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学者姓名：尤民生
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Abstract ：
Insect seminal fluid proteins (SFPs) are primary factors affecting physiology and behavior in both sexes, making them valuable targets for pest control. However, SFPs have not been fully characterized in the Plutella xylostella, a global pest that attacks cruciferous crops. Here, 75 putative SFPs were identified in P. xylostella, compared to 10 orthologs in Drosophila melanogaster, 10 in Nilaparvata lugens, 5 in Apis mellifera, and 43 in Heliconius melpomene. Analyses of Ka/Ks suggested that SFPs had high evolution rates. Proteases (22/75, 29.3 %) accounted for the highest proportion of P. xylostella SFPs, including 16 trypsins. The phylogenetic analysis showed that most trypsins from P. xylostella and H. melpomene belonged to the same cluster. SFP04 (trypsin-1) was orthologous to the SFP ADJ58550.1 in H. melpomene. PxTry1 was specifically expressed in adult males and their accessory glands but was also detected in females after mating. A CRISPR/Cas9-induced PxTry1 homozygous mutant strain with a 22-base pair nucleotides insertion was generated. PxTry1 deletion resulted in swollen testes, smaller spermatophores, and abnormal sperm, thus reducing the P. xylostella egg production and hatching. These results clarify the role of insect SFPs in evolution and reproduction and identify a promising target for pest control based on genetic regulation.
Keyword ：
Genetic control Genetic control Plutella xylostella Plutella xylostella Seminal fluid protein Seminal fluid protein Spermatogenesis Spermatogenesis Trypsin Trypsin
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| GB/T 7714 | Peng, Lu , Zheng, Jun-Hao , Liu, Li-Li et al. Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 306 . |
| MLA | Peng, Lu et al. "Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 306 (2025) . |
| APA | Peng, Lu , Zheng, Jun-Hao , Liu, Li-Li , Huang, Meng-Qi , Cao, Min-Hui , Cui, Jin-Dong et al. Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 306 . |
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Fungus gnats, Lycoriella spp. (Diptera: Sciaridae), are significant pests in edible mushroom cultivation worldwide. This study evaluates the effectiveness of 2 soil predatory mites, Stratiolaelaps scimitus (Laelapidae) and Macrocheles glaber (Macrochelidae), in controlling fungus gnats density on the mushroom Agrocybe aegerita. A controlled experiment was performed to examine and compare the effects of individual and combined releases of the 2 mite species on fungus gnats populations and their impact on mushroom yield. Results showed that both mite species significantly reduced fungus gnat populations as compared to untreated controls. However, S. scimitus exhibited superior control, effectively suppressing adult fungus gnats compared to the controls, single release of M. glaber and combined releases (M. glaber + S. scimitus). Mushroom yield was highest in the S. scimitus treatment, with no significant differences observed among other treatments and controls. Interestingly, neither mite species negatively impacted mushroom quality or budding. These findings highlight the significance of S. scimitus as a highly effective biological control agent for suppression of fungus gnats, thereby contributing to sustainable pest management practices in mushroom cultivation.
Keyword ：
Agrocybe aegerita Agrocybe aegerita biological control biological control Laelapidae Laelapidae Macrochelidae Macrochelidae Sciaridae Sciaridae
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| GB/T 7714 | Lan, Qing-Xiu , Wen, Mei-Fang , Lu, Zheng-Hui et al. Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae) [J]. | INSECT SCIENCE , 2025 . |
| MLA | Lan, Qing-Xiu et al. "Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae)" . | INSECT SCIENCE (2025) . |
| APA | Lan, Qing-Xiu , Wen, Mei-Fang , Lu, Zheng-Hui , Ke, Bing-Rong , Fan, Qing-Hai , You, Min-Sheng . Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae) . | INSECT SCIENCE , 2025 . |
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The olfactory system of insects plays a pivotal role in multiple, essential activities including feeding, mating, egg laying, and host localization. The capacity of odorant receptors to recognize odor molecules relies on odorant receptor co-receptors forming heterodimers. Here we report the successful engineering a homozygous mutant strain of diamondback moth ( Plutella xylostella) in which the odorant receptor co-receptor PxOrco was silenced using CRISPR/Cas9. This insect is a globally important crop pest for which novel control methods are urgently required. Behavioral assays demonstrated that PxOrco knockout males exhibited abolished courtship behaviors, inability to mate, and loss of selective preference for P. xylostella's key sex pheromone components. Whilst female mating behavior and fecundity remained unaffected by PxOrco knockout, oviposition response to leaf alcohol, a key cue for normal oviposition behavior, was lost. Electroantennography revealed drastically reduced responses to sex pheromones and plant volatiles in PxOrco-deficient adults but food location by larvae was unaffected. Moreover, expression analysis of PxOrco- deficient pheromone receptors (PRs) indicated varied regulation patterns, with down-regulation observed in several PRs in both sexes. These findings underscore the critical role of PxOrco in regulating multiple olfactory aspects in P. xylostella, including feeding, mating, and host location. Our study identifies the potential of disrupting the Orco gene in this and other pest species to provide novel avenues for future pest control.
Keyword ：
Electroantennography Electroantennography Mating disruption Mating disruption Odorant receptor co-receptors (orco) Odorant receptor co-receptors (orco) Oviposition behavior Oviposition behavior Pheromone Pheromone
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| GB/T 7714 | Chen, Xuanhao , Yao, Shuyuan , Xie, Liangqian et al. Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest [J]. | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 177 . |
| MLA | Chen, Xuanhao et al. "Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest" . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 177 (2025) . |
| APA | Chen, Xuanhao , Yao, Shuyuan , Xie, Liangqian , Li, Jinyang , Xiong, Lei , Yang, Xiaozhen et al. Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 177 . |
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Global climate change is characterized by increased extreme temperatures affecting insects at all trophic levels. Zinc finger proteins (ZFPs) are key regulators of gene expression and cell differentiation in eukaryotes, essential for stress resistance in both animals and plants. Using CRISPR/Cas9 for gene deletion, this study predicted and examined the structure of ZFP320 in the diamondback moth (Plutella xylostella) and investigated its function in temperature stress response through a comprehensive age-stage, two-sex life table analysis. We found ZFP320 encodes a 387 amino acid protein (43 kDa) with no transmembrane domains, featuring a ZnF-C2H2 domain. Quantitative fluorescence analysis showed that ZFP320 expression increased under high temperatures. ZFP320 knockout altered antioxidant gene expression, resulting in higher levels of superoxide dismutase and catalase in mutant strains compared with wild-type strain. Life table analysis revealed that the mutant strains had shorter fecundity and oviposition periods under both normal and high temperatures. Additionally, mutant strains exhibited lower parameters (r, lambda, R0), as well as reduced survival rates and critical thermal maxima. Notably, PxZFP320 plays a crucial role in temperature adaptation, paving the way for future investigations on the significance of ZFPs in P. xylostella's temperature tolerance.
Keyword ：
age-stage two-sex life table age-stage two-sex life table CRISPR/Cas9 CRISPR/Cas9 Plutella xylostella Plutella xylostella temperature adaptability temperature adaptability
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| GB/T 7714 | Miao, Xin , Cao, Fang , Yu, Xiao-Fei et al. A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest [J]. | OPEN BIOLOGY , 2025 , 15 (4) . |
| MLA | Miao, Xin et al. "A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest" . | OPEN BIOLOGY 15 . 4 (2025) . |
| APA | Miao, Xin , Cao, Fang , Yu, Xiao-Fei , Li, Tian-Pu , Su, Hai-Yin , Guo, Jiao et al. A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest . | OPEN BIOLOGY , 2025 , 15 (4) . |
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Abstract ：
本实用新型涉及一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯，属于实验技术领域。通过一种自制的养虫杯，由两个一次性透明航空杯叠放组合而成，装置上部分的养虫杯顶端用纱网罩住，橡皮圈扎紧，作为透气口；底端中间位置钻孔，插入茶树嫩梢作为食源。装置下部分的养虫杯中盛2/3水，用脱脂棉包裹住茶枝与杯孔的缝隙处，防止叶蝉逃逸。本实用新型简单实用，价格低廉，体积轻巧，大大提高了观察效率的同时，减轻了饲养期间的工作量，便于重复性多，需虫量大的室内毒力测定实验。非常适合茶小绿叶蝉的室内饲养和科研用途研究，且可为其他昆虫的大规模饲养提供借鉴。
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| GB/T 7714 | 周敏 , 尤士骏 , 李金玉 et al. 一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯 : CN202323295506.3[P]. | 2023-12-04 . |
| MLA | 周敏 et al. "一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯" : CN202323295506.3. | 2023-12-04 . |
| APA | 周敏 , 尤士骏 , 李金玉 , 尤民生 . 一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯 : CN202323295506.3. | 2023-12-04 . |
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The fluctuation in temperature poses a significant challenge for poikilothermic organisms, notably insects, particularly in the context of changing climatic conditions. In insects, temperature adaptation has been driven by polygenes. In addition to genes that directly affect traits (core genes), other genes (peripheral genes) may also play a role in insect temperature adaptation. This study focuses on two peripheral genes, the GRIP and coiled-coil domain containing 2 (GCC2) and karyopherin subunit beta 1 (KPNB1). These genes are differentially expressed at different temperatures in the cosmopolitan pest, Plutella xylostella. GCC2 and KPNB1 in P. xylostella were cloned, and their relative expression patterns were identified. Reduced capacity for thermal adaptation (development, reproduction and response to temperature extremes) in the GCC2-deficient and KPNB1-deficient P. xylostella strains, which were constructed by CRISPR/Cas9 technique. Deletion of the PxGCC2 or PxKPNB1 genes in P. xylostella also had a differential effect on gene expression for many traits including stress resistance, resistance to pesticides, involved in immunity, trehalose metabolism, fatty acid metabolism and so forth. The ability of the moth to adapt to temperature via different pathways is likely to be key to its ability to remain an important pest species under predicted climate change conditions.
Keyword ：
CRISPR/Cas9 system CRISPR/Cas9 system peripheral genes peripheral genes Plutella xylostella Plutella xylostella regulatory network regulatory network temperature adaptability temperature adaptability
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| GB/T 7714 | Lei, Gaoke , Huang, Jieling , Zhou, Huiling et al. Polygenic adaptation of a cosmopolitan pest to a novel thermal environment [J]. | INSECT MOLECULAR BIOLOGY , 2024 , 33 (4) : 387-404 . |
| MLA | Lei, Gaoke et al. "Polygenic adaptation of a cosmopolitan pest to a novel thermal environment" . | INSECT MOLECULAR BIOLOGY 33 . 4 (2024) : 387-404 . |
| APA | Lei, Gaoke , Huang, Jieling , Zhou, Huiling , Chen, Yanting , Song, Jun , Xie, Xuefeng et al. Polygenic adaptation of a cosmopolitan pest to a novel thermal environment . | INSECT MOLECULAR BIOLOGY , 2024 , 33 (4) , 387-404 . |
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Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing. In the current study, we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella, in addition to its known functions in the ovarian development in female adults and egg hatching. Subsequent CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein (GFP) gene into autosomal Pxfl(2)d for labelling mutants could be achieved. However, this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive capacity. We further screened for the W-chromosomelinked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/Cas9. The results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous sequences. We ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein (CFP) expression cassettes on a Z-linked region in P. xylostella, which can also be used for early sex detection. By injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain, the gene editing efficiency reached 29.73%, confirming the success of expressing a functional Cas9 gene. Taken together, we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in P. xylostella, while the establishment of a heritable strain required the positioning of appropriate sites. This study provides an important working basis and technical support for further developing genetic strategies for insect pest control.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 cruciferous specialist cruciferous specialist fl(2)d fl(2)d knock-in knock-in sex chromosome sex chromosome
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| GB/T 7714 | Li, Shanyu , Lin, Guifang , Wen, Haoqi et al. Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth, Plutella xylostella [J]. | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (9) : 3089-3103 . |
| MLA | Li, Shanyu et al. "Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth, Plutella xylostella" . | JOURNAL OF INTEGRATIVE AGRICULTURE 23 . 9 (2024) : 3089-3103 . |
| APA | Li, Shanyu , Lin, Guifang , Wen, Haoqi , Lu, Haiyan , Yin, Anyuan , Zheng, Chanqin et al. Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth, Plutella xylostella . | JOURNAL OF INTEGRATIVE AGRICULTURE , 2024 , 23 (9) , 3089-3103 . |
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Understanding a population's fitness heterogeneity and genetic basis of thermal adaptation is essential for predicting the responses to global warming. We examined the thermotolerance and genetic adaptation of Plutella xylostella to exposure to hot temperatures. The population fitness parameters of the hot- acclimated DBM strains varied in the thermal environments. Using genome scanning and transcription profiling, we find a number of genes potentially involved in thermal adaptation of DBM. Editing two ABCG transporter genes, PxWhite and PxABCG, confirmed their role in altering cuticle permeability and influencing thermal responses. Our results demonstrate that SNP mutations in genes and changes in gene expression can allow DBM to rapidly adapt to thermal environment. ABCG transporter genes play an important role in thermal adaptation of DBM. This work improves our understanding of genetic adaptation mechanisms of insects to thermal stress and our capacity to predict the effects of rising global temperatures on ectotherms.
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| GB/T 7714 | You, Shijun , Lei, Gaoke , Zhou, Huiling et al. Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest [J]. | ISCIENCE , 2024 , 27 (3) . |
| MLA | You, Shijun et al. "Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest" . | ISCIENCE 27 . 3 (2024) . |
| APA | You, Shijun , Lei, Gaoke , Zhou, Huiling , Li, Jianyu , Chen, Shaoping , Huang, Jieling et al. Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest . | ISCIENCE , 2024 , 27 (3) . |
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Juvenile hormone binding protein (JHBP) is a key regulator of JH signaling, and crosstalk between JH and 20-hydroxyecdysone (20E) can activate and fine-tune the mitogen-activated protein kinase cascade, leading to resistance to insecticidal proteins from Bacillis thuringiensis (Bt). However, the involvement of JHBP in the Bt Cry1Ac resistance of Plutella xylostella remains unclear. Here, we cloned a full-length cDNA encoding JHBP, and quantitative real-time PCR (qPCR) analysis showed that the expression of the PxJHBP gene in the midgut of the Cry1Ac-susceptible strain was significantly higher than that of the Cry1Ac-resistant strain. Furthermore, CRISPR/Cas9-mediated knockout of the PxJHBP gene significantly increased Cry1Ac susceptibility, resulting in a significantly shorter lifespan and reduced fertility. These results demonstrate that PxJHBP plays a critical role in the resistance to Cry1Ac protoxin and in the regulation of physiological metabolic processes associated with reproduction in adult females, providing valuable insights to improve management strategies of P. xylostella.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 Cry1Ac Cry1Ac life tables life tables Plutella xylostella Plutella xylostella PxJHBP PxJHBP
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| GB/T 7714 | You, Shijun , Yao, Shuyuan , Chen, Xuanhao et al. CRISPR/Cas9-Mediated Knockout of the PxJHBP Gene Resulted in Increased Susceptibility to Bt Cry1Ac Protoxin and Reduced Lifespan and Spawning Rates in Plutella xylostella [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (14) : 8180-8188 . |
| MLA | You, Shijun et al. "CRISPR/Cas9-Mediated Knockout of the PxJHBP Gene Resulted in Increased Susceptibility to Bt Cry1Ac Protoxin and Reduced Lifespan and Spawning Rates in Plutella xylostella" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 14 (2024) : 8180-8188 . |
| APA | You, Shijun , Yao, Shuyuan , Chen, Xuanhao , Hou, Qing , Liu, Zhaoxia , Lei, Gaoke et al. CRISPR/Cas9-Mediated Knockout of the PxJHBP Gene Resulted in Increased Susceptibility to Bt Cry1Ac Protoxin and Reduced Lifespan and Spawning Rates in Plutella xylostella . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (14) , 8180-8188 . |
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BackgroundGlobal climate change significantly impacts ecosystems, particularly through temperature fluctuations that affect insect physiology and behavior. As poikilotherms, insect pests such as the globally devastating diamondback moth (DBM), Plutella xylostella, are especially vulnerable to rising temperatures and extreme heat events, necessitating effective adaptive mechanisms.ResultsHere we demonstrate the roles of zinc finger proteins (ZFPs) in mediating thermal adaptability in DBM. We utilized a comprehensive approach involving cloning and bioinformatics analysis of three ZFPs, PxZNF568, PxZNF93, and PxZNF266, measurement of their expression levels in hot-evolved and control strains, and assessment of catalase enzymatic activity and total antioxidant capacity. We also employed CRISPR/Cas9 technology to create five stable homozygous knockout strains to elucidate ZFP functions in high-temperature tolerance. Survival rates under high-temperature stress and the critical thermal maxima (CTMax) of the knockout strains were significantly lower than the wild-type strain, and exhibited marked decreases in antioxidant capacity.ConclusionFindings reveal the importance of ZFPs in thermal adaptability of DBM, contributing critical insights for future pest management strategies in the context of a warming climate and laying the foundation for further exploration of ZFP functionality in agricultural pest control.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 Plutella xylostella Plutella xylostella Temperature adaptability Temperature adaptability Zinc finger proteins Zinc finger proteins
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| GB/T 7714 | Li, Tianpu , Guo, Jiao , Hu, Guilei et al. Zinc finger proteins facilitate adaptation of a global insect pest to climate change [J]. | BMC BIOLOGY , 2024 , 22 (1) . |
| MLA | Li, Tianpu et al. "Zinc finger proteins facilitate adaptation of a global insect pest to climate change" . | BMC BIOLOGY 22 . 1 (2024) . |
| APA | Li, Tianpu , Guo, Jiao , Hu, Guilei , Cao, Fang , Su, Haiyin , Shen, Mengdi et al. Zinc finger proteins facilitate adaptation of a global insect pest to climate change . | BMC BIOLOGY , 2024 , 22 (1) . |
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