH and nitrogen content significantly increased in red imported fire ant nests, whereas the content of heavy metals, such as Cr, Pb, Cu, and Ni, significantly decreased. Although the composition, abundance, and diversity of bacterial communities of the control soils varied between habitats, bacterial community composition in red imported fire ant nest soils was similar. The abundance of Actinobacteria significantly increased in the nest soils of red imported fire ants. We believe that red imported fire ants deliberately increase the abundance of Actinobacteria to reduce the harm cause by pathogenic bacteria in the nests. This study provides reference data for the changes in soil physicochemical properties and microbial communities caused by the adaptations of red imported fire ants to different habitat soils. The red imported fire ant (Solenopsis invicta Buren) is a highly adaptable invasive species that can nest and reproduce in different habitat soils. We aimed to explore the adaptability of red imported fire ants in different habitats by analyzing changes in the physicochemical properties of nest soils and bacterial communities. Five habitat types (forest, tea plantation, rice field, lawn, and brassica field) were selected. The results showed that the pH of the nest soils increased significantly in all five habitats compared to the control soils of the same habitat. A significant increase in nitrogen content was detected in the nests. The Cr, Pb, Cu, and Ni levels were significantly reduced in the soils of the five habitats, due to nesting activities. Analysis of the composition and diversity of the soil microbial community showed that, although the richness and diversity of bacteria in the nest soils of red imported fire ants in the five habitats varied, the relative abundance of Actinobacteria significantly increased and it emerged as the dominant bacterial group. These results indicate that red imported fire ants modify the physicochemical properties of nest soils and bacterial communities to create a suitable habitat for survival and reproduction.
Keyword ：
Actinobacteria Actinobacteria eusocial insect eusocial insect heavy metal heavy metal invasive species invasive species pH value pH value
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| GB/T 7714 | Shi, Longqing , Liu, Fenghao , Peng, Lu . Impact of Red Imported Fire Ant Nest-Building on Soil Properties and Bacterial Communities in Different Habitats [J]. | ANIMALS , 2023 , 13 (12) . |
| MLA | Shi, Longqing et al. "Impact of Red Imported Fire Ant Nest-Building on Soil Properties and Bacterial Communities in Different Habitats" . | ANIMALS 13 . 12 (2023) . |
| APA | Shi, Longqing , Liu, Fenghao , Peng, Lu . Impact of Red Imported Fire Ant Nest-Building on Soil Properties and Bacterial Communities in Different Habitats . | ANIMALS , 2023 , 13 (12) . |
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