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学者姓名：陈倩
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Abstract ：
唾液蛋白是刺吸式口器介体昆虫唾液的重要成分，在昆虫取食过程中调控寄主植物的防御反应、自身取食的顺利进行和病毒的有效传播。本文围绕昆虫唾液蛋白与植物防御反应的“攻-守”关系，阐述唾液蛋白作为激发子触发植物防御反应，以及作为效应子抑制植物防御反应的作用机制，论述了唾液激发子和效应子对介体昆虫取食和传播病毒的影响。介体昆虫唾液蛋白作为介导病毒传播的跨界信号，将成为制定病害调控策略的重要靶标。昆虫唾液蛋白的研究也将为开拓植物病毒病有效防控的新技术提供重要的理论基础。
Keyword ：
介体昆虫 介体昆虫 唾液蛋白 唾液蛋白 昆虫取食 昆虫取食 植物病毒 植物病毒 植物防御 植物防御
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| GB/T 7714 | 张洪祥 , 陈倩 , 魏太云 . 介体昆虫利用唾液蛋白调控植物防御促进自身取食和病毒传播的研究进展 [J]. | 生命科学 , 2025 , 37 (05) : 514-521 . |
| MLA | 张洪祥 等. "介体昆虫利用唾液蛋白调控植物防御促进自身取食和病毒传播的研究进展" . | 生命科学 37 . 05 (2025) : 514-521 . |
| APA | 张洪祥 , 陈倩 , 魏太云 . 介体昆虫利用唾液蛋白调控植物防御促进自身取食和病毒传播的研究进展 . | 生命科学 , 2025 , 37 (05) , 514-521 . |
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由水稻矮缩病毒(Rice dwarf virus, RDV)引起的水稻矮缩病是危害水稻安全生产的主要病毒病害，建立RDV的监测技术体系对于水稻矮缩病的田间早期预警与防控具有重要意义。本研究通过反转录PCR(Reverse transcription-PCR, RT-PCR)从RDV感染的水稻病株中扩增1 056 bp的P9基因片段，并将其在大肠杆菌(Escherichia coli)BL21(DE3)中表达。以纯化的P9蛋白为抗原免疫新西兰雄兔，制备P9多克隆抗血清。酶联免疫吸附测定(Enzyme-linked immunosorbent assay, ELISA)结果显示该抗血清效价为1:32 000。蛋白质印迹(Western blotting)结果表明经抗原亲和纯化获得的多克隆抗体可特异性识别RDV感染的水稻病株及带毒介体黑尾叶蝉中的RDV P9蛋白。进一步将该多克隆抗体应用于间接ELISA和斑点ELISA(dot-ELISA)分析，并结合RT-PCR对采集自福建省莆田市仙游县的田间样品，包括水稻、稗草及3种昆虫介体(黑尾叶蝉、电光叶蝉、褐飞虱)共223份样本进行检测。结果显示：相对于RDV,水稻和稗草的感病率分别为29.03%和19.05%,黑尾叶蝉、电光叶蝉和褐飞虱的带毒率分别为12.28%、2.56%和0。本研究成功制备了高效价、高特异性的RDV P9多克隆抗体，基于此建立的血清学检测方法不仅提升了水稻矮缩病诊断的可靠性，更为后续P9蛋白功能研究及RDV检测提供了技术支撑。
Keyword ：
多克隆抗体 多克隆抗体 次要外壳蛋白P9 次要外壳蛋白P9 水稻矮缩病毒(RDV) 水稻矮缩病毒(RDV)
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| GB/T 7714 | 李晶 , 张雅婷 , 林泽芬 et al. 水稻矮缩病毒次要外壳蛋白P9多克隆抗体的制备和应用 [J]. | 植物病理学报 , 2025 , 55 (04) : 991-1000 . |
| MLA | 李晶 et al. "水稻矮缩病毒次要外壳蛋白P9多克隆抗体的制备和应用" . | 植物病理学报 55 . 04 (2025) : 991-1000 . |
| APA | 李晶 , 张雅婷 , 林泽芬 , 魏太云 , 陈倩 . 水稻矮缩病毒次要外壳蛋白P9多克隆抗体的制备和应用 . | 植物病理学报 , 2025 , 55 (04) , 991-1000 . |
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Phloem-inhabiting unculturable bacterial pathogens are persistently transmitted by insect vectors. However, how they evade insect immune responses to ensure persistent transmission remains unknown. The important melanization immune response in insects is triggered by cleavage of prophenoloxidase (PPO) into active phenoloxidase (PO) via clip-domain serine proteases (CLIPs). Here, we demonstrate that infection of Candidatus Liberibacter asiaticus (CLas) in psyllid vectors activates the peptidoglycan recognition protein (PGRP)-CLIP1-CLIP4-PPO-PO signaling cascade to induce a mild melanization response, ensuring persistent CLas infection without causing significant insect fitness costs to the insect. A CLas-encoded secretory protein, SDE3230, directly interacts with PGRP and suppresses its activity in transducing this signaling cascade. CLIP4 cleaves PPO between arginine 125 and methionine 126 residues to active PO to induce melanization, and this cleavage pattern in psyllid is distinct from other insects. However, SDE3230 competitively binds to this cleavage site of PPO with CLIP4, thereby suppressing PPO effective cleavage. Collectively, these findings reveal the dual role of SDE3230 in facilitating the mild melanization response, benefitting persistent CLas infection and insect fitness.IMPORTANCEPsyllid-borne huanglongbing is the most destructive citrus disease worldwide, causing billions of dollars in annual production losses and threatening the entire citrus industry. Currently, the mechanism by which the causal agent Candidatus Liberibacter asiaticus (CLas) antagonizes psyllid innate immune responses to facilitate its coexistence with psyllid vectors is still unknown. Here, we report that CLas exploits the highly expressed secretory protein SDE3230 in psyllids to suppress the important melanization immune response in hemolymph via inhibiting the pattern recognition receptor PGRP activity and the cleavage of prophenoloxidase into active phenoloxidase by clip-domain serine proteases. The pattern of PPO cleavage is novel, and this process ultimately ensures persistent CLas infection and insect fitness. Our findings provide insights into how CLas has evolved novel strategies to evade the insect melanization response, thereby facilitating persistent CLas transmission.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus huanglongbing huanglongbing melanization melanization persistent infection persistent infection psyllid psyllid secretory protein secretory protein
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| GB/T 7714 | Li, You , Du, Yu , Ren, Dongsheng et al. Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection [J]. | MBIO , 2025 , 16 (10) . |
| MLA | Li, You et al. "Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection" . | MBIO 16 . 10 (2025) . |
| APA | Li, You , Du, Yu , Ren, Dongsheng , Bin, Yu , Chen, Qian , Wei, Taiyun . Unculturable bacteria exploit a secretory protein to antagonize insect melanization for persistent infection . | MBIO , 2025 , 16 (10) . |
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在农业生态系统中，植物病毒病流行的生物因素包括病毒、介体、寄主植物。介体昆虫、病毒、寄主植物在漫长的进化中形成复杂的互作关系。明确昆虫-病毒-植物互作的分子机制，有助于制定精准、高效的虫传病毒病控制策略。在昆虫的天然免疫和植物的防御反应过程中，自噬可降解病毒粒体，或清除被病毒侵染的细胞器，抑制病毒的侵染。为对抗来自寄主或介体昆虫的免疫攻击，有些病毒进化出操纵自噬的反防御策略，从而促进自身的侵染。本文围绕昆虫介体/植物寄主与病毒的关系，阐述昆虫/植物的细胞自噬调控病毒侵染的机制，论述自噬参与的免疫稳态在病毒侵染昆虫介体过程中的作用，为昆虫、病毒、植物三者关系提供重要的理论基础，昆虫/植物的自噬途径也可能成为控制病害传播和侵染策略的潜在靶标。
Keyword ：
昆虫 昆虫 植物 植物 病毒 病毒 细胞凋亡 细胞凋亡 细胞自噬 细胞自噬
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| GB/T 7714 | 万文强 , 魏太云 , 陈倩 . 昆虫/植物的细胞自噬调控病毒侵染的研究进展 [J]. | 应用昆虫学报 , 2025 , 62 (03) : 535-548 . |
| MLA | 万文强 et al. "昆虫/植物的细胞自噬调控病毒侵染的研究进展" . | 应用昆虫学报 62 . 03 (2025) : 535-548 . |
| APA | 万文强 , 魏太云 , 陈倩 . 昆虫/植物的细胞自噬调控病毒侵染的研究进展 . | 应用昆虫学报 , 2025 , 62 (03) , 535-548 . |
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Autophagy plays a crucial role in virus-host interactions, as viral components and particles can be degraded by the host's autophagic machinery. Additionally, some viruses can hijack autophagy for their own benefit. However, the mechanisms underlying the transcriptional regulation of autophagy by arboviruses in insect vectors remain largely unexplored. In this study, we found that rice dwarf virus (RDV) infection activates the autophagy pathway in the leafhopper vector, Nephotettix cincticeps, and this autophagy activation also facilitates viral infection in the leafhopper. We identified that MYC transcription factor regulates the expression of autophagy proteins ATG5 and ATG8 by directly targeting their promoters. A transcription regulator SMARCB1 binds to MYC and impedes its recognition of the ATG5 and ATG8 promoters, thus negatively regulating their expression. Moreover, NcSMARCB1 negatively regulates ATG5 expression by directly binding to its promoter. RDV major outer capsid protein P8 blocks the nuclear translocation of SMARCB1, disrupting the SMARCB1-MYC interaction and thereby relieving the transcriptional inhibition of ATG5 and ATG8, which leads to autophagy activation. Furthermore, major outer capsid protein P8 of rice gall dwarf virus (RGDV), same to RDV belonging to plant reoviruses, also interacts with SMARCB1 in leafhopper Recilia dorsalis, preventing its nuclear translocation. Similarly, suppression of SMARCB1 expression enhances autophagy formation and promotes RGDV infection. These findings highlight the critical role of insect vector SMARCB1 and MYC in regulating autophagy in response to arbovirus infection.
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| GB/T 7714 | Wang, Hui , Liu, Runfa , Xiao, Guangming et al. Reoviruses hijack the SMARCB1-MYC transcriptional regulation complex to activate autophagy for persistent viral infection in leafhopper vector [J]. | PLOS PATHOGENS , 2025 , 21 (10) . |
| MLA | Wang, Hui et al. "Reoviruses hijack the SMARCB1-MYC transcriptional regulation complex to activate autophagy for persistent viral infection in leafhopper vector" . | PLOS PATHOGENS 21 . 10 (2025) . |
| APA | Wang, Hui , Liu, Runfa , Xiao, Guangming , Li, Yanan , Li, Bozhong , Chen, Qian et al. Reoviruses hijack the SMARCB1-MYC transcriptional regulation complex to activate autophagy for persistent viral infection in leafhopper vector . | PLOS PATHOGENS , 2025 , 21 (10) . |
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The citrus disease Huanglongbing (HLB) in Asia and the US is caused by Candidatus Liberibacter asiaticus (CLas), which is primarily transmitted by Diaphorina citri, also known as Asian citrus psyllid in a persistent and propagative manner. However, the exact mechanisms underlying CLas circulation within D. citri remain largely unclear. Here, immunofluorescence microscopy and electron microscopy were utilized to track the sequential infection of CLas in D. citri, from alimentary canal to salivary glands, and ultimately to the plant host. CLas was found to initially infect the epithelium of filter chamber, after which it rapidly spreads to visceral muscles for further infection throughout the alimentary canal. The rapid spread in D. citri adults causes the duration of CLas circulation to be as short as 9 days. The duration of latent period may be explained by the recruitment of cytoskeletal alpha-actinin by the outer membrane protein (OMP) of CLas. Inhibition of actin filament or knocking down the expression of alpha-actinin significantly suppresses CLas cytoskeleton-dependent infection in and spread among D. citri organs. Injection of prokaryotically expressed OMP into D. citri also recruits alpha-actinin, resembling the natural infection of CLas. Our studies showed that CLas exploits alpha-actinin and remolds actin machinery of D. citri for overcoming the midgut release barrier, facilitating its circulation in the vector. By shedding light on these mechanisms, this report reveals more detailed mechanisms in CLas infection in D. citri, and offers a plausible explanation for rapid dissemination of HLB in nature from the perspective of psyllid transmission.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus Circulative infection Circulative infection Cytoskeleton Cytoskeleton Diaphorina citri Diaphorina citri
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| GB/T 7714 | Li, Zhiqiang , Yang, Xiao , Guo, Yuxin et al. Candidatus Liberibacter asiaticus exploits cytoskeletal system of psyllid vector for circulative propagative infection [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 292 . |
| MLA | Li, Zhiqiang et al. "Candidatus Liberibacter asiaticus exploits cytoskeletal system of psyllid vector for circulative propagative infection" . | MICROBIOLOGICAL RESEARCH 292 (2025) . |
| APA | Li, Zhiqiang , Yang, Xiao , Guo, Yuxin , Zhang, Xiaofeng , Li, You , Kuo, Yen-Wen et al. Candidatus Liberibacter asiaticus exploits cytoskeletal system of psyllid vector for circulative propagative infection . | MICROBIOLOGICAL RESEARCH , 2025 , 292 . |
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Xenophagy is an important antibacterial defense mechanism that many organisms use to engulf intracellular pathogens. However, the mechanisms of xenophagy triggered by insect-borne plant bacteria are not well understood. Candidatus Liberibacter asiaticus (CLas) causes Huanglongbing, which poses a serious threat to citrus production. CLas is a phloem-limited unculturable bacterium that is transmitted by the Asian citrus psyllid in a persistent and propagative manner in nature. Here, we found that CLas infection in the gut of psyllids triggered a mild and anti-bacterial xenophagy. Xenophagy limited excessive propagation of CLas to maintain psyllid survival, because overload of CLas was detrimental to psyllid life. Furthermore, the outer membrane beta-barrel protein (OMBB) of CLas is the key secreted protein that induces xenophagy in psyllids by interacting with ATG8 and ATG14. OMBB can independently induce autophagy in psyllid and non-host cells. Together, these results revealed that an insect-borne plant bacterium activates mild xenophagy to control its propagation, thereby achieving persistent infection in insect vectors.
Keyword ：
Candidatus Liberibacter asiaticus Candidatus Liberibacter asiaticus Huanglongbing Huanglongbing Persistent infection Persistent infection Psyllid Psyllid Xenophagy Xenophagy
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| GB/T 7714 | Yu, Zhongkai , Guo, Yuxin , Chen, Hongyan et al. A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection [J]. | MICROBIOLOGICAL RESEARCH , 2025 , 297 . |
| MLA | Yu, Zhongkai et al. "A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection" . | MICROBIOLOGICAL RESEARCH 297 (2025) . |
| APA | Yu, Zhongkai , Guo, Yuxin , Chen, Hongyan , Wan, Wenqiang , Hu, Mengting , Li, You et al. A phloem-limited unculturable bacterium induces mild xenophagy in insect vectors for persistent infection . | MICROBIOLOGICAL RESEARCH , 2025 , 297 . |
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Insect-borne pathogens often reduce the reproductive fitness of insect vectors. Rice gall dwarf virus (RGDV) is biparentally transmitted to the offspring of its leafhopper vector. However, maternal transmission of RGDV decreases female fecundity and disrupts egg development via an unknown mechanism. This study reveals that RGDV induces mitochondria-dependent apoptosis in leafhopper ovaries, promoting viral infection but impairing ovary development. This apoptosis is transmitted to eggs during maternal RGDV transmission, promoting viral infection while harming eggs. RGDV in the ovary activates insulin-like peptide-driven PI3K signaling but reverses the downstream AKT/FoxO signaling axis. This reversal activates FoxO, which in turn transcribes pro-apoptotic Bcl-2-related ovarian killer, triggering mitochondria-dependent apoptosis. Moreover, RGDV capsid protein P2 is the key viral protein responsible for inducing apoptosis through the PI3K/AKT/FoxO signaling axis. Specifically, P2 initiates mitochondria-dependent apoptosis by activating the PI3K signaling pathway upon recognition by insulin-like peptide 2. Furthermore, P2 reverses the AKT/FoxO signaling axis via its interaction with PTEN. In contrast, two rice viruses, which are exclusively maternally transmitted, do not induce apoptosis in the ovary of insect vectors. These findings uncover how this biparentally transmitted plant virus induces inheritable apoptosis, thereby imposing female reproductive costs, and highlight viral manipulation of vector reproduction to optimize transmission.
Keyword ：
apoptosis apoptosis maternal transmission maternal transmission PI3K/AKT/FoxO signaling PI3K/AKT/FoxO signaling plant virus plant virus reproductive costs reproductive costs
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| GB/T 7714 | Wu, Haibo , Wan, Wenqiang , Liang, Qingquan et al. Plant Virus-Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental Transmission [J]. | ADVANCED SCIENCE , 2025 . |
| MLA | Wu, Haibo et al. "Plant Virus-Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental Transmission" . | ADVANCED SCIENCE (2025) . |
| APA | Wu, Haibo , Wan, Wenqiang , Liang, Qingquan , Yang, Hengsong , Lu, Chengcong , Wei, Taiyun et al. Plant Virus-Induced Inheritable Apoptosis Drives Reproductive Costs in Female Insect Vectors to Balance Viral Biparental Transmission . | ADVANCED SCIENCE , 2025 . |
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Banana Fusarium wilt, which is known as Panama disease and caused by Fusarium oxysporum f. sp. cubense (Foc), is a destructive disease that can lead to plant death and complete loss of banana plantations. For obtaining resistant varieties against Fusarium wilt, research is required to elucidate the mechanisms of genetic resistance to Foc. In this research, the banana plants infected with banana bunchy top virus (BBTV) showed resistance to Foc. RNA-seq results showed that the infection with Foc upregulated many resistance-related genes in BBTV-positive plants, especially genes related to ROS production. It was also found that more H2O2 in BBTV-positive plants was induced during Foc infection. The movement protein (MP) of BBTV could increase H2O2 levels by promoting the accumulation of MaSGT1a (suppressor of the G-two allele of SKP1) via direct interaction. Thus, the resistance to Foc induced by BBTV may attribute to MP increasing MaSGT1a protein levels to induce ROS production. MaSGT1a could promote the accumulation of R proteins MaRPM1 and MaRPP8, and increase H2O2 levels in Nicotiana benthamiana. The expression of R proteins in Nicotiana benthamiana caused an increase in H2O2 levels and promoted resistance to Foc. Thus, the MP of BBTV could induce plant defense through interaction with MaSGT1a. We speculate that MaSGT1a is likely involved in R gene-mediated resistance and thereby promotes the resistance of banana against Foc.
Keyword ：
Banana Fusarium wilt Banana Fusarium wilt BBTV BBTV MP MP ROS ROS R protein R protein SGT1 SGT1
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| GB/T 7714 | Wang, Weiying , Wan, Wenqiang , Chen, Qian et al. Banana bunchy top virus movement protein induces resistance in banana against Fusarium wilt [J]. | PHYTOPATHOLOGY RESEARCH , 2024 , 6 (1) . |
| MLA | Wang, Weiying et al. "Banana bunchy top virus movement protein induces resistance in banana against Fusarium wilt" . | PHYTOPATHOLOGY RESEARCH 6 . 1 (2024) . |
| APA | Wang, Weiying , Wan, Wenqiang , Chen, Qian , Wei, Taiyun , Zhang, Hongxiang . Banana bunchy top virus movement protein induces resistance in banana against Fusarium wilt . | PHYTOPATHOLOGY RESEARCH , 2024 , 6 (1) . |
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BACKGROUND: Vitellogenin (Vg), known as the yolk protein precursor for oocyte development in female insects, can be secreted to plant host from salivary glands of hemipterans, including rice leafhopper Recilia dorsalis. The aim of this study was to investigate the function of salivary-secreted Vg of R. dorsalis (RdVg) in rice host. We propose that RdVg possibly regulates the rice defense against insects, benefiting R. dorsalis feeding. RESULTS: RdVg was released into rice phloem along with saliva during R. dorsalis feeding. Knocking down RdVg increased the level of H2O2 and improved H2O2 metabolism in rice plants, making it difficult for R. dorsalis to feed. The transient expression or overexpression of the lipoprotein N-terminal domain of RdVg (RdVg2) significantly reduced hydrogen peroxide (H2O2) metabolism in plants. This suggests that salivary-secreted RdVg acts as an effector suppressing the H2O2 burst in rice plants, and RdVg2 is the key domain. RdVg2 could interact with rice sulfite oxidase (OsSO), which catalyzes the oxidation of SO32- and produces H2O2. Exposure of rice plants to R. dorsalis, overexpression of RdVg2 or knocking out OsSO reduced OsSO accumulation and SO(3)(2- )oxidation, benefiting R. dorsalis feeding. However overexpression of OsSO increased SO32- oxidation and H2O2 metabolism, inhibiting R. dorsalis feeding. CONCLUSION: RdVg inhibits H2O2 generation via suppressing OsSO accumulation, ultimately benefiting R. dorsalis feeding. These findings identify RdVg as an effector that suppresses plant defense to insects, and provide insights into the function of salivary-secreted Vg in other Hemiptera insects . (c) 2024 Society of Chemical Industry.
Keyword ：
H2O2 burst H2O2 burst leafhopper leafhopper sulfite oxidase sulfite oxidase Vitellogenin Vitellogenin
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| GB/T 7714 | Lu, Chengcong , Zhang, Yating , Xu, Yuanyuan et al. Salivary-secreted vitellogenin suppresses H2O2 burst of plants facilitating Recilia dorsalis leafhopper feeding [J]. | PEST MANAGEMENT SCIENCE , 2024 , 80 (12) : 6222-6235 . |
| MLA | Lu, Chengcong et al. "Salivary-secreted vitellogenin suppresses H2O2 burst of plants facilitating Recilia dorsalis leafhopper feeding" . | PEST MANAGEMENT SCIENCE 80 . 12 (2024) : 6222-6235 . |
| APA | Lu, Chengcong , Zhang, Yating , Xu, Yuanyuan , Wei, Taiyun , Chen, Qian . Salivary-secreted vitellogenin suppresses H2O2 burst of plants facilitating Recilia dorsalis leafhopper feeding . | PEST MANAGEMENT SCIENCE , 2024 , 80 (12) , 6222-6235 . |
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