Query：
学者姓名：吴明
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
Liquid-liquid phase separation (LLPS) regulates diverse biological functions by mediating the assembly of biomolecular condensates. However, it remains unclear how host LLPS is targeted by viruses during infection. Here we show that a plant bunyaviral protein, the disease-specific protein (SP) encoded by rice stripe virus (RSV), possesses phase separation potential through its N-terminal intrinsically disordered region 1 (IDR1). In vivo, however, SP does not form phase-separated biomolecular condensates independently but utilizes its phase separation properties to interfere with the phase separation of the SERRATE protein (SE), a key component of Dicing bodies essential for microRNA processing. By disrupting SE phase separation, SP inhibits D-body assembly and miRNA biogenesis. Our study demonstrates that a viral protein can modulate host microRNA processing by targeting LLPS, revealing a previously uncharacterized mechanism involved in viral infection strategies and miRNA biogenesis regulation in plants.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zou, Jing , Zhang, Shuai , Chen, Ying et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis [J]. | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| MLA | Zou, Jing et al. "A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis" . | NATURE COMMUNICATIONS 16 . 1 (2025) . |
| APA | Zou, Jing , Zhang, Shuai , Chen, Ying , He, Chun , Pan, Xin , Zhang, Yimin et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis . | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Rice, a major global food staple, is threatened by viral infections that hinder its growth and yield. We have recently shown that the virulence protein P3 of rice grassy stunt virus promotes pathogenesis by inducing proteasome-controlled degradation of the rice RNA polymerase IV (RNA Pol IV) protein NRPD1a controlled by the P3-interacting E3 ubiquitin ligase P3IP1. However, the underlying mechanisms remain elusive. In this study, we show that P3 acts as a virus-encoded transcription activator-like effector to upregulate transcription of somatic embryogenesis receptor kinase 4 (SERK4) by directly binding to its promoter. SERK4 phosphorylates P3IP1 and enhances RNA Pol IVa (NRPD1a) degradation following P3IP1-controlled ubiquitination, leading to attenuated antiviral defense in rice. Thus, our study finds a critical viral virulence strategy by encoding a transcription factor-like protein that activates a host kinase to promote proteasome-controlled degradation of NRPD1a, thereby disarming RNA-directed DNA methylation (RdDM) antiviral defense.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wu, Yuansheng , Zhao, Shanshan , Liu, Na et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity [J]. | DEVELOPMENTAL CELL , 2025 , 60 (17) : 2348-2362 . |
| MLA | Wu, Yuansheng et al. "A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity" . | DEVELOPMENTAL CELL 60 . 17 (2025) : 2348-2362 . |
| APA | Wu, Yuansheng , Zhao, Shanshan , Liu, Na , Wu, Fengling , Huang, Shiting , Li, Dongyuan et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity . | DEVELOPMENTAL CELL , 2025 , 60 (17) , 2348-2362 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
N REQUIREMENT GENE 1 (NRG1) is a pivotal player in the basal immune system and in Toll/Interleukin-1 receptor nucleotide-binding domain leucine-rich repeat (TNL)-mediated effector-triggered immunity (ETI), yet its transcriptional regulation remains poorly investigated. In this study, we identified NbWRKY7, a WRKY transcription factor that enhances both basal immunity and TNL-mediated ETI by regulating NRG1 transcription and accumulation. NbWRKY7 directly binds to the W-box cis-elements within the NRG1 promoter and induces its transcription. Silencing NbWRKY7 compromises basal defense and impairs TNL-mediated resistance to both viral and bacterial pathogens, as well as hypersensitive response-type programmed cell death (HR-PCD). Notably, effectors trigger resistosome formation to activate NRG1-dependent immune responses, which, in turn, further enhance NbWRKY7 transcription, thereby reinforcing NRG1 transcription. The WRKY7-NRG1 module orchestrates TNL-mediated immune responses through a positive feedback loop in Nicotiana benthamiana. Our findings provide a perspective on the regulatory mechanisms of NRG1 during basal and TNL-mediated immune responses, offering insights into the complex interplay of transcription factors in plant immunity. The transcription factor WRKY7-immunity protein NRG1 module forms a positive feedback loop that amplifies plant basal immunity and TNL receptor-mediated effector-triggered immunity.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wu, Ming , Zheng, Xiyin , Hu, Maohong et al. WRKY7 positively regulates plant immunity by transcriptionally activating N REQUIREMENT GENE 1 in Nicotiana benthamiana [J]. | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| MLA | Wu, Ming et al. "WRKY7 positively regulates plant immunity by transcriptionally activating N REQUIREMENT GENE 1 in Nicotiana benthamiana" . | PLANT PHYSIOLOGY 199 . 2 (2025) . |
| APA | Wu, Ming , Zheng, Xiyin , Hu, Maohong , Zhang, Danfeng , Lei, Xin , Han, Meng et al. WRKY7 positively regulates plant immunity by transcriptionally activating N REQUIREMENT GENE 1 in Nicotiana benthamiana . | PLANT PHYSIOLOGY , 2025 , 199 (2) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明涉及生物技术领域，尤其涉及OsAGO12蛋白及其编码基因表达在调控植物对水稻瘤矮病毒抗性中的应用。OsAGO12蛋白的氨基酸序列如SEQ ID NO.2所示，其编码基因OsAGO12的核苷酸序列如SEQ ID NO.1所示。本发明采用过表达和CRISPR/Cas9技术分别将OsAGO12基因在水稻中进行过表达或者进行敲除突变，结果发现，在水稻中过表达OsAGO12基因能够显著增强水稻对水稻瘤矮病毒的抗性。本发明为水稻改良育种提供了新的基因资源。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 吴建国 , 赵珊珊 , 吴明 et al. OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用 : CN202411115055.7[P]. | 2024-08-14 . |
| MLA | 吴建国 et al. "OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用" : CN202411115055.7. | 2024-08-14 . |
| APA | 吴建国 , 赵珊珊 , 吴明 , 张帅 . OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用 : CN202411115055.7. | 2024-08-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The primary objective of neoantigen vaccines is to elicit a robust anti-tumor immune response by generating neoantigen-specific T cells that can eradicate tumor cells. Despite substantial advancements in personalized neoantigen prediction using next-generation sequencing, machine learning, and mass spectrometry, challenges remain in efficiently expanding neoantigen-specific T cell populations in vivo. This challenge impedes the widespread clinical application of neoantigen vaccines. Nanovector-based neoantigen delivery systems have emerged as a promising solutions to this challenge. These nanovectors offer several advantages, such as enhanced stability, targeted intracellular delivery, sustained release, and improved antigen-presenting cell (APC) activation. Notably, they effectively deliver various neoantigen vaccine formulations (DC cell-based, synthetic long peptide (SLP)-based or DNA/mRNA-based) to APCs or T cells, thereby activating both CD4+ T and CD8+ T cells. This ultimately induces a specific anti-tumor immune response. This review focuses on recent innovations in neoantigen vaccine delivery vectors. We aim to identify optimal design parameters for vectors tailored to different neoantigen vaccine types, with an emphasis on enhancing the tumor microenvironment and stimulating the production of neoantigen-specific cytotoxic T cells. By maximizing the potential of these delivery systems, we aim to accelerate the clinical translation of neoantigen nanovaccines and advance cancer immunotherapy.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zheng, Youshi , Wang, Bing , Cai, Zhixiong et al. Tailoring nanovectors for optimal neoantigen vaccine efficacy [J]. | JOURNAL OF MATERIALS CHEMISTRY B , 2025 , 13 (13) : 4045-4058 . |
| MLA | Zheng, Youshi et al. "Tailoring nanovectors for optimal neoantigen vaccine efficacy" . | JOURNAL OF MATERIALS CHEMISTRY B 13 . 13 (2025) : 4045-4058 . |
| APA | Zheng, Youshi , Wang, Bing , Cai, Zhixiong , Lai, Zisen , Yu, Haijun , Wu, Ming et al. Tailoring nanovectors for optimal neoantigen vaccine efficacy . | JOURNAL OF MATERIALS CHEMISTRY B , 2025 , 13 (13) , 4045-4058 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Pea seed-borne mosaic virus (PSbMV) is a significant pathogen that affects the yield and quality of leguminous crops. In recent years, PSbMV has emerged as the dominant viral threat to legume cultivation in Yunnan Province, China. In this study, the complete genome of PSbMV-YN isolate (GenBank accession: PP746839) with a length of 9919 nucleotides excluding the poly(A) tail was obtained by segmented amplification combined with rapid amplification of cDNA ends (RACE). Phylogenomic analysis of full-length polyprotein-encoding nucleotide and predicted amino acid sequences revealed that PSbMV-YN clusters within the P1 pathotype clade. Genetic mapping of local pea varieties demonstrated eIF4E, a gene tightly linked to the sbm1 locus, as the susceptibility genotype, revealing the mechanistic basis for the prevalence of the P1 pathotype in Yunnan pea populations. Further analysis of the highly variable region of the P1 cistron in PSbMV-YN indicates that the P1 protein could be classified as Type A, which generally lacks RNA silencing suppressor activity. Using a GFP suppression assay, we further validated the RNA silencing suppressor activity of HC-Pro in both Nicotiana benthamiana and its natural host-Pisum sativum L. Additionally, immunoprecipitation sequencing (IP-seq) revealed that HC-Pro binds to small RNAs of variable lengths, thereby effectively inhibiting both local and systemic RNA silencing to promote viral infection. This study reports the first genomic sequence of a Chinese isolate of PSbMV and provides a comprehensive analysis of interactions between HC-Pro with endogenous small RNAs.
Keyword ：
eIF4E eIF4E HC-pro HC-pro Immunoprecipitation sequencing Immunoprecipitation sequencing Pisum sativum L. Pisum sativum L. PSbMV PSbMV
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Ma, Junfeng , Wu, Ming , Dong, Chaoyi et al. Molecular characterization of pea seed-borne mosaic virus and the RNA silencing suppression mechanism of HC-Pro [J]. | PHYTOPATHOLOGY RESEARCH , 2025 , 7 (1) . |
| MLA | Ma, Junfeng et al. "Molecular characterization of pea seed-borne mosaic virus and the RNA silencing suppression mechanism of HC-Pro" . | PHYTOPATHOLOGY RESEARCH 7 . 1 (2025) . |
| APA | Ma, Junfeng , Wu, Ming , Dong, Chaoyi , Huang, Liandi , Cheng, Yabing , Hu, Qun et al. Molecular characterization of pea seed-borne mosaic virus and the RNA silencing suppression mechanism of HC-Pro . | PHYTOPATHOLOGY RESEARCH , 2025 , 7 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wu, Ming , Zhao, Shanshan , Wu, Jianguo . Simple but effective: Minimalist NLR rewiring, maximal virus resistance [J]. | MOLECULAR PLANT , 2025 , 18 (11) : 1803-1805 . |
| MLA | Wu, Ming et al. "Simple but effective: Minimalist NLR rewiring, maximal virus resistance" . | MOLECULAR PLANT 18 . 11 (2025) : 1803-1805 . |
| APA | Wu, Ming , Zhao, Shanshan , Wu, Jianguo . Simple but effective: Minimalist NLR rewiring, maximal virus resistance . | MOLECULAR PLANT , 2025 , 18 (11) , 1803-1805 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Viral pathogens not only threaten the health and life of humans and animals but also cause enormous crop yield losses and contribute to global food insecurity. To defend against viral pathogens, plants have evolved an intricate immune system to perceive and cope with such attacks. Although most of the fundamental studies were carried out in model plants, more recent research in crops has provided new insights into the antiviral strategies employed by crop plants. We summarize recent advances in understanding the biological roles of cellular receptors, RNA silencing, RNA decay, hormone signaling, autophagy, and ubiquitination in manipulating crop host-mediated antiviral responses. The potential functions of circular RNAs, the rhizosphere microbiome, and the foliar microbiome of crops in plant-virus interactions will be fascinating research directions in the future. These findings will be beneficial for the development of modern crop improvement strategies.
Keyword ：
antiviral defense antiviral defense autophagy autophagy crops crops RNA silencing RNA silencing ubiquitin proteasome system ubiquitin proteasome system
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Yang, Zhirui , Li, Guangyao , Zhang, Yongliang et al. Crop antiviral defense: Past and future perspective [J]. | SCIENCE CHINA-LIFE SCIENCES , 2024 , 67 (12) : 2617-2634 . |
| MLA | Yang, Zhirui et al. "Crop antiviral defense: Past and future perspective" . | SCIENCE CHINA-LIFE SCIENCES 67 . 12 (2024) : 2617-2634 . |
| APA | Yang, Zhirui , Li, Guangyao , Zhang, Yongliang , Li, Fangfang , Zhou, Tao , Ye, Jian et al. Crop antiviral defense: Past and future perspective . | SCIENCE CHINA-LIFE SCIENCES , 2024 , 67 (12) , 2617-2634 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了水稻AGO12基因及其编码蛋白在控制水稻籽粒大小和产量中的应用，本发明方案通过实验发现，在水稻中过表达OsAGO12基因可增加水稻籽粒的长度和宽度，提高水稻籽粒大小和千粒重，从而增加水稻产量。OsAGO12蛋白质可通过调控籽粒外颖壳细胞的大小的，进而实现增加植物籽粒大小、提高植物产量。OsAGO12蛋白质在培育大粒高产植物品种中具有重大的应用价值。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 吴建国 , 赵珊珊 , 吴明 et al. 水稻AGO12基因及其编码蛋白在控制水稻籽粒大小和产量中的应用 : CN202410492090.4[P]. | 2024-04-23 . |
| MLA | 吴建国 et al. "水稻AGO12基因及其编码蛋白在控制水稻籽粒大小和产量中的应用" : CN202410492090.4. | 2024-04-23 . |
| APA | 吴建国 , 赵珊珊 , 吴明 , 张帅 . 水稻AGO12基因及其编码蛋白在控制水稻籽粒大小和产量中的应用 : CN202410492090.4. | 2024-04-23 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
本发明公开了OsAGO17蛋白及其编码基因在提高水稻对南方水稻黑条矮缩病毒抗性中的应用，属于生物技术领域。本发明首次研究发现，过表达OsAGO17蛋白或提升编码OsAGO17蛋白的核酸分子的表达量，水稻对南方水稻黑条矮缩病毒的抗性增加。本发明可用于水稻种质资源的改良及遗传育种领域，具有良好的市场应用前景。
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | 吴建国 , 赵珊珊 , 吴明 et al. OsAGO17蛋白及其编码基因在提高植物对南方水稻黑条矮缩病毒抗性中的应用 : CN202411115211.X[P]. | 2024-08-14 . |
| MLA | 吴建国 et al. "OsAGO17蛋白及其编码基因在提高植物对南方水稻黑条矮缩病毒抗性中的应用" : CN202411115211.X. | 2024-08-14 . |
| APA | 吴建国 , 赵珊珊 , 吴明 , 张帅 . OsAGO17蛋白及其编码基因在提高植物对南方水稻黑条矮缩病毒抗性中的应用 : CN202411115211.X. | 2024-08-14 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
