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学者姓名：杜振国
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Abstract ：
The begomoviral V2 protein is known to be multifunctional, including its interaction with and inhibition of CYP1, a papain-like cysteine protease (PLCP). However, the effect of this interaction on viral pathogenicity remains unclear. Cotton leaf curl Multan virus (CLCuMuV), a typical monopartite begomovirus associated with a betasatellite, is one of the main pathogens responsible for cotton leaf curl disease. This study verifies the interaction between CLCuMuV V2 and NbCP15, a PLCP homologue in Nicotiana benthamiana. The results show that V2 can be cleaved by NbCP15 in vitro, with the N-terminal cleavage site located between the second and third amino acids. Using an Agrobacterium-mediated inoculation method, we investigated the influence of cleavage sites on viral pathogenicity. The findings indicate that mutation of the third amino acid in V2 (V2D3A) reduced the pathogenicity of both heterologous PVX and CLCuMuV. Additionally, the NbCP15 gene mutation in N. benthamiana (nbcp15) also resulted in reduced CLCuMuV pathogenicity. These results suggest that CLCuMuV V2 may promote viral infection through its interaction with plant PLCPs.
Keyword ：
begomovirus begomovirus cotton leaf curl Multan virus cotton leaf curl Multan virus papain-like cysteine protease papain-like cysteine protease V2 protein V2 protein
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| GB/T 7714 | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences [J]. | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
| MLA | Zhang, Jie et al. "Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences" . | MOLECULAR PLANT PATHOLOGY 26 . 1 (2025) . |
| APA | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai , Li, Dingshan , Liu, Shunmin , Du, Zhenguo et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences . | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
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Tomato chlorosis virus (ToCV), first reported in Florida, USA, in 1998, has since emerged in multiple regions worldwide, posing a significant threat to global tomato production. However, its origin, migration patterns, and evolutionary history remain poorly understood. In this study, we used Bayesian phylogeographic analysis of coat protein gene sequences from 155 ToCV isolates to reconstruct its phylogeographic history. Our results show that ToCV evolves at a rate of 6.24 x 10-4 subs/site/year (95% credibility interval: 4.35 x 10-4-8.28 x 10-4), with the most recent common ancestor dating back to 1882. The maximum clade credibility (MCC) tree revealed three major clades, with Clade 1-whose most recent common ancestor dates to approximately 1975-comprising over 90% of the isolates. Although the exact origin of ToCV remains uncertain, we identified five distinct migration pathways: one from Europe to the Americas, one from Europe to South Asia, one from the Middle East to East Asia, one from East Asia to mainland China, and one from mainland China to Europe. These findings underscore the complex global spread of ToCV and suggest that multiple geographic areas have contributed to its ongoing evolution and dissemination.
Keyword ：
Bayesian phylodynamics Bayesian phylodynamics tomato chlorosis virus tomato chlorosis virus virus dispersal virus dispersal
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| GB/T 7714 | Wu, Kangcheng , Zhang, Shiwei , Huang, Wende et al. Phylogeographic History of Tomato Chlorosis Virus [J]. | VIRUSES-BASEL , 2025 , 17 (4) . |
| MLA | Wu, Kangcheng et al. "Phylogeographic History of Tomato Chlorosis Virus" . | VIRUSES-BASEL 17 . 4 (2025) . |
| APA | Wu, Kangcheng , Zhang, Shiwei , Huang, Wende , Du, Zhenguo , Gao, Fangluan , Guan, Xiayu . Phylogeographic History of Tomato Chlorosis Virus . | VIRUSES-BASEL , 2025 , 17 (4) . |
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Abstract ：
<正>槟榔（Areca catechu L.）黄化病（yellow leaf disease, YLD）于1914年首次在印度报道，1981年在海南省屯昌县发现
Keyword ：
areca palm latent totivirus areca palm latent totivirus ORFan ORFan RNA-dependent RNA polymerase RNA-dependent RNA polymerase Totivirus Totivirus
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| GB/T 7714 | 肖思云 , 牛晓庆 , 徐钟天 et al. 海南槟榔潜隐单组分病毒1的分子进化特征分析 [J]. | 植物病理学报 , 2025 , 55 (03) : 550-554 . |
| MLA | 肖思云 et al. "海南槟榔潜隐单组分病毒1的分子进化特征分析" . | 植物病理学报 55 . 03 (2025) : 550-554 . |
| APA | 肖思云 , 牛晓庆 , 徐钟天 , 田雨菁 , 谢源 , 杜振国 et al. 海南槟榔潜隐单组分病毒1的分子进化特征分析 . | 植物病理学报 , 2025 , 55 (03) , 550-554 . |
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【目的】明确福建省茶树主要病毒种类和分布情况，并建立同时快速检测多种病毒的多重PCR检测技术。【方法】2019—2023年，从福建省福州、南平、宁德、泉州、漳州、厦门、三明、莆田和龙岩9个地市区采集具有褪绿、皱缩和坏死等疑似病毒感染症状的茶树样品1 869份，采用高通量测序技术结合PCR和RT-PCR检测的方法对茶树病毒病的病原进行鉴定，并将PCR扩增获得的特异性目的片段进行克隆和测序，对获得的序列进行系统发育分析；同时，根据GenBank上已报道的病毒序列设计特异性引物，通过退火温度、引物浓度、循环数等反应条件和反应程序的优化，建立同时检测福建茶树主要病毒的多重PCR检测技术，并测定该技术的特异性、灵敏度及实际应用效果。【结果】从所采集的茶树病样上检出3种病毒，按检出率从高到低依次为油茶双生病毒（oil tea associated geminivirus,OTaGV）(48.90%)、茶树坏死环斑病毒（tea plant necrotic ring blotch virus,TPNRBV）(26.75%)和茶树潜隐病毒1(camellia cryptic virus 1,CCV1)(17.98%)；在检出病毒的1 258份样品中，有807份为OTaGV、TPNRBV或CCV1单独侵染，检出率分别为37.20%、21.38%和5.56%；其余451份样品为2种或3种病毒复合侵染，复合侵染检出率达35.85%,OTaGV+CCV1、TPNRBV+CCV1、TPNRBV+OTaGV、OTaGV+CCV1+TPNRBV 4种类型复合侵染检出率分别为17.49%、0.40%、14.71%、3.26%。在地理分布上，OTaGV在福州、南平、宁德、泉州、漳州、厦门、三明、莆田和龙岩9个地区均有分布，其中漳州OTaGV检出率最高，为96.77%;CCV1在福州、南平、宁德、泉州、漳州、三明、莆田和龙岩8个地区均有分布，其中三明CCV1检出率最高，为66.00%;TPNRBV在福州、南平、宁德、泉州和漳州5个地区均有分布，其中泉州TPNRBV检出率最高，为79.13%；在福建省9个地区中，厦门地区仅检出OTaGV，三明、莆田和龙岩地区检出OTaGV和CCV1，其他5个地区同时检出OTaGV、TPNRBV和CCV1；福州、南平、宁德、泉州、漳州、三明、莆田7个地区检测到病毒复合侵染，其中漳州地区病毒复合侵染检出率最高（85.00...
Keyword ：
分子检测 分子检测 多重PCR 多重PCR 病原鉴定 病原鉴定 福建省 福建省 茶树病毒 茶树病毒
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| GB/T 7714 | 陈细红 , 蔡伟 , 虞赟 et al. 福建省茶树病毒种类鉴定及多重PCR检测技术的建立 [J]. | 中国农业科学 , 2024 , 57 (04) : 698-710 . |
| MLA | 陈细红 et al. "福建省茶树病毒种类鉴定及多重PCR检测技术的建立" . | 中国农业科学 57 . 04 (2024) : 698-710 . |
| APA | 陈细红 , 蔡伟 , 虞赟 , 李敏 , 王念武 , 杜振国 et al. 福建省茶树病毒种类鉴定及多重PCR检测技术的建立 . | 中国农业科学 , 2024 , 57 (04) , 698-710 . |
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Yellow leaf disease (YLD) poses a significant challenge to areca palm cultivation, yet its etiology remains uncertain. During our investigation of YLD-affected areca palm plants, transcriptome sequencing revealed an RNA contig exhibiting striking similarities to the RNA-dependent RNA polymerase (RdRp) of ormycoviruses. Subsequent gene cloning techniques yielded the full-length sequence of this RNA, potentially representing either the complete or partial genome of a hitherto unidentified ormycovirus, tentatively named areca palm yellow leaf-associated ormycovirus (APYLaOMV). RT-PCR detection found that APYLaOMV is present in over 30% of YLD-affected areca palm samples but is absent in healthy ones, suggesting a potential link between APYLaOMV and YLD. In summary, these data could be valuable in understanding the etiology of YLD in areca palms.
Keyword ：
areca palm areca palm ORFan ORFan ormycovirus ormycovirus RNA-dependent RNA polymerase RNA-dependent RNA polymerase
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| GB/T 7714 | Niu, Xiaoqing , Xu, Zhongtian , Tian, Yujing et al. A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm [J]. | FORESTS , 2024 , 15 (6) . |
| MLA | Niu, Xiaoqing et al. "A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm" . | FORESTS 15 . 6 (2024) . |
| APA | Niu, Xiaoqing , Xu, Zhongtian , Tian, Yujing , Xiao, Siyun , Xie, Yuan , Du, Zhenguo et al. A Putative Ormycovirus That Possibly Contributes to the Yellow Leaf Disease of Areca Palm . | FORESTS , 2024 , 15 (6) . |
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The genome of a monopartite begomovirus, or the DNA-A component of a bipartite begomovirus, typically encodes six proteins: two on the viral strand (AV1/V1 and AV2/V2) and four on the complementary strand (AC1/ C1, AC2/C2, AC3/C3, AC4/C4). Recent studies, however, have identified additional begomoviral proteins with various functions. This paper reports that euphorbia leaf curl virus (EuLCV), a monopartite begomovirus, encodes a seventh protein, C5. Promoter activity of the upstream fragment of the EuLCV C5 gene was shown using a GUS expression vector. EuLCV C5 also enhanced the pathogenicity and accumulation of potato virus X (PVX) in Nicotiana benthamiana. Localization studies revealed that EuLCV C5 localizes to the cytoplasm and nucleus, forming granular structures on the cell membrane. Additionally, C5 acts as a post-transcriptional gene silencing (PTGS) suppressor. A C5 deletion mutant of EuLCV (EuLCV-Delta C5) exhibited reduced pathogenicity and viral accumulation compared to wild-type EuLCV in N. benthamiana.
Keyword ：
C5 protein C5 protein Euphorbia leaf curl virus Euphorbia leaf curl virus Pathogenicity determinant Pathogenicity determinant Post-transcriptional gene silencing Post-transcriptional gene silencing
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| GB/T 7714 | Zhang, Jie , Yuan, Linkai , Li, Dingshan et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor [J]. | VIROLOGY , 2024 , 600 . |
| MLA | Zhang, Jie et al. "The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor" . | VIROLOGY 600 (2024) . |
| APA | Zhang, Jie , Yuan, Linkai , Li, Dingshan , Yang, Xueying , Li, Jingyuan , Wu, Zujian et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor . | VIROLOGY , 2024 , 600 . |
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Tobacco curly shoot virus (TbCSV), a begomovirus, causes significant economic losses in tobacco and tomato crops across East, Southeast, and South Asia. Despite its agricultural importance, the evolutionary dynamics and emergence process of TbCSV remain poorly understood. This study analyzed the phylodynamics of TbCSV by examining its nucleotide sequences of the coat protein (CP) gene collected between 2000 and 2022. Using various combinations of priors, Bayes factor comparisons identified heterochronous datasets (3 x 100 million chains) generated from a strict molecular clock and Bayesian skyline tree priors as the most robust. The mean substitution rate of the CP gene was estimated at 6.50 x 10-4 substitutions/site/year (95% credibility interval: 4.74 x 10-4-8.50 x 10-4). TbCSV was inferred to have diverged around 1920 CE (95% credibility interval: 1887-1952), with its most probable origin in South Asia. These findings provide valuable insights for the phylogeography and evolutionary dynamics of TbCSV, and contribute to a broader understanding of begomovirus epidemiology.
Keyword ：
Bayesian phylodynamics Bayesian phylodynamics Begomovirus Begomovirus substitution rate substitution rate temporal signal temporal signal tobacco curly shoot virus tobacco curly shoot virus
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| GB/T 7714 | Long, Xingxiu , Zhang, Shiwei , Shen, Jianguo et al. Phylogeography and Evolutionary Dynamics of Tobacco Curly Shoot Virus [J]. | VIRUSES-BASEL , 2024 , 16 (12) . |
| MLA | Long, Xingxiu et al. "Phylogeography and Evolutionary Dynamics of Tobacco Curly Shoot Virus" . | VIRUSES-BASEL 16 . 12 (2024) . |
| APA | Long, Xingxiu , Zhang, Shiwei , Shen, Jianguo , Du, Zhenguo , Gao, Fangluan . Phylogeography and Evolutionary Dynamics of Tobacco Curly Shoot Virus . | VIRUSES-BASEL , 2024 , 16 (12) . |
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单组分双生病毒V2蛋白在病毒侵染过程中行使重要功能，而双组分双生病毒AV2蛋白功能的研究相对较少。为揭示双组分双生病毒新竹番茄曲叶病毒(Tomato leaf curl Hsinchu virus, ToLCHsV) AV2蛋白功能，本研究利用邻近依赖的生物素鉴定(proximity-dependent biotin identification, BioID)-质谱联用技术，在本氏烟(Nicotiana benthamiana)中初步筛选出与AV2邻近的多个寄主蛋白。利用酵母双杂交(yeast two hybrid, YTH)和双分子荧光互补(bimolecular fluorescence complementation, BiFC)验证了本氏烟甲羟戊酸激酶a(N. benthamiana mevalonate kinase a, NbMVKa)和ToLCHsV-AV2存在互作。利用烟草脆裂病毒(Tobacco rattle virus, TRV)诱导的基因沉默技术，将ToLCHsV侵染性克隆接种NbMVKa沉默的本氏烟，接种10 d后，NbMVKa沉默的本氏烟症状不明显，而未沉默植株表现叶片卷曲，同时利用Southern blot分析发现，沉默植株的病毒DNA积累量相对减少；接种20 d后，NbMVKa沉默的本氏烟和未沉默植株症状基本一致。结果表明，NbMVKa可能通过与ToLCHsV-AV2互作而对病毒致病起正调控的作用。本研究结果为进一步阐明双组分双生病毒AV2蛋白功能提供理论依据。
Keyword ：
AV2蛋白 AV2蛋白 新竹番茄曲叶病毒(ToLCHsV) 新竹番茄曲叶病毒(ToLCHsV) 本氏烟甲羟戊酸激酶a(NbMVKa) 本氏烟甲羟戊酸激酶a(NbMVKa) 邻近依赖的生物素鉴定(BioID) 邻近依赖的生物素鉴定(BioID)
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| GB/T 7714 | 李丁山 , 韩星 , 林文忠 et al. NbMVKa与新竹番茄曲叶病毒AV2蛋白互作对病毒致病性的影响 [J]. | 农业生物技术学报 , 2023 , 31 (09) : 1924-1934 . |
| MLA | 李丁山 et al. "NbMVKa与新竹番茄曲叶病毒AV2蛋白互作对病毒致病性的影响" . | 农业生物技术学报 31 . 09 (2023) : 1924-1934 . |
| APA | 李丁山 , 韩星 , 林文忠 , 查晴辰 , 张文文 , 杜振国 et al. NbMVKa与新竹番茄曲叶病毒AV2蛋白互作对病毒致病性的影响 . | 农业生物技术学报 , 2023 , 31 (09) , 1924-1934 . |
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近年来，广东番木瓜曲叶病毒(Papaya leaf curl Guangdong virus, PaLCuGDV)和一品红曲叶病毒(Euphorbia leaf curl virus, EuLCV)经常在百香果(Passiflora edulis)上被鉴定。本研究从福建地区表现典型双生病毒危害症状的百香果上扩增得到该2种病毒的全长序列，分别为2 732和2 745 bp；为明确PaLCuGDV和EuLCV的致病性，利用同源重组技术构建了2种病毒的侵染性克隆，通过农杆菌(Agrobacterium tumefaciens)介导法接种本氏烟(Nicotiana benthamiana)，结果发现，接种EuLCV的本氏烟表现出明显矮化且顶端叶片畸形卷曲症状，受病害危害明显重于接种PaLCuGDV，后者仅表现新叶稍微卷曲和叶片黄化；2种病毒混合侵染，受病害危害重于单独接种EuLCV或PaLCuGDV。Southern blot结果显示，PaLCuGDV和EuLCV单独及混合接种的烟草均有较高的病毒积累量。本研究首次构建了PaLCuGDV的侵染性克隆，并发现EuLCV和PaLCuGDV复合侵染会加重病害症状。本研究为解析PaLCuGDV和EuLCV的致病机制提供参考。
Keyword ：
一品红曲叶病毒(EuLCV) 一品红曲叶病毒(EuLCV) 侵染性克隆 侵染性克隆 广东番木瓜曲叶病毒(PaLCuGDV) 广东番木瓜曲叶病毒(PaLCuGDV) 百香果 百香果
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| GB/T 7714 | 李景远 , 林文忠 , 李丁山 et al. 广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析 [J]. | 农业生物技术学报 , 2023 , 31 (09) : 1935-1943 . |
| MLA | 李景远 et al. "广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析" . | 农业生物技术学报 31 . 09 (2023) : 1935-1943 . |
| APA | 李景远 , 林文忠 , 李丁山 , 马崇欢 , 吴祖建 , 杜振国 et al. 广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析 . | 农业生物技术学报 , 2023 , 31 (09) , 1935-1943 . |
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为明确福建茶树潜隐病毒(camellia cryptic virus 1, CCV1)发生情况，探明其全基因组序列特征及其与油茶潜隐病毒1(camellia oleifera cryptic virus 1, CoCV1)的关系，本研究通过RT-PCR方法对采自福建7个茶树种植区疑似感染CCV1的叶片进行检测，并随机选取18个CCV1分离物，经扩增、克隆获得全基因组序列，对其序列特征和系统发育关系进行分析。结果显示，431份样品中共有94份样品检测出CCV1,检出率为21.81%。测定获得的18个CCV1分离物dsRNA1、dsRNA2和dsRNA3的核苷酸序列全长分别为1 722-1 725 bp、1 504-1 506 bp和1 497-1 499 bp。克隆获得的RdRp、CP和mCP的氨基酸序列与CCV1代表分离物Won(GenBank登录号MH898482-MH898484)和CoCV1代表分离物PXCS5(GenBank登录号MH814756-MH814758)的一致性值分别≥91%、≥84%和≥90%,超过了δ-分体病毒属(Deltapartitivirus)中种的界定标准阈值。进一步的系统发育分析结果显示，所有CCV1分离物与CoCV1分离物以高置信值相聚成簇，证实CCV1和CoCV1为Deltapartitivirus属的同一种病毒。
Keyword ：
δ-分体病毒属 δ-分体病毒属 分子特征 分子特征 油茶潜隐病毒1 油茶潜隐病毒1 系统发育分析 系统发育分析 茶树潜隐病毒1 茶树潜隐病毒1
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| GB/T 7714 | 许瑜婷 , 丁莹 , 沈建国 et al. 福建茶树潜隐病毒1的检测及全基因组序列特征 [J]. | 植物病理学报 , 2023 , 53 (03) : 386-394 . |
| MLA | 许瑜婷 et al. "福建茶树潜隐病毒1的检测及全基因组序列特征" . | 植物病理学报 53 . 03 (2023) : 386-394 . |
| APA | 许瑜婷 , 丁莹 , 沈建国 , 陈细红 , 章淑玲 , 杜振国 et al. 福建茶树潜隐病毒1的检测及全基因组序列特征 . | 植物病理学报 , 2023 , 53 (03) , 386-394 . |
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