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学者姓名：文志丰
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【目的】克隆刺葡萄类钙调蛋白基因VdCML8基因及其启动子序列，并对VdCML8基因进行表达分析，对其启动子进行转录活性测定，为深入探究该基因在葡萄抗炭疽病中的生物学功能提供理论参考。【方法】以刺葡萄紫秋为材料，采用RT-PCR技术克隆VdCML8基因及其启动子序列，对VdCML8蛋白的理化性质和二级结构进行生物信息学分析，并采用实时荧光定量PCR检测刺葡萄紫秋和欧洲葡萄红地球CML8基因在接种胶孢炭疽菌及外施水杨酸（SA）和茉莉酸（JA）处理后的表达特征，通过构建β-葡萄糖苷酶（GUS）融合载体转化烟草进行转录活性检测。【结果】VdCML8基因的开放阅读框（ORF）长度为450bp，编码149个氨基酸残基，具有EF-hand结构域，其二级结构中α-螺旋占65.10%，延伸链占4.70%，无规则卷曲占20.13%,β-转角占10.07%，该蛋白定位于细胞膜中。由系统发育进化树可知，刺葡萄VdCML8蛋白与欧洲葡萄VvCML8和河岸葡萄Vr CML8的亲缘关系较近。VdCML8基因的启动子序列（p VdCML8）长度为1050 bp，除含有大量的CAAT-box和TATA-box外，还含有一些光响应元件（L-box、chs-CMALa和TCT-motif）、脱落酸（ABA）响应元件（ABRE）、厌氧诱导响应元件（ARE）、防御和应激元件（TC-richrepeats）、伤害响应元件（WUN-motif）等。构建pVdCML8的瞬时表达载体pVdCML8::GUS，瞬时转化烟草后发现pVdCML8具有转录活性，且能驱动VdCML8基因表达。在接种胶孢炭疽菌后，刺葡萄紫秋VdCML8基因和欧洲葡萄红地球VvCML8基因表达均上调，均在接种后12 h达峰值，二者的相对表达量是对照组（清水处理）的22.08和9.30倍。SA处理3 h时，VdCML8基因的相对表达量是对照组的7.68倍，是VvCML8基因的2.76倍。JA处理6 h时，VdCML8基因达峰值，是对照组的22.25倍，是VvCML8基因的9.04倍。【结论】VdCML8基因是SA和JA信号途径的下游调控基因，SA和JA可诱导其高效表达，参与葡萄炭疽病响应过程，对提高植株抗病性具有一定作用。
Keyword ：
VdCML8 VdCML8 刺葡萄 刺葡萄 启动子 启动子 胶孢炭疽病 胶孢炭疽病 表达分析 表达分析 转录活性 转录活性
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| GB/T 7714 | 段枫枫 , 陈漫影 , 雷天赐 et al. 刺葡萄类钙调蛋白基因VdCML8克隆与表达分析及其启动子转录活性测定 [J]. | 南方农业学报 , 2024 , 55 (08) : 2225-2236 . |
| MLA | 段枫枫 et al. "刺葡萄类钙调蛋白基因VdCML8克隆与表达分析及其启动子转录活性测定" . | 南方农业学报 55 . 08 (2024) : 2225-2236 . |
| APA | 段枫枫 , 陈漫影 , 雷天赐 , 张梦琪 , 文志丰 . 刺葡萄类钙调蛋白基因VdCML8克隆与表达分析及其启动子转录活性测定 . | 南方农业学报 , 2024 , 55 (08) , 2225-2236 . |
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Anthracnose, caused by Colletotrichum spp. is one of the most devastating disease of strawberry. Identification of strawberry resistance to anthracnose has been a priority in strawberry breeding programs. Here, a total of 34 strawberry genotypes were evaluated for resistance to Colletotrichum gloeosporioides using detached leaf assay. Following C. gloeosporioides inoculation, the activities of defense enzymes such as catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), superoxide anion (center dot O-2(-)) accumulation, and expression of pathogenesis-related 1 (PR1) and plant defending 1.2 (PDF 1.2) genes were examined. Two genotypes were resistant (R), seven moderately resistant (MR), thirteen susceptible (S), and twelve highly susceptible (HS). Fragaria nilgerrensis Schlech. and F. mandschurica were resistant to C. gloeosporioides, while F. vesca 'Hawaii 4' and Fragaria x ananassa cv 'Miaoxiang No. 3' were highly sensitive to the pathogen. Furthermore, the leaves of F. nilgerrensis, displayed more center dot O-2(-) production and cell death than 'Miaoxiang No. 3' following pathogen inoculation. The activities of defense enzymes such as CAT, POD, and SOD in F. nilgerrensis and F. mandschurica were higher than in "Hawaii 4" and 'Miaoxiang No. 3'. Following inoculation, PR1, PR5, PDF1.2, and coronatine insensitive 1 gene showed high expression levels in F. nilgerrensis, F. mandschurica, 'Hawaii 4', and 'Miaoxiang No. 3'. This work provides insights into the response of different strawberry genotypes to C. gloeosporioides infection in and will help breeders in selecting strawberry germplasm for disease-resistant breeding programs.
Keyword ：
Anthracnose Anthracnose Colletotrichum gloeosporioides Colletotrichum gloeosporioides Resistance Resistance ROS ROS Strawberry Strawberry
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| GB/T 7714 | Shi, Yaqian , Shen, Yaru , Bai, Jinhui et al. Detached leaf assay-based screening of anthracnose resistant wild strawberry genotypes [J]. | TROPICAL PLANT PATHOLOGY , 2024 , 49 (6) : 876-885 . |
| MLA | Shi, Yaqian et al. "Detached leaf assay-based screening of anthracnose resistant wild strawberry genotypes" . | TROPICAL PLANT PATHOLOGY 49 . 6 (2024) : 876-885 . |
| APA | Shi, Yaqian , Shen, Yaru , Bai, Jinhui , Ahmad, Bilal , Rahman, Mati Ur , Chen, Qingxi et al. Detached leaf assay-based screening of anthracnose resistant wild strawberry genotypes . | TROPICAL PLANT PATHOLOGY , 2024 , 49 (6) , 876-885 . |
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Background DIR (Dirigent) proteins play important roles in the biosynthesis of lignin and lignans and are involved in various processes such as plant growth, development, and stress responses. However, there is less information about VvDIR proteins in grapevine (Vitis vinifera L). Results In this study, we used bioinformatics methods to identify members of the DIR gene family in grapevine and identified 18 VvDIR genes in grapevine. These genes were classified into 5 subfamilies based on phylogenetic analysis. In promoter analysis, various plant hormones, stress, and light-responsive cis-elements were detected. Expression profiling of all genes following Colletotrichum gloeosporioides infection and phytohormones (salicylic acid (SA) and jasmonic acid (JA)) application suggested significant upregulation of 17 and 6 VvDIR genes, respectively. Further, we overexpressed the VvDIR4 gene in Arabidopsis thaliana and grapes for functional analysis. Ectopic expression of VvDIR4 in A. thaliana and transient expression in grapes increased resistance against C. gloeosporioides and C. higginsianum, respectively. Phenotypic observations showed small disease lesions in transgenic plants. Further, the expression patterns of genes having presumed roles in SA and JA signaling pathways were also influenced. Lignin contents were measured before and after C. higginsianum infection; the transgenic A. thaliana lines showed higher lignin content than wild-type, and a significant increase was observed after C. higginsianum infection. Conclusions Based on the findings, we surmise that VvDIR4 is involved in hormonal and lignin synthesis pathways which regulate resistance against anthracnose. Our study provides novel insights into the function of VvDIR genes and new candidate genes for grapevine disease resistance breeding programs.
Keyword ：
Colletotrichum gloeosporioides Colletotrichum gloeosporioides Dirigent gene Dirigent gene Disease resistance Disease resistance Vitis vinifera L. Vitis vinifera L. VvDIR4 VvDIR4
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| GB/T 7714 | Zhang, Qimeng , Luo, Ning , Dai, Xicheng et al. Ectopic and transient expression of VvDIR4 gene in Arabidopsis and grapes enhances resistance to anthracnose via affecting hormone signaling pathways and lignin production [J]. | BMC GENOMICS , 2024 , 25 (1) . |
| MLA | Zhang, Qimeng et al. "Ectopic and transient expression of VvDIR4 gene in Arabidopsis and grapes enhances resistance to anthracnose via affecting hormone signaling pathways and lignin production" . | BMC GENOMICS 25 . 1 (2024) . |
| APA | Zhang, Qimeng , Luo, Ning , Dai, Xicheng , Lin, Jinhui , Ahmad, Bilal , Chen, Qingxi et al. Ectopic and transient expression of VvDIR4 gene in Arabidopsis and grapes enhances resistance to anthracnose via affecting hormone signaling pathways and lignin production . | BMC GENOMICS , 2024 , 25 (1) . |
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The PR10 protein (pathogenesis-related protein 10) represents a distinct branch within the broader PR protein family, known for its inducible expression in response to various fungal pathogens, thereby contributing to the plant's defense mechanisms. This study identified and characterized 28 FvPR10 genes from the Fragaria vesca (F. vesca) genome. Phylogenetic analysis classified these genes into 4 subfamilies (PR10-I, II, III, and IV). Comprehensive bioinformatics analyses were conducted, encompassing evaluations of physicochemical properties, chromosomal localization, exon-intron distributions, structural domain arrangements, and subcellular localization patterns of the FvPR10 genes. Our findings revealed tissue-specific expression patterns for the FvPR10 genes, with their transcriptional activation observed in response to stress conditions. Notably, following inoculation with Colletotrichum gloeosporioides, several FvPR10 genes significantly elevated expression levels, peaking at 6 h post-inoculation (hpi). Among these genes, FvPR10-7 demonstrated particularly robust expression, especially when treated with salicylic acid (SA) or jasmonic acid (JA), implicating its pivotal role in defense responses. Moreover, the overexpression of FvPR10-7 in Arabidopsis thaliana (A. thaliana) conferred heightened resistance against Colletotrichum higginsianum, accompanied by upregulation of defense-related genes associated with the SA pathways. These results collectively suggest that FvPR10-7 orchestrates plant defense responses by modulating SA signaling pathways, thereby underscoring its significance in plant immunity against fungal pathogens.
Keyword ：
Colletotrichum gloeosporioides Colletotrichum gloeosporioides Expression Expression Fragaria vesca Fragaria vesca FvPR10 gene FvPR10 gene
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| GB/T 7714 | Luo, Ning , Zhang, Qimeng , Sun, Yingbo et al. Identification and expression of the FvPR10 gene family in strawberry (Fragaria vesca) and functional characterization of FvPR10-7 [J]. | PLANT GROWTH REGULATION , 2024 , 105 (1) : 187-202 . |
| MLA | Luo, Ning et al. "Identification and expression of the FvPR10 gene family in strawberry (Fragaria vesca) and functional characterization of FvPR10-7" . | PLANT GROWTH REGULATION 105 . 1 (2024) : 187-202 . |
| APA | Luo, Ning , Zhang, Qimeng , Sun, Yingbo , Chen, Qingxi , Wen, Zhifeng . Identification and expression of the FvPR10 gene family in strawberry (Fragaria vesca) and functional characterization of FvPR10-7 . | PLANT GROWTH REGULATION , 2024 , 105 (1) , 187-202 . |
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Plant chitinases (EC 3.2.1.14) are pathogenesis-related (PR) proteins and are well studied in many plant species. However, little is known about the genomic organization and expression of chitinase genes in strawberries (Fragaria vesca). Here, 23 FvChi genes were identified in the genome of strawberry (F. vesca) and divided into GH18 and GH19 subfamilies based on phylogenetic relationships. A detailed bioinformatics analysis of the FvChi genes was performed, including gene physicochemical properties, chromosomal location, exon-intron distribution, domain arrangement, and subcellular localization. Twenty-two FvChi genes showed upregulation after Colletotrichum gloeosporioides infection. Following the exogenous application of SA, FvChi-3, 4, and 5 showed significant changes in expression. The ectopic expression of FvChi-14 in Arabidopsis thaliana increased resistance to C. higginsianum via controlling the SA and JA signaling pathway genes (AtPR1, AtICS1, AtPDF1.2, and AtLOX3). The FvChi-14 protein location was predicted in the cell wall or extracellular matrix. We speculate that FvChi-14 is involved in disease resistance by regulating the SA and JA signaling pathways. The findings of this study provide a theoretical reference for the functional studies of FvChi genes and new candidates for strawberry stress resistance breeding programs.
Keyword ：
biotic stress biotic stress evolutionary history evolutionary history genome genome pathogen pathogen PR3 PR3
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| GB/T 7714 | He, Tiannan , Fan, Jianshuai , Jiao, Gaozhen et al. Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14 [J]. | PLANTS-BASEL , 2023 , 12 (7) . |
| MLA | He, Tiannan et al. "Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14" . | PLANTS-BASEL 12 . 7 (2023) . |
| APA | He, Tiannan , Fan, Jianshuai , Jiao, Gaozhen , Liu, Yuhan , Zhang, Qimeng , Luo, Ning et al. Bioinformatics and Expression Analysis of the Chitinase Genes in Strawberry (Fragaria vesca) and Functional Study of FvChi-14 . | PLANTS-BASEL , 2023 , 12 (7) . |
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The Trihelix is a plant-specific transcription factor family and has critical roles in plant growth and development and stress resistance. There is less information about Trihelix transcription factor genes and their potential functions in strawberries (Fragaria vesca). In the present study, we performed a detailed bioinformatics analysis of the Trihelix family in strawberries including physicochemical properties, chromosomal location, exon-intron distribution, domain arrangement, and subcellular localization. Thirty Trihelix family members were identified and divided into five subfamilies. The expression of FvTrihelix genes in different tissues/organs, i.e., root, stolon, leaf, flower, and fruit, was measured in strawberries after infection with Colletotrichum. gloeosporioides and foliar applications of salicylic acid (SA) and jasmonic acid (JA). Most of the genes showed differential expression responses following C. gloeosporioides infection and hormone treatments (SA and JA), suggesting critical roles in disease resistance and hormonal signaling pathways. As anticipated, the ectopic expression of FvTrihelix6 in Arabidopsis thaliana increased resistance against Colletotrichum. higginsianum infection. FvTrihelix6 protein was localized in the nucleus. We surmise that FvTrihelix6 enhances resistance against pathogens through the SA and JA signaling pathways. This study provides novel insights into the strawberry Trihelix transcription factor genes and new candidates for disease-resistance breeding of strawberries.
Keyword ：
Colletotrichum gloeosporioides Colletotrichum gloeosporioides disease-resistance verification disease-resistance verification strawberry (Fragaria vesca) strawberry (Fragaria vesca) trihelix transcription factor trihelix transcription factor
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| GB/T 7714 | Fan, Jianshuai , Jiang, Fan , Sun, Hongyuan et al. Expression Analysis of Trihelix Transcription Factor Family in Strawberries and Functional Characterization of FvTrihelix6 [J]. | HORTICULTURAE , 2023 , 9 (6) . |
| MLA | Fan, Jianshuai et al. "Expression Analysis of Trihelix Transcription Factor Family in Strawberries and Functional Characterization of FvTrihelix6" . | HORTICULTURAE 9 . 6 (2023) . |
| APA | Fan, Jianshuai , Jiang, Fan , Sun, Hongyuan , He, Tiannan , Liu, Yuhan , Jiao, Gaozhen et al. Expression Analysis of Trihelix Transcription Factor Family in Strawberries and Functional Characterization of FvTrihelix6 . | HORTICULTURAE , 2023 , 9 (6) . |
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Dirigent proteins (DIR) are thought to have important roles in lignans or lignin-like molecules production, defensive responses, secondary metabolism, and disease resistance in plants. There is a lack of information about the DIR genes in strawberries (Fragaria vesca). In this study, 33 FvDIR genes were identified and divided into four subfamilies based on the phylogenetic analysis. A detailed bioinformatic analysis of FvDIR genes was performed including physiochemical properties of genes, chromosomal locations, exon-intron distribution, motif arrangements, in silico analysis of cis-elements, and protein localization. All the genes were unevenly distributed on 7 chromosomes. The structure and chromosomal distribution of FvDIR genes were systematically analyzed. Most of the genes showed conserved motif and domain composition within the same subfamily. According to exon-intron analysis, 85% of FvDIR genes have no intron. Evolutionary history analysis showed that tandem duplication (six sets) has played more roles in the expansion of gene family than segmental duplication (one set). In silico analysis of cis-elements suggested that most FvDIR genes may function in response to light, hormones, and environmental stresses. Furthermore, we analyzed the expression of FvDIR genes in different plants parts (roots, stolons, leaves, flowers, and fruits), against Colletotrichum gloeosporioides inoculation, and hormone (SA and JA) treatments. The majority of the genes displayed tissue-specific expression patterns, suggesting their roles in plant growth and development. Some of the genes showed differential expression against C. gloeosporioides inoculation and hormone (SA and JA) treatments, signifying their critical roles in plant response to pathogen and hormone treatments. Moreover, ectopic expression of FvDIR13 (Acc. NO. MZ318691) in Arabidopsis thaliana exhibited enhanced resistance to C. higginsianum, increase in lignin contents, and change in expression of genes having presumed roles in JA and SA signaling pathways. We surmise that FvDIR13 can promote lignin synthesis, and has roles in disease resistance via regulating JA and SA responsive genes. Our findings give a new foundation for understanding the functions of FvDIR genes and provide preliminary information about the roles of FvDIR13 in disease resistance.
Keyword ：
Colletotrichum gloeosporioides Colletotrichum gloeosporioides Dirigent gene Dirigent gene Expression analysis Expression analysis Strawberry (Fragaria vesca) Strawberry (Fragaria vesca)
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| GB/T 7714 | Shi, Yaqian , Shen, Yaru , Ahmad, Bilal et al. Genome-wide identification and expression analysis of dirigent gene family in strawberry (Fragaria vesca) and functional characterization of FvDIR13 [J]. | SCIENTIA HORTICULTURAE , 2022 , 297 . |
| MLA | Shi, Yaqian et al. "Genome-wide identification and expression analysis of dirigent gene family in strawberry (Fragaria vesca) and functional characterization of FvDIR13" . | SCIENTIA HORTICULTURAE 297 (2022) . |
| APA | Shi, Yaqian , Shen, Yaru , Ahmad, Bilal , Yao, Liping , He, Tiannan , Fan, Jianshuai et al. Genome-wide identification and expression analysis of dirigent gene family in strawberry (Fragaria vesca) and functional characterization of FvDIR13 . | SCIENTIA HORTICULTURAE , 2022 , 297 . |
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Insulators in vertebrates play a role in genome architecture and orchestrate temporo-spatial enhancer-promoter interactions. In plants, insulators and their associated binding factors have not been documented as of yet, largely as a result of a lack of characterized insulators. In this study, we took a comprehensive strategy to identify and validate the enhancer-blocking insulator CW198. We show that a 1.08-kb CW198 fragment from Arabidopsis can, when interposed between an enhancer and a promoter, efficiently abrogate the activation function of both constitutive and floral organ-specific enhancers in transgenic Arabidopsis and tobacco plants. In plants, both transcriptional crosstalk and spreading of histone modifications were rarely detectable across CW198, which resembles the insulation property observed across the CTCF insulator in the mammalian genome. Taken together, our findings support that CW198 acts as an enhancer-blocking insulator in both Arabidopsis and tobacco. The significance of the present findings and their relevance to the mitigation of mutual interference between enhancers and promoters, as well as multiple promoters in transgenes, is discussed.
Keyword ：
High-throughput screening High-throughput screening Insulator Insulator Promoter-promoter interaction Promoter-promoter interaction Transgenic plants Transgenic plants
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| GB/T 7714 | Jiang, Li , Liu, Yue , Wen, Zhifeng et al. CW198 acts as a genetic insulator to block enhancer-promoter interaction in plants [J]. | TRANSGENIC RESEARCH , 2022 , 31 (6) : 647-660 . |
| MLA | Jiang, Li et al. "CW198 acts as a genetic insulator to block enhancer-promoter interaction in plants" . | TRANSGENIC RESEARCH 31 . 6 (2022) : 647-660 . |
| APA | Jiang, Li , Liu, Yue , Wen, Zhifeng , Yang, Yingjun , Singer, Stacy D. , Bennett, Dennis et al. CW198 acts as a genetic insulator to block enhancer-promoter interaction in plants . | TRANSGENIC RESEARCH , 2022 , 31 (6) , 647-660 . |
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探索黄毛草莓FnFBOX1参与草莓胶孢炭疽菌(Colletotrichum gloeosporioides)侵染过程中的抗病反应和pFnFBOX1启动子的转录活性，为研究FnFBOX1抗炭疽病功能奠定基础。以黄毛草莓(Fragaria nilgerrensis Schidl.)为研究对象，通过RT-PCR技术克隆FnFBOX1的cDNA序列。通过氨基酸序列比对、进化树分析、亚细胞定位、启动子克隆并构建瞬时表达载体、组织特异性表达分析，最后用荧光定量分析了抗病的黄毛草莓和感病的‘妙香 3’草莓在水杨酸(SA)和胶孢炭疽菌胁迫下FnFBOX1的表达水平变化。结果表明，FnFBOX1的ORF全长为1 227 bp，编码408个氨基酸。氨基酸序列比对和进化树分析发现FnFBOX1与森林草莓(Fragaria vesca)FvFBOX1同源性最高。亚细胞定位结果显示FnFBOX1定位于细胞核。克隆的FnFBOX1启动子长度为821 bp，构建pFnFBOX1的瞬时表达载体pFnFBOX1∷GUS。瞬时转化烟草后对其进行组织化学染色分析和GUS活性测定，结果表明，pFnFBOX1具有驱动下游基因转录的活性，且胶孢炭疽菌和SA可促进FnFBOX1启动子启动的GUS活性的提高。荧光定量PCR分析表明，FnFBOX1在黄毛草莓的不同组织中均有表达。抗病黄毛草莓和感病‘妙香 3’草莓均进行接种胶孢炭疽菌和喷施SA处理，结果显示，黄毛草莓接种胶孢炭疽菌12 h后FnFBOX1表达量达到最高，是0 h的6.3倍；SA处理24 h时，其表达量达到最高，是0 h的7.9倍。‘妙香 3’草莓也响应胶孢炭疽菌和SA处理，但FBOX1表达量比黄毛草莓低。
Keyword ：
FnFBOX1 FnFBOX1 启动子 启动子 炭疽病 炭疽病 表达分析 表达分析 黄毛草莓 黄毛草莓
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| GB/T 7714 | 时雅倩 , 申亚茹 , 陈漫影 et al. 黄毛草莓F-box蛋白基因FnFBOX1及其启动子的克隆和表达分析 [J]. | 生物技术通报 , 2022 , 38 (02) : 44-56 . |
| MLA | 时雅倩 et al. "黄毛草莓F-box蛋白基因FnFBOX1及其启动子的克隆和表达分析" . | 生物技术通报 38 . 02 (2022) : 44-56 . |
| APA | 时雅倩 , 申亚茹 , 陈漫影 , 何淑敏 , 刘予涵 , 何天楠 et al. 黄毛草莓F-box蛋白基因FnFBOX1及其启动子的克隆和表达分析 . | 生物技术通报 , 2022 , 38 (02) , 44-56 . |
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Insulators characterized in Drosophila and mammals have been shown to play a key role in the restriction of promiscuous enhancer-promoter interactions, as well as reshaping the topological landscape of chromosomes. Yet the role of insulators in plants remains poorly understood, in large part because of a lack of well-characterized insulators and binding factor(s). In this study, we isolated a 1.2-kb RS2-9 insulator from the Oryza sativa (rice) genome that can, when interposed between an enhancer and promoter, efficiently block the activation function of both constitutive and floral organ-specific enhancers in transgenic Arabidopsis and Nicotiana tabacum (tobacco). In the rice genome, the genes flanking RS2-9 exhibit an absence of mutual transcriptional interactions, as well as a lack of histone modification spread. We further determined that O. sativa Homeobox 1 (OSH1) bound two regions of RS2-9, as well as over 50 000 additional sites in the rice genome, the majority of which resided in intergenic regions. Mutation of one of the two OSH1-binding sites in RS2-9 impaired insulation activity by up to 60%, whereas the mutation of both binding sites virtually abolished insulator function. We also demonstrated that OSH1 binding sites were associated with 72% of the boundaries of topologically associated domains (TADs) identified in the rice genome, which is comparable to the 77% of TAD boundaries bound by the insulator CCCTC-binding factor (CTCF) in mammals. Taken together, our findings indicate that OSH1-RS2-9 acts as a true insulator in plants, and highlight a potential role for OSH1 in gene insulation and topological organization in plant genomes.
Keyword ：
enhancer-promoter interaction enhancer-promoter interaction high-throughput screening high-throughput screening insulator and binding factor insulator and binding factor topologically associated domains (TADs) topologically associated domains (TADs) transgenic plants transgenic plants
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| GB/T 7714 | Liu, Huawei , Jiang, Li , Wen, Zhifeng et al. Rice RS2-9, which is bound by transcription factor OSH1, blocks enhancer-promoter interactions in plants [J]. | PLANT JOURNAL , 2021 , 109 (3) : 541-554 . |
| MLA | Liu, Huawei et al. "Rice RS2-9, which is bound by transcription factor OSH1, blocks enhancer-promoter interactions in plants" . | PLANT JOURNAL 109 . 3 (2021) : 541-554 . |
| APA | Liu, Huawei , Jiang, Li , Wen, Zhifeng , Yang, Yingjun , Singer, Stacy D. , Bennett, Dennis et al. Rice RS2-9, which is bound by transcription factor OSH1, blocks enhancer-promoter interactions in plants . | PLANT JOURNAL , 2021 , 109 (3) , 541-554 . |
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