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学者姓名：杜振国
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Abstract ：
<正>槟榔（Areca catechu L.）黄化病（yellow leaf disease, YLD）于1914年首次在印度报道，1981年在海南省屯昌县发现
Keyword ：
areca palm latent totivirus areca palm latent totivirus ORFan ORFan RNA-dependent RNA polymerase RNA-dependent RNA polymerase Totivirus Totivirus
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| GB/T 7714 | 肖思云 , 牛晓庆 , 徐钟天 et al. 海南槟榔潜隐单组分病毒1的分子进化特征分析 [J]. | 植物病理学报 , 2025 , 55 (03) : 550-554 . |
| MLA | 肖思云 et al. "海南槟榔潜隐单组分病毒1的分子进化特征分析" . | 植物病理学报 55 . 03 (2025) : 550-554 . |
| APA | 肖思云 , 牛晓庆 , 徐钟天 , 田雨菁 , 谢源 , 杜振国 et al. 海南槟榔潜隐单组分病毒1的分子进化特征分析 . | 植物病理学报 , 2025 , 55 (03) , 550-554 . |
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Tomato chlorosis virus (ToCV), first reported in Florida, USA, in 1998, has since emerged in multiple regions worldwide, posing a significant threat to global tomato production. However, its origin, migration patterns, and evolutionary history remain poorly understood. In this study, we used Bayesian phylogeographic analysis of coat protein gene sequences from 155 ToCV isolates to reconstruct its phylogeographic history. Our results show that ToCV evolves at a rate of 6.24 x 10-4 subs/site/year (95% credibility interval: 4.35 x 10-4-8.28 x 10-4), with the most recent common ancestor dating back to 1882. The maximum clade credibility (MCC) tree revealed three major clades, with Clade 1-whose most recent common ancestor dates to approximately 1975-comprising over 90% of the isolates. Although the exact origin of ToCV remains uncertain, we identified five distinct migration pathways: one from Europe to the Americas, one from Europe to South Asia, one from the Middle East to East Asia, one from East Asia to mainland China, and one from mainland China to Europe. These findings underscore the complex global spread of ToCV and suggest that multiple geographic areas have contributed to its ongoing evolution and dissemination.
Keyword ：
Bayesian phylodynamics Bayesian phylodynamics tomato chlorosis virus tomato chlorosis virus virus dispersal virus dispersal
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| GB/T 7714 | Wu, Kangcheng , Zhang, Shiwei , Huang, Wende et al. Phylogeographic History of Tomato Chlorosis Virus [J]. | VIRUSES-BASEL , 2025 , 17 (4) . |
| MLA | Wu, Kangcheng et al. "Phylogeographic History of Tomato Chlorosis Virus" . | VIRUSES-BASEL 17 . 4 (2025) . |
| APA | Wu, Kangcheng , Zhang, Shiwei , Huang, Wende , Du, Zhenguo , Gao, Fangluan , Guan, Xiayu . Phylogeographic History of Tomato Chlorosis Virus . | VIRUSES-BASEL , 2025 , 17 (4) . |
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The begomoviral V2 protein is known to be multifunctional, including its interaction with and inhibition of CYP1, a papain-like cysteine protease (PLCP). However, the effect of this interaction on viral pathogenicity remains unclear. Cotton leaf curl Multan virus (CLCuMuV), a typical monopartite begomovirus associated with a betasatellite, is one of the main pathogens responsible for cotton leaf curl disease. This study verifies the interaction between CLCuMuV V2 and NbCP15, a PLCP homologue in Nicotiana benthamiana. The results show that V2 can be cleaved by NbCP15 in vitro, with the N-terminal cleavage site located between the second and third amino acids. Using an Agrobacterium-mediated inoculation method, we investigated the influence of cleavage sites on viral pathogenicity. The findings indicate that mutation of the third amino acid in V2 (V2D3A) reduced the pathogenicity of both heterologous PVX and CLCuMuV. Additionally, the NbCP15 gene mutation in N. benthamiana (nbcp15) also resulted in reduced CLCuMuV pathogenicity. These results suggest that CLCuMuV V2 may promote viral infection through its interaction with plant PLCPs.
Keyword ：
begomovirus begomovirus cotton leaf curl Multan virus cotton leaf curl Multan virus papain-like cysteine protease papain-like cysteine protease V2 protein V2 protein
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| GB/T 7714 | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences [J]. | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
| MLA | Zhang, Jie et al. "Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences" . | MOLECULAR PLANT PATHOLOGY 26 . 1 (2025) . |
| APA | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai , Li, Dingshan , Liu, Shunmin , Du, Zhenguo et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences . | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
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【目的】明确福建省茶树主要病毒种类和分布情况，并建立同时快速检测多种病毒的多重PCR检测技术。【方法】2019—2023年，从福建省福州、南平、宁德、泉州、漳州、厦门、三明、莆田和龙岩9个地市区采集具有褪绿、皱缩和坏死等疑似病毒感染症状的茶树样品1 869份，采用高通量测序技术结合PCR和RT-PCR检测的方法对茶树病毒病的病原进行鉴定，并将PCR扩增获得的特异性目的片段进行克隆和测序，对获得的序列进行系统发育分析；同时，根据GenBank上已报道的病毒序列设计特异性引物，通过退火温度、引物浓度、循环数等反应条件和反应程序的优化，建立同时检测福建茶树主要病毒的多重PCR检测技术，并测定该技术的特异性、灵敏度及实际应用效果。【结果】从所采集的茶树病样上检出3种病毒，按检出率从高到低依次为油茶双生病毒（oil tea associated geminivirus,OTaGV）(48.90%)、茶树坏死环斑病毒（tea plant necrotic ring blotch virus,TPNRBV）(26.75%)和茶树潜隐病毒1(camellia cryptic virus 1,CCV1)(17.98%)；在检出病毒的1 258份样品中，有807份为OTaGV、TPNRBV或CCV1单独侵染，检出率分别为37.20%、21.38%和5.56%；其余451份样品为2种或3种病毒复合侵染，复合侵染检出率达35.85%,OTaGV+CCV1、TPNRBV+CCV1、TPNRBV+OTaGV、OTaGV+CCV1+TPNRBV 4种类型复合侵染检出率分别为17.49%、0.40%、14.71%、3.26%。在地理分布上，OTaGV在福州、南平、宁德、泉州、漳州、厦门、三明、莆田和龙岩9个地区均有分布，其中漳州OTaGV检出率最高，为96.77%;CCV1在福州、南平、宁德、泉州、漳州、三明、莆田和龙岩8个地区均有分布，其中三明CCV1检出率最高，为66.00%;TPNRBV在福州、南平、宁德、泉州和漳州5个地区均有分布，其中泉州TPNRBV检出率最高，为79.13%；在福建省9个地区中，厦门地区仅检出OTaGV，三明、莆田和龙岩地区检出OTaGV和CCV1，其他5个地区同时检出OTaGV、TPNRBV和CCV1；福州、南平、宁德、泉州、漳州、三明、莆田7个地区检测到病毒复合侵染，其中漳州地区病毒复合侵染检出率最高（85.00...
Keyword ：
分子检测 分子检测 多重PCR 多重PCR 病原鉴定 病原鉴定 福建省 福建省 茶树病毒 茶树病毒
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| GB/T 7714 | 陈细红 , 蔡伟 , 虞赟 et al. 福建省茶树病毒种类鉴定及多重PCR检测技术的建立 [J]. | 中国农业科学 , 2024 , 57 (04) : 698-710 . |
| MLA | 陈细红 et al. "福建省茶树病毒种类鉴定及多重PCR检测技术的建立" . | 中国农业科学 57 . 04 (2024) : 698-710 . |
| APA | 陈细红 , 蔡伟 , 虞赟 , 李敏 , 王念武 , 杜振国 et al. 福建省茶树病毒种类鉴定及多重PCR检测技术的建立 . | 中国农业科学 , 2024 , 57 (04) , 698-710 . |
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The genome of a monopartite begomovirus, or the DNA-A component of a bipartite begomovirus, typically encodes six proteins: two on the viral strand (AV1/V1 and AV2/V2) and four on the complementary strand (AC1/ C1, AC2/C2, AC3/C3, AC4/C4). Recent studies, however, have identified additional begomoviral proteins with various functions. This paper reports that euphorbia leaf curl virus (EuLCV), a monopartite begomovirus, encodes a seventh protein, C5. Promoter activity of the upstream fragment of the EuLCV C5 gene was shown using a GUS expression vector. EuLCV C5 also enhanced the pathogenicity and accumulation of potato virus X (PVX) in Nicotiana benthamiana. Localization studies revealed that EuLCV C5 localizes to the cytoplasm and nucleus, forming granular structures on the cell membrane. Additionally, C5 acts as a post-transcriptional gene silencing (PTGS) suppressor. A C5 deletion mutant of EuLCV (EuLCV-Delta C5) exhibited reduced pathogenicity and viral accumulation compared to wild-type EuLCV in N. benthamiana.
Keyword ：
C5 protein C5 protein Euphorbia leaf curl virus Euphorbia leaf curl virus Pathogenicity determinant Pathogenicity determinant Post-transcriptional gene silencing Post-transcriptional gene silencing
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| GB/T 7714 | Zhang, Jie , Yuan, Linkai , Li, Dingshan et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor [J]. | VIROLOGY , 2024 , 600 . |
| MLA | Zhang, Jie et al. "The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor" . | VIROLOGY 600 (2024) . |
| APA | Zhang, Jie , Yuan, Linkai , Li, Dingshan , Yang, Xueying , Li, Jingyuan , Wu, Zujian et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor . | VIROLOGY , 2024 , 600 . |
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Yellow leaf disease (YLD) poses a significant challenge to areca palm cultivation, yet its etiology remains uncertain. During our investigation of YLD-affected areca palm plants, transcriptome sequencing revealed an RNA contig exhibiting striking similarities to the RNA-dependent RNA polymerase (RdRp) of ormycoviruses. Subsequent gene cloning techniques yielded the full-length sequence of this RNA, potentially representing either the complete or partial genome of a hitherto unidentified ormycovirus, tentatively named areca palm yellow leaf-associated ormycovirus (APYLaOMV). RT-PCR detection found that APYLaOMV is present in over 30% of YLD-affected areca palm samples but is absent in healthy ones, suggesting a potential link between APYLaOMV and YLD. In summary, these data could be valuable in understanding the etiology of YLD in areca palms.
Keyword ：
areca palm areca palm ORFan ORFan ormycovirus ormycovirus RNA-dependent RNA polymerase RNA-dependent RNA polymerase
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| GB/T 7714 | Niu, Xiaoqing , Xu, Zhongtian , Tian, Yujing et al. A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm [J]. | FORESTS , 2024 , 15 (6) . |
| MLA | Niu, Xiaoqing et al. "A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm" . | FORESTS 15 . 6 (2024) . |
| APA | Niu, Xiaoqing , Xu, Zhongtian , Tian, Yujing , Xiao, Siyun , Xie, Yuan , Du, Zhenguo et al. A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm . | FORESTS , 2024 , 15 (6) . |
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Tobacco curly shoot virus (TbCSV), a begomovirus, causes significant economic losses in tobacco and tomato crops across East, Southeast, and South Asia. Despite its agricultural importance, the evolutionary dynamics and emergence process of TbCSV remain poorly understood. This study analyzed the phylodynamics of TbCSV by examining its nucleotide sequences of the coat protein (CP) gene collected between 2000 and 2022. Using various combinations of priors, Bayes factor comparisons identified heterochronous datasets (3 x 100 million chains) generated from a strict molecular clock and Bayesian skyline tree priors as the most robust. The mean substitution rate of the CP gene was estimated at 6.50 x 10-4 substitutions/site/year (95% credibility interval: 4.74 x 10-4-8.50 x 10-4). TbCSV was inferred to have diverged around 1920 CE (95% credibility interval: 1887-1952), with its most probable origin in South Asia. These findings provide valuable insights for the phylogeography and evolutionary dynamics of TbCSV, and contribute to a broader understanding of begomovirus epidemiology.
Keyword ：
Bayesian phylodynamics Bayesian phylodynamics Begomovirus Begomovirus substitution rate substitution rate temporal signal temporal signal tobacco curly shoot virus tobacco curly shoot virus
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| GB/T 7714 | Long, Xingxiu , Zhang, Shiwei , Shen, Jianguo et al. Phylogeography and Evolutionary Dynamics of Tobacco Curly Shoot Virus [J]. | VIRUSES-BASEL , 2024 , 16 (12) . |
| MLA | Long, Xingxiu et al. "Phylogeography and Evolutionary Dynamics of Tobacco Curly Shoot Virus" . | VIRUSES-BASEL 16 . 12 (2024) . |
| APA | Long, Xingxiu , Zhang, Shiwei , Shen, Jianguo , Du, Zhenguo , Gao, Fangluan . Phylogeography and Evolutionary Dynamics of Tobacco Curly Shoot Virus . | VIRUSES-BASEL , 2024 , 16 (12) . |
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Tea plant necrotic ring blotch virus (TPNRBV) is a kitavirus that poses a serious threat to the cultivation of tea, one of the most economically important plants in many Asian countries. However, the occurrence and genetic variability of this virus remain poorly understood. This study investigated the occurrence of TPNRBV in Fujian Province, China, one of the largest tea producers in the country, and determined the complete genome of 10 new TPNRBV isolates. The results revealed that TPNRBV is widespread in Fujian. The nucleotide diversity values for the RNA1-4 segments of TPNRBV were found to be 0.027, 0.016, 0.027, and 0.014, respectively. Among the seven proteins of TPNRBV, P22 was the least conserved, while MP was the most conserved. The 5 ' termini of the genomic segments of TPNRBV commonly possessed a sequence of 5 '-AATTACGA-3 ' (RNA1-3) or 5 '-ATTAACGA-3 ' (RNA4). Furthermore, the 3 ' non-coding region of TPNRBV RNA4 exhibited significant length variation due to frequent deletion/insertion mutations. Recombination and purifying selection likely played important roles in shaping the genetic structure of TPNRBV. These findings provide a snapshot of the epidemiology and genetic structure of TPNRBV, offering valuable information for the development of targeted strategies to control and manage TPNRBV in tea cultivation.
Keyword ：
epidemiology epidemiology evolution evolution genetic variability genetic variability kitavirus kitavirus tea plant necrotic ring blotch virus tea plant necrotic ring blotch virus
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| GB/T 7714 | Chen, Xihong , Shen, Jianguo , Li, Min et al. The Occurrence and Genetic Variability of Tea Plant Necrotic Ring Blotch Virus in Fujian Province, China [J]. | FORESTS , 2023 , 14 (9) . |
| MLA | Chen, Xihong et al. "The Occurrence and Genetic Variability of Tea Plant Necrotic Ring Blotch Virus in Fujian Province, China" . | FORESTS 14 . 9 (2023) . |
| APA | Chen, Xihong , Shen, Jianguo , Li, Min , Gao, Yujie , Du, Zhenguo , Gao, Fangluan . The Occurrence and Genetic Variability of Tea Plant Necrotic Ring Blotch Virus in Fujian Province, China . | FORESTS , 2023 , 14 (9) . |
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Many geminiviruses, including members of the genus Begomovirus, produce a protein known as C4 or AC4. Whereas C4/AC4 typically consists of more than 80 amino acid residues, a few are much shorter. The significance of these shorter C4/AC4 proteins in viral infection and why the virus maintains their abbreviated length is not yet understood. The AC4 of the begomovirus Tomato leaf curl Hsinchu virus contains only 65 amino acids, but it extends to 96 amino acids when the natural termination codon is replaced with a normal codon. We discovered that both interrupting and extending AC4 were harmful to tomato leaf curl Hsinchu virus (ToLCHsV). The extended AC4 (EAC4) also showed a reduced ability to promote the infection of the heterologous virus Potato virus X than the wild-type AC4. When the wild-type AC4 was fused with yellow fluorescent protein (AC4-YFP), it was predominantly found in chloroplasts, whereas EAC4-YFP was mainly localized to the cell periphery. These results suggest that ToLCHsV's AC4 protein is important for viral infection, and the virus may benefit from the abbreviated length, because it may lead to chloroplast localization.Copyright (c) 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Keyword ：
AC4 AC4 begomovirus begomovirus geminivirus geminivirus tomato leaf curl Hsinchu virus tomato leaf curl Hsinchu virus
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| GB/T 7714 | Zhang, Wenwen , Liu, Shunmin , Xie, Guohui et al. Size Restriction Is Required for Proper Functioning of a Bipartite Begomovirus AC4 Protein [J]. | MOLECULAR PLANT-MICROBE INTERACTIONS , 2023 , 36 (12) : 774-778 . |
| MLA | Zhang, Wenwen et al. "Size Restriction Is Required for Proper Functioning of a Bipartite Begomovirus AC4 Protein" . | MOLECULAR PLANT-MICROBE INTERACTIONS 36 . 12 (2023) : 774-778 . |
| APA | Zhang, Wenwen , Liu, Shunmin , Xie, Guohui , Li, Xiuyu , Zhai, Yingying , Lin, Wenzhong et al. Size Restriction Is Required for Proper Functioning of a Bipartite Begomovirus AC4 Protein . | MOLECULAR PLANT-MICROBE INTERACTIONS , 2023 , 36 (12) , 774-778 . |
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Ralstonia solanacearum is a devastating bacterial pathogen responsible for bacterial wilt in various crops. It employs the Type III Secretion System (T3SS) to deliver effectors known as R. solanacearum injected proteins (Rips) into host plant cells. These Rips play a critical role in the pathogenicity of the bacterium, yet their mechanisms of action remain incompletely understood. In this study, we screened 13 Rips from R. solanacearum strain FJ1003 and discovered that RipAW and RipAU possess the ability to suppress RNA silencing, an essential gene regulatory mechanism in plants. Moreover, we demonstrated heightened susceptibility to R. solanacearum and potato virus X in transgenic Nicotiana benthamiana overexpressing RipAW. Silencing of Dicer-like 1 (DCL1) or RNA-dependent RNA polymerase 6 (RDR6) promoted the infection of R. solanacearum in N. benthamiana. Arabidopsis with defects in Argonaute 1 (AGO1), DCL1 or RDR6 also showed increased susceptibility to R. solanacearum compared to wildtype Arabidopsis. These findings collectively suggest a scenario in which certain Rips interfere with host plant RNA silencing, and RNA silencing suppression may be one of the mechanisms employed by Rips to promote virulence.
Keyword ：
RNA silencing RNA silencing RNA silencing suppressor RNA silencing suppressor R. solanacearum injected proteins R. solanacearum injected proteins
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| GB/T 7714 | Qiu, Ping , Xu, Yixing , Chen, Lihong et al. Some Type III effectors of Ralstonia solanacearum may suppress host RNA silencing for function [J]. | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY , 2023 , 128 . |
| MLA | Qiu, Ping et al. "Some Type III effectors of Ralstonia solanacearum may suppress host RNA silencing for function" . | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY 128 (2023) . |
| APA | Qiu, Ping , Xu, Yixing , Chen, Lihong , Lin, Wenzhong , Zou, Huasong , Wu, Zujian et al. Some Type III effectors of Ralstonia solanacearum may suppress host RNA silencing for function . | PHYSIOLOGICAL AND MOLECULAR PLANT PATHOLOGY , 2023 , 128 . |
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