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学者姓名：尤民生
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Abstract ：
Insect seminal fluid proteins (SFPs) are primary factors affecting physiology and behavior in both sexes, making them valuable targets for pest control. However, SFPs have not been fully characterized in the Plutella xylostella, a global pest that attacks cruciferous crops. Here, 75 putative SFPs were identified in P. xylostella, compared to 10 orthologs in Drosophila melanogaster, 10 in Nilaparvata lugens, 5 in Apis mellifera, and 43 in Heliconius melpomene. Analyses of Ka/Ks suggested that SFPs had high evolution rates. Proteases (22/75, 29.3 %) accounted for the highest proportion of P. xylostella SFPs, including 16 trypsins. The phylogenetic analysis showed that most trypsins from P. xylostella and H. melpomene belonged to the same cluster. SFP04 (trypsin-1) was orthologous to the SFP ADJ58550.1 in H. melpomene. PxTry1 was specifically expressed in adult males and their accessory glands but was also detected in females after mating. A CRISPR/Cas9-induced PxTry1 homozygous mutant strain with a 22-base pair nucleotides insertion was generated. PxTry1 deletion resulted in swollen testes, smaller spermatophores, and abnormal sperm, thus reducing the P. xylostella egg production and hatching. These results clarify the role of insect SFPs in evolution and reproduction and identify a promising target for pest control based on genetic regulation.
Keyword ：
Genetic control Genetic control Plutella xylostella Plutella xylostella Seminal fluid protein Seminal fluid protein Spermatogenesis Spermatogenesis Trypsin Trypsin
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| GB/T 7714 | Peng, Lu , Zheng, Jun-Hao , Liu, Li-Li et al. Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 306 . |
| MLA | Peng, Lu et al. "Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 306 (2025) . |
| APA | Peng, Lu , Zheng, Jun-Hao , Liu, Li-Li , Huang, Meng-Qi , Cao, Min-Hui , Cui, Jin-Dong et al. Identification of seminal fluid proteins and reproductive function of trypsin-1 in male Plutella xylostella . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 306 . |
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The olfactory system of insects plays a pivotal role in multiple, essential activities including feeding, mating, egg laying, and host localization. The capacity of odorant receptors to recognize odor molecules relies on odorant receptor co-receptors forming heterodimers. Here we report the successful engineering a homozygous mutant strain of diamondback moth ( Plutella xylostella) in which the odorant receptor co-receptor PxOrco was silenced using CRISPR/Cas9. This insect is a globally important crop pest for which novel control methods are urgently required. Behavioral assays demonstrated that PxOrco knockout males exhibited abolished courtship behaviors, inability to mate, and loss of selective preference for P. xylostella's key sex pheromone components. Whilst female mating behavior and fecundity remained unaffected by PxOrco knockout, oviposition response to leaf alcohol, a key cue for normal oviposition behavior, was lost. Electroantennography revealed drastically reduced responses to sex pheromones and plant volatiles in PxOrco-deficient adults but food location by larvae was unaffected. Moreover, expression analysis of PxOrco- deficient pheromone receptors (PRs) indicated varied regulation patterns, with down-regulation observed in several PRs in both sexes. These findings underscore the critical role of PxOrco in regulating multiple olfactory aspects in P. xylostella, including feeding, mating, and host location. Our study identifies the potential of disrupting the Orco gene in this and other pest species to provide novel avenues for future pest control.
Keyword ：
Electroantennography Electroantennography Mating disruption Mating disruption Odorant receptor co-receptors (orco) Odorant receptor co-receptors (orco) Oviposition behavior Oviposition behavior Pheromone Pheromone
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| GB/T 7714 | Chen, Xuanhao , Yao, Shuyuan , Xie, Liangqian et al. Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest [J]. | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 177 . |
| MLA | Chen, Xuanhao et al. "Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest" . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 177 (2025) . |
| APA | Chen, Xuanhao , Yao, Shuyuan , Xie, Liangqian , Li, Jinyang , Xiong, Lei , Yang, Xiaozhen et al. Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 177 . |
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Global climate change is characterized by increased extreme temperatures affecting insects at all trophic levels. Zinc finger proteins (ZFPs) are key regulators of gene expression and cell differentiation in eukaryotes, essential for stress resistance in both animals and plants. Using CRISPR/Cas9 for gene deletion, this study predicted and examined the structure of ZFP320 in the diamondback moth (Plutella xylostella) and investigated its function in temperature stress response through a comprehensive age-stage, two-sex life table analysis. We found ZFP320 encodes a 387 amino acid protein (43 kDa) with no transmembrane domains, featuring a ZnF-C2H2 domain. Quantitative fluorescence analysis showed that ZFP320 expression increased under high temperatures. ZFP320 knockout altered antioxidant gene expression, resulting in higher levels of superoxide dismutase and catalase in mutant strains compared with wild-type strain. Life table analysis revealed that the mutant strains had shorter fecundity and oviposition periods under both normal and high temperatures. Additionally, mutant strains exhibited lower parameters (r, lambda, R0), as well as reduced survival rates and critical thermal maxima. Notably, PxZFP320 plays a crucial role in temperature adaptation, paving the way for future investigations on the significance of ZFPs in P. xylostella's temperature tolerance.
Keyword ：
age-stage two-sex life table age-stage two-sex life table CRISPR/Cas9 CRISPR/Cas9 Plutella xylostella Plutella xylostella temperature adaptability temperature adaptability
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| GB/T 7714 | Miao, Xin , Cao, Fang , Yu, Xiao-Fei et al. A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest [J]. | OPEN BIOLOGY , 2025 , 15 (4) . |
| MLA | Miao, Xin et al. "A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest" . | OPEN BIOLOGY 15 . 4 (2025) . |
| APA | Miao, Xin , Cao, Fang , Yu, Xiao-Fei , Li, Tian-Pu , Su, Hai-Yin , Guo, Jiao et al. A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest . | OPEN BIOLOGY , 2025 , 15 (4) . |
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Fungus gnats, Lycoriella spp. (Diptera: Sciaridae), are significant pests in edible mushroom cultivation worldwide. This study evaluates the effectiveness of 2 soil predatory mites, Stratiolaelaps scimitus (Laelapidae) and Macrocheles glaber (Macrochelidae), in controlling fungus gnats density on the mushroom Agrocybe aegerita. A controlled experiment was performed to examine and compare the effects of individual and combined releases of the 2 mite species on fungus gnats populations and their impact on mushroom yield. Results showed that both mite species significantly reduced fungus gnat populations as compared to untreated controls. However, S. scimitus exhibited superior control, effectively suppressing adult fungus gnats compared to the controls, single release of M. glaber and combined releases (M. glaber + S. scimitus). Mushroom yield was highest in the S. scimitus treatment, with no significant differences observed among other treatments and controls. Interestingly, neither mite species negatively impacted mushroom quality or budding. These findings highlight the significance of S. scimitus as a highly effective biological control agent for suppression of fungus gnats, thereby contributing to sustainable pest management practices in mushroom cultivation.
Keyword ：
Agrocybe aegerita Agrocybe aegerita biological control biological control Laelapidae Laelapidae Macrochelidae Macrochelidae Sciaridae Sciaridae
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| GB/T 7714 | Lan, Qing-Xiu , Wen, Mei-Fang , Lu, Zheng-Hui et al. Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae) [J]. | INSECT SCIENCE , 2025 . |
| MLA | Lan, Qing-Xiu et al. "Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae)" . | INSECT SCIENCE (2025) . |
| APA | Lan, Qing-Xiu , Wen, Mei-Fang , Lu, Zheng-Hui , Ke, Bing-Rong , Fan, Qing-Hai , You, Min-Sheng . Control of fungus gnats Lycoriella sp. in mushroom (Agrocybe aegerita) cultivation with predatory mites Macrocheles glaber (Acari: Macrochelidae) and Stratiolaelaps scimitus (Acari: Laelapidae) . | INSECT SCIENCE , 2025 . |
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Brassica vegetable and oilseed crops are attacked by several different flea beetle species (Chrysomelidae: Alticini). Over the past decades, most research has focused on two Phyllotreta species, Phyllotreta striolata and Phyllotreta cruciferae, which are major pests of oilseed rape in North America. More recently, and especially after the ban of neonicotinoids in the European Union, the cabbage stem flea beetle, Psylliodes chrysocephala, has become greatly important and is now considered to be the major pest of winter oilseed rape in Europe. The major challenges to flea beetle control are the prediction of population dynamics in the field, differential susceptibility to insecticides, and the lack of resistant plant cultivars and other economically viable alternative management strategies. At the same time, many fundamental aspects of flea beetle biology and ecology, which may be relevant for the development of sustainable control strategies, are not well understood. This review focuses on the interactions between flea beetles and plants and summarizes the literature on current management strategies with an emphasis on the potential for biological control in flea beetle management.
Keyword ：
flea beetles flea beetles insect-plant interactions insect-plant interactions integrated pest management integrated pest management pheromone pheromone plant resistance plant resistance
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| GB/T 7714 | Li, Zhenyu , Costamagna, Alejandro Carlos , Beran, Franziska et al. Biology, Ecology, and Management of Flea Beetles in Brassica Crops [J]. | ANNUAL REVIEW OF ENTOMOLOGY , 2024 , 69 : 199-217 . |
| MLA | Li, Zhenyu et al. "Biology, Ecology, and Management of Flea Beetles in Brassica Crops" . | ANNUAL REVIEW OF ENTOMOLOGY 69 (2024) : 199-217 . |
| APA | Li, Zhenyu , Costamagna, Alejandro Carlos , Beran, Franziska , You, Minsheng . Biology, Ecology, and Management of Flea Beetles in Brassica Crops . | ANNUAL REVIEW OF ENTOMOLOGY , 2024 , 69 , 199-217 . |
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Wolbachia bacteria (phylum Proteobacteria) are ubiquitous intracellular parasites of diverse invertebrates. In insects, coevolution has forged mutualistic associations with Wolbachia species, influencing reproduction, immunity, development, pathogen resistance, and overall fitness. However, the impact of Wolbachia on other microbial associates within the insect microbiome, which are crucial for host fitness, remains less explored. The diamondback moth (Plutella xylostella), a major pest of cruciferous vegetables worldwide, harbors the dominant Wolbachia strain plutWB1, known to distort its sex ratio. This study investigated the bacterial community diversity and dynamics across different developmental life stages and Wolbachia infection states in P. xylostella using high-throughput 16S rDNA amplicon sequencing. Proteobacteria and Firmicutes dominated the P. xylostella microbiome regardless of life stage or Wolbachia infection. However, the relative abundance of dominant genera, including an unclassified genus of Enterobacteriaceae, Wolbachia, Carnobacterium, and Delftia tsuruhatensis, displayed significant stage-specific variations. While significant differences in bacterial diversity and composition were observed across life stages, Wolbachia infection had no substantial impact on overall diversity. Nonetheless, relative abundances of specific genera differed between infection states. Notably, Wolbachia exhibited a stable, high relative abundance across all stages and negatively correlated with an unclassified genus of Enterobacteriaceae, Delftia tsuruhatensis, and Carnobacterium. Our findings provide a foundational understanding of the complex interplay between the host, Wolbachia, and the associated microbiome in P. xylostella, paving the way for a deeper understanding of their complex interactions and potential implications for pest control strategies.
Keyword ：
16S rDNA gene sequencing 16S rDNA gene sequencing bacterial communities bacterial communities life stages life stages Plutella xylostella Plutella xylostella Wolbachia Wolbachia
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| GB/T 7714 | Zhu, Xiangyu , Li, Jinyang , He, Ao et al. Developmental Shifts in the Microbiome of a Cosmopolitan Pest: Unraveling the Role of Wolbachia and Dominant Bacteria [J]. | INSECTS , 2024 , 15 (2) . |
| MLA | Zhu, Xiangyu et al. "Developmental Shifts in the Microbiome of a Cosmopolitan Pest: Unraveling the Role of Wolbachia and Dominant Bacteria" . | INSECTS 15 . 2 (2024) . |
| APA | Zhu, Xiangyu , Li, Jinyang , He, Ao , Gurr, Geoff M. , You, Minsheng , You, Shijun . Developmental Shifts in the Microbiome of a Cosmopolitan Pest: Unraveling the Role of Wolbachia and Dominant Bacteria . | INSECTS , 2024 , 15 (2) . |
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The fluctuation in temperature poses a significant challenge for poikilothermic organisms, notably insects, particularly in the context of changing climatic conditions. In insects, temperature adaptation has been driven by polygenes. In addition to genes that directly affect traits (core genes), other genes (peripheral genes) may also play a role in insect temperature adaptation. This study focuses on two peripheral genes, the GRIP and coiled-coil domain containing 2 (GCC2) and karyopherin subunit beta 1 (KPNB1). These genes are differentially expressed at different temperatures in the cosmopolitan pest, Plutella xylostella. GCC2 and KPNB1 in P. xylostella were cloned, and their relative expression patterns were identified. Reduced capacity for thermal adaptation (development, reproduction and response to temperature extremes) in the GCC2-deficient and KPNB1-deficient P. xylostella strains, which were constructed by CRISPR/Cas9 technique. Deletion of the PxGCC2 or PxKPNB1 genes in P. xylostella also had a differential effect on gene expression for many traits including stress resistance, resistance to pesticides, involved in immunity, trehalose metabolism, fatty acid metabolism and so forth. The ability of the moth to adapt to temperature via different pathways is likely to be key to its ability to remain an important pest species under predicted climate change conditions.
Keyword ：
CRISPR/Cas9 system CRISPR/Cas9 system peripheral genes peripheral genes Plutella xylostella Plutella xylostella regulatory network regulatory network temperature adaptability temperature adaptability
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| GB/T 7714 | Lei, Gaoke , Huang, Jieling , Zhou, Huiling et al. Polygenic adaptation of a cosmopolitan pest to a novel thermal environment [J]. | INSECT MOLECULAR BIOLOGY , 2024 , 33 (4) : 387-404 . |
| MLA | Lei, Gaoke et al. "Polygenic adaptation of a cosmopolitan pest to a novel thermal environment" . | INSECT MOLECULAR BIOLOGY 33 . 4 (2024) : 387-404 . |
| APA | Lei, Gaoke , Huang, Jieling , Zhou, Huiling , Chen, Yanting , Song, Jun , Xie, Xuefeng et al. Polygenic adaptation of a cosmopolitan pest to a novel thermal environment . | INSECT MOLECULAR BIOLOGY , 2024 , 33 (4) , 387-404 . |
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Understanding a population's fitness heterogeneity and genetic basis of thermal adaptation is essential for predicting the responses to global warming. We examined the thermotolerance and genetic adaptation of Plutella xylostella to exposure to hot temperatures. The population fitness parameters of the hot- acclimated DBM strains varied in the thermal environments. Using genome scanning and transcription profiling, we find a number of genes potentially involved in thermal adaptation of DBM. Editing two ABCG transporter genes, PxWhite and PxABCG, confirmed their role in altering cuticle permeability and influencing thermal responses. Our results demonstrate that SNP mutations in genes and changes in gene expression can allow DBM to rapidly adapt to thermal environment. ABCG transporter genes play an important role in thermal adaptation of DBM. This work improves our understanding of genetic adaptation mechanisms of insects to thermal stress and our capacity to predict the effects of rising global temperatures on ectotherms.
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| GB/T 7714 | You, Shijun , Lei, Gaoke , Zhou, Huiling et al. Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest [J]. | ISCIENCE , 2024 , 27 (3) . |
| MLA | You, Shijun et al. "Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest" . | ISCIENCE 27 . 3 (2024) . |
| APA | You, Shijun , Lei, Gaoke , Zhou, Huiling , Li, Jianyu , Chen, Shaoping , Huang, Jieling et al. Thermal acclimation uncovers a simple genetic basis of adaptation to high temperature in a cosmopolitan pest . | ISCIENCE , 2024 , 27 (3) . |
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为明确水稻锯齿叶矮缩病毒（rice ragged stunt virus,RRSV）对褐飞虱Nilaparvata lugens生长发育和生殖的影响，分别比较带毒褐飞虱与未带毒褐飞虱的若虫发育历期、成虫寿命、性比、翅型及产卵量，并利用实时荧光定量PCR(real-time quantitative PCR,RT-qPCR)技术分析RRSV侵染后褐飞虱体内卵黄原蛋白（vitellogenin,Vg）与其受体（vitellogenin receptor,VgR）以及海藻糖代谢途径中相关基因表达量的情况。结果显示，带毒褐飞虱的若虫历期显著高于未带毒褐飞虱的若虫历期，分别为17.56 d和15.90 d；带毒褐飞虱的成虫寿命、雌虫比例和短翅比例均高于未带毒褐飞虱的，但两者间均无显著差异；带毒褐飞虱较未带毒褐飞虱单雌日产卵量显著提升，分别为11.26粒和6.45粒；带毒褐飞虱的Vg、VgR和海藻糖转运蛋白基因（trehalose transporter,TRET）表达量均显著高于未带毒褐飞虱的。表明RRSV侵染会显著延长褐飞虱若虫发育历期，同时可能通过上调Vg、VgR以及TRET的表达量以促进褐飞虱生殖。
Keyword ：
水稻锯齿叶矮缩病毒 水稻锯齿叶矮缩病毒 生长发育 生长发育 繁殖力 繁殖力 褐飞虱 褐飞虱
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| GB/T 7714 | 刘健丽 , 李学文 , 欧阳钰莹 et al. 水稻锯齿叶矮缩病毒对褐飞虱生长发育和生殖的影响 [J]. | 植物保护学报 , 2024 , 51 (02) : 377-382 . |
| MLA | 刘健丽 et al. "水稻锯齿叶矮缩病毒对褐飞虱生长发育和生殖的影响" . | 植物保护学报 51 . 02 (2024) : 377-382 . |
| APA | 刘健丽 , 李学文 , 欧阳钰莹 , 郭齐汤 , 尤民生 , 林胜 . 水稻锯齿叶矮缩病毒对褐飞虱生长发育和生殖的影响 . | 植物保护学报 , 2024 , 51 (02) , 377-382 . |
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Plutella xylostella exhibits exceptional reproduction ability, yet the genetic basis underlying the high reproductive capacity remains unknown. Here, we demonstrate that an orphan gene, lushu, which encodes a sperm protein, plays a crucial role in male reproductive success. Lushu is located on the Z chromosome and is prevalent across different P. xylostella populations worldwide. We subsequently generated lushu mutants using transgenic CRISPR/Cas9 system. Knockout of Lushu results in reduced male mating efficiency and accelerated death in adult males. Furthermore, our findings highlight that the deficiency of lushu reduced the transfer of sperms from males to females, potentially resulting in hindered sperm competition. Additionally, the knockout of Lushu results in disrupted gene expression in energy-related pathways and elevated insulin levels in adult males. Our findings reveal that male reproductive performance has evolved through the birth of a newly evolved, lineage-specific gene with enormous potentiality in fecundity success. These insights hold valuable implications for identifying the target for genetic control, particularly in relation to species-specific traits that are pivotal in determining high levels of fecundity. Graphical abstract
Keyword ：
insulin insulin male reproductive fitness male reproductive fitness orphan gene orphan gene sperm competition sperm competition sperm protein sperm protein
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| GB/T 7714 | Zhao, Qian , Zheng, Yahong , Li, Yiying et al. An Orphan Gene Enhances Male Reproductive Success in Plutella xylostella [J]. | MOLECULAR BIOLOGY AND EVOLUTION , 2024 , 41 (7) . |
| MLA | Zhao, Qian et al. "An Orphan Gene Enhances Male Reproductive Success in Plutella xylostella" . | MOLECULAR BIOLOGY AND EVOLUTION 41 . 7 (2024) . |
| APA | Zhao, Qian , Zheng, Yahong , Li, Yiying , Shi, Lingping , Zhang, Jing , Ma, Dongna et al. An Orphan Gene Enhances Male Reproductive Success in Plutella xylostella . | MOLECULAR BIOLOGY AND EVOLUTION , 2024 , 41 (7) . |
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