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学者姓名：陈湜
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Abstract ：
果蝇锤角细蜂是一种重要的斑翅果蝇蛹期寄生蜂，寄生斑翅果蝇后在寄主蛹内完成发育，进而实现对斑翅果蝇的防治。低温作为一种实用性较强的天敌贮藏处理方法，在延长天敌货架期方面发挥重要作用。以羽化率和性比为衡量指标，本文对变温贮藏和低温贮藏前的快速冷驯化对果蝇锤角细蜂生长发育的影响进行研究。变温试验结果表明，4龄幼虫期经变温T5处理贮藏10 d(72.62%)和15 d(65.99%)后果蝇锤角细蜂的羽化率与对照（76.64%）无显著差异。相同温度下，4龄幼虫期果蝇锤角细蜂经各处理后的羽化率随着贮藏时间的延长而下降。相同贮藏时间下，4龄幼虫期果蝇锤角细蜂经变温处理的羽化率显著高于同温度的持续恒温处理。同一变温处理经不同时间贮藏后羽化成蜂的性比间均不存在差异。冷驯化试验结果表明，4龄幼虫期和1龄蛹期果蝇锤角细蜂经冷驯化后的羽化率与对照无显著差异。在贮藏10 d和15 d时，4龄幼虫期果蝇锤角细蜂经冷驯化后羽化成蜂的性比显著高于直接进行低温贮藏的结果与对照。相同贮藏温度下，4龄幼虫期和1龄蛹期果蝇锤角细蜂经冷驯化后羽化成蜂的性比不随贮藏时间的延长而显著变化。研究结果可为果蝇锤角细蜂的低温变温贮藏和低温贮藏前的快速冷驯化提供参考，为天敌昆虫货架期的延长提供重要依据。
Keyword ：
低温贮藏 低温贮藏 冷驯化 冷驯化 变温 变温 斑翅果蝇 斑翅果蝇 果蝇锤角细蜂 果蝇锤角细蜂
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| GB/T 7714 | 张沁园 , 刘旭祥 , 陈俊 et al. 变温和冷驯化对果蝇锤角细蜂生长发育的影响 [J]. | 中国生物防治学报 , 2025 , 41 (02) : 251-259 . |
| MLA | 张沁园 et al. "变温和冷驯化对果蝇锤角细蜂生长发育的影响" . | 中国生物防治学报 41 . 02 (2025) : 251-259 . |
| APA | 张沁园 , 刘旭祥 , 陈俊 , 吴雨虹 , 许炜杰 , 凡庆文 et al. 变温和冷驯化对果蝇锤角细蜂生长发育的影响 . | 中国生物防治学报 , 2025 , 41 (02) , 251-259 . |
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Leluthia minqingensis sp. nov., a koinobiont endoparasitoid of Phloeosinus sinensis Schedle (Coleoptera: Scolytidae), is described from Fujian (Southeast China) . Morphological characteristics and photographs differentiating it from similar species are provided.
Keyword ：
endoparasitoid endoparasitoid natural enemy natural enemy Palaearctic Palaearctic taxonomy taxonomy
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| GB/T 7714 | Zhang, Ke-Ran , Geng, Jun-Ling , Chen, Shi et al. Revisional notes on the genus Leluthia Cameron (Hymenoptera: Braconidae: Doryctinae) from China, with description of a new species parasitizing Phloeosinus sinensis Schedle (Coleoptera: Scolytidae) [J]. | ZOOTAXA , 2025 , 5618 (4) : 595-600 . |
| MLA | Zhang, Ke-Ran et al. "Revisional notes on the genus Leluthia Cameron (Hymenoptera: Braconidae: Doryctinae) from China, with description of a new species parasitizing Phloeosinus sinensis Schedle (Coleoptera: Scolytidae)" . | ZOOTAXA 5618 . 4 (2025) : 595-600 . |
| APA | Zhang, Ke-Ran , Geng, Jun-Ling , Chen, Shi , Liang, Guang-Hong , Zheng, Min-Lin . Revisional notes on the genus Leluthia Cameron (Hymenoptera: Braconidae: Doryctinae) from China, with description of a new species parasitizing Phloeosinus sinensis Schedle (Coleoptera: Scolytidae) . | ZOOTAXA , 2025 , 5618 (4) , 595-600 . |
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Drosophila suzukii (Diptera: Drosophilidae) is a polyphagous pest native to Asia that can cause serious economic damage. In this study, the effects of low-temperature storage on growth and development of Trichopria drosophilae were studied by using the methods of cold storage pupae to evaluate the cold storage tolerance and quality assessment of T. drosophilae, providing a theoretical basis for solving the mass production, storage and concentrated release of T. drosophilae. The results showed that the emergence rate of the 4th instar larvae decreased with extension of storage time from 10 to 20 d at 8 degrees C, 10 degrees C and 12 degrees C. The emergence rate remained as high as 79.24% after storage for 15 d at 16 degrees C. At 14 degrees C and 16 degrees C, the emergence rate of the 1-day-old pupae was not significantly different from that of control (77.27%). The above results indicated that the 4th instar and 1-day-old pupae of T. drosophilae were more suitable for long-term storage at 14 degrees C and 16 degrees C. Under the above experimental conditions, T. drosophila quality after cold storage for 10, 15 and 20 d was continuously investigated. The results showed that parasitism rates of different stages after treatment were significantly affected by storage temperature and time. Long-term cold storage could reduce the emergence rate and sex ratio of F1 generation. The emergence time of the 1-day-old pupae was gradually shortened with extension of storage time at 16 degrees C. The starvation-resistant lifespan of T. drosophilae after cold storage was significantly longer than that of control. This study results will further clarify the effects of cold temperature storage on growth and development of T. drosophilae.
Keyword ：
Biological control Biological control Low-temperature storage Low-temperature storage Natural enemy Natural enemy Shelf life Shelf life Spotted wing drosophila Spotted wing drosophila
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| GB/T 7714 | Zhang, Qinyuan , Liu, Xuxiang , Wu, Yuhao et al. Effects of cold storage on the growth and development of Trichopria drosophilae (Hymenoptera: Diapriidae) [J]. | SCIENTIFIC REPORTS , 2025 , 15 (1) . |
| MLA | Zhang, Qinyuan et al. "Effects of cold storage on the growth and development of Trichopria drosophilae (Hymenoptera: Diapriidae)" . | SCIENTIFIC REPORTS 15 . 1 (2025) . |
| APA | Zhang, Qinyuan , Liu, Xuxiang , Wu, Yuhao , Zhang, Run , Yang, Yongbang , Fan, Qingwen et al. Effects of cold storage on the growth and development of Trichopria drosophilae (Hymenoptera: Diapriidae) . | SCIENTIFIC REPORTS , 2025 , 15 (1) . |
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本文分别利用3种紫外线(UVA、UVB、UVC)灯辐照黑腹果蝇蛹,研究辐照对黑腹果蝇的羽化率、性比、飞出率、死蛹率、成虫干重、蛹重及F1羽化率、性比、死蛹率、成虫干重的影响。结果表明:辐照后黑腹果蝇羽化率均显著降低,其中UVB辐照6h后的黑腹果蝇蛹羽化率最低,为10.00%;经过UVA辐照9h组的性比平均值为2.32,显著高于其他处理组;经过UVB辐照后,黑腹果蝇飞出率显著降低,其中UVB处理6h后黑腹果蝇飞出率低至4.00%;经过UVB辐照的死蛹率显著高于对照组和其他组,其中辐照6 h组黑腹果蝇蛹的死亡率平均值达到89.33%,最高达94.00%。经过紫外线辐照后各组蛹重与对照组相比都有不同程度的增加,且差异显著;经过UVB、UVC辐照后羽化的黑腹果蝇成虫干重显著低于对照组;经过紫外线辐照后黑腹果蝇F1成虫干重相比对照组均有所降低,与对照组差异显著。本研究可为昆虫的紫外线诱变和害虫生物防治提供参考。
Keyword ：
紫外线 紫外线 辐照 辐照 黑腹果蝇 黑腹果蝇
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| GB/T 7714 | 刘旭祥 , 仪传冬 , 敖国富 et al. 三种紫外线辐照对黑腹果蝇蛹的影响 [C] //中国核学会2023年学术年会 . 2023 . |
| MLA | 刘旭祥 et al. "三种紫外线辐照对黑腹果蝇蛹的影响" 中国核学会2023年学术年会 . (2023) . |
| APA | 刘旭祥 , 仪传冬 , 敖国富 , 陈湜 , 季清娥 . 三种紫外线辐照对黑腹果蝇蛹的影响 中国核学会2023年学术年会 . (2023) . |
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Diachasmimorpha anshunensis sp. nov., a koinobiont endoparasitoid of larvae of Zeugodacus tau (Walker) (Tephritidae: Diptera), is discovered from Guizhou (Southwest China) and is described by multiple forms of evidence. Morphological characteristics, photographs, and molecular data differentiating it from similar species are provided. Several biological characteristics of this new parasitoid, observed in a laboratory setting, are also provided as evidence to separate it from the most similar species in appearance.
Keyword ：
endoparasitoid endoparasitoid Palaearctic Palaearctic taxonomy taxonomy Zeugodacus Zeugodacus
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| GB/T 7714 | Ao, Guo-Fu , Shi, Shuang , Zheng, Min-Lin et al. A new species of Diachasmimorpha Viereck (Hymenoptera: Braconidae: Opiinae) from China [J]. | ZOOTAXA , 2022 , 5116 (3) : 429-438 . |
| MLA | Ao, Guo-Fu et al. "A new species of Diachasmimorpha Viereck (Hymenoptera: Braconidae: Opiinae) from China" . | ZOOTAXA 5116 . 3 (2022) : 429-438 . |
| APA | Ao, Guo-Fu , Shi, Shuang , Zheng, Min-Lin , Chen, Shi , Ji, Qing-E . A new species of Diachasmimorpha Viereck (Hymenoptera: Braconidae: Opiinae) from China . | ZOOTAXA , 2022 , 5116 (3) , 429-438 . |
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One environmentally friendly method used to manage Bactrocera dorsalis (Hendel), a key agricultural pest of substantial economic importance, is the sterile insect technique (SIT). Nevertheless, several deficiencies related to this strategy impair the success of the SIT, including the inferior performance of released sterile males compared with wild males, which could be partly solved by the utilization of gut symbionts as probiotic dietary components. In this study, a culture-dependent method was used to isolate and characterize gut-associated bacterial species in adult B. dorsalis genetic sexing strain (GSS) males. In addition, three bacterial isolates from the Enterobacteriaceae family, namely, Enterobacter sp., Morganella morganii, and Moellerella wisconsensis, were used as supplements in larval and adult diets to assess their effects on the life-history traits of irradiated males. Consistent with many previous studies, Enterobacter spp. was shown to be beneficial, with some quality control indices, such as adult size, pupal weight, survival rate under stress and nutritionally rich conditions, and mating competitiveness, being significantly increased, while slight nonsignificant increases in emergence rate and flight ability were observed. Conversely, the M. morganii and M. wisconsensis strains both had negative effects on irradiated male fitness and mating competitiveness. Our results, in combination with those of earlier studies, can contribute to improving the effectiveness of SIT application by enhancing the different aspects of augmentative rearing and biological traits of pests under laboratory rearing conditions.
Keyword ：
bacteria-enriched diet bacteria-enriched diet GSS GSS irradiation irradiation oriental fruit fly oriental fruit fly SIT SIT
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| GB/T 7714 | Zhang, Qiwen , Cai, Pumo , Wang, Bo et al. Manipulation of Gut Symbionts for Improving the Sterile Insect Technique: Quality Parameters of Bactrocera dorsalis (Diptera: Tephritidae) Genetic Sexing Strain Males After Feeding on Bacteria-Enriched Diets [J]. | JOURNAL OF ECONOMIC ENTOMOLOGY , 2021 , 114 (2) : 560-570 . |
| MLA | Zhang, Qiwen et al. "Manipulation of Gut Symbionts for Improving the Sterile Insect Technique: Quality Parameters of Bactrocera dorsalis (Diptera: Tephritidae) Genetic Sexing Strain Males After Feeding on Bacteria-Enriched Diets" . | JOURNAL OF ECONOMIC ENTOMOLOGY 114 . 2 (2021) : 560-570 . |
| APA | Zhang, Qiwen , Cai, Pumo , Wang, Bo , Liu, Xuxiang , Lin, Jia , Hua, Ruohan et al. Manipulation of Gut Symbionts for Improving the Sterile Insect Technique: Quality Parameters of Bactrocera dorsalis (Diptera: Tephritidae) Genetic Sexing Strain Males After Feeding on Bacteria-Enriched Diets . | JOURNAL OF ECONOMIC ENTOMOLOGY , 2021 , 114 (2) , 560-570 . |
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The sterile insect technique (SIT) has been developed as a component of area-wide integrated pest management approaches to control the populations of Aedes albopictus, a mosquito vector capable of transmission of dengue, Zika and chikungunya viruses. One of the key factors for the success of SIT is the requirement of high biological quality sterile males, which upon their release would be able to compete with wild males for matings with wild females in the field. In insects, gut bacteriome have played a catalytic role during evolution significantly affecting several aspects of their biology and ecology. Given the importance of gut-associated bacterial species for the overall ecological fitness and biological quality of their hosts, it is of interest to understand the effects of radiation on the gut-associated bacteriome of Ae. albopictus. In this study, the effect of radiation on the composition and density levels of the gut-associated bacterial species at the pupal stage as well as at 1- and 4-day-old males and females was studied using 16S rRNA gene-based next generation sequencing (NGS) and quantitative PCR (qPCR) approaches. Age, diet, sex, and radiation were shown to affect the gut-associated bacterial communities, with age having the highest impact triggering significant changes on bacterial diversity and clustering among pupae, 1- and 4-day-old adult samples. qPCR analysis revealed that the relative density levels of Aeromonas are higher in male samples compared to all other samples and that the irradiation triggers an increase in the density levels of both Aeromonas and Elizabethkingia in the mosquito gut at specific stages. Our results suggest that Aeromonas could potentially be used as probiotics to enhance protandry and sex separation in support of SIT applications against Ae. albopictus, while the functional role of Elizabethkingia in respect to oxidative stress and damage in irradiated mosquitoes needs further investigation.
Keyword ：
16S rRNA gene 16S rRNA gene Aedes albopictus Aedes albopictus Aeromonas Aeromonas Elizabethkingia Elizabethkingia gut bacteriome gut bacteriome
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| GB/T 7714 | Zhang, Dongjing , Chen, Shi , Abd-Alla, Adly M. M. et al. The Effect of Radiation on the Gut Bacteriome of Aedes albopictus [J]. | FRONTIERS IN MICROBIOLOGY , 2021 , 12 . |
| MLA | Zhang, Dongjing et al. "The Effect of Radiation on the Gut Bacteriome of Aedes albopictus" . | FRONTIERS IN MICROBIOLOGY 12 (2021) . |
| APA | Zhang, Dongjing , Chen, Shi , Abd-Alla, Adly M. M. , Bourtzis, Kostas . The Effect of Radiation on the Gut Bacteriome of Aedes albopictus . | FRONTIERS IN MICROBIOLOGY , 2021 , 12 . |
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分别利用三种紫外灯(UVA、UVB、UVC)辐照黑腹果蝇蛹,研究辐照对黑腹果蝇的羽化率、性比、飞出率、死蛹率、成虫干重、蛹重以及F1羽化率、F1性比、F1死蛹率、F1成虫干重的影响。结果表明:辐照后黑腹果蝇羽化率均显著降低,其中UVB辐照6 h后的黑腹果蝇蛹羽化率最低,为10.00%;经过UVA辐照9 h组的性比平均值为2.32,显著高于其他处理组;经过UVB辐照后,黑腹果蝇飞出率显著降低,其中UVB处理6 h后黑腹果蝇飞出率低至4.00%;经过UVB辐照的死蛹率显著高于对照组和其他组,其中辐照6h组黑腹果蝇蛹的死亡率平均值达到89.33%,最高达94.00%。经过紫外线辐照后各组蛹重与对照组相比都有不同程度的增加,且差异显著;经过UVB、UVC辐照后羽化的黑腹果蝇成虫干重显著低于对照组;经过紫外线辐照后黑腹果蝇F1成虫干重相比对照组均有所降低,与对照组差异显著。本研究可为昆虫的紫外线诱变和害虫生物防治提供参考。
Keyword ：
紫外线 紫外线 辐照 辐照 黑腹果蝇 黑腹果蝇
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| GB/T 7714 | 刘旭祥 , 仪传冬 , 敖国富 et al. 三种紫外线辐照对黑腹果蝇蛹的影响 [J]. | 中国生物防治学报 , 2021 , 37 (02) : 228-234 . |
| MLA | 刘旭祥 et al. "三种紫外线辐照对黑腹果蝇蛹的影响" . | 中国生物防治学报 37 . 02 (2021) : 228-234 . |
| APA | 刘旭祥 , 仪传冬 , 敖国富 , 陈湜 , 季清娥 . 三种紫外线辐照对黑腹果蝇蛹的影响 . | 中国生物防治学报 , 2021 , 37 (02) , 228-234 . |
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本实用新型涉及一种收集黑腹果蝇老熟幼虫和蛹的装置，包括竖直置于纸质漏斗内的方盒，所述纸质漏斗下端延伸至抽屉柜内顶部，纸质漏斗呈锥状，方盒下端与纸质漏斗内壁接触处形成弧状的集虫缝，所述抽屉柜内设置有三层，自上而下分别为光线梯度层以及两均带有抽屉的集虫层、清理层，光线梯度层上下贯通，集虫层的集虫抽屉内底部固设有集虫槽，该集虫槽内垫设有集虫纸，该收集黑腹果蝇老熟幼虫和蛹的装置结构简单，便捷高效。
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| GB/T 7714 | 陈湜 , 刘旭祥 , 季清娥 et al. 一种收集黑腹果蝇老熟幼虫和蛹的装置 : CN202020627031.0[P]. | 2020-04-23 . |
| MLA | 陈湜 et al. "一种收集黑腹果蝇老熟幼虫和蛹的装置" : CN202020627031.0. | 2020-04-23 . |
| APA | 陈湜 , 刘旭祥 , 季清娥 , 郑敏琳 . 一种收集黑腹果蝇老熟幼虫和蛹的装置 : CN202020627031.0. | 2020-04-23 . |
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本发明涉及一种多功能诱集果蝇装置，包括盒体，所述盒体上端开口处安装有盒盖，所述盒体上部周侧的内壁及外壁上均设置有诱虫黄板，所述盒体周侧中部设置有透气窗口及若干入虫口，盒体内底部承装有诱虫液，所述透气窗口内安装有通气格栅网，盒体内设置有若干入虫导管，入虫导管与入虫口一一对应设置，入虫导管一端与入虫口相连通，另一端连通盒体内壁且位于诱虫液的液面以上，本装置结构简单，设计合理，成本低廉，拆装及使用方便，诱集高效、省时省力，效果持久。
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| GB/T 7714 | 刘旭祥 , 张沁园 , 林嘉 et al. 一种多功能诱集果蝇装置 : CN202022426042.5[P]. | 2020-10-28 . |
| MLA | 刘旭祥 et al. "一种多功能诱集果蝇装置" : CN202022426042.5. | 2020-10-28 . |
| APA | 刘旭祥 , 张沁园 , 林嘉 , 敖国富 , 李吉敏 , 陈湜 et al. 一种多功能诱集果蝇装置 : CN202022426042.5. | 2020-10-28 . |
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