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学者姓名：卓涛
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Abstract ：
Xanthomonas citri subsp. citri (Xcc), the causal agent of citrus canker, elicits canker symptoms in citrus plants because of the transcriptional activator-like (TAL) effector PthA4, which activates the expression of the citrus susceptibility gene CsLOB1. This study reports the regulation of the putative carbohydrate-binding protein gene Cs9g12620 by PthA4-mediated induction of CsLOB1 during Xcc infection. We found that the transcription of Cs9g12620 was induced by infection with Xcc in a PthA4-dependent manner. Even though it specifically bound to a putative TAL effector-binding element in the Cs9g12620 promoter, PthA4 exerted a suppressive effect on the promoter activity. In contrast, CsLOB1 bound to the Cs9g12620 promoter to activate its expression. The silencing of CsLOB1 significantly reduced the level of expression of Cs9g12620, which demonstrated that Cs9g12620 was directly regulated by CsLOB1. Intriguingly, PhtA4 interacted with CsLOB1 and exerted feedback control that suppressed the induction of expression of Cs9g12620 by CsLOB1. Transient overexpression and gene silencing revealed that Cs9g12620 was required for the optimal development of canker symptoms. These results support the hypothesis that the expression of Cs9g12620 is dynamically directed by PthA4 for canker formation through the PthA4-mediated induction of CsLOB1.
Keyword ：
citrus bacterial canker citrus bacterial canker Citrus sinensis Citrus sinensis Other Other Xanthomonas citri subsp. citri Xanthomonas citri subsp. citri
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| GB/T 7714 | Chen, Xinyu , Zou, Huasong , Zhuo, Tao et al. Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation [J]. | ELIFE , 2024 , 13 . |
| MLA | Chen, Xinyu et al. "Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation" . | ELIFE 13 (2024) . |
| APA | Chen, Xinyu , Zou, Huasong , Zhuo, Tao , Rou, Wei , Wu, Wei , Fan, Xiaojing . Xanthomonas citri subsp. citri type III effector PthA4 directs the dynamical expression of a putative citrus carbohydrate-binding protein gene for canker formation . | ELIFE , 2024 , 13 . |
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Background LysR-type transcriptional regulators (LTTRs) are one of the largest families of regulators in prokaryotic organism, which help the bacterium adapt to diverse conditions by controlling a wide array of regulons, encompassing genes responsible for nitrogen and carbon fixation, oxidative stress response, bacterial virulence, and the breakdown of diverse compounds. Ralstonia solanacearum strain GMI1000 possesses 80 LTTR genes, yet the precise roles and functional contributions of only three of these LTTRs have been conclusively established among the total. In this work, our group reveal a novel LTTR member LysR7 (RS_RS02375) that exerts multiple regulatory roles in motility, carbon metabolism and virulence. Results In this investigation, an in-frame deletion mutant Delta lysR7 and a complemented strain C Delta lysR7 were prepared. The mutant Delta lysR7 had increased swimming motility on semi-soft medium and showed a reduced replication rate in nutrient-rich medium and in planta. Moreover, Delta lysR7 was unable to grow on nutrient-limited medium, supplemented with galactose as a single carbon resource. RT-qPCR analysis and GUS activity detection indicated that the expression of lysR7 was induced in the presence of galactose. The mutant Delta lysR7 caused weaker wilt disease on either Solanum lycopersicum or Capsicum annuum plants compared to both wild type GMI1000 and C Delta lysR7. Transcriptome analysis revealed that 12 upregulated and 8 downregulated differentially expressed genes (DEGs) in Delta lysR7 were restored in C Delta lysR7 relative to wild type. In particular, the expression of hrpG, a key gene responsible for type III secretion system, was downregulated. KEGG analysis revealed that, except for lysR7 gene, the 19 DEGs were most enriched in microbial metabolism in diverse environments and metabolic pathways. Conclusions The data indicate that LysR7 regulates multiple processes in association with motility, galactose metabolism and virulence in R. solanacearum. The study offers valuable evidence to understand comprehensive regulatory mechanisms mediated by LTTR family members in R. solanacearum.
Keyword ：
Galactose utilization Galactose utilization LTTRs LTTRs Motility Motility Ralstonia solanacearum Ralstonia solanacearum Transcriptome Transcriptome Virulence Virulence
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| GB/T 7714 | Saleem, Tahira , Zou, Huasong , Zhuo, Tao et al. A LysR-type regulator influencing swimming motility, galactose utilization, and virulence in Ralstonia solanacearum GMI1000 [J]. | CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE , 2024 , 11 (1) . |
| MLA | Saleem, Tahira et al. "A LysR-type regulator influencing swimming motility, galactose utilization, and virulence in Ralstonia solanacearum GMI1000" . | CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE 11 . 1 (2024) . |
| APA | Saleem, Tahira , Zou, Huasong , Zhuo, Tao , Fan, Xiaojing . A LysR-type regulator influencing swimming motility, galactose utilization, and virulence in Ralstonia solanacearum GMI1000 . | CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE , 2024 , 11 (1) . |
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Ralstonia solanacearum (Rso) causes destructive bacterial wilt across a broad range of host plants by delivering a repertoire of type III effectors. In the present study, we determined that the deletion of the type III effector RipAF1 resulted in increased virulence on Nicotiana benthamiana, Solanum lycopersicum, and Capsicum annuum plants. RipAF1 showed ADP-ribosylation activity in vivo and in vitro. Transient overexpression of RipAF1 suppressed jasmonic acid (JA) signaling and induced salicylic acid (SA) signaling. The ADP-ribosylation activity of RipAF1 was essential for JA and SA signaling mediation. Host fibrillin FBN1 was identified as a RipAF1-interactor that is ADP-ribosylated by RipAF1 directly. Most importantly, the ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane and leads to the suppression of JA signaling and induction of SA signaling. We concluded that RipAF1 mediates antagonistic crosstalk between JA and SA signaling pathways by ADP-ribosylation of FBN1.
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| GB/T 7714 | Wu, Wei , Zou, Huasong , Zheng, Huiying et al. Ralstonia solanacearum type III effector RipAF1 mediates plant resistance signaling by ADP-ribosylation of host FBN1 [J]. | HORTICULTURE RESEARCH , 2024 , 11 (8) . |
| MLA | Wu, Wei et al. "Ralstonia solanacearum type III effector RipAF1 mediates plant resistance signaling by ADP-ribosylation of host FBN1" . | HORTICULTURE RESEARCH 11 . 8 (2024) . |
| APA | Wu, Wei , Zou, Huasong , Zheng, Huiying , Chen, Xinyu , Luo, Xuming , Fan, Xiaojing et al. Ralstonia solanacearum type III effector RipAF1 mediates plant resistance signaling by ADP-ribosylation of host FBN1 . | HORTICULTURE RESEARCH , 2024 , 11 (8) . |
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摩氏假单胞菌Pseudomonas mosselii Pt A1是对多株青枯菌均有良好抑制作用的生防菌，基因组中含有完整的环脂肽合成基因簇xtlABC。为了探明摩氏假单胞菌PtA1中xtl ABC基因簇的功能，本研究利用同源重组技术获得缺失突变体ΔxtlA、ΔxtlBC和Δxtl ABC，通过Southern blot验证突变体正确后，分析其对菌株生长速率、游动性、生物膜及生防相关性状等表型的影响。研究结果显示，与野生型菌株Pt A1相比，突变体ΔxtlA、ΔxtlBC和ΔxtlABC在LB培养基中的生长速度变慢，在游动培养基上的迁移直径分别减少了12.6、12.5和13.5mm，生物膜形成量分别降低了30.41%、29.16%和30.70%，对4种青枯菌的抑菌圈直径显著减小。盆栽试验表明，接种青枯菌GMI1000后4d，处理组PtA1+GMI1000、ΔxtlA+GMI1000、ΔxtlBC+GMI1000和ΔxtlABC+GMI1000对番茄青枯病的防效分别为44.93%、9.49%、10.46%和7.66%，第10 d的防效分别为42.43%、19.56%、31.40%和24.79%，处理组PtA1+GMI1000防治效果最好，与xtlABC突变体处理组差异显著。综合分析表明xtlABC基因簇影响菌株自身的生长及生防相关性状，该基因及其合成的次生代谢产物在抑制青枯菌中起重要作用。
Keyword ：
基因簇xtlABC 基因簇xtlABC 抑菌作用 抑菌作用 摩氏假单胞菌 摩氏假单胞菌 环脂肽 环脂肽 青枯菌 青枯菌
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| GB/T 7714 | 郑汉维 , 谢文松 , 牛世霖 et al. 摩氏假单胞菌环脂肽合成基因簇xtlABC的功能研究 [J]. | 中国生物防治学报 , 2024 , 40 (03) : 620-629 . |
| MLA | 郑汉维 et al. "摩氏假单胞菌环脂肽合成基因簇xtlABC的功能研究" . | 中国生物防治学报 40 . 03 (2024) : 620-629 . |
| APA | 郑汉维 , 谢文松 , 牛世霖 , 张旭 , 卓涛 , 邱思鑫 et al. 摩氏假单胞菌环脂肽合成基因簇xtlABC的功能研究 . | 中国生物防治学报 , 2024 , 40 (03) , 620-629 . |
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Ralstonia solanacearum(Rso)causes destructive bacterial wilt across a broad range of host plants by delivering a repertoire of type Ⅲ effectors.In the present study,we determined that the deletion of the type Ⅲ effector RipAF1 resulted in increased virulence on Nicotiana benthamiana,Solanum lycopersicum,and Capsicum annuum plants.RipAF1 showed ADP-ribosylation activity in vivo and in vitro.Transient overexpression of RipAF1 suppressed jasmonic acid(JA)signaling and induced salicylic acid(SA)signaling.The ADP-ribosylation activity of RipAF1 was essential for JA and SA signaling mediation.Host fibrillin FBN1 was identified as a RipAF1-interactor that is ADP-ribosylated by RipAF1 directly.Most importantly,the ADP-ribosylation of conserved residues of FBN1 contributes to its localization to the plasma membrane and leads to the suppression of JA signaling and induction of SA signaling.We concluded that RipAF1 mediates antagonistic crosstalk between JA and SA signaling pathways by ADP-ribosylation of FBN1.
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| GB/T 7714 | Wei Wu , Huasong Zou , Huiying Zheng et al. Ralstonia solanacearum type Ⅲ effector RipAF1 mediates plant resistance signaling by ADP-ribosylation of host FBN1 [J]. | 园艺研究（英文） , 2024 , 11 (8) : 65-79 . |
| MLA | Wei Wu et al. "Ralstonia solanacearum type Ⅲ effector RipAF1 mediates plant resistance signaling by ADP-ribosylation of host FBN1" . | 园艺研究（英文） 11 . 8 (2024) : 65-79 . |
| APA | Wei Wu , Huasong Zou , Huiying Zheng , Xinyu Chen , Xuming Luo , Xiaojing Fan et al. Ralstonia solanacearum type Ⅲ effector RipAF1 mediates plant resistance signaling by ADP-ribosylation of host FBN1 . | 园艺研究（英文） , 2024 , 11 (8) , 65-79 . |
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Identifying the causative agent of a disease provides key information for diagnosis and management. Here, we identified the causative agent of a leaf spot disease prevalent in water spinach (Ipomoea aquatica) in Fujian Province, China. Ninety bacterial isolates were recovered from symptomatic leaves of water spinach collected across six growing areas in Fujian Province. Completion of Koch's postulates confirmed that 60 yellow mucoid isolates were the causative agents of the disease. PCR amplification of 16S ribosomal RNA gene sequences demonstrated that the isolates belonged to the genus Xanthomonas. We then PCR-amplified and sequenced the recA, dnaK, gyrB, and rpoD genes of the isolated pathogens for multilocus sequence analysis. In the resulting phylogenetic tree, the isolated pathogens grouped with X. euvesicatoria pv. perforans strain TC2-1. Biological and chemical tests performed on 60 isolates showed that all of them were positive in hydrogen sulfide, litmus milk, starch hydrolysis and bile-esculin tests, but negative in nitrate reduction, pectinase activity, and oxidase activity tests. The isolates could also cause disease symptoms on tomato (Solanum lycopersicum) leaves and stems but not on pepper (Capsicum annuum) plants. Based on DNA characteristics, physiological and chemical properties, and pathogenicity test results, we propose that the leaf spot disease in water spinach is caused by X. euvesicatoria pv. perforans.
Keyword ：
16S rDNA identification 16S rDNA identification Leaf spot disease Leaf spot disease Multilocus sequence analysis Multilocus sequence analysis Water spinach Water spinach Xanthomonad Xanthomonad
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| GB/T 7714 | Fan, Xiaojing , Zheng, Hanwei , Luo, Haiyan et al. Xanthomonas euvesicatoria pv. perforans is the causative agent of bacterial leaf spot on Ipomoea aquatica from Fujian Province in China [J]. | AUSTRALASIAN PLANT PATHOLOGY , 2023 , 52 (4) : 327-337 . |
| MLA | Fan, Xiaojing et al. "Xanthomonas euvesicatoria pv. perforans is the causative agent of bacterial leaf spot on Ipomoea aquatica from Fujian Province in China" . | AUSTRALASIAN PLANT PATHOLOGY 52 . 4 (2023) : 327-337 . |
| APA | Fan, Xiaojing , Zheng, Hanwei , Luo, Haiyan , Zhuo, Tao , Chen, Yong . Xanthomonas euvesicatoria pv. perforans is the causative agent of bacterial leaf spot on Ipomoea aquatica from Fujian Province in China . | AUSTRALASIAN PLANT PATHOLOGY , 2023 , 52 (4) , 327-337 . |
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Simple Summary Tobacco bacterial wilt caused by Ralstonia solanacearum brings large economic losses every year. Currently, an increasing number of biocontrol agents have been widely used in the control of this disease, but they cannot replace chemical agents, mostly due to the poorer control effect. Therefore, in this study, the avirulence gene ripAA from Ralstonia solanacearum, which determines incompatible interactions with tobacco plants, was introduced into the biocontrol agent Pseudomonas mosselii to increase the efficacy against Ralstonia solanacearum. The newly engineered strain can improve the systemic resistance and elicit a primary immune response of plants. Our research not only provides a new strategy for the genetic modification of biocontrol agents, in which a number of avirulence genes from the pathogen or plant can be tested to be expressed in different biocontrol agents to antagonize this plant disease, but also helps the study of the interaction between the phytopathogenic avirulence gene and the host. The environmental bacterium Pseudomonas mosselii produces antagonistic secondary metabolites with inhibitory effects on multiple plant pathogens, including Ralstonia solanacearum, the causal agent of bacterial wilt. In this study, an engineered P. mosselii strain was generated to express R. solanacearum ripAA, which determines the incompatible interactions with tobacco plants. The ripAA gene, together with its native promoter, was integrated into the P. mosselii chromosome. The resulting strain showed no difference in antimicrobial activity against R. solanacearum. Promoter-LacZ fusion and RT-PCR experiments demonstrated that the ripAA gene was transcribed in culture media. Compared with that of the wild type, the engineered strain reduced the disease index by 9.1% for bacterial wilt on tobacco plants. A transcriptome analysis was performed to identify differentially expressed genes in tobacco plants, and the results revealed that ethylene- and jasmonate-dependent defense signaling pathways were induced. These data demonstrates that the engineered P. mosselii expressing ripAA can improve biological control against tobacco bacterial wilt by the activation of host defense responses.
Keyword ：
bacterial wilt bacterial wilt control efficacy control efficacy defense signaling pathway defense signaling pathway Pseudomonas mosselii Pseudomonas mosselii ripAA ripAA
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| GB/T 7714 | Zhuo, Tao , Chen, Shiting , Wang, Dandan et al. Expression of the ripAA Gene in the Soilborne Pseudomonas mosselii Can Promote the Control Efficacy against Tobacco Bacterial Wilt [J]. | BIOLOGY-BASEL , 2022 , 11 (8) . |
| MLA | Zhuo, Tao et al. "Expression of the ripAA Gene in the Soilborne Pseudomonas mosselii Can Promote the Control Efficacy against Tobacco Bacterial Wilt" . | BIOLOGY-BASEL 11 . 8 (2022) . |
| APA | Zhuo, Tao , Chen, Shiting , Wang, Dandan , Fan, Xiaojing , Zhang, Xiaofeng , Zou, Huasong . Expression of the ripAA Gene in the Soilborne Pseudomonas mosselii Can Promote the Control Efficacy against Tobacco Bacterial Wilt . | BIOLOGY-BASEL , 2022 , 11 (8) . |
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通过体内外生长曲线和表型观察试验，分析不同浓度β-蒎烯对柑橘溃疡病菌(Xcc 29-1)的生长抑制作用和柑橘溃疡病症状形成的影响。利用β-葡萄糖苷酸酶基因的定性检测和实时荧光定量PCR分析不同浓度β-蒎烯对Xcc 29-1Ⅲ型分泌系统相关基因hrp G的诱导作用。结果表明:随着浓度的升高，β-蒎烯对Xcc 29-1的生长抑制增强，0.2μg·mL~(-1)的β-蒎烯能显著抑制溃疡病菌的生长繁殖，培养基中添加1μg·mL~(-1)β-蒎烯时，Xcc 29-1几乎不能生长。同时，0.1μg·mL~(-1)的β-蒎烯能有效在体内外诱导Ⅲ型分泌系统相关基因hrp G、hrp X、hrc V和hrp D6的转录表达。β-蒎烯作为柑橘的次级代谢产物可抑制Xcc 29-1的生长繁殖，抑菌作用的大小与其浓度正相关。与此同时，β-蒎烯能诱导Ⅲ型分泌系统相关基因的表达。说明β-蒎烯抑制的不是菌物的致病力，而是菌物的繁殖。
Keyword ：
hrp基因 hrp基因 β-蒎烯 β-蒎烯 抑制生长 抑制生长 柑橘溃疡病 柑橘溃疡病 诱导表达 诱导表达
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| GB/T 7714 | 叶子怡 , 范晓静 , 卓涛 et al. 柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探 [J]. | 武夷科学 , 2022 , 38 (01) : 25-30 . |
| MLA | 叶子怡 et al. "柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探" . | 武夷科学 38 . 01 (2022) : 25-30 . |
| APA | 叶子怡 , 范晓静 , 卓涛 , 邹华松 , 户勋 . 柑橘次级代谢产物β-蒎烯对柑橘溃疡病菌的生长抑制作用与机理初探 . | 武夷科学 , 2022 , 38 (01) , 25-30 . |
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青枯菌通过Ⅲ型分泌系统向寄主植物细胞分泌100多种效应蛋白，对寄主植物的抗感病性产生影响。青枯菌效应蛋白RipQ启动子区存在典型的HrpB识别序列PIP box (5′-TTCGG-N15-TTCGC-3′),但其功能尚未明确。本研究分析了RipQ在青枯菌4种演化型菌株中的分布情况。以青枯菌GMI1000为出发菌，构建ripQ缺失突变体和过表达菌株，研究效应蛋白RipQ在青枯菌-番茄植物互作中的功能。结果显示，ripQ广泛分布于除演化型IV的不同青枯菌类群中。与野生型菌株相比，ripQ突变体在番茄上的致病力有一定程度的增强，而ripQ过表达菌株的致病力显著降低。突变体和过表达菌株在培养基中的生长与野生型没有区别，但过表达菌株在番茄体内的繁殖能力下降。RipQ过表达菌株侵染番茄后hrpB、hrpG和epsA基因表达量显著下调，且能够诱导番茄叶片H_2O_2的大量累积，过敏性坏死反应标志基因hin1和水杨酸信号通路标志基因PR1a的诱导表达。另外，在番茄上瞬时表达ripQ也可以观察到H_2O_2积累及叶片细胞坏死，伴随着hin1和PR1a的上调表达。这些结果表明效应蛋白RipQ具有诱导番茄抗性的作用，从而影响青枯菌在番茄植物上的致病力。
Keyword ：
抗性 抗性 效应蛋白RipQ 效应蛋白RipQ 致病力 致病力 青枯菌 青枯菌
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| GB/T 7714 | 贵彩英 , TAHIRA Saleem , 谢文松 et al. 青枯菌GMI1000效应蛋白RipQ对致病力的作用研究 [J]. | 植物病理学报 , 2022 , 52 (05) : 801-812 . |
| MLA | 贵彩英 et al. "青枯菌GMI1000效应蛋白RipQ对致病力的作用研究" . | 植物病理学报 52 . 05 (2022) : 801-812 . |
| APA | 贵彩英 , TAHIRA Saleem , 谢文松 , 卓涛 , 户勋 , 邹华松 et al. 青枯菌GMI1000效应蛋白RipQ对致病力的作用研究 . | 植物病理学报 , 2022 , 52 (05) , 801-812 . |
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青枯模式菌株GMI1000分泌的Ⅲ型效应子RipI使致病力降低,靶定寄主植物转录因子bHLH诱导JA/ET抗病信号通路。通过预测发现RipI含有两个特殊的保守结构域,分别为整合酶结构域(58～174)和DNA聚合酶Ⅲ亚基γ和τ结构域(175～378)。酵母双杂交和荧光素酶互补实验结果显示,整合酶结构域与bHLH没有互作,但对RipⅠ与bHLH的互作有影响;DNA聚合酶Ⅲ结构域与RipⅠ存在互作关系。在本氏烟上瞬时表达RipI各个结构域发现,整合酶结构域和DNA聚合酶Ⅲ结构域单独都不能诱导细胞坏死,RipⅠ对细胞坏死的诱导需要这两个功能域共同存在。本文基于蛋白结构预测分析,研究了RipⅠ与bHLH互作及诱导植物细胞坏死的关键功能域,为认识效应子RipⅠ诱导植物抗性的分子机制提供了线索。
Keyword ：
效应蛋白RipⅠ 效应蛋白RipⅠ 转录因子bHLH93 转录因子bHLH93 青枯菌 青枯菌
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| GB/T 7714 | 赵同心 , 范晓静 , 邹华松 et al. 青枯菌Ⅲ型效应子RipI与寄主bHLH互作的关键结构域定位 [C] //植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . 2021 . |
| MLA | 赵同心 et al. "青枯菌Ⅲ型效应子RipI与寄主bHLH互作的关键结构域定位" 植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . (2021) . |
| APA | 赵同心 , 范晓静 , 邹华松 , 卓涛 . 青枯菌Ⅲ型效应子RipI与寄主bHLH互作的关键结构域定位 植物病理科技创新与绿色防控——中国植物病理学会2021年学术年会论文集 . (2021) . |
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