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学者姓名：李亚
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Abstract ：
Unique genes refer to genes specific to a particular organism and play crucial roles in the biological functions, evolutionary processes, and adaptations to external environments. However, the roles of unique genes in plant pathogenic fungi remain largely unexplored. In this study, we identified a novel unique gene in the rice blast fungus Magnaporthe oryzae, named MoUNG (M. oryzae unique gene), through T-DNA insertion mutagenesis. The disruption of the MoUNG promoter region in the T-DNA insertion mutant (T30-104) led to an almost loss of MoUNG expression. MoUNG has no functional domains and lacks homologues in other organism. It is highly expressed during the early-infection stage between 16 and 32 h post-inoculation (HPI), in contrast to its expression in mycelia and at the later infection stage of 48 HPI. Notably, attempts to knock out MoUNG were unsuccessful, so we examined the T30-104 mutant and found it showed significantly reduced growth, conidiation, and pathogenicity. Introducing the full-length MoUNG with its promoter into T30-104 restored these phenotypic defects. Additionally, subcellular localization assays revealed that MoUNG exhibits a dot-like distribution within the cytoplasm of mycelium, conidium, appressorium, and invasive hypha. Furthermore, knock-down of MoUNG produced results similar to those observed with the insertion mutation. In conclusion, we identified a novel unique gene MoUNG in M. oryzae and demonstrated its involvement in growth, conidiation, and pathogenicity.
Keyword ：
functional characterization functional characterization Magnaporthe oryzae Magnaporthe oryzae unique gene MoUNG unique gene MoUNG
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| GB/T 7714 | Chen, Jing , He, Qingfeng , Xie, Xuze et al. The Identification of a Unique Gene MoUNG Required for Growth, Conidiation, and Pathogenicity in Magnaporthe oryzae Through T-DNA Insertion Mutagenesis [J]. | AGRONOMY-BASEL , 2025 , 15 (2) . |
| MLA | Chen, Jing et al. "The Identification of a Unique Gene MoUNG Required for Growth, Conidiation, and Pathogenicity in Magnaporthe oryzae Through T-DNA Insertion Mutagenesis" . | AGRONOMY-BASEL 15 . 2 (2025) . |
| APA | Chen, Jing , He, Qingfeng , Xie, Xuze , Wu, Yuting , Liu, Shan , Li, Xihong et al. The Identification of a Unique Gene MoUNG Required for Growth, Conidiation, and Pathogenicity in Magnaporthe oryzae Through T-DNA Insertion Mutagenesis . | AGRONOMY-BASEL , 2025 , 15 (2) . |
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Mad2, a conserved core component of the spindle assembly checkpoint (SAC) in eukaryotes, delays anaphase onset in case of incorrect kinetochore-microtubule attachment. However, its functions in plant-pathogenic fungi remain largely unknown. Here, we identified the Mad2 homologue in rice blast fungus Magnaporthe oryzae (MoMad2), which shows high similarity with Mad2 in fission yeast. When expressed in fission yeast, MoMad2 associated with native SpMad1 and SpCdc20, and successfully rescued the Delta Spmad2 mutant's defect in arresting anaphase onset upon damaged spindle, indicating the conserved SAC function of MoMad2. Moreover, MoMad2 interacted with MoMad1 and depends on MoMad1 for its nuclear envelope-localisation. Although it plays a dispensable role in M. oryzae growth, MoMad2 is required for tolerance to the microtubule depolymerising agent treatment. Delta Momad2 mutants exhibited shorter hyphal compartments and earlier conidial germination and appressorium formation, suggesting that MoMad2 deletion shortens M. oryzae's mitotic cell cycle due to defective SAC arrest. Additionally, knockout of MoMAD2 decreased the appressorial turgor pressure, impaired appressorium penetration and compromised M. oryzae pathogenicity. Taken together, our findings revealed that MoMad2, as a conserved component in SAC signalling, is essential for full pathogenicity of rice blast fungus.
Keyword ：
appressorial turgor appressorial turgor Mad2 Mad2 Magnaporthe oryzae Magnaporthe oryzae pathogenicity pathogenicity spindle assembly checkpoint spindle assembly checkpoint
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| GB/T 7714 | Shen, Tianjiao , Chen, Qiushi , Leontiou, Ioanna et al. MoMad2 With a Conserved Function in the Spindle Assembly Checkpoint Is Required for Maintaining Appressorial Turgor Pressure and Pathogenicity of Rice Blast Fungus [J]. | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (9) . |
| MLA | Shen, Tianjiao et al. "MoMad2 With a Conserved Function in the Spindle Assembly Checkpoint Is Required for Maintaining Appressorial Turgor Pressure and Pathogenicity of Rice Blast Fungus" . | MOLECULAR PLANT PATHOLOGY 26 . 9 (2025) . |
| APA | Shen, Tianjiao , Chen, Qiushi , Leontiou, Ioanna , Wang, Rong , Su, Meiling , Luo, Qiong et al. MoMad2 With a Conserved Function in the Spindle Assembly Checkpoint Is Required for Maintaining Appressorial Turgor Pressure and Pathogenicity of Rice Blast Fungus . | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (9) . |
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Plant lectins have a significant impact on the defense against pathogens and insect attacks. The jacalin-related lectin OsMbl1 from rice (Oryza sativa L.) has been reported to play a crucial role in pattern-triggered immunity (PTI). However, the underlying mechanism remains unclear. In this study, we identified a GDSL-like lipase, OsGdsl1, that interacts with OsMbl1 both in vitro and in vivo. The OsGdsl1 protein, which has lipase activity, is localized in the lipid bodies and apoplast. The expression of OsGDSL1 is modulated upon exposure to Magnaporthe oryzae (M. oryzae) or plant hormones. Deletion of the OsGDSL1 gene not only improved the resistance of rice to M. oryzae, but also led to an increased ROS burst after chitin treatments. The expression of some pathogenesis-related (PR) genes was upregulated in the mutants. We also found that OsMbl1 inhibited the lipase activity of OsGdsl1 during infection with M. oryzae. Overall, our results suggest that OsGdsl1 negatively regulates rice immunity to M. oryzae infection by downregulating ROS bursts and PR gene expressions, while its lipase activity, which is inhibited by OsMbl1, contributes to the enhancement of rice innate immunity during M. oryzae infection.
Keyword ：
lipase lipase Magnaporthe oryzae Magnaporthe oryzae OsMbl1 OsMbl1 plant immunity plant immunity rice rice
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| GB/T 7714 | Song, Linlin , Yang, Tao , Abubakar, Yakubu Saddeeq et al. OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) : 5650-5663 . |
| MLA | Song, Linlin et al. "OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity" . | PLANT CELL AND ENVIRONMENT 48 . 8 (2025) : 5650-5663 . |
| APA | Song, Linlin , Yang, Tao , Abubakar, Yakubu Saddeeq , Han, Yijuan , Zhang, Ruina , Li, Ya et al. OsMbl1 Counteracts OsGdsl1-Mediated Rice Blast Susceptibility by Inhibiting Its Lipase Activity . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) , 5650-5663 . |
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Keyword ：
Annona squamosa Annona squamosa China China CO3 gene CO3 gene LSU rDNA LSU rDNA Phakopsora cherimoliae Phakopsora cherimoliae rust disease rust disease
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| GB/T 7714 | Huang, Qi , Wan, Jing , Li, Xihong et al. First Report of Phakopsora cherimoliae Causing Rust Disease on Annona squamosa in China [J]. | PLANT DISEASE , 2025 , 109 (11) . |
| MLA | Huang, Qi et al. "First Report of Phakopsora cherimoliae Causing Rust Disease on Annona squamosa in China" . | PLANT DISEASE 109 . 11 (2025) . |
| APA | Huang, Qi , Wan, Jing , Li, Xihong , Zhao, Shengmei , Liu, Guokun , Li, Ya et al. First Report of Phakopsora cherimoliae Causing Rust Disease on Annona squamosa in China . | PLANT DISEASE , 2025 , 109 (11) . |
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Rice blast disease, caused by the fungus Magnaporthe oryzae, is a significant threat to rice production. Resistant cultivars can effectively resist the invasion of M. oryzae. Thus, the identification of disease-resistant genes is of utmost importance for improving rice production. Autophagy, a cellular process that recycles damaged components, plays a vital role in plant growth, development, senescence, stress response, and immunity. To understand the involvement of autophagy-related genes (ATGs) in rice immune response against M. oryzae, we conducted a comprehensive analysis of 37 OsATGs, including bioinformatic analysis, transcriptome analysis, disease resistance analysis, and protein interaction analysis. Bioinformatic analysis revealed that the promoter regions of 33 OsATGs contained cis-acting elements responsive to salicylic acid (SA) or jasmonic acid (JA), two key hormones involved in plant defense responses. Transcriptome data showed that 21 OsATGs were upregulated during M. oryzae infection. Loss-of-function experiments demonstrated that OsATG6c, OsATG8a, OsATG9b, and OsATG13a contribute to rice blast resistance. Additionally, through protein interaction analysis, we identified five proteins that may interact with OsATG13a and potentially contribute to plant immunity. Our study highlights the important role of autophagy in rice immunity and suggests that OsATGs may enhance resistance to rice blast fungus through the involvement of SA, JA, or immune-related proteins. These findings provide valuable insights for future efforts in improving rice production through the identification and utilization of autophagy-related genes.
Keyword ：
autophagy autophagy jasmonic acid jasmonic acid Magnaporthe oryzae Magnaporthe oryzae OsATGs OsATGs plant immunity plant immunity salicylic acid salicylic acid
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| GB/T 7714 | Xie, Xuze , Pei, Mengtian , Liu, Shan et al. Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against Magnaporthe oryzae [J]. | PLANTS-BASEL , 2024 , 13 (7) . |
| MLA | Xie, Xuze et al. "Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against Magnaporthe oryzae" . | PLANTS-BASEL 13 . 7 (2024) . |
| APA | Xie, Xuze , Pei, Mengtian , Liu, Shan , Wang, Xinxiao , Gong, Shanshan , Chen, Jing et al. Comprehensive Analysis of Autophagy-Related Genes in Rice Immunity against Magnaporthe oryzae . | PLANTS-BASEL , 2024 , 13 (7) . |
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The spindle assembly checkpoint (SAC) proteins are conserved among eukaryotes safeguarding chromosome segregation fidelity during mitosis. However, their biological functions in plant-pathogenic fungi remain largely unknown. In this study, we found that the SAC protein MoMad1 in rice blast fungus (Magnaporthe oryzae) localizes on the nuclear envelope and is dispensable for M. oryzae vegetative growth and tolerance to microtubule depolymerizing agent treatment. MoMad1 plays an important role in M. oryzae infection-related development and pathogenicity. The monopolar spindle 1 homologue in M. oryzae (MoMps1) interacts with MoMad1 through its N-terminal domain and phosphorylates MoMad1 at Ser-18, which is conserved within the extended N termini of Mad1s from fungal plant pathogens. This phosphorylation is required for maintaining MoMad1 protein abundance and M. oryzae full virulence. Similar to the deletion of MoMad1, treatment with Mps1-IN-1 (an Mps1 inhibitor) caused compromised appressorium formation and decreased M. oryzae virulence, and these defects were dependent on its attenuating MoMad1 Ser-18 phosphorylation. Therefore, our study indicates the function of Mad1 in rice blast fungal pathogenicity and sheds light on the potential of blocking Mad1 phosphorylation by Mps1 to control crop fungal diseases.
Keyword ：
Mad1 Mad1 Mps1 Mps1 pathogenicity pathogenicity phosphorylation phosphorylation rice blast fungus rice blast fungus
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| GB/T 7714 | Chen, Qiushi , Li, Ya , Shen, Tianjiao et al. Phosphorylation of Mad1 at serine 18 by Mps1 is required for the full virulence of rice blast fungus, Magnaporthe oryzae [J]. | MOLECULAR PLANT PATHOLOGY , 2024 , 25 (4) . |
| MLA | Chen, Qiushi et al. "Phosphorylation of Mad1 at serine 18 by Mps1 is required for the full virulence of rice blast fungus, Magnaporthe oryzae" . | MOLECULAR PLANT PATHOLOGY 25 . 4 (2024) . |
| APA | Chen, Qiushi , Li, Ya , Shen, Tianjiao , Wang, Rong , Su, Meiling , Luo, Qiong et al. Phosphorylation of Mad1 at serine 18 by Mps1 is required for the full virulence of rice blast fungus, Magnaporthe oryzae . | MOLECULAR PLANT PATHOLOGY , 2024 , 25 (4) . |
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Isw2 proteins, ubiquitous across eukaryotes, exhibit a propensity for DNA binding and exert dynamic influences on local chromosome condensation in an ATP-dependent fashion, thereby modulating the accessibility of neighboring genes to transcriptional machinery. Here, we report the deletion of a putative MoISW2 gene, yielding substantial ramifications on plant pathogenicity. Subsequent gene complementation and chromatin immunoprecipitation sequencing (ChIP-seq) analyses were conducted to delineate binding sites. RNA sequencing (RNA-seq) assays revealed discernible impacts on global gene regulation along chromosomes in both mutant and wild-type strains, with comparative analyses against 55 external RNA-seq data sets corroborating these findings. Notably, MoIsw2-mediated binding and activities delineate genomic loci characterized by pronounced gene expression variability proximal to MoIsw2 binding sites, juxtaposed with comparatively stable expression in surrounding regions. The contingent genes influenced by MoIsw2 activity predominantly encompass niche-determinant genes, including those encoding secreted proteins, secondary metabolites, and stress-responsive elements, alongside avirulence genes. Furthermore, our investigations unveil a spatial correlation between MoIsw2 binding motifs and known transposable elements (TEs), suggesting a potential interplay wherein TE transposition at these loci could modulate the transcriptional landscape of Magnaporthe oryzae in a strain-specific manner. Collectively, these findings position MoIsw2 as a plausible master regulator orchestrating the delicate equilibrium between genes vital for biomass proliferation, akin to housekeeping genes, and niche-specific determinants crucial for ecological adaptability. Stress-induced TE transposition, in conjunction with MoIsw2 activity, emerges as a putative mechanism fostering enhanced mutagenesis and accelerated evolution of niche-determinant genes relative to housekeeping counterparts.
Keyword ：
avirulence genes avirulence genes epigenetics epigenetics evolution evolution heterochromatin heterochromatin nucleosomes nucleosomes retrotransposons retrotransposons
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| GB/T 7714 | Pei, Mengtian , Abubakar, Yakubu Saddeeq , Ali, Hina et al. Whole genome regulatory effect of MoISW2 and consequences for the evolution of the rice plant pathogenic fungus Magnaporthe oryzae [J]. | MBIO , 2024 , 15 (10) . |
| MLA | Pei, Mengtian et al. "Whole genome regulatory effect of MoISW2 and consequences for the evolution of the rice plant pathogenic fungus Magnaporthe oryzae" . | MBIO 15 . 10 (2024) . |
| APA | Pei, Mengtian , Abubakar, Yakubu Saddeeq , Ali, Hina , Lin, Lianyu , Dou, Xianying , Lu, Guodong et al. Whole genome regulatory effect of MoISW2 and consequences for the evolution of the rice plant pathogenic fungus Magnaporthe oryzae . | MBIO , 2024 , 15 (10) . |
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The Myb family of transcription factors (TFs) is a large and functionally diverse group found in all eukaryotes. Its role in fungi remains poorly studied, despite the fact that it is thought to play a role in the pathogenicity of fungal pathogens. In this study, we have characterized the functional role of a Myb family TF called MoMyb13 in the rice blast fungus, Magnaporthe oryzae. MoMyb13 has orthologues only in ascomycete fungi, making it of special interest. Localization experiments confirmed that MoMyb13 is located in the nuclei, as expected for a TF. Phenotypic analysis showed that MoMyb13 mutants exhibited reduced growth, white instead of dark colonies, formed no conidia and, consequently, no conidial appressoria. The mutants completely lost pathogenicity, despite being able to form dark hyphal appressoria at their hyphae ends. Furthermore, the mutant colonies lost hydrophobicity and had significantly reduced expression of the hydrophobin MPG1 that MoMyb13 appears to regulate. However, overexpression of MPG1 in the mutants restored hydrophobicity, but not pathogenicity. Stress assay showed that the mutants were more sensitive to SDS, CR, and H2O2, but more tolerant to NaCl and SOR. In summary, our study revealed the crucial function of MoMyb13 in the growth, conidiation, hydrophobicity, stress response, and pathogenicity of M. oryzae. MoMyb13 is thus needed in the late and very early stages of infection for the spreading of the fungus to other plants and the early establishment of infection in other plants.
Keyword ：
functional characterization functional characterization Magnaporthe oryzae Magnaporthe oryzae Myb Myb transcription factor transcription factor
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| GB/T 7714 | Li, Ya , Zheng, Xiuxia , Pei, Mengtian et al. Functional Analysis of MoMyb13, a Myb Transcription Factor Involved in Regulating Growth, Conidiation, Hydrophobicity, and Pathogenicity of Magnaporthe oryzae [J]. | AGRONOMY-BASEL , 2024 , 14 (2) . |
| MLA | Li, Ya et al. "Functional Analysis of MoMyb13, a Myb Transcription Factor Involved in Regulating Growth, Conidiation, Hydrophobicity, and Pathogenicity of Magnaporthe oryzae" . | AGRONOMY-BASEL 14 . 2 (2024) . |
| APA | Li, Ya , Zheng, Xiuxia , Pei, Mengtian , Chen, Mengting , Zhang, Shengnan , Liang, Chenyu et al. Functional Analysis of MoMyb13, a Myb Transcription Factor Involved in Regulating Growth, Conidiation, Hydrophobicity, and Pathogenicity of Magnaporthe oryzae . | AGRONOMY-BASEL , 2024 , 14 (2) . |
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The conserved DNA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and plants.However,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains unknown.In this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in M.oryzae.We first demonstrated the importance of DNA damage repair in M.oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this fungus.Additionally,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in M.oryzae.Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M.oryzae,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DNA damage repair.Overall,our results provide insights into the roles of MoMMS21 and MoSMC5 in M.oryzae,highlighting their functions beyond DNA damage repair.
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| GB/T 7714 | Yue Jiang , Rong Wang , Lili Du et al. The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae [J]. | 农业科学学报（英文版） , 2024 , 23 (6) : 1956-1966 . |
| MLA | Yue Jiang et al. "The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae" . | 农业科学学报（英文版） 23 . 6 (2024) : 1956-1966 . |
| APA | Yue Jiang , Rong Wang , Lili Du , Xueyu Wang , Xi Zhang , Pengfei Qi et al. The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae . | 农业科学学报（英文版） , 2024 , 23 (6) , 1956-1966 . |
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【目的】MYB(V-myb avian myeloblastosis viral oncogene homolog)转录因子MoIsw2对稻瘟病菌的生长发育和致病性至关重要。为了深入理解MoIsw2对稻瘟病菌的作用机制，对其调控的基因进行鉴定和功能分析。【方法】对野生型稻瘟病菌菌株Ku80和突变体ΔMoisw2进行RNA-seq分析，选择突变体中表达发生明显变化的7个基因(MoEGH16L1、MoEGH16L2、MoSTART1、MoGH61A、MoCTR1、MoFRO1和MoGH31A)作为MoIsw2的候选调控基因，通过基因敲除对其表型和致病性进行分析。【结果】RNA-seq分析表明，突变体ΔMoisw2中差异表达的基因主要涉及氧化还原、细胞膜合成、氨基酸代谢和基因组DNA修复等过程。7个候选调控基因的敲除试验结果表明，这些基因在稻瘟病菌的营养生长、孢子发育和致病等过程中发挥重要作用，各基因突变体表型与突变体ΔMoisw2均具有相似之处。【结论】MoIsw2通过调控多个靶基因参与稻瘟病菌的生长发育和致病过程。
Keyword ：
Isw2 Isw2 MYB转录因子 MYB转录因子 基因功能 基因功能 稻瘟病菌 稻瘟病菌 转录组测序 转录组测序
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| GB/T 7714 | 裴梦甜 , 王鑫潇 , 余庆 et al. 稻瘟病菌MYB转录因子MoIsw2调控基因的鉴定和功能分析 [J]. | 福建农林大学学报(自然科学版) , 2024 , 53 (05) : 598-609 . |
| MLA | 裴梦甜 et al. "稻瘟病菌MYB转录因子MoIsw2调控基因的鉴定和功能分析" . | 福建农林大学学报(自然科学版) 53 . 05 (2024) : 598-609 . |
| APA | 裴梦甜 , 王鑫潇 , 余庆 , 鲁国东 , 李亚 . 稻瘟病菌MYB转录因子MoIsw2调控基因的鉴定和功能分析 . | 福建农林大学学报(自然科学版) , 2024 , 53 (05) , 598-609 . |
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