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学者姓名：李毅
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Abstract ：
Crop production faces persistent threats from insect-vector-borne viral diseases1,2. Recent advancements have revealed the intricate immune mechanisms that plants deploy against viral pathogens3, 4, 5, 6, 7-8. However, the molecular mechanisms through which plant hosts recognize viral infections and initiate antiviral defence at disease onset have not been elucidated. Here, through the natural infection of rice by inoculation with insect vectors carrying the natural forms of viruses, we show that viral coat proteins are perceived by the RING1-IBR-RING2-type ubiquitin ligase (RBRL), initiating the first step of the natural antiviral response in rice. RBRL subsequently targets an adaptor protein of the transcriptional repression complex of the jasmonate pathway, NOVEL INTERACTOR OF JAZ 3 (NINJA3), for degradation through the ubiquitination system, inducing jasmonate signalling and activating downstream antiviral defence. We further show that this phenomenon is a universal molecular mechanism used by rice plants to perceive viral infections and initiate antiviral signalling cascades. This approach is important not only for obtaining a deeper understanding of virus-host interactions but also for further disease resistance breeding.
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| GB/T 7714 | Huang, Yu , Yang, Jialin , Sun, Xi et al. Perception of viral infections and initiation of antiviral defence in rice [J]. | NATURE , 2025 , 641 (8061) . |
| MLA | Huang, Yu et al. "Perception of viral infections and initiation of antiviral defence in rice" . | NATURE 641 . 8061 (2025) . |
| APA | Huang, Yu , Yang, Jialin , Sun, Xi , Li, Jiahao , Cao, Xiaoqiang , Yao, Shengze et al. Perception of viral infections and initiation of antiviral defence in rice . | NATURE , 2025 , 641 (8061) . |
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Liquid-liquid phase separation (LLPS) regulates diverse biological functions by mediating the assembly of biomolecular condensates. However, it remains unclear how host LLPS is targeted by viruses during infection. Here we show that a plant bunyaviral protein, the disease-specific protein (SP) encoded by rice stripe virus (RSV), possesses phase separation potential through its N-terminal intrinsically disordered region 1 (IDR1). In vivo, however, SP does not form phase-separated biomolecular condensates independently but utilizes its phase separation properties to interfere with the phase separation of the SERRATE protein (SE), a key component of Dicing bodies essential for microRNA processing. By disrupting SE phase separation, SP inhibits D-body assembly and miRNA biogenesis. Our study demonstrates that a viral protein can modulate host microRNA processing by targeting LLPS, revealing a previously uncharacterized mechanism involved in viral infection strategies and miRNA biogenesis regulation in plants.
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| GB/T 7714 | Zou, Jing , Zhang, Shuai , Chen, Ying et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis [J]. | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| MLA | Zou, Jing et al. "A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis" . | NATURE COMMUNICATIONS 16 . 1 (2025) . |
| APA | Zou, Jing , Zhang, Shuai , Chen, Ying , He, Chun , Pan, Xin , Zhang, Yimin et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis . | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
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Rice, a major global food staple, is threatened by viral infections that hinder its growth and yield. We have recently shown that the virulence protein P3 of rice grassy stunt virus promotes pathogenesis by inducing proteasome-controlled degradation of the rice RNA polymerase IV (RNA Pol IV) protein NRPD1a controlled by the P3-interacting E3 ubiquitin ligase P3IP1. However, the underlying mechanisms remain elusive. In this study, we show that P3 acts as a virus-encoded transcription activator-like effector to upregulate transcription of somatic embryogenesis receptor kinase 4 (SERK4) by directly binding to its promoter. SERK4 phosphorylates P3IP1 and enhances RNA Pol IVa (NRPD1a) degradation following P3IP1-controlled ubiquitination, leading to attenuated antiviral defense in rice. Thus, our study finds a critical viral virulence strategy by encoding a transcription factor-like protein that activates a host kinase to promote proteasome-controlled degradation of NRPD1a, thereby disarming RNA-directed DNA methylation (RdDM) antiviral defense.
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| GB/T 7714 | Wu, Yuansheng , Zhao, Shanshan , Liu, Na et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity [J]. | DEVELOPMENTAL CELL , 2025 , 60 (17) : 2348-2362 . |
| MLA | Wu, Yuansheng et al. "A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity" . | DEVELOPMENTAL CELL 60 . 17 (2025) : 2348-2362 . |
| APA | Wu, Yuansheng , Zhao, Shanshan , Liu, Na , Wu, Fengling , Huang, Shiting , Li, Dongyuan et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity . | DEVELOPMENTAL CELL , 2025 , 60 (17) , 2348-2362 . |
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Rice (Oryza sativa), a staple crop sustaining half of humanity's caloric intake, is threatened by numerous insect-vectortransmitted diseases, such as rice stripe disease, caused by the rice stripe virus (RSV). Most genetic studies on plant antiviral defense mechanisms rely on natural or artificial infection and transgenic approaches, which require months of plant transformation. Here, we present a streamlined protocol that enables rapid analysis of RSV-host interactions within three days. The method encompasses three key phases: (1) polyethylene glycol (PEG)-based precipitation of RSV virions from infected plant tissues, (2) sequential purification through differential ultracentrifugation with glycerol cushion optimization, and (3) high-efficiency transfection of purified virions into rice protoplasts via PEG-mediated delivery. Viral replication is quantitatively assessed using RT-qPCR targeting viral RNA and immunoblotting with RSV nucleocapsid protein-specific monoclonal antibodies. This approach eliminates dependency on stable transgenic lines, allowing the simultaneous introduction of exogenous plasmids for functional studies. Compared with conventional methods requiring several months for transgenic plant generation, our protocol delivers analyzable results within three days, significantly accelerating the exploration of antiviral mechanisms and resistance gene screening.
Keyword ：
Protoplast isolation Protoplast isolation Protoplast transfection Protoplast transfection Rice Rice Rice stripe virus Rice stripe virus Ultracentrifugation Ultracentrifugation Virion purification Virion purification
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| GB/T 7714 | Huang, Yu , Yang, Zhirui , Li, Yi . Virus Isolation and Rice Protoplast Infection [J]. | BIO-PROTOCOL , 2025 , 15 (14) . |
| MLA | Huang, Yu et al. "Virus Isolation and Rice Protoplast Infection" . | BIO-PROTOCOL 15 . 14 (2025) . |
| APA | Huang, Yu , Yang, Zhirui , Li, Yi . Virus Isolation and Rice Protoplast Infection . | BIO-PROTOCOL , 2025 , 15 (14) . |
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| GB/T 7714 | Song, Shuangwei , Wang, Qian , Huo, Liangxiao et al. The phytoplasma (Candidatus Phytoplasma arecae) is the crucial pathogen to cause areca palm yellow leaf disease [J]. | SCIENCE BULLETIN , 2025 , 70 (6) : 847-851 . |
| MLA | Song, Shuangwei et al. "The phytoplasma (Candidatus Phytoplasma arecae) is the crucial pathogen to cause areca palm yellow leaf disease" . | SCIENCE BULLETIN 70 . 6 (2025) : 847-851 . |
| APA | Song, Shuangwei , Wang, Qian , Huo, Liangxiao , Xie, Liqiang , Chen, Jiaqi , Cui, Hongguang et al. The phytoplasma (Candidatus Phytoplasma arecae) is the crucial pathogen to cause areca palm yellow leaf disease . | SCIENCE BULLETIN , 2025 , 70 (6) , 847-851 . |
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Viral pathogens not only threaten the health and life of humans and animals but also cause enormous crop yield losses and contribute to global food insecurity. To defend against viral pathogens, plants have evolved an intricate immune system to perceive and cope with such attacks. Although most of the fundamental studies were carried out in model plants, more recent research in crops has provided new insights into the antiviral strategies employed by crop plants. We summarize recent advances in understanding the biological roles of cellular receptors, RNA silencing, RNA decay, hormone signaling, autophagy, and ubiquitination in manipulating crop host-mediated antiviral responses. The potential functions of circular RNAs, the rhizosphere microbiome, and the foliar microbiome of crops in plant-virus interactions will be fascinating research directions in the future. These findings will be beneficial for the development of modern crop improvement strategies.
Keyword ：
antiviral defense antiviral defense autophagy autophagy crops crops RNA silencing RNA silencing ubiquitin proteasome system ubiquitin proteasome system
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| GB/T 7714 | Yang, Zhirui , Li, Guangyao , Zhang, Yongliang et al. Crop antiviral defense: Past and future perspective [J]. | SCIENCE CHINA-LIFE SCIENCES , 2024 , 67 (12) : 2617-2634 . |
| MLA | Yang, Zhirui et al. "Crop antiviral defense: Past and future perspective" . | SCIENCE CHINA-LIFE SCIENCES 67 . 12 (2024) : 2617-2634 . |
| APA | Yang, Zhirui , Li, Guangyao , Zhang, Yongliang , Li, Fangfang , Zhou, Tao , Ye, Jian et al. Crop antiviral defense: Past and future perspective . | SCIENCE CHINA-LIFE SCIENCES , 2024 , 67 (12) , 2617-2634 . |
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Plant viruses are a group of intracellular pathogens that persistently threaten global food security. Significant advances in plant virology have been achieved by Chinese scientists over the last 20 years, including basic research and technologies for preventing and controlling plant viral diseases. Here, we review these milestones and advances, including the identification of new crop-infecting viruses, dissection of pathogenic mechanisms of multiple viruses, examination of multilayered interactions among viruses, their host plants, and virus-transmitting arthropod vectors, and in-depth interrogation of plant-encoded resistance and susceptibility determinants. Notably, various plant virus-based vectors have also been successfully developed for gene function studies and target gene expression in plants. We also recommend future plant virology studies in China.
Keyword ：
China China emerging plant viruses emerging plant viruses plant resistance/susceptibility to viral infection plant resistance/susceptibility to viral infection plant virology plant virology plant virus-based vectors plant virus-based vectors plant-virus transmission vector interactions plant-virus transmission vector interactions viral pathogenesis viral pathogenesis
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| GB/T 7714 | Wu, Jianguo , Zhang, Yongliang , Li, Fangfang et al. Plant virology in the 21st century in China: Recent advances and future directions [J]. | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2024 , 66 (3) : 579-622 . |
| MLA | Wu, Jianguo et al. "Plant virology in the 21st century in China: Recent advances and future directions" . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY 66 . 3 (2024) : 579-622 . |
| APA | Wu, Jianguo , Zhang, Yongliang , Li, Fangfang , Zhang, Xiaoming , Ye, Jian , Wei, Taiyun et al. Plant virology in the 21st century in China: Recent advances and future directions . | JOURNAL OF INTEGRATIVE PLANT BIOLOGY , 2024 , 66 (3) , 579-622 . |
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Ophiocordyceps sinensis appears as stroma emerging from underground sclerotium enclosed by the skeleton of Thitarodes moth larvae. However, the actual distribution of the fungus in soil still remains unclarified. In this study, 40 soil samples were used for detection of O. sinensis to confirm its distribution in native habitats using denaturing gradient gel electrophoresis, nested internal transcribed spacer (ITS) PCR, and 454 pyrosequencing methods. The soil samples included six types: Os, where both stromata and host moth larvae were found; NL, representing no signs of stromata, but where moth larvae were found; NOs, where neither stroma nor moth larvae were found; BS, with bare soil without the presence of stroma of O. sinensis or moth larvae; AF, from soil surrounding the stroma; and MP, soil particles firmly wrapping the sclerotium of O. sinensis. Of 40 samples tested, 36 showed positive detection of O. sinensis by at least one of the three detection methods, with positive detection in all six sample types at all five sites. The results showed that traces of O. sinensis can be detected in locations with no macroscopically visible evidence of the fungus or its host and at least 100 m away from such locations.
Keyword ：
DGGE DGGE distribution distribution medicinal mushrooms medicinal mushrooms nested PCR nested PCR Ophiocordyceps sinensis Ophiocordyceps sinensis pyrosequencing pyrosequencing
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| GB/T 7714 | Yang, Rui-Heng , Wang, Xiao-Liang , Wang, Ke et al. Deep Sequencing, Nested PCR, and Denaturing Gradient Gel Electrophoresis Reveal a Wider Distribution of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), in Native Soil Types [J]. | INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS , 2021 , 23 (4) : 93-104 . |
| MLA | Yang, Rui-Heng et al. "Deep Sequencing, Nested PCR, and Denaturing Gradient Gel Electrophoresis Reveal a Wider Distribution of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), in Native Soil Types" . | INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS 23 . 4 (2021) : 93-104 . |
| APA | Yang, Rui-Heng , Wang, Xiao-Liang , Wang, Ke , Hsiang, Tom , Wang, Wen-Jing , Wu, Hai-Jun et al. Deep Sequencing, Nested PCR, and Denaturing Gradient Gel Electrophoresis Reveal a Wider Distribution of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), in Native Soil Types . | INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS , 2021 , 23 (4) , 93-104 . |
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Kenaf (Hibiscus cannabinus L.) is an important bast fiber crop, and a source of fiber to many industries. This crop is diploid (2n = 2x = 36) in nature, and belongs to Malvaceae family. Kenaf breeding is directed to develop the new cultivars that are better in fiber production, fiber quality, resistant to anthracnose, photo-insensitivity, resistant to salt and drought stresses, and so on. This review describes the various successes in kenaf breeding, and reports the future opportunities. The various classical and advanced breeding approaches have great potential to achieve the goals. However, the recent approaches in plant breeding and biotechnology could have more prospective to accelerate the kenaf breeding. These may include genomic selection, CRISPR/Cas9 gene-editing system, and other omics-based approaches. We suggest that the integration of recent approaches with classical ones has vital importance in improving the efficiency, speed, and accuracy of kenaf breeding.
Keyword ：
Bast fiber Bast fiber biotechnology biotechnology genetic diversity genetic diversity heterosis utilization heterosis utilization kenaf kenaf molecular markers molecular markers
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| GB/T 7714 | Afzal, Muhammad Zohaib , Ibrahim, Aminu Kurawa , Xu, Yi et al. Kenaf (Hibiscus Cannabinus L.) Breeding [J]. | JOURNAL OF NATURAL FIBERS , 2020 , 19 (11) : 4063-4081 . |
| MLA | Afzal, Muhammad Zohaib et al. "Kenaf (Hibiscus Cannabinus L.) Breeding" . | JOURNAL OF NATURAL FIBERS 19 . 11 (2020) : 4063-4081 . |
| APA | Afzal, Muhammad Zohaib , Ibrahim, Aminu Kurawa , Xu, Yi , Niyitanga, Sylvain , Li, Yi , Li, Dongxu et al. Kenaf (Hibiscus Cannabinus L.) Breeding . | JOURNAL OF NATURAL FIBERS , 2020 , 19 (11) , 4063-4081 . |
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Megasporogenesis is a key step during ovule development in angiosperms, but the small number and inaccessibility of these cells have hampered molecular and genome-wide studies. Thus, many questions remain regarding the molecular basis of cell specification, differentiation, and development in the female gametophyte. Here, taking advantage of the correlation between spikelet length and ovule development in rice (Oryza sativa), we studied the transcriptome dynamics of young ovules at three stages, the archesporial cell, the megaspore mother cell before meiosis, and the functional megaspore after meiosis, using expression profiling based on RNA sequencing. Our analysis showed that 5,274 genes were preferentially expressed in ovules during megasporogenesis as compared to ovules at the mature female gametophyte stage. Out of these, 958 (18.16%) genes were archesporial cell- and/or megaspore mother cell-preferential genes, and represent a significant enrichment of genes involved in hormone signal transduction and plant pathogen interaction pathways, as well as genes encoding transcription factors. The expression patterns of nine genes that were preferentially expressed in ovules of different developmental stages, including the OsERECTA2 (OsER2) receptor-like kinase gene, were confirmed by in situ hybridization. We further characterized the OsER2 loss-of-function mutant, which had an excessive number of female germline cells and an abnormal female gametophyte, suggesting that OsER2 regulates germline cell specification during megasporogenesis in rice. These results expand our understanding of the molecular control of megasporogenesis in rice and contribute to the functional studies of genes involved in megasporogenesis. Transcriptome analysis of young ovules points to a receptor-like kinase that is preferentially expressed in archesporial and megaspore mother cells and functions in female germline cell specification in rice.
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| GB/T 7714 | Zhao, Heming , Guo, Mingliang , Yan, Maokai et al. Comparative Expression Profiling Reveals Genes Involved in Megasporogenesis1 [J]. | PLANT PHYSIOLOGY , 2020 , 182 (4) : 2006-2024 . |
| MLA | Zhao, Heming et al. "Comparative Expression Profiling Reveals Genes Involved in Megasporogenesis1" . | PLANT PHYSIOLOGY 182 . 4 (2020) : 2006-2024 . |
| APA | Zhao, Heming , Guo, Mingliang , Yan, Maokai , Cheng, Han , Liu, Yanhui , She, Zeyuan et al. Comparative Expression Profiling Reveals Genes Involved in Megasporogenesis1 . | PLANT PHYSIOLOGY , 2020 , 182 (4) , 2006-2024 . |
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