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学者姓名：陈凤平
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Abstract ：
Utilizing nanotechnology for pesticide delivery can enhance their uptake and distribution in plants, thereby boosting pesticide efficiency. Fluazinam, a highly effective broad-spectrum fungicide with unique mode of action, is hindered by its poor systemic conductivity, limiting its field control efficacy. In this study, fluazinam-loaded nanocapsules were developed, which exhibited significantly higher inhibitory activity against various Phytophthora species compared to the commercial suspension concentrate. Notably, the nanocapsules demonstrated pronounced effects on reducing ATP content in Phytophthora capsici and effectively controlled pepper blight and cucumber downy mildew. Tracking the distribution of fluorescein isothiocyanate (FITC) fluorescein-labeled fluazinam nanocapsules revealed their presence in roots seven days after treatment. High-performance liquid chromatography (HPLC) analysis confirmed the easy absorption and transport of fluazinam nanocapsules by pepper plants. Moreover, treatment with fluazinam nanocapsules significantly increased the expression of two exocytosis genes, FACP and RABF2a, in pepper tissues. Lastly, fluazinam nanocapsules were found to enhance the relative abundance of beneficial bacteria in the soil microbial community. This study presents a promising approach to enhance the upward transport capability of non-systemic pesticides, offering novel insights into their application and improved efficacy.
Keyword ：
Microbial communities Microbial communities Nanocapsules Nanocapsules Non-systemic pesticides Non-systemic pesticides Soil application Soil application Upward conduction Upward conduction
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| GB/T 7714 | Xiao, Wenhui , Du, Jiang , Zheng, Yuxin et al. Nanocarriers boost non-systemic fluazinam transportation in plants and microbial community enrichment in soil [J]. | JOURNAL OF NANOBIOTECHNOLOGY , 2025 , 23 (1) . |
| MLA | Xiao, Wenhui et al. "Nanocarriers boost non-systemic fluazinam transportation in plants and microbial community enrichment in soil" . | JOURNAL OF NANOBIOTECHNOLOGY 23 . 1 (2025) . |
| APA | Xiao, Wenhui , Du, Jiang , Zheng, Yuxin , Chen, Shanshan , Liu, Zhanyun , Chen, Fengping et al. Nanocarriers boost non-systemic fluazinam transportation in plants and microbial community enrichment in soil . | JOURNAL OF NANOBIOTECHNOLOGY , 2025 , 23 (1) . |
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Monilinia fructicola is one of main pathogens causing brown rot in peach. Fungicide applications are the most practical way to manage this disease still now, however, overuse of fungicide has led to pathogen resistance. In this study, the susceptibility of 87 field strains of M. fructicola to iprodione was investigated. The results showed that two of them were resistant confirmed by fruit assay of iprodione application. Further investigation showed higher osmotic sensitivity in the resistant strains. Therefore, the four whole osmosensing two-component histidine kinase genes (MfOs-1, MfOs-2, MfOs-4 and MfOs-5) were amplified and the corresponding amino acid sequences comparison showed that two-point mutations in MfOs-1 associated with iprodione resistance. One of the two mutations is with alanine replaced by valine at 307 position (A307V) and another is glycine replaced by serine at 462 position (G462S). Further fitness parameters investigation showed penalty in resistant strains indicating by reduced mycelial growth rate, less sporulation, and smaller lesion size on detached fruit. This is the first report of M. fructicola field strains resistant to dicarboximide fungicides. The results obtained in this study will provide a basis for effectively control the spread of brown rot.
Keyword ：
DCF fungicide DCF fungicide Fungicide resistance Fungicide resistance Monilinia fructicola Monilinia fructicola Os-1 Os-1 Point mutation Point mutation
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| GB/T 7714 | Long, Qian , Guo, Yiyi , Yi, Miaomiao et al. New point mutations in osmosensing two-component histidine kinase gene associated with iprodione resistance in Monilinia fructicola field isolates from China [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 213 . |
| MLA | Long, Qian et al. "New point mutations in osmosensing two-component histidine kinase gene associated with iprodione resistance in Monilinia fructicola field isolates from China" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 213 (2025) . |
| APA | Long, Qian , Guo, Yiyi , Yi, Miaomiao , Lu, Changsheng , Zhong, Rui , Zhang, Hao et al. New point mutations in osmosensing two-component histidine kinase gene associated with iprodione resistance in Monilinia fructicola field isolates from China . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 213 . |
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【目的】为评价马铃薯种质资源对马铃薯黑胫病的抗性及筛选抗病种质，以期为抗病育种及田间病害防治提供抗性材料和理论依据。【方法】以45份马铃薯种质资源为材料，采用离体薯块接种鉴定方法，通过优化接种条件，评价其对马铃薯黑胫病菌（Pectobacterium atrosepticum和P. parmentieri）的抗性。【结果】2种马铃薯黑胫病菌病原菌优化后的接种条件为培养温度26℃，接种浓度D
Keyword ：
优化接种条件 优化接种条件 抗性评价 抗性评价 抗病种质 抗病种质 马铃薯 马铃薯 黑胫病 黑胫病
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| GB/T 7714 | 易苗苗 , 李华伟 , 鲍宗朝 et al. 马铃薯种质资源黑胫病抗性评价与抗病种质筛选 [J]. | 东南园艺 , 2025 , 13 (02) : 175-181 . |
| MLA | 易苗苗 et al. "马铃薯种质资源黑胫病抗性评价与抗病种质筛选" . | 东南园艺 13 . 02 (2025) : 175-181 . |
| APA | 易苗苗 , 李华伟 , 鲍宗朝 , 许泳清 , 许国春 , 罗文彬 et al. 马铃薯种质资源黑胫病抗性评价与抗病种质筛选 . | 东南园艺 , 2025 , 13 (02) , 175-181 . |
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为探明β-微管蛋白抑制剂对抗药水平不同的桃褐腐病菌的抑制作用，测定了3种β-微管蛋白抑制剂甲基硫菌灵、多菌灵、乙霉威对桃褐腐病菌（2株对甲基硫菌灵表现敏感，2株表现抗性）菌丝生长、产孢、分生孢子萌发、芽管伸长等不同阶段的作用，结果表明：甲基硫菌灵对供试菌株的菌丝生长和产孢均有一定的抑制效果，对分生孢子萌发和芽管伸长的抑制效果较差；多菌灵在供试菌株各生长发育阶段的抑制作用与甲基硫菌灵大致相近，但在菌丝生长阶段多菌灵对抗药菌株的抑制效果略优于甲基硫菌灵，在产孢阶段甲基硫菌灵浓度达到5μg/mL时对抗药菌株的抑制作用优于多菌灵同浓度处理；乙霉威在供试菌株各生长发育阶段表现出的抑制效果较弱。本研究结果对精准设计褐腐病用药方案具有一定的参考意义。
Keyword ：
β-微管蛋白抑制剂 β-微管蛋白抑制剂 抑制活性 抑制活性 桃褐腐病菌 桃褐腐病菌 生长发育阶段 生长发育阶段
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| GB/T 7714 | 郭一祎 , 钟锐 , 龙倩 et al. 不同β-微管蛋白抑制剂对桃褐腐病菌的抑制作用探究 [J]. | 中国植保导刊 , 2025 , 45 (02) : 5-10 . |
| MLA | 郭一祎 et al. "不同β-微管蛋白抑制剂对桃褐腐病菌的抑制作用探究" . | 中国植保导刊 45 . 02 (2025) : 5-10 . |
| APA | 郭一祎 , 钟锐 , 龙倩 , 邓升 , 祝雯 , 陈凤平 . 不同β-微管蛋白抑制剂对桃褐腐病菌的抑制作用探究 . | 中国植保导刊 , 2025 , 45 (02) , 5-10 . |
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The development of gold nanobipyramid (AuNBP)-based multicolor colorimetric aptasensors offers significant advantages for enabling sensitive, accurate, and visual on-site detection. However, existing AuNBP growth-based strategies suffer from the inherent limitations of enzymes and the instability of AuNBPs that restrict their application in aptasensor construction. In this study, a multicolor colorimetric aptasensor based on a novel Gquadruplex (G4) DNAzyme-mediated AuNBP growth strategy was developed for the detection of quinclorac (QNC), a prevalent acid herbicide. First, complexes of magnetic bead (MB)-QNC aptamer (Qapt)-G4 DNA were formed through an MB-assisted competitive aptamer-binding system. In this system, the amount of MB-aptamer-G4 DNA complexes formed was directly proportional to the QNC concentration. These complexes subsequently facilitated the G4 DNAzyme-catalyzed oxidation of hydroquinone (HQ). The remaining HQ was then utilized to grow AuNBPs, leading to distinct multicolor variations. Under optimal conditions, the aptasensor exhibited nine discernible color changes corresponding to different QNC concentrations with a visual limit of detection (LOD) of 0.5 mu M and an instrumental LOD of 113 nM. Moreover, the aptasensor demonstrated excellent specificity and color stability. It was successfully used to determine QNC in river water, soil, and rice samples with recovery rates of 95.20 %-100.69 % and relative standard deviations (n = 5) < 3 %. These results indicate that it is a reliable and promising method for the rapid screening of QNC residues in environmental and food samples. Furthermore, this work provides a valuable reference strategy for transforming G4 DNAzyme-based colorimetric aptasensors into multicolor analytical platforms.
Keyword ：
Gold nanobipyramid growth Gold nanobipyramid growth G-quadruplex DNAzyme G-quadruplex DNAzyme Hydroquinone Hydroquinone Multicolor aptasensor Multicolor aptasensor Visual detection Visual detection
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| GB/T 7714 | Wang, Zemiao , Hu, Yang , Liu, Jiayi et al. A multicolor colorimetric aptasensor for visual detection of quinclorac based on G-quadruplex DNAzyme-mediated gold nanobipyramid growth [J]. | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 348 . |
| MLA | Wang, Zemiao et al. "A multicolor colorimetric aptasensor for visual detection of quinclorac based on G-quadruplex DNAzyme-mediated gold nanobipyramid growth" . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 348 (2025) . |
| APA | Wang, Zemiao , Hu, Yang , Liu, Jiayi , Luo, Guizhen , He, Xue , Chen, Fengping et al. A multicolor colorimetric aptasensor for visual detection of quinclorac based on G-quadruplex DNAzyme-mediated gold nanobipyramid growth . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 348 . |
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Sucrose non-fermenting-1-related protein kinase-1 (SnRK1) is a highly conserved serine-threonine kinase complex regulating plants' energy metabolisms and resistance to various types of stresses. However, the downstream genes regulated by SnRK1 in these plant physiological processes still need to be explored. In this study, we found that the knockout of OsSnRK1a resulted in no obvious defects in rice growth but notably decreased the seed setting rate. The ossnrk1a mutants were more sensitive to blast fungus (Magnaporthe oryzae) infection and showed compromised immune responses. Transcriptome analyses revealed that SnRK1a was an important intermediate in the energy metabolism and response to biotic stress. Further investigation confirmed that the transcription levels of OsNADH-GOGAT2, which positively controls rice yield, and the defense-related gene pathogenesis-related protein 1b (OsPR1b) were remarkably decreased in the ossnrk1a mutant. Moreover, we found that OsSnRK1a directly interacted with the regulatory subunits OsSnRK1 beta 1 and OsSnRK1 beta 3, which responded specifically to blast fungus infection and starvation stresses, respectively. Taken together, our findings provide an insight into the mechanism of OsSnRK1a, which forms a complex with specific beta subunits, contributing to rice seed set and resistance by regulating the transcription of related genes.
Keyword ：
blast fungus blast fungus gene expression gene expression OsSnRK1a OsSnRK1a resistance resistance rice rice seed set seed set
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| GB/T 7714 | Cao, Yingying , Lu, Minfeng , Chen, Jinhui et al. Identification of Ossnrk1a-1 Regulated Genes Associated with Rice Immunity and Seed Set [J]. | PLANTS-BASEL , 2024 , 13 (5) . |
| MLA | Cao, Yingying et al. "Identification of Ossnrk1a-1 Regulated Genes Associated with Rice Immunity and Seed Set" . | PLANTS-BASEL 13 . 5 (2024) . |
| APA | Cao, Yingying , Lu, Minfeng , Chen, Jinhui , Li, Wenyan , Wang, Mo , Chen, Fengping . Identification of Ossnrk1a-1 Regulated Genes Associated with Rice Immunity and Seed Set . | PLANTS-BASEL , 2024 , 13 (5) . |
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Quinone outside inhibitor (QoI) has been applied to manage taro leaf blight caused by Phytophthora colocasiae in southeastern of China for many years. The risk of P. colocasiae to QoI and the potential resistant mechanism remain unknown. In this study, the 74 P. colocasiae strains were sampled from southeastern of China. Sequence analysis of the QoI target Cytb showed one nucleotide variant in the fragment of this gene in this population, producing two haplotypes. The nucleotide variant leads to codon change at 142 (GGT to GCT) producing A142 (alanine) and G142 (glycine) in Hap_1 and Hap_2 strains, respectively. The sensitivity differentiation to azoxystrobin of two haplotypes were observed in vitro. The Hap_1 and Hap_2 strains were confirmed resistant and sensitive by control efficacy of label rate fungicide application, which was 3.0% and 88.8% treated with 500 mu g/ mL azoxystrobin, respectively. In addition, 10.0 mu g/mL azoxystrobin plus 50 mu g/mL salicylhydroxamic acid (SHAM) supplemented in PDA medium was identified as a discriminatory dose for differentiation of these two phenotype strains. The azoxystrobin resistant frequency reached 86.5%, indicating prevalence of QoI resistance in the field. Further fitness related features showed that no significant difference in temperature sensitivity, mycelial growth rate, sporangia production, zoospore release and aggressiveness between azoxystrobin-resistant and sensitive strains indicating no potential fitness cost for azoxystrobin resistance. Taken together, azoxystrobin resistance need to be taken into consideration to manage taro leaf blight in southeastern of China.
Keyword ：
Cytochrome b Cytochrome b G142A G142A Phytophthora colocasiae Phytophthora colocasiae Quinone outside inhibitor Quinone outside inhibitor
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| GB/T 7714 | Wang, Jinmiao , Chen, Songyu , Zhou, Ying et al. High resistance risk of Phytophthora colocasiae to azoxystrobin in southeastern of China [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2024 , 202 . |
| MLA | Wang, Jinmiao et al. "High resistance risk of Phytophthora colocasiae to azoxystrobin in southeastern of China" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 202 (2024) . |
| APA | Wang, Jinmiao , Chen, Songyu , Zhou, Ying , Peng, Qin , Shi, Niuniu , Du, Yixin et al. High resistance risk of Phytophthora colocasiae to azoxystrobin in southeastern of China . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2024 , 202 . |
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The lack of inherent structure -switching functionality and inadequate affinity in aptamers hinders the construction and application of aptasensors. While exonuclease digestion -based strategies have been employed to engineer small -molecule aptamers with the structure -switching functionality and construct dual-exonuclease (exonuclease I and exonuclease III) digestion -based aptasensors, such studies targeting G-quadruplex (G4) aptamers remain scarce. This study focused on the exonuclease digestion and circular dichroism spectroscopy analysis of a G4 quinclorac (QNC) aptamer, Qapt-51, intending to design a functionalized QNC aptamer and a universal signal amplification strategy for constructing folding -based and sensitive dual-exonuclease digestionbased aptasensors, respectively. The exonuclease digestion results demonstrated that QNC-binding Qapt-51 inhibited the dual-exonuclease digestion. Combining the results from the circular dichroism spectroscopy, we further proposed a digestion mechanism for Qapt-51 involving a G4 conformational switch. Inspired by this mechanism, a functionalized Qapt-51 variant (Qapt-45A) was rationally designed to construct a simple and rapid folding -based fluorescence aptasensor. Additionally, we developed an exonuclease III- and phosphorodiamidate morpholino oligomer-assisted fluorescence signal amplification strategy, which can work in the dual-exonuclease digestion system. Leveraging this strategy integrated with the dual-exonuclease digestion of another Qapt-51 variant (AQ), a homogeneous signal -amplified fluorescence aptasensor was constructed to sensitively detect QNC without requiring the functionalized aptamers. These two aptasensors achieved detection limits of 560 ng/ mL and 8.5 ng/mL for QNC. They successfully detected QNC in rice and river water samples with recoveries of 108.6%-87.5%. We hope that the successful development of these two aptasensors can provide valuable insights and references for the broader construction of G4 aptamer-based sensing systems.
Keyword ：
Exonuclease I Exonuclease I Exonuclease III Exonuclease III N -methyl mesoporphyrin IX N -methyl mesoporphyrin IX Phosphorodiamidate morpholino oligomer Phosphorodiamidate morpholino oligomer Recycling amplification Recycling amplification
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| GB/T 7714 | Wang, Zemiao , Zhang, Feng , Fang, Ling et al. Development of folding-based and signal-amplified fluorescence aptasensors using G-quadruplex quinclorac aptamer variants engineered via exonuclease digestion and circular dichroism spectroscopy [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 415 . |
| MLA | Wang, Zemiao et al. "Development of folding-based and signal-amplified fluorescence aptasensors using G-quadruplex quinclorac aptamer variants engineered via exonuclease digestion and circular dichroism spectroscopy" . | SENSORS AND ACTUATORS B-CHEMICAL 415 (2024) . |
| APA | Wang, Zemiao , Zhang, Feng , Fang, Ling , Chen, Fengping , Yang, Weijuan , Wang, Zongwen . Development of folding-based and signal-amplified fluorescence aptasensors using G-quadruplex quinclorac aptamer variants engineered via exonuclease digestion and circular dichroism spectroscopy . | SENSORS AND ACTUATORS B-CHEMICAL , 2024 , 415 . |
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Ferredoxins (Fds) in plastids are the most upstream stromal electron receptors shuttling electrons to downstream metabolic systems and function in various physiological processes of dicots, but their roles in monocots' response to stresses are still unclear. In this study, the functions of OsFd4, the major non-photosynthetic type Fd in rice, were characterized under oxidative stress and Xanthomonas oryzae pv. oryzae (Xoo) infection. OsFd4-knockout mutants displayed no defects in key agronomic traits and blast resistance, but were more sensitive to hydrogen peroxide (H2O2) treatment than the wild type. Transient expression of OsFd4 alleviated H2O2-induced rice cell death, suggesting that OsFd4 contributes to rice tolerance to exogenous oxidative stress. Deletion of OsFd4 enhanced rice immune responses against Xoo. OsFd4 formed a complex in vivo with itself and OsFd1, the major photosynthetic Fd in rice, and OsFd1 transcripts were increased in leaf and root tissues of the OsFd4-knockout mutants. These results indicate that OsFd4 is involved in regulating rice defense against stresses and interplays with OsFd1. (c) 2023 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Keyword ：
Blight bacteria Blight bacteria Ferredoxin Ferredoxin OsFd4 OsFd4 Oxidative stress Oxidative stress Rice Rice
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| GB/T 7714 | Lu, Minfeng , Chen, Jinhui , Meng, Han et al. Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria [J]. | CROP JOURNAL , 2023 , 11 (6) : 1937-1942 . |
| MLA | Lu, Minfeng et al. "Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria" . | CROP JOURNAL 11 . 6 (2023) : 1937-1942 . |
| APA | Lu, Minfeng , Chen, Jinhui , Meng, Han , Mo, Guangling , Liu, Yunhong , Chen, Fengping et al. Rice ferredoxin OsFd4 contributes to oxidative stress tolerance but compromises defense against blight bacteria . | CROP JOURNAL , 2023 , 11 (6) , 1937-1942 . |
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Fujian Fujian M. mumecola M. mumecola plum plum southeast coast of China southeast coast of China
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| GB/T 7714 | Lei, Wenting , Zhang, Hao , Zheng, Yulong et al. First Report of Brown Rot Caused by Monilia mumecola on Chinese Plum (Prunus salicina) in Fujian, China [J]. | PLANT DISEASE , 2023 , 107 (9) . |
| MLA | Lei, Wenting et al. "First Report of Brown Rot Caused by Monilia mumecola on Chinese Plum (Prunus salicina) in Fujian, China" . | PLANT DISEASE 107 . 9 (2023) . |
| APA | Lei, Wenting , Zhang, Hao , Zheng, Yulong , Ke, Dufang , Wang, Mingyi , Lin, Sheng et al. First Report of Brown Rot Caused by Monilia mumecola on Chinese Plum (Prunus salicina) in Fujian, China . | PLANT DISEASE , 2023 , 107 (9) . |
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