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学者姓名：张帅
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Abstract ：
Rice viruses frequently cause major yield losses, threatening global food security. In this study, we employed a multi-omics approach (transcriptome, small RNA and metabolome) to investigate the common and distinct responses of rice to three dwarfing viruses, rice ragged stunt virus, rice grassy stunt virus and Southern rice black-streaked dwarf virus. All three pathogens elicited notable dwarfing and increased tillering in infected plants, which significantly impacted yield potential. We identified thousands of DEGs and metabolites, revealing significant accumulation of phenylpropanoid intermediates, such as ferulic acid (FA) and cinnamic acid (CA). Despite the downregulation of lignin biosynthesis genes, total lignin content rose markedly, which indicated a feedback regulation mechanism that reinforced cell walls. Hormone signalling pathways were also reprogrammed, with elevated jasmonic acid (JA) and suppressed ethylene and abscisic acid pathways, which potentially weakened certain defences. Exogenous application of FA and CA effectively promoted lignin deposition and exerted a degree of inhibition on brown planthopper feeding. These findings highlighted the phenylpropanoid pathway as a critical nexus that linked plant structural reinforcement and defence responses. Our study provided novel targets for breeding virus-resistant rice cultivars and offered a broader framework for understanding plant-pathogen-insect interactions.
Keyword ：
brown planthopper feeding brown planthopper feeding lignin accumulation lignin accumulation multi-omics analysis multi-omics analysis phenylpropanoid pathway phenylpropanoid pathway rice viral infections rice viral infections
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| GB/T 7714 | Lue, Shaoyuan , Zhu, Zhihong , Yu, Xiyuan et al. Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) : 6170-6184 . |
| MLA | Lue, Shaoyuan et al. "Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV" . | PLANT CELL AND ENVIRONMENT 48 . 8 (2025) : 6170-6184 . |
| APA | Lue, Shaoyuan , Zhu, Zhihong , Yu, Xiyuan , Dong, Chaoyi , Cao, Jiamin , Zhang, Zhiyi et al. Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) , 6170-6184 . |
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Liquid-liquid phase separation (LLPS) regulates diverse biological functions by mediating the assembly of biomolecular condensates. However, it remains unclear how host LLPS is targeted by viruses during infection. Here we show that a plant bunyaviral protein, the disease-specific protein (SP) encoded by rice stripe virus (RSV), possesses phase separation potential through its N-terminal intrinsically disordered region 1 (IDR1). In vivo, however, SP does not form phase-separated biomolecular condensates independently but utilizes its phase separation properties to interfere with the phase separation of the SERRATE protein (SE), a key component of Dicing bodies essential for microRNA processing. By disrupting SE phase separation, SP inhibits D-body assembly and miRNA biogenesis. Our study demonstrates that a viral protein can modulate host microRNA processing by targeting LLPS, revealing a previously uncharacterized mechanism involved in viral infection strategies and miRNA biogenesis regulation in plants.
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| GB/T 7714 | Zou, Jing , Zhang, Shuai , Chen, Ying et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis [J]. | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| MLA | Zou, Jing et al. "A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis" . | NATURE COMMUNICATIONS 16 . 1 (2025) . |
| APA | Zou, Jing , Zhang, Shuai , Chen, Ying , He, Chun , Pan, Xin , Zhang, Yimin et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis . | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
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本发明属于生物技术领域，具体涉及OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用。所述OsMADS15的氨基酸序列如SEQ ID NO.2所示，其编码基因的核苷酸序列如SEQ ID NO.1所示。本发明首次发现，抑制水稻中OsMADS15蛋白的编码基因的表达，可以降低水稻对水稻草状矮化病毒的抗性。因此，OsMADS15蛋白及其编码基因对于研究水稻草状矮化病，提供敏感型株系，具有重要意义。
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| GB/T 7714 | 吴建国 , 赵珊珊 , 吴元圣 et al. OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用 : CN202510323689.X[P]. | 2025-03-19 . |
| MLA | 吴建国 et al. "OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用" : CN202510323689.X. | 2025-03-19 . |
| APA | 吴建国 , 赵珊珊 , 吴元圣 , 张帅 , 李川 . OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用 : CN202510323689.X. | 2025-03-19 . |
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Rice, a major global food staple, is threatened by viral infections that hinder its growth and yield. We have recently shown that the virulence protein P3 of rice grassy stunt virus promotes pathogenesis by inducing proteasome-controlled degradation of the rice RNA polymerase IV (RNA Pol IV) protein NRPD1a controlled by the P3-interacting E3 ubiquitin ligase P3IP1. However, the underlying mechanisms remain elusive. In this study, we show that P3 acts as a virus-encoded transcription activator-like effector to upregulate transcription of somatic embryogenesis receptor kinase 4 (SERK4) by directly binding to its promoter. SERK4 phosphorylates P3IP1 and enhances RNA Pol IVa (NRPD1a) degradation following P3IP1-controlled ubiquitination, leading to attenuated antiviral defense in rice. Thus, our study finds a critical viral virulence strategy by encoding a transcription factor-like protein that activates a host kinase to promote proteasome-controlled degradation of NRPD1a, thereby disarming RNA-directed DNA methylation (RdDM) antiviral defense.
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| GB/T 7714 | Wu, Yuansheng , Zhao, Shanshan , Liu, Na et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity [J]. | DEVELOPMENTAL CELL , 2025 , 60 (17) : 2348-2362 . |
| MLA | Wu, Yuansheng et al. "A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity" . | DEVELOPMENTAL CELL 60 . 17 (2025) : 2348-2362 . |
| APA | Wu, Yuansheng , Zhao, Shanshan , Liu, Na , Wu, Fengling , Huang, Shiting , Li, Dongyuan et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity . | DEVELOPMENTAL CELL , 2025 , 60 (17) , 2348-2362 . |
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本发明公开了一种多基因聚合创建兼抗褐飞虱和南方黑条矮缩病水稻材料的方法，属于水稻分子育种技术领域。本发明选用生产上推广面积较大且综合性状优良的恢复系9311做轮回亲本，分别构建了7个抗性基因的近等基因系材料。再利用不同基因的近等基因系两两杂交，结合分子标记辅助选择和常规育种技术，共获得了81个分别携带双基因、三基因、四基因、五基因和六基因纯合的材料。通过田间发病率调查和人工接虫鉴定，最终筛选出9份兼抗褐飞虱和南方黑条矮缩病的水稻材料。利用本发明方法选育的品系在产量和抗性得到较好的协调，在病虫害抗性上得到了显著提高。
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| GB/T 7714 | 吴建国 , 胡杰 , 黄显波 et al. 一种多基因聚合创建兼抗褐飞虱和南方黑条矮缩病水稻材料的方法 : CN202510382223.7[P]. | 2025-03-28 . |
| MLA | 吴建国 et al. "一种多基因聚合创建兼抗褐飞虱和南方黑条矮缩病水稻材料的方法" : CN202510382223.7. | 2025-03-28 . |
| APA | 吴建国 , 胡杰 , 黄显波 , 张帅 , 赵珊珊 . 一种多基因聚合创建兼抗褐飞虱和南方黑条矮缩病水稻材料的方法 : CN202510382223.7. | 2025-03-28 . |
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本发明公开一种多基因聚合创建持久广谱抗褐飞虱水稻材料的方法，属于水稻分子育种技术领域。本发明选用生产上推广面积较大且综合性状优良的籼型水稻9311做轮回亲本，构建7个抗性基因的近等基因系材料，再利用不同基因的近等基因系两两杂交和完全双列杂交，结合分子标记辅助选择、基因芯片、常规育种技术和褐飞虱抗性鉴定，共获得了4种双基因聚合水稻材料，即BPH6/BPH33,BPH14/BPH33,BPH31/BPH33和BPH32/BPH33为具有持久广谱抗褐飞虱的新型水稻材料。利用本发明方法选育的品系在产量和抗性得到较好的协调，在抗褐飞虱持久广谱抗性上得到了显著提高。
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| GB/T 7714 | 胡杰 , 吴建国 , 张帅 et al. 一种多基因聚合创建持久广谱抗褐飞虱水稻材料的方法 : CN202510380520.8[P]. | 2025-03-28 . |
| MLA | 胡杰 et al. "一种多基因聚合创建持久广谱抗褐飞虱水稻材料的方法" : CN202510380520.8. | 2025-03-28 . |
| APA | 胡杰 , 吴建国 , 张帅 , 赵珊珊 . 一种多基因聚合创建持久广谱抗褐飞虱水稻材料的方法 : CN202510380520.8. | 2025-03-28 . |
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本发明公开了一种聚合7个抗褐飞虱基因的水稻材料的创建方法。选用9311做轮回亲本构建近等基因系材料，两两杂交，结合分子标记辅助选择和常规育种技术实现7个抗性基因的聚合；通过人工接虫鉴定和全生育期的基本农艺性状评价，获得褐飞虱抗性明显增强的改良品系。利用本发明筛选出聚合3个及以上基因聚合的个体，效率高达98.5%。采用7个抗性基因杂合型的材料进行基因聚合育种，能够实现后代个体的多基因聚合，从而保证褐飞虱抗性达到一定水平。分离后代中，大部分的基因聚合个体彼此之间携带的基因组合不同，有效保证了基因多样性，避免了单一基因容易失效的问题，为实现对褐飞虱的持久抗性提供了可能。
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| GB/T 7714 | 吴建国 , 胡杰 , 张帅 et al. 一种聚合7个抗褐飞虱基因的水稻材料的创建方法 : CN202510383632.9[P]. | 2025-03-28 . |
| MLA | 吴建国 et al. "一种聚合7个抗褐飞虱基因的水稻材料的创建方法" : CN202510383632.9. | 2025-03-28 . |
| APA | 吴建国 , 胡杰 , 张帅 , 赵珊珊 . 一种聚合7个抗褐飞虱基因的水稻材料的创建方法 : CN202510383632.9. | 2025-03-28 . |
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本发明公开了miR1868在调控水稻分蘖和抗病毒中的应用，本方案提供了基因Osa‑miR1868(简称miR1868)和Pre‑miR1868(miR1868前体)在调控植物分蘖和对病毒病抗性中的应用；本方案将编码Pre‑miR1868的DNA分子导入目的的植物，得到转基因植物，所述转基因植物的分蘖少于受体植物，但是抗病性高于受体植物。说明Pre‑miR1868超量表达减少了植物分蘖，但是提高了植物对病毒病的耐受性。Pre‑miR1868具有调控植物分蘖和病毒抗性的功能，对于培育抗病毒植物，提高植物产量具有重大价值。
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| GB/T 7714 | 吴建国 , 张宝刚 , 张帅 et al. miR1868在调控水稻分蘖和抗病毒中的应用 : CN202411014313.2[P]. | 2024-07-26 . |
| MLA | 吴建国 et al. "miR1868在调控水稻分蘖和抗病毒中的应用" : CN202411014313.2. | 2024-07-26 . |
| APA | 吴建国 , 张宝刚 , 张帅 , 赵珊珊 , 张雄 . miR1868在调控水稻分蘖和抗病毒中的应用 : CN202411014313.2. | 2024-07-26 . |
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本发明涉及生物技术领域，尤其涉及OsAGO12蛋白及其编码基因表达在调控植物对水稻瘤矮病毒抗性中的应用。OsAGO12蛋白的氨基酸序列如SEQ ID NO.2所示，其编码基因OsAGO12的核苷酸序列如SEQ ID NO.1所示。本发明采用过表达和CRISPR/Cas9技术分别将OsAGO12基因在水稻中进行过表达或者进行敲除突变，结果发现，在水稻中过表达OsAGO12基因能够显著增强水稻对水稻瘤矮病毒的抗性。本发明为水稻改良育种提供了新的基因资源。
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| GB/T 7714 | 吴建国 , 赵珊珊 , 吴明 et al. OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用 : CN202411115055.7[P]. | 2024-08-14 . |
| MLA | 吴建国 et al. "OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用" : CN202411115055.7. | 2024-08-14 . |
| APA | 吴建国 , 赵珊珊 , 吴明 , 张帅 . OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用 : CN202411115055.7. | 2024-08-14 . |
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本发明提供了一种基于多重RT‑PCR的蝴蝶兰病毒检测方法及其应用；本发明设计了针对齿舌兰轮斑病毒(ORSV)、烟草花叶病毒(TMV)、番茄斑驳嵌纹病毒(ToMMV)、辣椒轻斑驳病毒(PMMoV)、李痘病毒(PPV)和烟草蚀纹病毒(TEV)衣壳蛋白基因的特异性引物，并采用多重RT‑PCR实现对多个病毒的高效、灵敏和特异性检测。本发明为蝴蝶兰生产和管理中病毒病害的早期诊断和控制提供了重要技术手段，能够有效减少病毒的传播风险，提升检疫措施的效果，适宜进一步推广应用。
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| GB/T 7714 | 吴建国 , 邹婧 , 张帅 et al. 基于多重RT-PCR的蝴蝶兰病毒检测方法及其应用 : CN202510132913.7[P]. | 2025-02-06 . |
| MLA | 吴建国 et al. "基于多重RT-PCR的蝴蝶兰病毒检测方法及其应用" : CN202510132913.7. | 2025-02-06 . |
| APA | 吴建国 , 邹婧 , 张帅 , 胡群 , 陈莹 . 基于多重RT-PCR的蝴蝶兰病毒检测方法及其应用 : CN202510132913.7. | 2025-02-06 . |
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