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学者姓名:赖钟雄

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< Page ,Total 94 >
The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway SCIE
期刊论文 | 2025 , 223 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
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The basic-leucine zipper (bZIP) transcription factor (TF), which is abundant and highly conserved in eukaryotes, mainly participates in plant growth, development and adverse stress processes. However, the regulatory mechanisms of bZIP TFs under heat stress in Dimocarpus longan remain unclear. In this study, we reported a nuclear-localized bZIP TF, DlbZIP20, which was confirmed to be a positive regulator of thermotolerance in D. longan. It was identified as a strong interplay with hormone signalling pathways and oxidoreductase-related families. The transient overexpression DlbZIP20 could improve the thermotolerance of D. longan embryogenic callus (EC) and reduce the damage of cells under heat stress. Overexpression of DlbZIP20 in hairy roots significantly promoted roots elongation and photosystem II (PS II) responses in leaves, and significantly increased SOD and POD activities, promoted H2O2 scavenging, improved thermotolerance of D. longan by maintaining ROS homeostasis under heat stress. In addition, overexpression of DlbZIP20 increased endogenous MeJA content under heat stress. MeJA hormone synthesis genes DlMYC2 and DlCOI1 were up-regulated and DlJAZ3 was down-regulated in expression. This signalling pathway may play a role in the regulation of thermotolerance in D. longan. These results will provide a new insight into the molecular function of bZIP20 TF in response to heat stress in D. longan.

Keyword :

Abiotic stress responses Abiotic stress responses Antioxidant system Antioxidant system Hairy root culture Hairy root culture Plant hormone interactions Plant hormone interactions

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GB/T 7714 Zhai, Tingkai , Guo, Yueru , Yang, Mengmeng et al. The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 .
MLA Zhai, Tingkai et al. "The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 223 (2025) .
APA Zhai, Tingkai , Guo, Yueru , Yang, Mengmeng , Zhang, Xueying , Lin, Yiyao , Cai, Danfeng et al. The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 .
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Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua SCIE
期刊论文 | 2025 , 230 | INDUSTRIAL CROPS AND PRODUCTS
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Polygonatum cyrtonema Hua (P. cyrtonema), a traditional medicinal herb, is renowned for its high metabolite content. Previous studies have found that APETALA2/Ethylene Responsive Factor (AP2/ERF) plays a vital role in the metabolisms of P. cyrtonema, while the regulatory mechanism of PcAP2/ERFs in the flavonoid accumulation remains unclear. A total of 53 PcAP2/ERFs were identified from the full-length transcriptome. The expression analysis of PcAP2/ERFs in different age section rhizomes and different tissue sections showed that PcERF114 was associated with flavonoid accumulation and located in the nucleus. The flavonoid content was significantly upregulated with the transient overexpression of PcERF114, and significantly down-regulated with virus-induced gene silencing of PcERF114 in the P. cyrtonema leaves. Meanwhile, an integrative analysis of transcriptome and metabolome revealed that differentially expressed genes and differentially accumulated metabolites of transgenic leaves were closely related to the flavonoid biosynthesis pathway, and PcCHS1 was identified as a flavonoid biosynthesis-related key gene by WGCNA, qRT-PCR, and KEGG analysis. Overexpression of PcERF114 in the stable transgenic hairy roots and transiently transformed rhizomes increased the PcCHS1 expression, and the transient co-transformation of overexpressing PcERF114 and PcCHS1 promoted flavonoid accumulation, suggesting that PcERF114 may affect the expression of PcCHS1, thereby promoting the flavonoid accumulation of P. cyrtonema. The results provided a reference for further functional studies of secondary metabolism biosynthesis-related genes in medicinal plants.

Keyword :

AP2/ERF AP2/ERF ERF114 ERF114 Flavonoids Flavonoids Metabolome Metabolome Polygonatum cyrtonema Hua Polygonatum cyrtonema Hua Transcriptome Transcriptome

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GB/T 7714 Wan, Kui , Yang, Fengjie , Nan, Yanlong et al. Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 230 .
MLA Wan, Kui et al. "Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua" . | INDUSTRIAL CROPS AND PRODUCTS 230 (2025) .
APA Wan, Kui , Yang, Fengjie , Nan, Yanlong , Zhang, Shuting , Huang, Zhixia , Chen, Yukun et al. Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 230 .
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Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia' SCIE
期刊论文 | 2025 , 11 (1) | HORTICULTURAE
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Dormancy release is an important process for improving the quality of cut-flower lily production and promoting the factory production of lily bulbs. However, the regulatory mechanisms of microRNAs (miRNAs) and their target genes during the dormancy release of lily remain elusive. Anatomy, transcriptomic, molecular biology, and transient transformation techniques involving subcellular localization were applied in our study. There were significant results showing that 0.1 mM riboflavin promoted dormancy release and floral bud differentiation and influenced the flowering time of the Lilium 'Siberia'. Moreover, some differentially expressed miRNAs and their targets (miR395-y: LoAPS1, miR529-z: LoSPL14, miR396-y: LoCFDP1, miR1863-z: LoFBA3, miR399-y: LoDIT1, and miR11525-z: Lopgm) were identified and predicted. Exogenous riboflavin may activate primary metabolic processes and promote dormancy release in Lilium 'Siberia' bulbs. Furthermore, riboflavin upregulated genes related to the riboflavin pathway, H3K4me3 methylation, dormancy control, and the flowering pathway and downregulated dormancy maintenance genes. Moreover, riboflavin promoted endogenous riboflavin and acetyl-CoA accumulation. LoPurple acid phosphatase17 (LoPAP17), a pivotal gene of the riboflavin metabolism pathway, was subsequently cloned. LoPAP17 was most closely related to the orthologous genes of Acorus calamus, Asparagus officinalis, and Musa acuminata. The LoPAP17 protein was subcellularly located in the nucleus. Our study revealed that miRNAs and their target genes might regulate the primary metabolic pathway, promote the accumulation of endogenous riboflavin and acetyl-CoA, and affect protein acetylation during the riboflavin-promoted release of dormancy and flower bud differentiation in the Lilium Oriental hybrid 'Siberia'.

Keyword :

dormancy release dormancy release Lilium Oriental hybrid 'Siberia' Lilium Oriental hybrid 'Siberia' miRNA miRNA riboflavin riboflavin transcriptomics transcriptomics

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GB/T 7714 Xu, Xiaoping , Yang, Chenglong , Zheng, Yiping et al. Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia' [J]. | HORTICULTURAE , 2025 , 11 (1) .
MLA Xu, Xiaoping et al. "Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia'" . | HORTICULTURAE 11 . 1 (2025) .
APA Xu, Xiaoping , Yang, Chenglong , Zheng, Yiping , Guo, Wenjie , Lai, Zhongxiong , Fang, Shaozhong . Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia' . | HORTICULTURAE , 2025 , 11 (1) .
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Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress SCIE
期刊论文 | 2025 , 26 (7) | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
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Banana (Musa spp.) is susceptible to low-temperature stress and other environmental stresses, which can hinder the growth and development. Succinic semialdehyde dehydrogenase (SSADH) is critical for GABA biosynthesis and plays a crucial role in plants. However, the SSADH genes of bananas have not been studied. This study found 19 MaSSADHs, 18 MbSSADHs, and 18 MiSSADHs from the banana genome. According to the phylogenetic tree, these genes can be categorized into five branches. This study cloned the MaSSADH1-14 from banana. The subcellular localization assays of MaSSADH1-14 in tobacco leaves confirmed that the presence of SSADH was not only localized mitochondrion but also localized chloroplast. The cis-elements of the SSADH gene family are related to the potential regulation of the banana SSADH gene family; their involvement in diverse stress responses. Transcriptomic data was utilized to examine the effect of MaSSADH genes under cold stress in bananas. The results of RT-qPCR were consistent with transcriptome data. These results showed that most MaSSADHs are passively responsive to low-temperature treatment. In addition, transient overexpression of MaSSADH1-14 in Nicotiana benthamiana leaves resulted in the content of GABA increasing, indicating that MaSSADH1-14 may be involved in the accumulation of GABA of banana. Collectively, these results improve knowledge of the SSADH gene family in banana and establish a basis for comprehending its biological roles in response to low temperatures.

Keyword :

banana (Musa app.) banana (Musa app.) low temperature stress low temperature stress succinic semialdehyde dehydrogenase succinic semialdehyde dehydrogenase

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GB/T 7714 Guo, Xiong , Yang, Fengjie , Zhang, Xueying et al. Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (7) .
MLA Guo, Xiong et al. "Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 7 (2025) .
APA Guo, Xiong , Yang, Fengjie , Zhang, Xueying , Tang, Mengjie , Wan, Kui , Lai, Chunwang et al. Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (7) .
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Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter SCIE
期刊论文 | 2025 , 14 (3) | PLANTS-BASEL
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Betalains differ from anthocyanins, and they cannot coexist in the same plant under natural conditions. The L-DOPA 4,5-dioxygenase encoded by the DODA gene is a key step in the pathway of betalain biosynthesis in Caryophyllales plants. Amaranth is an important resource for the study and extraction of betalains. In order to clarify the function of AtrDODA family genes in betalain biosynthesis, we screened out three AtrDODA family gene members associated with betalains based on a genome database and RNA-seq databases of Amaranthus tricolor. Their characterization and expression pattern were further analyzed. The result of subcellular localization showed that all three AtrDODA members were located in the nucleus. Betacyanin and betaxanthin were promoted by paclobutrazol treatment in the leaves and stems of 'Suxian No.1' (red), while they were inhibited by gibberellin and darkness, which were consistent with the gene expression pattern of AtrDODAs. After heterologous transformation of the AtrDODA1-1 promoter into tobacco with GUS staining analysis, the promoter activity of AtrDODA1-1 of 'Suxian No.1' (red) amaranth was significantly higher than that of 'Suxian No.2' (green) amaranth. Furthermore, we analyzed the promoter activity of AtrDODA1-1 by GUS staining and qRT-PCR after sprayed exogenous MeJA and GA3 on the AtrDODA1-1 promoter transformed tobacco plants. The results showed that AtrDODA1-1 responded to plant hormones. This study could lay a foundation for revealing the biological functions of the amaranth DODA gene family, and provide new clues for the molecular mechanism of betalain synthesis.

Keyword :

Amaranthus tricolor L Amaranthus tricolor L betalain betalain DODA gene family DODA gene family expression analysis expression analysis promoter activity promoter activity

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GB/T 7714 Xie, Huiying , Zeng, Jiajing , Feng, Wenli et al. Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter [J]. | PLANTS-BASEL , 2025 , 14 (3) .
MLA Xie, Huiying et al. "Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter" . | PLANTS-BASEL 14 . 3 (2025) .
APA Xie, Huiying , Zeng, Jiajing , Feng, Wenli , Gao, Wei , Lai, Zhongxiong , Liu, Shengcai . Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter . | PLANTS-BASEL , 2025 , 14 (3) .
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Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour. SCIE
期刊论文 | 2025 , 14 (3) | PLANTS-BASEL
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Dimocarpus longan Lour. is an evergreen tree of the genus Longan in the Sapindaceae family, native to tropical and subtropical regions. Longan embryonic development is closely related to fruit set and fruit quality. An in-depth study of the mechanism of longan embryonic development could therefore contribute to the development of the longan industry. DIMBOA is the principal compound representing benzoxazinoids (BXs), and is closely linked to auxin biosynthesis and signal transduction. Auxin is one of the crucial hormones for inducing somatic embryogenesis (SE) in plants. Previous research has shown that DIMBOA promotes morphogenesis in the early somatic embryogenesis of longan, but the specific regulatory mechanism has not yet been clarified. To elucidate the molecular mechanism by which DIMBOA affects early somatic embryogenesis in longan, we chose longan embryogenic cultures grown under 0 mg/L DIMBOA as the control group (the check, CK), and longan embryogenic cultures grown under 0.1 mg/L DIMBOA as the treatment group (D) to be analyzed by transcriptomic sequencing. A total of 478 differentially expressed genes (DEGs) are detected in check vs. D, of which 193 are upregulated and 285 are downregulated. These DEGs are significantly enriched in the biosynthetic and metabolic functions of various substances such as vitamin B6 (VB6) biosynthesis, phenylpropanoid pathways, and carbohydrate metabolism. DIMBOA affects SE processes in longan via TFs, including MYB, ZF, bHLH, LBD, NAC, WRKY, etc. After DIMBOA treatment, the expression of most of the key genes for IAA synthesis was significantly downregulated, VB6 content was significantly reduced, and H2O2 content was significantly increased. Therefore, it is suggested that DIMBOA directly or indirectly affects the H2O2 content through the VB6 metabolic pathway, thereby regulating the endogenous IAA level to modulate the early SE morphogenesis of longan.

Keyword :

DIMBOA DIMBOA Dimocarpus longan Lour. Dimocarpus longan Lour. RNA-seq RNA-seq somatic embryogenesis somatic embryogenesis vitamin B-6 vitamin B-6

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GB/T 7714 Xu, Xiaoqiong , Zhang, Chunyu , Tong, Ning et al. Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour. [J]. | PLANTS-BASEL , 2025 , 14 (3) .
MLA Xu, Xiaoqiong et al. "Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour." . | PLANTS-BASEL 14 . 3 (2025) .
APA Xu, Xiaoqiong , Zhang, Chunyu , Tong, Ning , Lan, Xiaoyuan , Cui, Jing , Muhammad, Awais et al. Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour. . | PLANTS-BASEL , 2025 , 14 (3) .
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Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression SCIE
期刊论文 | 2025 , 14 (3) | PLANTS-BASEL
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MYB (myeloblastosis) is one of the most abundant transcription factors in plants which regulates various biological processes. The molecular characteristics and function of R2R3-MYB transcription factors in amaranth remain unclear. In this study, 73 R2R3-MYB members were identified from the amaranth genome database and we further analyzed their chromosome position, conserved motifs, physiological and biochemical features, collinearity relationships, gene structure, phylogeny and cis-acting element. Based on the phylogenetic and expression pattern analysis, 14 candidate R2R3-MYB genes might be involved in the betalain synthesis. Amongst the 14 candidate R2R3-MYB genes, the expression level of AtrMYB72 was higher in 'Suxian No.1' than 'Suxian No.2', and also higher in the red section than in the green section of the same leaf in Amaranthus. The overexpression vector pCambia1301-AtrMYB72-GUS and VIGS (virus-induced gene silencing) vector pTRV2- AtrMYB72 were transferred into leaves of 'Suxian No.1' via an Agrobacterium-mediated method. The results showed that AtrMYB72 overexpression could promote betalain synthesis. A yeast one-hybrid assay and dual luciferase reporter gene assay demonstrated that AtrMYB72 could bind to the AtrCYP76AD1 promoter to promote betalain synthesis. These results indicated that AtrMYB72 promoted betalain biosynthesis in amaranth by activating the AtrCYP76AD1 transcription. Our results could provide new insights into the betalain biosynthesis in amaranth.

Keyword :

Amaranthus tricolor L. Amaranthus tricolor L. AtrMYB72 AtrMYB72 betalain biosynthesis betalain biosynthesis

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GB/T 7714 Xue, Yuwei , Li, Kexuan , Feng, Wenli et al. Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression [J]. | PLANTS-BASEL , 2025 , 14 (3) .
MLA Xue, Yuwei et al. "Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression" . | PLANTS-BASEL 14 . 3 (2025) .
APA Xue, Yuwei , Li, Kexuan , Feng, Wenli , Lai, Zhongxiong , Liu, Shengcai . Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression . | PLANTS-BASEL , 2025 , 14 (3) .
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Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) SCIE
期刊论文 | 2025 , 222 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
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Heat and drought are the stressors with significant adverse impacts on the yield stability of tea plants. The heat shock proteins 60 (HSP60s) play important roles in protecting plants under heat stress. However, the mechanism of HSP60s under heat and drought stresses remains unclear. Here, we identified 19 CsHSP60s (namely CsHSP60-1 to CsHSP60-19) in tea plants and classified them into three groups based on phylogenetic analysis. In addition, studies on gene duplication events during the evolutionary process demonstrated that CsHSP60 members were subjected to purify selection. Analysis of cis-acting elements revealed the presence of numerous stress and hormone-responsive elements within the promoter regions of CsHSP60s. Real-time quantitative fluorescent PCR (qRT-PCR) analyses demonstrated that CsHSP60s rapidly responded to heat and combined heat and drought stress while exhibiting a delayed response to drought stress. The inhibition of eight CsHSP60 genes via antisense oligodeoxynucleotide (AsODN) resulted in more severe damage and ROS accumulation. Specifically, CsHSP60-9, CsHSP60-16, and CsHSP60-19 exhibited notable reductions in Fv/Fm values and displayed increased accumulation of H2O2 and O2 & sdot;-. These observations indicated a potential role for CsHSP60 in mitigating ROS accumulation under stress conditions, thereby enhancing tea plants' resilience to heat and drought stresses. Using a yeast two- hybrid (Y2H) assay, we identified that CsHSP60-2 and CsHSP60-16 physically interact with CsCPN10-4 and CsCPN10-5, respectively. These interactions suggest a cooperative chaperone activity between CsHSP60 and CsCPN10 in response to combined heat and drought stress. These findings lay a foundation for further understanding the involvement of HSP60s in the tolerance mechanisms to compound heat and drought stresses.

Keyword :

Camellia sinensis Camellia sinensis Combined heat and drought stress Combined heat and drought stress CPN10 CPN10 Genome-wide analysis Genome-wide analysis HSP60 HSP60

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GB/T 7714 Zheng, Anru , Tian, Caiyun , Zhou, Chengzhe et al. Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 .
MLA Zheng, Anru et al. "Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis)" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 222 (2025) .
APA Zheng, Anru , Tian, Caiyun , Zhou, Chengzhe , Yang, Niannian , Wen, Shengjing , Hu, Xiaowen et al. Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 .
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Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) SCIE
期刊论文 | 2025 , 14 (5) | PLANTS-BASEL
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The regulatory mechanisms underlying embryogenic callus (EC) formation in polyploid bananas remain unexplored, posing challenges for genetic transformation and biotechnological applications. Here, we conducted transcriptome sequencing on cultured explants, non-embryogenic callus, EC, and browning callus in the ABB cultivar 'MJ' (Musa spp. cv. Bengal). Our analysis of differentially expressed genes (DEGs) revealed significant enrichment in plant hormones, MAPK, and zeatin biosynthesis pathways. Notably, most genes in the MJ variety exhibited balanced expression of the A and B alleles, but A-specific allele expression was dominant in the key signaling pathways, whereas B-specific allele expression was very rare during EC induction. In the auxin signaling pathway, six A-specific MJARF genes were markedly downregulated, underscoring their critical roles in the negative regulation of callus formation. Additionally, six A-specific MJEIN3 alleles were found to play negative regulatory roles in ethylene signaling during EC development. We also identified phenylpropanoids responsible for enzymatic browning. Furthermore, the expression patterns of transcription factors in bananas exhibited specific expression modes, highlighting the unique mechanisms of callus formation. This study enhanced our understanding of the regulatory roles of these alleles in EC induction and offers new insights into the utilization of alleles to improve the efficiency of somatic embryogenesis in bananas.

Keyword :

browning browning embryogenic callus embryogenic callus specific allele specific allele transcriptional regulation transcriptional regulation

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GB/T 7714 Zhao, Xiaobing , Zhuang, Yiting , Xie, Wangyang et al. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) [J]. | PLANTS-BASEL , 2025 , 14 (5) .
MLA Zhao, Xiaobing et al. "Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group)" . | PLANTS-BASEL 14 . 5 (2025) .
APA Zhao, Xiaobing , Zhuang, Yiting , Xie, Wangyang , Yang, Yixin , Pu, Jingyu , Fan, Zhengyang et al. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) . | PLANTS-BASEL , 2025 , 14 (5) .
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The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan SCIE
期刊论文 | 2025 , 197 (3) | PLANT PHYSIOLOGY
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Heat stress can seriously affect plant growth and development. Ethylene response factors (ERFs) play important roles in plant development and physiological responses. Here, we identified DlERF6, an ERF family transcription factor that promotes heat tolerance in Dimocarpus longan. DlERF6 was strongly induced by heat stress and IAA treatment in longan roots. Overexpression of DlERF6 generated abundant, fast-growing hairy roots and enhanced longan heat stress tolerance by promoting IAA biosynthesis and reactive oxygen species (ROS) scavenging. Additional assays indicated that DlERF6 directly binds to the DlGH3.5 promoter and represses its expression. Overexpressing DlGH3.5 reduced hairy root number, root length, and heat tolerance, concomitant with a reduction in IAA content and ROS scavenging. Collectively, these results reveal the molecular mechanism through which the DlERF6-DlGH3.5 module regulates root growth and heat stress tolerance, providing a gene network that can be used for the genetic improvement of longan. An ethylene response factor regulates longan root growth and heat tolerance by modulating reactive oxygen species and auxin homeostasis.

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GB/T 7714 Zhang, Xueying , Zhang, Shuting , Wang, Shuangjie et al. The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan [J]. | PLANT PHYSIOLOGY , 2025 , 197 (3) .
MLA Zhang, Xueying et al. "The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan" . | PLANT PHYSIOLOGY 197 . 3 (2025) .
APA Zhang, Xueying , Zhang, Shuting , Wang, Shuangjie , Ma, Wentao , Zhai, Tingkai , Gao, Jie et al. The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan . | PLANT PHYSIOLOGY , 2025 , 197 (3) .
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