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学者姓名：杨广
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Abstract ：
The development of site-specific genome-editing tools like CRISPR (clustered regularly interspaced short palindromic repeat) and its associated protein, Cas9, is revolutionizing genetic engineering with its highly efficient mechanism, offering the potential for effective pest management. Recently, CRISPR/Cas9 gene-editing has been extensively utilized in the management of the diamondback moth, Plutella xylostella (L.), a highly destructive pest of vegetable crops, for different purposes, such as gene function analysis and genetic control. However, the progress related to this gene-editing tool in P. xylostella has not yet been summarized. This review highlights the progress and applications of CRISPR/Cas9 in uncovering the genes critical for development, reproduction, and insecticide resistance in P. xylostella. Moreover, the progress related to the CRISPR/Cas9 gene drive for population suppression and modifications has also been discussed. In addition to the significant progress made, challenges such as low germline editing efficiency and limited homology-directed repair remain obstacles to its widespread application. To address these limitations, we have discussed the different strategies that are anticipated to improve the efficiency of CRISPR/Cas9, paving the way to it becoming a pivotal tool in sustainable pest management. Therefore, the present review will help researchers in the future enhance the efficiency of the CRISPR/Cas9 system and use it to manage the diamondback moth.
Keyword ：
efficiency of CRISPR/Cas9 system efficiency of CRISPR/Cas9 system gene drive gene drive genetic control genetic control genetic modification genetic modification Plutella xylostella Plutella xylostella site-directed mutagenesis site-directed mutagenesis
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| GB/T 7714 | Asad, Muhammad , Chang, Yanpeng , Liao, Jianying et al. CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (4) . |
| MLA | Asad, Muhammad et al. "CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 4 (2025) . |
| APA | Asad, Muhammad , Chang, Yanpeng , Liao, Jianying , Yang, Guang . CRISPR/Cas9 Genome Editing in the Diamondback Moth: Current Progress, Challenges, and Prospects . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (4) . |
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The diamondback moth (Plutella xylostella), a major lepidopteran pest with a wide host range, presents persistent challenges to sustainable agriculture due to its high adaptability to cruciferous host plants. Although glyoxylate/hydroxypyruvate reductases (GRHPRs) have been well-characterized in plants and humans, their functional role in insects, particularly in host plant adaptation, remains largely unexplored. In this study, we characterized PxGRHPR2, a member of the GRHPR gene family, using a bioinformatics analysis, expression profiling, and CRISPR/Cas9-mediated gene knockout. RT-qPCR analysis showed that PxGRHPR2 was predominantly expressed in larval stage, with the highest transcript levels observed in the second instar and larval midgut tissues. Three homozygous PxGRHPR2 knockout strains were successfully generated using CRISPR/Cas9 system. Mutation of PxGRHPR2 led to significant reductions in larval weight, survival, and eclosion rates when larvae were fed on radish seedlings, whereas no such effects were observed under artificial diet conditions. These findings suggest that PxGRHPR2 plays a critical role in detoxification and metabolic regulation, thereby facilitating host plant adaptability in P. xylostella. Overall, this study provides new insights into insect-plant interactions and identifies PxGRHPR2 as a potential molecular target for developing sustainable pest management strategies.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 detoxification detoxification host plant adaptation host plant adaptation Plutella xylostella Plutella xylostella PxGRHPR2 PxGRHPR2
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| GB/T 7714 | Zaheer, Uroosa , Munir, Faisal , Qiao, Qingxuan et al. Functional Role of the PxGRHPR2 Gene in the Host Plant Adaptation of Diamondback Moth (Plutella xylostella) [J]. | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 120 (1) . |
| MLA | Zaheer, Uroosa et al. "Functional Role of the PxGRHPR2 Gene in the Host Plant Adaptation of Diamondback Moth (Plutella xylostella)" . | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY 120 . 1 (2025) . |
| APA | Zaheer, Uroosa , Munir, Faisal , Qiao, Qingxuan , Salum, Yussuf Mohamed , Abbas, Anam Noreen , Tariq, Mubashir et al. Functional Role of the PxGRHPR2 Gene in the Host Plant Adaptation of Diamondback Moth (Plutella xylostella) . | ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 120 (1) . |
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The /3-tubulin gene is essential for reproductive development, especially for male fertility, in different insects including Bombyx mori and Drosophila melanogaster. Targeting reproductive genes such as /3-tubulin offers a promising approach to pest control that is more sustainable than chemical pesticides. However, there is limited research on the functional role of /3-tubulin in Plutella xylostella, a highly damaging pest of vegetable crops. In the present study, we first identified and cloned the /3-tubulin gene in P. xylostella (Px/3tubulin-1). Px beta tubulin-1 protein contains two conserved domains of Tubulin and Tubulin-C, and /3-tubulin were conserved in the Lepidoptera. Spatiotemporal expression analysis revealed that Px/3tubulin-1 was highly expressed in male pupae, adult males, and testes, suggesting its testis-specific function. Using CRISPR/Cas9 technology, we generated two homozygous Px/3tubulin-1 mutant strains of P. xylostella. Mutant strains exhibited significantly lower egg production and hatching rates compared with the wild type. Dissection and measurement of reproductive organs revealed that the testes and bursa copulatrix in mutant strains were significantly reduced in size compared with the wild type. In conclusion, Px/3tubulin-1 is vital for male fertility as it influences the development of reproductive organs and can be a potential target for the control of P. xylostella.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 Diamondback moth Diamondback moth Px/3tubulin-1 Px/3tubulin-1 Reproductive fitness Reproductive fitness
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| GB/T 7714 | Liao, Jianying , Chen, Jing , Liu, Dan et al. Molecular and functional characterization of a /3-tubulin gene in Plutella xylostella [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 300 . |
| MLA | Liao, Jianying et al. "Molecular and functional characterization of a /3-tubulin gene in Plutella xylostella" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 300 (2025) . |
| APA | Liao, Jianying , Chen, Jing , Liu, Dan , Li, Jianwen , Chen, Jinzhi , Sun, Cuiying et al. Molecular and functional characterization of a /3-tubulin gene in Plutella xylostella . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 300 . |
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【目的】小菜蛾Plutella xylostella是世界性的十字花科蔬菜害虫。本研究旨在明确视蛋白基因LW-opsin突变对小菜蛾生长发育和繁殖能力的影响，为开发小菜蛾遗传防控新靶标提供理论依据。【方法】实验室条件下，利用年龄-龄期两性生命表测定小菜蛾野生型品系G88和LW-opsin突变品系LW-13的发育历期、存活率、繁殖力、生命期望值和生命表参数。【结果】LW-opsin突变延长了小菜蛾成虫前历期[G88:（12.45±0.06） d; LW-13:（13.45±0.25） d]和总产卵前期[G88:（12.59±0.08） d; LW-13:（13.57±0.30） d],缩短了成虫寿命[G88:（6.84±0.02） d; LW-13:（5.73±0.07） d]和产卵期[G88:（6.09±0.10） d; LW-13:（5.14±0.34） d]。LW-opsin突变降低了小菜蛾幼虫、蛹和成虫的存活率。G88品系种群特定年龄存活率从18日龄起开始急剧下降，LW-13品系种群特定年龄存活率则在4日龄时已降至25%。LW-opsin突变降低了种群特定年龄繁殖值、特定年龄-龄期生命期望值、内禀增长率[G88:（0.31±0.01） d
Keyword ：
小菜蛾 小菜蛾 生命表 生命表 生长发育 生长发育 突变品系 突变品系 繁殖 繁殖 视蛋白 视蛋白
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| GB/T 7714 | 陈韶萍 , 邱荣洲 , 池美香 et al. 视蛋白基因LW-opsin突变影响小菜蛾的生长发育和繁殖能力 [J]. | 昆虫学报 , 2025 , 68 (01) : 82-88 . |
| MLA | 陈韶萍 et al. "视蛋白基因LW-opsin突变影响小菜蛾的生长发育和繁殖能力" . | 昆虫学报 68 . 01 (2025) : 82-88 . |
| APA | 陈韶萍 , 邱荣洲 , 池美香 , 陈燕婷 , 杨广 . 视蛋白基因LW-opsin突变影响小菜蛾的生长发育和繁殖能力 . | 昆虫学报 , 2025 , 68 (01) , 82-88 . |
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Knock-in efficiency via CRISPR/Cas9-based homology-directed repair (HDR) is low in lepidopteran insects due to predominance of knockout through non-homologous end joining (NHEJ) pathway. DNA ligase 4 (LIG4), a core protein of NHEJ complex and double-strand break (DSB) binding repair, is a promising target for enhancing HDR efficiency in diverse species. However, the role of Lig4 in modulating HDR efficiency in Plutella xylostella, a highly destructive pest of vegetable crops, remains unclear. The PxLig4 gene was identified from the P. xylostella genome with 4 conserved domains: ATP-dependent DNA ligase core-adenylation middle domain, ATP-dependent DNA ligase core-adenylation N-terminal domain, ATP-dependent DNA ligase core-adenylation C-terminal domain, and BRCA1 C terminus domain. Multiple sequence alignment and phylogenetic analysis confirmed strong conservation of PxLig4 among Lepidoptera. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis showed that PxLig4 was highly expressed in eggs, female adults, and male adults, particularly in the ovary, followed by the testis, fat body, and midgut tissues. A homozygous PxLig4 knockout line (Delta PxLig4) with a 2-bp deletion was successfully generated by using the CRISPR/Cas9 technique. Delta PxLig4 exhibited significant developmental and reproductive defects. A donor vector to knock in the enhanced green fluorescent protein (EGFP) gene at the eye color gene PxKmo was developed to assess knock-in efficiency. The knock-in efficiency in Delta PxLig4 was 2.86%, significantly higher than the 0.26% observed in the wild-type P. xylostella. These results implicate Lig4 as a supporting factor for NHEJ and demonstrate that suppression of PxLig4 enhances HDR efficiency in P. xylostella, providing a potential strategy to improve the efficiency of gene knock-in in insect pests.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 diamondback moth diamondback moth DNA ligase 4 DNA ligase 4 knock-in efficiency knock-in efficiency
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| GB/T 7714 | Munir, Faisal , Zaheer, Uroosa , Lin, Sheng et al. Knockout of Ligase4 gene enhances homology-directed repair efficiency in Plutella xylostella [J]. | INSECT SCIENCE , 2025 . |
| MLA | Munir, Faisal et al. "Knockout of Ligase4 gene enhances homology-directed repair efficiency in Plutella xylostella" . | INSECT SCIENCE (2025) . |
| APA | Munir, Faisal , Zaheer, Uroosa , Lin, Sheng , Abbas, Anam Noreen , Tariq, Mubashir , Liao, Jianying et al. Knockout of Ligase4 gene enhances homology-directed repair efficiency in Plutella xylostella . | INSECT SCIENCE , 2025 . |
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The invasive pest Frankliniella occidentalis (western flower thrip, WFT) severely impacts agricultural production. RNA interference (RNAi) has emerged as a viable alternative to chemical control methods. Trehalose-6-phosphate synthase (TPS) is an important enzyme for the synthesis of trehalose in insects. The TPS gene is a potential target for RNAi-based pest control; however, its efficacy against WFTs remains unclear. Feeding with the synthesized dsFoTPS to WFT significantly increased the mortalities of first- and second-instar nymphs and adults and significantly decreased the number of eggs laid by adults within 72 h; the expression of FoTPS was significantly down-regulated in 24, 48, and 72 h. Ingestion of WFTs on Escherichia coli expressing dsFoTPS led to a marked increase in the total pre-reproductive period; reductions in survival rate, adult longevity, oviposition days, fecundity, peak reproduction value, the intrinsic rate of increase, the finite rate of increase, and the net reproductive rate; and an extension of the mean generation time. TPS gene expression was significantly down-regulated on days 7 and 28. A population simulation of WFTs fed with E. coli expressing dsFoTPS indicated that the population suppression was reduced to 1/34 of the control in 100 d. Oral delivery of E. coli expressing dsFoTPS effectively inhibited the survival, fecundity, and population growth of WFTs, offering a novel approach and rationale for the prevention and control of WFTs.
Keyword ：
bacterially expressed dsRNA bacterially expressed dsRNA feeding feeding life table life table population suppression population suppression trehalose-6-phosphate synthase trehalose-6-phosphate synthase western flower thrip western flower thrip
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| GB/T 7714 | Lin, Tao , Chen, Xiaoyu , Chen, Ying et al. Effects of Orally Delivered Double-Stranded RNA of Trehalose-6-Phosphate Synthase on the Population of Frankliniella occidentalis [J]. | INSECTS , 2025 , 16 (6) . |
| MLA | Lin, Tao et al. "Effects of Orally Delivered Double-Stranded RNA of Trehalose-6-Phosphate Synthase on the Population of Frankliniella occidentalis" . | INSECTS 16 . 6 (2025) . |
| APA | Lin, Tao , Chen, Xiaoyu , Chen, Ying , Chen, Ting , Liang, Xueyi , Wei, Hui et al. Effects of Orally Delivered Double-Stranded RNA of Trehalose-6-Phosphate Synthase on the Population of Frankliniella occidentalis . | INSECTS , 2025 , 16 (6) . |
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The forkhead box (Fox) family of transcription factors is essential for controlling physiological functions, metabolism and development in insects. However, the role of the Fox gene in the diamondback moth, Plutella xylostella, a major agricultural pest of cruciferous crops, is still unknown. The purpose of this work was to characterize the PxFox1 gene and investigate its role in the reproduction and development of P. xylostella. The Fox gene in P. xylostella (PxFox1) is identified from the P. xylostella genome. The Fox gene was conserved in lepidoptera with the presence of Forkhead domain. PxFox1 was expressed in different developmental stages and tissues of P. xylostella, highly in the female adult and integument. The PxFox1 knockout mutants were generated by using the CRISPR/Cas9 technique. The significant reduction in the number of eggs and hatching rate was observed in mutants as compared with the wild-type. Meanwhile, an extended larval duration, shortened pupal phase, and reduced adult lifespan were observed in the mutants. Furthermore, PxFox1 mutants displayed significant phenotypic abnormalities such as malformed wings, irregular body segmentation, and reduced body size, highlighting the gene's essential role in the morphological development. These results showed that PxFox1 is necessary for P. xylostella development, survival, and reproduction. Targeting PxFox1 could provide a potential genetic pest control strategy for managing P. xylostella populations, offering new insights into insect gene regulation for sustainable pest management.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 Forkhead transcription factor Forkhead transcription factor Gene knockout Gene knockout Pest management Pest management Plutella xylostella Plutella xylostella
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| GB/T 7714 | Abbas, Anam Noreen , Tariq, Mubashir , Munir, Faisal et al. Roles of Fox gene in the development and reproduction of diamondback moth [J]. | JOURNAL OF INSECT PHYSIOLOGY , 2025 , 165 . |
| MLA | Abbas, Anam Noreen et al. "Roles of Fox gene in the development and reproduction of diamondback moth" . | JOURNAL OF INSECT PHYSIOLOGY 165 (2025) . |
| APA | Abbas, Anam Noreen , Tariq, Mubashir , Munir, Faisal , Zaheer, Uroosa , Adam, Ibrahim , Asad, Muhammad et al. Roles of Fox gene in the development and reproduction of diamondback moth . | JOURNAL OF INSECT PHYSIOLOGY , 2025 , 165 . |
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[目的]茶小绿叶蝉Empoasca onukii体表覆盖的纳米网粒体颗粒具有防蜜露粘黏、抗杀虫剂渗透等屏障作用。本研究尝试探索造成叶蝉体表网粒体防护层破坏的方法,跟踪分析茶小绿叶蝉成虫网粒体防护层破坏后的分泌节律及修复行为,并解析网粒体涂抹器的微观形态,以期阐明茶小绿叶蝉涂抹修复纳米网粒体防护层的具体特征。[方法]用清水喷雾、喷粉、高湿处理以及用添加表面活性剂的杀虫剂喷雾处理茶小绿叶蝉成虫,用指示剂药斑法及扫描电子显微镜(scanning electron microscope,SEM)观察这些处理对成虫翅面网粒体的破坏效果;以有机硅助剂分别喷雾以及单翅涂抹成虫两种方式进行破坏处理,并以不做任何处理的成虫为对照组,通过可拍照显微镜记录成虫翅面出现网粒体白斑次数,统计不同处理组成虫网粒体分泌节律变化;通过Vegas软件分析叶蝉网粒体涂抹动作,利用器官破坏法确定网粒体涂抹器所在部位,并通过SEM观察其微观结构。[结果]清水喷雾、粉尘和高湿环境不会对茶小绿叶蝉成虫体表网粒体防护层造成破坏,喷雾有机硅助剂可在成虫翅面产生明显药斑,SEM图片显示网粒体与残留有机硅助剂团聚结块并掉落;有机硅助剂喷雾处理组成虫网粒体的分泌间隔时长为(22.3±1.8) h,比对照组缩短了约8.0 h,5 d内处理组成虫网粒体分泌频次为(8±1)次,比对照组高出约3次;成虫往体表涂抹修饰网粒体时前后足的振动频率显著高于清扫异物的抖动频率;器官破坏法处理后足胫节微毛列造成叶蝉无法正常涂抹网粒体,这可能是由涂抹器微毛顶端反向弯曲造成的。[结论]有机硅助剂可破坏茶小绿叶蝉成虫体表网粒体防护层,有机硅助剂处理后叶蝉通过缩短网粒体的分泌间隔时长来修复网粒体防护层;叶蝉前后足振动频率以及胫节微毛末端的正常弯钩是叶蝉完成纳米网粒体颗粒涂抹的关键。
Keyword ：
体表 体表 有机硅助剂 有机硅助剂 网粒体 网粒体 网粒体分泌 网粒体分泌 网粒体涂抹器 网粒体涂抹器 茶小绿叶蝉 茶小绿叶蝉
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| GB/T 7714 | 屈政 , 杨广 , 杨小红 et al. 茶小绿叶蝉体表纳米网粒体防护层的破坏与修复 [J]. | 昆虫学报 , 2024 , (06) : 1-12 . |
| MLA | 屈政 et al. "茶小绿叶蝉体表纳米网粒体防护层的破坏与修复" . | 昆虫学报 06 (2024) : 1-12 . |
| APA | 屈政 , 杨广 , 杨小红 , 林美珍 . 茶小绿叶蝉体表纳米网粒体防护层的破坏与修复 . | 昆虫学报 , 2024 , (06) , 1-12 . |
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[目的]评估芳樟醇、茉莉酸甲酯和香叶醇3种植物挥发性化合物对黄胸蓟马Thrips hawaiiensis和南方小花蝽Orius strigicollis的吸引作用,为利用植物挥发性化合物田间诱集南方小花蝽防控黄胸蓟马提供依据。[方法]采用Y形嗅觉仪测定10,0.1和0.001 g/L以及980 g/L芳樟醇、茉莉酸甲酯和香叶醇对黄胸蓟马成虫和南方小花蝽5龄若虫和成虫的吸引率,测定0.1,10和980 g/L香叶醇对南方小花蝽和黄胸蓟马成虫吸引作用的时间效应以及吸引率;并进一步在田间大棚条件测定10 g/L香叶醇对黄胸蓟马和南方小花蝽的吸引率。[结果]与对照组(石蜡油)比,茉莉酸甲酯对黄胸蓟马成虫和南方小花蝽5龄若虫的吸引率差异不显著,980 g/L芳樟醇对黄胸蓟马成虫吸引率显著提高,但对南方小花蝽5龄若虫和成虫的吸引率差异不显著;980 g/L香叶醇对黄胸蓟马成虫的吸引率显著提高,高浓度(10和0.1 g/L)香叶醇对南方小花蝽成虫的吸引率均显著提高,但对南方小花蝽若虫的吸引率差异不显著。不同浓度香叶醇对黄胸蓟马和南方小花蝽成虫的吸率具有显著的时间效应,且980和10 g/L香叶醇在处理后2 h时对南方小花蝽成虫的吸引率显著高于其他浓度的,并且无论是否被黄胸蓟马成虫为害,980和10 g/L香叶醇处理的青椒苗对南方小花蝽成虫的吸引率显著高于清水对照。与对照组(清水)比,田间大棚喷洒10 g/L香叶醇的处理组在5 h内诱集的黄胸蓟马成虫数量差异不显著,但诱集的南方小花蝽成虫数量显著提高。[结论]芳樟醇、茉莉酸甲酯和香叶醇3种植物挥发性化合物对黄胸蓟马和南方小花蝽的吸引率存在差异,10 g/L香叶醇能够在5 h内有效诱集南方小花蝽成虫,但不显著吸引黄胸蓟马,这些结果为利用香叶醇诱集南方小花蝽防控黄胸蓟马提供了理论依据和技术支撑。
Keyword ：
南方小花蝽 南方小花蝽 吸引作用 吸引作用 植物挥发物 植物挥发物 诱集 诱集 香叶醇 香叶醇 黄胸蓟马 黄胸蓟马
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| GB/T 7714 | 林涛 , 郭雅清 , 陈晓瑜 et al. 三种植物挥发性化合物对黄胸蓟马和南方小花蝽的吸引作用及诱集效率 [J]. | 昆虫学报 , 2024 , (08) : 2-7,9-11 . |
| MLA | 林涛 et al. "三种植物挥发性化合物对黄胸蓟马和南方小花蝽的吸引作用及诱集效率" . | 昆虫学报 08 (2024) : 2-7,9-11 . |
| APA | 林涛 , 郭雅清 , 陈晓瑜 , 杨风花 , 杨广 , 魏辉 . 三种植物挥发性化合物对黄胸蓟马和南方小花蝽的吸引作用及诱集效率 . | 昆虫学报 , 2024 , (08) , 2-7,9-11 . |
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Earth's rotation shapes a 24-h cycle, governing circadian rhythms in organisms. In mammals, the core clock genes, CLOCK and BMAL1, are regulated by PERIODs (PERs) and CRYPTOCHROMEs (CRYs), but their roles remain unclear in the diamondback moth, Plutella xylostella. To explore this, we studied P. xylostella, which possesses a simplified circadian system compared to mammals. In P. xylostella, we observed rhythmic expressions of the Pxper and Pxcry2 genes in their heads, with differing phases. In vitro experiments revealed that PxCRY2 repressed monarch butterfly CLK:BMAL1 transcriptional activation, while PxPER and other CRY-like proteins did not. However, PxPER showed an inhibitory effect on PxCLK/PxCYCLE. Using CRISPR/Cas9, we individually and in combination knocked out Pxper and Pxcry2, then conducted gene function studies and circadian transcriptome sequencing. Loss of either Pxper or Pxcry2 eliminated the activity peak after lights-off in light-dark cycles, and Pxcry2 loss reduced overall activity. Pxcry2 was crucial for maintaining endogenous rhythms in constant darkness. Under light-dark conditions, 1 098 genes exhibited rhythmic expression in wild-type P. xylostella heads, with 749 relying on Pxper and Pxcry2 for their rhythms. Most core clock genes lost their rhythmicity in Pxper and Pxcry2 mutants, while Pxcry2 sustained rhythmic expression, albeit with reduced amplitude and altered phase. Additionally, rhythmic genes were linked to biological processes like the spliceosome and Toll signaling pathway, with these rhythms depending on Pxper or Pxcry2 function. In summary, our study unveils differences in circadian rhythm regulation by Pxper and Pxcry2 in P. xylostella. This provides a valuable model for understanding circadian clock regulation in nocturnal animals. The earth's rotation drives a roughly 24-h cycle, governing circadian rhythms in organisms. In mammals, master genes CLOCK:BMAL1 are repressed by multiple PERIODs (PERs) and CRYPTOCHROMEs (CRYs). Differences in the regulation of behavior by and oscillations in PERs and CRYs are not known. Plutella xylostella, the diamondhead moth, possessing a simplified PER/CRY system, was investigated. Here, we applied the CRISPR/Cas9 technology to individually and in combination knock out the circadian clock genes of Pxper and Pxcry2. Subsequently, we conducted in vivo gene function studies, coupled with time-series transcriptome sequencing to analyze the functional differences of Pxper and Pxcry2. # image
Keyword ：
CRY2 CRY2 daily transcriptome daily transcriptome locomotor behavior locomotor behavior PERIOD PERIOD Plutella xylostella Plutella xylostella
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| GB/T 7714 | Chen, Wenfeng , Wang, Danfeng , Yu, Lingqi et al. Comparative analysis of locomotor behavior and head diurnal transcriptome regulation by PERIOD and CRY2 in the diamondback moth [J]. | INSECT SCIENCE , 2024 , 31 (6) : 1697-1720 . |
| MLA | Chen, Wenfeng et al. "Comparative analysis of locomotor behavior and head diurnal transcriptome regulation by PERIOD and CRY2 in the diamondback moth" . | INSECT SCIENCE 31 . 6 (2024) : 1697-1720 . |
| APA | Chen, Wenfeng , Wang, Danfeng , Yu, Lingqi , Zhong, Wenmiao , Yuan, Yao , Yang, Guang . Comparative analysis of locomotor behavior and head diurnal transcriptome regulation by PERIOD and CRY2 in the diamondback moth . | INSECT SCIENCE , 2024 , 31 (6) , 1697-1720 . |
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