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Investigating the Citrus Aphid Species in Zhejiang, China: Morphometric Analysis and Genetic Distinctions SCIE
期刊论文 | 2025 , 54 (1) | NEOTROPICAL ENTOMOLOGY
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Aphids (Hemiptera: Aphididae) are well-known agricultural pests, affecting global crop production both through direct feeding and as the vector of multiple plant viruses. The classification of citrus aphid species was outdated and limited within the Zhejiang Province, China. A comprehensive investigation was conducted to determine the aphid species associated with citrus cultivars from 2019 to 2024. The present study included an analysis of occurrence rate, ecological contexts, and microscope slides photographs of apterous viviparous female, along with the key morphometric parameters such as body length, antenna, rostrum, hind tarsus, cauda, and siphunculi. Our morphological and molecular data revealed five species in this particular citrus region. Among the identified species, Aphis (Aphis) gossypii Glover and A. (A.) spiraecola Patch were the most prevalent, followed by A. (A.) craccivora Koch, A. (Toxoptera) citricidus (Kirkaldy), and A. (T.) aurantii Boyer de Fonscolombe. Furthermore, the genetic variation among aphid clades was assessed using Kimura 2-parameter (K2P) model. The results showed that citrus aphid species exhibits low intraspecific genetic variation and high interspecific genetic variation. Although the morphometric parameters of these aphid species are relatively similar, they can be distinguished through extensive morphological characteristics and cytochrome c oxidase subunit I (COI) fragment. Additionally, this study also addresses the historical and current distinctions among A. (A.) spiraecola and A. (T.) citricidus. These findings will significantly contribute to the ongoing research and management of citrus aphids in citrus plantations.

Keyword :

DNA DNA Genetic variation Genetic variation Identification Identification Morphology Morphology Pests Pests Viruses Viruses

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GB/T 7714 Lu, Jia , Li, You , Liu, Shunmin et al. Investigating the Citrus Aphid Species in Zhejiang, China: Morphometric Analysis and Genetic Distinctions [J]. | NEOTROPICAL ENTOMOLOGY , 2025 , 54 (1) .
MLA Lu, Jia et al. "Investigating the Citrus Aphid Species in Zhejiang, China: Morphometric Analysis and Genetic Distinctions" . | NEOTROPICAL ENTOMOLOGY 54 . 1 (2025) .
APA Lu, Jia , Li, You , Liu, Shunmin , Younas, Muhammad , Lu, Lianming , Pu, Zhanxu et al. Investigating the Citrus Aphid Species in Zhejiang, China: Morphometric Analysis and Genetic Distinctions . | NEOTROPICAL ENTOMOLOGY , 2025 , 54 (1) .
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一种柑橘木虱源的抗菌肽DcAMP2的制备和应用 ipsunlight
专利 | 2025-05-28 | CN202510699670.5
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本发明属于植物病害防治技术领域,具体涉及一种柑橘木虱源的抗菌肽DcAMP2的制备和应用。所述抗菌肽DcAMP2的氨基酸序列为SEQ ID NO.1,编码所述抗菌肽DcAMP2的基因的核苷酸序列为SEQ ID NO.2。本发明采用原核表达质粒构建包含编码抗菌肽DcAMP2的基因的重组质粒,实现了抗菌肽DcAMP2的原核表达,方法简单易行。原核表达获得的抗菌肽DcAMP2在纯化后对柑橘黄龙病菌近缘细菌费氏中华根瘤菌具有明显的抑菌效果,可显著降低带有柑橘黄龙病菌的甜橙树中的柑橘黄龙病菌滴度。

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GB/T 7714 魏太云 , 李猷 , 陈倩 et al. 一种柑橘木虱源的抗菌肽DcAMP2的制备和应用 : CN202510699670.5[P]. | 2025-05-28 .
MLA 魏太云 et al. "一种柑橘木虱源的抗菌肽DcAMP2的制备和应用" : CN202510699670.5. | 2025-05-28 .
APA 魏太云 , 李猷 , 陈倩 , 宾羽 , 杜宇 . 一种柑橘木虱源的抗菌肽DcAMP2的制备和应用 : CN202510699670.5. | 2025-05-28 .
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抗病毒肽及其防治水稻病毒病的应用 ipsunlight
专利 | 2025-03-21 | CN202510341064.6
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本发明公开了一种抗病毒肽及其防治水稻病毒病的应用。本发明打破了从土壤微生物寻找抗病毒肽的传统思路,创新性地从动物生物体内寻找可能的抗病毒肽;经一系列前期研究,最终确定了8条抗病毒肽序列,经验证,本发明提供的天然来源的抗病毒肽均对水稻病毒病有显著的防治作用,为水稻病毒病的防治提供了一种新的农药,对环境无害,效果非常明显。

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GB/T 7714 魏太云 , 杜宇 , 李猷 et al. 抗病毒肽及其防治水稻病毒病的应用 : CN202510341064.6[P]. | 2025-03-21 .
MLA 魏太云 et al. "抗病毒肽及其防治水稻病毒病的应用" : CN202510341064.6. | 2025-03-21 .
APA 魏太云 , 杜宇 , 李猷 , 宾羽 , 陈倩 . 抗病毒肽及其防治水稻病毒病的应用 : CN202510341064.6. | 2025-03-21 .
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The serial grinding microscopy for 3D visualization and reconstruction of insect structures SCIE
期刊论文 | 2025 , 205 (1) | ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY
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Serial grinding microscopy (SGM) facilitates the 3D visualization of organisms and industrial samples across multiple domains. Despite the development of light microscopy using serial slicing techniques for 3D reconstruction of insect specimens, the serial grinding technique has not been explored in entomology. An improved SGM prototype with a standardized workflow was developed, enabling high-resolution 3D visualization and reconstruction of intricate insect structures. The methodology's efficacy was validated through comprehensive testing of insect specimens encompassing a wide range of cuticle types and sizes (e.g. beetles, ants, and fruit flies), as well as an insect fossil in amber. This prototype features the finest x-y plane resolution and a z-axis step distance of 1 mu m. Further exploration of this technique may uncover its potential as a crucial research and diagnostic tool in zoology, other biological domains, and even non-biological materials such as industrial components.

Keyword :

functional morphology functional morphology micro-CT micro-CT microscopy microscopy sequential grinding sequential grinding serial sectioning serial sectioning tomography tomography

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GB/T 7714 Ruan, Yongying , Zhang, Mengna , Kundrata, Robin et al. The serial grinding microscopy for 3D visualization and reconstruction of insect structures [J]. | ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY , 2025 , 205 (1) .
MLA Ruan, Yongying et al. "The serial grinding microscopy for 3D visualization and reconstruction of insect structures" . | ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY 205 . 1 (2025) .
APA Ruan, Yongying , Zhang, Mengna , Kundrata, Robin , Xie, Rui , Li, You , Ge, Siqin et al. The serial grinding microscopy for 3D visualization and reconstruction of insect structures . | ZOOLOGICAL JOURNAL OF THE LINNEAN SOCIETY , 2025 , 205 (1) .
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Insect antiviral Mx-like protein forms biomolecular condensates to promote viral capsid ubiquitinated degradation SCIE
期刊论文 | 2025 , 44 (8) | CELL REPORTS
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Insects transmit diverse arthropod-borne viruses. However, it remains largely unknown whether insect innate immune pathways regulate the expression of antiviral factors to mediate the degradation of viral capsids. Here, we discover that transcription factor Dorsal in leafhopper Toll immune pathway binds to the promoter of vertebrate homolog myxovirus resistance-like (MxL) protein to activate its transcription. MxL associates with two different viral particles, promoting ubiquitinated degradation of viral capsids via E3 ubiquitin ligase SIAH1. Notably, MxL forms biomolecular condensates to recruit capsid protein P8 of a destructive rice reovirus and SIAH1. The disordered N-terminal region of MxL is essential for condensate formation. The Arg residue at position 159 of MxL is essential for MxL-P8 interaction and P8 recruitment into condensates. Viral nonstructural protein Pns11 interacts with Dorsal, effectively blocking its nuclear translocation and attenuating antiviral MxL1 expression. Our finding represents the first evidence that MxLs in invertebrates possess broad-spectrum antiviral functions.

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GB/T 7714 Du, Yu , Li, You , Xiao, Yuqing et al. Insect antiviral Mx-like protein forms biomolecular condensates to promote viral capsid ubiquitinated degradation [J]. | CELL REPORTS , 2025 , 44 (8) .
MLA Du, Yu et al. "Insect antiviral Mx-like protein forms biomolecular condensates to promote viral capsid ubiquitinated degradation" . | CELL REPORTS 44 . 8 (2025) .
APA Du, Yu , Li, You , Xiao, Yuqing , Jia, Dongsheng , Wei, Taiyun . Insect antiviral Mx-like protein forms biomolecular condensates to promote viral capsid ubiquitinated degradation . | CELL REPORTS , 2025 , 44 (8) .
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A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China SCIE
期刊论文 | 2025 , 5652 (1) , 195-200 | ZOOTAXA
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A new ambrosia beetle species of Anisandrus Ferrari, 1867, namelyA. tuberculatus sp. nov., is described from Chongqing, Shaanxi and Sichuan of Western China. Descriptions and illustrations are provided based on specimens from type materials found in Yintiaoling Nature Reserve of Chongqing.

Keyword :

new records new records species discovery species discovery taxonomy taxonomy Xyleborine ambrosia Xyleborine ambrosia

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GB/T 7714 Lin, Wei , Smith, Sarah M. , Xu, Miaofeng et al. A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China [J]. | ZOOTAXA , 2025 , 5652 (1) : 195-200 .
MLA Lin, Wei et al. "A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China" . | ZOOTAXA 5652 . 1 (2025) : 195-200 .
APA Lin, Wei , Smith, Sarah M. , Xu, Miaofeng , Li, You . A new species of Anisandrus Ferrari, 1867 (Coleoptera: Curculionidae: Scolytinae: Xyleborini) from Western China . | ZOOTAXA , 2025 , 5652 (1) , 195-200 .
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Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies SCIE
期刊论文 | 2025 , 81 (10) , 6076-6094 | PEST MANAGEMENT SCIENCE
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Huanglongbing (HLB), one of the most destructive diseases of citrus, is caused by the fastidious Gram-negative alpha-proteobacteria bacterium Candidatus Liberibacter asiaticus (CLas) and vectored by the Asian citrus psyllid (ACP), Diaphorina citri. Currently, there are no highly effective management strategies for HLB. This review focuses on specific aspects of HLB research, including the global distribution of Candidatus Liberibacter species in citrus, HLB status in major citrus-producing countries and plant-pathogen interactions. The elucidation of virulence effectors used by CLas is explored, focusing on the molecular mechanisms that enable the pathogen to manipulate the host plant, and the insect vector ACP to evade their immune systems. Biotechnological approaches and possible management methods for minimizing HLB and ACP risks are reviewed. This review provides a summary of the CLas Sec-delivered effectors, CLas flagellar genes, tight adherence pilus, transcriptional regulators and facilitates the development of integrated and sustainable solutions to safeguard citrus cultivation. (c) 2025 Society of Chemical Industry.

Keyword :

biotechnological approaches biotechnological approaches CLas CLas Diaphorina citri Diaphorina citri host-pathogen interactions host-pathogen interactions management methods management methods virulence effectors virulence effectors

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GB/T 7714 Younas, Muhammad , Zheng, Zheng , Atiq, Muhammad et al. Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies [J]. | PEST MANAGEMENT SCIENCE , 2025 , 81 (10) : 6076-6094 .
MLA Younas, Muhammad et al. "Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies" . | PEST MANAGEMENT SCIENCE 81 . 10 (2025) : 6076-6094 .
APA Younas, Muhammad , Zheng, Zheng , Atiq, Muhammad , Wei, Taiyun , Li, You . Insights into the Huanglongbing pandemic focusing on transmission biology, virulence factors and multifaceted management strategies . | PEST MANAGEMENT SCIENCE , 2025 , 81 (10) , 6076-6094 .
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Insect dome receptor directly recognizes viral envelopes to activate JAK/STAT signaling and induce conserved antiviral viperin expression SCIE
期刊论文 | 2025 , 44 (8) | CELL REPORTS
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Insect Janus kinase/signal transducer and activator of transcription (JAK/STAT) pathway plays a crucial role in antiviral defense, yet how arboviruses activate this pathway remains unclear. Here, we reveal that outer capsid protein of a double-stranded RNA (dsRNA) arbovirus (rice gall dwarf virus, RGDV) directly binds the Dome receptor's extracellular domain, triggering receptor dimerization and JAK/STAT activation. Structural and mutational analyses pinpoint cysteine 743 in Dome as essential for viral recognition, establishing Dome as the first identified viral pattern recognition receptor (PRR) in insects capable of initiating JAK/ STAT signaling, a pathway previously thought to be exclusively cytokine dependent. Furthermore, we identify viperin as a transcriptional factor STAT-regulated antiviral effector with broad-spectrum activity against multiple rice viruses. Intriguingly, RGDV's nonstructural protein Pns11 competitively binds STAT, blocking its phosphorylation by JAK and nuclear translocation, thereby suppressing viperin expression. These findings elucidate how leafhopper Dome functions as a viral PRR and how arboviruses antagonize Dome-JAK-STAT signaling to promote persistent viral infection.

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GB/T 7714 Chen, Siyu , Tang, Yupeng , Zhang, Chunyu et al. Insect dome receptor directly recognizes viral envelopes to activate JAK/STAT signaling and induce conserved antiviral viperin expression [J]. | CELL REPORTS , 2025 , 44 (8) .
MLA Chen, Siyu et al. "Insect dome receptor directly recognizes viral envelopes to activate JAK/STAT signaling and induce conserved antiviral viperin expression" . | CELL REPORTS 44 . 8 (2025) .
APA Chen, Siyu , Tang, Yupeng , Zhang, Chunyu , Li, You , Jia, Dongsheng , Wei, Taiyun . Insect dome receptor directly recognizes viral envelopes to activate JAK/STAT signaling and induce conserved antiviral viperin expression . | CELL REPORTS , 2025 , 44 (8) .
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Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor SCIE
期刊论文 | 2025 , 49 (1) , 94-110 | PLANT CELL AND ENVIRONMENT
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Janus kinase/signal transducer and activator of transcription (JAK/STAT) immune response traditionally requires cytokine binding to receptors for activation; however, it remains unclear whether cytokine receptors function as direct pattern recognition receptors (PRRs) that recognise viral envelopes. In this study, we demonstrate that cytokine receptor Domeless (Dome) in leafhopper Recilia dorsalis directly recognises glycoprotein (G) of an important rice rhabdovirus. This binding induces Dome dimerisation, which initiates JAK/STAT pathway and leads to STAT-dependent expression of antiviral immune effector in R. dorsalis (RdIE1). RdIE1 suppresses viral replication by inhibiting the RNA-binding activity of viral large polymerase (L). Dome residue T659 is essential for G recognition and L residues E774 and D775 are required for RdIE1 target. Notably, virus-encoded phosphoprotein competitively inhibits the phosphorylation of STAT by casein kinase II (CK2 beta), thereby abrogating STAT-dependent transactivation of target genes. Our findings redefine Dome as a viral sensor and reveal a biphasic strategy that balances antiviral immunity with persistent viral infection in insect vectors.

Keyword :

antiviral immune effector antiviral immune effector JAK/STAT pathway JAK/STAT pathway pathogens pathogens persistent viral infection persistent viral infection rhabdovirus rhabdovirus signalling signalling

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GB/T 7714 Yu, Tingting , Li, You , Xu, Mengjia et al. Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 49 (1) : 94-110 .
MLA Yu, Tingting et al. "Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor" . | PLANT CELL AND ENVIRONMENT 49 . 1 (2025) : 94-110 .
APA Yu, Tingting , Li, You , Xu, Mengjia , Wan, Zhihao , Jia, Dongsheng , Wei, Taiyun . Rhabdovirus Glycoprotein Triggers Janus Kinase/Signal Transducer and Activator of Transcription Antiviral Immunity via Direct Recognition by Insect Dome Receptor . | PLANT CELL AND ENVIRONMENT , 2025 , 49 (1) , 94-110 .
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Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection SCIE
期刊论文 | 2025 , 16 (10) | MBIO
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Phloem-inhabiting unculturable bacterial pathogens are persistently transmitted by insect vectors. However, how they evade insect immune responses to ensure persistent transmission remains unknown. The important melanization immune response in insects is triggered by cleavage of prophenoloxidase (PPO) into active phenoloxidase (PO) via clip-domain serine proteases (CLIPs). Here, we demonstrate that infection of Candidatus Liberibacter asiaticus (CLas) in psyllid vectors activates the peptidoglycan recognition protein (PGRP)-CLIP1-CLIP4-PPO-PO signaling cascade to induce a mild melanization response, ensuring persistent CLas infection without causing significant insect fitness costs to the insect. A CLas-encoded secretory protein, SDE3230, directly interacts with PGRP and suppresses its activity in transducing this signaling cascade. CLIP4 cleaves PPO between arginine 125 and methionine 126 residues to active PO to induce melanization, and this cleavage pattern in psyllid is distinct from other insects. However, SDE3230 competitively binds to this cleavage site of PPO with CLIP4, thereby suppressing PPO effective cleavage. Collectively, these findings reveal the dual role of SDE3230 in facilitating the mild melanization response, benefitting persistent CLas infection and insect fitness.IMPORTANCEPsyllid-borne huanglongbing is the most destructive citrus disease worldwide, causing billions of dollars in annual production losses and threatening the entire citrus industry. Currently, the mechanism by which the causal agent Candidatus Liberibacter asiaticus (CLas) antagonizes psyllid innate immune responses to facilitate its coexistence with psyllid vectors is still unknown. Here, we report that CLas exploits the highly expressed secretory protein SDE3230 in psyllids to suppress the important melanization immune response in hemolymph via inhibiting the pattern recognition receptor PGRP activity and the cleavage of prophenoloxidase into active phenoloxidase by clip-domain serine proteases. The pattern of PPO cleavage is novel, and this process ultimately ensures persistent CLas infection and insect fitness. Our findings provide insights into how CLas has evolved novel strategies to evade the insect melanization response, thereby facilitating persistent CLas transmission.

Keyword :

Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus huanglongbing huanglongbing melanization melanization persistent infection persistent infection psyllid psyllid secretory protein secretory protein

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GB/T 7714 Li, You , Du, Yu , Ren, Dongsheng et al. Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection [J]. | MBIO , 2025 , 16 (10) .
MLA Li, You et al. "Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection" . | MBIO 16 . 10 (2025) .
APA Li, You , Du, Yu , Ren, Dongsheng , Bin, Yu , Chen, Qian , Wei, Taiyun . Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection . | MBIO , 2025 , 16 (10) .
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