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学者姓名：李猷
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Abstract ：
本发明公开了一种抗病毒肽及其防治水稻病毒病的应用。本发明打破了从土壤微生物寻找抗病毒肽的传统思路，创新性地从动物生物体内寻找可能的抗病毒肽；经一系列前期研究，最终确定了8条抗病毒肽序列，经验证，本发明提供的天然来源的抗病毒肽均对水稻病毒病有显著的防治作用，为水稻病毒病的防治提供了一种新的农药，对环境无害，效果非常明显。
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| GB/T 7714 | 魏太云 , 杜宇 , 李猷 et al. 抗病毒肽及其防治水稻病毒病的应用 : CN202510341064.6[P]. | 2025-03-21 . |
| MLA | 魏太云 et al. "抗病毒肽及其防治水稻病毒病的应用" : CN202510341064.6. | 2025-03-21 . |
| APA | 魏太云 , 杜宇 , 李猷 , 宾羽 , 陈倩 . 抗病毒肽及其防治水稻病毒病的应用 : CN202510341064.6. | 2025-03-21 . |
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本发明属于植物病害防治技术领域，具体涉及一种柑橘木虱源的抗菌肽DcAMP1的制备和应用。所述抗菌肽DcAMP1的氨基酸序列为SEQ ID NO.1，编码所述抗菌肽DcAMP1的基因的核苷酸序列为SEQ ID NO.2。本发明采用原核表达质粒构建包含编码抗菌肽DcAMP1的基因的重组质粒，实现了抗菌肽DcAMP1的原核表达，方法简单易行。原核表达获得的抗菌肽DcAMP1在纯化后对柑橘黄龙病菌近缘细菌费氏中华根瘤菌具有明显的抑菌效果，可显著降低带有柑橘黄龙病菌的甜橙树中的柑橘黄龙病菌滴度。
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| GB/T 7714 | 魏太云 , 李猷 , 宾羽 et al. 一种柑橘木虱源的抗菌肽DcAMP1的制备和应用 : CN202510702629.9[P]. | 2025-05-28 . |
| MLA | 魏太云 et al. "一种柑橘木虱源的抗菌肽DcAMP1的制备和应用" : CN202510702629.9. | 2025-05-28 . |
| APA | 魏太云 , 李猷 , 宾羽 , 陈倩 , 彭洪艳 . 一种柑橘木虱源的抗菌肽DcAMP1的制备和应用 : CN202510702629.9. | 2025-05-28 . |
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本发明属于植物病害防治技术领域，具体涉及一种柑橘木虱源的抗菌肽DcAMP2的制备和应用。所述抗菌肽DcAMP2的氨基酸序列为SEQ ID NO.1，编码所述抗菌肽DcAMP2的基因的核苷酸序列为SEQ ID NO.2。本发明采用原核表达质粒构建包含编码抗菌肽DcAMP2的基因的重组质粒，实现了抗菌肽DcAMP2的原核表达，方法简单易行。原核表达获得的抗菌肽DcAMP2在纯化后对柑橘黄龙病菌近缘细菌费氏中华根瘤菌具有明显的抑菌效果，可显著降低带有柑橘黄龙病菌的甜橙树中的柑橘黄龙病菌滴度。
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| GB/T 7714 | 魏太云 , 李猷 , 陈倩 et al. 一种柑橘木虱源的抗菌肽DcAMP2的制备和应用 : CN202510699670.5[P]. | 2025-05-28 . |
| MLA | 魏太云 et al. "一种柑橘木虱源的抗菌肽DcAMP2的制备和应用" : CN202510699670.5. | 2025-05-28 . |
| APA | 魏太云 , 李猷 , 陈倩 , 宾羽 , 杜宇 . 一种柑橘木虱源的抗菌肽DcAMP2的制备和应用 : CN202510699670.5. | 2025-05-28 . |
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本发明公开了一种柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用。本发明通过DcMucin‑like多克隆抗体的制备，首次验证了DcMucin‑like是柑橘木虱的唾液蛋白，并随木虱取食释放到植株组织中参与唾液鞘形成。本发明弥补了木虱唾液蛋白抗体制备和研究的空白，制备的柑橘木虱唾液蛋白多克隆抗体对DcMucin重组蛋白具有良好的特异性，基于该抗体免疫荧光标记观察木虱唾液鞘，为进一步研究DcMucin在黄龙病菌‑木虱‑柑橘三者互作中的生物学功能打下基础，为进一步获得柑橘木虱绿色防控特异性靶标奠定基础。
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| GB/T 7714 | 宾羽 , 魏太云 , 蒋丽琴 et al. 柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用 : CN202411432625.5[P]. | 2024-10-14 . |
| MLA | 宾羽 et al. "柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用" : CN202411432625.5. | 2024-10-14 . |
| APA | 宾羽 , 魏太云 , 蒋丽琴 , 李猷 . 柑橘木虱唾液蛋白DcMucin的抗体及其制备方法和应用 : CN202411432625.5. | 2024-10-14 . |
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Xenophagy is an important antibacterial defense mechanism that many organisms use to engulf intracellular pathogens. However, the mechanisms of xenophagy triggered by insect-borne plant bacteria are not well understood. Candidatus Liberibacter asiaticus (CLas) causes Huanglongbing, which poses a serious threat to citrus production. CLas is a phloem-limited unculturable bacterium that is transmitted by the Asian citrus psyllid in a persistent and propagative manner in nature. Here, we found that CLas infection in the gut of psyllids triggered a mild and anti-bacterial xenophagy. Xenophagy limited excessive propagation of CLas to maintain psyllid survival, because overload of CLas was detrimental to psyllid life. Furthermore, the outer membrane beta-barrel protein (OMBB) of CLas is the key secreted protein that induces xenophagy in psyllids by interacting with ATG8 and ATG14. OMBB can independently induce autophagy in psyllid and non-host cells. Together, these results revealed that an insect-borne plant bacterium activates mild xenophagy to control its propagation, thereby achieving persistent infection in insect vectors.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus Huanglongbing Huanglongbing Persistent infection Persistent infection Psyllid Psyllid Xenophagy Xenophagy
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| GB/T 7714 | Yu, Zhongkai , Guo, Yuxin , Chen, Hongyan et al. A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 297 . |
| MLA | Yu, Zhongkai et al. "A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection" . | MICROBIOLOGICAL RESEARCH 297 (2025) . |
| APA | Yu, Zhongkai , Guo, Yuxin , Chen, Hongyan , Wan, Wenqiang , Hu, Mengting , Li, You et al. A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection . | MICROBIOLOGICAL RESEARCH , 2025 , 297 . |
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Janus kinase/signal transducer and activator of transcription (JAK/STAT) immune response traditionally requires cytokine binding to receptors for activation; however, it remains unclear whether cytokine receptors function as direct pattern recognition receptors (PRRs) that recognise viral envelopes. In this study, we demonstrate that cytokine receptor Domeless (Dome) in leafhopper Recilia dorsalis directly recognises glycoprotein (G) of an important rice rhabdovirus. This binding induces Dome dimerisation, which initiates JAK/STAT pathway and leads to STAT-dependent expression of antiviral immune effector in R. dorsalis (RdIE1). RdIE1 suppresses viral replication by inhibiting the RNA-binding activity of viral large polymerase (L). Dome residue T659 is essential for G recognition and L residues E774 and D775 are required for RdIE1 target. Notably, virus-encoded phosphoprotein competitively inhibits the phosphorylation of STAT by casein kinase II (CK2 beta), thereby abrogating STAT-dependent transactivation of target genes. Our findings redefine Dome as a viral sensor and reveal a biphasic strategy that balances antiviral immunity with persistent viral infection in insect vectors.
Keyword ：
antiviral immune effector antiviral immune effector JAK/STAT pathway JAK/STAT pathway pathogens pathogens persistent viral infection persistent viral infection rhabdovirus rhabdovirus signalling signalling
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| GB/T 7714 | Yu, Tingting , Li, You , Xu, Mengjia et al. Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 49 (1) : 94-110 . |
| MLA | Yu, Tingting et al. "Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor" . | PLANT CELL AND ENVIRONMENT 49 . 1 (2025) : 94-110 . |
| APA | Yu, Tingting , Li, You , Xu, Mengjia , Wan, Zhihao , Jia, Dongsheng , Wei, Taiyun . Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor . | PLANT CELL AND ENVIRONMENT , 2025 , 49 (1) , 94-110 . |
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【目的】黏液样蛋白（mucin-like protein）参与半翅目昆虫取食过程中唾液鞘的形成。本研究旨在制备柑橘木虱（Diaphorina citri）黏液样蛋白DcMucin-like特异性抗体并对木虱取食孔进行免疫荧光标记，为DcMucin-like生物学功能研究提供依据。【方法】通过解剖柑橘木虱唾液腺、中肠、卵巢和精巢组织，根据木虱DcMcuin-like序列设计特异性引物，利用实时荧光定量PCR检测分析各组织中DcMcuin-like在转录水平的差异；扩增无信号肽的DcMucin-like序列并插入pET-28a中构建重组质粒，测序验证后转化Rosetta表达菌株，以终浓度0.5 mmol·L-1 IPTG在37℃下振荡培养8 h诱导重组蛋白表达，SDS-PAGE凝胶电泳检测重组蛋白表达情况；扩大培养后裂解菌体，裂解液上清经Ni-NTA纯化作为抗原免疫家兔5次，纯化血清IgG获得DcMucin-like多克隆抗体，Western blot检测抗体特异性；利用实时荧光定量PCR和Western blot比较健康木虱和感染黄龙病菌（Candidatus Liberibacter asiaticus,CLas）木虱中DcMucin-like在转录水平和蛋白水平的差异；利用交联荧光素（FITC）的DcMucin-like多克隆抗体标记木虱取食后的柑橘叶片，在共聚焦显微镜下观察柑橘木虱取食孔和唾液鞘。【结果】实时荧光定量PCR检测显示DcMcuin-like在木虱唾液腺中大量表达，且表达量显著高于中肠、卵巢和精巢组织。携带有pET28a-DcMucin的Rosetta表达菌株经IPTG诱导后在菌体裂解液的上清中获得大量表达的重组蛋白。重组蛋白免疫家兔后取抗血清纯化IgG,Western blot结果表明获得特异性好的DcMucin-like多克隆抗体。实时荧光定量PCR和Western blot分析结果显示DcMucin-like在木虱响应黄龙病菌侵染时上调表达。DcMucin-like(FITC)荧光抗体标记木虱取食后的柑橘叶片组织切片，共聚焦显微镜下观察到木虱的取食孔，并在取食孔周围检测到特异的FITC荧光信号，表明DcMucin-like随木虱取食释放到植株组织中参与唾液鞘形成。【结论】DcMucin-like在柑橘木虱唾液腺中高表达并响应黄龙病菌侵染上调表达；成功制备了特异性好的柑橘木虱DcMucin-l...
Keyword ：
DcMucin-like DcMucin-like 免疫荧光 免疫荧光 唾液鞘蛋白 唾液鞘蛋白 抗体制备 抗体制备 柑橘木虱 柑橘木虱
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| GB/T 7714 | 蒋丽琴 , 苏巧灵 , 李猷 et al. 柑橘木虱黏液样蛋白DcMucin-like抗体制备及应用 [J]. | 中国农业科学 , 2025 , 58 (02) : 281-290 . |
| MLA | 蒋丽琴 et al. "柑橘木虱黏液样蛋白DcMucin-like抗体制备及应用" . | 中国农业科学 58 . 02 (2025) : 281-290 . |
| APA | 蒋丽琴 , 苏巧灵 , 李猷 , 魏太云 , 宾羽 . 柑橘木虱黏液样蛋白DcMucin-like抗体制备及应用 . | 中国农业科学 , 2025 , 58 (02) , 281-290 . |
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Huanglongbing (HLB), one of the most destructive diseases of citrus, is caused by the fastidious Gram-negative alpha-proteobacteria bacterium Candidatus Liberibacter asiaticus (CLas) and vectored by the Asian citrus psyllid (ACP), Diaphorina citri. Currently, there are no highly effective management strategies for HLB. This review focuses on specific aspects of HLB research, including the global distribution of Candidatus Liberibacter species in citrus, HLB status in major citrus-producing countries and plant-pathogen interactions. The elucidation of virulence effectors used by CLas is explored, focusing on the molecular mechanisms that enable the pathogen to manipulate the host plant, and the insect vector ACP to evade their immune systems. Biotechnological approaches and possible management methods for minimizing HLB and ACP risks are reviewed. This review provides a summary of the CLas Sec-delivered effectors, CLas flagellar genes, tight adherence pilus, transcriptional regulators and facilitates the development of integrated and sustainable solutions to safeguard citrus cultivation. (c) 2025 Society of Chemical Industry.
Keyword ：
biotechnological approaches biotechnological approaches CLas CLas Diaphorina citri Diaphorina citri host-pathogen interactions host-pathogen interactions management methods management methods virulence effectors virulence effectors
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| GB/T 7714 | Younas, Muhammad , Zheng, Zheng , Atiq, Muhammad et al. Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies [J]. | PEST MANAGEMENT SCIENCE , 2025 , 81 (10) : 6076-6094 . |
| MLA | Younas, Muhammad et al. "Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies" . | PEST MANAGEMENT SCIENCE 81 . 10 (2025) : 6076-6094 . |
| APA | Younas, Muhammad , Zheng, Zheng , Atiq, Muhammad , Wei, Taiyun , Li, You . Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies . | PEST MANAGEMENT SCIENCE , 2025 , 81 (10) , 6076-6094 . |
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A new ambrosia beetle species of Anisandrus Ferrari, 1867, namelyA. tuberculatus sp. nov., is described from Chongqing, Shaanxi and Sichuan of Western China. Descriptions and illustrations are provided based on specimens from type materials found in Yintiaoling Nature Reserve of Chongqing.
Keyword ：
new records new records species discovery species discovery taxonomy taxonomy Xyleborine ambrosia Xyleborine ambrosia
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| GB/T 7714 | Lin, Wei , Smith, Sarah M. , Xu, Miaofeng et al. A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China [J]. | ZOOTAXA , 2025 , 5652 (1) : 195-200 . |
| MLA | Lin, Wei et al. "A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China" . | ZOOTAXA 5652 . 1 (2025) : 195-200 . |
| APA | Lin, Wei , Smith, Sarah M. , Xu, Miaofeng , Li, You . A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China . | ZOOTAXA , 2025 , 5652 (1) , 195-200 . |
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Lysozymes are well-known for their ability to cleave bacterial peptidoglycan, but their potential to hydrolyze viral components as a form of antiviral defense remains poorly understood. This study demonstrates that insect i-type lysozymes (Lyz-I1), regulated by the Toll signaling pathway, function as proteases that directly cleave viral proteins. Structural and functional analyses reveal that the catalytic dyad Glu34/Asp50 in leafhopper Lyz-I1, while retaining its essential role in bacterial peptidoglycan hydrolysis, also mediates specific binding to Lys180 on the viroplasm protein Pns9 of rice gall dwarf virus (RGDV). This interaction catalyzes the cleavage of the adjacent peptide bond of Lys180, leading to Pns9 degradation, which disrupts viroplasm assembly and inhibits viral replication. Notably, this proteolytic antiviral mechanism of Lyz-I1 shows evolutionary conservation across major rice reoviruses and their respective leafhopper or planthopper vectors. Additionally, leafhopper Lyz-I1 undergoes liquid-liquid phase separation, forming biomolecular condensates that concentrate Pns9 and enhance proteolytic efficiency. Critically, exogenous application of Lyz-I1 not only effectively reduces viral titer and disease symptoms in RGDV-infected rice plants but also induces plant immune defense. Consequently, this work provides the evidence that lysozymes can function as specific antiviral proteases, establishing a foundation for innovative control strategies against viral diseases.
Keyword ：
antiviral protease antiviral protease liquid-liquid phase separation liquid-liquid phase separation lysozyme lysozyme rice viruses rice viruses Toll signaling pathway Toll signaling pathway
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| GB/T 7714 | Du, Yu , Xiao, Yuqing , Hu, Manman et al. Insect I-Type Lysozymes Function as Antiviral Proteases by Forming Biomolecular Condensates [J]. | ADVANCED SCIENCE , 2025 . |
| MLA | Du, Yu et al. "Insect I-Type Lysozymes Function as Antiviral Proteases by Forming Biomolecular Condensates" . | ADVANCED SCIENCE (2025) . |
| APA | Du, Yu , Xiao, Yuqing , Hu, Manman , Yang, Jinhua , Li, You , Wei, Taiyun . Insect I-Type Lysozymes Function as Antiviral Proteases by Forming Biomolecular Condensates . | ADVANCED SCIENCE , 2025 . |
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