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学者姓名：魏太云
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Abstract ：
Xenophagy is an important antibacterial defense mechanism that many organisms use to engulf intracellular pathogens. However, the mechanisms of xenophagy triggered by insect-borne plant bacteria are not well understood. Candidatus Liberibacter asiaticus (CLas) causes Huanglongbing, which poses a serious threat to citrus production. CLas is a phloem-limited unculturable bacterium that is transmitted by the Asian citrus psyllid in a persistent and propagative manner in nature. Here, we found that CLas infection in the gut of psyllids triggered a mild and anti-bacterial xenophagy. Xenophagy limited excessive propagation of CLas to maintain psyllid survival, because overload of CLas was detrimental to psyllid life. Furthermore, the outer membrane beta-barrel protein (OMBB) of CLas is the key secreted protein that induces xenophagy in psyllids by interacting with ATG8 and ATG14. OMBB can independently induce autophagy in psyllid and non-host cells. Together, these results revealed that an insect-borne plant bacterium activates mild xenophagy to control its propagation, thereby achieving persistent infection in insect vectors.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus Huanglongbing Huanglongbing Persistent infection Persistent infection Psyllid Psyllid Xenophagy Xenophagy
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| GB/T 7714 | Yu, Zhongkai , Guo, Yuxin , Chen, Hongyan et al. A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 297 . |
| MLA | Yu, Zhongkai et al. "A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection" . | MICROBIOLOGICAL RESEARCH 297 (2025) . |
| APA | Yu, Zhongkai , Guo, Yuxin , Chen, Hongyan , Wan, Wenqiang , Hu, Mengting , Li, You et al. A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection . | MICROBIOLOGICAL RESEARCH , 2025 , 297 . |
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High-throughput sequencing was used to identify and characterize a novel marafivirus from the weed Leptochloa chinensis, which was tentatively named "Leptochloa chinensis marafivirus" (LcMV). The complete genome of the virus consists of 6,178 base pairs, and its nucleotide sequence is 73.82% identical to that of Sorghum almum marafivirus, which is a member of the genus Marafivirus within the family Tymoviridae. The LcMV genome contains a relatively large open reading frame (ORF) encoding a single polyprotein (220.6 kDa) with five functional domains (methyltransferase, papain-like protease, helicase, RNA-dependent RNA polymerase, and coat proteins), which is a characteristic of members of this genus. Furthermore, a 16-nucleotide conserved marafibox sequence was identified at nucleotide positions 5341-5356. The coat protein of LcMV is 68.02% identical to that of Sorghum almum marafivirus. Phylogenetic analysis based on nucleotide and polyprotein sequences showed that LcMV is closely related to members of the genus Marafivirus. Our findings support the classification of LcMV as a member of a new species within this genus. This is the first report of a marafivirus infecting Leptochloa chinensis, a very important weed of rice.
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| GB/T 7714 | Muqmirah, Guangming , Xiao, Guangming , Jie, Pengpeng et al. Sequence analysis and genome organization of a new marafivirus from Leptochloa chinensis [J]. | ARCHIVES OF VIROLOGY , 2025 , 170 (2) . |
| MLA | Muqmirah, Guangming et al. "Sequence analysis and genome organization of a new marafivirus from Leptochloa chinensis" . | ARCHIVES OF VIROLOGY 170 . 2 (2025) . |
| APA | Muqmirah, Guangming , Xiao, Guangming , Jie, Pengpeng , Xu, Zhongtian , Wei, Taiyun , Wang, Hui . Sequence analysis and genome organization of a new marafivirus from Leptochloa chinensis . | ARCHIVES OF VIROLOGY , 2025 , 170 (2) . |
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Insect melanization triggered by the conversion of prophenoloxidase to active phenoloxidase via serine proteases (SPs) is an important immediate immune response. However, how phytoplasmas evade this immune response to promote their propagation in insect vectors remains unknown. Here, we demonstrate that infection of leafhopper vectors with rice orange leaf phytoplasma (ROLP) activates the mild melanization response in hemolymph. ROLP-encoded effector protein SRP1 is highly expressed in leafhopper hemolymph, where it competitively binds to SP2, thereby inhibiting SP2-mediated cleavage of prophenoloxidase into active phenoloxidase. Consequently, microinjection of SRP1 effectively suppresses the melanization response and enhances ROLP propagation. The histidine residue at position 23 of SRP1 is essential for SRP1-SP2 interaction, and the mutation of this position abolishes its ability to inhibit such SP2-meidated cleavage, ultimately promoting melanization response and inhibiting ROLP propagation. Our findings provide insights into how phytoplasmas antagonize insect melanization response to facilitate their persistent transmission.
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| GB/T 7714 | Zhang, Xiao-Feng , Li, Zhanpeng , Qiu, Jiaxin et al. A phytoplasma effector suppresses insect melanization immune response to promote pathogen persistent transmission [J]. | SCIENCE ADVANCES , 2025 , 11 (5) . |
| MLA | Zhang, Xiao-Feng et al. "A phytoplasma effector suppresses insect melanization immune response to promote pathogen persistent transmission" . | SCIENCE ADVANCES 11 . 5 (2025) . |
| APA | Zhang, Xiao-Feng , Li, Zhanpeng , Qiu, Jiaxin , Zhang, Ruonan , Jiang, Zhoumian , Wang, Tengfei et al. A phytoplasma effector suppresses insect melanization immune response to promote pathogen persistent transmission . | SCIENCE ADVANCES , 2025 , 11 (5) . |
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In the field, 80% of plant viruses are transmitted by insect vectors. When ingested by a sap-sucking insect such as Recilia dorsalis, persistently transmitted viruses such as rice stripe mosaic virus (RSMV) infect the gut epithelium and eventually pass to the salivary glands where they will be transmitted to the next rice (Oryza sativa) plant. To efficiently exploit insect vectors for transmission, plant viruses must overcome various immune mechanisms within the vectors, including autophagy. However, understanding how plant viruses overcome insect autophagic defenses remains limited. In this study, we provide evidence that infection with RSMV triggers an autophagic antiviral response in leafhopper cells. In this response, the G protein of RSMV binds to a leafhopper AMP-activated protein kinase (AMPK), leading to enhanced phosphorylation of Beclin-1 (BECN1), thereby inducing autophagy. Knockdown of AMPK and genes encoding members of the phosphoinositide 3-kinase (PI3K) complex composed of the autophagy-related protein 14 (ATG14), BECN1, and vacuolar protein sorting 34 (VPS34) facilitated viral infection in leafhoppers. To suppress leafhopper-induced autophagy, RSMV M protein specifically interacts with ATG14, resulting in the disintegration of PI3K complexes. This leads to reduced phosphatidylinositol-3-phosphate content and thus inhibits the G-protein- induced autophagy. Our study sheds light on the mechanism by which this rice virus evades insect autophagy antiviral defenses.
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| GB/T 7714 | Zhang, Ruonan , Wang, Tengfei , Cheng, Yu et al. Rice stripe mosaic virus M protein antagonizes G-protein-induced antiviral autophagy in insect vectors [J]. | PLOS PATHOGENS , 2025 , 21 (4) . |
| MLA | Zhang, Ruonan et al. "Rice stripe mosaic virus M protein antagonizes G-protein-induced antiviral autophagy in insect vectors" . | PLOS PATHOGENS 21 . 4 (2025) . |
| APA | Zhang, Ruonan , Wang, Tengfei , Cheng, Yu , Qiu, Jiaxin , Jia, Dongsheng , Chen, Hongyan et al. Rice stripe mosaic virus M protein antagonizes G-protein-induced antiviral autophagy in insect vectors . | PLOS PATHOGENS , 2025 , 21 (4) . |
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The citrus disease Huanglongbing (HLB) in Asia and the US is caused by Candidatus Liberibacter asiaticus (CLas), which is primarily transmitted by Diaphorina citri, also known as Asian citrus psyllid in a persistent and propagative manner. However, the exact mechanisms underlying CLas circulation within D. citri remain largely unclear. Here, immunofluorescence microscopy and electron microscopy were utilized to track the sequential infection of CLas in D. citri, from alimentary canal to salivary glands, and ultimately to the plant host. CLas was found to initially infect the epithelium of filter chamber, after which it rapidly spreads to visceral muscles for further infection throughout the alimentary canal. The rapid spread in D. citri adults causes the duration of CLas circulation to be as short as 9 days. The duration of latent period may be explained by the recruitment of cytoskeletal alpha-actinin by the outer membrane protein (OMP) of CLas. Inhibition of actin filament or knocking down the expression of alpha-actinin significantly suppresses CLas cytoskeleton-dependent infection in and spread among D. citri organs. Injection of prokaryotically expressed OMP into D. citri also recruits alpha-actinin, resembling the natural infection of CLas. Our studies showed that CLas exploits alpha-actinin and remolds actin machinery of D. citri for overcoming the midgut release barrier, facilitating its circulation in the vector. By shedding light on these mechanisms, this report reveals more detailed mechanisms in CLas infection in D. citri, and offers a plausible explanation for rapid dissemination of HLB in nature from the perspective of psyllid transmission.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus Circulative infection Circulative infection Cytoskeleton Cytoskeleton Diaphorina citri Diaphorina citri
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| GB/T 7714 | Li, Zhiqiang , Yang, Xiao , Guo, Yuxin et al. Candidatus Liberibacter asiaticus exploits cytoskeletal system of psyllid vector for circulative propagative infection [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 292 . |
| MLA | Li, Zhiqiang et al. "Candidatus Liberibacter asiaticus exploits cytoskeletal system of psyllid vector for circulative propagative infection" . | MICROBIOLOGICAL RESEARCH 292 (2025) . |
| APA | Li, Zhiqiang , Yang, Xiao , Guo, Yuxin , Zhang, Xiaofeng , Li, You , Kuo, Yen-Wen et al. Candidatus Liberibacter asiaticus exploits cytoskeletal system of psyllid vector for circulative propagative infection . | MICROBIOLOGICAL RESEARCH , 2025 , 292 . |
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Phloem-inhabiting unculturable bacterial pathogens are persistently transmitted by insect vectors. However, how they evade insect immune responses to ensure persistent transmission remains unknown. The important melanization immune response in insects is triggered by cleavage of prophenoloxidase (PPO) into active phenoloxidase (PO) via clip-domain serine proteases (CLIPs). Here, we demonstrate that infection of Candidatus Liberibacter asiaticus (CLas) in psyllid vectors activates the peptidoglycan recognition protein (PGRP)-CLIP1-CLIP4-PPO-PO signaling cascade to induce a mild melanization response, ensuring persistent CLas infection without causing significant insect fitness costs to the insect. A CLas-encoded secretory protein, SDE3230, directly interacts with PGRP and suppresses its activity in transducing this signaling cascade. CLIP4 cleaves PPO between arginine 125 and methionine 126 residues to active PO to induce melanization, and this cleavage pattern in psyllid is distinct from other insects. However, SDE3230 competitively binds to this cleavage site of PPO with CLIP4, thereby suppressing PPO effective cleavage. Collectively, these findings reveal the dual role of SDE3230 in facilitating the mild melanization response, benefitting persistent CLas infection and insect fitness.IMPORTANCEPsyllid-borne huanglongbing is the most destructive citrus disease worldwide, causing billions of dollars in annual production losses and threatening the entire citrus industry. Currently, the mechanism by which the causal agent Candidatus Liberibacter asiaticus (CLas) antagonizes psyllid innate immune responses to facilitate its coexistence with psyllid vectors is still unknown. Here, we report that CLas exploits the highly expressed secretory protein SDE3230 in psyllids to suppress the important melanization immune response in hemolymph via inhibiting the pattern recognition receptor PGRP activity and the cleavage of prophenoloxidase into active phenoloxidase by clip-domain serine proteases. The pattern of PPO cleavage is novel, and this process ultimately ensures persistent CLas infection and insect fitness. Our findings provide insights into how CLas has evolved novel strategies to evade the insect melanization response, thereby facilitating persistent CLas transmission.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus huanglongbing huanglongbing melanization melanization persistent infection persistent infection psyllid psyllid secretory protein secretory protein
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| GB/T 7714 | Li, You , Du, Yu , Ren, Dongsheng et al. Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection [J]. | MBIO , 2025 , 16 (10) . |
| MLA | Li, You et al. "Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection" . | MBIO 16 . 10 (2025) . |
| APA | Li, You , Du, Yu , Ren, Dongsheng , Bin, Yu , Chen, Qian , Wei, Taiyun . Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection . | MBIO , 2025 , 16 (10) . |
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Huanglongbing (HLB) is a destructive disease that threatens the citrus industry worldwide. It is associated with "Candidatus Liberibacter asiaticus" (CLas) and vectored by Diaphorina citri, known as the Asian citrus psyllid (ACP). Australian finger lime (Citrus australasica), a promising candidate for HLB tolerance, was examined in response to CLas-infected ACPs through comprehensive transcriptomic profiling to identify key antimicrobial peptides (AMPs) and immune-related pathways. GO, KEGG, and genome enrichment analysis found that most DEGs were enriched in metabolic pathways, biosynthesis of secondary metabolites, and hormone signal transduction. Furthermore, immune pathways associated with pathogen recognition and defense mechanisms were characterized. DEGs were screened and confirmed through quantitative real-time qPCR (RT-qPCR). By analyzing gene expression in C. australasica, we identified several potential AMPs with significant antimicrobial properties. CaAMP1, CaAMP2, CaAMP4, and CaShepharin exhibited strong inhibitory effects against multiple bacterial suspensions with minimum EC50 values. Similarly, CaAMP4 also caused a significant reduction in CLas titer through Agrobacterium-mediated transient expression. This study provides a novel perspective for understanding the transcriptomic information on C. australasica in response to CLas-infected ACPs. These findings offer valuable insights into the development of AMP-based, environmentally sustainable strategies for managing HLB and enhancing citrus plant resilience.
Keyword ：
Asian citrus psyllid(Diaphorina citri) Asian citrus psyllid(Diaphorina citri) Australian finger lime Australian finger lime defense mechanisms defense mechanisms gene expression gene expression HLB HLB immune pathways immune pathways transcriptomicinformation transcriptomicinformation
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| GB/T 7714 | Younas, Muhammad , Wang, Chuang , Hassan, Mohamed Fathi et al. Comprehensive Transcriptomic Profiling of Citrus australasica Unveils Antimicrobial Peptides and Immune Pathways for Huanglongbing Tolerance [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (27) : 16847-16859 . |
| MLA | Younas, Muhammad et al. "Comprehensive Transcriptomic Profiling of Citrus australasica Unveils Antimicrobial Peptides and Immune Pathways for Huanglongbing Tolerance" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 27 (2025) : 16847-16859 . |
| APA | Younas, Muhammad , Wang, Chuang , Hassan, Mohamed Fathi , Li, Wenqing , Zheng, Zheng , Bin, Yu et al. Comprehensive Transcriptomic Profiling of Citrus australasica Unveils Antimicrobial Peptides and Immune Pathways for Huanglongbing Tolerance . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (27) , 16847-16859 . |
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在农业生态系统中，植物病毒病流行的生物因素包括病毒、介体、寄主植物。介体昆虫、病毒、寄主植物在漫长的进化中形成复杂的互作关系。明确昆虫-病毒-植物互作的分子机制，有助于制定精准、高效的虫传病毒病控制策略。在昆虫的天然免疫和植物的防御反应过程中，自噬可降解病毒粒体，或清除被病毒侵染的细胞器，抑制病毒的侵染。为对抗来自寄主或介体昆虫的免疫攻击，有些病毒进化出操纵自噬的反防御策略，从而促进自身的侵染。本文围绕昆虫介体/植物寄主与病毒的关系，阐述昆虫/植物的细胞自噬调控病毒侵染的机制，论述自噬参与的免疫稳态在病毒侵染昆虫介体过程中的作用，为昆虫、病毒、植物三者关系提供重要的理论基础，昆虫/植物的自噬途径也可能成为控制病害传播和侵染策略的潜在靶标。
Keyword ：
昆虫 昆虫 植物 植物 病毒 病毒 细胞凋亡 细胞凋亡 细胞自噬 细胞自噬
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| GB/T 7714 | 万文强 , 魏太云 , 陈倩 . 昆虫/植物的细胞自噬调控病毒侵染的研究进展 [J]. | 应用昆虫学报 , 2025 , 62 (03) : 535-548 . |
| MLA | 万文强 et al. "昆虫/植物的细胞自噬调控病毒侵染的研究进展" . | 应用昆虫学报 62 . 03 (2025) : 535-548 . |
| APA | 万文强 , 魏太云 , 陈倩 . 昆虫/植物的细胞自噬调控病毒侵染的研究进展 . | 应用昆虫学报 , 2025 , 62 (03) , 535-548 . |
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Co-infection with symbiotic viruses and arboviruses with synergistic effects in insect vectors are common in nature, but the underlying mechanism remains elusive. Here, we identify a novel symbiotic virus, leafhopper Recilia dorsalis bunyavirus (RdBV), which enhances the transmission efficiency of cytorhabdovirus rice stripe mosaic virus (RSMV, a plant rhabdovirus) in field. RSMV infection activates the expression of R. dorsalis E3 ubiquitin ligase Seven in absentia (RdSina), while RdBV infection suppresses its expression. We show that RdSina directly targets and mediates the degradation of RSMV phosphoprotein (P), thereby attenuating the formation of P-induced viroplasm that are crucial for viral replication. RdSina interacts with nonstructural protein NSs2 of RdBV but does not mediate its ubiquitination. However, NSs2 competes with RSMV P for binding to RdSina, thus neutralizing RdSina's ability in mediating P degradation. Furthermore, we find that the MYC transcription factor binds to the promoter sequences of RdSina, activating its transcription. However, NSs2 also directly binds to the same promoter sequences of RdSina and competitively suppresses MYC-activated RdSina transcription. Together, NSs2 obstructs the function of RdSina in mediating P degradation, ultimately promoting RSMV propagation in co-infected vectors. These findings elucidate how insect symbiotic viruses negatively regulate E3 ubiquitin ligases to benefit arbovirus transmission by co-infected insect vectors, which potentially is a common phenomenon in nature.
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| GB/T 7714 | Wang, Hui , Zhang, Jieting , Liu, Runfa et al. An insect symbiotic virus promotes the transmission of a phytoarbovirus via inhibiting E3 ubiquitin ligase Sina [J]. | PLOS PATHOGENS , 2025 , 21 (5) . |
| MLA | Wang, Hui et al. "An insect symbiotic virus promotes the transmission of a phytoarbovirus via inhibiting E3 ubiquitin ligase Sina" . | PLOS PATHOGENS 21 . 5 (2025) . |
| APA | Wang, Hui , Zhang, Jieting , Liu, Runfa , Li, You , Du, Yu , Wei, Taiyun . An insect symbiotic virus promotes the transmission of a phytoarbovirus via inhibiting E3 ubiquitin ligase Sina . | PLOS PATHOGENS , 2025 , 21 (5) . |
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本发明公开了一种柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用。本发明通过DcMucin‑like多克隆抗体的制备，首次验证了DcMucin‑like是柑橘木虱的唾液蛋白，并随木虱取食释放到植株组织中参与唾液鞘形成。本发明弥补了木虱唾液蛋白抗体制备和研究的空白，制备的柑橘木虱唾液蛋白多克隆抗体对DcMucin重组蛋白具有良好的特异性，基于该抗体免疫荧光标记观察木虱唾液鞘，为进一步研究DcMucin在黄龙病菌‑木虱‑柑橘三者互作中的生物学功能打下基础，为进一步获得柑橘木虱绿色防控特异性靶标奠定基础。
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| GB/T 7714 | 宾羽 , 魏太云 , 蒋丽琴 et al. 柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用 : CN202411432625.5[P]. | 2024-10-14 . |
| MLA | 宾羽 et al. "柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用" : CN202411432625.5. | 2024-10-14 . |
| APA | 宾羽 , 魏太云 , 蒋丽琴 , 李猷 . 柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用 : CN202411432625.5. | 2024-10-14 . |
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