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学者姓名：黄劲飞
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Abstract ：
传统植物化学保护实践课中出现学生对农药种类繁多识别困难的畏难情绪、对农药生物测定设计性实验项目的连续失败受挫感，以及对运用植保无人机（UAV）等智慧农药施用手段仅能观摩无法动手实践抱有遗憾等问题，我们有效利用大学生的争强好胜心理，在实践教学中引入不同的挑战模式。农药种类多采用“限时分组PK式挑战”强化学生记忆；设计性实验项目耗时长采用“分段闯关式挑战”增强学生的自我肯定；高端农业智慧设备学习采用“人机大赛PK式挑战”拉大差距提高体验感。挑战模式教学法的课后问卷调查结果表明，大部分学生的实践学习内动力被充分激发出来，主动求知意识、表达交流能力及团队合作意识均得到明显提高。
Keyword ：
人机大赛 人机大赛 挑战教学 挑战教学 植物化学保护实践教学 植物化学保护实践教学 闯关挑战 闯关挑战 限时分组PK 限时分组PK
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| GB/T 7714 | 林美珍 , 顾晓军 , 黄劲飞 et al. 挑战教学模式在植物化学保护实践教学中的应用 [J]. | 智慧农业导刊 , 2025 , 5 (09) : 151-154,158 . |
| MLA | 林美珍 et al. "挑战教学模式在植物化学保护实践教学中的应用" . | 智慧农业导刊 5 . 09 (2025) : 151-154,158 . |
| APA | 林美珍 , 顾晓军 , 黄劲飞 , 肖顺 . 挑战教学模式在植物化学保护实践教学中的应用 . | 智慧农业导刊 , 2025 , 5 (09) , 151-154,158 . |
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Abstract ：
农药为保障世界粮食安全做出了巨大贡献，但也曾对人类健康及生态环境造成了一些负面影响。全社会都需要树立正确的农药观，包括正确的农药安全观、农药使用观与农药管理观3个方面。具体观点是，当前农药安全性已经达到可接受水平，但是其负面影响不会完全消失；农药的使用与管理应兼顾经济效益、社会效益和生态效益的统一。必须遵循的原则包括历史与现实分开、时代的局限与农药的缺陷分开、事实真相与认识误区分开、农药正确使用与错误使用甚至非法使用分开、科学管理与绿色壁垒分开。在此基础上，通过权衡利弊和扬长避短正确使用农药，以及稳中求进和先立后破有效管理农药，农药一定能为有效保障人类粮食安全做出更大贡献。
Keyword ：
使用 使用 农药观 农药观 安全 安全 管理 管理 综述 综述
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| GB/T 7714 | 顾晓军 , 林美珍 , 兰亦全 et al. 浅议如何树立正确的农药观 [J]. | 现代农药 , 2025 , 24 (03) : 50-54 . |
| MLA | 顾晓军 et al. "浅议如何树立正确的农药观" . | 现代农药 24 . 03 (2025) : 50-54 . |
| APA | 顾晓军 , 林美珍 , 兰亦全 , 黄劲飞 , 田素芬 . 浅议如何树立正确的农药观 . | 现代农药 , 2025 , 24 (03) , 50-54 . |
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Targeting juvenile hormone esterase (JHE) is an emerging strategy to combat the broadly resistant pest, Plutella xylostella; this study employed transcriptomics to investigate the sublethal effects of the JHE inhibitor OTFP, revealing a non-monotonic dose response characterized by stronger transcriptional changes at lower concentrations, resulting in low mortality, prolonged pupation time, and increased pupal weight. The results from the Differentially Expressed Genes (DEGs) analysis revealed that the core effect of OTFP is the persistent perturbation of the "insect hormone biosynthesis" pathway and altered expression of components of the JH/20E axis; to cope with this stress, the larvae exhibited a dual defense associated with compensatory upregulation of JH-degrading enzyme genes to attempt to restore hormone homeostasis, and the activation of a broad-spectrum detoxification network to clear the compound. More critically, the developmental delay resulting from endocrine disruption KEGG-enriched growth-related pathways (amino-acid and central-carbon metabolism; ribosome biogenesis; aminoacyl-tRNA biosynthesis), consistent with a growth-permissive milieu during prolonged feeding. This study therefore elucidates a novel integrative regulatory network that links endocrine disruption, detoxification, and compensatory growth, revealing a complex physiological trade-off strategy in this pest that sacrifices developmental tempo for survival.
Keyword ：
insect hormone pathway insect hormone pathway juvenile hormone esterase juvenile hormone esterase OTFP OTFP Plutella xylostella Plutella xylostella RNA-seq RNA-seq
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| GB/T 7714 | Wang, Yingbo , Wang, Xiying , Lin, Yubin et al. Transcriptomic Analysis of the Diamondback Moth Under Exposure to the Juvenile Hormone Esterase (JHE) Inhibitor 3-Octylthio-1,1,1-trifluoro-2-propanone (OTFP) [J]. | INSECTS , 2025 , 16 (11) . |
| MLA | Wang, Yingbo et al. "Transcriptomic Analysis of the Diamondback Moth Under Exposure to the Juvenile Hormone Esterase (JHE) Inhibitor 3-Octylthio-1,1,1-trifluoro-2-propanone (OTFP)" . | INSECTS 16 . 11 (2025) . |
| APA | Wang, Yingbo , Wang, Xiying , Lin, Yubin , Zheng, Shun , Qiu, Jianrong , Gao, Jinheng et al. Transcriptomic Analysis of the Diamondback Moth Under Exposure to the Juvenile Hormone Esterase (JHE) Inhibitor 3-Octylthio-1,1,1-trifluoro-2-propanone (OTFP) . | INSECTS , 2025 , 16 (11) . |
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Sulfamethoxazole (SMX) is widely employed as an antibiotic, while its residue in environment has become a common public concern. Using 100 mg/L SMX as the sole nutrient source, the acclimated sludge obtained by this study displayed an excellent SMX degradation performance. The addition of SMX resulted in significant microbiological differentiation within the acclimated sludge. Microbacterium (6.6%) was identified as the relatively dominant genera in metabolism group that used SMX as sole carbon source. Highly expressed proteins from this strain strongly suggested its essential role in SMX degradation, while the degradation of SMX by other strains (Thaurea 78%) in co -metabolism group appeared to also rely on this strain. The interactions of differentially expressed proteins were primarily involved in metabolic pathways including TCA cycle and nitrogen metabolism. It is concluded that the sulfonamides might serve not only as the carbon source but also as the nitrogen source in the reactor. A total of 24 intermediates were identified, 13 intermediates were newly reported. The constructed pathway suggested the mineralizing and nitrogen conversion ability towards SMX. Batch experiments also proved that the acclimated sludge displayed ability to biodegrade other sulfonamides, including SM2 and SDZ and SMX-N could be removed completely.
Keyword ：
Biodegradation Biodegradation Microbial consortia Microbial consortia Nitrogen removal Nitrogen removal Proteomics Proteomics Sulfamethoxazole Sulfamethoxazole
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| GB/T 7714 | He, Yuzhe , Liu, Lei , Wang, Qi et al. Bio-degraded of sulfamethoxazole by microbial consortia without addition nutrients: Mineralization, nitrogen removal, and proteomic characterization [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2024 , 466 . |
| MLA | He, Yuzhe et al. "Bio-degraded of sulfamethoxazole by microbial consortia without addition nutrients: Mineralization, nitrogen removal, and proteomic characterization" . | JOURNAL OF HAZARDOUS MATERIALS 466 (2024) . |
| APA | He, Yuzhe , Liu, Lei , Wang, Qi , Dong, Xiaoqi , Huang, Jingfei , Jia, Xiaoshan et al. Bio-degraded of sulfamethoxazole by microbial consortia without addition nutrients: Mineralization, nitrogen removal, and proteomic characterization . | JOURNAL OF HAZARDOUS MATERIALS , 2024 , 466 . |
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The coupling of modified nanoscale zero-valent iron (nZVI) with organohalide-degrading bacteria provides a promising solution for the remediation of hexabromocyclododecane (HBCD)-contaminated environments. However, the interactions between modified nZVI and dehalogenase bacteria are intricate, and the mechanisms of synergistic action and electron transfer are not clear, and requires further specific investigation. In this study, HBCD was used as a model pollutant, and stable isotope analysis revealed that organic montmorillonite (OMt)-supported nZVI coupled with the degrading bacterial strain Citrobacter sp. Y3 (nZVI/OMt–Y3) can use [13C]HBCD as the sole carbon source and degrade or even mineralise it into 13CO2 with a maximum conversion rate of 100% within approximately 5 days. Analysis of the intermediates showed that the degradation of HBCD mainly involves three different pathways: dehydrobromination, hydroxylation, and debromination. The proteomics results showed that nZVI introduction promoted the transport of electrons and debromination. Combining the results from XPS, FTIR, and Raman spectroscopy with the analysis results of proteinomics and biodegradation products, we verified the process of electron transport and proposed a metabolic mechanism of HBCD degradation by the nZVI/OMt–Y3. Moreover, this study provides insightful avenues and models for the further remediation of HBCD and other similar pollutants in the environment. © 2023, The Authors. All rights reserved.
Keyword ：
Bacteria Bacteria Biodegradation Biodegradation Clay minerals Clay minerals Electron transitions Electron transitions Electron transport properties Electron transport properties Fourier transform infrared spectroscopy Fourier transform infrared spectroscopy Iron Iron Metabolism Metabolism Molecular biology Molecular biology
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| GB/T 7714 | Li, Tianyu , Lu, Yingyuan , Liu, Lei et al. Efficient Degradation of Hexabromocyclododecane Using Montmorillonite Supported Nano-Zero-Valent Iron and Citrobacter Sp. Y3 [J]. | SSRN , 2023 . |
| MLA | Li, Tianyu et al. "Efficient Degradation of Hexabromocyclododecane Using Montmorillonite Supported Nano-Zero-Valent Iron and Citrobacter Sp. Y3" . | SSRN (2023) . |
| APA | Li, Tianyu , Lu, Yingyuan , Liu, Lei , He, Yuzhe , Huang, Jingfei , Peng, Xingxing . Efficient Degradation of Hexabromocyclododecane Using Montmorillonite Supported Nano-Zero-Valent Iron and Citrobacter Sp. Y3 . | SSRN , 2023 . |
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Abstract ：
The coupling of modified nanoscale zero-valent iron (nZVI) with organohalide-degrading bacteria provides a promising solution for the remediation of hexabromocyclododecane (HBCD)-contaminated environments. However, the interactions between modified nZVI and dehalogenase bacteria are intricate, and the mechanisms of synergistic action and electron transfer are not clear, and requires further specific investigation. In this study, HBCD was used as a model pollutant, and stable isotope analysis revealed that organic montmorillonite (OMt)-supported nZVI coupled with the degrading bacterial strain Citrobacter sp. Y3 (nZVI/OMt-Y3) can use [13C]HBCD as the sole carbon source and degrade or even mineralise it into 13CO2 with a maximum conversion rate of 100% within approximately 5 days. Analysis of the intermediates showed that the degradation of HBCD mainly involves three different pathways: dehydrobromination, hydroxylation, and debromination. The proteomics results showed that nZVI introduction promoted the transport of electrons and debromination. Combining the results from XPS, FTIR, and Raman spec-troscopy with the analysis results of proteinomics and biodegradation products, we verified the process of electron transport and proposed a metabolic mechanism of HBCD degradation by the nZVI/OMt-Y3. Moreover, this study provides insightful avenues and models for the further remediation of HBCD and other similar pollutants in the environment.
Keyword ：
Electron transfer Electron transfer Hexabromocyclododecane Hexabromocyclododecane Metabolic mechanism Metabolic mechanism Mineralisation Mineralisation NZVI NZVI OMt-Y3 OMt-Y3
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| GB/T 7714 | Li, Tianyu , Lu, Yingyuan , Liu, Lei et al. Efficient degradation of hexabromocyclododecane using montmorillonite supported nano-zero-valent iron and Citrobacter sp. Y3 [J]. | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 457 . |
| MLA | Li, Tianyu et al. "Efficient degradation of hexabromocyclododecane using montmorillonite supported nano-zero-valent iron and Citrobacter sp. Y3" . | JOURNAL OF HAZARDOUS MATERIALS 457 (2023) . |
| APA | Li, Tianyu , Lu, Yingyuan , Liu, Lei , He, Yuzhe , Huang, Jingfei , Peng, Xingxing . Efficient degradation of hexabromocyclododecane using montmorillonite supported nano-zero-valent iron and Citrobacter sp. Y3 . | JOURNAL OF HAZARDOUS MATERIALS , 2023 , 457 . |
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Candida tropicalis PNY, a novel dimorphic strain with the capacity of simultaneous carbon, nitrogen and phosphorus removal in anaerobic and aerobic conditions, was isolated from activated sludge. Dimorphism of C. tropicalis PNY had effect on removing nitrogen and phosphorous and slightly affected COD removal under aerobic condition. Sample with high hypha formation rate (40 +/- 5%) had more removal efficiencies of NH4+-N (50 mg/L) and PO43- -P (10 mg/L), which could achieve 82.19% and 97.53%, respectively. High hypha cells dosage exhibited good settleability and filamentous overgrowth was not observed. According to label-free quantitative proteomics assays. Up-regulated proteins involved in the mitogen-activated protein kinase (MAPK) pathway indicated the active growth and metabolism process of sample with high hypha formation rate (40 +/- 5%). And proteins concerning about glutamate synthetase and SPX domain-contain protein explain for the nutrient removal mechanism including assimilation of ammonia and polyphosphates synthesis.
Keyword ：
Candida tropicalis Candida tropicalis Carbon Carbon Dimorphism Dimorphism Nitrogen and phosphorous removal Nitrogen and phosphorous removal Proteomic response Proteomic response Response surface methodology (RSM) Response surface methodology (RSM)
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| GB/T 7714 | Zhang, Yaqi , He, Yuzhe , Huang, Jingfei et al. Dimorphism of Candida tropicalis and its effect on nitrogen and phosphorus removal and sludge settleability [J]. | BIORESOURCE TECHNOLOGY , 2023 , 382 . |
| MLA | Zhang, Yaqi et al. "Dimorphism of Candida tropicalis and its effect on nitrogen and phosphorus removal and sludge settleability" . | BIORESOURCE TECHNOLOGY 382 (2023) . |
| APA | Zhang, Yaqi , He, Yuzhe , Huang, Jingfei , Chen, Jiejing , Jia, Xiaoshan , Peng, Xingxing . Dimorphism of Candida tropicalis and its effect on nitrogen and phosphorus removal and sludge settleability . | BIORESOURCE TECHNOLOGY , 2023 , 382 . |
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【目的】本研究旨在为阐明小菜蛾Plutella xylostella体温在其防治中的应用价值提供资料。【方法】在不同人工气候箱内温度(环境温度)下，测定小菜蛾2, 3和4龄幼虫的体温，建立各龄幼虫体温(y)与环境温度(x)的关系方程；同时测定了不同环境温度下不同浓度阿维菌素、毒死蜱、氟虫腈和高效氯氰菊酯分别处理后小菜蛾3龄幼虫在不同处理时间的体温。【结果】小菜蛾2, 3和4龄幼虫体温(y)与环境温度(x)关系方程分别为y=0.95x+1.19(r=0.9463),y=0.95x+1.18(r=0.9988),以及y=0.93x+1.45(r=0.9989),等温点分别为22.16℃, 21.40℃和21.41℃。在气候箱温度设定为15℃或40℃时，4种农药都对小菜蛾3龄幼虫体温无影响；而其他温度条件下，农药处理都可能改变小菜蛾3龄幼虫体温。对于阿维菌素，25℃下2, 4和8 mg/L处理12 h, 2和4 mg/L处理24 h, 0.5, 2, 4和8 mg/L处理36 h以及0.5, 1, 2和8 mg/L处理48 h时3龄幼虫体温均显著高于对照，8 mg/L阿维菌素处理24 h时3龄幼虫体温显著低于对照；30℃下0.5 mg/L处理24 h及1 mg/L处理36 h 3龄幼虫体温显著低于对照，1 mg/L处理48 h和各浓度处理60 h时3龄幼虫体温均显著高于对照；35℃下只有1和8 mg/L处理48 h时3龄幼虫体温显著低于对照。对于毒死蜱，20℃下50, 200和800 mg/L处理24 h, 100, 400和800 mg/L处理36 h时3龄幼虫体温都显著低于对照；25℃下100和200 mg/L处理12 h, 800 mg/L处理24 h, 100, 200和800 mg/L处理60 h时3龄幼虫体温均显著低于对照，而50, 100, 200和400 mg/L处理24 h, 100和200 mg/L处理36 h及100和400 mg/L处理48 h时3龄幼虫体温均显著高于对照；30℃下只有800 mg/L处理24 h时3龄幼虫体温显著低于对照，50, 100, 200和800 mg/L处理60 h时3龄幼虫体温显著高于对照。对于氟虫腈，20℃下只有0.5 mg/L处理36 h时3龄幼虫体温显著低于对照；25℃下4 mg/L处理12 h和各浓度处理60 h时3龄幼虫体温显著低于对照，0.5 mg/L处理24 h以及0.25, 1和2 mg/L处理48 h时3龄幼虫体温均显著高于对照；30℃下0.25和0.5 mg/L处理12 h, 0.25和2 mg/L处理24 h, 4 mg/L处理48 h以及2 mg/L处理60 h时3龄幼虫体温显著低于对照；35℃下只有0.25和0.5 mg/L处理60 h时3龄幼虫体温显著高于对照。对于高效氯氰菊酯，20℃下2和8 g/L处理36 h, 4和8 g/L处理48 h时3龄幼虫体温显著高于对照；25℃下2, 4和8 g/L处理12 h时3龄幼虫体温均显著低于对照，0.5, 4和8 g/L处理24 h, 1, 4和8 g/L处理36 h以及1, 2和4 g/L处理60 h时3龄幼虫体温均显著高于对照；30℃下0.5和1 g/L浓度处理12 h, 0.5, 1, 4和8 g/L处理24 h以及1, 2和8 g/L处理60 h时3龄幼虫体温都显著低于对照。【结论】小菜蛾幼虫自律性体温调节能力低；阿维菌素、毒死蜱、氟虫腈或高效氯氰菊酯处理影响小菜蛾3龄幼虫的体温，影响形式随农药种类和浓度，环境温度及处理时间不同而不同。本研究拓宽了农药毒理学及害虫防治研究内容。
Keyword ：
体温 体温 农药 农药 小菜蛾 小菜蛾 毒力 毒力 等温点 等温点
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| GB/T 7714 | 田素芬 , 李志华 , 李元涛 et al. 四种农药对小菜蛾体温的影响 [J]. | 昆虫学报 , 2022 , 65 (09) : 1115-1126 . |
| MLA | 田素芬 et al. "四种农药对小菜蛾体温的影响" . | 昆虫学报 65 . 09 (2022) : 1115-1126 . |
| APA | 田素芬 , 李志华 , 李元涛 , 林硕 , 陈艺欣 , 田厚军 et al. 四种农药对小菜蛾体温的影响 . | 昆虫学报 , 2022 , 65 (09) , 1115-1126 . |
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为探讨生长阻滞和DNA损伤诱导基因Gadd45g对小菜蛾Plutella xylostella生长发育的影响及其对蜕皮激素类似物的响应,利用全长cDNA末端的快速克隆(rapid amplification of cDNA ends,RACE)技术克隆小菜蛾Gadd45g的全长序列并进行生物信息学分析,采用实时荧光定量PCR技术分析不同龄期及不同浓度虫酰肼处理后Gadd45g基因的表达水平;同时利用dsRNA注射法干扰蛹期小菜蛾Gadd45g基因,分析该基因对蛹期特异基因Br-Z2/3表达量及小菜蛾羽化进程的影响。结果显示,小菜蛾Gadd45g基因序列全长为1 479 bp,其中编码区序列长度为495 bp,可编码164个氨基酸,GenBank登录号为MW160738。小菜蛾GADD45γ蛋白与鳞翅目昆虫的GADD45同源性最高,与脊椎动物的同源性较低。Gadd45g基因在小菜蛾全龄期均有表达,其中在蛹期的相对表达量最高,显著高于其他龄期,并在蛹期和成虫期具有明显的性别表达差异;低剂量虫酰肼处理后小菜蛾Gadd45g基因相对表达量显著上升。干扰Gadd45g基因表达后小菜蛾蛹期特异基因Br-Z2/3的表达量及小菜蛾羽化率均显著降低,并且成虫出现畸形。表明GADD45γ能响应蜕皮激素类似物,在小菜蛾蛹期发育及羽化过程中起重要作用。
Keyword ：
Gadd45g Gadd45g RNA干扰 RNA干扰 小菜蛾 小菜蛾 显微注射 显微注射 表达谱 表达谱
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| GB/T 7714 | 胡晓汉 , 刘向 , 黄劲飞 . 小菜蛾Gadd45g基因的克隆、表达谱及RNA干扰对小菜蛾羽化过程的影响 [J]. | 植物保护学报 , 2022 , 49 (04) : 1132-1142 . |
| MLA | 胡晓汉 et al. "小菜蛾Gadd45g基因的克隆、表达谱及RNA干扰对小菜蛾羽化过程的影响" . | 植物保护学报 49 . 04 (2022) : 1132-1142 . |
| APA | 胡晓汉 , 刘向 , 黄劲飞 . 小菜蛾Gadd45g基因的克隆、表达谱及RNA干扰对小菜蛾羽化过程的影响 . | 植物保护学报 , 2022 , 49 (04) , 1132-1142 . |
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【目的】本研究旨在构建用于小菜蛾Plutella xylostella蛹期特异表达基因Br-Z2/3 dsRNA合成的体外原核表达系统,研究RNAi抑制Br-Z2/3基因对小菜蛾Br-Z2/3和细胞凋亡基因表达及化蛹的影响。【方法】构建小菜蛾L4440-Br-Z2/3重组载体,转化大肠杆菌Escherichia coli HT115感受态细胞,经IPTG诱导大量获得Br-Z2/3 dsRNA,显微注射Br-Z2/3 dsRNA至小菜蛾4龄幼虫进行RNAi; qPCR检测干扰Br-Z2/3基因12和24 h后小菜蛾4龄幼虫Br-Z2/3及其下游细胞凋亡基因reaper,caspase-9和Gadd45g的表达量;观察并统计Br-Z2/3 RNAi后小菜蛾4龄幼虫的化蛹率、平均化蛹时间、蛹畸形率和幼虫死亡率。【结果】成功实现了Br-Z2/3 dsRNA的原核表达。qPCR结果表明,RNAi干扰Br-Z2/3后,小菜蛾4龄幼虫Br-Z2/3基因和相关联的细胞凋亡基因reaper表达量显著下降,但caspase-9和Gadd45g表达量显著上升。注射Br-Z2/3 dsRNA的处理组,化蛹率显著低于对照组,且化蛹高峰期推迟,幼虫死亡率显著高于对照组且畸形蛹率增加。【结论】本研究成功构建了用于小菜蛾Br-Z2/3基因dsRNA合成的体外原核表达系统,利用显微注射法对Br-Z2/3进行RNA干扰,证明Br-Z2/3是调控小菜蛾化蛹的关键基因。
Keyword ：
Br-Z2/3 Br-Z2/3 dsRNA dsRNA RNA干扰 RNA干扰 化蛹 化蛹 小菜蛾 小菜蛾 显微注射 显微注射 细胞凋亡基因 细胞凋亡基因
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| GB/T 7714 | 胡晓汉 , 田素芬 , 李亚勍 et al. RNAi抑制小菜蛾Br-Z2/3基因对下游细胞凋亡基因表达及化蛹的影响 [J]. | 昆虫学报 , 2021 , 64 (02) : 141-148 . |
| MLA | 胡晓汉 et al. "RNAi抑制小菜蛾Br-Z2/3基因对下游细胞凋亡基因表达及化蛹的影响" . | 昆虫学报 64 . 02 (2021) : 141-148 . |
| APA | 胡晓汉 , 田素芬 , 李亚勍 , 林硕 , 陈艺欣 , 魏辉 et al. RNAi抑制小菜蛾Br-Z2/3基因对下游细胞凋亡基因表达及化蛹的影响 . | 昆虫学报 , 2021 , 64 (02) , 141-148 . |
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