• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
  • DOI
  • UT
成果搜索
High Impact Results & Cited Count Trend for Year Keyword Cloud and Partner Relationship

Query:

学者姓名:赖钟雄

Refining:

Source

Submit Unfold

Co-Author

Submit Unfold

Language

Submit

Clean All

Sort by:
Default
  • Default
  • Title
  • Year
  • WOS Cited Count
  • Impact factor
  • Ascending
  • Descending
< Page ,Total 94 >
Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter SCIE
期刊论文 | 2025 , 14 (3) | PLANTS-BASEL
Abstract&Keyword Cite

Abstract :

Betalains differ from anthocyanins, and they cannot coexist in the same plant under natural conditions. The L-DOPA 4,5-dioxygenase encoded by the DODA gene is a key step in the pathway of betalain biosynthesis in Caryophyllales plants. Amaranth is an important resource for the study and extraction of betalains. In order to clarify the function of AtrDODA family genes in betalain biosynthesis, we screened out three AtrDODA family gene members associated with betalains based on a genome database and RNA-seq databases of Amaranthus tricolor. Their characterization and expression pattern were further analyzed. The result of subcellular localization showed that all three AtrDODA members were located in the nucleus. Betacyanin and betaxanthin were promoted by paclobutrazol treatment in the leaves and stems of 'Suxian No.1' (red), while they were inhibited by gibberellin and darkness, which were consistent with the gene expression pattern of AtrDODAs. After heterologous transformation of the AtrDODA1-1 promoter into tobacco with GUS staining analysis, the promoter activity of AtrDODA1-1 of 'Suxian No.1' (red) amaranth was significantly higher than that of 'Suxian No.2' (green) amaranth. Furthermore, we analyzed the promoter activity of AtrDODA1-1 by GUS staining and qRT-PCR after sprayed exogenous MeJA and GA3 on the AtrDODA1-1 promoter transformed tobacco plants. The results showed that AtrDODA1-1 responded to plant hormones. This study could lay a foundation for revealing the biological functions of the amaranth DODA gene family, and provide new clues for the molecular mechanism of betalain synthesis.

Keyword :

Amaranthus tricolor L Amaranthus tricolor L betalain betalain DODA gene family DODA gene family expression analysis expression analysis promoter activity promoter activity

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Xie, Huiying , Zeng, Jiajing , Feng, Wenli et al. Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter [J]. | PLANTS-BASEL , 2025 , 14 (3) .
MLA Xie, Huiying et al. "Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter" . | PLANTS-BASEL 14 . 3 (2025) .
APA Xie, Huiying , Zeng, Jiajing , Feng, Wenli , Gao, Wei , Lai, Zhongxiong , Liu, Shengcai . Differential Expression of Amaranth AtrDODA Gene Family Members in Betalain Synthesis and Functional Analysis of AtrDODA1-1 Promoter . | PLANTS-BASEL , 2025 , 14 (3) .
Export to NoteExpress RIS BibTex

Version :

Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour. SCIE
期刊论文 | 2025 , 14 (3) | PLANTS-BASEL
Abstract&Keyword Cite

Abstract :

Dimocarpus longan Lour. is an evergreen tree of the genus Longan in the Sapindaceae family, native to tropical and subtropical regions. Longan embryonic development is closely related to fruit set and fruit quality. An in-depth study of the mechanism of longan embryonic development could therefore contribute to the development of the longan industry. DIMBOA is the principal compound representing benzoxazinoids (BXs), and is closely linked to auxin biosynthesis and signal transduction. Auxin is one of the crucial hormones for inducing somatic embryogenesis (SE) in plants. Previous research has shown that DIMBOA promotes morphogenesis in the early somatic embryogenesis of longan, but the specific regulatory mechanism has not yet been clarified. To elucidate the molecular mechanism by which DIMBOA affects early somatic embryogenesis in longan, we chose longan embryogenic cultures grown under 0 mg/L DIMBOA as the control group (the check, CK), and longan embryogenic cultures grown under 0.1 mg/L DIMBOA as the treatment group (D) to be analyzed by transcriptomic sequencing. A total of 478 differentially expressed genes (DEGs) are detected in check vs. D, of which 193 are upregulated and 285 are downregulated. These DEGs are significantly enriched in the biosynthetic and metabolic functions of various substances such as vitamin B6 (VB6) biosynthesis, phenylpropanoid pathways, and carbohydrate metabolism. DIMBOA affects SE processes in longan via TFs, including MYB, ZF, bHLH, LBD, NAC, WRKY, etc. After DIMBOA treatment, the expression of most of the key genes for IAA synthesis was significantly downregulated, VB6 content was significantly reduced, and H2O2 content was significantly increased. Therefore, it is suggested that DIMBOA directly or indirectly affects the H2O2 content through the VB6 metabolic pathway, thereby regulating the endogenous IAA level to modulate the early SE morphogenesis of longan.

Keyword :

DIMBOA DIMBOA Dimocarpus longan Lour. Dimocarpus longan Lour. RNA-seq RNA-seq somatic embryogenesis somatic embryogenesis vitamin B-6 vitamin B-6

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Xu, Xiaoqiong , Zhang, Chunyu , Tong, Ning et al. Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour. [J]. | PLANTS-BASEL , 2025 , 14 (3) .
MLA Xu, Xiaoqiong et al. "Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour." . | PLANTS-BASEL 14 . 3 (2025) .
APA Xu, Xiaoqiong , Zhang, Chunyu , Tong, Ning , Lan, Xiaoyuan , Cui, Jing , Muhammad, Awais et al. Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis in Dimocarpus longan Lour. . | PLANTS-BASEL , 2025 , 14 (3) .
Export to NoteExpress RIS BibTex

Version :

Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression SCIE
期刊论文 | 2025 , 14 (3) | PLANTS-BASEL
Abstract&Keyword Cite

Abstract :

MYB (myeloblastosis) is one of the most abundant transcription factors in plants which regulates various biological processes. The molecular characteristics and function of R2R3-MYB transcription factors in amaranth remain unclear. In this study, 73 R2R3-MYB members were identified from the amaranth genome database and we further analyzed their chromosome position, conserved motifs, physiological and biochemical features, collinearity relationships, gene structure, phylogeny and cis-acting element. Based on the phylogenetic and expression pattern analysis, 14 candidate R2R3-MYB genes might be involved in the betalain synthesis. Amongst the 14 candidate R2R3-MYB genes, the expression level of AtrMYB72 was higher in 'Suxian No.1' than 'Suxian No.2', and also higher in the red section than in the green section of the same leaf in Amaranthus. The overexpression vector pCambia1301-AtrMYB72-GUS and VIGS (virus-induced gene silencing) vector pTRV2- AtrMYB72 were transferred into leaves of 'Suxian No.1' via an Agrobacterium-mediated method. The results showed that AtrMYB72 overexpression could promote betalain synthesis. A yeast one-hybrid assay and dual luciferase reporter gene assay demonstrated that AtrMYB72 could bind to the AtrCYP76AD1 promoter to promote betalain synthesis. These results indicated that AtrMYB72 promoted betalain biosynthesis in amaranth by activating the AtrCYP76AD1 transcription. Our results could provide new insights into the betalain biosynthesis in amaranth.

Keyword :

Amaranthus tricolor L. Amaranthus tricolor L. AtrMYB72 AtrMYB72 betalain biosynthesis betalain biosynthesis

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Xue, Yuwei , Li, Kexuan , Feng, Wenli et al. Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression [J]. | PLANTS-BASEL , 2025 , 14 (3) .
MLA Xue, Yuwei et al. "Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression" . | PLANTS-BASEL 14 . 3 (2025) .
APA Xue, Yuwei , Li, Kexuan , Feng, Wenli , Lai, Zhongxiong , Liu, Shengcai . Identification of R2R3-MYB Transcription Factor Family Based on Amaranthus tricolor Genome and AtrMYB72 Promoting Betalain Biosynthesis by Directly Activating AtrCYP76AD1 Expression . | PLANTS-BASEL , 2025 , 14 (3) .
Export to NoteExpress RIS BibTex

Version :

Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) SCIE
期刊论文 | 2025 , 14 (5) | PLANTS-BASEL
Abstract&Keyword Cite

Abstract :

The regulatory mechanisms underlying embryogenic callus (EC) formation in polyploid bananas remain unexplored, posing challenges for genetic transformation and biotechnological applications. Here, we conducted transcriptome sequencing on cultured explants, non-embryogenic callus, EC, and browning callus in the ABB cultivar 'MJ' (Musa spp. cv. Bengal). Our analysis of differentially expressed genes (DEGs) revealed significant enrichment in plant hormones, MAPK, and zeatin biosynthesis pathways. Notably, most genes in the MJ variety exhibited balanced expression of the A and B alleles, but A-specific allele expression was dominant in the key signaling pathways, whereas B-specific allele expression was very rare during EC induction. In the auxin signaling pathway, six A-specific MJARF genes were markedly downregulated, underscoring their critical roles in the negative regulation of callus formation. Additionally, six A-specific MJEIN3 alleles were found to play negative regulatory roles in ethylene signaling during EC development. We also identified phenylpropanoids responsible for enzymatic browning. Furthermore, the expression patterns of transcription factors in bananas exhibited specific expression modes, highlighting the unique mechanisms of callus formation. This study enhanced our understanding of the regulatory roles of these alleles in EC induction and offers new insights into the utilization of alleles to improve the efficiency of somatic embryogenesis in bananas.

Keyword :

browning browning embryogenic callus embryogenic callus specific allele specific allele transcriptional regulation transcriptional regulation

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Zhao, Xiaobing , Zhuang, Yiting , Xie, Wangyang et al. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) [J]. | PLANTS-BASEL , 2025 , 14 (5) .
MLA Zhao, Xiaobing et al. "Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group)" . | PLANTS-BASEL 14 . 5 (2025) .
APA Zhao, Xiaobing , Zhuang, Yiting , Xie, Wangyang , Yang, Yixin , Pu, Jingyu , Fan, Zhengyang et al. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) . | PLANTS-BASEL , 2025 , 14 (5) .
Export to NoteExpress RIS BibTex

Version :

Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) SCIE
期刊论文 | 2025 , 222 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
Abstract&Keyword Cite

Abstract :

Heat and drought are the stressors with significant adverse impacts on the yield stability of tea plants. The heat shock proteins 60 (HSP60s) play important roles in protecting plants under heat stress. However, the mechanism of HSP60s under heat and drought stresses remains unclear. Here, we identified 19 CsHSP60s (namely CsHSP60-1 to CsHSP60-19) in tea plants and classified them into three groups based on phylogenetic analysis. In addition, studies on gene duplication events during the evolutionary process demonstrated that CsHSP60 members were subjected to purify selection. Analysis of cis-acting elements revealed the presence of numerous stress and hormone-responsive elements within the promoter regions of CsHSP60s. Real-time quantitative fluorescent PCR (qRT-PCR) analyses demonstrated that CsHSP60s rapidly responded to heat and combined heat and drought stress while exhibiting a delayed response to drought stress. The inhibition of eight CsHSP60 genes via antisense oligodeoxynucleotide (AsODN) resulted in more severe damage and ROS accumulation. Specifically, CsHSP60-9, CsHSP60-16, and CsHSP60-19 exhibited notable reductions in Fv/Fm values and displayed increased accumulation of H2O2 and O2 & sdot;-. These observations indicated a potential role for CsHSP60 in mitigating ROS accumulation under stress conditions, thereby enhancing tea plants' resilience to heat and drought stresses. Using a yeast two- hybrid (Y2H) assay, we identified that CsHSP60-2 and CsHSP60-16 physically interact with CsCPN10-4 and CsCPN10-5, respectively. These interactions suggest a cooperative chaperone activity between CsHSP60 and CsCPN10 in response to combined heat and drought stress. These findings lay a foundation for further understanding the involvement of HSP60s in the tolerance mechanisms to compound heat and drought stresses.

Keyword :

Camellia sinensis Camellia sinensis Combined heat and drought stress Combined heat and drought stress CPN10 CPN10 Genome-wide analysis Genome-wide analysis HSP60 HSP60

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Zheng, Anru , Tian, Caiyun , Zhou, Chengzhe et al. Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 .
MLA Zheng, Anru et al. "Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis)" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 222 (2025) .
APA Zheng, Anru , Tian, Caiyun , Zhou, Chengzhe , Yang, Niannian , Wen, Shengjing , Hu, Xiaowen et al. Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 .
Export to NoteExpress RIS BibTex

Version :

Transcriptome analysis reveals that regulation network of the genes related to unique double flowers in tropical viviparous water lily SCIE
期刊论文 | 2025 , 15 (1) | SCIENTIFIC REPORTS
Abstract&Keyword Cite

Abstract :

Among tropical viviparous water lilies (Nymphaea L.), double-petaled cultivars are exceptionally rare, and the molecular mechanisms underlying their floral organ development remain largely unexplored. In this study, we identified Nymphaea 'Candy Rain' as the sole known tropical water lily cultivar exhibiting a double-petaled phenotype, characterized by complete petaloid conversion of innermost stamens. To elucidate the genetic basis of this unique floral morphology, we performed comparative transcriptome analysis of three distinct petal types in N. 'Candy Rain'. Our investigation further extended to comprehensive identification and characterization of MADS-box gene family members in the reference genome of the tropical viviparous water lily Nymphaea 'Paul Stetson'. Genome-wide analysis revealed 65 NcMADS-box genes unevenly distributed across 25 chromosomes. Phylogenetic and expression analyses implicated several MADS-box gene clades (particularly AGL6, AP2, and AG) in the development of the double-petaled phenotype. Additionally, our data suggest potential involvement of other transcription factor families including WOX, NAC, TCP, MYB, and bHLH in this specialized floral morphogenesis. Notably, we identified multiple phytohormone-related genes (such as IAA16, YUC6, and TCH4) that may participate in the formation of double-petaled flowers, indicating complex hormonal regulation of this trait in tropical viviparous water lilies.

Keyword :

ABCE gene model ABCE gene model Double flower Double flower MADS-box gene family MADS-box gene family Stamen petaloid Stamen petaloid Tropical viviparous water lily Tropical viviparous water lily

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Su, Qun , Tian, Min , Wang, Hongyan et al. Transcriptome analysis reveals that regulation network of the genes related to unique double flowers in tropical viviparous water lily [J]. | SCIENTIFIC REPORTS , 2025 , 15 (1) .
MLA Su, Qun et al. "Transcriptome analysis reveals that regulation network of the genes related to unique double flowers in tropical viviparous water lily" . | SCIENTIFIC REPORTS 15 . 1 (2025) .
APA Su, Qun , Tian, Min , Wang, Hongyan , Huang, Zhanwen , Awais, Muhammad , Xu, Xiaoqing et al. Transcriptome analysis reveals that regulation network of the genes related to unique double flowers in tropical viviparous water lily . | SCIENTIFIC REPORTS , 2025 , 15 (1) .
Export to NoteExpress RIS BibTex

Version :

龙眼DlAGO7基因克隆与亚细胞定位及表达分析
期刊论文 | 2025 , 23 (02) , 380-386 | 分子植物育种
Abstract&Keyword Cite

Abstract :

Argonaute7 (AGO7)在植物叶和花发育过程中发挥着重要的调控作用,而AGO7蛋白如何参与调控龙眼体胚的发育却未见报道。为了深入研究龙眼AGO7基因的功能与调控作用,本研究基于龙眼基因组数据库,采用RT-PCR法克隆龙眼DlAGO7基因,对其进行生物信息学分析,构建瞬时融合表达载体,明确其亚细胞定位,最后,利用实时荧光定量仪检测其在龙眼体胚不同阶段的相对表达情况。克隆获得DlAGO7的CDS序列为3 060 bp,编码1 019个氨基酸;所编码氨基酸序列含有PAZ和Piwi高度保守结构域;blastp序列比对分析表明,DlAGO7蛋白与其他植物的AGO7蛋白具有高度同源性;系统进化发育树分析显示,DlAGO7蛋白与甜橙AGO7蛋白亲缘关系最近,与单子叶植物玉米和水稻的亲缘关系最远;由亚细胞定位研究可知,龙眼DlAGO7基因为核定位基因;RT-qPCR分析表明,DlAGO7在龙眼球形胚阶段显著表达;克隆获得DlAGO7基因的5′端上游启动子序列长度为1 746 bp,启动子分析共预测出9类顺式作用元件。由此推测,DlAGO7基因可能在龙眼球形胚阶段发挥着重要的调控作用,该基因主要在细胞核中发挥作用。以上研究结果可为龙眼的分子育种提供理论基础。

Keyword :

AGO7基因 AGO7基因 亚细胞定位 亚细胞定位 启动子序列 启动子序列 龙眼体胚发生 龙眼体胚发生

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 陈荣珠 , 王小平 , 林美珍 et al. 龙眼DlAGO7基因克隆与亚细胞定位及表达分析 [J]. | 分子植物育种 , 2025 , 23 (02) : 380-386 .
MLA 陈荣珠 et al. "龙眼DlAGO7基因克隆与亚细胞定位及表达分析" . | 分子植物育种 23 . 02 (2025) : 380-386 .
APA 陈荣珠 , 王小平 , 林美珍 , 赖钟雄 . 龙眼DlAGO7基因克隆与亚细胞定位及表达分析 . | 分子植物育种 , 2025 , 23 (02) , 380-386 .
Export to NoteExpress RIS BibTex

Version :

Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening SCIE
期刊论文 | 2025 , 150 (5) , 237-248 | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE
Abstract&Keyword Cite

Abstract :

In the life cycle of fruit, ripening is a crucial period for the formation of fruit quality. During this phase, a series of physiological and biochemical changes occur, leading to alterations in color and texture, softening, and the production of aroma volatiles. Relevant studies have demonstrated that ethylene-induced fruit ripening is a complex regulatory network involving numerous transcription factors, ethylene receptor protein families, signal transduction pathways, and downstream maturation-related genes. In this study, we conducted a genome-scale identification and characterization of long noncoding RNAs (lncRNAs) from the predominant AAA triploid banana cultivar, 'Tianbao-jiao', using rich RNA-sequencing (RNA-seq) datasets from fruit tissues treated with ethylene and untreated controls. This represents one of the first comprehensive investigations into banana lncRNAs associated with ripening. A total of 11,268 banana fruit lncRNAs were identified and found to be distributed across all banana chromosomes; similar to 76.1% of these lncRNAs were transcribed from intergenic regions. Banana fruit lncRNAs are relatively short, with 91.32% ranging from 100 to 1000 nucleotides in length. Most contain one or two exons, contrasting with protein-coding genes, which can have one to >= 10 exons. Compared with naturally ripened bananas, many lncRNAs exhibited significant differential expression in ethylene-treated banana fruits. Furthermore, downregulating the expression of lncRNA TCONS00507247 in the peel markedly inhibited the yellowing process. These findings suggest that lncRNAs play a vital role in regulating banana fruit ripening and offer new insights into fruit ripening research.

Keyword :

banana ripening banana ripening ethylene ethylene lncRNAs lncRNAs regulation regulation RNA interference RNA interference

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Huang, Yuji , Lin, Tong , Fang, Yina et al. Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening [J]. | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE , 2025 , 150 (5) : 237-248 .
MLA Huang, Yuji et al. "Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening" . | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 150 . 5 (2025) : 237-248 .
APA Huang, Yuji , Lin, Tong , Fang, Yina , Yuan, Lingfeng , Feng, Xu , Lin, Yuling et al. Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening . | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE , 2025 , 150 (5) , 237-248 .
Export to NoteExpress RIS BibTex

Version :

The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway SCIE
期刊论文 | 2025 , 223 | PLANT PHYSIOLOGY AND BIOCHEMISTRY
Abstract&Keyword Cite

Abstract :

The basic-leucine zipper (bZIP) transcription factor (TF), which is abundant and highly conserved in eukaryotes, mainly participates in plant growth, development and adverse stress processes. However, the regulatory mechanisms of bZIP TFs under heat stress in Dimocarpus longan remain unclear. In this study, we reported a nuclear-localized bZIP TF, DlbZIP20, which was confirmed to be a positive regulator of thermotolerance in D. longan. It was identified as a strong interplay with hormone signalling pathways and oxidoreductase-related families. The transient overexpression DlbZIP20 could improve the thermotolerance of D. longan embryogenic callus (EC) and reduce the damage of cells under heat stress. Overexpression of DlbZIP20 in hairy roots significantly promoted roots elongation and photosystem II (PS II) responses in leaves, and significantly increased SOD and POD activities, promoted H2O2 scavenging, improved thermotolerance of D. longan by maintaining ROS homeostasis under heat stress. In addition, overexpression of DlbZIP20 increased endogenous MeJA content under heat stress. MeJA hormone synthesis genes DlMYC2 and DlCOI1 were up-regulated and DlJAZ3 was down-regulated in expression. This signalling pathway may play a role in the regulation of thermotolerance in D. longan. These results will provide a new insight into the molecular function of bZIP20 TF in response to heat stress in D. longan.

Keyword :

Abiotic stress responses Abiotic stress responses Antioxidant system Antioxidant system Hairy root culture Hairy root culture Plant hormone interactions Plant hormone interactions

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Zhai, Tingkai , Guo, Yueru , Yang, Mengmeng et al. The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 .
MLA Zhai, Tingkai et al. "The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 223 (2025) .
APA Zhai, Tingkai , Guo, Yueru , Yang, Mengmeng , Zhang, Xueying , Lin, Yiyao , Cai, Danfeng et al. The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 .
Export to NoteExpress RIS BibTex

Version :

Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua SCIE
期刊论文 | 2025 , 230 | INDUSTRIAL CROPS AND PRODUCTS
Abstract&Keyword Cite

Abstract :

Polygonatum cyrtonema Hua (P. cyrtonema), a traditional medicinal herb, is renowned for its high metabolite content. Previous studies have found that APETALA2/Ethylene Responsive Factor (AP2/ERF) plays a vital role in the metabolisms of P. cyrtonema, while the regulatory mechanism of PcAP2/ERFs in the flavonoid accumulation remains unclear. A total of 53 PcAP2/ERFs were identified from the full-length transcriptome. The expression analysis of PcAP2/ERFs in different age section rhizomes and different tissue sections showed that PcERF114 was associated with flavonoid accumulation and located in the nucleus. The flavonoid content was significantly upregulated with the transient overexpression of PcERF114, and significantly down-regulated with virus-induced gene silencing of PcERF114 in the P. cyrtonema leaves. Meanwhile, an integrative analysis of transcriptome and metabolome revealed that differentially expressed genes and differentially accumulated metabolites of transgenic leaves were closely related to the flavonoid biosynthesis pathway, and PcCHS1 was identified as a flavonoid biosynthesis-related key gene by WGCNA, qRT-PCR, and KEGG analysis. Overexpression of PcERF114 in the stable transgenic hairy roots and transiently transformed rhizomes increased the PcCHS1 expression, and the transient co-transformation of overexpressing PcERF114 and PcCHS1 promoted flavonoid accumulation, suggesting that PcERF114 may affect the expression of PcCHS1, thereby promoting the flavonoid accumulation of P. cyrtonema. The results provided a reference for further functional studies of secondary metabolism biosynthesis-related genes in medicinal plants.

Keyword :

AP2/ERF AP2/ERF ERF114 ERF114 Flavonoids Flavonoids Metabolome Metabolome Polygonatum cyrtonema Hua Polygonatum cyrtonema Hua Transcriptome Transcriptome

Cite:

Copy from the list or Export to your reference management。

GB/T 7714 Wan, Kui , Yang, Fengjie , Nan, Yanlong et al. Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 230 .
MLA Wan, Kui et al. "Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua" . | INDUSTRIAL CROPS AND PRODUCTS 230 (2025) .
APA Wan, Kui , Yang, Fengjie , Nan, Yanlong , Zhang, Shuting , Huang, Zhixia , Chen, Yukun et al. Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 230 .
Export to NoteExpress RIS BibTex

Version :

10| 20| 50 per page
< Page ,Total 94 >

Export

Results:

Selected

to

Format:
Online/Total:163/15235
Address:FAFU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350002)
Copyright:FAFU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备10012082号