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学者姓名：王宗文
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Abstract ：
The growth of gold nanobipyramids (AuNBPs) provides a superior colorimetric quantification method for point-of-care testing due to their superior growth uniformity, high-resolution multicolor response, and excellent seed storage stability. However, conventional growth methods face significant limitations in sensing applications due to their reliance on a single reductant and stringent acidic conditions. This study systematically investigated the reductant mechanism in AuNBP growth, revealing that the pH-regulated redox potential is crucial for achieving anisotropic AuNBP growth. By employing nine different reductants, the growth pH was expanded from 1.5 to 8.5. Notably, dihydroxybenzene-mediated systems exhibited a slender morphology, reduced reductant consumption, enhanced stability, and comparable analytical performance compared to the conventional growth system. Among these systems, the hydroquinone (HQ)-mediated system was integrated with horseradish peroxidase (HRP)-catalyzed reactions in a pH-compatible manner. By incorporating this integrated system into a commercial HRP immunoassay, a novel multicolor immunosensor was developed for thyroglobulin (Tg) detection. This immunosensor exhibited a distinct nine-color gradient across a ten-fold Tg concentration range (0.1-1.3 ng/mL), achieving a visual detection limit of 0.1 ng/mL and an instrumental detection limit of 29 pg/mL. It accurately detected Tg in human serum with good recoveries of 94.1 %-104.8 % and signal stability for six hours. The results correlated well with the commercial immunoassay while offering superior visual readout for point-of-care settings. These findings validate the applicability of the HQ-mediated system in converting conventional single-color HRP immunoassays to multicolor immunosensors. Furthermore, expanding reductant selection and pH adaptability in AuNBP growth offers more possibilities for developing versatile pH-flexible multicolor sensors.
Keyword ：
Gold disproportionation Gold disproportionation Gold nanobipyramids Gold nanobipyramids Growth method Growth method Multicolor immunosensor Multicolor immunosensor Thyroglobulin Thyroglobulin
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| GB/T 7714 | Xu, Quanming , Zhang, Feng , Luo, Guizhen et al. Generalizable multicolor sensing platform derived from horseradish peroxidase immunoassays via broad-pH-tunable growth of gold nanobipyramids: Application in thyroglobulin detection [J]. | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 446 . |
| MLA | Xu, Quanming et al. "Generalizable multicolor sensing platform derived from horseradish peroxidase immunoassays via broad-pH-tunable growth of gold nanobipyramids: Application in thyroglobulin detection" . | SENSORS AND ACTUATORS B-CHEMICAL 446 (2025) . |
| APA | Xu, Quanming , Zhang, Feng , Luo, Guizhen , Liu, Jiayi , He, Xue , Wang, Zemiao et al. Generalizable multicolor sensing platform derived from horseradish peroxidase immunoassays via broad-pH-tunable growth of gold nanobipyramids: Application in thyroglobulin detection . | SENSORS AND ACTUATORS B-CHEMICAL , 2025 , 446 . |
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Monilinia fructicola is one of main pathogens causing brown rot in peach. Fungicide applications are the most practical way to manage this disease still now, however, overuse of fungicide has led to pathogen resistance. In this study, the susceptibility of 87 field strains of M. fructicola to iprodione was investigated. The results showed that two of them were resistant confirmed by fruit assay of iprodione application. Further investigation showed higher osmotic sensitivity in the resistant strains. Therefore, the four whole osmosensing two-component histidine kinase genes (MfOs-1, MfOs-2, MfOs-4 and MfOs-5) were amplified and the corresponding amino acid sequences comparison showed that two-point mutations in MfOs-1 associated with iprodione resistance. One of the two mutations is with alanine replaced by valine at 307 position (A307V) and another is glycine replaced by serine at 462 position (G462S). Further fitness parameters investigation showed penalty in resistant strains indicating by reduced mycelial growth rate, less sporulation, and smaller lesion size on detached fruit. This is the first report of M. fructicola field strains resistant to dicarboximide fungicides. The results obtained in this study will provide a basis for effectively control the spread of brown rot.
Keyword ：
DCF fungicide DCF fungicide Fungicide resistance Fungicide resistance Monilinia fructicola Monilinia fructicola Os-1 Os-1 Point mutation Point mutation
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| GB/T 7714 | Long, Qian , Guo, Yiyi , Yi, Miaomiao et al. New point mutations in osmosensing two-component histidine kinase gene associated with iprodione resistance in Monilinia fructicola field isolates from China [J]. | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 213 . |
| MLA | Long, Qian et al. "New point mutations in osmosensing two-component histidine kinase gene associated with iprodione resistance in Monilinia fructicola field isolates from China" . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 213 (2025) . |
| APA | Long, Qian , Guo, Yiyi , Yi, Miaomiao , Lu, Changsheng , Zhong, Rui , Zhang, Hao et al. New point mutations in osmosensing two-component histidine kinase gene associated with iprodione resistance in Monilinia fructicola field isolates from China . | PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY , 2025 , 213 . |
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The infection and resistance of pathogenic fungi pose a significant threat to food crop safety, highlighting the urgent need for new green antifungal agents. Fusarium acid (FA) with its simple and novel structure correlates with the principles of green pesticides and has demonstrated substantial broad-spectrum antifungal activity. In a previous study, several promising lead compounds were identified. This study focused on further optimizing lead compound A1. Its inhibitory effect was evaluated against six different pathogenic fungi and several new promising compounds were identified. Notably, the anti-Magnaporthe oryzae (M. oryzae) activity of compound B4 was significantly enhanced with an EC50 value of 910 ng/mL. Rice safety tests indicated that B4 not only exhibited no adverse effect on rice growth and chlorophyll synthesis but also induced plant immunity by increasing the levels of polyphenol oxidase (PPO), peroxidase (POD), and superoxide dismutase (SOD), and soluble sugars. Further investigations into the antifungal mechanism of B4 indicated that it enhanced the permeability and damage of the mycelial membrane, thereby leading to electrolyte leakage and the loss of essential cellular substances. Subsequently, B4 induced the production of reactive oxygen species (ROS) in M. oryzae cell, including center dot OH, center dot O2 -, and 1O2, while promoting increased levels of malondialdehyde (MDA) and SOD enzyme activity. Rice infection tests indicated that B4 effectively inhibited M. oryzae conidia activity and inhibited melanin production. These findings indicated that FA derivatives hold significant potential as a new strategy for M. oryzae disease management and offer valuable insights for the control of plant fungal diseases.
Keyword ：
3D-QSAR 3D-QSAR fungicidal activity fungicidal activity fusaric acid derivatives fusaric acid derivatives safety index safety index
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| GB/T 7714 | Huang, Bin Bin , Gao, Ming Wei , Wang, Xi Guang et al. From Spear to Shield: A Novel Antifungal Drug that Safely Enhances Rice Immune Defenses [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (13) : 7547-7561 . |
| MLA | Huang, Bin Bin et al. "From Spear to Shield: A Novel Antifungal Drug that Safely Enhances Rice Immune Defenses" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 13 (2025) : 7547-7561 . |
| APA | Huang, Bin Bin , Gao, Ming Wei , Wang, Xi Guang , Huang, Xin Yi , Li, Guo , Song, Li Yan et al. From Spear to Shield: A Novel Antifungal Drug that Safely Enhances Rice Immune Defenses . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (13) , 7547-7561 . |
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The development of simple, sensitive, and specific colorimetric methods for screening fentanyl (FTN) and its analogs is critically important for public health surveillance and drug control efforts. This study presents a novel multicolor colorimetric aptasensor based on gold nanobipyramid (AuNBP) growth for FTN and acetylfentanyl (AFTN) detection. First, a magnetic bead separation-assisted competitive binding system was developed using a high-affinity FTN aptamer (F27), which was demonstrated to specifically bind to FTN and AFTN, forming a parallel G-quadruplex structure. Then, we demonstrated that hydroquinone (HQ) can regulate AuNBP growth. This growth method is pH-compatible with a horseradish peroxidase (HRP) enzymatic reaction, leading to the development of a novel growth system termed HRP-catalyzed HQ-regulated AuNBP growth. By integrating these two systems, the developed aptasensor demonstrated similar signal responses and eight color changes for the detection of FTN and AFTN. The visual detection limit was 10 nM for both FTN and AFTN, and instrumental detection limits were 6.4 nM for FTN and 6.9 nM for AFTN. The aptasensor exhibited excellent sensitivity, specificity, and resistance to interference, and was successfully used to analyze spiked saliva and urine samples with a recovery of 95.9 %-102.9 % and an RSD (n = 5) < 5 %, making it a promising method for visual on-site detection of total FTN and AFTN concentration in human saliva and urine samples.
Keyword ：
Acetylfentanyl Acetylfentanyl Body fluids Body fluids Fentanyl Fentanyl Gold nanobipyramid growth Gold nanobipyramid growth Multicolor aptasensor Multicolor aptasensor Visual detection Visual detection
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| GB/T 7714 | Xu, Quanming , He, Xue , Luo, Guizhen et al. A sensitive multicolor colorimetric aptasensor for on-site detection of total fentanyl and acetylfentanyl concentration based on horseradish peroxidase-regulated gold nanobipyramid growth [J]. | MICROCHEMICAL JOURNAL , 2025 , 219 . |
| MLA | Xu, Quanming et al. "A sensitive multicolor colorimetric aptasensor for on-site detection of total fentanyl and acetylfentanyl concentration based on horseradish peroxidase-regulated gold nanobipyramid growth" . | MICROCHEMICAL JOURNAL 219 (2025) . |
| APA | Xu, Quanming , He, Xue , Luo, Guizhen , Liu, Jiayi , Wang, Zemiao , Zhu, Binling et al. A sensitive multicolor colorimetric aptasensor for on-site detection of total fentanyl and acetylfentanyl concentration based on horseradish peroxidase-regulated gold nanobipyramid growth . | MICROCHEMICAL JOURNAL , 2025 , 219 . |
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The development of gold nanobipyramid (AuNBP)-based multicolor colorimetric aptasensors offers significant advantages for enabling sensitive, accurate, and visual on-site detection. However, existing AuNBP growth-based strategies suffer from the inherent limitations of enzymes and the instability of AuNBPs that restrict their application in aptasensor construction. In this study, a multicolor colorimetric aptasensor based on a novel Gquadruplex (G4) DNAzyme-mediated AuNBP growth strategy was developed for the detection of quinclorac (QNC), a prevalent acid herbicide. First, complexes of magnetic bead (MB)-QNC aptamer (Qapt)-G4 DNA were formed through an MB-assisted competitive aptamer-binding system. In this system, the amount of MB-aptamer-G4 DNA complexes formed was directly proportional to the QNC concentration. These complexes subsequently facilitated the G4 DNAzyme-catalyzed oxidation of hydroquinone (HQ). The remaining HQ was then utilized to grow AuNBPs, leading to distinct multicolor variations. Under optimal conditions, the aptasensor exhibited nine discernible color changes corresponding to different QNC concentrations with a visual limit of detection (LOD) of 0.5 mu M and an instrumental LOD of 113 nM. Moreover, the aptasensor demonstrated excellent specificity and color stability. It was successfully used to determine QNC in river water, soil, and rice samples with recovery rates of 95.20 %-100.69 % and relative standard deviations (n = 5) < 3 %. These results indicate that it is a reliable and promising method for the rapid screening of QNC residues in environmental and food samples. Furthermore, this work provides a valuable reference strategy for transforming G4 DNAzyme-based colorimetric aptasensors into multicolor analytical platforms.
Keyword ：
Gold nanobipyramid growth Gold nanobipyramid growth G-quadruplex DNAzyme G-quadruplex DNAzyme Hydroquinone Hydroquinone Multicolor aptasensor Multicolor aptasensor Visual detection Visual detection
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| GB/T 7714 | Wang, Zemiao , Hu, Yang , Liu, Jiayi et al. A multicolor colorimetric aptasensor for visual detection of quinclorac based on G-quadruplex DNAzyme-mediated gold nanobipyramid growth [J]. | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 348 . |
| MLA | Wang, Zemiao et al. "A multicolor colorimetric aptasensor for visual detection of quinclorac based on G-quadruplex DNAzyme-mediated gold nanobipyramid growth" . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY 348 (2025) . |
| APA | Wang, Zemiao , Hu, Yang , Liu, Jiayi , Luo, Guizhen , He, Xue , Chen, Fengping et al. A multicolor colorimetric aptasensor for visual detection of quinclorac based on G-quadruplex DNAzyme-mediated gold nanobipyramid growth . | SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY , 2025 , 348 . |
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On-site quick detection of acetylcholinesterase (AChE) activity is of great significance for diagnosing nervous diseases, screening drugs, and monitoring poisoning. Herein, we developed a system for sensitively regulating gold nanobipyramid (AuNBP) morphology on a paper-based analytical device (PAD) via an Au+-disproportionation system, and further developed a high-resolution paper-based multicolor sensor (PMS) for both on-site visual detection and laboratory precision quantification of AChE activity using the system as a signal reporter and a dual-enzyme strategy as a receptor. The paper-based Au+ disproportionation-mediated AuNBP morphology system not only has excellent stability, reproducibility, and robust resistibility against reducing substances existing in biological samples but also can effectively suppress the color interference from reagents and greatly improve the sensitivity of regulating AuNBP morphology transformation, making it capable of generating long-term stable and more colorful/clearer color changes corresponding to Au+ concentrations. The developed PMS exhibited eight color changes corresponding to AChE activity within 0.0-1.0 U/L and the color changes could be stably maintained for 30 days, which makes both on-site visual detection and laboratory sensitive quantification possible. The PMS was successfully used to detect AChE activity in serum and erythrocyte samples with a visual detection limit (LOD) of 50 mU/L, a spectrometry LOD of 26 mU/L, and a recovery of 81-102%. The results obtained with the PMS were consistent with those obtained with Ellman's method, verifying its reliability. All of the above features make the PMS a promising assay for the rapid detection of AChE activity in clinical diagnosis. Importantly, the proposed paper-based Au+ disproportionation-mediated AuNBP morphology system can serve as a promising general signal reporter for developing various sensitive multicolor colorimetric sensors for target detection in various fields.
Keyword ：
colorimetricsensor colorimetricsensor enzyme enzyme gold nanocrystals gold nanocrystals nanobipyramids nanobipyramids paper-basedanalytical device paper-basedanalytical device
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| GB/T 7714 | Zhang, Feng , Wang, Zemiao , Fang, Ling et al. Regulating Gold Nanobipyramid Morphology via Au+-Disproportionation System on Paper Toward a Paper-Based Multicolor Sensor for Sensitive Visual Detection of Acetylcholinesterase Activity [J]. | ACS APPLIED NANO MATERIALS , 2024 , 7 (17) : 20933-20941 . |
| MLA | Zhang, Feng et al. "Regulating Gold Nanobipyramid Morphology via Au+-Disproportionation System on Paper Toward a Paper-Based Multicolor Sensor for Sensitive Visual Detection of Acetylcholinesterase Activity" . | ACS APPLIED NANO MATERIALS 7 . 17 (2024) : 20933-20941 . |
| APA | Zhang, Feng , Wang, Zemiao , Fang, Ling , Yang, Weijuan , Wang, Zongwen , Fu, FengFu . Regulating Gold Nanobipyramid Morphology via Au+-Disproportionation System on Paper Toward a Paper-Based Multicolor Sensor for Sensitive Visual Detection of Acetylcholinesterase Activity . | ACS APPLIED NANO MATERIALS , 2024 , 7 (17) , 20933-20941 . |
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A fast, simple, and sensitive method for the simultaneous determination of seven polyamines in Nephotettix cincticeps was developed based on ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-3Q-MS) together with liquid phase extraction. Polyamines in insect samples were extracted with HClO4 solution and then were separated and detected by UPLC-3Q-MS, which was equipped with a hydrophilic interaction liquid chromatography column, within 5 min without any derivatization procedure. The method has been successfully used to detect 7 polyamines in healthy and difluormethylornithine-treated adults of Nephotettix cincticeps with a method limit of detection and the method limit of quantitation of 24-139 pg/mg and 82-464 pg/mg, respectively, an intraday and interday relative standard deviation (RSD, n = 5) of 1.97-6.00% and 2.08-5.92% respectively, and a recovery of 86-115%. The success of this study provided a reliable method for the rapid and high-throughput detection of polyamines in the insect sample.
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| GB/T 7714 | Zhang, Mingwen , Zhao, Yunqiang , Wang, Zongwen et al. Rapid Analysis of Seven Polyamines in Nephotettix cincticeps by Using Ultra-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry [J]. | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY , 2024 , 2024 . |
| MLA | Zhang, Mingwen et al. "Rapid Analysis of Seven Polyamines in Nephotettix cincticeps by Using Ultra-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry" . | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY 2024 (2024) . |
| APA | Zhang, Mingwen , Zhao, Yunqiang , Wang, Zongwen , Cheng, Jintian . Rapid Analysis of Seven Polyamines in Nephotettix cincticeps by Using Ultra-Performance Liquid Chromatography-Triple Quadrupole Mass Spectrometry . | JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY , 2024 , 2024 . |
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Quinolones, a widely used class of antibiotics, present significant environmental and health concerns if they excessively remain in the environment and in food. Aptamers specific to quinolones can be applied as bioreceptors for the detection of quinolone residues in the environment and food. The quinolone family contains dozens of different individuals that share the same core structure coupled with various substituents at six different positions. The diversity and complexity of the substitution sites make it a challenge to choose a set of representative molecules that encompass all the desired sites and preserve the core molecular framework for the screening of quinolone-specific aptamers via systematic evolution of ligands by exponential enrichment (SELEX). To address this challenge, we introduce a novel parallel-series strategy guided by Liebig's law for isolating quinolone-specific cross-reactive aptamers by using the library-immobilized SELEX method. Through this approach, we successfully identified 5 aptamers (Apt.AQ01-Apt.AQ05) with high binding affinity and excellent specificity to 24 different quinolone individuals. Among them, Apt.AQ03 showcased optimal performance with affinities ranging from 0.14 to 1.07 mu M across the comprehensive set of 24 quinolones, exhibiting excellent specificity against nontarget interferents. The binding performance of Apt.AQ03 was further characterized with microscale thermophoresis, circular dichroism spectra, and an exonuclease digestion assay. By using Apt.AQ03 as a bioreceptor, a fluorescence resonance energy transfer (FRET) aptasensor was developed for the detection of 24 quinolones in milk, achieving a remarkable detection limit of 14.5-21.8 ng/mL. This work not only establishes a robust and effective strategy for selecting cross-reactive aptamers applicable to other small-molecule families but also provides high-quality aptamers for developing various high-throughput and reliable methods for the detection of multiple quinolone residues in food.
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| GB/T 7714 | Yang, Weijuan , Luo, Dongdong , Zheng, Siyu et al. Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy [J]. | ANALYTICAL CHEMISTRY , 2024 , 96 (21) : 8576-8585 . |
| MLA | Yang, Weijuan et al. "Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy" . | ANALYTICAL CHEMISTRY 96 . 21 (2024) : 8576-8585 . |
| APA | Yang, Weijuan , Luo, Dongdong , Zheng, Siyu , Zhang, Yiru , Wang, Zongwen , Fu, Fengfu . Screening of Cross-Reactive Aptamers for the Detection of 24 Quinolones by Using a Liebig's Law-Guided Parallel-Series Strategy . | ANALYTICAL CHEMISTRY , 2024 , 96 (21) , 8576-8585 . |
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On-site visual detection of organophosphorus pesticides (OPs) is crucial for safeguarding environmental and food safety. This study presents a robust, sensitive, and portable paper-based multicolor sensor (PMS) for the on-site detection of OPs. The PMS involves a novel Au+-induced gold nanobipyramids (AuNBPs) morphology transformation reaction within a paper-based analytical device (PAD) coupled with a bienzymatic hydrolysis system. The Au+-induced reaction sensitively regulates the aspect ratio of AuNBPs through Au+ disproportionation, which decreases the length of AuNBPs and simultaneously increases the width, accompanied by distinct multicolor changes. Upon performing in the PAD incorporating easily-prepared AuNBPs-deposited nylon membranes, a metal heater, and an oscillator, the Au+-induced reaction exhibits excellent reaction sensitivity, efficiency, reproducibility, AuNBPs stability, and color uniformity. In the bienzymatic hydrolysis system, acetylcholinesterase (AChE) and choline oxidase hydrolyze acetylcholine to product H2O2, which consumes tris(2-carboxyethyl)phosphine hydrochloride (TCEP), maintaining free Au+ for the disproportionation in the Au+-induced system, while the presence of OPs inhibits AChE activity, preserving TCEP to chelate Au+ and hindering the disproportionation. Under optimized conditions, the PMS exhibited eight dichlorvos concentration-corresponding color changes with a visual detection limit of 30 mu g/L and a spectrometry detection limit of 8.1 mu g/L. It achieved recoveries of 85.4-101.9 % in river water, drinking water, rice, and Chinese cabbage samples with relative standard deviations (n = 5) < 6 %. Thus, this study offers a portable, sensitive, reliable, and practical on-site detection method for OPs. Additionally, the proposed Au+-induced AuNBPs morphology transformation system within the PAD offers promising alternative strategies for developing other AuNBPs-based colorimetric sensors.
Keyword ：
Acetylcholinesterase Acetylcholinesterase Dichlorvos Dichlorvos Gold nanobipyramids Gold nanobipyramids Paper-based analytical device Paper-based analytical device Visual detection Visual detection
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| GB/T 7714 | Zhang, Feng , Ren, Enxi , Wang, Zemiao et al. Portable paper-based multicolor sensor for sensitive on-site detection of organophosphorus pesticides using a high-efficiency Au+-induced gold nanobipyramids aspect ratio regulation strategy [J]. | MICROCHEMICAL JOURNAL , 2024 , 207 . |
| MLA | Zhang, Feng et al. "Portable paper-based multicolor sensor for sensitive on-site detection of organophosphorus pesticides using a high-efficiency Au+-induced gold nanobipyramids aspect ratio regulation strategy" . | MICROCHEMICAL JOURNAL 207 (2024) . |
| APA | Zhang, Feng , Ren, Enxi , Wang, Zemiao , Fang, Ling , Yang, Weijuan , Zhang, Liaoyuan et al. Portable paper-based multicolor sensor for sensitive on-site detection of organophosphorus pesticides using a high-efficiency Au+-induced gold nanobipyramids aspect ratio regulation strategy . | MICROCHEMICAL JOURNAL , 2024 , 207 . |
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On-site quantitative detection of organophosphorus pesticides (OPs) is crucial for safeguarding food and public safety. This study presents a novel acetylcholinesterase (AChE)-mediated paper-based Au(3+)etching of gold nanobipyramids (AuNBPs) system. The system employs a long-term storable AuNBPs-deposited nylon membrane embedded within a portable and temperature-controlled paper-based analytical device. This system, coupled with a colorimeter-based quantitative method, enables the development of a practical paper-based multicolor sensor (PMS) for on-site quantitative detection of three common OPs (paraoxon, dichlorvos, and trichlorfon). In the absence of OPs, AChE hydrolyzes acetylthiocholine to thiocholine, which reduces Au(3+)to A(u+). The presence of OPs inhibits AChE activity, thereby preserving Au3+ to etch AuNBPs on nylon membranes, accompanied by multicolor changes. These color changes can be simply quantified by measuring the a* parameter of the CIELab color space using a portable colorimeter. Under optimal conditions, the PMS displayed eight OPs-corresponding color changes with a minimum detectable concentration of 1.0-10 mu g/L (visual observation) and limits of detection of 0.8-7.2 mu g/L (colorimeter) and 0.2-3.4 mu g/L (UV-vis spectrometry). The PMS successfully determined the OPs in vegetable and rice samples with recoveries of 89.0-109 % and RSDs (n = 5) of <6 %. These results were consistent with those obtained using the HPLC-MS method. The PMS demonstrates excellent portability, AuNBPs stability, detection sensitivity, and reproducibility, making it a promising tool for the on-site quantitative detection of OPs residues in food. Furthermore, the paper-based etching system coupled with the colorimeter-based quantitative method provides a valuable reference to develop practical PMSs for various targets in diverse fields.
Keyword ：
Colorimeter Colorimeter Colorimetric biosensor Colorimetric biosensor Gold nanobipyramids-deposited membrane Gold nanobipyramids-deposited membrane Paper-based analytical device Paper-based analytical device The CIELab color space The CIELab color space
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| GB/T 7714 | Zhang, Feng , Gao, Yu , Ren, Enxi et al. Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space [J]. | TALANTA , 2024 , 281 . |
| MLA | Zhang, Feng et al. "Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space" . | TALANTA 281 (2024) . |
| APA | Zhang, Feng , Gao, Yu , Ren, Enxi , Fang, Ling , Yang, Weijuan , Zhang, Liaoyuan et al. Paper-based multicolor sensor for on-site quantitative detection of organophosphate pesticides based on acetylcholinesterase-mediated paper-based Au3+-etching of gold nanobipyramids and CIELab color space . | TALANTA , 2024 , 281 . |
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