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学者姓名：顾晓军
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Abstract ：
传统植物化学保护实践课中出现学生对农药种类繁多识别困难的畏难情绪、对农药生物测定设计性实验项目的连续失败受挫感，以及对运用植保无人机（UAV）等智慧农药施用手段仅能观摩无法动手实践抱有遗憾等问题，我们有效利用大学生的争强好胜心理，在实践教学中引入不同的挑战模式。农药种类多采用“限时分组PK式挑战”强化学生记忆；设计性实验项目耗时长采用“分段闯关式挑战”增强学生的自我肯定；高端农业智慧设备学习采用“人机大赛PK式挑战”拉大差距提高体验感。挑战模式教学法的课后问卷调查结果表明，大部分学生的实践学习内动力被充分激发出来，主动求知意识、表达交流能力及团队合作意识均得到明显提高。
Keyword ：
人机大赛 人机大赛 挑战教学 挑战教学 植物化学保护实践教学 植物化学保护实践教学 闯关挑战 闯关挑战 限时分组PK 限时分组PK
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| GB/T 7714 | 林美珍 , 顾晓军 , 黄劲飞 et al. 挑战教学模式在植物化学保护实践教学中的应用 [J]. | 智慧农业导刊 , 2025 , 5 (09) : 151-154,158 . |
| MLA | 林美珍 et al. "挑战教学模式在植物化学保护实践教学中的应用" . | 智慧农业导刊 5 . 09 (2025) : 151-154,158 . |
| APA | 林美珍 , 顾晓军 , 黄劲飞 , 肖顺 . 挑战教学模式在植物化学保护实践教学中的应用 . | 智慧农业导刊 , 2025 , 5 (09) , 151-154,158 . |
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Abstract ：
农药为保障世界粮食安全做出了巨大贡献，但也曾对人类健康及生态环境造成了一些负面影响。全社会都需要树立正确的农药观，包括正确的农药安全观、农药使用观与农药管理观3个方面。具体观点是，当前农药安全性已经达到可接受水平，但是其负面影响不会完全消失；农药的使用与管理应兼顾经济效益、社会效益和生态效益的统一。必须遵循的原则包括历史与现实分开、时代的局限与农药的缺陷分开、事实真相与认识误区分开、农药正确使用与错误使用甚至非法使用分开、科学管理与绿色壁垒分开。在此基础上，通过权衡利弊和扬长避短正确使用农药，以及稳中求进和先立后破有效管理农药，农药一定能为有效保障人类粮食安全做出更大贡献。
Keyword ：
使用 使用 农药观 农药观 安全 安全 管理 管理 综述 综述
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| GB/T 7714 | 顾晓军 , 林美珍 , 兰亦全 et al. 浅议如何树立正确的农药观 [J]. | 现代农药 , 2025 , 24 (03) : 50-54 . |
| MLA | 顾晓军 et al. "浅议如何树立正确的农药观" . | 现代农药 24 . 03 (2025) : 50-54 . |
| APA | 顾晓军 , 林美珍 , 兰亦全 , 黄劲飞 , 田素芬 . 浅议如何树立正确的农药观 . | 现代农药 , 2025 , 24 (03) , 50-54 . |
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Targeting juvenile hormone esterase (JHE) is an emerging strategy to combat the broadly resistant pest, Plutella xylostella; this study employed transcriptomics to investigate the sublethal effects of the JHE inhibitor OTFP, revealing a non-monotonic dose response characterized by stronger transcriptional changes at lower concentrations, resulting in low mortality, prolonged pupation time, and increased pupal weight. The results from the Differentially Expressed Genes (DEGs) analysis revealed that the core effect of OTFP is the persistent perturbation of the "insect hormone biosynthesis" pathway and altered expression of components of the JH/20E axis; to cope with this stress, the larvae exhibited a dual defense associated with compensatory upregulation of JH-degrading enzyme genes to attempt to restore hormone homeostasis, and the activation of a broad-spectrum detoxification network to clear the compound. More critically, the developmental delay resulting from endocrine disruption KEGG-enriched growth-related pathways (amino-acid and central-carbon metabolism; ribosome biogenesis; aminoacyl-tRNA biosynthesis), consistent with a growth-permissive milieu during prolonged feeding. This study therefore elucidates a novel integrative regulatory network that links endocrine disruption, detoxification, and compensatory growth, revealing a complex physiological trade-off strategy in this pest that sacrifices developmental tempo for survival.
Keyword ：
insect hormone pathway insect hormone pathway juvenile hormone esterase juvenile hormone esterase OTFP OTFP Plutella xylostella Plutella xylostella RNA-seq RNA-seq
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| GB/T 7714 | Wang, Yingbo , Wang, Xiying , Lin, Yubin et al. Transcriptomic Analysis of the Diamondback Moth Under Exposure to the Juvenile Hormone Esterase (JHE) Inhibitor 3-Octylthio-1,1,1-trifluoro-2-propanone (OTFP) [J]. | INSECTS , 2025 , 16 (11) . |
| MLA | Wang, Yingbo et al. "Transcriptomic Analysis of the Diamondback Moth Under Exposure to the Juvenile Hormone Esterase (JHE) Inhibitor 3-Octylthio-1,1,1-trifluoro-2-propanone (OTFP)" . | INSECTS 16 . 11 (2025) . |
| APA | Wang, Yingbo , Wang, Xiying , Lin, Yubin , Zheng, Shun , Qiu, Jianrong , Gao, Jinheng et al. Transcriptomic Analysis of the Diamondback Moth Under Exposure to the Juvenile Hormone Esterase (JHE) Inhibitor 3-Octylthio-1,1,1-trifluoro-2-propanone (OTFP) . | INSECTS , 2025 , 16 (11) . |
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Thrips hawaiiensis (Morgan) (Thysanoptera: Thripidae) is a sap-sucking pest that seriously damages several crops and reduces their economic value. Exposure to low concentrations of insecticides may have a sublethal effect on surviving insects. In order to provide a reference for the rational application of emamectin benzoate, its sublethal effects on the development and reproduction of T. hawaiiensis were evaluated. Pupal development time was significantly shorter in T. hawaiiensis treated with sublethal concentrations of emamectin benzoate (LC10 and LC20) than in control. Female adult longevity and female total longevity were significantly longer following LC20 treatment than in the control and LC10 treatment groups. Nevertheless, male adult longevity and male total longevity were significantly shorter in the LC10 treatment group than in the control and LC20 treatment groups. The sublethal concentration of emamectin benzoate (LC20) significantly shortened the preadult stages and the mean generation. Meanwhile, it significantly increased the finite rate of increase, intrinsic rate of increase, and net reproductive rate. The fecundity was significantly higher after LC20 treatment than after LC10 and control treatments. Compared with the control group, the LC10 and LC20 groups of T. hawaiiensis adults showed a significantly higher expression of the vitellogenin (Vg) and vitellogenin receptor (VgR) genes, which played a key role in increasing their fecundity. These findings suggest that short-term exposure to sublethal concentrations of emamectin benzoate may lead to a resurgence and secondary outbreak of T. hawaiiensis infestation. The results have practical applications for the management of this important and noxious pest.
Keyword ：
life table life table sublethal effect sublethal effect Thrips hawaiiensis Thrips hawaiiensis vitellogenin vitellogenin vitellogenin receptor vitellogenin receptor
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| GB/T 7714 | Chen, Yi-Xin , Tian, Hou-Jun , Lin, Shuo et al. Sublethal effects of emamectin benzoate on development, reproduction, and vitellogenin and vitellogenin receptor gene expression in Thrips hawaiiensis (Thysanoptera: Thripidae) [J]. | JOURNAL OF INSECT SCIENCE , 2023 , 23 (3) . |
| MLA | Chen, Yi-Xin et al. "Sublethal effects of emamectin benzoate on development, reproduction, and vitellogenin and vitellogenin receptor gene expression in Thrips hawaiiensis (Thysanoptera: Thripidae)" . | JOURNAL OF INSECT SCIENCE 23 . 3 (2023) . |
| APA | Chen, Yi-Xin , Tian, Hou-Jun , Lin, Shuo , Yu, Yun , Xie, Lian-Cheng , Li, Heng et al. Sublethal effects of emamectin benzoate on development, reproduction, and vitellogenin and vitellogenin receptor gene expression in Thrips hawaiiensis (Thysanoptera: Thripidae) . | JOURNAL OF INSECT SCIENCE , 2023 , 23 (3) . |
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【目的】本研究旨在为阐明小菜蛾Plutella xylostella体温在其防治中的应用价值提供资料。【方法】在不同人工气候箱内温度(环境温度)下，测定小菜蛾2, 3和4龄幼虫的体温，建立各龄幼虫体温(y)与环境温度(x)的关系方程；同时测定了不同环境温度下不同浓度阿维菌素、毒死蜱、氟虫腈和高效氯氰菊酯分别处理后小菜蛾3龄幼虫在不同处理时间的体温。【结果】小菜蛾2, 3和4龄幼虫体温(y)与环境温度(x)关系方程分别为y=0.95x+1.19(r=0.9463),y=0.95x+1.18(r=0.9988),以及y=0.93x+1.45(r=0.9989),等温点分别为22.16℃, 21.40℃和21.41℃。在气候箱温度设定为15℃或40℃时，4种农药都对小菜蛾3龄幼虫体温无影响；而其他温度条件下，农药处理都可能改变小菜蛾3龄幼虫体温。对于阿维菌素，25℃下2, 4和8 mg/L处理12 h, 2和4 mg/L处理24 h, 0.5, 2, 4和8 mg/L处理36 h以及0.5, 1, 2和8 mg/L处理48 h时3龄幼虫体温均显著高于对照，8 mg/L阿维菌素处理24 h时3龄幼虫体温显著低于对照；30℃下0.5 mg/L处理24 h及1 mg/L处理36 h 3龄幼虫体温显著低于对照，1 mg/L处理48 h和各浓度处理60 h时3龄幼虫体温均显著高于对照；35℃下只有1和8 mg/L处理48 h时3龄幼虫体温显著低于对照。对于毒死蜱，20℃下50, 200和800 mg/L处理24 h, 100, 400和800 mg/L处理36 h时3龄幼虫体温都显著低于对照；25℃下100和200 mg/L处理12 h, 800 mg/L处理24 h, 100, 200和800 mg/L处理60 h时3龄幼虫体温均显著低于对照，而50, 100, 200和400 mg/L处理24 h, 100和200 mg/L处理36 h及100和400 mg/L处理48 h时3龄幼虫体温均显著高于对照；30℃下只有800 mg/L处理24 h时3龄幼虫体温显著低于对照，50, 100, 200和800 mg/L处理60 h时3龄幼虫体温显著高于对照。对于氟虫腈，20℃下只有0.5 mg/L处理36 h时3龄幼虫体温显著低于对照；25℃下4 mg/L处理12 h和各浓度处理60 h时3龄幼虫体温显著低于对照，0.5 mg/L处理24 h以及0.25, 1和2 mg/L处理48 h时3龄幼虫体温均显著高于对照；30℃下0.25和0.5 mg/L处理12 h, 0.25和2 mg/L处理24 h, 4 mg/L处理48 h以及2 mg/L处理60 h时3龄幼虫体温显著低于对照；35℃下只有0.25和0.5 mg/L处理60 h时3龄幼虫体温显著高于对照。对于高效氯氰菊酯，20℃下2和8 g/L处理36 h, 4和8 g/L处理48 h时3龄幼虫体温显著高于对照；25℃下2, 4和8 g/L处理12 h时3龄幼虫体温均显著低于对照，0.5, 4和8 g/L处理24 h, 1, 4和8 g/L处理36 h以及1, 2和4 g/L处理60 h时3龄幼虫体温均显著高于对照；30℃下0.5和1 g/L浓度处理12 h, 0.5, 1, 4和8 g/L处理24 h以及1, 2和8 g/L处理60 h时3龄幼虫体温都显著低于对照。【结论】小菜蛾幼虫自律性体温调节能力低；阿维菌素、毒死蜱、氟虫腈或高效氯氰菊酯处理影响小菜蛾3龄幼虫的体温，影响形式随农药种类和浓度，环境温度及处理时间不同而不同。本研究拓宽了农药毒理学及害虫防治研究内容。
Keyword ：
体温 体温 农药 农药 小菜蛾 小菜蛾 毒力 毒力 等温点 等温点
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| GB/T 7714 | 田素芬 , 李志华 , 李元涛 et al. 四种农药对小菜蛾体温的影响 [J]. | 昆虫学报 , 2022 , 65 (09) : 1115-1126 . |
| MLA | 田素芬 et al. "四种农药对小菜蛾体温的影响" . | 昆虫学报 65 . 09 (2022) : 1115-1126 . |
| APA | 田素芬 , 李志华 , 李元涛 , 林硕 , 陈艺欣 , 田厚军 et al. 四种农药对小菜蛾体温的影响 . | 昆虫学报 , 2022 , 65 (09) , 1115-1126 . |
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【目的】本研究旨在构建用于小菜蛾Plutella xylostella蛹期特异表达基因Br-Z2/3 dsRNA合成的体外原核表达系统,研究RNAi抑制Br-Z2/3基因对小菜蛾Br-Z2/3和细胞凋亡基因表达及化蛹的影响。【方法】构建小菜蛾L4440-Br-Z2/3重组载体,转化大肠杆菌Escherichia coli HT115感受态细胞,经IPTG诱导大量获得Br-Z2/3 dsRNA,显微注射Br-Z2/3 dsRNA至小菜蛾4龄幼虫进行RNAi; qPCR检测干扰Br-Z2/3基因12和24 h后小菜蛾4龄幼虫Br-Z2/3及其下游细胞凋亡基因reaper,caspase-9和Gadd45g的表达量;观察并统计Br-Z2/3 RNAi后小菜蛾4龄幼虫的化蛹率、平均化蛹时间、蛹畸形率和幼虫死亡率。【结果】成功实现了Br-Z2/3 dsRNA的原核表达。qPCR结果表明,RNAi干扰Br-Z2/3后,小菜蛾4龄幼虫Br-Z2/3基因和相关联的细胞凋亡基因reaper表达量显著下降,但caspase-9和Gadd45g表达量显著上升。注射Br-Z2/3 dsRNA的处理组,化蛹率显著低于对照组,且化蛹高峰期推迟,幼虫死亡率显著高于对照组且畸形蛹率增加。【结论】本研究成功构建了用于小菜蛾Br-Z2/3基因dsRNA合成的体外原核表达系统,利用显微注射法对Br-Z2/3进行RNA干扰,证明Br-Z2/3是调控小菜蛾化蛹的关键基因。
Keyword ：
Br-Z2/3 Br-Z2/3 dsRNA dsRNA RNA干扰 RNA干扰 化蛹 化蛹 小菜蛾 小菜蛾 显微注射 显微注射 细胞凋亡基因 细胞凋亡基因
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| GB/T 7714 | 胡晓汉 , 田素芬 , 李亚勍 et al. RNAi抑制小菜蛾Br-Z2/3基因对下游细胞凋亡基因表达及化蛹的影响 [J]. | 昆虫学报 , 2021 , 64 (02) : 141-148 . |
| MLA | 胡晓汉 et al. "RNAi抑制小菜蛾Br-Z2/3基因对下游细胞凋亡基因表达及化蛹的影响" . | 昆虫学报 64 . 02 (2021) : 141-148 . |
| APA | 胡晓汉 , 田素芬 , 李亚勍 , 林硕 , 陈艺欣 , 魏辉 et al. RNAi抑制小菜蛾Br-Z2/3基因对下游细胞凋亡基因表达及化蛹的影响 . | 昆虫学报 , 2021 , 64 (02) , 141-148 . |
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【目的】活化蛋白激酶C受体1(receptor for activated C kinase 1, RACK1)参与了多细胞生物体中重要的信号传导,调节生物体的生长发育。本研究旨在克隆小菜蛾Plutella xylostella RACK1基因PxyRACK1,调查其表达模式,明确其对小菜蛾化蛹的影响及其机制。【方法】克隆PxyRACK1的全长cDNA,进行生物信息学分析,并利用qPCR检测其在小菜蛾各发育阶段(卵、1-4龄幼虫、预蛹、蛹和成虫)的表达;通过dsPxyRACK1显微注射小菜蛾4龄幼虫进行RNA干扰,并检测RNAi后PxyRACK1及蛹期特异表达基因PxyBr-Z2/3的表达量;统计死亡率、化蛹率、平均化蛹时间以及蛹重。【结果】小菜蛾PxyRACK1(GenBank登录号:MW160739)序列全长为1 148 bp,开放阅读框长960 bp,编码319个氨基酸,具有7个WD40重复序列,每个WD40重复序列包含39～42个氨基酸。PxyRACK1在小菜蛾各发育阶段均有表达,其中4龄第2天幼虫中表达量最高,2日龄蛹中表达量最低。显微注射dsPxyRACK1 24 h后,处理组4龄幼虫中PxyRACK1和PxyBr-Z2/3表达量较对照组(dsGFP注射组)的分别显著降低了36.26%和83.46%,并且显微注射dsPxyRACK1导致试虫死亡率上升、化蛹推迟、化蛹率降低以及蛹重减轻。【结论】结果说明PxyRACK1参与调控小菜蛾蛹期变态发育。本研究为进一步阐明小菜蛾变态发育的信号调控通路及开发新型昆虫生长调节剂提供了思路。
Keyword ：
PxyBr-Z2/3 PxyBr-Z2/3 PxyRACK1 PxyRACK1 RNA干扰 RNA干扰 小菜蛾 小菜蛾 显微注射 显微注射
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| GB/T 7714 | 胡晓汉 , 田素芬 , 林硕 et al. 小菜蛾活化蛋白激酶C受体1基因PxyRACK1在变态发育调控中的作用 [J]. | 昆虫学报 , 2021 , 64 (08) : 887-896 . |
| MLA | 胡晓汉 et al. "小菜蛾活化蛋白激酶C受体1基因PxyRACK1在变态发育调控中的作用" . | 昆虫学报 64 . 08 (2021) : 887-896 . |
| APA | 胡晓汉 , 田素芬 , 林硕 , 陈艺欣 , 魏辉 , 顾晓军 et al. 小菜蛾活化蛋白激酶C受体1基因PxyRACK1在变态发育调控中的作用 . | 昆虫学报 , 2021 , 64 (08) , 887-896 . |
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The diamondback moth, Plutella xylostella (L.), is a worldwide insect pest of cruciferous crops. Although insecticides have long been used for its control, diamondback moth rapidly evolves resistance to almost any insecticide. In insects, juvenile hormone (JH) is critically involved in almost all biological processes. The correct activity of JH depends on the precise regulation of its titer, and juvenile hormone esterase (JHE) is the key regulator. Thus, JH and JHE have become important targets for new insecticide development. Trifluoromethyl ketones are specific JHE inhibitors, among which 3-octylthio-1,1,1-trifluoropropan-2-one (OTFP) has the highest activity. The interaction effects between pretreatment with or combination of OTFP and the insecticides diafenthiuron, indoxacarb, and Bacillus thuringiensis (Bt) were investigated in diamondback moth larvae to determine OTFP's potential as an insecticide synergist. In third-instar larvae, both pretreatment and combination treatment with OTFP decreased or antagonized the toxicities of diafenthiuron, indoxacarb, and Bt at all set concentrations. In fourth-instar larvae, combination treatment with OTFP decreased or antagonized the toxicities of diafenthiuron and indoxacarb at all set concentrations. However, it increased or synergized the toxicity of Bt at lower concentrations despite the limited effect at higher concentrations. Our results indicated that the effect of OTFP on the toxicities of insecticides varied with the type and concentration, larval stage, and treatment method. These findings contribute to the better use of OTFP in diamondback moth control.
Keyword ：
3-octylthio-1,1,1-trifluoropropan-2-one 3-octylthio-1,1,1-trifluoropropan-2-one Bacillus thuringiensis (Bt) Bacillus thuringiensis (Bt) diafenthiuron diafenthiuron indoxacarb indoxacarb Plutella xylostella Plutella xylostella
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| GB/T 7714 | Huang, Jingfei , Tian, Sufen , Ren, Ke et al. Effect of Treatment With 3-Octylthio-1,1,1-Trifluoropropan-2-One in the Diamondback Moth (Lepidoptera: Plutellidae) to the Toxicity of Diafenthiuron, Indoxacarb, and Bacillus thuringiensis [J]. | JOURNAL OF ECONOMIC ENTOMOLOGY , 2020 , 113 (3) : 1419-1425 . |
| MLA | Huang, Jingfei et al. "Effect of Treatment With 3-Octylthio-1,1,1-Trifluoropropan-2-One in the Diamondback Moth (Lepidoptera: Plutellidae) to the Toxicity of Diafenthiuron, Indoxacarb, and Bacillus thuringiensis" . | JOURNAL OF ECONOMIC ENTOMOLOGY 113 . 3 (2020) : 1419-1425 . |
| APA | Huang, Jingfei , Tian, Sufen , Ren, Ke , Chen, Yong , Lin, Shuo , Chen, Yixin et al. Effect of Treatment With 3-Octylthio-1,1,1-Trifluoropropan-2-One in the Diamondback Moth (Lepidoptera: Plutellidae) to the Toxicity of Diafenthiuron, Indoxacarb, and Bacillus thuringiensis . | JOURNAL OF ECONOMIC ENTOMOLOGY , 2020 , 113 (3) , 1419-1425 . |
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The diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), is a widespread and destructive pest of cruciferous crops. New strategies for controlling it are needed because it is rapidly developing resistance to conventional pesticides. In insects, transcription factors (TFs) including broad-complex (Br-C) are thought to be useful for insecticide development because they are able to regulate the transcription of functional genes involved in responses to external stimuli including insecticides. In the present study, we cloned and sequenced the open reading frames (ORFs) of three BTB-ZF encoding genes from the diamondback moth deposited in the National Center for Biotechnology Information (NCBI) database under accessions MG753773, MG288674, and MG753772. The lengths of these ORFs were 1,680, 1,428, and 1,647 bp, respectively. The phylogenetic analysis based on the predicted amino acid sequences of ZF domains showed that MG753773 and MG288674 belonged to Z2/Z3 and Z7 of Br-C while MG753772 belonged to Ttk types. In the agreement, the highest expression level of MG753773 occurred during the prepupal stage, MG288674 and MG753772 were expressed during all stages and peaked in the adult and egg stages, respectively. RNA interference silencing of MG753773 in the late third instar larvae significantly decreased survival and pupation of the insects. With precocene II, transcription of MG753773 increased (4x) in the fourth instar larva 24 hr later; 48 hr later the rate of prepupation and pupation was significantly higher. These findings will contribute to the development of new regulators of the growth and development for diamondback moth control.
Keyword ：
broad-complex isoform 2/3 broad-complex isoform 2/3 expression profiling expression profiling metamorphosis metamorphosis open reading frame open reading frame Plutella xylostella Plutella xylostella precocene II precocene II RNA interference RNA interference
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| GB/T 7714 | Huang, Jingfei , Fang, Ling , Wang, Shuai et al. Molecular cloning, expression profiling, and functional analysis of a broad-complex isoform 2/3 (Br-Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.) [J]. | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY , 2019 , 101 (2) . |
| MLA | Huang, Jingfei et al. "Molecular cloning, expression profiling, and functional analysis of a broad-complex isoform 2/3 (Br-Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.)" . | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 101 . 2 (2019) . |
| APA | Huang, Jingfei , Fang, Ling , Wang, Shuai , Liu, Xiang , Chen, Yong , Chen, Yixin et al. Molecular cloning, expression profiling, and functional analysis of a broad-complex isoform 2/3 (Br-Z2/Z3) transcription factor in the diamondback moth, Plutella xylostella (L.) . | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY , 2019 , 101 (2) . |
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Insect neuropeptides in the pyrokinin/pheromone biosynthesis-activating neuropeptide (PBAN) family are actively involved in many essential endocrinal functions and serve as potential targets in the search for novel insect control agents. Here, we dissect the nervous system of larval, pupal, and adult Plutella xylostella (L.) (Lepidoptera: Plutellidae) and describe the ganglion morphology and localization of PBAN during different insect developmental stages. Our results show that the central nervous system (CNS) of this species consists of four types of ganglia: cerebral ganglia (brain), subesophageal ganglion (SEG), thoracic ganglia, and abdominal ganglia. A two-lobed brain is connected to the reniform SEG with a nerve cord in larvae and prepupae, whereas in the late pupae and adults, the brain and SEG are fused, forming a brain-SEG complex. The larvae and prepupae have eight abdominal ganglia each, whereas the late pupae and adults each have four abdominal ganglia. Furthermore, all life stages of P. xylostella had similar patterns of PBAN immunoreactivity in the CNS, and the accumulation of PBAN was similar during all life stages except in adult males. PBAN immunoreactive signals were observed in the brain and SEG, and fluorescence signals originating in the SEG extended the entire length of the ventral nerve cord, ending in the terminal abdominal ganglia. Our results provide morphological data that inform the development and evolution of the CNS. In addition, they indicate that the nervous system contains PBAN, which could be used to control P. xylostella populations.
Keyword ：
central nervous system central nervous system immunocytochemistry immunocytochemistry neuropeptide neuropeptide PBAN PBAN Plutella xylostella Plutella xylostella
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| GB/T 7714 | Chen, Yong , Liu, Yuyan , Tian, Houjun et al. Distribution of Pheromone Biosynthesis-Activating Neuropeptide in the Central Nervous System of Plutella xylostella (Lepidoptera: Plutellidae) [J]. | JOURNAL OF ECONOMIC ENTOMOLOGY , 2019 , 112 (6) : 2638-2648 . |
| MLA | Chen, Yong et al. "Distribution of Pheromone Biosynthesis-Activating Neuropeptide in the Central Nervous System of Plutella xylostella (Lepidoptera: Plutellidae)" . | JOURNAL OF ECONOMIC ENTOMOLOGY 112 . 6 (2019) : 2638-2648 . |
| APA | Chen, Yong , Liu, Yuyan , Tian, Houjun , Chen, Yixin , Lin, Shuo , Mao, Qianzhuo et al. Distribution of Pheromone Biosynthesis-Activating Neuropeptide in the Central Nervous System of Plutella xylostella (Lepidoptera: Plutellidae) . | JOURNAL OF ECONOMIC ENTOMOLOGY , 2019 , 112 (6) , 2638-2648 . |
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