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学者姓名：汤宝珍
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Abstract ：
Serpins (serine protease inhibitors) constitute a superfamily of proteins with functional diversity and unusual conformational flexibility. In insects, serpins act as multiple inhibitors, by forming inactive acyl-enzyme complexes, in regulating Sp & auml;tzles activation, phenoloxidases (POs) activity, and other cytokines. In this study, we present the cloning and characterization of Octodonta nipae serpin2 (OnSPN2), a 415 residues protein homologous to Tenebrio molitor 42Dd-like. Notably, OnSPN2 features an arginine residue (R364) at the P1 position, and additional arginine residues (R362, R367) at the P3 and P3 ' positions, respectively which is crucial for protease inhibition. Immunohistochemistry (IHC) and Western blot analyses revealed that OnSPN2 is primarily synthesized in plasmatocytes and then released into the plasma to exert its function. RNA interference results indicated that OnSPN2 knockdown may depress serine protease in melanization and remarkably increase the transcript level of Attacin in hemolymph, but its messenger RNA levels were not changed upon immune induction. Reciprocal co-immunoprecipitation assay results confirmed that OnSPN2 binds to OnPPAF1 and OnSP8, indicating its role as a negative regulator in the PO and AMP pathway. Intriguingly, several cathepsin-L isoforms were identified in the OnSPN2 immunoprecipitated samples. The cathepsin-L inhibition assays and protein-protein docking results, identified cathepsin-L as a potential target of OnSPN2. These results indicate that OnSPN2 is produced as an intracellular resident and additionally is associated with the PO and AMP pathway. OnSPN2 represents a multiple defense tool that may provide multiple antiproteolytic functions.
Keyword ：
cathepsin-L cathepsin-L Octodonta nipae Octodonta nipae phenoloxidase cascade phenoloxidase cascade serine protease serine protease serpin serpin
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| GB/T 7714 | Zhang, Huajian , Xu, Jiawei , Chen, Mintao et al. The OnSPN2 from the nipa palm hispid beetle Octodonta nipae is a multipurpose defense tool against proteases from different peptidase families [J]. | INSECT SCIENCE , 2025 . |
| MLA | Zhang, Huajian et al. "The OnSPN2 from the nipa palm hispid beetle Octodonta nipae is a multipurpose defense tool against proteases from different peptidase families" . | INSECT SCIENCE (2025) . |
| APA | Zhang, Huajian , Xu, Jiawei , Chen, Mintao , Yin, Jiawei , Hou, Youming , Tang, Baozhen . The OnSPN2 from the nipa palm hispid beetle Octodonta nipae is a multipurpose defense tool against proteases from different peptidase families . | INSECT SCIENCE , 2025 . |
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BACKGROUNDParasitoid wasps rely on precise immune manipulation to establish infections in their hosts. Tetrastichus brontispae must overcome the robust melanization response of its palm pest host Octodonta nipae, yet the molecular mechanisms regulating to this process remain poorly characterized, particularly regarding microRNA (miRNA)-mediated control of the phenoloxidase (PO) cascade.RESULTSBioinformatic analysis of the O. nipae genome identified 11 melanization-associated miRNAs targeting key immune genes. Dual luciferase detection system assay and RNA binding protein immunoprecipitation results revealed that On-miR-182-5p and On-miR-281-3p regulate the melanization pathway through distinct mechanisms: On-miR-182-5p binds the 5 ' untranslated region (UTR) of OnSPN1, while On-miR-281-3p targets the 3 ' CDS of OnPPO1. Antagomir-mediated knockdown of miR-281-3p increased OnPPO1 messenger RNA (mRNA) levels by 52% and elevated PO activity by 68%, whereas miR-182-5p agomir treatment unexpectedly up-regulated OnSPN1 expression 2.4-fold. Disrupting this regulatory network significantly impaired T. brontispae fitness, reducing emergence rates by 27.3%, skewing offspring sex ratios to 3.8 females per male, and prolonging development time by 48 h.CONCLUSIONOur study reveals a sophisticated immune manipulation strategy where T. brontispae hijacks host miR-182-5p (activator) and miR-281-3p (suppressor) to bidirectionally regulate the PO cascade, simultaneously inhibiting melanization while maintaining microbial defense capacity. These findings provide novel targets for improving biological control strategies against invasive palm pests through miRNA-based interventions. (c) 2025 Society of Chemical Industry.
Keyword ：
melanization melanization microRNA microRNA Octodonta nipae Octodonta nipae phenoloxidase cascade phenoloxidase cascade Tetrastichus brontispae Tetrastichus brontispae
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| GB/T 7714 | Zhang, Huajian , Liao, Guiming , Yin, Jiawei et al. Tetrastichus brontispae exploits host-derived On-miR-281-3p to suppress the phenoloxidase pathway and facilitate its development [J]. | PEST MANAGEMENT SCIENCE , 2025 , 81 (12) : 8151-8162 . |
| MLA | Zhang, Huajian et al. "Tetrastichus brontispae exploits host-derived On-miR-281-3p to suppress the phenoloxidase pathway and facilitate its development" . | PEST MANAGEMENT SCIENCE 81 . 12 (2025) : 8151-8162 . |
| APA | Zhang, Huajian , Liao, Guiming , Yin, Jiawei , Xu, Jiawei , Hou, Youming , Tang, Baozhen . Tetrastichus brontispae exploits host-derived On-miR-281-3p to suppress the phenoloxidase pathway and facilitate its development . | PEST MANAGEMENT SCIENCE , 2025 , 81 (12) , 8151-8162 . |
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Abstract ：
本发明提供了一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法。采用界面聚合法制备茚虫威聚脲微胶囊。使用聚脲微胶囊茚虫威毒饵进行红火蚁防治，通过聚脲微胶囊茚虫威毒饵的使用，使茚虫威通过缓慢释放，有效提升毒饵在红火蚁巢穴内的传播范围和覆盖深度，同时延长毒饵的持效期，提高对红火蚁的持续防控效果。
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| GB/T 7714 | 汤宝珍 , 韩延伟 , 张大侠 et al. 一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法 : CN202510489519.9[P]. | 2025-04-18 . |
| MLA | 汤宝珍 et al. "一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法" : CN202510489519.9. | 2025-04-18 . |
| APA | 汤宝珍 , 韩延伟 , 张大侠 , 侯有明 , 廖贵明 , 张婧娴 et al. 一种聚脲微胶囊茚虫威红火蚁毒饵及其制备方法 : CN202510489519.9. | 2025-04-18 . |
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茶色疯蚁Nylanderia fulva Mayr是一种危险性入侵害虫，原产南美洲，已入侵美国的德克萨斯、密西西比、路易斯安那和佛罗里达等州，我国尚无分布记录。该虫食性复杂，适应性强，主要危害电器设备和电路，频繁的全球贸易及国际运输将加速该害虫的传播和扩散，存在较大的入侵风险。该文介绍了茶色疯蚁的分类地位、分布、形态特征、与近源种的形态区别和危害等，提出我国需要高度警惕，严防茶色疯蚁入侵。
Keyword ：
为害特点 为害特点 入侵 入侵 分布 分布 形态特征 形态特征 茶色疯蚁 茶色疯蚁
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| GB/T 7714 | 陈智明 , 张翔 , 夏雪 et al. 警惕危险性害虫茶色疯蚁Nylanderia fulva Mayr入侵 [J]. | 应用昆虫学报 , 2025 , 62 (02) : 522-527 . |
| MLA | 陈智明 et al. "警惕危险性害虫茶色疯蚁Nylanderia fulva Mayr入侵" . | 应用昆虫学报 62 . 02 (2025) : 522-527 . |
| APA | 陈智明 , 张翔 , 夏雪 , 汤宝珍 , 林静 , 范婷婷 et al. 警惕危险性害虫茶色疯蚁Nylanderia fulva Mayr入侵 . | 应用昆虫学报 , 2025 , 62 (02) , 522-527 . |
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本发明公开了一种负载茚虫威的pH‑酶双响应可控缓释材料，其是先采用“一锅法”将农药茚虫威封装在多空隙的骨架结构材料ZIF‑8中，然后通过静电吸附在其颗粒表面依次包覆羧化壳聚糖及壳聚糖而制得。所制得的IDC‑ZIF‑8@CCS/CS对茚虫威的包封率约为70%，将其与红火蚁饵剂混合，可制得用于红火蚁防治的毒饵。本发明所制备的纳米材料基于羧化壳聚糖与壳聚糖的双重包覆材料，具有更好的封装性，能满足农药控制释放用途；纳米材料在雨水中具有高稳定性，有助于减少残留农药对环境的污染；利用其制备的毒饵能够有效减缓红火蚁传毒蚁的死亡速率，有助于延迟因大量工蚁死亡引起的蚁群惊扰和迁移现象，提高传毒效率。
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| GB/T 7714 | 杨玮娟 , 张艺茹 , 汤宝珍 et al. 负载茚虫威的pH-酶双响应可控缓释材料及其制备与应用 : CN202410948419.3[P]. | 2024-07-16 . |
| MLA | 杨玮娟 et al. "负载茚虫威的pH-酶双响应可控缓释材料及其制备与应用" : CN202410948419.3. | 2024-07-16 . |
| APA | 杨玮娟 , 张艺茹 , 汤宝珍 , 侯有明 . 负载茚虫威的pH-酶双响应可控缓释材料及其制备与应用 : CN202410948419.3. | 2024-07-16 . |
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The invasive golden apple snail, Pomacea canaliculate, threatens agriculture and public health globally. This study systematically investigates the molluscicidal mechanism of theasaponin E1 (TSE1), a tea saponin monomer. TSE1 exhibited concentration- and time-dependent toxicity. Transcriptomics revealed dysregulation of cholesterol homeostasis, including marked upregulation of squalene monooxygenase, lanosterol synthase, and cholesterol catabolic enzymes (CYP3A24, CYP2U1). Concurrently, sphingolipid metabolism exhibited dual perturbation, with both sphingomyelin accumulation and ceramide elevation mediated by differential expression of DEGS2 and MOXD1. Ultrastructural analysis showed mitochondrial cristae dissolution and membrane rupture, with ferroptosis activation through voltage-dependent anion channel 2 upregulation and oxidative stress. Notably, granulocytes displayed distinct resistance to TSE1-induced cytotoxicity, suggesting cell-type specific protective mechanisms. These findings establish that TSE1 exerts its molluscicidal effect through coordinated disruption of lipid homeostasis, culminating in iron-dependent cell death. The conserved ferroptotic pathway and cell-selective vulnerabilities identified in this study provide critical insights for developing targeted, environmentally sustainable molluscicides.
Keyword ：
cholesterol metabolism cholesterol metabolism ferroptosis ferroptosis hemocyte hemocyte Pomacea canaliculata Pomacea canaliculata sphingolipid metabolism sphingolipid metabolism tea saponin tea saponin
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| GB/T 7714 | Ma, Cheng-Jun , Liao, Gui-Ming , Han, Yan-Wei et al. Theasaponin E1 Induce Ferroptotic Cell Death in Pomacea canaliculata through Coordinated Disruption of Cholesterol Homeostasis and Sphingolipid Metabolism [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (46) : 29581-29592 . |
| MLA | Ma, Cheng-Jun et al. "Theasaponin E1 Induce Ferroptotic Cell Death in Pomacea canaliculata through Coordinated Disruption of Cholesterol Homeostasis and Sphingolipid Metabolism" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 46 (2025) : 29581-29592 . |
| APA | Ma, Cheng-Jun , Liao, Gui-Ming , Han, Yan-Wei , Chen, Pei , Wang, Xin-Ao , Li, Jia-Wei et al. Theasaponin E1 Induce Ferroptotic Cell Death in Pomacea canaliculata through Coordinated Disruption of Cholesterol Homeostasis and Sphingolipid Metabolism . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (46) , 29581-29592 . |
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Exploring the function of the host immune system can help to understand the host-parasitoid interaction. Prophenoloxidase (PPO) is crucial in defensive melanization during the encapsulation of wasp eggs. However, the existence of multiple PPO sequences increases the difficulty of exploring the specific functions of individual PPOs. We previously identified three PPOs in the nipa palm hispid beetle, Octodonta nipae. Our current work showed that OnPPO1 and OnPPO2 possessed the typical characteristics of the type III copper family, but OnPPO3 lacked the conserved histidine residues, and its active sites were substituted with Gln. OnPPOs showed the highest expression in hemocytes, but OnPPO3 presented extremely low abundance compared with that of OnPPO1 and OnPPO2, and only OnPPO1 showed a quick response after wasp infection. OnPPO1 knockdown decreased the encapsulation index and inhibited melanization, whereas silencing of OnPPO3 appeared to have no adverse effect on encapsulation and melanization, and silencing of OnPPO2 presented low RNAi efficiency. Moreover, the cleavage of recombinant OnPPO1 produced a 62 kDa fragment with high PO activity. OnPPO1 could be produced by oenocytoids, granulocytes and plasmatocytes, and was distributed around wasp eggs during capsule formation. Overall, our results indicate that proteolytic cleavage of OnPPO1 plays key roles in the melanized encapsulation of wasp eggs. This finding illuminates the mechanism of PPO activation in this invasive beetle and provides guidance for its biological control.
Keyword ：
Encapsulation Encapsulation Melanization Melanization Octodonta nipae Octodonta nipae Parasitoid Parasitoid Prophenoloxidase Prophenoloxidase
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| GB/T 7714 | Zhang, Xia-Fang , Cui, Wen , Wang, Mou-Jun et al. Role of prophenoloxidase 1 from the beetle Octodonta nipae in melanized encapsulation of a wasp egg [J]. | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY , 2024 , 150 . |
| MLA | Zhang, Xia-Fang et al. "Role of prophenoloxidase 1 from the beetle Octodonta nipae in melanized encapsulation of a wasp egg" . | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 150 (2024) . |
| APA | Zhang, Xia-Fang , Cui, Wen , Wang, Mou-Jun , Zhou, Yan , Fu, Ting-Ting , Jiang, Kun et al. Role of prophenoloxidase 1 from the beetle Octodonta nipae in melanized encapsulation of a wasp egg . | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY , 2024 , 150 . |
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RNAi plays a crucial role in insect gene function research and pest control field. Nonetheless, the variable efficiency of RNAi across diverse insects and off-target effects also limited its further application. In this study, we cloned six essential housekeeping genes from Solenopsis invicta and conducted RNAi experiments by orally administering dsRNA. Then, we found that mixing with liposomes significantly enhanced the RNAi efficiency by targeting for SiV-ATPaseE. Additionally, we observed a certain lethal effect of this dsRNA on queens by our established RNAi system. Furthermore, no strict sequence-related off-target effects were detected. Finally, the RNAi effect of large-scale bacteria expressing dsRNA was successfully confirmed for controlling S. invicta. In summary, this study established an RNAi system for S. invicta and provided a research template for the future development of nucleic acid drugs based on RNAi.
Keyword ：
bacterial-expressed bacterial-expressed nanocarrier nanocarrier off-target effect off-target effect red imported fire ant red imported fire ant RNAi RNAi
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| GB/T 7714 | Wang, Jin-da , Chen, Yao-hui , Zhang, Ya-xin et al. Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (19) : 10936-10943 . |
| MLA | Wang, Jin-da et al. "Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 19 (2024) : 10936-10943 . |
| APA | Wang, Jin-da , Chen, Yao-hui , Zhang, Ya-xin , Lin, Jin-wen , Gao, San-ji , Tang, Bao-zhen et al. Establishment of RNAi-Mediated Pest Control Method for Red Imported Fire Ant, Solenopsis invicta . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (19) , 10936-10943 . |
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【目的】探究不同品系椰扁甲啮小蜂（水椰八角铁甲繁育的Tb-On品系，椰心叶甲繁育的Tb-Bl品系）寄生对水椰八角铁甲和椰心叶甲蛹脂滴形态的影响，为解析该蜂调控靶标寄主脂肪体代谢过程提供科学依据。【方法】利用油红O染色观察椰扁甲啮小蜂幼体内脂滴以及被寄生的水椰八角铁甲和椰心叶甲蛹脂肪体组织结构的动态变化。【结果】在相同发育时段（在寄主体内分别发育至48、72和96 h）内，Tb-On品系与Tb-Bl品系幼体脂滴的形态及分布无明显区别，在发育早期仅头部及表皮可见少量脂滴，随着发育时间的延长，脂滴逐渐充满整个幼体体腔。不同品系椰扁甲啮小蜂分别寄生同一种类寄主时，不同处理寄主间的脂肪体组织结构变化无明显差异；但同一品系椰扁甲啮小蜂寄生不同寄主时，不同处理寄主间的脂肪体组织结构变化存在明显差异，水椰八角铁甲蛹被寄生后24 h脂滴逐步裂解，脂滴的染色面积上升而光密度值下降，而椰心叶甲蛹在被寄生后48 h其体内的脂滴才逐步裂解。随着寄生蜂幼体的不断发育，2种寄主蛹内脂滴染色面积和光密度值均呈持续下降趋势，脂滴着色逐渐变浅甚至消失。【结论】不同品系椰扁甲啮小蜂幼体脂滴的形态及分布在相同发育时段内无明显区别；被同一品系椰扁甲啮小蜂寄生后，水椰八角铁甲蛹脂肪体组织的裂解早于椰心叶甲，裂解出的脂滴可为椰扁甲啮小蜂幼体的生长发育提供脂类营养物质来源。
Keyword ：
寄生 寄生 幼蜂 幼蜂 椰心叶甲 椰心叶甲 椰扁甲啮小蜂 椰扁甲啮小蜂 水椰八角铁甲 水椰八角铁甲 脂滴 脂滴
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| GB/T 7714 | 付浪 , 孟娥 , 付婷婷 et al. 不同品系椰扁甲啮小蜂寄生对2种寄主脂肪体组织的影响 [J]. | 生物安全学报(中英文) , 2024 , 33 (02) : 123-131 . |
| MLA | 付浪 et al. "不同品系椰扁甲啮小蜂寄生对2种寄主脂肪体组织的影响" . | 生物安全学报(中英文) 33 . 02 (2024) : 123-131 . |
| APA | 付浪 , 孟娥 , 付婷婷 , 乔婷 , 吴江林 , 林亚平 et al. 不同品系椰扁甲啮小蜂寄生对2种寄主脂肪体组织的影响 . | 生物安全学报(中英文) , 2024 , 33 (02) , 123-131 . |
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Simple Summary Endoparasitic wasps are nature's pest controllers, targeting various harmful insects. They lay their eggs inside these pests and release special substances (like viruses or venoms) that change the way the pest's immune system works. However, we are still uncovering exactly how these changes happen. Our research looks into how certain wasps, which do not use these viruses, can still affect their host's immune system. We studied this using a specific wasp-beetle pair as an example. We found that these wasps can control a crucial part of the beetle's immune response through a process that modifies the beetle's genetic material, specifically by adjusting certain markers on the genes that are important for immunity. This discovery sheds light on the sophisticated ways wasps can suppress the immune systems of their hosts, making them more effective at controlling pest populations.Abstract Endoparasitoids are insects that develop within other insects, employing unique strategies to enhance their offspring's survival. They inject polydnavirus and/or venom into their hosts along with eggs, effectively suppressing the host's immune system. Polydnavirus from Braconidae and Ichneumonidae wasps can integrate into the host's genome to express viral genes using the host's transcription systems. However, the ability of parasitoids without polydnavirus to manipulate host gene expression remains unclear. Lysine acetylation (LysAc), a post-translational modification critical for gene regulation, is hypothesized to be used by endoparasitoids lacking polydnavirus. We utilized the Chalcidoidea wasp Tetrastichus brontispae, which lacks polydnavirus, as an idiobiont endoparasitoid model to test this hypothesis, with pupae of the nipa palm hispid beetle Octodonta nipae as the host. Parasitism by T. brontispae resulted in the reduced expression of histone deacetylase Rpd3 and elevated levels of LysAc modification at histones H3.3K9 and H3.3K14 through proteomics and LysAc modification omics. The knockdown of Rpd3 increased the expression level of OnPPAF1 and OnPPO involved in the phenoloxidase cascade, leading to melanization in the host body whereby it resembled a mummified parasitized pupa and ultimately causing pupa death. This study enhances our understanding of how endoparasitoids employ histone acetylation to regulate immunity-related genes, offering valuable insights into their survival strategies.
Keyword ：
epigenetics epigenetics histone acetylation histone acetylation melanization melanization Octodonta nipae Octodonta nipae parasitism parasitism Tetrastichus brontispae Tetrastichus brontispae
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| GB/T 7714 | Jiang, Kun , Zhou, Yan , Cui, Wen et al. Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp [J]. | INSECTS , 2024 , 15 (3) . |
| MLA | Jiang, Kun et al. "Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp" . | INSECTS 15 . 3 (2024) . |
| APA | Jiang, Kun , Zhou, Yan , Cui, Wen , Han, Yan-Wei , Chen, Pei , Liao, Gui-Ming et al. Histone Acetylation Enhancing Host Melanization in Response to Parasitism by an Endoparasitoid Wasp . | INSECTS , 2024 , 15 (3) . |
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