Query：
学者姓名：赖钟雄
Refining：
Year
Type
Indexed by
Source
Complex
Co-Author
Language
Clean All
Abstract ：
The regulatory mechanisms underlying embryogenic callus (EC) formation in polyploid bananas remain unexplored, posing challenges for genetic transformation and biotechnological applications. Here, we conducted transcriptome sequencing on cultured explants, non-embryogenic callus, EC, and browning callus in the ABB cultivar 'MJ' (Musa spp. cv. Bengal). Our analysis of differentially expressed genes (DEGs) revealed significant enrichment in plant hormones, MAPK, and zeatin biosynthesis pathways. Notably, most genes in the MJ variety exhibited balanced expression of the A and B alleles, but A-specific allele expression was dominant in the key signaling pathways, whereas B-specific allele expression was very rare during EC induction. In the auxin signaling pathway, six A-specific MJARF genes were markedly downregulated, underscoring their critical roles in the negative regulation of callus formation. Additionally, six A-specific MJEIN3 alleles were found to play negative regulatory roles in ethylene signaling during EC development. We also identified phenylpropanoids responsible for enzymatic browning. Furthermore, the expression patterns of transcription factors in bananas exhibited specific expression modes, highlighting the unique mechanisms of callus formation. This study enhanced our understanding of the regulatory roles of these alleles in EC induction and offers new insights into the utilization of alleles to improve the efficiency of somatic embryogenesis in bananas.
Keyword ：
browning browning embryogenic callus embryogenic callus specific allele specific allele transcriptional regulation transcriptional regulation
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhao, Xiaobing , Zhuang, Yiting , Xie, Wangyang et al. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) [J]. | PLANTS-BASEL , 2025 , 14 (5) . |
| MLA | Zhao, Xiaobing et al. "Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group)" . | PLANTS-BASEL 14 . 5 (2025) . |
| APA | Zhao, Xiaobing , Zhuang, Yiting , Xie, Wangyang , Yang, Yixin , Pu, Jingyu , Fan, Zhengyang et al. Allelic Expression Dynamics of Regulatory Factors During Embryogenic Callus Induction in ABB Banana (Musa spp. cv. Bengal, ABB Group) . | PLANTS-BASEL , 2025 , 14 (5) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Heat and drought are the stressors with significant adverse impacts on the yield stability of tea plants. The heat shock proteins 60 (HSP60s) play important roles in protecting plants under heat stress. However, the mechanism of HSP60s under heat and drought stresses remains unclear. Here, we identified 19 CsHSP60s (namely CsHSP60-1 to CsHSP60-19) in tea plants and classified them into three groups based on phylogenetic analysis. In addition, studies on gene duplication events during the evolutionary process demonstrated that CsHSP60 members were subjected to purify selection. Analysis of cis-acting elements revealed the presence of numerous stress and hormone-responsive elements within the promoter regions of CsHSP60s. Real-time quantitative fluorescent PCR (qRT-PCR) analyses demonstrated that CsHSP60s rapidly responded to heat and combined heat and drought stress while exhibiting a delayed response to drought stress. The inhibition of eight CsHSP60 genes via antisense oligodeoxynucleotide (AsODN) resulted in more severe damage and ROS accumulation. Specifically, CsHSP60-9, CsHSP60-16, and CsHSP60-19 exhibited notable reductions in Fv/Fm values and displayed increased accumulation of H2O2 and O2 & sdot;-. These observations indicated a potential role for CsHSP60 in mitigating ROS accumulation under stress conditions, thereby enhancing tea plants' resilience to heat and drought stresses. Using a yeast two- hybrid (Y2H) assay, we identified that CsHSP60-2 and CsHSP60-16 physically interact with CsCPN10-4 and CsCPN10-5, respectively. These interactions suggest a cooperative chaperone activity between CsHSP60 and CsCPN10 in response to combined heat and drought stress. These findings lay a foundation for further understanding the involvement of HSP60s in the tolerance mechanisms to compound heat and drought stresses.
Keyword ：
Camellia sinensis Camellia sinensis Combined heat and drought stress Combined heat and drought stress CPN10 CPN10 Genome-wide analysis Genome-wide analysis HSP60 HSP60
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zheng, Anru , Tian, Caiyun , Zhou, Chengzhe et al. Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 . |
| MLA | Zheng, Anru et al. "Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis)" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 222 (2025) . |
| APA | Zheng, Anru , Tian, Caiyun , Zhou, Chengzhe , Yang, Niannian , Wen, Shengjing , Hu, Xiaowen et al. Genome-wide identification and characterization of CsHSP60 gene family associated with heat and drought responses in tea plants (Camellia sinensis) . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 222 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Dormancy release is an important process for improving the quality of cut-flower lily production and promoting the factory production of lily bulbs. However, the regulatory mechanisms of microRNAs (miRNAs) and their target genes during the dormancy release of lily remain elusive. Anatomy, transcriptomic, molecular biology, and transient transformation techniques involving subcellular localization were applied in our study. There were significant results showing that 0.1 mM riboflavin promoted dormancy release and floral bud differentiation and influenced the flowering time of the Lilium 'Siberia'. Moreover, some differentially expressed miRNAs and their targets (miR395-y: LoAPS1, miR529-z: LoSPL14, miR396-y: LoCFDP1, miR1863-z: LoFBA3, miR399-y: LoDIT1, and miR11525-z: Lopgm) were identified and predicted. Exogenous riboflavin may activate primary metabolic processes and promote dormancy release in Lilium 'Siberia' bulbs. Furthermore, riboflavin upregulated genes related to the riboflavin pathway, H3K4me3 methylation, dormancy control, and the flowering pathway and downregulated dormancy maintenance genes. Moreover, riboflavin promoted endogenous riboflavin and acetyl-CoA accumulation. LoPurple acid phosphatase17 (LoPAP17), a pivotal gene of the riboflavin metabolism pathway, was subsequently cloned. LoPAP17 was most closely related to the orthologous genes of Acorus calamus, Asparagus officinalis, and Musa acuminata. The LoPAP17 protein was subcellularly located in the nucleus. Our study revealed that miRNAs and their target genes might regulate the primary metabolic pathway, promote the accumulation of endogenous riboflavin and acetyl-CoA, and affect protein acetylation during the riboflavin-promoted release of dormancy and flower bud differentiation in the Lilium Oriental hybrid 'Siberia'.
Keyword ：
dormancy release dormancy release Lilium Oriental hybrid 'Siberia' Lilium Oriental hybrid 'Siberia' miRNA miRNA riboflavin riboflavin transcriptomics transcriptomics
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Xu, Xiaoping , Yang, Chenglong , Zheng, Yiping et al. Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia' [J]. | HORTICULTURAE , 2025 , 11 (1) . |
| MLA | Xu, Xiaoping et al. "Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia'" . | HORTICULTURAE 11 . 1 (2025) . |
| APA | Xu, Xiaoping , Yang, Chenglong , Zheng, Yiping , Guo, Wenjie , Lai, Zhongxiong , Fang, Shaozhong . Transcriptome-Wide Identification of miRNAs and Their Targets During Riboflavin-Promoted Dormancy Release in Lilium 'Siberia' . | HORTICULTURAE , 2025 , 11 (1) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Heat stress can seriously affect plant growth and development. Ethylene response factors (ERFs) play important roles in plant development and physiological responses. Here, we identified DlERF6, an ERF family transcription factor that promotes heat tolerance in Dimocarpus longan. DlERF6 was strongly induced by heat stress and IAA treatment in longan roots. Overexpression of DlERF6 generated abundant, fast-growing hairy roots and enhanced longan heat stress tolerance by promoting IAA biosynthesis and reactive oxygen species (ROS) scavenging. Additional assays indicated that DlERF6 directly binds to the DlGH3.5 promoter and represses its expression. Overexpressing DlGH3.5 reduced hairy root number, root length, and heat tolerance, concomitant with a reduction in IAA content and ROS scavenging. Collectively, these results reveal the molecular mechanism through which the DlERF6-DlGH3.5 module regulates root growth and heat stress tolerance, providing a gene network that can be used for the genetic improvement of longan. An ethylene response factor regulates longan root growth and heat tolerance by modulating reactive oxygen species and auxin homeostasis.
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhang, Xueying , Zhang, Shuting , Wang, Shuangjie et al. The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan [J]. | PLANT PHYSIOLOGY , 2025 , 197 (3) . |
| MLA | Zhang, Xueying et al. "The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan" . | PLANT PHYSIOLOGY 197 . 3 (2025) . |
| APA | Zhang, Xueying , Zhang, Shuting , Wang, Shuangjie , Ma, Wentao , Zhai, Tingkai , Gao, Jie et al. The ETHYLENE RESPONSE FACTOR6-GRETCHEN HAGEN3.5 module regulates rooting and heat tolerance in Dimocarpus longan . | PLANT PHYSIOLOGY , 2025 , 197 (3) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The current investigation utilized stem nodes from pre-established aseptic lines of Mussaenda pubescens as explants to optimize an efficient in vitro propagation protocol and investigated the effect of different light qualities (white, red, blue, and green) on metabolite accumulation in micropropagated plantlets. The findings demonstrated that the optimal medium for shoot proliferation was Murashige and Skoog basal medium supplemented with 6-Benzylaminopurine 2.0 mgL-1 and alpha-naphthaleneacetic acid 0.2 mgL-1, achieving a multiplication coefficient of 12.2 after 30 days. Rooting was more effective on Murashige and Skoog basal medium containing alpha-naphthaleneacetic acid 0.1 mgL-1 and activated charcoal 1 gL-1, resulting in a 100% rooting rate. During acclimatization, a substrate mixture of perlite:vermiculite: peat soil (1:1:1) promoted vigorous root development with a 100% survival rate at post-transplantation. Light quality significantly influenced plant morphology: red light stimulated stem elongation, while blue light increased biomass accumulation. Broad-target metabolomics revealed distinct metabolite profiles under different light spectra, with differentially accumulated metabolites primarily belonging to terpenoids, organic acids, lipids, and flavonoids. Specifically, red light enhanced the levels of terpenoids and lipids; blue light promoted the synthesis of specific triterpenoid saponins and lipids; while green light increased the content of certain terpenes and broadly upregulated a wide spectrum of lipids. This work provides a robust framework for the commercial micropropagation of Mussaenda pubescens and elucidates the strategic use of light quality to enhance the production of its valuable medicinal metabolites, including terpenoids and lipids.
Keyword ：
bioactive metabolites bioactive metabolites in vitro regeneration in vitro regeneration LED spectrum LED spectrum medicinal value medicinal value metabolome profiling metabolome profiling Mussaenda pubescens Ait.f. Mussaenda pubescens Ait.f. secondary metabolites secondary metabolites
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Sun, Li , Wu, Jiajia , Yang, Zilu et al. Optimization of Micropropagation and Metabolomic Analysis Under Different Light Qualities in Mussaenda pubescens Ait.f [J]. | PLANTS-BASEL , 2025 , 14 (21) . |
| MLA | Sun, Li et al. "Optimization of Micropropagation and Metabolomic Analysis Under Different Light Qualities in Mussaenda pubescens Ait.f" . | PLANTS-BASEL 14 . 21 (2025) . |
| APA | Sun, Li , Wu, Jiajia , Yang, Zilu , Cai, Roudi , Xu, Xiaoping , Li, Jiahui et al. Optimization of Micropropagation and Metabolomic Analysis Under Different Light Qualities in Mussaenda pubescens Ait.f . | PLANTS-BASEL , 2025 , 14 (21) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Banana (Musa spp.) is susceptible to low-temperature stress and other environmental stresses, which can hinder the growth and development. Succinic semialdehyde dehydrogenase (SSADH) is critical for GABA biosynthesis and plays a crucial role in plants. However, the SSADH genes of bananas have not been studied. This study found 19 MaSSADHs, 18 MbSSADHs, and 18 MiSSADHs from the banana genome. According to the phylogenetic tree, these genes can be categorized into five branches. This study cloned the MaSSADH1-14 from banana. The subcellular localization assays of MaSSADH1-14 in tobacco leaves confirmed that the presence of SSADH was not only localized mitochondrion but also localized chloroplast. The cis-elements of the SSADH gene family are related to the potential regulation of the banana SSADH gene family; their involvement in diverse stress responses. Transcriptomic data was utilized to examine the effect of MaSSADH genes under cold stress in bananas. The results of RT-qPCR were consistent with transcriptome data. These results showed that most MaSSADHs are passively responsive to low-temperature treatment. In addition, transient overexpression of MaSSADH1-14 in Nicotiana benthamiana leaves resulted in the content of GABA increasing, indicating that MaSSADH1-14 may be involved in the accumulation of GABA of banana. Collectively, these results improve knowledge of the SSADH gene family in banana and establish a basis for comprehending its biological roles in response to low temperatures.
Keyword ：
banana (Musa app.) banana (Musa app.) low temperature stress low temperature stress succinic semialdehyde dehydrogenase succinic semialdehyde dehydrogenase
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Guo, Xiong , Yang, Fengjie , Zhang, Xueying et al. Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (7) . |
| MLA | Guo, Xiong et al. "Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 7 (2025) . |
| APA | Guo, Xiong , Yang, Fengjie , Zhang, Xueying , Tang, Mengjie , Wan, Kui , Lai, Chunwang et al. Genome-Wide Identification and Expression Analysis Unveil the Involvement of the Succinic Semialdehyde Dehydrogenase (SSADH) Gene Family in Banana Low Temperature Stress . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (7) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
The basic-leucine zipper (bZIP) transcription factor (TF), which is abundant and highly conserved in eukaryotes, mainly participates in plant growth, development and adverse stress processes. However, the regulatory mechanisms of bZIP TFs under heat stress in Dimocarpus longan remain unclear. In this study, we reported a nuclear-localized bZIP TF, DlbZIP20, which was confirmed to be a positive regulator of thermotolerance in D. longan. It was identified as a strong interplay with hormone signalling pathways and oxidoreductase-related families. The transient overexpression DlbZIP20 could improve the thermotolerance of D. longan embryogenic callus (EC) and reduce the damage of cells under heat stress. Overexpression of DlbZIP20 in hairy roots significantly promoted roots elongation and photosystem II (PS II) responses in leaves, and significantly increased SOD and POD activities, promoted H2O2 scavenging, improved thermotolerance of D. longan by maintaining ROS homeostasis under heat stress. In addition, overexpression of DlbZIP20 increased endogenous MeJA content under heat stress. MeJA hormone synthesis genes DlMYC2 and DlCOI1 were up-regulated and DlJAZ3 was down-regulated in expression. This signalling pathway may play a role in the regulation of thermotolerance in D. longan. These results will provide a new insight into the molecular function of bZIP20 TF in response to heat stress in D. longan.
Keyword ：
Abiotic stress responses Abiotic stress responses Antioxidant system Antioxidant system Hairy root culture Hairy root culture Plant hormone interactions Plant hormone interactions
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhai, Tingkai , Guo, Yueru , Yang, Mengmeng et al. The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway [J]. | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 . |
| MLA | Zhai, Tingkai et al. "The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway" . | PLANT PHYSIOLOGY AND BIOCHEMISTRY 223 (2025) . |
| APA | Zhai, Tingkai , Guo, Yueru , Yang, Mengmeng , Zhang, Xueying , Lin, Yiyao , Cai, Danfeng et al. The bZIP20 transcription factor enhances thermotolerance in Dimocarpus longan by maintaining ROS homeostasis and involving the MeJA pathway . | PLANT PHYSIOLOGY AND BIOCHEMISTRY , 2025 , 223 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
In the life cycle of fruit, ripening is a crucial period for the formation of fruit quality. During this phase, a series of physiological and biochemical changes occur, leading to alterations in color and texture, softening, and the production of aroma volatiles. Relevant studies have demonstrated that ethylene-induced fruit ripening is a complex regulatory network involving numerous transcription factors, ethylene receptor protein families, signal transduction pathways, and downstream maturation-related genes. In this study, we conducted a genome-scale identification and characterization of long noncoding RNAs (lncRNAs) from the predominant AAA triploid banana cultivar, 'Tianbao-jiao', using rich RNA-sequencing (RNA-seq) datasets from fruit tissues treated with ethylene and untreated controls. This represents one of the first comprehensive investigations into banana lncRNAs associated with ripening. A total of 11,268 banana fruit lncRNAs were identified and found to be distributed across all banana chromosomes; similar to 76.1% of these lncRNAs were transcribed from intergenic regions. Banana fruit lncRNAs are relatively short, with 91.32% ranging from 100 to 1000 nucleotides in length. Most contain one or two exons, contrasting with protein-coding genes, which can have one to >= 10 exons. Compared with naturally ripened bananas, many lncRNAs exhibited significant differential expression in ethylene-treated banana fruits. Furthermore, downregulating the expression of lncRNA TCONS00507247 in the peel markedly inhibited the yellowing process. These findings suggest that lncRNAs play a vital role in regulating banana fruit ripening and offer new insights into fruit ripening research.
Keyword ：
banana ripening banana ripening ethylene ethylene lncRNAs lncRNAs regulation regulation RNA interference RNA interference
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Huang, Yuji , Lin, Tong , Fang, Yina et al. Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening [J]. | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE , 2025 , 150 (5) : 237-248 . |
| MLA | Huang, Yuji et al. "Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening" . | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE 150 . 5 (2025) : 237-248 . |
| APA | Huang, Yuji , Lin, Tong , Fang, Yina , Yuan, Lingfeng , Feng, Xu , Lin, Yuling et al. Global Identification and Analysis of Long Noncoding RNAs Involved in Banana Fruit Ripening . | JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE , 2025 , 150 (5) , 237-248 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
GRAS family plays a critical role in plant growth and stress responses. In this study, we identified 47 GRAS (DlGRAS) genes in the longan genome and conducted a comprehensive bioinformatics analysis of these genes. RNA-seq analysis revealed that the expression of these DlGRAS genes differed during early SE and across various longan tissues. The quantitative real-time PCR (qRT-PCR) results indicated that the DlGRAS genes exhibited differential expression during the early SE of longan, with most of them showing high expression at the globular embryo (GE) stage. Under GA3 treatment, the transcript levels of DlGRAS12/15 decreased significantly. In contrast, exogenous ABA promoted the expression of DlGRAS6/10/23, indicating that DlGRAS genes are responsive to hormones. Compared with CaMV35S-driven GUS expression, the promoters of DlGRAS10/22 increased GUS expression, GA3 and ABA treatments enhanced promoter activity. DlGRAS10/22 were located in the nucleus. Overexpression of DlGRAS10/22 in longan SE significantly promoted the transcription levels of SE-related genes, including DlGID1, DlGA20ox2, DlLEC1, DlFUS3, DlABI3 and DlLEC2. Therefore, DlGRAS may be involved in the early morphogenesis of longan SE through the hormone signaling pathway.
Keyword ：
Dimocarpus longan Lour. Dimocarpus longan Lour. GRAS family GRAS family hormones responsive hormones responsive somatic embryogenesis somatic embryogenesis
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Zhao, Guanghui , Tang, Mengjie , Wu, Wanlong et al. Genome-Wide Identification of DlGRAS Family and Functional Analysis of DlGRAS10/22 Reveal Their Potential Roles in Embryogenesis and Hormones Responses in Dimocarpus longan [J]. | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (21) . |
| MLA | Zhao, Guanghui et al. "Genome-Wide Identification of DlGRAS Family and Functional Analysis of DlGRAS10/22 Reveal Their Potential Roles in Embryogenesis and Hormones Responses in Dimocarpus longan" . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 26 . 21 (2025) . |
| APA | Zhao, Guanghui , Tang, Mengjie , Wu, Wanlong , Gao, Wei , Xie, Jinbing , Wang, Jialing et al. Genome-Wide Identification of DlGRAS Family and Functional Analysis of DlGRAS10/22 Reveal Their Potential Roles in Embryogenesis and Hormones Responses in Dimocarpus longan . | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES , 2025 , 26 (21) . |
| Export to | NoteExpress RIS BibTex |
Version ：
Abstract ：
Polygonatum cyrtonema Hua (P. cyrtonema), a traditional medicinal herb, is renowned for its high metabolite content. Previous studies have found that APETALA2/Ethylene Responsive Factor (AP2/ERF) plays a vital role in the metabolisms of P. cyrtonema, while the regulatory mechanism of PcAP2/ERFs in the flavonoid accumulation remains unclear. A total of 53 PcAP2/ERFs were identified from the full-length transcriptome. The expression analysis of PcAP2/ERFs in different age section rhizomes and different tissue sections showed that PcERF114 was associated with flavonoid accumulation and located in the nucleus. The flavonoid content was significantly upregulated with the transient overexpression of PcERF114, and significantly down-regulated with virus-induced gene silencing of PcERF114 in the P. cyrtonema leaves. Meanwhile, an integrative analysis of transcriptome and metabolome revealed that differentially expressed genes and differentially accumulated metabolites of transgenic leaves were closely related to the flavonoid biosynthesis pathway, and PcCHS1 was identified as a flavonoid biosynthesis-related key gene by WGCNA, qRT-PCR, and KEGG analysis. Overexpression of PcERF114 in the stable transgenic hairy roots and transiently transformed rhizomes increased the PcCHS1 expression, and the transient co-transformation of overexpressing PcERF114 and PcCHS1 promoted flavonoid accumulation, suggesting that PcERF114 may affect the expression of PcCHS1, thereby promoting the flavonoid accumulation of P. cyrtonema. The results provided a reference for further functional studies of secondary metabolism biosynthesis-related genes in medicinal plants.
Keyword ：
AP2/ERF AP2/ERF ERF114 ERF114 Flavonoids Flavonoids Metabolome Metabolome Polygonatum cyrtonema Hua Polygonatum cyrtonema Hua Transcriptome Transcriptome
Cite：
Copy from the list or Export to your reference management。
| GB/T 7714 | Wan, Kui , Yang, Fengjie , Nan, Yanlong et al. Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua [J]. | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 230 . |
| MLA | Wan, Kui et al. "Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua" . | INDUSTRIAL CROPS AND PRODUCTS 230 (2025) . |
| APA | Wan, Kui , Yang, Fengjie , Nan, Yanlong , Zhang, Shuting , Huang, Zhixia , Chen, Yukun et al. Transcription factor PcERF114 is involved in the regulation of flavonoid accumulation of Polygonatum cyrtonema Hua . | INDUSTRIAL CROPS AND PRODUCTS , 2025 , 230 . |
| Export to | NoteExpress RIS BibTex |
Version ：
Export
| Results： |
Selected to |
| Format： |
