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学者姓名：曾黎辉
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Abstract ：
Sucrose is an important factor affecting plant growth and fruit quality, but the molecular regulatory mechanism of sucrose biosynthesis in longan is not yet understood. Here, we characterized a transcription factor, DlbHLH68, positively regulates sucrose accumulation in longan. Subcellular localization and transcriptional activity analysis indicated that DlbHLH68 is a nuclear transcriptional activator. Overexpressing DlbHLH68 in Arabidopsis enhanced sucrose content, plant height, and the relative expression level of sucrose phosphate synthase genes ( AtSPS1 and AtSPS2). Yeast one-hybrid and dual-luciferase reporter assays indicated that DlbHLH68 was able to activate the expression of DlSPS1, the homology gene of AtSPS1. As expected, overexpression of DlSPS1 significantly increased the sucrose content in transgenic Arabidopsis and longan fruits. Collectively, this study reveals that DlbHLH68 is a positive regulator in sucrose accumulation by activating DlSPS1 expression to mediate sucrose biosynthesis, which is helpful for understanding the molecular basis of sucrose biosynthesis and accumulation in longan fruit and provides candidate genes for further breeding.
Keyword ：
bHLH bHLH Fruit quality Fruit quality Longan Longan SPS SPS Sucrose Sucrose Transcriptional regulation Transcriptional regulation
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| GB/T 7714 | Fang, Ting , Li, Yun , Xie, Tao et al. The bHLH transcription factor DlbHLH68 positively regulates DlSPS1 expression to promote sucrose biosynthesis in longan [J]. | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 296 . |
| MLA | Fang, Ting et al. "The bHLH transcription factor DlbHLH68 positively regulates DlSPS1 expression to promote sucrose biosynthesis in longan" . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 296 (2025) . |
| APA | Fang, Ting , Li, Yun , Xie, Tao , Xian, Huimin , Bao, Yuying , Zeng, Lihui . The bHLH transcription factor DlbHLH68 positively regulates DlSPS1 expression to promote sucrose biosynthesis in longan . | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES , 2025 , 296 . |
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Passion fruit (Passiflora edulis Sims), belonging to the Passifloraceae family, is an economically important plant in tropical and subtropical regions. The advances in functional genomics research of passion fruit have been significantly hindered by its recalcitrance to regeneration and stable transformation. This study establishes the first efficient Agrobacterium rhizogenes-mediated hairy root transformation system for passion fruit. Utilizing the eGFP marker gene, transformation efficiencies of 11.3% were initially achieved with strains K599, MSU440, and C58C1, with K599 proving most effective. Key transformation parameters were systematically optimized to achieve the following: OD600 = 0.6, infection duration 30 min, acetosyringone concentration 100 mu M, and a dark co-cultivation period of 2 days. The system's utility was further enhanced by incorporating the red visual marker RUBY, enabling direct, instrument-free identification of transgenic roots via betaxanthin accumulation. Finally, this system was applied for functional analysis using PeMYB123, which may be involved in proanthocyanidin accumulation. Overexpression of PeMYB123 produced a higher content of proanthocyanidin in hairy roots. Additionally, the PeANR gene involved in the proanthocyanidin pathway was strongly activated in the transgenic hairy roots. This rapid and efficient visually simplified hairy root transformation system provides a powerful tool for functional gene studies in passion fruit.
Keyword ：
Agrobacterium rhizogenes Agrobacterium rhizogenes hairy root hairy root passion fruit passion fruit PeMYB123 PeMYB123 proanthocyanidin proanthocyanidin
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| GB/T 7714 | Pan, Jiayi , Zheng, Yiping , Wang, Tiancai et al. Establishment of an Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation System for Functional Analysis in Passion Fruit [J]. | PLANTS-BASEL , 2025 , 14 (15) . |
| MLA | Pan, Jiayi et al. "Establishment of an Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation System for Functional Analysis in Passion Fruit" . | PLANTS-BASEL 14 . 15 (2025) . |
| APA | Pan, Jiayi , Zheng, Yiping , Wang, Tiancai , Xiong, Pengpeng , Cui, Kaibo , Zeng, Lihui et al. Establishment of an Efficient Agrobacterium rhizogenes-Mediated Hairy Root Transformation System for Functional Analysis in Passion Fruit . | PLANTS-BASEL , 2025 , 14 (15) . |
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Passion fruit is one of the most famous fruit crops in tropical and subtropical regions due to its high edible, medicinal, and ornamental value. Flavonoids, a class of plant secondary metabolites, have important health-related roles. In this study, a total of 151 flavonoid metabolites were identified, of which 25 key metabolites may be the main contributors to the purple phenotype. Using RNA sequencing, 11,180 differentially expressed genes (DEGs) were identified. Among these, 48 flavonoid biosynthesis genes (PAL, 4CL, C4H, CHS, CHI, F3H, DFR, ANS, and UFGT) and 123 transcription factors were identified. Furthermore, 12 distinct modules were identified through weighted gene coexpression network analysis, of which the brown module displays a robust positive correlation with numerous flavonoid metabolites. Overexpression of PeMYB114 significantly promoted flavonoids accumulation in tobacco leaves. Our study provided a key candidate gene for molecular breeding to improve color traits in passion fruit.
Keyword ：
flavonoids flavonoids metabolome metabolome passion fruit passion fruit PeMYB114 PeMYB114 transcriptome transcriptome
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| GB/T 7714 | Fang, Ting , Zheng, Yiping , Ma, Qicheng et al. Integrated Transcriptomic and Metabolomic Analysis Revealed Regulatory Mechanisms on Flavonoids Biosynthesis in the Skin of Passion Fruit (Passiflora spp.) [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (1) : 967-978 . |
| MLA | Fang, Ting et al. "Integrated Transcriptomic and Metabolomic Analysis Revealed Regulatory Mechanisms on Flavonoids Biosynthesis in the Skin of Passion Fruit (Passiflora spp.)" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 73 . 1 (2025) : 967-978 . |
| APA | Fang, Ting , Zheng, Yiping , Ma, Qicheng , Ren, Rui , Xian, Huimin , Zeng, Lihui . Integrated Transcriptomic and Metabolomic Analysis Revealed Regulatory Mechanisms on Flavonoids Biosynthesis in the Skin of Passion Fruit (Passiflora spp.) . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2025 , 73 (1) , 967-978 . |
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Longan (Dimocarpus longan L.), an economically important tropical/subtropical fruit species, faces production constraints due to unreliable floral induction processes. CONSTANS-like (COL) genes play an important role in the photoperiodic flowering pathway. In this study, a total of 10, 15 and 15 DlCOL genes were identified in longan cultivars HHZ, SX and JDB, respectively. Phylogenetic analysis showed that DlCOL genes were divided into three subgroups, and the members of each subgroup had conserved B-box and CCT domains, indicating potential binding interactions between DlCOL genes and flowering-related genes. Analysis of tissue-specific expression showed that all DlCOL genes were widely expressed in various organs of longan, and were preferentially expressed in the leaves. Notably, DlCOL9 exhibited preferential expression in apical buds during the physiological differentiation stage of floral bud induction. Transgenic Arabidopsis plants overexpressing DlCOL9 displayed a significantly shortened flowering time accompanied by the increasing expression of flowering genes AtFT, AtSOC1 and AtCO, indicating that DlCOL9 has the function of promoting flowering. Furthermore, DlCOL9 was co-expressed with key flowering regulators in longan, DlFKF1, DlGI and DlSOC1. Yeast one-hybrid combined with dual-luciferase assays demonstrated that both DlGI and DlFKF1 could bind to the promoter of DlCOL9 to enhance its expression, whereas DlCOL9 transcriptionally regulated DlSOC1 directly. Our results reveal a conserved DlGI/DlFKF1-DlCOL9-DlSOC1 regulatory module governing floral induction in longan which advance the functional understanding of the DlCOL gene family and provide a molecular basis for optimizing flowering regulation in longan production.
Keyword ：
CONSTANS-LIKE gene CONSTANS-LIKE gene Dimocarpus longan Dimocarpus longan Floral induction Floral induction Photoperiod Photoperiod
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| GB/T 7714 | He, Dayi , Wei, Chunyan , Liang, Fan et al. Genome-wide identification of CONSTANS-like genes in Dimocarpus longan and functional characterization of DlCOL9 revealing its role in floral induction [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 225 . |
| MLA | He, Dayi et al. "Genome-wide identification of CONSTANS-like genes in Dimocarpus longan and functional characterization of DlCOL9 revealing its role in floral induction" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 225 (2025) . |
| APA | He, Dayi , Wei, Chunyan , Liang, Fan , Huang, Yuanyuan , Fang, Ting , Deng, Chaojun et al. Genome-wide identification of CONSTANS-like genes in Dimocarpus longan and functional characterization of DlCOL9 revealing its role in floral induction . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 225 . |
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The sugars will eventually be exported transporters (SWEET) family is a class of sugar transporters that play crucial roles in plant growth, reproduction, and stresses response. The characteristics of 21 PeSWEETs, identified in passion fruit (Passiflora edulis Sims) and divided into four clades, were evaluated. Structural feature analysis showed that exon numbers ranged from 4 to 12, while motif 1, 2, and 3 were highly conserved. Analysis of RNAseq from different tissues revealed that PeSWEETs showed tissue-specificity. Additionally, Cis-acting regulatory element analysis indicated that abundant hormone-response and stress response elements were enriched in promoters of PeSWEETs. Exogenous abscisic acid (ABA) treatment led to an increase in the soluble sugar accumulation and up-regulated the expression level of PeSWEET3. The PeSWEET3 protein localizes on the plasma membrane and exhibits transport activity of fructose, glucose, and mannose. Furthermore, ectopic expression of PeSWEET3 significantly enhanced soluble sugar accumulation in leaves of transgenic tobacco. Collectively, these results lay a solid foundation for further exploration on the role of PeSWEETs.
Keyword ：
Abscisic acid Abscisic acid Passion fruit Passion fruit Soluble sugar Soluble sugar SWEET SWEET
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| GB/T 7714 | Ren, Rui , Chen, Yuxuan , Yu, Xiao et al. Identification and characterization of SWEET gene family in passion fruit reveals the involvement of PeSWEET3 in soluble sugar accumulation [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 224 . |
| MLA | Ren, Rui et al. "Identification and characterization of SWEET gene family in passion fruit reveals the involvement of PeSWEET3 in soluble sugar accumulation" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 224 (2025) . |
| APA | Ren, Rui , Chen, Yuxuan , Yu, Xiao , Peng, Xianrui , Zeng, Lihui , Fang, Ting . Identification and characterization of SWEET gene family in passion fruit reveals the involvement of PeSWEET3 in soluble sugar accumulation . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 224 . |
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Flower morphology is a critical ornamental trait in flowering plants. Elucidating the molecular mechanisms underlying flower development is essential for the breeding of diverse ornamental plant cultivars. The ABCE model genes are pivotal in regulating floral development in angiosperms. In order to understand the molecular mechanism of flower development in Clematis, nine ABCE model genes were identified from the transcriptome data of Clematis cv. 'Amethyst Beauty'. Quantitative real-time polymerase chain reaction (qRT-PCR) analysis revealed tissue-specific expression patterns among these genes. ClPI, ClSEP1, and ClSEP3 were predominantly expressed in sepals. ClAP3, ClPI, ClAG2, ClSEP1, and ClSEP3 were highly expressed in stamens. ClAG1 was exclusively expressed in pistils. These findings suggested that ABCE genes may play an important role in regulating the formation of flower morphology in the Clematis. The open reading frame (ORF) of ClAG2 was cloned and overexpression of ClAG2 in tobacco resulted in shorter corolla tube, reduced crown area, and stunted stamen. ClAG2 may have a negative effect on the formation of double-tepal flowers of Clematis and play a specific role in stamen and pistil development. Yeast two-hybrid assays demonstrated that ClAG2 could interact with class E proteins ClSEP3 and ClSEP4 but not with class B proteins ClAP3 and ClPI. Our results will lay a theoretical foundation for further research on the mechanism of flower development regulation in Clematis.
Keyword ：
ABCE model genes ABCE model genes AGAMOUS AGAMOUS Clematis Clematis expression analysis expression analysis floral morphology floral morphology
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| GB/T 7714 | Chen, Xuerong , Zhou, Ping , Guo, Nanhong et al. The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in Clematis L. [J]. | HORTICULTURAE , 2025 , 11 (1) . |
| MLA | Chen, Xuerong et al. "The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in Clematis L." . | HORTICULTURAE 11 . 1 (2025) . |
| APA | Chen, Xuerong , Zhou, Ping , Guo, Nanhong , Zheng, Yiping , Hou, Xiumei , Zeng, Lihui . The MADS-Box Transcription Factor ClAG2 Is a Key Regulator for the Formation of Double Flower in Clematis L. . | HORTICULTURAE , 2025 , 11 (1) . |
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Passion fruit (Passiflora spp.) is an important fruit tree in the family Passifloraceae. The color of the fruit skin, a significant agricultural trait, is determined by the content of anthocyanin in passion fruit. However, the regulatory mechanisms behind the accumulation of anthocyanin in different passion fruit skin colors remain unclear. In the study, we identified and characterized a R2R3-MYB transcription factor, PeMYB114, which functions as a transcriptional activator in anthocyanin biosynthesis. Yeast one-hybrid system and dual-luciferase analysis showed that PeMYB114 could directly activate the expression of anthocyanin structural genes (PeCHS and PeDFR). Furthermore, a natural variation in the promoter region of PeMYB114 alters its expression. PeMYB114(purple) accessions with the 224-bp insertion have a higher anthocyanin level than PeMYB114(yellow) accessions with the 224-bp deletion. The findings enhance our understanding of anthocyanin accumulation in fruits and provide genetic resources for genome design for improving passion fruit quality.
Keyword ：
anthocyanin anthocyanin indel indel molecular marker molecular marker passion fruit passion fruit PeMYB114 PeMYB114
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| GB/T 7714 | Fang, Ting , Wang, Mengzhen , He, Ruijie et al. A 224-bp Indel in the Promoter of PeMYB114 Accounts for Anthocyanin Accumulation of Skin in Passion Fruit (Passiflora spp.) [J]. | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (17) : 10138-10148 . |
| MLA | Fang, Ting et al. "A 224-bp Indel in the Promoter of PeMYB114 Accounts for Anthocyanin Accumulation of Skin in Passion Fruit (Passiflora spp.)" . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 72 . 17 (2024) : 10138-10148 . |
| APA | Fang, Ting , Wang, Mengzhen , He, Ruijie , Chen, Qiaowen , He, Dayi , Chen, Xuerong et al. A 224-bp Indel in the Promoter of PeMYB114 Accounts for Anthocyanin Accumulation of Skin in Passion Fruit (Passiflora spp.) . | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY , 2024 , 72 (17) , 10138-10148 . |
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Abstract ：
本申请公开了一种百香果“无需组织培养”的毛状根遗传转化方法,包括以下步骤:将待转的目的载体转入发根农杆菌的感受态细胞中,挑取阳性菌株经培养后提取菌体,利用培养基重悬所述菌体至OD值为0.6‑1.2,静置4h得菌悬液;将百香果切去根部后完全浸入所述菌悬液中真空浸润15‑30min;共培养:将浸润后的植物材料扦插于育苗盘中,移至人工气候室内培养,生成毛状细根。本发明只需要4‑6周即可获得大量转基因发根。本发明方法操作简单,一步法免组培方式大大减少了组培的繁琐流程;采用切根的幼苗作为植物材料不受时间限制,不受品种限制;具备操作简单、成本低、通量高、周期短等优点。
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| GB/T 7714 | 方庭 , 潘佳怡 , 曾黎辉 et al. 一种无需组织培养的发根农杆菌介导百香果毛状根诱导方法 : CN202410788158.3[P]. | 2024-06-18 . |
| MLA | 方庭 et al. "一种无需组织培养的发根农杆菌介导百香果毛状根诱导方法" : CN202410788158.3. | 2024-06-18 . |
| APA | 方庭 , 潘佳怡 , 曾黎辉 , 梁宇尧 . 一种无需组织培养的发根农杆菌介导百香果毛状根诱导方法 : CN202410788158.3. | 2024-06-18 . |
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Sucrose content is one of the important factors to determine longan fruit flavor quality. To gain deep insight of molecular mechanism on sucrose accumulation in longan, we conducted comparative transcriptomic analysis between low sucrose content longan cultivar 'Qingkebaoyuan' and high sucrose content cultivar 'Songfengben'. A total of 12,350 unique differentially expressed genes (DEGs) were detected across various development stages and different varieties, including hexokinase (HK) and sucrose-phosphate synthase (SPS), which are intricately linked to soluble sugar accumulation and metabolism. Weighted gene co-expression network analysis (WGCNA) identified magenta module, including DlSPS gene, was significantly positively correlated with sucrose content. Furthermore, transient expression unveiled DlSPS gene play crucial role in sucrose accumulation. Moreover, 5 transcription factors (MYB, ERF, bHLH, C2H2, and NAC) were potentially involved in DlSPS regulation. Our findings provide clues for sucrose metabolism, and lay the foundation for longan breeding in the future.
Keyword ：
interact network interact network longan longan sucrose sucrose sucrose-phosphate synthase sucrose-phosphate synthase transcription factor transcription factor
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| GB/T 7714 | Li, Yun , Ren, Rui , Pan, Ruoyun et al. Comparative transcriptome analysis identifies candidate genes related to sucrose accumulation in longan (Dimocarpus longan Lour.) pulp [J]. | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
| MLA | Li, Yun et al. "Comparative transcriptome analysis identifies candidate genes related to sucrose accumulation in longan (Dimocarpus longan Lour.) pulp" . | FRONTIERS IN PLANT SCIENCE 15 (2024) . |
| APA | Li, Yun , Ren, Rui , Pan, Ruoyun , Bao, Yuying , Xie, Tao , Zeng, Lihui et al. Comparative transcriptome analysis identifies candidate genes related to sucrose accumulation in longan (Dimocarpus longan Lour.) pulp . | FRONTIERS IN PLANT SCIENCE , 2024 , 15 . |
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本发明涉及果树繁殖领域，具体是一种百香果微小茎尖体内嫁接方法，包括以下步骤：（1）准备砧木及接穗；（2）微小茎尖体内嫁接方法：通过体视显微镜，在抗氧化溶液的配合下，用注射器针头作手术刀，先对砧木茎上做1×0.5mm的纵向矩形嫁接切口，再从接穗上切取小于1mm的茎尖并移至嫁接切口处定位，砧木去顶并固定茎尖后将嫁接苗移栽至基质中，完成微小茎尖体内嫁接；（3）嫁接后管理。本发明创新了微小茎尖体内嫁接技术，无需无菌条件，解决了微小茎尖固定不稳、易失水、难以与砧木充分结合等问题，从而实现小于1mm茎尖的体内嫁接，提高成活率，并达到脱毒的效果，为百香果脱毒苗繁育奠定基础。
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| GB/T 7714 | 曾黎辉 , 罗怡 , 李越 . 一种百香果微小茎尖体内嫁接方法 : CN202311607566.6[P]. | 2023-11-29 . |
| MLA | 曾黎辉 et al. "一种百香果微小茎尖体内嫁接方法" : CN202311607566.6. | 2023-11-29 . |
| APA | 曾黎辉 , 罗怡 , 李越 . 一种百香果微小茎尖体内嫁接方法 : CN202311607566.6. | 2023-11-29 . |
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