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学者姓名：杨玮娟
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Abstract ：
本发明公开了一种负载茚虫威的pH‑酶双响应可控缓释材料，其是先采用“一锅法”将农药茚虫威封装在多空隙的骨架结构材料ZIF‑8中，然后通过静电吸附在其颗粒表面依次包覆羧化壳聚糖及壳聚糖而制得。所制得的IDC‑ZIF‑8@CCS/CS对茚虫威的包封率约为70%，将其与红火蚁饵剂混合，可制得用于红火蚁防治的毒饵。本发明所制备的纳米材料基于羧化壳聚糖与壳聚糖的双重包覆材料，具有更好的封装性，能满足农药控制释放用途；纳米材料在雨水中具有高稳定性，有助于减少残留农药对环境的污染；利用其制备的毒饵能够有效减缓红火蚁传毒蚁的死亡速率，有助于延迟因大量工蚁死亡引起的蚁群惊扰和迁移现象，提高传毒效率。
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| GB/T 7714 | 杨玮娟 , 张艺茹 , 汤宝珍 et al. 负载茚虫威的pH-酶双响应可控缓释材料及其制备与应用 : CN202410948419.3[P]. | 2024-07-16 . |
| MLA | 杨玮娟 et al. "负载茚虫威的pH-酶双响应可控缓释材料及其制备与应用" : CN202410948419.3. | 2024-07-16 . |
| APA | 杨玮娟 , 张艺茹 , 汤宝珍 , 侯有明 . 负载茚虫威的pH-酶双响应可控缓释材料及其制备与应用 : CN202410948419.3. | 2024-07-16 . |
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Sulfonamides (SAs) is a class of antibiotics that extensively used for treating infectious diseases in livestock industries and aquaculture. Thus, it is urgent need to obtain the bio-receptor, which has excellent cross-reactivity and specificity to SAs, for developing high-throughput methods for the determination of multiple SAs even all commonly-used SAs, to realize the quick screening/detection of total SAs in animal-derived foods. We herein isolated several SAs-specific cross-reactive aptamers by using a library-immobilized SELEX with multi-SAs parallel selection strategy. Two of the isolated aptamers (Sul-01 and Sul-04) can specifically recognize and bind seven SAs respectively with higher binding affinity and no interference of non-sulfonamide antibiotics, and thus can be applied as bio-receptors for developing high-throughput aptasensors for the quick screening/detection of multiple SAs. By using the mixture of Sul-01 and Sul-04 as bio-receptor, a ratiometric fluorescent aptasensor was created for the quick detection of nine SAs including sulfamethoxydiazine (SMD), sulfapyridine (SPD), sulfaquinoxaline (SQ), sulfathiazole (ST), sulfamonomethoxine (SMM), sulfamerazine (SMR), sulfaguanidine (SG), sulfamethazine (SMZ) and sulfadiazine (SD) with a detection limit (LOD) of 0.10-0.50 mu M, or total of above nine SAs with a LOD of 0.20 mu M. The fluorescent aptasensor was successfully applied to detect each or total of SMD, SPD, SQ, ST, SMM, SMR, SG, SMZ and SD in fish samples with a recovery of 83 %-92 % and a relative standard deviation (RSD, n = 5) < 5 %. This study not only provided several promising bio-receptors for the development of diverse high-throughput aptasensors to achieve the quick screening of multiple SAs residues, but also provided a simple, stable and sensitive method for the quick screening of SMD, SPD, SQ, ST, SMM, SMR, SG, SMZ and SD in seafood.
Keyword ：
Animal-derived foods Animal-derived foods Antibiotics residue Antibiotics residue Aptasensors Aptasensors Seafood Seafood
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| GB/T 7714 | Zhang, Lin , Wu, Siqi , Liu, Junfeng et al. Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood [J]. | TALANTA , 2024 , 277 . |
| MLA | Zhang, Lin et al. "Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood" . | TALANTA 277 (2024) . |
| APA | Zhang, Lin , Wu, Siqi , Liu, Junfeng , Ping, Meiling , Yang, Weijuan , Fu, Fengfu . Isolation of aptamers with excellent cross-reactivity and specificity to sulfonamides towards a ratiometric fluorescent aptasensor for the detection of nine sulfonamides in seafood . | TALANTA , 2024 , 277 . |
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On-site quick detection of acetylcholinesterase (AChE) activity is of great significance for diagnosing nervous diseases, screening drugs, and monitoring poisoning. Herein, we developed a system for sensitively regulating gold nanobipyramid (AuNBP) morphology on a paper-based analytical device (PAD) via an Au+-disproportionation system, and further developed a high-resolution paper-based multicolor sensor (PMS) for both on-site visual detection and laboratory precision quantification of AChE activity using the system as a signal reporter and a dual-enzyme strategy as a receptor. The paper-based Au+ disproportionation-mediated AuNBP morphology system not only has excellent stability, reproducibility, and robust resistibility against reducing substances existing in biological samples but also can effectively suppress the color interference from reagents and greatly improve the sensitivity of regulating AuNBP morphology transformation, making it capable of generating long-term stable and more colorful/clearer color changes corresponding to Au+ concentrations. The developed PMS exhibited eight color changes corresponding to AChE activity within 0.0-1.0 U/L and the color changes could be stably maintained for 30 days, which makes both on-site visual detection and laboratory sensitive quantification possible. The PMS was successfully used to detect AChE activity in serum and erythrocyte samples with a visual detection limit (LOD) of 50 mU/L, a spectrometry LOD of 26 mU/L, and a recovery of 81-102%. The results obtained with the PMS were consistent with those obtained with Ellman's method, verifying its reliability. All of the above features make the PMS a promising assay for the rapid detection of AChE activity in clinical diagnosis. Importantly, the proposed paper-based Au+ disproportionation-mediated AuNBP morphology system can serve as a promising general signal reporter for developing various sensitive multicolor colorimetric sensors for target detection in various fields.
Keyword ：
colorimetricsensor colorimetricsensor enzyme enzyme gold nanocrystals gold nanocrystals nanobipyramids nanobipyramids paper-basedanalytical device paper-basedanalytical device
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| GB/T 7714 | Zhang, Feng , Wang, Zemiao , Fang, Ling et al. Regulating Gold Nanobipyramid Morphology via Au+-Disproportionation System on Paper Toward a Paper-Based Multicolor Sensor for Sensitive Visual Detection of Acetylcholinesterase Activity [J]. | ACS APPLIED NANO MATERIALS , 2024 , 7 (17) : 20933-20941 . |
| MLA | Zhang, Feng et al. "Regulating Gold Nanobipyramid Morphology via Au+-Disproportionation System on Paper Toward a Paper-Based Multicolor Sensor for Sensitive Visual Detection of Acetylcholinesterase Activity" . | ACS APPLIED NANO MATERIALS 7 . 17 (2024) : 20933-20941 . |
| APA | Zhang, Feng , Wang, Zemiao , Fang, Ling , Yang, Weijuan , Wang, Zongwen , Fu, FengFu . Regulating Gold Nanobipyramid Morphology via Au+-Disproportionation System on Paper Toward a Paper-Based Multicolor Sensor for Sensitive Visual Detection of Acetylcholinesterase Activity . | ACS APPLIED NANO MATERIALS , 2024 , 7 (17) , 20933-20941 . |
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Quinolones, a widely used class of antibiotics, present significant environmental and health concerns if they excessively remain in the environment and in food. Aptamers specific to quinolones can be applied as bioreceptors for the detection of quinolone residues in the environment and food. The quinolone family contains dozens of different individuals that share the same core structure coupled with various substituents at six different positions. The diversity and complexity of the substitution sites make it a challenge to choose a set of representative molecules that encompass all the desired sites and preserve the core molecular framework for the screening of quinolone-specific aptamers via systematic evolution of ligands by exponential enrichment (SELEX). To address this challenge, we introduce a novel parallel-series strategy guided by Liebig's law for isolating quinolone-specific cross-reactive aptamers by using the library-immobilized SELEX method. Through this approach, we successfully identified 5 aptamers (Apt.AQ01-Apt.AQ05) with high binding affinity and excellent specificity to 24 different quinolone individuals. Among them, Apt.AQ03 showcased optimal performance with affinities ranging from 0.14 to 1.07 mu M across the comprehensive set of 24 quinolones, exhibiting excellent specificity against nontarget interferents. The binding performance of Apt.AQ03 was further characterized with microscale thermophoresis, circular dichroism spectra, and an exonuclease digestion assay. By using Apt.AQ03 as a bioreceptor, a fluorescence resonance energy transfer (FRET) aptasensor was developed for the detection of 24 quinolones in milk, achieving a remarkable detection limit of 14.5-21.8 ng/mL. This work not only establishes a robust and effective strategy for selecting cross-reactive aptamers applicable to other small-molecule families but also provides high-quality aptamers for developing various high-throughput and reliable methods for the detection of multiple quinolone residues in food.
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| GB/T 7714 | Yang, Weijuan , Luo, Dongdong , Zheng, Siyu et al. Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (21) : 8576-8585 . |
| MLA | Yang, Weijuan et al. "Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy" . | ANALYTICAL CHEMISTRY 96 . 21 (2024) : 8576-8585 . |
| APA | Yang, Weijuan , Luo, Dongdong , Zheng, Siyu , Zhang, Yiru , Wang, Zongwen , Fu, Fengfu . Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (21) , 8576-8585 . |
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On-site visual detection of organophosphorus pesticides (OPs) is crucial for safeguarding environmental and food safety. This study presents a robust, sensitive, and portable paper-based multicolor sensor (PMS) for the on-site detection of OPs. The PMS involves a novel Au+-induced gold nanobipyramids (AuNBPs) morphology transformation reaction within a paper-based analytical device (PAD) coupled with a bienzymatic hydrolysis system. The Au+-induced reaction sensitively regulates the aspect ratio of AuNBPs through Au+ disproportionation, which decreases the length of AuNBPs and simultaneously increases the width, accompanied by distinct multicolor changes. Upon performing in the PAD incorporating easily-prepared AuNBPs-deposited nylon membranes, a metal heater, and an oscillator, the Au+-induced reaction exhibits excellent reaction sensitivity, efficiency, reproducibility, AuNBPs stability, and color uniformity. In the bienzymatic hydrolysis system, acetylcholinesterase (AChE) and choline oxidase hydrolyze acetylcholine to product H2O2, which consumes tris(2-carboxyethyl)phosphine hydrochloride (TCEP), maintaining free Au+ for the disproportionation in the Au+-induced system, while the presence of OPs inhibits AChE activity, preserving TCEP to chelate Au+ and hindering the disproportionation. Under optimized conditions, the PMS exhibited eight dichlorvos concentration-corresponding color changes with a visual detection limit of 30 mu g/L and a spectrometry detection limit of 8.1 mu g/L. It achieved recoveries of 85.4-101.9 % in river water, drinking water, rice, and Chinese cabbage samples with relative standard deviations (n = 5) < 6 %. Thus, this study offers a portable, sensitive, reliable, and practical on-site detection method for OPs. Additionally, the proposed Au+-induced AuNBPs morphology transformation system within the PAD offers promising alternative strategies for developing other AuNBPs-based colorimetric sensors.
Keyword ：
Acetylcholinesterase Acetylcholinesterase Dichlorvos Dichlorvos Gold nanobipyramids Gold nanobipyramids Paper-based analytical device Paper-based analytical device Visual detection Visual detection
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| GB/T 7714 | Zhang, Feng , Ren, Enxi , Wang, Zemiao et al. Portable paper-based multicolor sensor for sensitive on-site detection of organophosphorus pesticides using a high-efficiency Au+-induced gold nanobipyramids aspect ratio regulation strategy [J]. | MICROCHEMICAL JOURNAL , 2024 , 207 . |
| MLA | Zhang, Feng et al. "Portable paper-based multicolor sensor for sensitive on-site detection of organophosphorus pesticides using a high-efficiency Au+-induced gold nanobipyramids aspect ratio regulation strategy" . | MICROCHEMICAL JOURNAL 207 (2024) . |
| APA | Zhang, Feng , Ren, Enxi , Wang, Zemiao , Fang, Ling , Yang, Weijuan , Zhang, Liaoyuan et al. Portable paper-based multicolor sensor for sensitive on-site detection of organophosphorus pesticides using a high-efficiency Au+-induced gold nanobipyramids aspect ratio regulation strategy . | MICROCHEMICAL JOURNAL , 2024 , 207 . |
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On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au(3+)etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au(3+)to A(u+). The presence of OPs inhibits AChE activity, thereby preserving Au3+ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a* parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 mu g/L (visual observation) and limits of detection of 0.8-7.2 mu g/L (colorimeter) and 0.2-3.4 mu g/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.
Keyword ：
Colorimeter Colorimeter Colorimetric biosensor Colorimetric biosensor Gold nanobipyramids-deposited membrane Gold nanobipyramids-deposited membrane Paper-based analytical device Paper-based analytical device The CIELab color space The CIELab color space
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| GB/T 7714 | Zhang, Feng , Gao, Yu , Ren, Enxi et al. Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space [J]. | TALANTA , 2024 , 281 . |
| MLA | Zhang, Feng et al. "Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space" . | TALANTA 281 (2024) . |
| APA | Zhang, Feng , Gao, Yu , Ren, Enxi , Fang, Ling , Yang, Weijuan , Zhang, Liaoyuan et al. Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space . | TALANTA , 2024 , 281 . |
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新质生产力背景下，为了满足国家和区域产业的发展需求，提升服务地方经济的能力，福建农林大学生物制药专业聚焦新型劳动者的培育，着力创新拔尖人才培养模式，构筑高质量的人才自主培养体系。相关专业建设路径包括：以培育新型劳动者为目标，优化人才培养规格；推动“四链”有机衔接，强化校企协同产教融合；以数字化转型为契机，构建教育教学新范式。生物制药专业建设的探索与实践，将为培养匹配新质生产力发展要求的新型劳动者提供值得借鉴的思路和方案。
Keyword ：
人才培养模式 人才培养模式 新质生产力 新质生产力 生物制药专业 生物制药专业
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| GB/T 7714 | 林然 , 廖金英 , 杨玮娟 . 新质生产力视域下生物制药专业人才培养模式的探索与实践 [J]. | 生物化工 , 2024 , 10 (05) : 188-191 . |
| MLA | 林然 et al. "新质生产力视域下生物制药专业人才培养模式的探索与实践" . | 生物化工 10 . 05 (2024) : 188-191 . |
| APA | 林然 , 廖金英 , 杨玮娟 . 新质生产力视域下生物制药专业人才培养模式的探索与实践 . | 生物化工 , 2024 , 10 (05) , 188-191 . |
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The lack of inherent structure -switching functionality and inadequate affinity in aptamers hinders the construction and application of aptasensors. While exonuclease digestion -based strategies have been employed to engineer small -molecule aptamers with the structure -switching functionality and construct dual-exonuclease (exonuclease I and exonuclease III) digestion -based aptasensors, such studies targeting G-quadruplex (G4) aptamers remain scarce. This study focused on the exonuclease digestion and circular dichroism spectroscopy analysis of a G4 quinclorac (QNC) aptamer, Qapt-51, intending to design a functionalized QNC aptamer and a universal signal amplification strategy for constructing folding -based and sensitive dual-exonuclease digestionbased aptasensors, respectively. The exonuclease digestion results demonstrated that QNC-binding Qapt-51 inhibited the dual-exonuclease digestion. Combining the results from the circular dichroism spectroscopy, we further proposed a digestion mechanism for Qapt-51 involving a G4 conformational switch. Inspired by this mechanism, a functionalized Qapt-51 variant (Qapt-45A) was rationally designed to construct a simple and rapid folding -based fluorescence aptasensor. Additionally, we developed an exonuclease III- and phosphorodiamidate morpholino oligomer-assisted fluorescence signal amplification strategy, which can work in the dual-exonuclease digestion system. Leveraging this strategy integrated with the dual-exonuclease digestion of another Qapt-51 variant (AQ), a homogeneous signal -amplified fluorescence aptasensor was constructed to sensitively detect QNC without requiring the functionalized aptamers. These two aptasensors achieved detection limits of 560 ng/ mL and 8.5 ng/mL for QNC. They successfully detected QNC in rice and river water samples with recoveries of 108.6%-87.5%. We hope that the successful development of these two aptasensors can provide valuable insights and references for the broader construction of G4 aptamer-based sensing systems.
Keyword ：
Exonuclease I Exonuclease I Exonuclease III Exonuclease III N -methyl mesoporphyrin IX N -methyl mesoporphyrin IX Phosphorodiamidate morpholino oligomer Phosphorodiamidate morpholino oligomer Recycling amplification Recycling amplification
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| GB/T 7714 | Wang, Zemiao , Zhang, Feng , Fang, Ling et al. Development of folding-based and signal-amplified fluorescence aptasensors using G-quadruplex quinclorac aptamer variants engineered via exonuclease digestion and circular dichroism spectroscopy [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 415 . |
| MLA | Wang, Zemiao et al. "Development of folding-based and signal-amplified fluorescence aptasensors using G-quadruplex quinclorac aptamer variants engineered via exonuclease digestion and circular dichroism spectroscopy" . | SENSORS AND ACTUATORS B-CHEMICAL 415 (2024) . |
| APA | Wang, Zemiao , Zhang, Feng , Fang, Ling , Chen, Fengping , Yang, Weijuan , Wang, Zongwen . Development of folding-based and signal-amplified fluorescence aptasensors using G-quadruplex quinclorac aptamer variants engineered via exonuclease digestion and circular dichroism spectroscopy . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 415 . |
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本发明公开了一种检测乙酰胆碱酯酶活性或有机磷农药的纸基多色比色法，该方法包括以下步骤：将纳米金双锥沉积在尼龙膜上，构建纸基分析装置；将含有Au+的反应溶液加入纸基分析装置中，通过Au+的歧化反应诱导纳米金双锥形貌转化，使得纳米金双锥产生颜色变化和纵向局域表面等离子体共振峰λLLSPR位置变化，从而实现可视化半定量检测和紫外分光光度法定量检测乙酰胆碱酯酶活性或有机磷农药。与现有方法相比，本发明Au+诱导纳米金双锥形貌转化反应能够有效避免实际样品中还原剂的干扰。同时，所构建的纸基分析方法呈现了更好的纳米金双锥储存稳定性、反应重现性以及检测结果的可保存性，还具有高刻蚀效率和高检测灵敏度的优点。
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| GB/T 7714 | 王宗文 , 张峰 , 杨玮娟 . 一种检测乙酰胆碱酯酶活性或有机磷农药的纸基多色比色法 : CN202311586217.0[P]. | 2023-11-24 . |
| MLA | 王宗文 et al. "一种检测乙酰胆碱酯酶活性或有机磷农药的纸基多色比色法" : CN202311586217.0. | 2023-11-24 . |
| APA | 王宗文 , 张峰 , 杨玮娟 . 一种检测乙酰胆碱酯酶活性或有机磷农药的纸基多色比色法 : CN202311586217.0. | 2023-11-24 . |
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Quinclorac is an herbicide widely used in rice production, and its residue in water and soil poses significant threats to aquatic organisms and subsequent crops. We herein isolated a group of aptamers with high binding affinity to quinclorac using library-immobilized SELEX. Notably, one of these aptamers (Apt.Q1) demonstrated excellent specificity and affinity to quinclorac, with dissociation constant (Kd) of 8.7 +/- 2.4 mu M and no interference from structurally similar pesticides. Molecular dynamics simulations identified the G-rich loop in Apt.Q1 as the potential binding domain, and circular dichroism analysis revealed the conformational transition from hairpin to G-triplex structure upon quinclorac interaction. Moreover, a one-step, label-free colorimetric biosensor was developed based on quinclorac-mediated displacement of Cy7 dye and Apt.Q1 for detecting quinclorac within 1 min with a detection limit of 237 nM and RSD < 5%. Assessment of quinclorac residues in soil and river water using the biosensor demonstrated recoveries of 89-92%. The study not only provided a promising bioreceptor for developing various quinclorac biosensors but also provided a cost-effective tool for rapidly screening quinclorac in environment. Specifically, the investigation of the recognition mechanism between Apt. Q1 and quinclorac lays a theoretical foundation for designing more sensitive and reliable sensing platforms for quinclorac detection.
Keyword ：
Aptamer Aptamer Colorimetric sensor Colorimetric sensor Dye-displacement Dye-displacement Quinclorac Quinclorac SELEX SELEX
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| GB/T 7714 | Yang, Weijuan , Luo, Dongdong , Xiao, Yufan et al. Isolation and characterization of the quinclorac-specific aptamer toward a colorimetric sensor for the visual detection of quinclorac in water and soil [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
| MLA | Yang, Weijuan et al. "Isolation and characterization of the quinclorac-specific aptamer toward a colorimetric sensor for the visual detection of quinclorac in water and soil" . | SENSORS AND ACTUATORS B-CHEMICAL 393 (2023) . |
| APA | Yang, Weijuan , Luo, Dongdong , Xiao, Yufan , Zheng, Siyu , Wang, Zongwen , Fu, Fengfu . Isolation and characterization of the quinclorac-specific aptamer toward a colorimetric sensor for the visual detection of quinclorac in water and soil . | SENSORS AND ACTUATORS B-CHEMICAL , 2023 , 393 . |
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