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学者姓名：侯有明
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Abstract ：
Serpins (serine protease inhibitors) constitute a superfamily of proteins with functional diversity and unusual conformational flexibility. In insects, serpins act as multiple inhibitors, by forming inactive acyl-enzyme complexes, in regulating Sp & auml;tzles activation, phenoloxidases (POs) activity, and other cytokines. In this study, we present the cloning and characterization of Octodonta nipae serpin2 (OnSPN2), a 415 residues protein homologous to Tenebrio molitor 42Dd-like. Notably, OnSPN2 features an arginine residue (R364) at the P1 position, and additional arginine residues (R362, R367) at the P3 and P3 ' positions, respectively which is crucial for protease inhibition. Immunohistochemistry (IHC) and Western blot analyses revealed that OnSPN2 is primarily synthesized in plasmatocytes and then released into the plasma to exert its function. RNA interference results indicated that OnSPN2 knockdown may depress serine protease in melanization and remarkably increase the transcript level of Attacin in hemolymph, but its messenger RNA levels were not changed upon immune induction. Reciprocal co-immunoprecipitation assay results confirmed that OnSPN2 binds to OnPPAF1 and OnSP8, indicating its role as a negative regulator in the PO and AMP pathway. Intriguingly, several cathepsin-L isoforms were identified in the OnSPN2 immunoprecipitated samples. The cathepsin-L inhibition assays and protein-protein docking results, identified cathepsin-L as a potential target of OnSPN2. These results indicate that OnSPN2 is produced as an intracellular resident and additionally is associated with the PO and AMP pathway. OnSPN2 represents a multiple defense tool that may provide multiple antiproteolytic functions.
Keyword ：
cathepsin-L cathepsin-L Octodonta nipae Octodonta nipae phenoloxidase cascade phenoloxidase cascade serine protease serine protease serpin serpin
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| GB/T 7714 | Zhang, Huajian , Xu, Jiawei , Chen, Mintao et al. The OnSPN2 from the nipa palm hispid beetle Octodonta nipae is a multipurpose defense tool against proteases from different peptidase families [J]. | INSECT SCIENCE , 2025 . |
| MLA | Zhang, Huajian et al. "The OnSPN2 from the nipa palm hispid beetle Octodonta nipae is a multipurpose defense tool against proteases from different peptidase families" . | INSECT SCIENCE (2025) . |
| APA | Zhang, Huajian , Xu, Jiawei , Chen, Mintao , Yin, Jiawei , Hou, Youming , Tang, Baozhen . The OnSPN2 from the nipa palm hispid beetle Octodonta nipae is a multipurpose defense tool against proteases from different peptidase families . | INSECT SCIENCE , 2025 . |
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Insects have highly developed olfactory systems in which cytochrome P450s (CYPs) were involved as odor-degrading enzymes throughout the olfactory recognition of odor compounds by insects to avoid continuous stimulation of signaling molecules and thus damage to the olfactory nervous. To understand whether the highly expressed CYPs in the antennae play an olfactory function in Solenopsis invicta worker, in this study, we find six highly expressed antennal CYPs from the transcriptome of S. invicta. Multiple sequence alignment and phylogenetic analysis divided them into two families: the CYP3 family (SinvCYP6K1, SinvCYP6K1-1) and the CYP4 family (SinvCYP4C1, SinvCYP4C1-1, SinvCYP4C1-2, SinvCYP4V2). The expression patterns of these six CYPs were analyzed by RT-qPCR, which revealed that SinvCYP6K1 and SinvCYP4V2 were only highly expressed in the antennae of adult workers. The expression of SinvCYP6K1 and SinvCYP4V2 in workers was markedly diminished after feeding with dsRNA. The electroantennography (EAG) assay demonstrated that the silencing of either SinvCYP6K1 or SinvCYP4V2 resulted in a notable reduction in the EAG response of workers to 2-ethyl-3,6(5)-dimethylpyrazine (EDMP). Furthermore, the trajectory behavior assay showed that the worker's range and speed of movement in response to EDMP significant decreased after the silencing of SinvCYP6K1 and SinvCYP4V2. The findings indicated that both SinvCYP6K1 and SinvCYP4V2 were implicated in the recognition of EDMP by S. invicta.
Keyword ：
alarm pheromone alarm pheromone cytochrome P450s cytochrome P450s electroantennography electroantennography red imported fire ant red imported fire ant RNA interference RNA interference
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| GB/T 7714 | Jiang, Xinyi , Shen, Jiacheng , Lin, Peng et al. High Antennal Expression of CYP6K1 and CYP4V2 Participate in the Recognition of Alarm Pheromones by Solenopsis invicta Buren [J]. | INSECTS , 2025 , 16 (1) . |
| MLA | Jiang, Xinyi et al. "High Antennal Expression of CYP6K1 and CYP4V2 Participate in the Recognition of Alarm Pheromones by Solenopsis invicta Buren" . | INSECTS 16 . 1 (2025) . |
| APA | Jiang, Xinyi , Shen, Jiacheng , Lin, Peng , Hou, Youming . High Antennal Expression of CYP6K1 and CYP4V2 Participate in the Recognition of Alarm Pheromones by Solenopsis invicta Buren . | INSECTS , 2025 , 16 (1) . |
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Research on the interactions between herbivorous insects and plants, facilitated by insect secretions, has increasingly emphasized species with chewing mandibles over time. However, the molecular mechanisms underlying the interaction between Spodoptera frugiperda and plants remain poorly understood. In this study, we identified a protein disulfide isomerase (SfPDI) from the salivary glands of S. frugiperda that regulates the interaction between S. frugiperda and plants. We found that SfPDI is highly expressed in the salivary glands of S. frugiperda and is secreted into plants as a secretory protein. The RNAi revealed that SfPDI contributes to the growth and development of S. frugiperda on host plants, while its overexpression in tobacco induces necrosis in tobacco leaves and triggers a burst of reactive oxygen species (ROS). Differentially expressed genes suggested that SfPDI may suppresses the expression of plant JA by positively regulating MYC2 and TIFYs and negatively regulating WRKYs. Notably, SfPDI may modulate these high expression of receptors (NB-LRR, GL-RLK, and RLK) lead to hypersensitive response (HR) cell death and the accumulation of lignification of plant. This study provides a foundation for further exploring insect-plant interaction mechanisms and a theoretical basis for developing insect-resistant germplasm and environmentally friendly pest control strategies.
Keyword ：
HR HR Insect-plant interaction Insect-plant interaction Plant defense Plant defense Salivary protein Salivary protein S. frugiperda S. frugiperda
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| GB/T 7714 | Cai, Xiang-Yun , Tang, Hua-Tao , Wang, Yu-Zhou et al. Salivary effector SfPDI modulates plant defense responses to enhance foraging efficiency of Spodoptera frugiperda [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 308 . |
| MLA | Cai, Xiang-Yun et al. "Salivary effector SfPDI modulates plant defense responses to enhance foraging efficiency of Spodoptera frugiperda" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 308 (2025) . |
| APA | Cai, Xiang-Yun , Tang, Hua-Tao , Wang, Yu-Zhou , Ul Haq, Inzamam , Wang, Jin-Da , Hou, You-Ming . Salivary effector SfPDI modulates plant defense responses to enhance foraging efficiency of Spodoptera frugiperda . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 308 . |
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The tomato leafminer, Tuta absoluta, is an invasive pest that poses a significant threat to Solanaceae crops, including tomatoes, potatoes, and eggplants. Its ability to adapt to various environmental conditions, especially cold stress, allows it to survive in regions with fluctuating temperatures. In this study, the molecular response of T. absoluta adults to rapid cold hardening (RCH) under gradually decreasing non-lethal temperatures was investigated. Using RNA-seq, 197 upregulated genes and 626 downregulated genes were identified in response to cold hardening. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses revealed that upregulated genes were primarily associated with the cell cycle and cell division, including key genes such as CDC25A, BUB1, ORC2, and PlK1, as well as metal ion transporters like unc-93. Downregulated genes were found to be involved in metabolism and oxidative phosphorylation, including COX4, ATPase_I, and ATP-synt_ab. Weighted gene coexpression network analysis (WGCNA) indicated a positive correlation between cell cycle-related genes and RCH, while metabolic processes, including fatty acid metabolism, were negatively correlated with cold hardening. These findings provide new insights into the transcriptional mechanisms of insect responses to cold stress, and highlight potential targets for environmentally sustainable pest management strategies.
Keyword ：
cell cycle cell cycle cold stress adaptation cold stress adaptation metabolic process metabolic process RNA-seq RNA-seq Tuta absoluta Tuta absoluta
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| GB/T 7714 | Zhao, Yan , Ullah, Farman , Shi, Jiayi et al. Transcriptomic Responses to Rapid Cold Hardening in Tomato Leafminer Adults: Implications for Tomato Leafminer Management [J]. | AGRONOMY-BASEL , 2025 , 15 (3) . |
| MLA | Zhao, Yan et al. "Transcriptomic Responses to Rapid Cold Hardening in Tomato Leafminer Adults: Implications for Tomato Leafminer Management" . | AGRONOMY-BASEL 15 . 3 (2025) . |
| APA | Zhao, Yan , Ullah, Farman , Shi, Jiayi , Hu, Yingjie , Jiang, Yuyang , Hou, Youming . Transcriptomic Responses to Rapid Cold Hardening in Tomato Leafminer Adults: Implications for Tomato Leafminer Management . | AGRONOMY-BASEL , 2025 , 15 (3) . |
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BACKGROUNDThe red imported fire ant (RIFA, Solenopsis invicta), a highly destructive invasive pest, has rapidly spread through human trade, posing significant threats to agricultural and forest ecosystems. Due to its preference for high-fat and high-protein foods, ham sausage is commonly used as bait to monitor RIFA populations in invaded areas. However, the presence of volatile organic compounds (VOCs) in such baits may affect their effectiveness because VOCs can act as either attractants or repellents. Identifying VOCs that specifically attract RIFA is essential to improve bait efficacy.RESULTSThis study aimed to identify attractant compounds within bait VOCs for RIFA, leveraging the highly-expressed antennal odorant-binding protein 1 (OBP1) and reverse chemical ecology approach. Additionally, we examined the effects of mixtures of these attractants on RIFA behavior. Our findings revealed that anethole, 1S-(-)-beta-pinene, and beta-caryophyllene individually attracted RIFA at 0.1 mu g/mu L. Notably, a combination of anethole and 1S-(-)-beta-pinene enhanced behavioral activity more than individual compounds, suggesting synergistic effects. Conversely, the addition of beta-caryophyllene to anethole significantly reduced RIFA activity. These results provide a theoretical basis for developing behavioral regulators targeting RIFA.CONCLUSIONThis study demonstrates that the integration of OBP-based in vitro assays with computational simulations can effectively identify behaviorally active compounds for RIFA. Additionally, it clarifies the optimal ratios of active VOCs in baits, offering valuable theoretical guidance for enhancing RIFA population monitoring efforts. (c) 2025 Society of Chemical Industry.
Keyword ：
insect olfactory insect olfactory invasive pest invasive pest odorant-binding protein odorant-binding protein reverse chemical ecology reverse chemical ecology social insect social insect
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| GB/T 7714 | Shen, Jiacheng , Wu, Sheng-Yen , Lin, Peng et al. Identification and optimization of volatile organic compounds to enhance bait attractiveness for red imported fire ants (Solenopsis invicta Buren) [J]. | PEST MANAGEMENT SCIENCE , 2025 , 81 (6) : 3240-3249 . |
| MLA | Shen, Jiacheng et al. "Identification and optimization of volatile organic compounds to enhance bait attractiveness for red imported fire ants (Solenopsis invicta Buren)" . | PEST MANAGEMENT SCIENCE 81 . 6 (2025) : 3240-3249 . |
| APA | Shen, Jiacheng , Wu, Sheng-Yen , Lin, Peng , Jiang, Xinyi , Hou, Youming . Identification and optimization of volatile organic compounds to enhance bait attractiveness for red imported fire ants (Solenopsis invicta Buren) . | PEST MANAGEMENT SCIENCE , 2025 , 81 (6) , 3240-3249 . |
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The tomato leaf miner, Tuta absoluta, is a pest threatening global tomato production. This pest's adaptability and resistance to chemical insecticides have necessitated integrated pest management (IPM) strategies prioritizing sustainable alternatives. This review explores the role of biological control agents (BCAs) in managing T. absoluta populations, emphasizing the integration of floral resources to enhance their efficacy. Predatory mirids such as Macrolophus pygmaeus and Nesidiocoris tenuis and parasitoids such as N. artynes and Trichogramma spp. are pivotal in pest suppression; however, their performance depends on nutritional and habitat support. Floral resources provide essential sugars and proteins, improving the longevity, fecundity, and predation efficiency of these BCAs. This review synthesizes case studies highlighting the benefits of selected flowering plants, such as Lobularia maritima and Fagopyrum esculentum, in supporting predator and parasitoid populations while minimizing advantages to T. absoluta. Mechanisms such as nectar quality, floral accessibility, and spatial-temporal resource availability are explored in detail. Additionally, the challenges of selective floral attraction, microbial impacts on nectar composition, and the unintended support of non-target organisms are discussed. This review proposes targeted floral management strategies to optimize BCA performance within IPM systems by integrating ecological and chemical insights. This approach offers a pathway toward reducing chemical pesticide reliance, fostering sustainable agriculture, and mitigating the economic impacts of T. absoluta infestations.
Keyword ：
biological control agents biological control agents floral resource management floral resource management integrated pest management integrated pest management sustainable agriculture sustainable agriculture T. absoluta T. absoluta
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| GB/T 7714 | Hyder, Moazam , Ul Haq, Inzamam , Younas, Muhammad et al. Floral Resource Integration: Enhancing Biocontrol of Tuta absoluta Within Sustainable IPM Frameworks [J]. | PLANTS-BASEL , 2025 , 14 (3) . |
| MLA | Hyder, Moazam et al. "Floral Resource Integration: Enhancing Biocontrol of Tuta absoluta Within Sustainable IPM Frameworks" . | PLANTS-BASEL 14 . 3 (2025) . |
| APA | Hyder, Moazam , Ul Haq, Inzamam , Younas, Muhammad , Ghafar, Muhammad Adeel , Akhtar, Muhammad Rehan , Ahmed, Zubair et al. Floral Resource Integration: Enhancing Biocontrol of Tuta absoluta Within Sustainable IPM Frameworks . | PLANTS-BASEL , 2025 , 14 (3) . |
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Tuta absoluta is a significant agricultural pest primarily affecting Solanaceae plants, resulting in substantial economic losses in agriculture. Insect saliva is an intermediary between insects and plants, playing a crucial role in modulating host adaptability and plant defense. This study analyzed the adaptive differences of T. absoluta on four plants using the two-sex life table method. Results indicated that the host adaptability of T. absoluta to tobacco is worse than its adaptability to the other three varieties of tomatoes. The salivary gland transcriptome analysis and signal peptide prediction revealed that Trypsin, B5 V51-1498, and Ta74 were highly expressed in the salivary glands of T. absoluta subjected to tobacco treatment and exhibit the characteristics of secretory proteins, alongside significant feeding selection differences. Our findings elucidate the adaptive strategies of T. absoluta larvae on various Solanaceae plants and offer new insights into the salivary protein-mediated plant defense processes.
Keyword ：
host adaptation host adaptation interaction interaction salivary protein salivary protein transcriptome transcriptome Tuta absoluta Tuta absoluta
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| GB/T 7714 | Li, Xiujie , Cai, Xiangyun , Shang, Luohua et al. Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (5) : 2814-2829 . |
| MLA | Li, Xiujie et al. "Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 5 (2025) : 2814-2829 . |
| APA | Li, Xiujie , Cai, Xiangyun , Shang, Luohua , Wang, Yuzhou , Haq, Inzamam Ul , Wang, Jinda et al. Adaptability Analysis of Tuta absoluta to Different Hosts and Related Salivary Genes Identification . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (5) , 2814-2829 . |
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Plant secondary metabolites are crucial in affecting the interactions between insect herbivores and entomoviruses. However, there is limited knowledge regarding the impact of such metabolites on the susceptibility of insect herbivores to entomoviruses. In this study, we adopted the allicin, caterpillars (Spodoptera exigua) and nucleopolyhedrovirus (SeMNPV) as a system, and found that allicin significantly increased the mortality of S. exigua larvae infected with SeMNPV by 36.03-59.45% when infected with the virus at a concentration of 2.12 x 10(3) OBmL(-1). Furthermore, NPV-infected larvae together treated with allicin inhibited the growth and development of larvae, comparing to individual NPV-infected larvae. Notably, we observed a significant enrichment of differentially expressed genes involved in the cytochrome P450-mediated metabolism pathway between the NPV-infected and allicin combined with NPV-treated groups. The silencing of CYP340AA1 through RNA interference significantly increased the mortality of larvae infected with SeMNPV. This investigation indicates that allicin might be a potential candidate for improving the performance of the NPV against insect herbivores and identifies that CYP340AA1 gene is important in this process.
Keyword ：
Allicin Allicin RNA interference RNA interference RNA-Seq RNA-Seq Spodoptera exigua Spodoptera exigua Spodoptera exigua multiple nucleopolyhedrovirus Spodoptera exigua multiple nucleopolyhedrovirus
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| GB/T 7714 | Wang, Jin-Yan , Jiang, Jie-Xian , Fan, Neng-Neng et al. Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus [J]. | JOURNAL OF PEST SCIENCE , 2025 , 98 (2) : 747-757 . |
| MLA | Wang, Jin-Yan et al. "Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus" . | JOURNAL OF PEST SCIENCE 98 . 2 (2025) : 747-757 . |
| APA | Wang, Jin-Yan , Jiang, Jie-Xian , Fan, Neng-Neng , Wu, Hua-Wu , Ji, Xiang-Yun , Wan, Nian-Feng et al. Allicin impacts the susceptibility of Spodoptera exigua to nucleopolyhedrovirus . | JOURNAL OF PEST SCIENCE , 2025 , 98 (2) , 747-757 . |
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BACKGROUNDParasitoid wasps rely on precise immune manipulation to establish infections in their hosts. Tetrastichus brontispae must overcome the robust melanization response of its palm pest host Octodonta nipae, yet the molecular mechanisms regulating to this process remain poorly characterized, particularly regarding microRNA (miRNA)-mediated control of the phenoloxidase (PO) cascade.RESULTSBioinformatic analysis of the O. nipae genome identified 11 melanization-associated miRNAs targeting key immune genes. Dual luciferase detection system assay and RNA binding protein immunoprecipitation results revealed that On-miR-182-5p and On-miR-281-3p regulate the melanization pathway through distinct mechanisms: On-miR-182-5p binds the 5 ' untranslated region (UTR) of OnSPN1, while On-miR-281-3p targets the 3 ' CDS of OnPPO1. Antagomir-mediated knockdown of miR-281-3p increased OnPPO1 messenger RNA (mRNA) levels by 52% and elevated PO activity by 68%, whereas miR-182-5p agomir treatment unexpectedly up-regulated OnSPN1 expression 2.4-fold. Disrupting this regulatory network significantly impaired T. brontispae fitness, reducing emergence rates by 27.3%, skewing offspring sex ratios to 3.8 females per male, and prolonging development time by 48 h.CONCLUSIONOur study reveals a sophisticated immune manipulation strategy where T. brontispae hijacks host miR-182-5p (activator) and miR-281-3p (suppressor) to bidirectionally regulate the PO cascade, simultaneously inhibiting melanization while maintaining microbial defense capacity. These findings provide novel targets for improving biological control strategies against invasive palm pests through miRNA-based interventions. (c) 2025 Society of Chemical Industry.
Keyword ：
melanization melanization microRNA microRNA Octodonta nipae Octodonta nipae phenoloxidase cascade phenoloxidase cascade Tetrastichus brontispae Tetrastichus brontispae
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| GB/T 7714 | Zhang, Huajian , Liao, Guiming , Yin, Jiawei et al. Tetrastichus brontispae exploits host-derived On-miR-281-3p to suppress the phenoloxidase pathway and facilitate its development [J]. | PEST MANAGEMENT SCIENCE , 2025 , 81 (12) : 8151-8162 . |
| MLA | Zhang, Huajian et al. "Tetrastichus brontispae exploits host-derived On-miR-281-3p to suppress the phenoloxidase pathway and facilitate its development" . | PEST MANAGEMENT SCIENCE 81 . 12 (2025) : 8151-8162 . |
| APA | Zhang, Huajian , Liao, Guiming , Yin, Jiawei , Xu, Jiawei , Hou, Youming , Tang, Baozhen . Tetrastichus brontispae exploits host-derived On-miR-281-3p to suppress the phenoloxidase pathway and facilitate its development . | PEST MANAGEMENT SCIENCE , 2025 , 81 (12) , 8151-8162 . |
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本发明提供了一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法。采用界面聚合法制备茚虫威聚脲微胶囊。使用聚脲微胶囊茚虫威毒饵进行红火蚁防治，通过聚脲微胶囊茚虫威毒饵的使用，使茚虫威通过缓慢释放，有效提升毒饵在红火蚁巢穴内的传播范围和覆盖深度，同时延长毒饵的持效期，提高对红火蚁的持续防控效果。
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| GB/T 7714 | 汤宝珍 , 韩延伟 , 张大侠 et al. 一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法 : CN202510489519.9[P]. | 2025-04-18 . |
| MLA | 汤宝珍 et al. "一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法" : CN202510489519.9. | 2025-04-18 . |
| APA | 汤宝珍 , 韩延伟 , 张大侠 , 侯有明 , 廖贵明 , 张婧娴 et al. 一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法 : CN202510489519.9. | 2025-04-18 . |
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