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学者姓名：张帅
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Abstract ：
Liquid-liquid phase separation (LLPS) regulates diverse biological functions by mediating the assembly of biomolecular condensates. However, it remains unclear how host LLPS is targeted by viruses during infection. Here we show that a plant bunyaviral protein, the disease-specific protein (SP) encoded by rice stripe virus (RSV), possesses phase separation potential through its N-terminal intrinsically disordered region 1 (IDR1). In vivo, however, SP does not form phase-separated biomolecular condensates independently but utilizes its phase separation properties to interfere with the phase separation of the SERRATE protein (SE), a key component of Dicing bodies essential for microRNA processing. By disrupting SE phase separation, SP inhibits D-body assembly and miRNA biogenesis. Our study demonstrates that a viral protein can modulate host microRNA processing by targeting LLPS, revealing a previously uncharacterized mechanism involved in viral infection strategies and miRNA biogenesis regulation in plants.
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| GB/T 7714 | Zou, Jing , Zhang, Shuai , Chen, Ying et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis [J]. | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
| MLA | Zou, Jing et al. "A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis" . | NATURE COMMUNICATIONS 16 . 1 (2025) . |
| APA | Zou, Jing , Zhang, Shuai , Chen, Ying , He, Chun , Pan, Xin , Zhang, Yimin et al. A plant bunyaviral protein disrupts SERRATE phase separation to modulate microRNA biogenesis during viral pathogenesis . | NATURE COMMUNICATIONS , 2025 , 16 (1) . |
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本发明提供了一种基于多重RT‑PCR的蝴蝶兰病毒检测方法及其应用；本发明设计了针对齿舌兰轮斑病毒(ORSV)、烟草花叶病毒(TMV)、番茄斑驳嵌纹病毒(ToMMV)、辣椒轻斑驳病毒(PMMoV)、李痘病毒(PPV)和烟草蚀纹病毒(TEV)衣壳蛋白基因的特异性引物，并采用多重RT‑PCR实现对多个病毒的高效、灵敏和特异性检测。本发明为蝴蝶兰生产和管理中病毒病害的早期诊断和控制提供了重要技术手段，能够有效减少病毒的传播风险，提升检疫措施的效果，适宜进一步推广应用。
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| GB/T 7714 | 吴建国 , 邹婧 , 张帅 et al. 基于多重RT-PCR的蝴蝶兰病毒检测方法及其应用 : CN202510132913.7[P]. | 2025-02-06 . |
| MLA | 吴建国 et al. "基于多重RT-PCR的蝴蝶兰病毒检测方法及其应用" : CN202510132913.7. | 2025-02-06 . |
| APA | 吴建国 , 邹婧 , 张帅 , 胡群 , 陈莹 . 基于多重RT-PCR的蝴蝶兰病毒检测方法及其应用 : CN202510132913.7. | 2025-02-06 . |
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本发明公开了miR1850在调控水稻抗水稻锯齿叶矮缩病毒中的应用，本方案披露了RRSV对miR1850的诱导，揭示了miR1850在水稻与RRSV互作中的功能，通过操纵miR1850的积累量将改变水稻对病毒的抗性。基于此，可以将低丰度的miR1850作为水稻抗病毒品种的一个筛选指标，这一发现为筛选miR1850下游的抗病毒靶标基因提供了依据，有助于缩小抗病毒筛选范围，加快基于MIR1850介导的多靶标抗病毒品种的培育。
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| GB/T 7714 | 吴建国 , 张宝刚 , 赵珊珊 et al. miR1850在调控水稻抗水稻锯齿叶矮缩病毒中的应用 : CN202411694639.4[P]. | 2024-11-25 . |
| MLA | 吴建国 et al. "miR1850在调控水稻抗水稻锯齿叶矮缩病毒中的应用" : CN202411694639.4. | 2024-11-25 . |
| APA | 吴建国 , 张宝刚 , 赵珊珊 , 张帅 . miR1850在调控水稻抗水稻锯齿叶矮缩病毒中的应用 : CN202411694639.4. | 2024-11-25 . |
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The present disclosure locates a new brown planthopper resistance gene BPH33.2 on the short arm of rice chromosome 4 by extreme mixed pool analysis (BSA-seq), and genetically transforming the gene BPH33.2 to make the susceptible rice to be the brown planthopper resistant phenotype rice; at the same time, the knockout of the gene BPH33.2 was used to cause the loss of the brown planthopper resistant phenotype in insect resistant rice, thus confirming the function of BPH33.2. The present disclosure also provides molecular markers closely linked with the rice brown planthopper resistance gene BPH33.2. Through experimental detection of molecular markers linked or co-segregated with these resistance loci, the brown planthopper resistance of rice plants can be accurately predicted at the seedling stage, accelerating the progress of the selection of brown planthopper-resistant rice varieties.
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| GB/T 7714 | JIANGUO WU , JIE HU , SHUAI ZHANG et al. METHOD FOR PREPARING BROWN PLANTHOPPER RESISTANT PLANTS USING RICE BROWN PLANTHOPPER RESISTANCE GENE BPH33.2 : US202418414498A[P]. | 2024-01-17 . |
| MLA | JIANGUO WU et al. "METHOD FOR PREPARING BROWN PLANTHOPPER RESISTANT PLANTS USING RICE BROWN PLANTHOPPER RESISTANCE GENE BPH33.2" : US202418414498A. | 2024-01-17 . |
| APA | JIANGUO WU , JIE HU , SHUAI ZHANG , SHANSHAN ZHAO , XIAOQING WU . METHOD FOR PREPARING BROWN PLANTHOPPER RESISTANT PLANTS USING RICE BROWN PLANTHOPPER RESISTANCE GENE BPH33.2 : US202418414498A. | 2024-01-17 . |
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Rice, a major global food staple, is threatened by viral infections that hinder its growth and yield. We have recently shown that the virulence protein P3 of rice grassy stunt virus promotes pathogenesis by inducing proteasome-controlled degradation of the rice RNA polymerase IV (RNA Pol IV) protein NRPD1a controlled by the P3-interacting E3 ubiquitin ligase P3IP1. However, the underlying mechanisms remain elusive. In this study, we show that P3 acts as a virus-encoded transcription activator-like effector to upregulate transcription of somatic embryogenesis receptor kinase 4 (SERK4) by directly binding to its promoter. SERK4 phosphorylates P3IP1 and enhances RNA Pol IVa (NRPD1a) degradation following P3IP1-controlled ubiquitination, leading to attenuated antiviral defense in rice. Thus, our study finds a critical viral virulence strategy by encoding a transcription factor-like protein that activates a host kinase to promote proteasome-controlled degradation of NRPD1a, thereby disarming RNA-directed DNA methylation (RdDM) antiviral defense.
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| GB/T 7714 | Wu, Yuansheng , Zhao, Shanshan , Liu, Na et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity [J]. | DEVELOPMENTAL CELL , 2025 , 60 (17) : 2348-2362 . |
| MLA | Wu, Yuansheng et al. "A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity" . | DEVELOPMENTAL CELL 60 . 17 (2025) : 2348-2362 . |
| APA | Wu, Yuansheng , Zhao, Shanshan , Liu, Na , Wu, Fengling , Huang, Shiting , Li, Dongyuan et al. A virulence protein activates SERK4 and degrades RNA polymerase IV protein to suppress rice antiviral immunity . | DEVELOPMENTAL CELL , 2025 , 60 (17) , 2348-2362 . |
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本发明属于生物技术领域，具体涉及OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用。所述OsMADS15的氨基酸序列如SEQ ID NO.2所示，其编码基因的核苷酸序列如SEQ ID NO.1所示。本发明首次发现，抑制水稻中OsMADS15蛋白的编码基因的表达，可以降低水稻对水稻草状矮化病毒的抗性。因此，OsMADS15蛋白及其编码基因对于研究水稻草状矮化病，提供敏感型株系，具有重要意义。
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| GB/T 7714 | 吴建国 , 赵珊珊 , 吴元圣 et al. OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用 : CN202510323689.X[P]. | 2025-03-19 . |
| MLA | 吴建国 et al. "OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用" : CN202510323689.X. | 2025-03-19 . |
| APA | 吴建国 , 赵珊珊 , 吴元圣 , 张帅 , 李川 . OsMADS15蛋白及其编码基因在调控植物对水稻草状矮化病毒抗性中的应用 : CN202510323689.X. | 2025-03-19 . |
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本发明涉及生物技术领域，尤其涉及OsAGO12蛋白及其编码基因表达在调控植物对水稻瘤矮病毒抗性中的应用。OsAGO12蛋白的氨基酸序列如SEQ ID NO.2所示，其编码基因OsAGO12的核苷酸序列如SEQ ID NO.1所示。本发明采用过表达和CRISPR/Cas9技术分别将OsAGO12基因在水稻中进行过表达或者进行敲除突变，结果发现，在水稻中过表达OsAGO12基因能够显著增强水稻对水稻瘤矮病毒的抗性。本发明为水稻改良育种提供了新的基因资源。
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| GB/T 7714 | 吴建国 , 赵珊珊 , 吴明 et al. OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用 : CN202411115055.7[P]. | 2024-08-14 . |
| MLA | 吴建国 et al. "OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用" : CN202411115055.7. | 2024-08-14 . |
| APA | 吴建国 , 赵珊珊 , 吴明 , 张帅 . OsAGO12蛋白及其编码基因在调控植物对水稻瘤矮病毒抗性中的应用 : CN202411115055.7. | 2024-08-14 . |
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本发明涉及水稻绿色革命基因SD1在调控水稻病毒抗性中的应用。本发明通过CRISPR‑Cas9技术敲除水稻kasalath中绿色革命基因SD1，获得SD1敲除转基因材料。病毒接种试验显示，SD1敲除转基因材料在感染RRSV、SRBSDV、RGSV和RSV后，其病症较野生型更加明显，各病毒侵染率显著高于野生型，从而揭示了水稻绿色革命基因SD1基因在调控水稻病毒抗性中的作用。本发明为水稻病毒抗性机制的研究和抗病育种提供了新的分子靶点和基因资源。
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| GB/T 7714 | 张帅 , 吴建国 , 赵珊珊 et al. 水稻绿色革命基因SD1在调控水稻病毒抗性中的应用 : CN202510658169.4[P]. | 2025-05-21 . |
| MLA | 张帅 et al. "水稻绿色革命基因SD1在调控水稻病毒抗性中的应用" : CN202510658169.4. | 2025-05-21 . |
| APA | 张帅 , 吴建国 , 赵珊珊 , 胡琼 . 水稻绿色革命基因SD1在调控水稻病毒抗性中的应用 : CN202510658169.4. | 2025-05-21 . |
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本发明公开了OsPUB16基因在水稻抗褐飞虱中的应用。所述OsPUB16基因的核苷酸序列如SEQ ID NO.1所示，其编码的蛋白的氨基酸序列如SEQ ID NO.2所示。本发明对OsPUB16基因进行过量表达和敲除，抗性鉴定后发现过量表达OsPUB16基因会降低水稻对褐飞虱的抗性，而敲除OsPUB16基因会提高水稻对褐飞虱的抗性。本发明这一发现为选育出更具褐飞虱抗性的水稻品种提供了新的思路和方向。
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| GB/T 7714 | 吴建国 , 赵珊珊 , 张帅 et al. OsPUB16基因在水稻抗褐飞虱中的应用 : CN202510713764.3[P]. | 2025-05-30 . |
| MLA | 吴建国 et al. "OsPUB16基因在水稻抗褐飞虱中的应用" : CN202510713764.3. | 2025-05-30 . |
| APA | 吴建国 , 赵珊珊 , 张帅 , 吴元圣 , 黄诗婷 , 朱志鸿 . OsPUB16基因在水稻抗褐飞虱中的应用 : CN202510713764.3. | 2025-05-30 . |
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Rice viruses frequently cause major yield losses, threatening global food security. In this study, we employed a multi-omics approach (transcriptome, small RNA and metabolome) to investigate the common and distinct responses of rice to three dwarfing viruses, rice ragged stunt virus, rice grassy stunt virus and Southern rice black-streaked dwarf virus. All three pathogens elicited notable dwarfing and increased tillering in infected plants, which significantly impacted yield potential. We identified thousands of DEGs and metabolites, revealing significant accumulation of phenylpropanoid intermediates, such as ferulic acid (FA) and cinnamic acid (CA). Despite the downregulation of lignin biosynthesis genes, total lignin content rose markedly, which indicated a feedback regulation mechanism that reinforced cell walls. Hormone signalling pathways were also reprogrammed, with elevated jasmonic acid (JA) and suppressed ethylene and abscisic acid pathways, which potentially weakened certain defences. Exogenous application of FA and CA effectively promoted lignin deposition and exerted a degree of inhibition on brown planthopper feeding. These findings highlighted the phenylpropanoid pathway as a critical nexus that linked plant structural reinforcement and defence responses. Our study provided novel targets for breeding virus-resistant rice cultivars and offered a broader framework for understanding plant-pathogen-insect interactions.
Keyword ：
brown planthopper feeding brown planthopper feeding lignin accumulation lignin accumulation multi-omics analysis multi-omics analysis phenylpropanoid pathway phenylpropanoid pathway rice viral infections rice viral infections
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| GB/T 7714 | Lue, Shaoyuan , Zhu, Zhihong , Yu, Xiyuan et al. Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV [J]. | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) : 6170-6184 . |
| MLA | Lue, Shaoyuan et al. "Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV" . | PLANT CELL AND ENVIRONMENT 48 . 8 (2025) : 6170-6184 . |
| APA | Lue, Shaoyuan , Zhu, Zhihong , Yu, Xiyuan , Dong, Chaoyi , Cao, Jiamin , Zhang, Zhiyi et al. Phenylpropanoid Metabolites Mediate Antiviral Defense and Vector Resistance in Rice Infected With RRSV, RGSV, and SRBSDV . | PLANT CELL AND ENVIRONMENT , 2025 , 48 (8) , 6170-6184 . |
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