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学者姓名：吕明发
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Abstract ：
Bacterial soft rot, mainly caused by Dickeya and Pectobacterium pathogens, leads to severe economic losses in agriculture. Recently, we isolated a rhizosphere strain Pseudomonas aeruginosa Y010, which is a potential biocontrol agent for bacterial soft rot. However, the underlying antagonistic mechanisms remain unknown. In this study, we explored the antagonistic mechanism of P. aeruginosa Y010 against two important pathogens, P. aroidearum ZL1 and D. fangzhongdai CL3, and found that pyocyanin (PYO) is the key component exhibiting antagonistic activity. Using the green-blue color PYO production as a rapid readout, genome-wide transposon mutagenesis successfully generated a mutant (26330::Tn) with elevated PYO production and biocontrol efficacy. Both Y010 and its genetically modified mutant 26330::Tn exhibited no phytotoxicity to plants, highlighting their biosafety as biocontrol agents. This study elucidates the antagonistic mechanism of Y010 and provides a strategy of rapid genetic modification for biocontrol agents to manage plant diseases.
Keyword ：
biocontrol biocontrol Pseudomonasaeruginosa Pseudomonasaeruginosa pyocyanin pyocyanin secondary metabolites secondary metabolites soft rot soft rot
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| GB/T 7714 | Wen, Yongqi , Pu, Xiaoming , Lv, Mingfa et al. Unveiling the Antagonistic Mechanism of Pseudomonas aeruginosa Y010 Directs Targeted Genetic Screening for Enhanced Biocontrol Efficacy [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (28) : 17685-17695 . |
| MLA | Wen, Yongqi et al. "Unveiling the Antagonistic Mechanism of Pseudomonas aeruginosa Y010 Directs Targeted Genetic Screening for Enhanced Biocontrol Efficacy" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 28 (2025) : 17685-17695 . |
| APA | Wen, Yongqi , Pu, Xiaoming , Lv, Mingfa , Feng, Ruqing , Yang, Sheng , Gao, Xiyue et al. Unveiling the Antagonistic Mechanism of Pseudomonas aeruginosa Y010 Directs Targeted Genetic Screening for Enhanced Biocontrol Efficacy . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (28) , 17685-17695 . |
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Abstract ：
Virulence factor modulating (VFM) is a quorum sensing (QS) signal shared by and specific to Dickeya bacteria, regulating the production of plant cell wall degrading enzymes (PCWDEs) and virulence of Dickeya. High polarity and trace of VFM signal increase the difficulty of signal separation and structure identification, and thus limit the development of quorum quenching strategy to biocontrol bacterial soft rot diseases caused by Dickeya. In order to high-throughput screen VFM quenching bacteria, a vfmE-gfp biosensor VR2 (VFM Reporter) sensitive to VFM signal was first constructed. Subsequently, two bacterial strains with high quenching efficiency were screened out by fluorescence intensity measurement and identified as Pseudomonas chlororaphis L5 and Enterobacter asburiae L95 using multilocus sequence analysis (MLSA). L5 and L95 supernatants reduced the expression of vfm genes, and both strains also decreased the production of PCWDEs of D. zeae MS2 and significantly reduced the virulence of D. oryzae EC1 on rice seedlings, D. zeae MS2 on banana seedlings, D. dadantii 3937 on potato and D. fangzhongdai CL3 on taro. Findings in this study provide a method to high-throughput screen VFM quenching bacteria and characterize novel functions of P. chlororaphis and E. asburiae in biocontrolling plant diseases through quenching VFM QS signal.
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| GB/T 7714 | Liu, Fan , Hu, Ming , Tan, Xu et al. Pseudomonas chlororaphis L5 and Enterobacter asburiae L95 biocontrol Dickeya soft rot diseases by quenching virulence factor modulating quorum sensing signal [J]. | MICROBIAL BIOTECHNOLOGY , 2023 , 16 (11) : 2145-2160 . |
| MLA | Liu, Fan et al. "Pseudomonas chlororaphis L5 and Enterobacter asburiae L95 biocontrol Dickeya soft rot diseases by quenching virulence factor modulating quorum sensing signal" . | MICROBIAL BIOTECHNOLOGY 16 . 11 (2023) : 2145-2160 . |
| APA | Liu, Fan , Hu, Ming , Tan, Xu , Xue, Yang , Li, Chuhao , Wang, Si et al. Pseudomonas chlororaphis L5 and Enterobacter asburiae L95 biocontrol Dickeya soft rot diseases by quenching virulence factor modulating quorum sensing signal . | MICROBIAL BIOTECHNOLOGY , 2023 , 16 (11) , 2145-2160 . |
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Abstract ：
The zeamines produced by Dickeya oryzae are potent polyamine antibiotics and phytotoxins that are essential for bacterial virulence. We recently showed that the RND efflux pump DesABC in D. oryzae confers partial resistance to zeamines. To fully elucidate the bacterial self-protection mechanisms, in this study we used transposon mutagenesis to identify the genes encoding proteins involved in zeamine resistance in D. oryzae EC1. This led to the identification of a seven-gene operon, arn(EC1), that encodes enzyme homologues associated with lipopolysaccharide modification. Deletion of the arn(EC1) genes in strain EC1 compromised its zeamine resistance 8- to 16-fold. Further deletion of the des gene in the arn(EC1) mutant background reduced zeamine resistance to a level similar to that of the zeamine-sensitive Escherichia coli DH5a. Intriguingly, the arn(EC1) mutants showed varied bacterial virulence on rice, potato, and Chinese cabbage. Further analyses demonstrated that ArnBCAT(EC1) are involved in maintenance of the bacterial nonmucoid morphotype by repressing the expression of capsular polysaccharide genes and that ArnB(EC1) is a bacterial virulence determinant, influencing transcriptional expression of over 650 genes and playing a key role in modulating bacterial motility and virulence. Taken together, these findings decipher a novel zeamine resistance mechanism in D. oryzae and document new roles of the Arn enzymes in modulation of bacterial physiology and virulence.
Keyword ：
antimicrobial resistance antimicrobial resistance arn operon arn operon cell division cell division lipopolysaccharide lipopolysaccharide motility motility
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| GB/T 7714 | Liang, Zhibin , Huang, Luhao , Liu, Huidi et al. Characterization of the Arn lipopolysaccharide modification system essential for zeamine resistance unveils its new roles in Dickeya oryzae physiology and virulence [J]. | MOLECULAR PLANT PATHOLOGY , 2023 , 24 (12) : 1480-1494 . |
| MLA | Liang, Zhibin et al. "Characterization of the Arn lipopolysaccharide modification system essential for zeamine resistance unveils its new roles in Dickeya oryzae physiology and virulence" . | MOLECULAR PLANT PATHOLOGY 24 . 12 (2023) : 1480-1494 . |
| APA | Liang, Zhibin , Huang, Luhao , Liu, Huidi , Zheng, Ying , Feng, Jiani , Shi, Zurong et al. Characterization of the Arn lipopolysaccharide modification system essential for zeamine resistance unveils its new roles in Dickeya oryzae physiology and virulence . | MOLECULAR PLANT PATHOLOGY , 2023 , 24 (12) , 1480-1494 . |
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Abstract ：
Phytopathogen Dickeya oryzae is a causal agent of rice foot rot disease and the pathogen has an array of virulence factors, such as phytotoxin zeamines, plant cell wall degrading enzymes, cell motility, and biofilms, collectively contributing to the bacterial pathogenesis. In this study, through deletion analysis of predicted regulatory genes in D. oryzae EC1, we identified a two-component system associated with the regulation of bacterial virulence. The two-component system contains a histidine kinase ArcB and a response regulator ArcA, and deletion of their coding genes resulted in changed phenotypes in cell motility, biofilm formation, and bacterial virulence. Electrophoretic mobility shift assay revealed that ArcA bound to the promoters of the bcs operon and bssS, which respectively encode enzymes for the synthesis of celluloses and a biofilm formation regulatory protein. ArcA could also bind to the promoters of three virulence associated transcriptional regulatory genes, i.e., fis, slyA and ohrR. Surprisingly, although these three regulators were shown to modulate the production of cell wall degrading enzymes and zeamines, deletion of arcB and arcA did not seem to affect these phenotypes. Taken together, the findings from this study unveiled a new two-component system associated with the bacterial pathogenesis, which contributes to the virulence of D. oryzae mainly through its action on bacterial motility and biofilm formation.
Keyword ：
biofilm biofilm Dickeya oryzae Dickeya oryzae motility motility two-component system two-component system virulence virulence
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| GB/T 7714 | Lv, Mingfa , Ye, Sixuan , Hu, Ming et al. Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae [J]. | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
| MLA | Lv, Mingfa et al. "Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae" . | FRONTIERS IN PLANT SCIENCE 13 (2022) . |
| APA | Lv, Mingfa , Ye, Sixuan , Hu, Ming , Xue, Yang , Liang, Zhibin , Zhou, Xiaofan et al. Two-component system ArcBA modulates cell motility and biofilm formation in Dickeya oryzae . | FRONTIERS IN PLANT SCIENCE , 2022 , 13 . |
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