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The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress SCIE
期刊论文 | 2021 , 22 (1) | BMC GENOMICS
WoS CC Cited Count: 13
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BackgroundIn plants, Calcium (Ca2+) acts as a universal messenger in various signal transduction pathways, including responses to biotic and abiotic stresses and regulation of cellular and developmental processes. The Ca2+/cation antiporter (CaCA) superfamily proteins play vital roles in the transport of Ca2+ and/or other cations. However, the characteristics of these superfamily members in Saccharum and their evolutionary and functional implications have remained unclear.ResultsA total of 34 CaCA genes in Saccharum spontaneum, 5 CaCA genes in Saccharum spp. R570, and 14 CaCA genes in Sorghum bicolor were identified and characterized. These genes consisted of the H+/cation exchanger (CAX), cation/Ca2+ exchanger (CCX), EF-hand / CAX (EFCAX), and Mg2+/H+ exchanger (MHX) families, among which the CCX and EFCAX could be classified into three groups while the CAX could be divided into two groups. The exon/intron structures and motif compositions suggested that the members in the same group were highly conserved. Synteny analysis of CaCAs established their orthologous and paralogous relationships among the superfamily in S. spontaneum, R570, and S. bicolor. The results of protein-protein interactions indicated that these CaCA proteins had direct or indirect interactions. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis demonstrated that most members of Saccharum CaCA genes exhibited a similar expression pattern in response to hormonal (abscisic acid, ABA) treatment but played various roles in response to biotic (Sporisorium scitamineum) and abiotic (cold) stresses. Furthermore, ScCAX4, a gene encoding a cytoplasm, plasma membrane and nucleus positioning protein, was isolated from sugarcane. This gene was constitutively expressed in different sugarcane tissues and its expression was only induced at 3 and 6 h time points after ABA treatment, however was inhibited and indued in the whole process under cold and S. scitamineum stresses, respectively.ConclusionsThis study systematically conducted comparative analyses of CaCA superfamily genes among S. spontaneum, R570, and S. bicolor, delineating their sequence and structure characteristics, classification, evolutionary history, and putative functions. These results not only provided rich gene resources for exploring the molecular mechanism of the CaCA superfamily genes but also offered guidance and reference for research on other gene families in Saccharum.

Keyword :

Ca2+ Ca2+ cation antiporter (CaCA) superfamily cation antiporter (CaCA) superfamily Functional divergence Functional divergence Molecular evolution Molecular evolution Saccharum Saccharum Stress Stress Subcellular location Subcellular location

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GB/T 7714 Su, Weihua , Zhang, Chang , Wang, Dongjiao et al. The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress [J]. | BMC GENOMICS , 2021 , 22 (1) .
MLA Su, Weihua et al. "The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress" . | BMC GENOMICS 22 . 1 (2021) .
APA Su, Weihua , Zhang, Chang , Wang, Dongjiao , Ren, Yongjuan , Sun, Tingting , Feng, Jingfang et al. The CaCA superfamily genes in Saccharum: comparative analysis and their functional implications in response to biotic and abiotic stress . | BMC GENOMICS , 2021 , 22 (1) .
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镁营养对苦瓜生长发育及生理代谢的影响 CSCD
期刊论文 | 2021 , 42 (12) , 3545-3552 | 热带作物学报
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采用砂培的方式,以‘翠玉’苦瓜(Momordica charantia)品种为试验材料,研究不同镁素浓度(0、20、40、80、160 mg/L)处理下对苦瓜生长特性(叶片和根系形态、光合色素含量、碳氮代谢、生物量积累)和生理响应(渗透调节、膜伤害和抗氧化)的影响。结果表明:镁浓度为20~80 mg/L时可以降低苦瓜叶片膜伤害,增加叶片中可溶性糖、可溶性蛋白质、光合色素和谷胱甘肽(GSH)含量,增强叶片抗氧化酶活性,包括超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和单脱氢抗坏血酸还原酶(DHAR),降低叶片丙二醛(MDA)、脯氨酸(Pro)、过氧化氢(H_2O_2)含量和超氧阴离子(O_2~-)的产生速率及细胞膜透性,促进根系活力、光合作用和生物量积累。其中,40 mg/L镁处理对苦瓜的生长发育增效最明显,而缺镁(0 mg/L)和过量镁(160 mg/L)胁迫下,苦瓜叶片产生膜脂过氧化伤害、主根变短、侧根减少,苦瓜地上部和地下部生长均受到明显抑制,缺镁比镁过量的抑制作用更强。综上所述,缺镁和过量镁抑制苦瓜生长,而适量增施镁可有效提高苦瓜的生理活性,增强苦瓜抗逆性,促进苦瓜生长,40 mg/L为苦瓜栽培最适宜施镁浓度。

Keyword :

响应机制 响应机制 生理生化特性 生理生化特性 生长发育 生长发育 苦瓜 苦瓜

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GB/T 7714 尤垂淮 , 孙青慧 , 陈晟 et al. 镁营养对苦瓜生长发育及生理代谢的影响 [J]. | 热带作物学报 , 2021 , 42 (12) : 3545-3552 .
MLA 尤垂淮 et al. "镁营养对苦瓜生长发育及生理代谢的影响" . | 热带作物学报 42 . 12 (2021) : 3545-3552 .
APA 尤垂淮 , 孙青慧 , 陈晟 , 柯彦 , 林新萍 , 施木田 . 镁营养对苦瓜生长发育及生理代谢的影响 . | 热带作物学报 , 2021 , 42 (12) , 3545-3552 .
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镁营养对西瓜叶绿素荧光特性及生理代谢的影响 CSCD
期刊论文 | 2021 , 36 (11) , 1302-1314 | 福建农业学报
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【目的】探讨镁营养对西瓜(Citrullus lanatus)生长发育和生理代谢的影响,明确西瓜生长的适宜镁浓度范围。【方法】采用砂培法,对西瓜进行5个施镁质量浓度(0、24、48、96和192 mg·L~(-1))处理,并测定西瓜在不同施镁浓度下的生长特性(叶片和根系形态、生物量积累)、果实品质(维生素C、可溶性固形物、可溶性蛋白和可溶性糖含量)、叶绿素荧光特性和生理响应(渗透调节、膜伤害和抗氧化酶系统)。【结果】用24~96mg·L~(-1)的镁素可以降低西瓜叶片膜伤害,提高光系统Ⅱ(PSII)活性,增加叶片抗氧化物质谷胱甘肽(glutathione,GSH)和还原型抗坏血酸(reducedascorbicacid,AsA)含量,增强叶片抗氧化酶活性,包括过氧化物酶(peroxidase, POD)、超氧化物歧化酶(superoxide dismutase, SOD)、过氧化氢酶(catalase, CAT)和单脱氢抗坏血酸还原酶(monodehydroascorbate reductase,DHAR),降低叶片丙二醛(malondialdehyde,MDA)和脯氨酸(proline, Pro)含量以及细胞膜透性,促进AsA-GSH循环,增强光合作用,提高生物量积累,增加果实中维生素C、可溶性固形物、可溶性蛋白和可溶性糖含量。其中,48 mg·L~(-1)镁处理对西瓜的生长发育增效最明显,而缺镁(0 mg·L~(-1))和镁过量(192 mg·L~(-1))胁迫下,西瓜叶片产生膜脂过氧化伤害,根系变短,叶绿素荧光参数放氧复合体(OEC)、电子传递量子产额(φ_(Eo))、受体库容量(Sm)和单位面积反应中心数量(RC/CSo)降低,单位反应中心光能的吸收(ABS/RC)、耗散(DI0/RC)、捕获(TR0/RC)及Q_A还原速率(Mo)增加,叶片光合机构完整性被破坏,光合作用减弱,西瓜生长受到明显抑制,其中缺镁影响最严重。【结论】缺镁和镁过量处理降低西瓜PSII活性,抑制西瓜生长,而适量增施镁可有效提高西瓜的生理活性,PSII的结构和功能稳定,光合作用强,西瓜生长好,果实品质高,48 mg·L~(-1)为西瓜栽培最适宜施镁质量浓度。

Keyword :

叶绿素荧光 叶绿素荧光 响应机理 响应机理 生理生化指标 生理生化指标 西瓜 西瓜

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GB/T 7714 尤垂淮 , 林丽琳 , 陈晟 et al. 镁营养对西瓜叶绿素荧光特性及生理代谢的影响 [J]. | 福建农业学报 , 2021 , 36 (11) : 1302-1314 .
MLA 尤垂淮 et al. "镁营养对西瓜叶绿素荧光特性及生理代谢的影响" . | 福建农业学报 36 . 11 (2021) : 1302-1314 .
APA 尤垂淮 , 林丽琳 , 陈晟 , 吴宇芬 , 赵依杰 , 柯彦 et al. 镁营养对西瓜叶绿素荧光特性及生理代谢的影响 . | 福建农业学报 , 2021 , 36 (11) , 1302-1314 .
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一种蝴蝶兰专用氨基酸水溶固体肥料及其制备方法 incoPat ipsunlight
专利 | 2020-05-29 | CN202010475844.7
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本发明提供了一种蝴蝶兰专用氨基酸水溶固体肥料,按照质量分数含以下组分:复合氨基酸粉26%~32%,尿素6%~12%,磷酸二氢钾20%~28%,七水合硫酸镁18%~22%,七水合硫酸锌5%~12%,硼砂13%~20%,钼酸铵0.4%~0.8%。该肥料可促进蝴蝶兰生长,提高蝴蝶兰品质。

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GB/T 7714 兰思仁 , 施木田 , 何碧珠 et al. 一种蝴蝶兰专用氨基酸水溶固体肥料及其制备方法 : CN202010475844.7[P]. | 2020-05-29 .
MLA 兰思仁 et al. "一种蝴蝶兰专用氨基酸水溶固体肥料及其制备方法" : CN202010475844.7. | 2020-05-29 .
APA 兰思仁 , 施木田 , 何碧珠 , 马晓开 , 吴宇函 . 一种蝴蝶兰专用氨基酸水溶固体肥料及其制备方法 : CN202010475844.7. | 2020-05-29 .
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Improved Drought and Salt Tolerance in TransgenicNicotiana benthamianaby Overexpressing SugarcaneScSEC14pGene SCIE
期刊论文 | 2020 , 23 (2) , 326-335 | SUGAR TECH
WoS CC Cited Count: 2
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Plant phosphatidylinositol transfer proteins (PITPs) are involved in several cellular processes such as lipid metabolism, signal transduction, osmotic regulation, protein transport and stress response. In this study, the effects of sugarcanePITPgeneScSEC14p(accession no. MH899751) on drought and salt tolerance of transgenicNicotiana benthamianawere evaluated along with the underlying mechanisms. The results showed thatScSEC14pgene effectively improved drought and salt tolerance of transgenic lines ofN. benthamiana, as indicated by the increased germination rate and superoxide dismutase levels, as well as the decreased malondialdehyde level. In addition, the enhanced tolerance was closely related to the upregulation of stress-related genes and key genes in phosphatidylinositol pathway. It is revealed that the overexpression ofScSEC14pgene resulted in improved drought and salt tolerance of transgenic tobacco and also elucidated the underlying regulation mechanism byScSEC14pin sugarcane.

Keyword :

Drought tolerance Drought tolerance Osmotic stress Osmotic stress Phosphatidylinositol transfer proteins (PITPs) Phosphatidylinositol transfer proteins (PITPs) Salt tolerance Salt tolerance Transgenic tobacco Transgenic tobacco

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GB/T 7714 Ren, Yongjuan , Mao, Huaying , Su, Weihua et al. Improved Drought and Salt Tolerance in TransgenicNicotiana benthamianaby Overexpressing SugarcaneScSEC14pGene [J]. | SUGAR TECH , 2020 , 23 (2) : 326-335 .
MLA Ren, Yongjuan et al. "Improved Drought and Salt Tolerance in TransgenicNicotiana benthamianaby Overexpressing SugarcaneScSEC14pGene" . | SUGAR TECH 23 . 2 (2020) : 326-335 .
APA Ren, Yongjuan , Mao, Huaying , Su, Weihua , Su, Yachun , Zhang, Chang , Shi, Mutian et al. Improved Drought and Salt Tolerance in TransgenicNicotiana benthamianaby Overexpressing SugarcaneScSEC14pGene . | SUGAR TECH , 2020 , 23 (2) , 326-335 .
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硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响
期刊论文 | 2018 , 34 (04) , 60-64 | 中国农学通报
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为研究硼对不同类型西瓜活性氧代谢与抗氧化系统的影响,以籽瓜‘建籽’、杂交品种‘黑美人’和野生西瓜‘勇士’为材料,设0.00、0.05、0.20、0.60 mmol/L 4个硼浓度,测定3种类型西瓜O2·-、H_2O_2和MDA含量及SOD、POD和CAT活性。结果表明,3种类型西瓜的O2·-和H_2O_2产生速率在硼胁迫下均显著提高,且高硼胁迫的西瓜O2·-和H_2O_2产生速率都大于缺硼胁迫,并导致MDA的积累。硼胁迫使3种类型西瓜的POD活性上升,CAT活性下降。‘建籽’和‘黑美人’的SOD活性下降,‘勇士’SOD活性上升。硼胁迫引起西瓜活性氧爆发,并激活了西瓜叶片的抗氧化系统,但却不能完全抵抗硼胁迫导致的活性氧爆发,‘勇士’对硼胁迫的抗逆最强。

Keyword :

抗氧化系统 抗氧化系统 活性氧代谢 活性氧代谢 西瓜 西瓜

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GB/T 7714 陈晟 , 施木田 , 吴宇芬 et al. 硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响 [J]. | 中国农学通报 , 2018 , 34 (04) : 60-64 .
MLA 陈晟 et al. "硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响" . | 中国农学通报 34 . 04 (2018) : 60-64 .
APA 陈晟 , 施木田 , 吴宇芬 , 李永裕 . 硼胁迫对不同类型西瓜活性氧代谢与抗氧化系统的影响 . | 中国农学通报 , 2018 , 34 (04) , 60-64 .
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设施农业装备课程理论与实践教学模式改革方案及成效
期刊论文 | 2017 , (24) , 284,287 | 现代农业科技
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针对目前大学生学习中存在的被动记忆、缺乏热情、教条僵化等问题,本文通过教学模式改革,在理论教学中采用宏观引导、难点讲授、课堂汇报和提问答疑的新翻转课堂教学模式,在实践教学中尝试让学生"走出去",使其所学理论知识与一线生产实践接轨,以期全方位提高学生的综合能力。

Keyword :

实践教学 实践教学 成效 成效 教学改革 教学改革 理论教学 理论教学 设施农业装备 设施农业装备

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GB/T 7714 侯毛毛 , 施木田 , 申宝营 et al. 设施农业装备课程理论与实践教学模式改革方案及成效 [J]. | 现代农业科技 , 2017 , (24) : 284,287 .
MLA 侯毛毛 et al. "设施农业装备课程理论与实践教学模式改革方案及成效" . | 现代农业科技 24 (2017) : 284,287 .
APA 侯毛毛 , 施木田 , 申宝营 , 杨碧云 . 设施农业装备课程理论与实践教学模式改革方案及成效 . | 现代农业科技 , 2017 , (24) , 284,287 .
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硼水平对不同类型西瓜生长的影响
期刊论文 | 2016 , (02) , 43-47,102 | 中国果树
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以栽培西瓜品种‘黑美人’、籽用品种‘建籽’和野生品种‘勇士’为试材,设0、0.05、0.2、0.6 mmol/L H3BO34个浓度,其中0.05 mmol/L H3BO3为对照,研究硼胁迫对不同类型西瓜生长的影响,分析其硼效率差异。结果表明:缺硼胁迫(0 mmol/L H3BO3)对西瓜地上部有明显抑制作用,症状多位于顶端新叶;高硼胁迫(0.2、0.6 mmol/L H3BO3)严重危害西瓜地下部,其症状出现时间均较早,且多见底部老叶,逐步向上发展,阻碍其他部位的正常生长。缺硼与高硼胁迫均降低了西瓜株高、茎粗、根长,0、0.6 mmol/L H3BO3处理对西瓜株高、茎粗、根长的降低幅度较大。高硼胁迫对3种类型西瓜根系的生物产量影响较大,而缺硼胁迫下西瓜根系的生物产量降低幅度较小。‘黑美人’在缺硼胁迫和高硼胁迫下根系活力均出现下降,下降幅度较一致;‘建籽’‘勇士’在高硼胁迫下根系活力明显下降,且下降幅度远大于缺硼胁迫。不同类型西瓜存在着硼效率差异,籽用西瓜‘建籽’和野生西瓜‘勇士’硼效率较高,栽培西瓜‘黑美人’硼效率较低。

Keyword :

‘勇士’ ‘勇士’ ‘建籽’ ‘建籽’ 生物产量 生物产量 生长 生长 硼效率 硼效率 西瓜 西瓜 ‘黑美人’ ‘黑美人’

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GB/T 7714 陈晟 , 施木田 , 吴宇芬 et al. 硼水平对不同类型西瓜生长的影响 [J]. | 中国果树 , 2016 , (02) : 43-47,102 .
MLA 陈晟 et al. "硼水平对不同类型西瓜生长的影响" . | 中国果树 02 (2016) : 43-47,102 .
APA 陈晟 , 施木田 , 吴宇芬 , 李永裕 . 硼水平对不同类型西瓜生长的影响 . | 中国果树 , 2016 , (02) , 43-47,102 .
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“设施植物营养学”植物营养诊断实验教学新探索
期刊论文 | 2016 , 32 (10) , 209-211 | 中国园艺文摘
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《设施植物营养学》是一门具备较强理论性和实践性的课程,植物营养诊断实验课作为《设施植物营养学》重要的组成部分,起到衔接理论知识和实践活动的关键作用。为更好地实现教学相长,在植物营养诊断实验课传统的展示性讲授教学的基础上进行新的探索。通过教师创造条件、学生根据所学理论知识主动发现并解决问题的实验方法,增强学生自主学习的意识和能力,调动学生的学习兴趣,加深学生对专业理论知识的理解,促进学生的理论知识和实践知识更紧密结合。

Keyword :

实验 实验 探索 探索 改革 改革 自主学习 自主学习

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GB/T 7714 侯毛毛 , 施木田 . “设施植物营养学”植物营养诊断实验教学新探索 [J]. | 中国园艺文摘 , 2016 , 32 (10) : 209-211 .
MLA 侯毛毛 et al. "“设施植物营养学”植物营养诊断实验教学新探索" . | 中国园艺文摘 32 . 10 (2016) : 209-211 .
APA 侯毛毛 , 施木田 . “设施植物营养学”植物营养诊断实验教学新探索 . | 中国园艺文摘 , 2016 , 32 (10) , 209-211 .
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硼胁迫对不同基因型西瓜部分生理指标的影响
期刊论文 | 2016 , 32 (16) , 56-60 | 中国农学通报
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Abstract :

为了研究硼胁迫对不同基因型西瓜细胞壁、细胞膜以及碳氮代谢的影响,以籽瓜、‘黑美人’西瓜和野生西瓜为材料,设4个硼浓度,测定3种类型西瓜细胞膜透性、游离脯氨酸、可溶性糖、可溶性蛋白含量及PAL和PPO活性。结果表明:3种类型西瓜在硼胁迫下的PPO活性上升,细胞膜透性与游离脯氨酸含量上升,可溶性糖和可溶性蛋白含量有所减少。PAL活性在缺硼胁迫时下降,在高硼胁迫时应激上升。硼胁迫破坏了3种类型西瓜细胞壁结构,产生醌等大量次生产物损伤细胞膜,使细胞膜透性增强,细胞内离子浓度失衡脱水。进而阻碍了细胞正常功能运转,间接削弱西瓜碳代谢系统与氮代谢系统,减少了可溶性糖与可溶性蛋白质等代谢产物的生成。

Keyword :

碳氮代谢 碳氮代谢 细胞壁 细胞壁 细胞膜 细胞膜 西瓜 西瓜

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GB/T 7714 陈晟 , 施木田 , 吴宇芬 et al. 硼胁迫对不同基因型西瓜部分生理指标的影响 [J]. | 中国农学通报 , 2016 , 32 (16) : 56-60 .
MLA 陈晟 et al. "硼胁迫对不同基因型西瓜部分生理指标的影响" . | 中国农学通报 32 . 16 (2016) : 56-60 .
APA 陈晟 , 施木田 , 吴宇芬 , 李永裕 . 硼胁迫对不同基因型西瓜部分生理指标的影响 . | 中国农学通报 , 2016 , 32 (16) , 56-60 .
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