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学者姓名：云英子
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Abstract ：
In eukaryotes, the retromer complex plays a crucial role in the sorting and retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN). Despite its importance, the molecular details of this intracellular transport process remain unclear. Here, we have identified a Golgi-associated retrograde protein (GARP) complex as a mediator of vesicle transport that facilitates the recruitment of the retromer complex to the TGN to exert its functions. The GARP complex is mainly localized in the TGN where it interacts with the retromer complex. This interaction is evolutionarily conserved across species. Furthermore, we identified FgKex2 and FgSnc1 as cargo proteins in the GARP/retromer-mediated recycling pathway. Loss of GARP or retromer results in a complete missorting of FgKex2 and FgSnc1 into the vacuolar degradation pathway, which affects the growth, development, biogenesis of toxisomes and pathogenicity of Fusarium graminearum. In summary, we demonstrate for the first time that GARP promotes the recruitment of retromer from endosomes to the TGN, thereby establishing a GARP/retromer transport pathway that coordinates the recycling of cargo proteins FgKex2 and FgSnc1. This process is essential for maintaining sustained growth and development and significantly contributes to the pathogenicity of F. graminearum.
Keyword ：
cargo sorting cargo sorting fungal development fungal development Fusarium graminearum Fusarium graminearum GARP GARP retromer retromer
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| GB/T 7714 | Long, Yunfei , Chen, Xin , Chen, Jia et al. Golgi-associated retrograde protein (GARP) complex recruits retromer to trans-Golgi network for FgKex2 and FgSnc1 recycling, necessary for the development and pathogenicity of Fusarium graminearum [J]. | NEW PHYTOLOGIST , 2025 , 246 (2) : 666-688 . |
| MLA | Long, Yunfei et al. "Golgi-associated retrograde protein (GARP) complex recruits retromer to trans-Golgi network for FgKex2 and FgSnc1 recycling, necessary for the development and pathogenicity of Fusarium graminearum" . | NEW PHYTOLOGIST 246 . 2 (2025) : 666-688 . |
| APA | Long, Yunfei , Chen, Xin , Chen, Jia , Zhang, Haoran , Lin, Ying , Cheng, Shuyuan et al. Golgi-associated retrograde protein (GARP) complex recruits retromer to trans-Golgi network for FgKex2 and FgSnc1 recycling, necessary for the development and pathogenicity of Fusarium graminearum . | NEW PHYTOLOGIST , 2025 , 246 (2) , 666-688 . |
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香蕉枯萎病(Fusarium wilt)是由尖镰孢古巴专化型(Fusarium oxysporum f. sp.cubense,Foc)引起的一种土传病害，严重威胁着香蕉生产。生物防治是香蕉枯萎病综合治理体系中的重要组成部分。本研究以同一生境中的2种二倍体香蕉(斑叶蕉、野蕉)和2种三倍体香蕉(天宝矮蕉、矮杆大蕉)为研究对象，通过高通量测序分析其根部内生细菌群落的多样性，发现二倍体植株的内生细菌群落多样性高于三倍体植株；这两种类型香蕉根部内生细菌群落结构也存在一定的差异，二倍体植株中芽孢杆菌属(Bacillus)、慢生根瘤菌属(Bradyrhizobium)等重要生防微生物的物种相对丰度高于三倍体植株。从二倍体香蕉(紫苞芭蕉、斑叶蕉)中分离筛选到14株对Foc具有拮抗效果的内生细菌，盆栽试验结果表明其中2株贝莱斯芽孢杆菌(Bacillus velezensis) ZB-1和Z-7灌根处理均能有效减轻由Foc引起的香蕉枯萎病的发病程度，其脂肽提取物可影响Foc分生孢子及菌丝的形态建成，导致Foc分生孢子产量分别下降了99.02%和98.67%,并破坏菌丝生物膜系统及抑制脂质代谢。本研究表明二倍体香蕉富含有益生防菌群，为开发基于微生物调控的香蕉枯萎病绿色防控技术体系提供了重要的理论依据和菌种资源。
Keyword ：
内生细菌 内生细菌 拮抗内生菌 拮抗内生菌 根部 根部 香蕉枯萎病 香蕉枯萎病
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| GB/T 7714 | 林亚琪 , 杨俊杰 , 卢松茂 et al. 香蕉根部内生细菌群落多样性及香蕉枯萎病拮抗细菌筛选 [J]. | 植物病理学报 , 2025 , 55 (04) : 978-990 . |
| MLA | 林亚琪 et al. "香蕉根部内生细菌群落多样性及香蕉枯萎病拮抗细菌筛选" . | 植物病理学报 55 . 04 (2025) : 978-990 . |
| APA | 林亚琪 , 杨俊杰 , 卢松茂 , 吕培涛 , 郑文辉 , 云英子 . 香蕉根部内生细菌群落多样性及香蕉枯萎病拮抗细菌筛选 . | 植物病理学报 , 2025 , 55 (04) , 978-990 . |
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由香蕉枯萎病菌4号生理小种(Fusarium oxysporum f. sp.cubense tropical race 4,Foc TR4)引起的香蕉枯萎病是全球香蕉产业面临的重大土传病害，其土壤中的分生孢子和厚垣孢子是主要侵染源。构巢曲霉(Aspergillus nidulans)C2H2型锌指转录因子FlbC是调控分生孢子发育的关键因子。本研究在Foc TR4中鉴定到FlbC的同源基因FocFlbC,利用原生质体介导的遗传转化技术构建了其敲除突变体(ΔFocFlbC)和回补菌株，并分析了该蛋白的亚细胞定位和生物学功能。结果显示，FocFlbC定位于菌丝和分生孢子的细胞核。与野生型相比，ΔFocFlbC突变体在麦芽糖培养基上的菌丝生长速率显著降低，但在其他碳源培养基上无明显差异；该突变体在不同碳源培养基上的产孢量均显著降低，其中在麦芽糖培养基上野生型与突变体的产孢量比率最高，显著高于其他碳源培养基。虽然FocFlbC缺失突变对Foc TR4生物量累积和分生孢子萌发无显著影响，但与野生型相比，该突变体表现出：细胞壁和盐胁迫耐受性降低；α-淀粉酶、滤纸酶及β-1,4-D-葡聚糖酶活性下降，且相应水解酶基因的表达水平下调；以及致病力减弱。上述表型缺陷均可被回补菌株恢复。综上，FocFlbC不仅调控Foc TR4在麦芽糖培养基上的菌丝生长与产孢，还参与调控细胞壁完整性、盐胁迫响应、水解酶合成以及致病力等多个重要生物学过程，为深入揭示Foc TR4生长发育和致病分子机制奠定了理论基础。
Keyword ：
C2H2型锌指结构域 C2H2型锌指结构域 水解酶活性 水解酶活性 碳源利用 碳源利用 胁迫响应 胁迫响应 致病性 致病性 转录因子 转录因子 香蕉枯萎病菌 香蕉枯萎病菌
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| GB/T 7714 | 卢松茂 , 林秀香 , 林亚琪 et al. 香蕉枯萎病菌C2H2型锌指转录因子FocFlbC的功能研究 [J]. | 植物病理学报 , 2025 , 55 (04) : 649-662 . |
| MLA | 卢松茂 et al. "香蕉枯萎病菌C2H2型锌指转录因子FocFlbC的功能研究" . | 植物病理学报 55 . 04 (2025) : 649-662 . |
| APA | 卢松茂 , 林秀香 , 林亚琪 , 林晓兰 , 杨帅 , 郑文辉 et al. 香蕉枯萎病菌C2H2型锌指转录因子FocFlbC的功能研究 . | 植物病理学报 , 2025 , 55 (04) , 649-662 . |
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The signal peptidase complex (SPC) is a crucial membrane enzyme complex involved in the process of protein secretion and maturation in both prokaryotic and eukaryotic cells. SPC is responsible for the cleavage of N-terminal signal sequences from nascent proteins, a sequence of amino acids that directs the newly synthesized protein to the secretory pathway. The yeast SPC is composed of four subunits: Spc1, Spc2, Spc3, and Sec11. To understand how SPC functions in the fungal plant pathogen, we identified the SPC component gene MoSPC2 and characterized its functions in M. oryzae. Through measuring the colony diameter of the Delta Mospc2 mutant and control strains on culture medium plates, quantifying conidia production, observing conidial morphology, and assessing pathogenicity on rice and barley plants, we found that MoSpc2 contributes to fungal growth, asexual development, and pathogenicity. Since host-derived reactive oxygen species (ROS) are crucial for rice to defend against M. oryzae, we further investigated the role of MoSpc2 in ROS modulation. Our results indicate that MoSpc2 plays a pivotal role in suppressing the accumulation of ROS and regulating the activities of extracellular peroxidases and laccases. Notably, MoSpc2 mediates the accumulation and secretion of the effector protein MoSlp1. Furthermore, using affinity purification, we discovered MoSpc2-interacting proteins and identified potential SPC interactors. These candidates provide a foundation for future mechanistic studies aimed at elucidating their functional roles in SPC complex assembly and pathogenic regulation. Our results highlight the significance of the SPC component gene MoSPC2 involvement in fungal development and pathogenicity and widen our understanding of the connections between the SPC and fungal pathogenesis.
Keyword ：
Fungal development Fungal development Magnaporthe oryzae Magnaporthe oryzae MoSpc2 MoSpc2 Pathogenicity Pathogenicity Signal peptidase Signal peptidase
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| GB/T 7714 | Tang, Wei , Wang, Min , Bao, Ying et al. The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae [J]. | RICE , 2025 , 18 (1) . |
| MLA | Tang, Wei et al. "The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae" . | RICE 18 . 1 (2025) . |
| APA | Tang, Wei , Wang, Min , Bao, Ying , Chen, Weiquan , Jiang, Yanfang , Chen, Meilian et al. The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae . | RICE , 2025 , 18 (1) . |
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【目的】研究尖孢镰刀菌古巴专化型（Foc）铜伴侣蛋白FocCCS1的生物学功能，为香蕉枯萎病的绿色防控提供参考。【方法】利用MEGA-X软件构建FocCCS1基因的遗传进化树；通过基因敲除技术从Foc 4号热带生理小种（FocTR4）中敲除FocCCS1,构建基因敲除突变体和回补菌株，研究该基因对Foc生长、产孢、环境胁迫响应、致病性、菌丝穿透和黏附及镰刀菌酸合成等方面的调控作用。【结果】FocCCS1基因长度为879 bp,编码292个氨基酸，包含重金属ATP酶（HMA）和Sod＿Cu结构域，其与轮枝镰刀菌FvCCS1亲缘关系最近。表型分析结果显示，FocCCS1参与调控Foc菌丝的生长、分生孢子的产生和萌发、对Cu
Keyword ：
Cu Cu 基因敲除 基因敲除 致病性 致病性 营养生长 营养生长 超氧化物歧化酶铜伴侣 超氧化物歧化酶铜伴侣
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| GB/T 7714 | 邓火兵 , 尤海霞 , 黄梅梅 et al. 尖孢镰刀菌古巴专化型铜伴侣蛋白FocCCS1的功能分析 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (06) : 732-741 . |
| MLA | 邓火兵 et al. "尖孢镰刀菌古巴专化型铜伴侣蛋白FocCCS1的功能分析" . | 福建农林大学学报(自然科学版) 53 . 06 (2024) : 732-741 . |
| APA | 邓火兵 , 尤海霞 , 黄梅梅 , 尹凡 , 杨金涛 , 云英子 . 尖孢镰刀菌古巴专化型铜伴侣蛋白FocCCS1的功能分析 . | 福建农林大学学报(自然科学版) , 2024 , 53 (06) , 732-741 . |
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SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) facilitate docking and fusion of vesicles with their target membranes, playing a crucial role in vesicle trafficking and exocytosis. However, the spatial assembly and roles of plasma membrane (PM)-associated SNAREs in phytopathogen development and pathogenicity are not clearly understood. In this study, we analysed the roles and molecular mechanisms of PM-associated SNARE complexes in the banana Fusarium wilt fungus Fusarium oxysporum f. sp. cubense tropical race 4 (FocTR4). Our findings demonstrate that FocSso1 is important for the fungal growth, conidiation, host penetration and colonization. Mechanistically, FocSso1 regulates protein secretion by mediating vesicle docking and fusion with the PM and hyphal apex. Interestingly, a FocSso1-FocSec9-FocSnc1 complex was observed to assemble not only at the fungal PM but also on the growing hyphal apex, facilitating exocytosis. FocSso2, a paralogue of FocSso1, was also found to form a ternary SNARE complex with FocSec9 and FocSnc1, but it mainly localizes to the PM in old hyphae. The functional analysis of this protein demonstrated that it is dispensable for the fungal growth but necessary for host penetration and colonization. The other subunits, FocSec9 and FocSnc1, are involved in the fungal development and facilitate host penetration. Furthermore, FocSso1 and FocSnc1 are functionally interdependent, as loss of FocSso1 leads to mis-sorting and degradation of FocSnc1 in the vacuole and vice versa. Overall, this study provides insight into the formation of two spatially and functionally distinct PM SNARE complexes and their involvement in vesicle exocytosis to regulate development and pathogenicity of FocTR4.
Keyword ：
banana Fusarium wilt fungus banana Fusarium wilt fungus exocytosis exocytosis pathogenicity pathogenicity SNARE SNARE vesicle vesicle
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| GB/T 7714 | Fang, Zhenyu , Zhao, Qiwen , Yang, Shiyu et al. Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense [J]. | MOLECULAR PLANT PATHOLOGY , 2024 , 25 (3) . |
| MLA | Fang, Zhenyu et al. "Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense" . | MOLECULAR PLANT PATHOLOGY 25 . 3 (2024) . |
| APA | Fang, Zhenyu , Zhao, Qiwen , Yang, Shiyu , Cai, Yan , Fang, Wenqin , Abubakar, Yakubu Saddeeq et al. Two distinct SNARE complexes mediate vesicle fusion with the plasma membrane to ensure effective development and pathogenesis of Fusarium oxysporum f. sp. cubense . | MOLECULAR PLANT PATHOLOGY , 2024 , 25 (3) . |
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The conidia produced by Fusarium oxysporum f. sp. cubense (Foc), the causative agent of Fusarium Wilt of Banana (FWB), play central roles in the disease cycle, as the pathogen lacks a sexual reproduction process. Until now, the molecular regulation network of asexual sporogenesis has not been clearly understood in Foc. Herein, we identified and functionally characterized thirteen (13) putative sporulation-responsive genes in Foc, namely FocmedA(a), FocmedA(b), abaA-L, FocflbA, FocflbB, FocflbC, FocflbD, FocstuA, FocveA, FocvelB, wetA-L, FocfluG and Foclae1. We demonstrated that FocmedA(a), abaA-L, wetA-L, FocflbA, FocflbD, FocstuA, FocveA and Foclae1 mediate conidiophore formation, whereas FocmedA(a) and abaA-L are important for phialide formation and conidiophore formation. The expression level of abaA-L was significantly decreased in the Delta FocmedA(a) mutant, and yeast one-hybrid and ChIP-qPCR analyses further confirmed that FocMedA(a) could bind to the promoter of abaA-L during micro- and macroconidiation. Moreover, the transcript abundance of the wetA-L gene was significantly reduced in the Delta abaA-L mutant, and it not only was found to function as an activator of micro- and macroconidium formation but also served as a repressor of chlamydospore production. In addition, the deletions of FocflbB, FocflbC, FocstuA and Foclae1 resulted in increased chlamydosporulation, whereas FocflbD and FocvelB gene deletions reduced chlamydosporulation. Furthermore, FocflbC, FocflbD, Foclae1 and FocmedA(a) were found to be important regulators for pathogenicity and fusaric acid synthesis in Foc. The present study therefore advances our understanding of the regulation pathways of the asexual development and functional interdependence of sporulation-responsive genes in Foc.
Keyword ：
asexual reproduction asexual reproduction Fusarium oxysporum f. sp. cubense Fusarium oxysporum f. sp. cubense genes genes sporogenesis regulation sporogenesis regulation
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| GB/T 7714 | Lu, Songmao , Deng, Huobing , Lin, Yaqi et al. A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense [J]. | JOURNAL OF FUNGI , 2024 , 10 (1) . |
| MLA | Lu, Songmao et al. "A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense" . | JOURNAL OF FUNGI 10 . 1 (2024) . |
| APA | Lu, Songmao , Deng, Huobing , Lin, Yaqi , Huang, Meimei , You, Haixia , Zhang, Yan et al. A Network of Sporogenesis-Responsive Genes Regulates the Growth, Asexual Sporogenesis, Pathogenesis and Fusaric Acid Production of Fusarium oxysporum f. sp. cubense . | JOURNAL OF FUNGI , 2024 , 10 (1) . |
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Most fresh bananas belong to the Cavendish and Gros Michel subgroups. Here, we report chromosome-scale genome assemblies of Cavendish (1.48 Gb) and Gros Michel (1.33 Gb), defining three subgenomes, Ban, Dh and Ze, with Musa acuminata ssp. banksii, malaccensis and zebrina as their major ancestral contributors, respectively. The insertion of repeat sequences in the Fusarium oxysporum f. sp. cubense (Foc) tropical race 4 RGA2 (resistance gene analog 2) promoter was identified in most diploid and triploid bananas. We found that the receptor-like protein (RLP) locus, including Foc race 1-resistant genes, is absent in the Gros Michel Ze subgenome. We identified two NAP (NAC-like, activated by apetala3/pistillata) transcription factor homologs specifically and highly expressed in fruit that directly bind to the promoters of many fruit ripening genes and may be key regulators of fruit ripening. Our genome data should facilitate the breeding and super-domestication of bananas. Chromosome-scale genome assemblies of triploid Cavendish and Gros Michel reveal the banana cultivars' origins, disease resistance and fruit ripening mechanism.
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| GB/T 7714 | Li, Xiuxiu , Yu, Sheng , Cheng, Zhihao et al. Origin and evolution of the triploid cultivated banana genome [J]. | NATURE GENETICS , 2023 , 56 (1) . |
| MLA | Li, Xiuxiu et al. "Origin and evolution of the triploid cultivated banana genome" . | NATURE GENETICS 56 . 1 (2023) . |
| APA | Li, Xiuxiu , Yu, Sheng , Cheng, Zhihao , Chang, Xiaojun , Yun, Yingzi , Jiang, Mengwei et al. Origin and evolution of the triploid cultivated banana genome . | NATURE GENETICS , 2023 , 56 (1) . |
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<正>垂花蝎尾蕉(Heliconia rostrata Ruiz & Pav.),又名金嘴蝎尾蕉、金嘴赫蕉、垂花火鸟蕉，是蝎尾蕉科(Heliconiaceae)蝎尾蕉属 (Heliconias) 多年生草本植物，原产美洲热带地区阿根廷至秘鲁一带，1970年从马来西亚引入我国，主要栽种于华南地区~([1])。垂花蝎尾蕉的花序轴排列方式为二列状，形状酷似蝎尾，花形奇特，花色艳丽，花期5—10月，广泛运用于园林景观、园艺盆栽，是一种名贵垂吊切花植物。
Keyword ：
Bipolaris setariae Bipolaris setariae Heliconia rostrata Heliconia rostrata leaf spot leaf spot
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| GB/T 7714 | 杨俊杰 , 黄桂兰 , 林亚琪 et al. 垂花蝎尾蕉平脐蠕孢叶斑病病原菌的鉴定 [J]. | 植物病理学报 , 2023 , 53 (06) : 1232-1235 . |
| MLA | 杨俊杰 et al. "垂花蝎尾蕉平脐蠕孢叶斑病病原菌的鉴定" . | 植物病理学报 53 . 06 (2023) : 1232-1235 . |
| APA | 杨俊杰 , 黄桂兰 , 林亚琪 , 邓火兵 , 应瑞赟 , 云英子 . 垂花蝎尾蕉平脐蠕孢叶斑病病原菌的鉴定 . | 植物病理学报 , 2023 , 53 (06) , 1232-1235 . |
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科学施用杀菌剂是植物病害综合治理的重要措施之一，然而由于杀菌剂的长期使用，病菌抗药性问题逐渐加重，严重影响药剂的防治效果和使用寿命。近年来，随着分子生物学技术的快速发展，人们对杀菌剂抗性机制有了更深入的理解，并开发出了病菌抗药基因型快速检测的方法。本文总结了植物病原真菌对苯并咪唑类杀菌剂(BZD)、肌球蛋白合成抑制剂、甾醇脱甲基抑制剂(DMI)、QoI类抑制剂、琥珀酸脱氢酶抑制剂(SDHI)和二甲酰亚胺类杀菌剂(DC)的抗药性现状与抗性机制。在此基础上，介绍了聚合酶链反应(PCR)、限制性片段长度多态性(RFLP)、等位基因特异性PCR和环介导等温扩增(LAMP)技术在杀菌剂抗性快速检测方面的研究进展。此外，对抗药性治理对策进行了讨论和展望。
Keyword ：
抗性检测 抗性检测 抗性治理对策 抗性治理对策 杀菌剂抗性机制 杀菌剂抗性机制 植物病原真菌 植物病原真菌
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| GB/T 7714 | 吴小美 , 王海霞 , 云英子 et al. 植物病原真菌对杀菌剂抗性的研究进展 [J]. | 植物保护 , 2023 , 49 (05) : 243-259 . |
| MLA | 吴小美 et al. "植物病原真菌对杀菌剂抗性的研究进展" . | 植物保护 49 . 05 (2023) : 243-259 . |
| APA | 吴小美 , 王海霞 , 云英子 , 马忠华 . 植物病原真菌对杀菌剂抗性的研究进展 . | 植物保护 , 2023 , 49 (05) , 243-259 . |
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