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学者姓名：尤士骏
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Abstract ：
Insects depend on sophisticated olfaction for survival. The diamondback moth (Plutella xylostella), a devastating pest with rapid reproduction and insecticide resistance, requires novel control strategies. However, its odor recognition mechanisms, particularly for host-emitted alpha-Humulene, remain unclear. We investigated two odorant receptor genes, PxOR28 and PxOR31, which are highly expressed in female antennae. Cloning, qPCR, and CRISPR/Cas9 knockout demonstrated their essential role in mediating behavioral attraction to alpha-Humulene for oviposition. While alpha-Humulene activated wild-type antennae and attracted moths, mutants lacking both receptors exhibited no response, indicating functional redundancy. These findings elucidate P. xylostella's perception of alpha-Humulene and highlight chemosensory receptors or key odorants as targets for sustainable pest control.
Keyword ：
CRISPR/Cas9 CRISPR/Cas9 molecular docking molecular docking odorant receptors odorant receptors plutella xylostella plutella xylostella spawning preference spawning preference
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| GB/T 7714 | Yao, Shuyuan , Chen, Xuanhao , Chen, Yi et al. Functional Redundancy of Odorant Receptors in α-Humulene Mediates Ovipositional Behavior of a Cosmopolitan Insect Pest [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (46) : 29380-29391 . |
| MLA | Yao, Shuyuan et al. "Functional Redundancy of Odorant Receptors in α-Humulene Mediates Ovipositional Behavior of a Cosmopolitan Insect Pest" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 46 (2025) : 29380-29391 . |
| APA | Yao, Shuyuan , Chen, Xuanhao , Chen, Yi , Xie, Liangqian , Chen, Lina , Yue, Xiao et al. Functional Redundancy of Odorant Receptors in α-Humulene Mediates Ovipositional Behavior of a Cosmopolitan Insect Pest . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (46) , 29380-29391 . |
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Understanding the molecular mechanisms underlying temperature adaptation in agricultural pests is crucial for predicting their evolutionary responses to climate change. Here, we investigate the role of juvenile hormone esterase gene PxJHE in temperature adaptation of Plutella xylostella, a globally distributed pest. Spatial-temporal expression patterns demonstrates significantly reduced PxJHE transcript levels in hot-evolved (HS), and coldevolved (CS) strains across all tested temperatures compared to ancestral strain (AS). CRISPR/Cas9-mediated knockout strains (JHE-MU) exhibit substantially elevated juvenile hormone (JH) titers during development, accompanied by impaired extreme temperature tolerance and altered life history parameters. Biochemical analyses reveal that PxJHE deficiency leads to significant accumulation of lipids and total sugars, while simultaneously reducing antioxidant enzyme activities (SOD and CAT). Metabolomic profiling reveal that PxJHE deficiency causes extensive metabolic rewiring, particularly in lipid, carbohydrate and amino acid pathways. Gene expression analysis demonstrates that PxJHE knockout downregulates key metabolic enzymes including 6phosphofructokinase (PxPFK) and hormone-sensitive lipase (PxHSL), indicating impaired energy mobilization despite enhanced substrate storage. Our findings demonstrate that PxJHE modulates temperature adaptation through a multi-level regulatory mechanism involving JH signaling, metabolic network coordination, and antioxidant defense systems. This study provides novel insights into the genetic architecture of climate adaptation in agricultural pests, revealing the crucial interface between hormonal regulation, metabolic plasticity, and oxidative stress management in environmental adaptation.
Keyword ：
Diamondback moth Diamondback moth Environmental adaptation Environmental adaptation Global climate change Global climate change Juvenile hormone esterase Juvenile hormone esterase Metabolic plasticity Metabolic plasticity
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| GB/T 7714 | Lei, Gaoke , Zhou, Huiling , Yu, Guangyu et al. Metabolic network remodeling through PxJHE modulates temperature adaptation in a cosmopolitan insect [J]. | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 182 . |
| MLA | Lei, Gaoke et al. "Metabolic network remodeling through PxJHE modulates temperature adaptation in a cosmopolitan insect" . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 182 (2025) . |
| APA | Lei, Gaoke , Zhou, Huiling , Yu, Guangyu , Miao, Xin , Zhang, Yu , Ma, Zongyao et al. Metabolic network remodeling through PxJHE modulates temperature adaptation in a cosmopolitan insect . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 182 . |
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The olfactory system of insects plays a pivotal role in multiple, essential activities including feeding, mating, egg laying, and host localization. The capacity of odorant receptors to recognize odor molecules relies on odorant receptor co-receptors forming heterodimers. Here we report the successful engineering a homozygous mutant strain of diamondback moth ( Plutella xylostella) in which the odorant receptor co-receptor PxOrco was silenced using CRISPR/Cas9. This insect is a globally important crop pest for which novel control methods are urgently required. Behavioral assays demonstrated that PxOrco knockout males exhibited abolished courtship behaviors, inability to mate, and loss of selective preference for P. xylostella's key sex pheromone components. Whilst female mating behavior and fecundity remained unaffected by PxOrco knockout, oviposition response to leaf alcohol, a key cue for normal oviposition behavior, was lost. Electroantennography revealed drastically reduced responses to sex pheromones and plant volatiles in PxOrco-deficient adults but food location by larvae was unaffected. Moreover, expression analysis of PxOrco- deficient pheromone receptors (PRs) indicated varied regulation patterns, with down-regulation observed in several PRs in both sexes. These findings underscore the critical role of PxOrco in regulating multiple olfactory aspects in P. xylostella, including feeding, mating, and host location. Our study identifies the potential of disrupting the Orco gene in this and other pest species to provide novel avenues for future pest control.
Keyword ：
Electroantennography Electroantennography Mating disruption Mating disruption Odorant receptor co-receptors (orco) Odorant receptor co-receptors (orco) Oviposition behavior Oviposition behavior Pheromone Pheromone
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| GB/T 7714 | Chen, Xuanhao , Yao, Shuyuan , Xie, Liangqian et al. Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest [J]. | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 177 . |
| MLA | Chen, Xuanhao et al. "Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest" . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY 177 (2025) . |
| APA | Chen, Xuanhao , Yao, Shuyuan , Xie, Liangqian , Li, Jinyang , Xiong, Lei , Yang, Xiaozhen et al. Disruption of the odorant receptor co-receptor (Orco) reveals its critical role in multiple olfactory behaviors of a cosmopolitan pest . | INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY , 2025 , 177 . |
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Global climate change is characterized by increased extreme temperatures affecting insects at all trophic levels. Zinc finger proteins (ZFPs) are key regulators of gene expression and cell differentiation in eukaryotes, essential for stress resistance in both animals and plants. Using CRISPR/Cas9 for gene deletion, this study predicted and examined the structure of ZFP320 in the diamondback moth (Plutella xylostella) and investigated its function in temperature stress response through a comprehensive age-stage, two-sex life table analysis. We found ZFP320 encodes a 387 amino acid protein (43 kDa) with no transmembrane domains, featuring a ZnF-C2H2 domain. Quantitative fluorescence analysis showed that ZFP320 expression increased under high temperatures. ZFP320 knockout altered antioxidant gene expression, resulting in higher levels of superoxide dismutase and catalase in mutant strains compared with wild-type strain. Life table analysis revealed that the mutant strains had shorter fecundity and oviposition periods under both normal and high temperatures. Additionally, mutant strains exhibited lower parameters (r, lambda, R0), as well as reduced survival rates and critical thermal maxima. Notably, PxZFP320 plays a crucial role in temperature adaptation, paving the way for future investigations on the significance of ZFPs in P. xylostella's temperature tolerance.
Keyword ：
age-stage two-sex life table age-stage two-sex life table CRISPR/Cas9 CRISPR/Cas9 Plutella xylostella Plutella xylostella temperature adaptability temperature adaptability
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| GB/T 7714 | Miao, Xin , Cao, Fang , Yu, Xiao-Fei et al. A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest [J]. | OPEN BIOLOGY , 2025 , 15 (4) . |
| MLA | Miao, Xin et al. "A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest" . | OPEN BIOLOGY 15 . 4 (2025) . |
| APA | Miao, Xin , Cao, Fang , Yu, Xiao-Fei , Li, Tian-Pu , Su, Hai-Yin , Guo, Jiao et al. A zinc finger protein shapes the temperature adaptability of a cosmopolitan pest . | OPEN BIOLOGY , 2025 , 15 (4) . |
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The insect-resistant rice variety mfb-MH3301-1, expressing Cry1Ab, has demonstrated robust resistance against Lepidopteran pests. However, its potential effects on non-target organisms warrant further investigation. This study evaluated the effects of mfb-MH3301-1 in comparison with the parental rice variety MH3301 (Oryza sativa L.) on the development of the insect pest Nilaparvata lugens (St & aring;l) (Homoptera: Delphacidae). The results showed that there were no significant differences in female longevity, male longevity, lifespan, and mean generation time (T) between brown planthoppers fed on either mfb-MH3301-1 or MH3301. However, N. lugens individuals fed on mfb-MH3301-1 exhibited significantly higher fecundity, intrinsic rate of increase (r), finite rate of increase (lambda), and net reproductive rate (R0) compared to those fed on MH3301. The study provides valuable insight into the management of N. lugens in the future cultivation of Bt rice, and attention should be paid to the population dynamics of N. lugens in the field.
Keyword ：
age-stage two-sex life table age-stage two-sex life table brown planthopper brown planthopper Bt rice Bt rice Delphacidae Delphacidae Homoptera Homoptera insect resistance insect resistance non-target insect non-target insect pest management pest management population dynamics population dynamics transgenic rice transgenic rice
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| GB/T 7714 | Lin, Sheng , Miao, Xin , Dong, Ya-Qiang et al. Comparative demography of Nilaparvata lugens (Stål) reared on Bt rice and non-Bt rice [J]. | ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA , 2025 , 173 (8) : 869-880 . |
| MLA | Lin, Sheng et al. "Comparative demography of Nilaparvata lugens (Stål) reared on Bt rice and non-Bt rice" . | ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA 173 . 8 (2025) : 869-880 . |
| APA | Lin, Sheng , Miao, Xin , Dong, Ya-Qiang , Vasseur, Liette , You, Shi-Jun , Zhao, Xiao-Yan et al. Comparative demography of Nilaparvata lugens (Stål) reared on Bt rice and non-Bt rice . | ENTOMOLOGIA EXPERIMENTALIS ET APPLICATA , 2025 , 173 (8) , 869-880 . |
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Cannabis sativa L. has attracted increasing attention for its potential therapeutic applications, including anti- proliferative properties. In this study, we investigated the bioactivity of female flower extracts from eight cannabis varieties. The results showed significant variation in antiproliferative efficacy against three cancer cell lines (AGS, HCT116, and A549), antioxidant capacity, and total phenolic content. Two varieties, 'Galaxy CBD' and 'Mexican Sativa', were selected for widely targeted metabolomics analysis due to their contrasting anti- proliferative activity. Metabolomic analysis revealed distinct compositions of non-volatile and volatile metabolites between the two varieties, with 'Galaxy CBD' showing heightened levels of specific compounds associated with antiproliferative activity. Transcriptome analysis uncovered differential gene expression patterns across five stages of flower development, highlighting the genetic underpinnings of metabolic diversity. Integrating transcriptomic and metabolomic data revealed 'Galaxy CBD' exhibited higher expression of genes in pathways related to cannabinoid, terpenoid, and flavonoid biosynthesis, which may explain its superior antiproliferative activity. These findings provide valuable insights into the relationship between cannabis metabolite profiles, gene expression patterns and their potential medicinal properties, shedding light on the complex mechanisms underlying the antiproliferative properties of cannabis flower extracts.
Keyword ：
Antiproliferative activity Antiproliferative activity Cannabis sativa Cannabis sativa Medicinal properties Medicinal properties Metabolomics Metabolomics Transcriptomics Transcriptomics
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| GB/T 7714 | Liu, Ying , Li, Shuai , Shen, Mingwei et al. Integration of transcriptome and metabolome provides insights into metabolites and pathways associated with antiproliferative activity of cannabis flower extracts [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 223 . |
| MLA | Liu, Ying et al. "Integration of transcriptome and metabolome provides insights into metabolites and pathways associated with antiproliferative activity of cannabis flower extracts" . | INDUSTRIAL CROPS AND PRODUCTS 223 (2025) . |
| APA | Liu, Ying , Li, Shuai , Shen, Mingwei , Guo, Foqin , Li, Minxuan , Cai, Sen et al. Integration of transcriptome and metabolome provides insights into metabolites and pathways associated with antiproliferative activity of cannabis flower extracts . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 223 . |
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Climate change and intensive pesticide use impose combined stress on agricultural pests. To investigate the genetic basis of stress resilience in Plutella xylostella, we focused on PxCBR1-L, a carbonyl reductase gene highly expressed in detoxification tissues and upregulated under heat and insecticide exposure. Using clustered regularly interspaced short palindromic repeats (CRISPR) / CRISPR-associated nuclease 9, we generated PxCBR1-L knockout lines. Mutants exhibited impaired development, reduced survival, shortened lifespan, and decreased fecundity. Biochemically, knockout strains showed elevated reactive oxygen species and reduced superoxide dismutase, catalase, Carboxylesterase, glutathione S-transferase, and P450 activities, indicating compromised antioxidant and detoxification functions. Heat-stressed mutants showed lower critical thermal maximum, reduced survival, and increased insecticide sensitivity. Moreover, mutants exhibited significantly higher supercooling and freezing points, and elevated water loss and mortality under desiccation stress, suggesting reduced cold and drought tolerance. These findings reveal that PxCBR1-L is essential for maintaining systemic physiological homeostasis under multiple environmental stresses, and its disruption leads to broad vulnerability. This work identifies PxCBR1-L as a potential target for integrated pest management in a changing climate.
Keyword ：
carbonyl reductase carbonyl reductase CRISPR/Cas9 CRISPR/Cas9 physiological homeostasis physiological homeostasis Plutella xylostella Plutella xylostella stress tolerance stress tolerance
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| GB/T 7714 | Miao, Xin , Zhang, Yu , Chen, Bingwei et al. Functional characterization of PxCBR1-L reveals its essential role in multi-stress tolerance in a cosmopolitan pest [J]. | INSECT SCIENCE , 2025 . |
| MLA | Miao, Xin et al. "Functional characterization of PxCBR1-L reveals its essential role in multi-stress tolerance in a cosmopolitan pest" . | INSECT SCIENCE (2025) . |
| APA | Miao, Xin , Zhang, Yu , Chen, Bingwei , Ma, Zongyao , Su, Haiyin , Li, Bingxue et al. Functional characterization of PxCBR1-L reveals its essential role in multi-stress tolerance in a cosmopolitan pest . | INSECT SCIENCE , 2025 . |
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Understanding how plants respond to environmental stressors such as ultraviolet B (UV-B) radiation is critical for agricultural resilience and crop improvement. Investigation of the UV-B response in 17 cannabis accessions revealed distinct differences in morphological changes between two groups, A and B. Whole-genome resequencing and population-level analyses indicate that Delta 9-tetrahydrocannabinolic acid synthase (THCAS) is subject to positive selection or a selective sweep. Sequence analysis and functional characterization of THCAS revealed that two single nucleotide variations, resulting in two amino acids change at Pro333 and Ser355, which are associated with THCAS activity and potentially linked to differential plant response to UV-B radiation. The role of THCAS in enhancing UV-B tolerance was further demonstrated by overexpressing functional THCAS in different plant species under UV-B stress environment. This study suggests that THCA, produced by the enzyme THCAS, acts as a shield against UV-B radiation. Plants with higher levels of THCA exhibited greater tolerance to UV-B exposure. Exogenous application of THCA alleviated UV-B damage in various plant species. Our findings highlight the importance of THCAS-mediated THCA synthesis in conferring UV-B tolerance and provide insights into the genomic and molecular mechanisms that govern plant adaptation to UV-B radiation.
Keyword ：
adaptation adaptation cannabinoids cannabinoids Cannabis sativa (Cannabis) Cannabis sativa (Cannabis) THCAS THCAS UV-B radiation UV-B radiation
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| GB/T 7714 | Li, Minxuan , Ouyang, Yuzeng , Cai, Sen et al. A New UV-B Protectant in Plant: Insights Into THCAS and THCA's Role in UV-B Tolerance [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (10) : 7729-7742 . |
| MLA | Li, Minxuan et al. "A New UV-B Protectant in Plant: Insights Into THCAS and THCA's Role in UV-B Tolerance" . | PLANT CELL AND ENVIRONMENT 48 . 10 (2025) : 7729-7742 . |
| APA | Li, Minxuan , Ouyang, Yuzeng , Cai, Sen , Zhang, Zhiyuan , Wu, Qinqin , Li, Shuai et al. A New UV-B Protectant in Plant: Insights Into THCAS and THCA's Role in UV-B Tolerance . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (10) , 7729-7742 . |
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本实用新型涉及一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯，属于实验技术领域。通过一种自制的养虫杯，由两个一次性透明航空杯叠放组合而成，装置上部分的养虫杯顶端用纱网罩住，橡皮圈扎紧，作为透气口；底端中间位置钻孔，插入茶树嫩梢作为食源。装置下部分的养虫杯中盛2/3水，用脱脂棉包裹住茶枝与杯孔的缝隙处，防止叶蝉逃逸。本实用新型简单实用，价格低廉，体积轻巧，大大提高了观察效率的同时，减轻了饲养期间的工作量，便于重复性多，需虫量大的室内毒力测定实验。非常适合茶小绿叶蝉的室内饲养和科研用途研究，且可为其他昆虫的大规模饲养提供借鉴。
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| GB/T 7714 | 周敏 , 尤士骏 , 李金玉 et al. 一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯 : CN202323295506.3[P]. | 2023-12-04 . |
| MLA | 周敏 et al. "一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯" : CN202323295506.3. | 2023-12-04 . |
| APA | 周敏 , 尤士骏 , 李金玉 , 尤民生 . 一种用于茶小绿叶蝉室内饲养、毒力测定及行为观察的养虫杯 : CN202323295506.3. | 2023-12-04 . |
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The fluctuation in temperature poses a significant challenge for poikilothermic organisms, notably insects, particularly in the context of changing climatic conditions. In insects, temperature adaptation has been driven by polygenes. In addition to genes that directly affect traits (core genes), other genes (peripheral genes) may also play a role in insect temperature adaptation. This study focuses on two peripheral genes, the GRIP and coiled-coil domain containing 2 (GCC2) and karyopherin subunit beta 1 (KPNB1). These genes are differentially expressed at different temperatures in the cosmopolitan pest, Plutella xylostella. GCC2 and KPNB1 in P. xylostella were cloned, and their relative expression patterns were identified. Reduced capacity for thermal adaptation (development, reproduction and response to temperature extremes) in the GCC2-deficient and KPNB1-deficient P. xylostella strains, which were constructed by CRISPR/Cas9 technique. Deletion of the PxGCC2 or PxKPNB1 genes in P. xylostella also had a differential effect on gene expression for many traits including stress resistance, resistance to pesticides, involved in immunity, trehalose metabolism, fatty acid metabolism and so forth. The ability of the moth to adapt to temperature via different pathways is likely to be key to its ability to remain an important pest species under predicted climate change conditions.
Keyword ：
CRISPR/Cas9 system CRISPR/Cas9 system peripheral genes peripheral genes Plutella xylostella Plutella xylostella regulatory network regulatory network temperature adaptability temperature adaptability
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| GB/T 7714 | Lei, Gaoke , Huang, Jieling , Zhou, Huiling et al. Polygenic adaptation of a cosmopolitan pest to a novel thermal environment [J]. | INSECT MOLECULAR BIOLOGY , 2024 , 33 (4) : 387-404 . |
| MLA | Lei, Gaoke et al. "Polygenic adaptation of a cosmopolitan pest to a novel thermal environment" . | INSECT MOLECULAR BIOLOGY 33 . 4 (2024) : 387-404 . |
| APA | Lei, Gaoke , Huang, Jieling , Zhou, Huiling , Chen, Yanting , Song, Jun , Xie, Xuefeng et al. Polygenic adaptation of a cosmopolitan pest to a novel thermal environment . | INSECT MOLECULAR BIOLOGY , 2024 , 33 (4) , 387-404 . |
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