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学者姓名：倪林
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Abstract ：
本发明公开了药物提取技术领域的一种福建柏叶提取物的制备方法及其应用，包括以下步骤：1)取收集的新鲜福建柏叶，经50‑60℃烘干后粉碎，过20目‑50目筛，得干燥的粉末；2)步骤1)所得粉末1000g，加入体积浓度为55‑95％乙醇溶液14500mL，提取温度为70‑90℃并回流提取40‑80min；3)将步骤2)所得物在超声功率为300‑500W条件下提取0.5‑3H，然后过滤，收集提取液，减压浓缩，得福建柏叶浸膏；4)将步骤3)所得福建柏叶浸膏与等量硅藻土拌样后用乙酸乙酯萃取，收集该部分萃取液，减压浓缩回收溶剂，得福建柏叶提取物；福建柏叶制备所得的提取物在体外有显著的抑制α‑葡萄糖苷酶活性。
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| GB/T 7714 | 黄维 , 李欣瑜 , 龚维勇 et al. 一种福建柏叶提取物的制备方法及其应用 : CN202510084192.7[P]. | 2025-01-20 . |
| MLA | 黄维 et al. "一种福建柏叶提取物的制备方法及其应用" : CN202510084192.7. | 2025-01-20 . |
| APA | 黄维 , 李欣瑜 , 龚维勇 , 李渊顺 , 徐会有 , 倪林 . 一种福建柏叶提取物的制备方法及其应用 : CN202510084192.7. | 2025-01-20 . |
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本发明公开了抗氧化药物技术领域的一个木脂素类新化合物及其制备方法，名称为：(S)‑3‑(4‑羟基‑3‑甲氧基苯基)‑2‑(2‑羟基‑5‑3‑羟丙基)‑3‑甲氧基苯基丙酸甲酯，本发明新化合物(S)‑3‑(4‑羟基‑3‑甲氧基苯基)‑2‑(2‑羟基‑5‑3‑羟丙基)‑3‑甲氧基苯基丙酸甲酯的DPPH自由基清除IC50值低于对照品维生素C，显示新化合物(S)‑3‑(4‑羟基‑3‑甲氧基苯基)‑2‑(2‑羟基‑5‑3‑羟丙基)‑3‑甲氧基苯基丙酸甲酯具有显著的抗氧化活性，且优于维生素C，具有良好的市场开发价值。
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| GB/T 7714 | 倪林 , 刘世林 , 邱远望 et al. 一个木脂素类新化合物及其制备方法 : CN202411965155.9[P]. | 2024-12-30 . |
| MLA | 倪林 et al. "一个木脂素类新化合物及其制备方法" : CN202411965155.9. | 2024-12-30 . |
| APA | 倪林 , 刘世林 , 邱远望 , 邹立君 , 陈志鑫 . 一个木脂素类新化合物及其制备方法 : CN202411965155.9. | 2024-12-30 . |
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为优化福建柏叶总二萜提取工艺并研究其抑菌活性，通过响应面法对福建柏叶总二萜提取工艺进行优化，并采用菌丝生长速率法探究福建柏叶总二萜对6种植物病原真菌的抑制活性，以胶孢炭疽菌作为供试菌，进一步对菌丝生理生化指标的变化及保护酶活性进行测定。结果表明，福建柏叶总二萜的最佳工艺条件如下：使用无水乙醇作为提取溶剂，提取温度为79℃，提取时间为53 min，液料比为14.5 mL∶1 g。在此条件下，福建柏叶总二萜得率为36.8%。福建柏叶总二萜对胶孢炭疽菌抑制效果较好，EC50值为1.466 mg·mL
Keyword ：
EC EC 抑菌活性 抑菌活性 福建柏 福建柏
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| GB/T 7714 | 付硕硕 , 龚伟勇 , 林逸轩 et al. 福建柏叶总二萜提取工艺优化及抑菌活性研究 [J]. | 中国农业科技导报(中英文) , 2025 , 27 (05) : 146-155 . |
| MLA | 付硕硕 et al. "福建柏叶总二萜提取工艺优化及抑菌活性研究" . | 中国农业科技导报(中英文) 27 . 05 (2025) : 146-155 . |
| APA | 付硕硕 , 龚伟勇 , 林逸轩 , 李渊顺 , 刘澄灿 , 倪林 . 福建柏叶总二萜提取工艺优化及抑菌活性研究 . | 中国农业科技导报(中英文) , 2025 , 27 (05) , 146-155 . |
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研究三七内生真菌Nigrospora sp.的化学成分及其生物活性。应用硅胶柱色谱、高效液相色谱等多种色谱分离技术，从该菌株土豆液体发酵产物的乙酸乙酯提取物中分离得到3个聚酮类化合物，根据NMR、HR-ESI-MS、ECD等方法确定其结构，分别鉴定为(R)-2-methyl-4-(2-oxobutyl) cyclopent-2-en-1-one (1)、multiplolide A(2)、8-O-acetylmultiplolide(3)。其中，化合物1是一个新的环戊烯酮类衍生物，化合物2和3是十元大环内酯类衍生物，均为首次从Nigrospora sp.分离得到。抗菌活性实验结果表明，化合物1对大肠杆菌(Escherichia coli)具有微弱的抗菌活性，最小抑菌浓度为64μg/mL。
Keyword ：
Nigrospora sp. Nigrospora sp. 内生真菌 内生真菌 抗菌活性 抗菌活性 聚酮 聚酮
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| GB/T 7714 | 陈浩阳 , 张继宇 , 徐孟飞 et al. 三七内生真菌Nigrospora sp.中一个新的聚酮化合物 [J]. | 天然产物研究与开发 , 2025 , 37 (06) : 1051-1054 . |
| MLA | 陈浩阳 et al. "三七内生真菌Nigrospora sp.中一个新的聚酮化合物" . | 天然产物研究与开发 37 . 06 (2025) : 1051-1054 . |
| APA | 陈浩阳 , 张继宇 , 徐孟飞 , 付硕硕 , 徐会有 , 倪林 . 三七内生真菌Nigrospora sp.中一个新的聚酮化合物 . | 天然产物研究与开发 , 2025 , 37 (06) , 1051-1054 . |
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The essential oil of Litsea cubeba, extracted from its fruits, demonstrates significant antifungal activity against Fusarium verticillioides, with an EC50 value of 0.122 mu L/mL, comparable to the synthetic fungicide methyl thiophanate (EC50: 0.108 mu L/mL). Notably, at equivalent concentrations, the essential oil of L. cubeba exhibits 31.13% higher inhibitory efficiency than methyl thiophanate, highlighting its potential as an eco-friendly alternative. At 8 mu L/mL, the essential oil effectively controls mycelial growth on tomato surfaces through combined preventive and curative actions. Mechanistic studies reveal that the essential oil induces fungal cell death via multiple pathways, including disruption of cellular integrity and membrane architecture, ROS accumulation, DNA damage, elevated MDA levels, and altered membrane permeability. Transcriptomic analysis further indicates that the essential oil of L. cubeba disrupts fungal oxidative homeostasis and modulates protective enzymes, leading to programmed cell death. These findings underscore the potential of the essential oil of L. cubeba as a sustainable antifungal agent with multifaceted mechanisms superior to conventional fungicides, providing a scientific basis for developing plant-based alternatives in agriculture.
Keyword ：
antifungal antifungal essential oil essential oil Fusarium Fusarium Litsea cubeba Litsea cubeba transcriptome transcriptome
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| GB/T 7714 | Wu, Kai-Wen , Sun, Qi , Wu, Zheng-Rong et al. Sustainable Antifungal Potential of the Essential Oil of Litsea cubeba: Multifaceted Mechanisms Targeting Fusarium verticillioides [J]. | JOURNAL OF CHEMISTRY , 2025 , 2025 (1) . |
| MLA | Wu, Kai-Wen et al. "Sustainable Antifungal Potential of the Essential Oil of Litsea cubeba: Multifaceted Mechanisms Targeting Fusarium verticillioides" . | JOURNAL OF CHEMISTRY 2025 . 1 (2025) . |
| APA | Wu, Kai-Wen , Sun, Qi , Wu, Zheng-Rong , Wu, Jian-Kai , Pan, Zhen-Ze , Godwinnnamdi, Njoku et al. Sustainable Antifungal Potential of the Essential Oil of Litsea cubeba: Multifaceted Mechanisms Targeting Fusarium verticillioides . | JOURNAL OF CHEMISTRY , 2025 , 2025 (1) . |
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This study optimized the extraction of sesamin and 4-hydroxy-sesamin from Cinnamomum camphora residue, yielding 0.3804 % under conditions of 86 degrees C, 43 min, and a 1:8.39 solid-liquid ratio. The purified compounds showed 67.86 % and 78.87 % recovery rates via recrystallization. In LPS-induced BV-2 microglia, 4hydroxy-sesamol (10-40 mu mol/L) activated autophagy, reduced iNOS, COX-2, IL-6, IL-1 beta, TNF-alpha mRNA expression, and demonstrated neuroprotection without cytotoxicity, suggesting its potential in mitigating neuroinflammation.
Keyword ：
anti-inflammatory activity anti-inflammatory activity Cinnamomum camphor Cinnamomum camphor response surface methodology response surface methodology silica gel column chromatography silica gel column chromatography
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| GB/T 7714 | Sun, Qi , Liu, Fen , Zhang, Jiyu et al. Sesamin and 4-hydroxy-sesamin from Cinnamomum camphora: extraction, purification and anti-inflammatory effects on the BV2 microglia by LPS-induced [J]. | JOURNAL OF CHEMICAL METROLOGY , 2025 , 19 (1) : 44-62 . |
| MLA | Sun, Qi et al. "Sesamin and 4-hydroxy-sesamin from Cinnamomum camphora: extraction, purification and anti-inflammatory effects on the BV2 microglia by LPS-induced" . | JOURNAL OF CHEMICAL METROLOGY 19 . 1 (2025) : 44-62 . |
| APA | Sun, Qi , Liu, Fen , Zhang, Jiyu , Song, Qiang , Xu, Mengfei , Chen, Mingfeng et al. Sesamin and 4-hydroxy-sesamin from Cinnamomum camphora: extraction, purification and anti-inflammatory effects on the BV2 microglia by LPS-induced . | JOURNAL OF CHEMICAL METROLOGY , 2025 , 19 (1) , 44-62 . |
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This study systematically investigated the optimization of total flavonoid extraction from the leaves of Bougainvillea glabra Choisy (B.glabra) and evaluated its antioxidant potential. The impact of three critical parameters (ultrasound time, heating reflux time, and ethanol concentration) on extraction efficiency was investigated using ultrasonication-assisted heat reflux extraction. A three-factor, three-level Box-Behnken design (BBD) was implemented to optimize the extraction procedure, using total flavonoid yield (TFY) as the response variable. The extracted flavonoids' antioxidant potential was measured quantitatively in vitro. The ideal conditions were 53% ethanol concentration, 10 minutes of ultrasonication, and 41 minutes of reflux. This procedure produced a TFY of (6.21 +/- 0.26)%. The extracts exhibited significant free radical scavenging action against 1,1-diphenyl-2-picryl-hydrazyl (DPPH) radical and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical. When the concentration exceeded 1.0 mg/ mL, the scavenging rate of DPPH free radicals surpassed 84.1%. When the concentration was more than 0.6 mg/ mL, the ability of total flavonoids to scavenge ABTS free radicals was over 93.8%, which was very similar to how well vitamin C worked. This optimized protocol proves effective for flavonoid extraction, with the obtained products exhibiting significant antioxidant properties in standardized assays.
Keyword ：
antioxidant activity. (c) 2025 ACG Publications. All rights reserved antioxidant activity. (c) 2025 ACG Publications. All rights reserved Bougainvillea Bougainvillea Box-Behnken response surface methodology Box-Behnken response surface methodology total flavonoids total flavonoids ultrasound-assisted extraction ultrasound-assisted extraction
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| GB/T 7714 | Yu, Jinghui , Dong, Bin , Zhao, Yanxin et al. Process optimization and antioxidant efficacy evaluation of total flavonoids from Bougainvillea glabra leaves: an integrated approach combining ultrasonic-assisted extraction with response surface methodology [J]. | JOURNAL OF CHEMICAL METROLOGY , 2025 , 19 (1) : 103-113 . |
| MLA | Yu, Jinghui et al. "Process optimization and antioxidant efficacy evaluation of total flavonoids from Bougainvillea glabra leaves: an integrated approach combining ultrasonic-assisted extraction with response surface methodology" . | JOURNAL OF CHEMICAL METROLOGY 19 . 1 (2025) : 103-113 . |
| APA | Yu, Jinghui , Dong, Bin , Zhao, Yanxin , Fang, Rong , Fu, Shuoshuo , Sun, Qi et al. Process optimization and antioxidant efficacy evaluation of total flavonoids from Bougainvillea glabra leaves: an integrated approach combining ultrasonic-assisted extraction with response surface methodology . | JOURNAL OF CHEMICAL METROLOGY , 2025 , 19 (1) , 103-113 . |
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本发明涉及一种福建柏叶提取物的制备方法及其在黑翅土白蚁生物防治中的应用。本发明设计得到福建柏叶提取物，并基于该福建柏叶提取物，通过混合搅拌的方式获得具有黑翅土白蚁防治效果的驱避剂，从而不管是福建柏叶提取物或得到的驱避剂均能够实现黑翅土白蚁的防治。本发明从植物化学角度深入挖掘和拓展了福建柏叶植物资源功效，促进了福建柏植物资源的保护和利用，所得的福建柏叶提取物和依此为基础的驱避剂对黑翅土白蚁具有显著忌避活性，可应用于林业及其他白蚁危害场所。具有绿色环保，对人体和环境的伤害小的优势，有较高的实用价值。本发明采用的制备方法简便且易操作，可适用于大规模生产，能够有效的降低生产成本。
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| GB/T 7714 | 倪林 , 龚伟勇 , 牛泽岳 et al. 一种福建柏叶提取物的制备方法及其在黑翅土白蚁生物防治中的应用 : CN202410495646.5[P]. | 2024-04-24 . |
| MLA | 倪林 et al. "一种福建柏叶提取物的制备方法及其在黑翅土白蚁生物防治中的应用" : CN202410495646.5. | 2024-04-24 . |
| APA | 倪林 , 龚伟勇 , 牛泽岳 , 吴建凯 , 李渊顺 , 廖鹏辉 et al. 一种福建柏叶提取物的制备方法及其在黑翅土白蚁生物防治中的应用 : CN202410495646.5. | 2024-04-24 . |
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本发明公开了一种福建柏叶提取物及其制备方法和在抑制植物病原真菌中的应用，其是将福建柏叶经烘干、粉碎后，加入乙醇溶液加热后回流提取，获得滤渣和提取液，再将滤渣再次加热回流提取，将两次的提取液减压通缩后与等量硅藻土拌样萃取、减压浓缩后获得福建柏叶提取物。本发明制备方法简单，易操作，其总得率在10.90‑11.80％之间，适合大规模生产，且所得提取物对植物病原真菌具有显著抑制活性，其在抑制胶孢炭疽菌、水稻稻瘟病菌、香蕉枯萎病菌方面的活性良好，优于目前大量使用的环境友好型植物源农药乙蒜素。
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| GB/T 7714 | 倪林 , 龚伟勇 , 付硕硕 et al. 一种福建柏叶提取物及其制备方法和在抑制植物病原真菌中的应用 : CN202410495650.1[P]. | 2024-04-24 . |
| MLA | 倪林 et al. "一种福建柏叶提取物及其制备方法和在抑制植物病原真菌中的应用" : CN202410495650.1. | 2024-04-24 . |
| APA | 倪林 , 龚伟勇 , 付硕硕 , 廖鹏辉 , 吴建凯 , 李渊顺 et al. 一种福建柏叶提取物及其制备方法和在抑制植物病原真菌中的应用 : CN202410495650.1. | 2024-04-24 . |
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| GB/T 7714 | Liu, Shilin , Fu, Shuoshuo , Fu, Yuping et al. Chemical Constituents from the Leaves of Cinnamomum longepaniculatum [J]. | CHEMISTRY OF NATURAL COMPOUNDS , 2024 , 60 (4) : 745-747 . |
| MLA | Liu, Shilin et al. "Chemical Constituents from the Leaves of Cinnamomum longepaniculatum" . | CHEMISTRY OF NATURAL COMPOUNDS 60 . 4 (2024) : 745-747 . |
| APA | Liu, Shilin , Fu, Shuoshuo , Fu, Yuping , Chen, Yuanan , Ni, Lin . Chemical Constituents from the Leaves of Cinnamomum longepaniculatum . | CHEMISTRY OF NATURAL COMPOUNDS , 2024 , 60 (4) , 745-747 . |
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