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学者姓名：张洁
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Abstract ：
Rice viral diseases pose severe threats to global food security, with over 20 viruses identified in China alone. The advent of high-throughput sequencing has accelerated the discovery of novel viruses in cultivated and wild rice, unveiling previously undetected threats. This review systematically summarises newly discovered rice viruses over the past decade, analyzing their genomic characteristics, transmission modes, and pathogenic mechanisms. Key findings include the identification of rice stripe mosaic virus, rice tiller inhibition virus (RTIV), RTIV2 and rice curl dwarf-associated virus, among others, highlighting their interactions with host immunity and insect vectors. Notably, emerging viruses exhibit novel infection strategies, including interference with RNA silencing, hormone signalling, and autophagy pathways. Moreover, mixed infections and cross-species transmission raise concerns about evolving disease dynamics. Understanding these viral threats is crucial for developing integrated disease management strategies, including resistant cultivars and vector control measures. This review provides a comprehensive resource for advancing rice virology research and guiding future efforts in monitoring and mitigating viral diseases affecting global rice production.
Keyword ：
cultivated rice cultivated rice high-throughput sequencing high-throughput sequencing new viruses new viruses wild rice wild rice
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| GB/T 7714 | Ding, Xinlun , Wang, Feng , Liu, Pingping et al. Emerging Viral Threats in Rice: A Decade of Discovery and Implications for Crop Protection [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) : 8702-8711 . |
| MLA | Ding, Xinlun et al. "Emerging Viral Threats in Rice: A Decade of Discovery and Implications for Crop Protection" . | PLANT CELL AND ENVIRONMENT 48 . 12 (2025) : 8702-8711 . |
| APA | Ding, Xinlun , Wang, Feng , Liu, Pingping , Zhang, Jie , Wu, Zujian , Han, Yan-Hong et al. Emerging Viral Threats in Rice: A Decade of Discovery and Implications for Crop Protection . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (12) , 8702-8711 . |
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The begomoviral V2 protein is known to be multifunctional, including its interaction with and inhibition of CYP1, a papain-like cysteine protease (PLCP). However, the effect of this interaction on viral pathogenicity remains unclear. Cotton leaf curl Multan virus (CLCuMuV), a typical monopartite begomovirus associated with a betasatellite, is one of the main pathogens responsible for cotton leaf curl disease. This study verifies the interaction between CLCuMuV V2 and NbCP15, a PLCP homologue in Nicotiana benthamiana. The results show that V2 can be cleaved by NbCP15 in vitro, with the N-terminal cleavage site located between the second and third amino acids. Using an Agrobacterium-mediated inoculation method, we investigated the influence of cleavage sites on viral pathogenicity. The findings indicate that mutation of the third amino acid in V2 (V2D3A) reduced the pathogenicity of both heterologous PVX and CLCuMuV. Additionally, the NbCP15 gene mutation in N. benthamiana (nbcp15) also resulted in reduced CLCuMuV pathogenicity. These results suggest that CLCuMuV V2 may promote viral infection through its interaction with plant PLCPs.
Keyword ：
begomovirus begomovirus cotton leaf curl Multan virus cotton leaf curl Multan virus papain-like cysteine protease papain-like cysteine protease V2 protein V2 protein
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| GB/T 7714 | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences [J]. | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
| MLA | Zhang, Jie et al. "Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences" . | MOLECULAR PLANT PATHOLOGY 26 . 1 (2025) . |
| APA | Zhang, Jie , Shang, Pengxiang , Yuan, Linkai , Li, Dingshan , Liu, Shunmin , Du, Zhenguo et al. Papain-Like Cysteine Proteases Contribute to Functional Cleavage of Begomoviral V2 Effector Required for Relevant Virulences . | MOLECULAR PLANT PATHOLOGY , 2025 , 26 (1) . |
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本发明公开了一种基于电磁感应促进微生物代谢的方法及应用，其中一种基于电磁感应促进微生物代谢的方法，包括以下步骤：S1.得到电活性微生物的菌液；S2.将菌液加入去除电子供体培养基中，再加入导体材料、电子受体，得到培养体系；S3.在变化的磁场作用下，培养体系内形成电活性微生物‑导体杂化体系，驱动电活性微生物生长代谢及对电子受体进行转化。本发明克服了光驱动微生物‑光敏材料杂化体系方法中存在的需要额外添加牺牲试剂、光照产生的活性氧损伤微生物等缺点，本发明的方法相对简单、可操作性强、适用性强，在变化的磁场条件下，可选择地与不同微生物‑导体杂化体系组合使用，便于针对性地促进物质转化及污染物降解，具有较好的应用前景。
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| GB/T 7714 | 叶捷 , 胡静 , 张洁 et al. 一种基于电磁感应促进微生物代谢的方法及应用 : CN202411074917.6[P]. | 2024-08-07 . |
| MLA | 叶捷 et al. "一种基于电磁感应促进微生物代谢的方法及应用" : CN202411074917.6. | 2024-08-07 . |
| APA | 叶捷 , 胡静 , 张洁 , 周顺桂 . 一种基于电磁感应促进微生物代谢的方法及应用 : CN202411074917.6. | 2024-08-07 . |
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【目的】建立柑橘黄化脉明病毒（citrus yellow vein clearing virus, CYVCV）、柑橘衰退病毒（citrus tristeza virus, CTV）和啤酒花矮化类病毒（hop stunt viroid, HSVd）的多重RT-PCR检测体系。【方法】设计多重RT-PCR引物，分析其特异性，确定其最佳浓度比、最适退火温度及灵敏度，在此基础上对福建地区的柑橘样品进行检测。【结果】确定了CYVCV-F/R、CTV-F/R和HSVd-F/R等3对引物的最佳浓度比例为1∶1∶2，最适退火温度为52.9℃，灵敏度结果显示该体系可检测模板稀释到10-2的阳性样品。应用该体系对采自福建部分地区的157份柑橘样品进行检测，结果发现，CYVCV、CTV和HSVd的检出率分别为47.1%、56.7%和22.9%。【结论】成功建立了柑橘CYVCV、CTV和HSVd病原的多重RT-PCR检测方法，为该类病害的检测提供准确、快速的检测方法。
Keyword ：
啤酒花矮化类病毒 啤酒花矮化类病毒 多重RT-PCR 多重RT-PCR 柑橘衰退病毒 柑橘衰退病毒 柑橘黄化脉明病毒 柑橘黄化脉明病毒
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| GB/T 7714 | 袁琳凯 , 马崇欢 , 李丁山 et al. 柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用 [J]. | 福建农业学报 , 2024 , 39 (03) : 339-344 . |
| MLA | 袁琳凯 et al. "柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用" . | 福建农业学报 39 . 03 (2024) : 339-344 . |
| APA | 袁琳凯 , 马崇欢 , 李丁山 , 陈志炜 , 江宵烽 , 丁新伦 et al. 柑橘3种病毒类病原多重RT-PCR检测技术的建立及应用 . | 福建农业学报 , 2024 , 39 (03) , 339-344 . |
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Abstract ：
草莓病毒病是制约草莓产量和品质的重要因素之一。随着高通量测序技术在植物病毒检测和鉴定上的应用，一些新病毒相继在草莓上被发现。本文中综述了近十年来草莓上新发现的11种病毒，包括5种（黄瓜花叶病毒、菠菜潜隐病毒、番茄线虫传多面体病毒、橄榄潜伏病毒1和芸薹黄化病毒）以草莓为其新寄主和6种（草莓马铃薯叶卷病毒1、草莓病毒1、草莓病毒2、草莓病毒3、森林草莓相关病毒1和草莓病毒A）首次报道的新病毒，对这些新发现病毒的研究现状进行了阐述和总结，并对研究中存在的主要问题和今后的研究方向进行了讨论和展望，以期为生产上草莓病毒病诊断、检测、病害流行预测、预防和控制提供参考。
Keyword ：
病毒 病毒 草莓 草莓
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| GB/T 7714 | 丁新伦 , 张洁 , 吴祖建 . 草莓上新发现的病毒研究现状与展望 [J]. | 园艺学报 , 2024 , 51 (07) : 1639-1648 . |
| MLA | 丁新伦 et al. "草莓上新发现的病毒研究现状与展望" . | 园艺学报 51 . 07 (2024) : 1639-1648 . |
| APA | 丁新伦 , 张洁 , 吴祖建 . 草莓上新发现的病毒研究现状与展望 . | 园艺学报 , 2024 , 51 (07) , 1639-1648 . |
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The genome of a monopartite begomovirus, or the DNA-A component of a bipartite begomovirus, typically encodes six proteins: two on the viral strand (AV1/V1 and AV2/V2) and four on the complementary strand (AC1/ C1, AC2/C2, AC3/C3, AC4/C4). Recent studies, however, have identified additional begomoviral proteins with various functions. This paper reports that euphorbia leaf curl virus (EuLCV), a monopartite begomovirus, encodes a seventh protein, C5. Promoter activity of the upstream fragment of the EuLCV C5 gene was shown using a GUS expression vector. EuLCV C5 also enhanced the pathogenicity and accumulation of potato virus X (PVX) in Nicotiana benthamiana. Localization studies revealed that EuLCV C5 localizes to the cytoplasm and nucleus, forming granular structures on the cell membrane. Additionally, C5 acts as a post-transcriptional gene silencing (PTGS) suppressor. A C5 deletion mutant of EuLCV (EuLCV-Delta C5) exhibited reduced pathogenicity and viral accumulation compared to wild-type EuLCV in N. benthamiana.
Keyword ：
C5 protein C5 protein Euphorbia leaf curl virus Euphorbia leaf curl virus Pathogenicity determinant Pathogenicity determinant Post-transcriptional gene silencing Post-transcriptional gene silencing
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| GB/T 7714 | Zhang, Jie , Yuan, Linkai , Li, Dingshan et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor [J]. | VIROLOGY , 2024 , 600 . |
| MLA | Zhang, Jie et al. "The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor" . | VIROLOGY 600 (2024) . |
| APA | Zhang, Jie , Yuan, Linkai , Li, Dingshan , Yang, Xueying , Li, Jingyuan , Wu, Zujian et al. The C5 protein of euphorbia leaf curl virus is a virulence factor and gene silencing suppressor . | VIROLOGY , 2024 , 600 . |
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Geminiviruses are a family of single-stranded DNA viruses that cause significant yield losses in crop production worldwide. Transcription start site (TSS) mapping is crucial in understanding the gene expression mechanisms of geminiviruses. However, this often requires costly and laborious experiments. Rice stripe virus (RSV) has a mechanism called cap-snatching, whereby it cleaves cellular mRNAs and uses the 5 & PRIME; cleavage product, a cappedRNA leader (CRL), as primers for transcription. Our previous work demonstrated that RSV snatches CRLs from geminiviral mRNAs in co-infected plants, providing a convenient and powerful approach to map the TSSs of geminiviruses. However, co-infections are not always feasible for all geminiviruses. In this study, we evaluated the use of in vitro cap-snatching of RSV for the same purpose, using tomato yellow leaf curl virus (TYLCV) as an example. We incubated RNA extracted from TYLCV-infected plants with purified RSV ribonucleoproteins in a reaction mixture that supports in vitro cap-snatching of RSV. The RSV mRNAs produced in the reaction were deep sequenced. The CRLs snatched by RSV allowed us to locate 28 TSSs in TYLCV. These results provide support for using RSV's in vitro cap-snatching to map geminiviral TSSs.
Keyword ：
Cap -snatching Cap -snatching Geminivirus Geminivirus in vitro in vitro Rice stripe virus Rice stripe virus Transcription start sites Transcription start sites
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| GB/T 7714 | Lin, Wenzhong , Qiu, Ping , Xu, Yixing et al. Transcription start site mapping of geminiviruses using the in vitro cap-snatching of a tenuivirus [J]. | JOURNAL OF VIROLOGICAL METHODS , 2023 , 319 . |
| MLA | Lin, Wenzhong et al. "Transcription start site mapping of geminiviruses using the in vitro cap-snatching of a tenuivirus" . | JOURNAL OF VIROLOGICAL METHODS 319 (2023) . |
| APA | Lin, Wenzhong , Qiu, Ping , Xu, Yixing , Chen, Lihong , Wu, Zujian , Zhang, Jie et al. Transcription start site mapping of geminiviruses using the in vitro cap-snatching of a tenuivirus . | JOURNAL OF VIROLOGICAL METHODS , 2023 , 319 . |
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为了明确福建省三明地区柑橘病毒类病原(病毒和类病毒)种类，利用RT-PCR技术对其进行了鉴定和检测，并对其检出率进行了分析。结果表明，从207份柑橘叶片样品中检出柑橘衰退病毒(citrus tristeza virus, CTV)、柑橘黄化脉明病毒(citrus yellow vein clearing virus, CYVCV)、柑橘叶斑驳病毒(citrus leaf blotch virus, CLBV)和蚜虫致死麻痹病毒(aphid lethal paralysis virus, ALPV)等4种病毒以及柑橘曲叶类病毒(citrus bent leaf viroid, CBLVd)、啤酒花矮化类病毒(hop stunt viroid, HSVd)、柑橘矮化类病毒(citrus dwarfing viroid, CDVd)、柑橘类病毒Ⅴ(citrus viroidⅤ, CVdⅤ)和柑橘类病毒Ⅵ(citrus viroidⅥ, CVdⅥ)等5种类病毒。其中，CTV、CYVCV、CLBV和ALPV的检出率分别是71.01%、66.67%、0.97%和6.28%,CBLVd、HSVd、CDVd、CVdⅤ和CVdⅥ的检出率分别是7.25%、28.50%、13.04%、9.18%和6.76%。同时发现，CTV和CYVCV复合侵染的检出率为54.59%,而品种‘大分1号’的病毒检出率相对较低。本研究首次在柑橘叶片中检测到ALPV。
Keyword ：
柑橘 柑橘 检出率 检出率 病毒 病毒 类病毒 类病毒
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| GB/T 7714 | 张洁 , 王新 , 马崇欢 et al. 福建三明地区柑橘病毒类病原种类的鉴定及检测 [J]. | 植物保护 , 2023 , 49 (03) : 266-271 . |
| MLA | 张洁 et al. "福建三明地区柑橘病毒类病原种类的鉴定及检测" . | 植物保护 49 . 03 (2023) : 266-271 . |
| APA | 张洁 , 王新 , 马崇欢 , 李丁山 , 刘国坤 , 丁新伦 et al. 福建三明地区柑橘病毒类病原种类的鉴定及检测 . | 植物保护 , 2023 , 49 (03) , 266-271 . |
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木尔坦棉花曲叶病毒(CLCuMuV)是棉花曲叶病的主要病原之一，其V2蛋白在病毒致病过程中起重要作用。本研究对V2蛋白N端第4位脯氨酸在病毒致病过程中的作用进行了分析。通过构建马铃薯X病毒(PVX)异源表达载体(PVX-V2、PVX-V2~(P4A)),利用农杆菌介导的方法接种于本氏烟发现，PVX-V2能够增强PVX异源病毒的致病性，致使PVX在植物体内复制量增加，而PVX-V2~(P4A)对PVX异源病毒致病性的影响相对较小。通过构建V2基因突变体(ΔV2、V2~(P4A))侵染性克隆，利用农杆菌介导的方法接种于本氏烟发现，ΔV2、V2~(P4A)侵染性克隆引起的症状较野生型更弱。结果表明，CLCuMuV V2蛋白N端第4位脯氨酸在病毒侵染过程中起重要作用。
Keyword ：
PVX载体 PVX载体 V2蛋白 V2蛋白 侵染性克隆 侵染性克隆 木尔坦棉花曲叶病毒 木尔坦棉花曲叶病毒 致病性 致病性
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| GB/T 7714 | 尚鹏祥 , 张洁 , 张琦 et al. 木尔坦棉花曲叶病毒V2蛋白N端保守脯氨酸对病毒致病性的影响 [J]. | 福建农林大学学报(自然科学版) , 2023 , 52 (04) : 450-456 . |
| MLA | 尚鹏祥 et al. "木尔坦棉花曲叶病毒V2蛋白N端保守脯氨酸对病毒致病性的影响" . | 福建农林大学学报(自然科学版) 52 . 04 (2023) : 450-456 . |
| APA | 尚鹏祥 , 张洁 , 张琦 , 郑信诗 , 李景远 , 庄军 et al. 木尔坦棉花曲叶病毒V2蛋白N端保守脯氨酸对病毒致病性的影响 . | 福建农林大学学报(自然科学版) , 2023 , 52 (04) , 450-456 . |
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近年来，广东番木瓜曲叶病毒(Papaya leaf curl Guangdong virus, PaLCuGDV)和一品红曲叶病毒(Euphorbia leaf curl virus, EuLCV)经常在百香果(Passiflora edulis)上被鉴定。本研究从福建地区表现典型双生病毒危害症状的百香果上扩增得到该2种病毒的全长序列，分别为2 732和2 745 bp；为明确PaLCuGDV和EuLCV的致病性，利用同源重组技术构建了2种病毒的侵染性克隆，通过农杆菌(Agrobacterium tumefaciens)介导法接种本氏烟(Nicotiana benthamiana)，结果发现，接种EuLCV的本氏烟表现出明显矮化且顶端叶片畸形卷曲症状，受病害危害明显重于接种PaLCuGDV，后者仅表现新叶稍微卷曲和叶片黄化；2种病毒混合侵染，受病害危害重于单独接种EuLCV或PaLCuGDV。Southern blot结果显示，PaLCuGDV和EuLCV单独及混合接种的烟草均有较高的病毒积累量。本研究首次构建了PaLCuGDV的侵染性克隆，并发现EuLCV和PaLCuGDV复合侵染会加重病害症状。本研究为解析PaLCuGDV和EuLCV的致病机制提供参考。
Keyword ：
一品红曲叶病毒(EuLCV) 一品红曲叶病毒(EuLCV) 侵染性克隆 侵染性克隆 广东番木瓜曲叶病毒(PaLCuGDV) 广东番木瓜曲叶病毒(PaLCuGDV) 百香果 百香果
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| GB/T 7714 | 李景远 , 林文忠 , 李丁山 et al. 广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析 [J]. | 农业生物技术学报 , 2023 , 31 (09) : 1935-1943 . |
| MLA | 李景远 et al. "广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析" . | 农业生物技术学报 31 . 09 (2023) : 1935-1943 . |
| APA | 李景远 , 林文忠 , 李丁山 , 马崇欢 , 吴祖建 , 杜振国 et al. 广东番木瓜曲叶病毒和一品红曲叶病毒致病性分析 . | 农业生物技术学报 , 2023 , 31 (09) , 1935-1943 . |
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